AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene049025


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene049025.t1 MTR_8g101900 90.837 251 7 1 1 251 1 235 1.12e-166 460
MS.gene049025.t1 MTR_8g021225 68.980 245 59 2 6 249 5 233 2.49e-118 338
MS.gene049025.t1 MTR_6g091680 66.397 247 67 1 5 251 8 238 9.53e-106 306
MS.gene049025.t1 MTR_4g085590 55.000 240 91 2 12 250 23 246 1.03e-92 273
MS.gene049025.t1 MTR_4g094925 51.029 243 102 2 9 250 16 242 5.28e-86 256
MS.gene049025.t1 MTR_4g094815 50.000 244 105 2 8 250 20 247 1.38e-85 255
MS.gene049025.t1 MTR_4g094898 51.230 244 102 2 8 250 20 247 1.09e-79 240
MS.gene049025.t1 MTR_4g094812 52.083 240 98 2 12 250 22 245 8.23e-77 233
MS.gene049025.t1 MTR_4g094368 49.167 240 102 4 12 248 5 227 2.02e-75 228
MS.gene049025.t1 MTR_4g094925 49.519 208 87 3 45 250 20 211 1.22e-70 216
MS.gene049025.t1 MTR_4g094898 51.485 202 81 2 50 250 1 186 1.07e-63 197
MS.gene049025.t1 MTR_2g070410 38.362 232 118 5 18 249 91 297 2.27e-44 151
MS.gene049025.t1 MTR_2g070410 38.298 188 96 3 18 205 91 258 2.14e-34 125
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene049025.t1 AT4G26220 61.088 239 77 1 12 250 9 231 1.84e-104 302
MS.gene049025.t1 AT4G34050 55.187 241 91 2 12 251 35 259 1.27e-92 273
MS.gene049025.t1 AT4G34050 55.187 241 91 2 12 251 62 286 1.83e-92 274
MS.gene049025.t1 AT1G67980 52.893 242 93 3 12 251 10 232 5.06e-84 251
MS.gene049025.t1 AT1G24735 52.263 243 95 4 12 251 16 240 9.18e-79 238
MS.gene049025.t1 AT1G67990 50.000 242 101 3 12 251 10 233 1.08e-76 232
MS.gene049025.t1 AT1G24735 43.345 293 94 5 12 250 16 290 1.52e-70 218
MS.gene049025.t1 AT1G24735 52.475 202 77 3 50 250 11 194 6.55e-67 206
MS.gene049025.t1 AT1G67980 55.556 144 61 1 107 250 23 163 8.16e-54 171
MS.gene049025.t1 AT4G34050 54.472 123 55 1 129 250 25 147 1.38e-44 147
MS.gene049025.t1 AT3G61990 39.545 220 108 5 30 249 95 289 3.82e-44 150
MS.gene049025.t1 AT3G62000 37.104 221 115 5 30 249 80 277 2.34e-42 145
MS.gene049025.t1 AT3G62000 35.545 211 112 5 30 239 80 267 1.47e-34 127

Find 44 sgRNAs with CRISPR-Local

Find 88 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTTCTGAAACTACCGTTTC+TGG 0.209949 4.1:+3458384 None:intergenic
CCATGAGAGGCTTATCAAAT+TGG 0.230744 4.1:-3458466 MS.gene049025:CDS
TCTGGATCTACGGCTGTAAT+GGG 0.325713 4.1:+3458754 None:intergenic
GTCTGGATCTACGGCTGTAA+TGG 0.340492 4.1:+3458753 None:intergenic
