AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene028389

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene028389.t1 MTR_4g094368 97.817 229 5 0 1 229 1 229 4.11e-166 457
MS.gene028389.t1 MTR_4g094925 54.386 228 97 3 3 227 17 240 3.04e-83 248
MS.gene028389.t1 MTR_4g085590 52.212 226 105 2 3 227 21 244 1.50e-81 244
MS.gene028389.t1 MTR_4g094815 52.655 226 104 3 3 227 22 245 4.76e-81 243
MS.gene028389.t1 MTR_8g021225 54.018 224 100 2 5 227 11 232 2.62e-80 240
MS.gene028389.t1 MTR_4g094812 55.310 226 98 2 3 227 20 243 6.18e-80 239
MS.gene028389.t1 MTR_4g094898 55.752 226 97 3 3 227 22 245 8.57e-80 239
MS.gene028389.t1 MTR_4g094925 55.135 185 82 1 44 227 25 209 1.10e-70 215
MS.gene028389.t1 MTR_8g101900 52.232 224 103 3 5 227 12 232 4.30e-70 214
MS.gene028389.t1 MTR_4g094898 58.152 184 76 1 45 227 1 184 4.92e-68 207
MS.gene028389.t1 MTR_6g091680 49.784 231 106 5 4 227 8 235 2.05e-66 205
MS.gene028389.t1 MTR_2g070410 40.000 220 121 4 8 227 88 296 1.29e-46 156
MS.gene028389.t1 MTR_2g070410 41.954 174 96 2 8 181 88 256 2.09e-38 134
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene028389.t1 AT1G67980 61.674 227 84 2 3 229 8 231 9.34e-97 282
MS.gene028389.t1 AT1G24735 60.444 225 88 1 5 229 16 239 1.28e-94 277
MS.gene028389.t1 AT1G67990 56.828 227 96 2 3 229 8 232 1.57e-90 266
MS.gene028389.t1 AT1G24735 49.275 276 88 2 5 229 16 290 1.25e-84 253
MS.gene028389.t1 AT4G34050 50.442 226 109 2 3 227 33 256 1.10e-78 237
MS.gene028389.t1 AT4G34050 50.442 226 109 2 3 227 60 283 3.90e-78 236
MS.gene028389.t1 AT4G26220 50.655 229 109 4 2 229 6 231 3.80e-75 227
MS.gene028389.t1 AT1G24735 59.259 189 76 1 41 229 7 194 1.50e-74 224
MS.gene028389.t1 AT3G61990 41.176 221 114 4 8 227 83 288 2.39e-53 173
MS.gene028389.t1 AT1G67980 51.515 165 77 2 65 229 2 163 6.91e-49 158
MS.gene028389.t1 AT3G62000 38.914 221 122 5 8 227 68 276 1.65e-46 155
MS.gene028389.t1 AT3G62000 37.736 212 119 5 8 218 68 267 3.88e-39 138
MS.gene028389.t1 AT4G34050 44.628 121 66 1 108 227 25 145 3.37e-32 115

Find 38 sgRNAs with CRISPR-Local

Find 156 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAACAACAATTCAGAAGTA+TGG 0.226874 4.2:+18471813 MS.gene028389:CDS
TTCAATTGTTCGTGCTCTTT+TGG 0.241069 4.2:-18471780 None:intergenic
TTTCATCACTTTCAGGTAAT+AGG 0.252449 4.2:+18472197 MS.gene028389:intron
GAATCAACAATCCTTTCTAT+TGG 0.264328 4.2:+18473949 MS.gene028389:CDS
AATCAACAATCCTTTCTATT+GGG 0.280127 4.2:+18473950 MS.gene028389:CDS
ATCCACAGTGATGCATTATC+AGG 0.304543 4.2:+18472299 MS.gene028389:CDS
TCACGAGCTGTTGTTGAAAT+TGG 0.308284 4.2:+18473777 MS.gene028389:CDS
