Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77183.t1 | XP_013464148.1 | 100 | 81 | 0 | 0 | 1 | 81 | 1 | 81 | 9.10E-33 | 149.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77183.t1 | I3SDA0 | 100.0 | 81 | 0 | 0 | 1 | 81 | 1 | 81 | 6.6e-33 | 149.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049181 | MS.gene77183 | 0.823234 | 1.55E-53 | -1.69E-46 |
| MS.gene050363 | MS.gene77183 | 0.805855 | 1.09E-49 | -1.69E-46 |
| MS.gene050410 | MS.gene77183 | 0.806533 | 7.86E-50 | -1.69E-46 |
| MS.gene050883 | MS.gene77183 | 0.805411 | 1.35E-49 | -1.69E-46 |
| MS.gene051959 | MS.gene77183 | 0.801855 | 7.39E-49 | -1.69E-46 |
| MS.gene051960 | MS.gene77183 | 0.805849 | 1.09E-49 | -1.69E-46 |
| MS.gene052053 | MS.gene77183 | 0.832989 | 6.90E-56 | -1.69E-46 |
| MS.gene052091 | MS.gene77183 | 0.831259 | 1.85E-55 | -1.69E-46 |
| MS.gene053826 | MS.gene77183 | 0.812287 | 4.57E-51 | -1.69E-46 |
| MS.gene053932 | MS.gene77183 | 0.810598 | 1.06E-50 | -1.69E-46 |
| MS.gene055150 | MS.gene77183 | 0.818811 | 1.61E-52 | -1.69E-46 |
| MS.gene055549 | MS.gene77183 | 0.890592 | 8.57E-74 | -1.69E-46 |
| MS.gene055550 | MS.gene77183 | 0.895512 | 8.93E-76 | -1.69E-46 |
| MS.gene055551 | MS.gene77183 | 0.860085 | 2.64E-63 | -1.69E-46 |
| MS.gene056311 | MS.gene77183 | 0.808564 | 2.91E-50 | -1.69E-46 |
| MS.gene056365 | MS.gene77183 | 0.820165 | 7.92E-53 | -1.69E-46 |
| MS.gene056530 | MS.gene77183 | 0.840483 | 8.46E-58 | -1.69E-46 |
| MS.gene058493 | MS.gene77183 | 0.814813 | 1.27E-51 | -1.69E-46 |
| MS.gene058494 | MS.gene77183 | 0.821584 | 3.74E-53 | -1.69E-46 |
| MS.gene05884 | MS.gene77183 | 0.80334 | 3.65E-49 | -1.69E-46 |
| MS.gene060964 | MS.gene77183 | 0.805617 | 1.22E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77183.t1 | MTR_2g061780 | 100.000 | 81 | 0 | 0 | 1 | 81 | 1 | 81 | 1.36e-53 | 161 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77183.t1 | AT2G31945 | 60.938 | 64 | 23 | 1 | 1 | 62 | 1 | 64 | 1.78e-20 | 78.2 |
| MS.gene77183.t1 | AT1G05575 | 56.000 | 75 | 31 | 1 | 1 | 73 | 1 | 75 | 2.14e-17 | 70.1 |
Find 20 sgRNAs with CRISPR-Local
Find 20 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTTTGATCTCTTGATGTTCT+TGG | 0.211211 | 2.4:-31695268 | None:intergenic |
| GAAGGAGAGGGTTCTCCTTC+AGG | 0.307513 | 2.4:-31695354 | None:intergenic |
| TTGATGAATTTGAAAGGATA+AGG | 0.383924 | 2.4:-31695236 | None:intergenic |
| CTCTGAGTTGCTTGTGATTG+AGG | 0.403222 | 2.4:-31695387 | None:intergenic |
| GATTGAGGACTCATTGTAGA+AGG | 0.403857 | 2.4:-31695372 | None:intergenic |
| CGTGAGTATTCGTCGGATGT+TGG | 0.440469 | 2.4:+31695311 | MS.gene77183:CDS |
| CAAAAGATGGATAGTAGCTT+AGG | 0.451025 | 2.4:+31695162 | None:intergenic |
| CTTAGGTGACATGTTATTGA+AGG | 0.471923 | 2.4:+31695179 | MS.gene77183:CDS |
| GTTCGTCCTAGTCCAAGCTT+TGG | 0.507181 | 2.4:+31695209 | MS.gene77183:CDS |
| GGATAAGGTACACCAAAGCT+TGG | 0.548918 | 2.4:-31695221 | None:intergenic |