TCAAAAGTACCTTCATTTGC+AGG 0.353092 4.1:+3458531 None:intergenic
TGCCTACGATAACACCCTTT+GGG 0.369512 4.1:-3458424 MS.gene049025:CDS
GTAATTCTTCAGTGGCTTCA+TGG 0.374269 4.1:-3459286 MS.gene049025:intron
TCTAAAAGTTTATCAAGAAC+TGG 0.389451 4.1:+3458646 None:intergenic
ACCTGATGCGGGTCAGCTAA+TGG 0.395308 4.1:-3459256 MS.gene049025:CDS
ATACAAACCTTTCCATCATA+TGG 0.399941 4.1:+3459144 None:intergenic
TTGCCTACGATAACACCCTT+TGG 0.417902 4.1:-3458425 MS.gene049025:CDS
TTTCAGAAACAAAGAGAGAA+TGG 0.425659 4.1:-3458371 MS.gene049025:CDS
GACAGAAAAGCTTATGAGAT+AGG 0.427880 4.1:-3458733 MS.gene049025:CDS
TTATCAAGAACTGGCAAAGC+TGG 0.435178 4.1:+3458655 None:intergenic
GCCATTAGCTGACCCGCATC+AGG 0.436416 4.1:+3459255 None:intergenic
TAATTCTTCAGTGGCTTCAT+GGG 0.469413 4.1:-3459285 MS.gene049025:intron
ATCAAATTGGTTAAGATCGG+TGG 0.482512 4.1:-3458453 MS.gene049025:CDS
GCACTTAACATTCCATATGA+TGG 0.489413 4.1:-3459156 MS.gene049025:CDS
AACTGTTGCCTTGCCAGAAA+CGG 0.495235 4.1:-3458397 MS.gene049025:CDS
CTTATCAAATTGGTTAAGAT+CGG 0.511198 4.1:-3458456 MS.gene049025:CDS
TTGAGAGTCTCTGCTTCTCG+TGG 0.514299 4.1:+3459545 None:intergenic
CATGGGCACTTCACCTGATG+CGG 0.516416 4.1:-3459268 MS.gene049025:CDS
TTTCAATGAAGCAGTTTCAA+AGG 0.518882 4.1:-3458331 MS.gene049025:CDS
TGAAACTACCGTTTCTGGCA+AGG 0.521241 4.1:+3458389 None:intergenic
ATGGGCACTTCACCTGATGC+GGG 0.527395 4.1:-3459267 MS.gene049025:CDS
CTGCAAAATAACTGCATTGG+TGG 0.528279 4.1:+3459694 None:intergenic
CCAATTTGATAAGCCTCTCA+TGG 0.530119 4.1:+3458466 None:intergenic
TAACATTCCATATGATGGAA+AGG 0.530971 4.1:-3459151 MS.gene049025:intron
GCAGAGCGAGGGCTTACTCA+AGG 0.558409 4.1:-3459675 MS.gene049025:intron
TGCTTCTCGTGGGTAAACAC+TGG 0.559815 4.1:+3459556 None:intergenic
GGCAACAGTTCCTCCCCAAA+GGG 0.563275 4.1:+3458410 None:intergenic
AGGCAACAGTTCCTCCCCAA+AGG 0.565907 4.1:+3458409 None:intergenic
TCAAATTGGTTAAGATCGGT+GGG 0.567728 4.1:-3458452 MS.gene049025:CDS
TGAGAGTCTCTGCTTCTCGT+GGG 0.576132 4.1:+3459546 None:intergenic
GCTCTGCAAAATAACTGCAT+TGG 0.577755 4.1:+3459691 None:intergenic
GCGACGGACTCACTATCTGC+AGG 0.587918 4.1:-3458275 MS.gene049025:CDS
ACTATTGGAATTACCATGAG+AGG 0.588635 4.1:-3458479 MS.gene049025:CDS
TTGATGCAGCCTGCAAATGA+AGG 0.599302 4.1:-3458540 MS.gene049025:intron
GCCTACGATAACACCCTTTG+GGG 0.620350 4.1:-3458423 MS.gene049025:CDS
TACGATAACACCCTTTGGGG+AGG 0.624507 4.1:-3458420 MS.gene049025:CDS
AGTTTCAAAGGATCCTCGTA+TGG 0.625984 4.1:-3458319 MS.gene049025:CDS