TCAGGTAATAGGAATTGATA+TGG 0.352155 4.2:+18472208 MS.gene028389:intron
CAAAACTCCAGCACTTCTTC+AGG 0.360798 4.2:+18471634 MS.gene028389:CDS
TTGCATATGATAACACATTA+TGG 0.375536 4.2:+18473818 MS.gene028389:CDS
ATCATTTGATTATGCATTTG+TGG 0.388543 4.2:+18473726 MS.gene028389:CDS
AGGAAATGGAAGATACAATA+AGG 0.400536 4.2:+18473869 MS.gene028389:CDS
TTTGAGGATGTTCTTGGCCA+TGG 0.424703 4.2:-18471615 None:intergenic
AACTTCTGCCTTCTGAATGA+AGG 0.443554 4.2:-18472258 None:intergenic
TGCTCTTCCTTCTGATGGCA+AGG 0.457076 4.2:+18472108 MS.gene028389:CDS
GCTCTTGCTCTTCCTTCTGA+TGG 0.470052 4.2:+18472103 MS.gene028389:CDS
TTAGAAATTGGAGTCTTCAC+TGG 0.475666 4.2:+18472061 MS.gene028389:CDS
ATTATTACCTGAAGAAGTGC+TGG 0.476254 4.2:-18471641 None:intergenic
GAGTATAATGAATGTGCCTT+TGG 0.477530 4.2:+18471985 MS.gene028389:CDS
GTTGTTGAAATTGGTGAAGA+AGG 0.485943 4.2:+18473786 MS.gene028389:CDS
CATGTCTGAGGATGAGGAAA+TGG 0.492122 4.2:+18473855 MS.gene028389:CDS
TGTAGCCATGTCTGAGGATG+AGG 0.495939 4.2:+18473849 MS.gene028389:CDS
TATGATAACACATTATGGTC+TGG 0.512907 4.2:+18473823 MS.gene028389:CDS
AAACAACAATTCAGAAGTAT+GGG 0.513999 4.2:+18471814 MS.gene028389:CDS
GATAAAGAAGCATATGAGAC+AGG 0.521908 4.2:+18472230 MS.gene028389:CDS
ATCAACAATCCTTTCTATTG+GGG 0.546246 4.2:+18473951 MS.gene028389:CDS
TTGAAATTGGTGAAGAAGGG+TGG 0.553175 4.2:+18473790 MS.gene028389:CDS
AATGATACCTTGCCATCAGA+AGG 0.554204 4.2:-18472115 None:intergenic
ACTTCTGCCTTCTGAATGAA+GGG 0.557570 4.2:-18472257 None:intergenic
ATGAATTGTGCTTCATCCAA+AGG 0.563739 4.2:-18472001 None:intergenic
TTACACCGTCCCCAATAGAA+AGG 0.565363 4.2:-18473960 None:intergenic
TTGTTGAAATTGGTGAAGAA+GGG 0.567331 4.2:+18473787 MS.gene028389:CDS
AGGATTGCCCTTCATTCAGA+AGG 0.568251 4.2:+18472250 MS.gene028389:CDS
CATTTCCTCATCCTCAGACA+TGG 0.568293 4.2:-18473854 None:intergenic
TGCATTTGTGGATGCTGACA+AGG 0.577388 4.2:+18473738 MS.gene028389:CDS
ACAATCCTTTCTATTGGGGA+CGG 0.594828 4.2:+18473955 MS.gene028389:CDS
TGGTTCTGTAGCCATGTCTG+AGG 0.624517 4.2:+18473843 MS.gene028389:CDS
TGCCTGATAATGCATCACTG+TGG 0.800721 4.2:-18472301 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATAGAAAATCATATATTTTT+TGG + chr4.2:18473091-18473110 MS.gene028389:intron 10.0%
!! AATAAGATAATCATTCAATT+TGG - chr4.2:18472461-18472480 None:intergenic 15.0%
!! TTCAATTTCTTAAGTATATT+TGG + chr4.2:18473614-18473633 MS.gene028389:intron 15.0%
!!! ACATTGATTTTAATATTTGT+TGG + chr4.2:18471947-18471966 MS.gene028389:intron 15.0%
!!! CATTGATTTTAATATTTGTT+GGG + chr4.2:18471948-18471967 MS.gene028389:intron 15.0%
!!! CTTATAATAATAGTTCTTTT+CGG + chr4.2:18472514-18472533 MS.gene028389:intron 15.0%