| CGCGTTCCGTGAGTATTCGT+CGG | 0.549054 | 2.4:+31695304 | MS.gene77183:CDS |
| ATATGTTTGATGAATTTGAA+AGG | 0.553082 | 2.4:-31695242 | None:intergenic |
| AGGTGACATGTTATTGAAGG+TGG | 0.573319 | 2.4:+31695182 | MS.gene77183:CDS |
| CGAATACTCACGGAACGCGC+CGG | 0.585547 | 2.4:-31695300 | None:intergenic |
| GAGATCAAACAGTTTCAAGC+CGG | 0.604551 | 2.4:+31695281 | MS.gene77183:CDS |
| AGGACTCATTGTAGAAGGAG+AGG | 0.608887 | 2.4:-31695367 | None:intergenic |
| GGTACACCAAAGCTTGGACT+AGG | 0.611822 | 2.4:-31695215 | None:intergenic |
| GGACTCATTGTAGAAGGAGA+GGG | 0.615228 | 2.4:-31695366 | None:intergenic |
| GGAGAGGGTTCTCCTTCAGG+AGG | 0.644566 | 2.4:-31695351 | None:intergenic |
| CAACATCCGACGAATACTCA+CGG | 0.680704 | 2.4:-31695310 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATATGTTTGATGAATTTGAA+AGG | - | chr2.4:31695245-31695264 | None:intergenic | 20.0% |
| ! | TTGATGAATTTGAAAGGATA+AGG | - | chr2.4:31695239-31695258 | None:intergenic | 25.0% |
| CTTAGGTGACATGTTATTGA+AGG | + | chr2.4:31695179-31695198 | MS.gene77183:CDS | 35.0% | |
| GTTTGATCTCTTGATGTTCT+TGG | - | chr2.4:31695271-31695290 | None:intergenic | 35.0% | |
| AGGTGACATGTTATTGAAGG+TGG | + | chr2.4:31695182-31695201 | MS.gene77183:CDS | 40.0% | |
| GAGATCAAACAGTTTCAAGC+CGG | + | chr2.4:31695281-31695300 | MS.gene77183:CDS | 40.0% | |
| GATTGAGGACTCATTGTAGA+AGG | - | chr2.4:31695375-31695394 | None:intergenic | 40.0% | |
| AGGACTCATTGTAGAAGGAG+AGG | - | chr2.4:31695370-31695389 | None:intergenic | 45.0% | |
| CAACATCCGACGAATACTCA+CGG | - | chr2.4:31695313-31695332 | None:intergenic | 45.0% | |
| CTCTGAGTTGCTTGTGATTG+AGG | - | chr2.4:31695390-31695409 | None:intergenic | 45.0% | |
| GGACTCATTGTAGAAGGAGA+GGG | - | chr2.4:31695369-31695388 | None:intergenic | 45.0% | |
| GGATAAGGTACACCAAAGCT+TGG | - | chr2.4:31695224-31695243 | None:intergenic | 45.0% | |
| ! | CTCTCTGATTTTCCTCCTGA+AGG | + | chr2.4:31695339-31695358 | MS.gene77183:CDS | 45.0% |
| CGTGAGTATTCGTCGGATGT+TGG | + | chr2.4:31695311-31695330 | MS.gene77183:CDS | 50.0% | |
| GGTACACCAAAGCTTGGACT+AGG | - | chr2.4:31695218-31695237 | None:intergenic | 50.0% | |
| GTTCGTCCTAGTCCAAGCTT+TGG | + | chr2.4:31695209-31695228 | MS.gene77183:CDS | 50.0% | |
| CGCGTTCCGTGAGTATTCGT+CGG | + | chr2.4:31695304-31695323 | MS.gene77183:CDS | 55.0% | |
| GAAGGAGAGGGTTCTCCTTC+AGG | - | chr2.4:31695357-31695376 | None:intergenic | 55.0% | |
| CGAATACTCACGGAACGCGC+CGG | - | chr2.4:31695303-31695322 | None:intergenic | 60.0% | |
| GGAGAGGGTTCTCCTTCAGG+AGG | - | chr2.4:31695354-31695373 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.4 | gene | 31695168 | 31695413 | 31695168 | ID=MS.gene77183 |
| chr2.4 | mRNA | 31695168 | 31695413 | 31695168 | ID=MS.gene77183.t1;Parent=MS.gene77183 |
| chr2.4 | exon | 31695168 | 31695413 | 31695168 | ID=MS.gene77183.t1.exon1;Parent=MS.gene77183.t1 |
| chr2.4 | CDS | 31695168 | 31695413 | 31695168 | ID=cds.MS.gene77183.t1;Parent=MS.gene77183.t1 |
| Gene Sequence |
| Protein sequence |