TCCCCAAAGGGTGTTATCGT+AGG 0.628321 4.1:+3458422 None:intergenic
CTGGATCTACGGCTGTAATG+GGG 0.631123 4.1:+3458755 None:intergenic
ACAAAAGCAAGTTCCATACG+AGG 0.723375 4.1:+3458306 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATTTTAGATTAATTAAGT+AGG + chr4.1:3458379-3458398 None:intergenic 10.0%
!!! TTAATTAATCTAAAATTTTG+TGG - chr4.1:3458381-3458400 MS.gene049025:CDS 10.0%
!! AAATAACTTCTATGATTTAT+AGG + chr4.1:3458526-3458545 None:intergenic 15.0%
!! AAAATTTGATTATGCATACA+AGG - chr4.1:3458554-3458573 MS.gene049025:intron 20.0%
!! ACAATGTTAAATGAATATGT+TGG - chr4.1:3459068-3459087 MS.gene049025:intron 20.0%
!!! AAAGTATCACATATAGTTTA+TGG - chr4.1:3458892-3458911 MS.gene049025:intron 20.0%
! AACTTCTATGATTTATAGGT+TGG + chr4.1:3458522-3458541 None:intergenic 25.0%
! AAGGAAAAGAAAAACCATTA+TGG + chr4.1:3459412-3459431 None:intergenic 25.0%
! AGGAAAAGAAAAACCATTAT+GGG + chr4.1:3459411-3459430 None:intergenic 25.0%
! AGTCTTAAGAATAATTACAG+AGG + chr4.1:3458485-3458504 None:intergenic 25.0%
! ATGCTGACAAAAATAACTAT+TGG - chr4.1:3459482-3459501 MS.gene049025:intron 25.0%
! ATGTTATTGTAATTCTTCAG+TGG - chr4.1:3458682-3458701 MS.gene049025:CDS 25.0%
! CTTATCAAATTGGTTAAGAT+CGG - chr4.1:3459520-3459539 MS.gene049025:CDS 25.0%
! TCTAAAAGTTTATCAAGAAC+TGG + chr4.1:3459333-3459352 None:intergenic 25.0%
!! TAGTTCAAAAGACTTTTACT+TGG + chr4.1:3458925-3458944 None:intergenic 25.0%
AGGACTTCCATTCATAAAAA+AGG - chr4.1:3459263-3459282 MS.gene049025:CDS 30.0%
ATACAAACCTTTCCATCATA+TGG + chr4.1:3458835-3458854 None:intergenic 30.0%
CTAAACAGTTACAACAAACA+TGG + chr4.1:3458346-3458365 None:intergenic 30.0%
GCTAAAAAGACAATTGAAGT+AGG - chr4.1:3458766-3458785 MS.gene049025:CDS 30.0%
TAAATGTATGCAAAGAGAGA+GGG + chr4.1:3459156-3459175 None:intergenic 30.0%
TTTCAATGAAGCAGTTTCAA+AGG - chr4.1:3459645-3459664 MS.gene049025:intron 30.0%
TTTCAGAAACAAAGAGAGAA+TGG - chr4.1:3459605-3459624 MS.gene049025:intron 30.0%
! ATTTTTGTCAGCATCAACAA+AGG + chr4.1:3459476-3459495 None:intergenic 30.0%
! CTTTTGAACTAGAACAATGA+TGG - chr4.1:3458934-3458953 MS.gene049025:intron 30.0%
! TAACATTCCATATGATGGAA+AGG - chr4.1:3458825-3458844 MS.gene049025:intron 30.0%
!! GAACTTGCTTTTGTTTCAAT+AGG - chr4.1:3459679-3459698 MS.gene049025:CDS 30.0%
ACTATTGGAATTACCATGAG+AGG - chr4.1:3459497-3459516 MS.gene049025:intron 35.0%
ATCAAATTGGTTAAGATCGG+TGG - chr4.1:3459523-3459542 MS.gene049025:CDS 35.0%
ATCTCCACATATATCTTTCC+AGG - chr4.1:3459375-3459394 MS.gene049025:intron 35.0%