!! ATAATCATTCAATTTGGATT+GGG - chr4.2:18472455-18472474 None:intergenic 20.0%
!! GAATTAACGAATAAATTAAC+TGG - chr4.2:18472942-18472961 None:intergenic 20.0%
!! TAATTAATATCTCTAGTAGA+AGG - chr4.2:18471709-18471728 None:intergenic 20.0%
!! TTAAGAAAACTCAATTTATC+TGG - chr4.2:18471685-18471704 None:intergenic 20.0%
! AAGGAAAATCTTAGTAAAAG+AGG + chr4.2:18473015-18473034 MS.gene028389:intron 25.0%
! AATCAACAATCCTTTCTATT+GGG + chr4.2:18473950-18473969 MS.gene028389:CDS 25.0%
! AGGAAAATCTTAGTAAAAGA+GGG + chr4.2:18473016-18473035 MS.gene028389:intron 25.0%
! ATCATTTGATTATGCATTTG+TGG + chr4.2:18473726-18473745 MS.gene028389:CDS 25.0%
! GAATAAATTTCATCACTTTC+AGG + chr4.2:18472190-18472209 MS.gene028389:intron 25.0%
! GATAATCATTCAATTTGGAT+TGG - chr4.2:18472456-18472475 None:intergenic 25.0%
! GCGAAAAAAACATTAGAAAT+TGG + chr4.2:18472049-18472068 MS.gene028389:CDS 25.0%
! GTTTCGAAAAGAAAACTTAT+GGG + chr4.2:18473586-18473605 MS.gene028389:intron 25.0%
! TCGTTTAGACAATGAATTAA+AGG + chr4.2:18473665-18473684 MS.gene028389:intron 25.0%
! TGTTTCGAAAAGAAAACTTA+TGG + chr4.2:18473585-18473604 MS.gene028389:intron 25.0%
! TTGCATATGATAACACATTA+TGG + chr4.2:18473818-18473837 MS.gene028389:CDS 25.0%
!! AAACAACAATTCAGAAGTAT+GGG + chr4.2:18471814-18471833 MS.gene028389:CDS 25.0%
!! ATAATAATAGTTCTTTTCGG+TGG + chr4.2:18472517-18472536 MS.gene028389:intron 25.0%
!! TCAATTGCAACTTTTCTTTT+TGG - chr4.2:18473895-18473914 None:intergenic 25.0%
!! TTACTAAGATTTTCCTTAGT+AGG - chr4.2:18473012-18473031 None:intergenic 25.0%
!!! ACTAGTACTGACATTTTTTA+TGG - chr4.2:18472964-18472983 None:intergenic 25.0%
!!! ATTCATTTTCTGCTTTGTTA+GGG + chr4.2:18473156-18473175 MS.gene028389:intron 25.0%
!!! ATTTTAATATTTGTTGGGTG+TGG + chr4.2:18471953-18471972 MS.gene028389:intron 25.0%
!!! TATTCATTTTCTGCTTTGTT+AGG + chr4.2:18473155-18473174 MS.gene028389:intron 25.0%
!!! TGTTTTAAATTGTATCATCG+TGG + chr4.2:18473339-18473358 MS.gene028389:intron 25.0%
!!! TTTTGGTTGTCCAATTTTAT+CGG + chr4.2:18473108-18473127 MS.gene028389:intron 25.0%
AAAACAATTCCATGAATTGC+AGG + chr4.2:18473690-18473709 MS.gene028389:intron 30.0%
AAACAATTCCATGAATTGCA+GGG + chr4.2:18473691-18473710 MS.gene028389:intron 30.0%
AGGAAATGGAAGATACAATA+AGG + chr4.2:18473869-18473888 MS.gene028389:CDS 30.0%
ATCAACAATCCTTTCTATTG+GGG + chr4.2:18473951-18473970 MS.gene028389:CDS 30.0%
ATCCAAACAACCGATAAAAT+TGG - chr4.2:18473121-18473140 None:intergenic 30.0%
GAAAACTTAAGGAATGTGAT+TGG + chr4.2:18472597-18472616 MS.gene028389:intron 30.0%
GAAACAACAATTCAGAAGTA+TGG + chr4.2:18471813-18471832 MS.gene028389:CDS 30.0%
GAATCAACAATCCTTTCTAT+TGG + chr4.2:18473949-18473968 MS.gene028389:CDS 30.0%
GGAAAATCTTAGTAAAAGAG+GGG + chr4.2:18473017-18473036 MS.gene028389:intron 30.0%
TATGATAACACATTATGGTC+TGG + chr4.2:18473823-18473842 MS.gene028389:CDS 30.0%
TCAGGTAATAGGAATTGATA+TGG + chr4.2:18472208-18472227 MS.gene028389:intron 30.0%
TTAACTGGTTATTCACTTTC+AGG - chr4.2:18472927-18472946 None:intergenic 30.0%
TTGTTGAAATTGGTGAAGAA+GGG + chr4.2:18473787-18473806 MS.gene028389:CDS 30.0%
! AACTTGTTTCAAGAACATAC+TGG - chr4.2:18471755-18471774 None:intergenic 30.0%
! GAGAAAAAGGTTGTTTCAAT+GGG + chr4.2:18472756-18472775 MS.gene028389:intron 30.0%
! TTTCATCACTTTCAGGTAAT+AGG + chr4.2:18472197-18472216 MS.gene028389:intron 30.0%
!! GTTGGTTTGATGAAAACTTA+AGG + chr4.2:18472586-18472605 MS.gene028389:intron 30.0%
!! TTTTTACGTGTTACTAGTAC+TGG + chr4.2:18473264-18473283 MS.gene028389:intron 30.0%
!!! AAATGTCAGTACTAGTTTTG+AGG + chr4.2:18472968-18472987 MS.gene028389:intron 30.0%
!!! TTTTGAGGCTTTTCCAAAAT+AGG + chr4.2:18472983-18473002 MS.gene028389:intron 30.0%
AAACGAATTAGATGCTCTAG+AGG - chr4.2:18473651-18473670 None:intergenic 35.0%
AAATCGTCTTCATTGATCCA+TGG - chr4.2:18472654-18472673 None:intergenic 35.0%
AACTCTTCTTCTCTATGTGT+TGG + chr4.2:18473048-18473067 MS.gene028389:intron 35.0%
AATCTTCTTTGCCTAACCAA+TGG - chr4.2:18472887-18472906 None:intergenic 35.0%
ATGAATTGTGCTTCATCCAA+AGG - chr4.2:18472004-18472023 None:intergenic 35.0%
CAAACCTGAGAAATAGAAAC+CGG - chr4.2:18473228-18473247 None:intergenic 35.0%
CCAAAATAGGATTCCTACTA+AGG + chr4.2:18472996-18473015 MS.gene028389:intron 35.0%
GAGTATAATGAATGTGCCTT+TGG + chr4.2:18471985-18472004 MS.gene028389:CDS 35.0%
GATATGATTGTTGAACGAGT+TGG + chr4.2:18472734-18472753 MS.gene028389:intron 35.0%
GTTCAACAATCATATCTGCT+TGG - chr4.2:18472730-18472749 None:intergenic 35.0%
GTTGTTGAAATTGGTGAAGA+AGG + chr4.2:18473786-18473805 MS.gene028389:CDS 35.0%
TAAGGAATGTGATTGGAGAT+GGG + chr4.2:18472604-18472623 MS.gene028389:intron 35.0%
TAGCAATATCGTCTTTGTGT+CGG + chr4.2:18473376-18473395 MS.gene028389:intron 35.0%
TATCATCCTTTATGTAGCAG+TGG + chr4.2:18473311-18473330 MS.gene028389:intron 35.0%
TTAAGGAATGTGATTGGAGA+TGG + chr4.2:18472603-18472622 MS.gene028389:intron 35.0%
TTAGAAATTGGAGTCTTCAC+TGG + chr4.2:18472061-18472080 MS.gene028389:CDS 35.0%
TTGGTTAGGCAAAGAAGATT+CGG + chr4.2:18472887-18472906 MS.gene028389:intron 35.0%
! AAAAAGGTTGTTTCAATGGG+AGG + chr4.2:18472759-18472778 MS.gene028389:intron 35.0%
! AATCAATAGCGAGGTATCAT+AGG + chr4.2:18472550-18472569 MS.gene028389:intron 35.0%
! ATAATAGTTCTTTTCGGTGG+AGG + chr4.2:18472520-18472539 MS.gene028389:intron 35.0%
! ATGGGAAAGAGACGAATTTT+TGG + chr4.2:18472622-18472641 MS.gene028389:intron 35.0%