CTAAATGTATGCAAAGAGAG+AGG + chr4.1:3459157-3459176 None:intergenic 35.0%
CTTCCATTCATAAAAAAGGC+TGG - chr4.1:3459267-3459286 MS.gene049025:CDS 35.0%
CTTCTTCTCTACCAAATTGA+CGG + chr4.1:3459029-3459048 None:intergenic 35.0%
GACAGAAAAGCTTATGAGAT+AGG - chr4.1:3459243-3459262 MS.gene049025:CDS 35.0%
GCACTTAACATTCCATATGA+TGG - chr4.1:3458820-3458839 MS.gene049025:intron 35.0%
TAATTCTTCAGTGGCTTCAT+GGG - chr4.1:3458691-3458710 MS.gene049025:CDS 35.0%
TCAAATTGGTTAAGATCGGT+GGG - chr4.1:3459524-3459543 MS.gene049025:CDS 35.0%
! ATCTCATAAGCTTTTCTGTC+TGG + chr4.1:3459243-3459262 None:intergenic 35.0%
! ATTGAAGTAGGAGTTTTCAC+TGG - chr4.1:3458778-3458797 MS.gene049025:CDS 35.0%
!! TCAAAAGTACCTTCATTTGC+AGG + chr4.1:3459448-3459467 None:intergenic 35.0%
!!! ACACCAGCCTTTTTTATGAA+TGG + chr4.1:3459273-3459292 None:intergenic 35.0%
AAACCATTATGGGTCTGACT+TGG + chr4.1:3459401-3459420 None:intergenic 40.0%
ACAAAAGCAAGTTCCATACG+AGG + chr4.1:3459673-3459692 None:intergenic 40.0%
AGTTTCAAAGGATCCTCGTA+TGG - chr4.1:3459657-3459676 MS.gene049025:intron 40.0%
CCAATTTGATAAGCCTCTCA+TGG + chr4.1:3459513-3459532 None:intergenic 40.0%
CCATGAGAGGCTTATCAAAT+TGG - chr4.1:3459510-3459529 MS.gene049025:intron 40.0%
CGTCAATTTGGTAGAGAAGA+AGG - chr4.1:3459027-3459046 MS.gene049025:intron 40.0%
CTGCAAAATAACTGCATTGG+TGG + chr4.1:3458285-3458304 None:intergenic 40.0%
CTGTAGAAGCCAGTATGTAT+AGG + chr4.1:3458868-3458887 None:intergenic 40.0%
GAATCCAATCACCGTCAATT+TGG - chr4.1:3459015-3459034 MS.gene049025:intron 40.0%
GCTCTGCAAAATAACTGCAT+TGG + chr4.1:3458288-3458307 None:intergenic 40.0%
GTAATTCTTCAGTGGCTTCA+TGG - chr4.1:3458690-3458709 MS.gene049025:CDS 40.0%
TAGAAGCCAGTATGTATAGG+AGG + chr4.1:3458865-3458884 None:intergenic 40.0%
TCTACCAAATTGACGGTGAT+TGG + chr4.1:3459022-3459041 None:intergenic 40.0%
TGCTTTCCTCCTATACATAC+TGG - chr4.1:3458856-3458875 MS.gene049025:intron 40.0%
TTATCAAGAACTGGCAAAGC+TGG + chr4.1:3459324-3459343 None:intergenic 40.0%
TTGGCCTGGAAAGATATATG+TGG + chr4.1:3459382-3459401 None:intergenic 40.0%
! AGCTTTTCTGTCTGGATCTA+CGG + chr4.1:3459235-3459254 None:intergenic 40.0%
!! ATACATACTGGCTTCTACAG+TGG - chr4.1:3458868-3458887 MS.gene049025:intron 40.0%
!! GCTTTTGTTTCAATAGGCGA+CGG - chr4.1:3459685-3459704 MS.gene049025:CDS 40.0%
!! GTTTCTGAAACTACCGTTTC+TGG + chr4.1:3459595-3459614 None:intergenic 40.0%
AACTGTTGCCTTGCCAGAAA+CGG - chr4.1:3459579-3459598 MS.gene049025:intron 45.0%