! ATTATTACCTGAAGAAGTGC+TGG - chr4.2:18471644-18471663 None:intergenic 35.0%
! CCTTAGTAGGAATCCTATTT+TGG - chr4.2:18472999-18473018 None:intergenic 35.0%
! CTCAATTTATCTGGTTTCAG+TGG - chr4.2:18471676-18471695 None:intergenic 35.0%
! GATAAAGAAGCATATGAGAC+AGG + chr4.2:18472230-18472249 MS.gene028389:CDS 35.0%
! GGAGAAAAAGGTTGTTTCAA+TGG + chr4.2:18472755-18472774 MS.gene028389:intron 35.0%
! TAATAGTTCTTTTCGGTGGA+GGG + chr4.2:18472521-18472540 MS.gene028389:intron 35.0%
! TTCAATTGTTCGTGCTCTTT+TGG - chr4.2:18471783-18471802 None:intergenic 35.0%
! TTCTATTTCTCAGGTTTGGA+TGG + chr4.2:18473230-18473249 MS.gene028389:intron 35.0%
!! ATCATCGTGGCTTTATTGTA+TGG + chr4.2:18473352-18473371 MS.gene028389:intron 35.0%
!! GTCCAATTTTATCGGTTGTT+TGG + chr4.2:18473116-18473135 MS.gene028389:intron 35.0%
!! TCATCGTGGCTTTATTGTAT+GGG + chr4.2:18473353-18473372 MS.gene028389:intron 35.0%
AACTTCTGCCTTCTGAATGA+AGG - chr4.2:18472261-18472280 None:intergenic 40.0%
AATGATACCTTGCCATCAGA+AGG - chr4.2:18472118-18472137 None:intergenic 40.0%
ACAATCCTTTCTATTGGGGA+CGG + chr4.2:18473955-18473974 MS.gene028389:CDS 40.0%
ACAGGAAGATGTTTGGAAGT+GGG + chr4.2:18472830-18472849 MS.gene028389:intron 40.0%
ACTTCTGCCTTCTGAATGAA+GGG - chr4.2:18472260-18472279 None:intergenic 40.0%
AGAGCAAGAGAAATGCTCAA+AGG - chr4.2:18473406-18473425 None:intergenic 40.0%
ATCCACAGTGATGCATTATC+AGG + chr4.2:18472299-18472318 MS.gene028389:CDS 40.0%
ATCGGTTGTTTGGATTGAGT+CGG + chr4.2:18473126-18473145 MS.gene028389:intron 40.0%
CAAGATCTACAAGGTCTCAA+CGG - chr4.2:18473507-18473526 None:intergenic 40.0%
TCACGAGCTGTTGTTGAAAT+TGG + chr4.2:18473777-18473796 MS.gene028389:CDS 40.0%
TCTTCTTCTCTATGTGTTGG+TGG + chr4.2:18473051-18473070 MS.gene028389:intron 40.0%
TTAACACTCATCTTGTGCTG+TGG + chr4.2:18473471-18473490 MS.gene028389:intron 40.0%
TTGAAATTGGTGAAGAAGGG+TGG + chr4.2:18473790-18473809 MS.gene028389:CDS 40.0%
! CGGTTTCTATTTCTCAGGTT+TGG + chr4.2:18473226-18473245 MS.gene028389:intron 40.0%
! GTTGAACGAGTTGGAGAAAA+AGG + chr4.2:18472743-18472762 MS.gene028389:intron 40.0%
! TTGTTCGTGCTCTTTTGGAT+AGG - chr4.2:18471778-18471797 None:intergenic 40.0%
!! CGAATTTTTGGATAGAGCCA+TGG + chr4.2:18472634-18472653 MS.gene028389:intron 40.0%
!! TACTAGTACTGGTTCACTCT+TGG + chr4.2:18473275-18473294 MS.gene028389:intron 40.0%
!!! TGGAGTTTTGAGGATGTTCT+TGG - chr4.2:18471624-18471643 None:intergenic 40.0%
AGACCTTGTAGATCTTGCTC+GGG + chr4.2:18473510-18473529 MS.gene028389:intron 45.0%
AGAGGCGAGTTTCTCAGTTA+AGG + chr4.2:18472854-18472873 MS.gene028389:intron 45.0%
AGGATTGCCCTTCATTCAGA+AGG + chr4.2:18472250-18472269 MS.gene028389:CDS 45.0%
AGGCAAAGAAGATTCGGAAG+TGG + chr4.2:18472893-18472912 MS.gene028389:intron 45.0%
AGTCCTGTCTGATTGTGCAT+TGG + chr4.2:18473182-18473201 MS.gene028389:intron 45.0%
CAAAACTCCAGCACTTCTTC+AGG + chr4.2:18471634-18471653 MS.gene028389:CDS 45.0%
CAAAGAAGATTCGGAAGTGG+TGG + chr4.2:18472896-18472915 MS.gene028389:intron 45.0%
CAGGAAGATGTTTGGAAGTG+GGG + chr4.2:18472831-18472850 MS.gene028389:intron 45.0%
CAGTTAAGGCAGCTTACCAT+TGG + chr4.2:18472868-18472887 MS.gene028389:intron 45.0%
CATGTCTGAGGATGAGGAAA+TGG + chr4.2:18473855-18473874 MS.gene028389:CDS 45.0%
CATGTTTGCCCTGCAATTCA+TGG - chr4.2:18473702-18473721 None:intergenic 45.0%
CATTTCCTCATCCTCAGACA+TGG - chr4.2:18473857-18473876 None:intergenic 45.0%
GACAGGAAGATGTTTGGAAG+TGG + chr4.2:18472829-18472848 MS.gene028389:intron 45.0%
GAGACCTTGTAGATCTTGCT+CGG + chr4.2:18473509-18473528 MS.gene028389:intron 45.0%
GGGAATCGAAATCAATAGCG+AGG + chr4.2:18472541-18472560 MS.gene028389:intron 45.0%
GTCCTGTCTGATTGTGCATT+GGG + chr4.2:18473183-18473202 MS.gene028389:intron 45.0%
TAACCCGAGCAAGATCTACA+AGG - chr4.2:18473516-18473535 None:intergenic 45.0%
TCCTGTCTGATTGTGCATTG+GGG + chr4.2:18473184-18473203 MS.gene028389:intron 45.0%
TGCATTTGTGGATGCTGACA+AGG + chr4.2:18473738-18473757 MS.gene028389:CDS 45.0%
TTACACCGTCCCCAATAGAA+AGG - chr4.2:18473963-18473982 None:intergenic 45.0%
! AAGGCAGCTTACCATTGGTT+AGG + chr4.2:18472873-18472892 MS.gene028389:intron 45.0%
! AAGGTTGTTTCAATGGGAGG+AGG + chr4.2:18472762-18472781 MS.gene028389:intron 45.0%
! ATATTTGTTGGGTGTGGCAC+TGG + chr4.2:18471959-18471978 MS.gene028389:intron 45.0%
! TGCCTGATAATGCATCACTG+TGG - chr4.2:18472304-18472323 None:intergenic 45.0%
!! CTCTATGTGTTGGTGGATTG+TGG + chr4.2:18473058-18473077 MS.gene028389:intron 45.0%
!! TGTGCCGGTTTCTATTTCTC+AGG + chr4.2:18473221-18473240 MS.gene028389:intron 45.0%
!!! GAAGAAGTGCTGGAGTTTTG+AGG - chr4.2:18471634-18471653 None:intergenic 45.0%
ACCCCAATGCACAATCAGAC+AGG - chr4.2:18473188-18473207 None:intergenic 50.0%
AGATGTTTGGAAGTGGGGAG+AGG + chr4.2:18472836-18472855 MS.gene028389:intron 50.0%
ATCGCGCCACTGCTACATAA+AGG - chr4.2:18473320-18473339 None:intergenic 50.0%
CTTGGAGTTGAGAAGGTGGA+GGG + chr4.2:18472798-18472817 MS.gene028389:intron 50.0%
GCTCTTGCTCTTCCTTCTGA+TGG + chr4.2:18472103-18472122 MS.gene028389:CDS 50.0%
GGACAGGACAGGAAGATGTT+TGG + chr4.2:18472823-18472842 MS.gene028389:intron 50.0%
GTTTCAATGGGAGGAGGAGA+TGG + chr4.2:18472768-18472787 MS.gene028389:intron 50.0%
TCTGATTGTGCATTGGGGTC+TGG + chr4.2:18473189-18473208 MS.gene028389:intron 50.0%
TCTGGTGCTTGGAGTTGAGA+AGG + chr4.2:18472791-18472810 MS.gene028389:intron 50.0%