TCTGGATCTACGGCTGTAAT+GGG + chr4.1:3459225-3459244 None:intergenic 45.0%
TGACTTCTTCAGTCAGCATC+AGG - chr4.1:3458642-3458661 MS.gene049025:CDS 45.0%
TGCCTACGATAACACCCTTT+GGG - chr4.1:3459552-3459571 MS.gene049025:CDS 45.0%
TTGATGCAGCCTGCAAATGA+AGG - chr4.1:3459436-3459455 MS.gene049025:intron 45.0%
TTGCCTACGATAACACCCTT+TGG - chr4.1:3459551-3459570 MS.gene049025:CDS 45.0%
!! GCAGTTATTTTGCAGAGCGA+GGG - chr4.1:3458290-3458309 MS.gene049025:CDS 45.0%
!! TGAAACTACCGTTTCTGGCA+AGG + chr4.1:3459590-3459609 None:intergenic 45.0%
!! TGCAGTTATTTTGCAGAGCG+AGG - chr4.1:3458289-3458308 MS.gene049025:CDS 45.0%
AGGCCAAGTCAGACCCATAA+TGG - chr4.1:3459395-3459414 MS.gene049025:intron 50.0%
CTGGATCTACGGCTGTAATG+GGG + chr4.1:3459224-3459243 None:intergenic 50.0%
GCCTACGATAACACCCTTTG+GGG - chr4.1:3459553-3459572 MS.gene049025:CDS 50.0%
GTCTGGATCTACGGCTGTAA+TGG + chr4.1:3459226-3459245 None:intergenic 50.0%
TGAGAGTCTCTGCTTCTCGT+GGG + chr4.1:3458433-3458452 None:intergenic 50.0%
TGCAGGCTGCATCAAGACAA+AGG + chr4.1:3459431-3459450 None:intergenic 50.0%
TGCTTCTCGTGGGTAAACAC+TGG + chr4.1:3458423-3458442 None:intergenic 50.0%
TTGAGAGTCTCTGCTTCTCG+TGG + chr4.1:3458434-3458453 None:intergenic 50.0%
! TACGATAACACCCTTTGGGG+AGG - chr4.1:3459556-3459575 MS.gene049025:CDS 50.0%
!! ATTATGGGTCTGACTTGGCC+TGG + chr4.1:3459396-3459415 None:intergenic 50.0%
!! TCCCCAAAGGGTGTTATCGT+AGG + chr4.1:3459557-3459576 None:intergenic 50.0%
ACCTGATGCGGGTCAGCTAA+TGG - chr4.1:3458720-3458739 MS.gene049025:CDS 55.0%
AGGCAACAGTTCCTCCCCAA+AGG + chr4.1:3459570-3459589 None:intergenic 55.0%
ATGGGCACTTCACCTGATGC+GGG - chr4.1:3458709-3458728 MS.gene049025:CDS 55.0%
CATGGGCACTTCACCTGATG+CGG - chr4.1:3458708-3458727 MS.gene049025:CDS 55.0%
GGCAACAGTTCCTCCCCAAA+GGG + chr4.1:3459569-3459588 None:intergenic 55.0%
GCAGAGCGAGGGCTTACTCA+AGG - chr4.1:3458301-3458320 MS.gene049025:CDS 60.0%
GCCATTAGCTGACCCGCATC+AGG + chr4.1:3458724-3458743 None:intergenic 60.0%
! GCGACGGACTCACTATCTGC+AGG - chr4.1:3459701-3459720 MS.gene049025:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 3458263 3459735 3458263 ID=MS.gene049025
chr4.1 mRNA 3458263 3459735 3458263 ID=MS.gene049025.t1;Parent=MS.gene049025
chr4.1 exon 3459676 3459735 3459676 ID=MS.gene049025.t1.exon1;Parent=MS.gene049025.t1
chr4.1 CDS 3459676 3459735 3459676 ID=cds.MS.gene049025.t1;Parent=MS.gene049025.t1
chr4.1 exon 3459512 3459591 3459512 ID=MS.gene049025.t1.exon2;Parent=MS.gene049025.t1