TGCTCTTCCTTCTGATGGCA+AGG + chr4.2:18472108-18472127 MS.gene028389:CDS 50.0%
TGTAGCCATGTCTGAGGATG+AGG + chr4.2:18473849-18473868 MS.gene028389:CDS 50.0%
! AAGATTCGGAAGTGGTGGAG+TGG + chr4.2:18472901-18472920 MS.gene028389:intron 50.0%
! ATAGCGAGGTATCATAGGCG+TGG + chr4.2:18472555-18472574 MS.gene028389:intron 50.0%
! TGGTTCTGTAGCCATGTCTG+AGG + chr4.2:18473843-18473862 MS.gene028389:CDS 50.0%
AATGGGAGGAGGAGATGGTC+TGG + chr4.2:18472773-18472792 MS.gene028389:intron 55.0%
GAGTTGAGAAGGTGGAGGGA+GGG + chr4.2:18472802-18472821 MS.gene028389:intron 55.0%
GCTTGGAGTTGAGAAGGTGG+AGG + chr4.2:18472797-18472816 MS.gene028389:intron 55.0%
GGTGCTTGGAGTTGAGAAGG+TGG + chr4.2:18472794-18472813 MS.gene028389:intron 55.0%
! TGTGCATTGGGGTCTGGTTC+TGG + chr4.2:18473195-18473214 MS.gene028389:intron 55.0%
ATAGGCGTGGAGGAGCGAGT+TGG + chr4.2:18472568-18472587 MS.gene028389:intron 60.0%
GGAGGAGATGGTCTGGTGCT+TGG + chr4.2:18472780-18472799 MS.gene028389:intron 60.0%
GGAGTTGAGAAGGTGGAGGG+AGG + chr4.2:18472801-18472820 MS.gene028389:intron 60.0%
! GCGAGGTATCATAGGCGTGG+AGG + chr4.2:18472558-18472577 MS.gene028389:intron 60.0%
!! GTCTGGTTCTGGCTCTGTGC+CGG + chr4.2:18473206-18473225 MS.gene028389:intron 60.0%
!! TTGTTGGGTGTGGCACTGGC+AGG + chr4.2:18471963-18471982 MS.gene028389:intron 60.0%
GAGAAGGTGGAGGGAGGGAC+AGG + chr4.2:18472807-18472826 MS.gene028389:intron 65.0%
GGTGGAGGGAGGGACAGGAC+AGG + chr4.2:18472812-18472831 MS.gene028389:intron 70.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 18471617 18474002 18471617 ID=MS.gene028389
chr4.2 mRNA 18471617 18474002 18471617 ID=MS.gene028389.t1;Parent=MS.gene028389
chr4.2 exon 18471617 18471655 18471617 ID=MS.gene028389.t1.exon1;Parent=MS.gene028389.t1
chr4.2 CDS 18471617 18471655 18471617 ID=cds.MS.gene028389.t1;Parent=MS.gene028389.t1
chr4.2 exon 18471756 18471841 18471756 ID=MS.gene028389.t1.exon2;Parent=MS.gene028389.t1
chr4.2 CDS 18471756 18471841 18471756 ID=cds.MS.gene028389.t1;Parent=MS.gene028389.t1
chr4.2 exon 18471985 18472129 18471985 ID=MS.gene028389.t1.exon3;Parent=MS.gene028389.t1
chr4.2 CDS 18471985 18472129 18471985 ID=cds.MS.gene028389.t1;Parent=MS.gene028389.t1
chr4.2 exon 18472212 18472340 18472212 ID=MS.gene028389.t1.exon4;Parent=MS.gene028389.t1
chr4.2 CDS 18472212 18472340 18472212 ID=cds.MS.gene028389.t1;Parent=MS.gene028389.t1
chr4.2 exon 18473712 18474002 18473712 ID=MS.gene028389.t1.exon5;Parent=MS.gene028389.t1
chr4.2 CDS 18473712 18474002 18473712 ID=cds.MS.gene028389.t1;Parent=MS.gene028389.t1
Gene Sequence

>MS.gene028389

ATGGCCAAGAACATCCTCAAAACTCCAGCACTTCTTCAGGTAATAATTAACTAATTCCACTGAAACCAGATAAATTGAGTTTTCTTAAACCTTCTACTAGAGATATTAATTAATTTTTGTCATCTTGTTGTTTAACCAGTATGTTCTTGAAACAAGTTCCTATCCAAAAGAGCACGAACAATTGAAACAACTTAGAGAAACAACAATTCAGAAGTATGGGAAATTGTAAGTTACACAACATAATACAATACGATCGATATAATCAAAGCAATTTATAATTTTAATTATAATGATTAAATATAATTAGTTGTATAAAATTAATTTGATCTTACATTGATTTTAATATTTGTTGGGTGTGGCACTGGCAGGAGTATAATGAATGTGCCTTTGGATGAAGCACAATTCATATCTATGCTTCTTAAAATTATGAATGCGAAAAAAACATTAGAAATTGGAGTCTTCACTGGTTATTCACTTCTTGCTACAGCTCTTGCTCTTCCTTCTGATGGCAAGGTATCATTTTAGTGTAAATCTTACATTTGATGATGATGAATAATTTAAATGAAATGTAACGAATAAATTTCATCACTTTCAGGTAATAGGAATTGATATGGATAAAGAAGCATATGAGACAGGATTGCCCTTCATTCAGAAGGCAGAAGTTGAGCACAAAATTGAATTTATCCACAGTGATGCATTATCAGGCATCATTAGTCTTATTGATGTAAGTGTTTTTTTTTCTCTCTCATTGATTTGAATTTTTGATCTGTTCGAAGCTTTTACAAATATGAGTAATTATCTGTGAATTAATTAAAGATATATTTATATAGTTGTTCCCAATCCAAATTGAATGATTATCTTATTGTAAAAAAACTTAAGTCATAATTGAATGATTATCTTATAATAATAGTTCTTTTCGGTGGAGGGAATCGAAATCAATAGCGAGGTATCATAGGCGTGGAGGAGCGAGTTGGTTTGATGAAAACTTAAGGAATGTGATTGGAGATGGGAAAGAGACGAATTTTTGGATAGAGCCATGGATCAATGAAGACGATTTGCGTCATCTCTTTAGTAGAGTTTATATGATATATCACTTGATAAAGATAAATGCCAAGCAGATATGATTGTTGAACGAGTTGGAGAAAAAGGTTGTTTCAATGGGAGGAGGAGATGGTCTGGTGCTTGGAGTTGAGAAGGTGGAGGGAGGGACAGGACAGGAAGATGTTTGGAAGTGGGGAGAGGCGAGTTTCTCAGTTAAGGCAGCTTACCATTGGTTAGGCAAAGAAGATTCGGAAGTGGTGGAGTGGCCTGAAAGTGAATAACCAGTTAATTTATTCGTTAATTCCATAAAAAATGTCAGTACTAGTTTTGAGGCTTTTCCAAAATAGGATTCCTACTAAGGAAAATCTTAGTAAAAGAGGGGTGAATCTAAACTCTTCTTCTCTATGTGTTGGTGGATTGTGGCAGAGTTGAAATAGAAAATCATATATTTTTTGGTTGTCCAATTTTATCGGTTGTTTGGATTGAGTCGGCTGTTGTATTCATTTTCTGCTTTGTTAGGGTCTAGTCCTGTCTGATTGTGCATTGGGGTCTGGTTCTGGCTCTGTGCCGGTTTCTATTTCTCAGGTTTGGATGGCTGTTGCAGTGTTTTTACGTGTTACTAGTACTGGTTCACTCTTGGAACTCACTGAATGTATCATCCTTTATGTAGCAGTGGCGCGATGTTTTAAATTGTATCATCGTGGCTTTATTGTATGGGTAGCAATATCGTCTTTGTGTCGGTTGTCCTTTGAGCATTTCTCTTGCTCTAGCTTTATCATGCGACTGTCAAGTGTCACTTTGATCTGAATCTTTTTAACACTCATCTTGTGCTGTGGCTGTATGTATCCGTTGAGACCTTGTAGATCTTGCTCGGGTTAGCGCTACTTGTGCTTAATTATGAATCTGTCTATATTAATAATATTTTGCTGTTTCGAAAAGAAAACTTATGGGTTTAATTCAATTTCTTAAGTATATTTGGTTTCACTTTAACCTCTAGAGCATCTAATTCGTTTAGACAATGAATTAAAGGAAAAAACAATTCCATGAATTGCAGGGCAAACATGAAGAATCATTTGATTATGCATTTGTGGATGCTGACAAGGAAAACTTCACAAAGTATCACGAGCTGTTGTTGAAATTGGTGAAGAAGGGTGGAATAATTGCATATGATAACACATTATGGTCTGGTTCTGTAGCCATGTCTGAGGATGAGGAAATGGAAGATACAATAAGGCCAAAAAGAAAAGTTGCAATTGAATTCAACAACTTTATAGCAAATGATACTCGCATTGAATCAACAATCCTTTCTATTGGGGACGGTGTAACTCTCTGCAGAGTTCTCTAA
Protein sequence

>MS.gene028389.t1

MAKNILKTPALLQYVLETSSYPKEHEQLKQLRETTIQKYGKLSIMNVPLDEAQFISMLLKIMNAKKTLEIGVFTGYSLLATALALPSDGKVIGIDMDKEAYETGLPFIQKAEVEHKIEFIHSDALSGIISLIDGKHEESFDYAFVDADKENFTKYHELLLKLVKKGGIIAYDNTLWSGSVAMSEDEEMEDTIRPKRKVAIEFNNFIANDTRIESTILSIGDGVTLCRVL