chr4.1 CDS 3459512 3459591 3459512 ID=cds.MS.gene049025.t1;Parent=MS.gene049025.t1
chr4.1 exon 3459152 3459296 3459152 ID=MS.gene049025.t1.exon3;Parent=MS.gene049025.t1
chr4.1 CDS 3459152 3459296 3459152 ID=cds.MS.gene049025.t1;Parent=MS.gene049025.t1
chr4.1 exon 3458642 3458821 3458642 ID=MS.gene049025.t1.exon4;Parent=MS.gene049025.t1
chr4.1 CDS 3458642 3458821 3458642 ID=cds.MS.gene049025.t1;Parent=MS.gene049025.t1
chr4.1 exon 3458263 3458553 3458263 ID=MS.gene049025.t1.exon5;Parent=MS.gene049025.t1
chr4.1 CDS 3458263 3458553 3458263 ID=cds.MS.gene049025.t1;Parent=MS.gene049025.t1
Gene Sequence

>MS.gene049025

ATGGACAAAATATCAGCTTCCACCAATGCAGTTATTTTGCAGAGCGAGGGCTTACTCAAGGTTTACATACACATCTCTTGCCATGTTTGTTGTAACTGTTTAGATTGATTTTTCCTACTTAATTAATCTAAAATTTTGTGGCAGTATATACTAGAAACCAGTGTTTACCCACGAGAAGCAGAGACTCTCAAAGAGCTGAGAAATGCAACAGCAAATCATCCTCTGTAATTATTCTTAAGACTGCTATACTATACATCCAACCTATAAATCATAGAAGTTATTTAACATAACAAAATTTGATTATGCATACAAGGATTTTTCAAACAAAAATAGTATGATATTATTATTATTATTATTATTATTTTATTCTTGTTGAACTTGACTTCTTCAGTCAGCATCAGGAAACTTTTTCTTATGTAATGTTATTGTAATTCTTCAGTGGCTTCATGGGCACTTCACCTGATGCGGGTCAGCTAATGGCTTTGCTCTTGAAACTTCTAAATGCTAAAAAGACAATTGAAGTAGGAGTTTTCACTGGATACTCTCTTCTACTCACAGCACTTAACATTCCATATGATGGAAAGGTTTGTATCTGCTTTCCTCCTATACATACTGGCTTCTACAGTGGAAAAGTATCACATATAGTTTATGGTAGATCACCAAGTAAAAGTCTTTTGAACTAGAACAATGATGGATGTTGCTCTTTGTAAGTAGAAGCTCATGATATTATGCATTCTCAAGACTGAGACGATGAATCCAATCACCGTCAATTTGGTAGAGAAGAAGGAACAAAAAAACTATTTGAACAATGTTAAATGAATATGTTGGTCATCGTAAGAGTTTTGAACAATTTCAATGCATAAGATGCTTAATTCTCACGCTAGCAATCACCCTCTCTCTTTGCATACATTTAGATAATAACGTCGCAAGTATGTGTCTATTATTTTTATGTTACTAGCCCCATTACAGCCGTAGATCCAGACAGAAAAGCTTATGAGATAGGACTTCCATTCATAAAAAAGGCTGGTGTCTTACACAAGATTGACTACATAGAGTCTCCAGCTTTGCCAGTTCTTGATAAACTTTTAGAAGATGTAAGCAACTTACTTAACATCTCCACATATATCTTTCCAGGCCAAGTCAGACCCATAATGGTTTTTCTTTTCCTTTGTCTTGATGCAGCCTGCAAATGAAGGTACTTTTGACTTTGCCTTTGTTGATGCTGACAAAAATAACTATTGGAATTACCATGAGAGGCTTATCAAATTGGTTAAGATCGGTGGGATTGTTGCCTACGATAACACCCTTTGGGGAGGAACTGTTGCCTTGCCAGAAACGGTAGTTTCAGAAACAAAGAGAGAATGGAGAAGATGTACACTTGATTTCAATGAAGCAGTTTCAAAGGATCCTCGTATGGAACTTGCTTTTGTTTCAATAGGCGACGGACTCACTATCTGCAGGCGCATTTGCTGA

Protein sequence

>MS.gene049025.t1

MDKISASTNAVILQSEGLLKYILETSVYPREAETLKELRNATANHPLGFMGTSPDAGQLMALLLKLLNAKKTIEVGVFTGYSLLLTALNIPYDGKIITSQVCVYYFYVTSPITAVDPDRKAYEIGLPFIKKAGVLHKIDYIESPALPVLDKLLEDPANEGTFDFAFVDADKNNYWNYHERLIKLVKIGGIVAYDNTLWGGTVALPETVVSETKREWRRCTLDFNEAVSKDPRMELAFVSIGDGLTICRRIC