AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene052234


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene052234.t1 MTR_3g087770 99.342 152 1 0 1 152 1 152 2.94e-112 315
MS.gene052234.t1 MTR_8g091420 81.935 155 23 3 1 152 1 153 1.30e-79 232
MS.gene052234.t1 MTR_1g030630 56.376 149 61 3 5 152 2 147 6.03e-56 172
MS.gene052234.t1 MTR_3g073670 59.091 154 56 3 1 152 1 149 1.57e-55 171
MS.gene052234.t1 MTR_5g022620 55.769 156 61 4 5 152 2 157 1.94e-53 166
MS.gene052234.t1 MTR_3g117890 53.750 160 57 5 5 152 2 156 2.32e-52 163
MS.gene052234.t1 MTR_4g073040 54.194 155 64 4 5 152 2 156 7.15e-52 162
MS.gene052234.t1 MTR_6g003990 50.649 154 70 3 5 152 2 155 2.63e-51 160
MS.gene052234.t1 MTR_5g025150 52.027 148 67 1 5 152 2 145 6.33e-51 159
MS.gene052234.t1 MTR_7g083060 57.364 129 53 2 25 152 19 146 2.42e-47 150
MS.gene052234.t1 MTR_6g003990 53.390 118 52 2 38 152 1 118 3.17e-42 136
MS.gene052234.t1 MTR_5g055020 47.967 123 64 0 30 152 25 147 2.47e-40 132
MS.gene052234.t1 MTR_6g026890 39.456 147 77 4 17 152 3 148 2.77e-28 102
MS.gene052234.t1 MTR_1g078090 41.045 134 69 3 29 152 52 185 1.23e-27 101
MS.gene052234.t1 MTR_2g091235 35.135 148 66 5 29 152 1 142 6.19e-22 85.9
MS.gene052234.t1 MTR_6g045203 39.706 136 67 4 32 152 5 140 3.75e-18 75.9
MS.gene052234.t1 MTR_1g082650 53.731 67 31 0 28 94 8 74 4.78e-15 71.6
MS.gene052234.t1 MTR_1g107565 50.746 67 33 0 28 94 11 77 7.88e-15 70.5
MS.gene052234.t1 MTR_2g022250 47.761 67 35 0 28 94 8 74 7.29e-14 68.2
MS.gene052234.t1 MTR_1g092670 49.231 65 33 0 30 94 4 68 1.06e-13 62.8
MS.gene052234.t1 MTR_7g013660 47.692 65 34 0 30 94 4 68 1.22e-13 63.9
MS.gene052234.t1 MTR_7g108560 49.231 65 33 0 30 94 22 86 1.32e-13 66.6
MS.gene052234.t1 MTR_4g094238 38.824 85 49 2 29 113 1 82 1.43e-13 62.8
MS.gene052234.t1 MTR_4g117140 46.269 67 36 0 28 94 8 74 3.02e-13 66.2
MS.gene052234.t1 MTR_4g117220 46.269 67 36 0 28 94 8 74 3.28e-13 66.2
MS.gene052234.t1 MTR_7g113110 48.387 62 32 0 34 95 37 98 1.10e-12 64.3
MS.gene052234.t1 MTR_3g067750 46.875 64 34 0 30 93 12 75 5.09e-12 61.2
MS.gene052234.t1 MTR_4g119820 46.970 66 35 0 30 95 74 139 1.42e-11 61.2
MS.gene052234.t1 MTR_1g083310 44.776 67 36 1 30 95 15 81 2.83e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene052234.t1 AT4G39700 70.886 158 40 3 1 152 1 158 3.63e-72 214
MS.gene052234.t1 AT1G22990 56.579 152 61 3 5 152 2 152 8.07e-58 177
MS.gene052234.t1 AT1G71050 59.355 155 57 3 1 152 1 152 7.31e-56 172
MS.gene052234.t1 AT1G22990 55.034 149 63 3 5 152 2 147 7.24e-55 169
MS.gene052234.t1 AT4G38580 54.902 153 63 3 5 152 2 153 1.23e-54 169
MS.gene052234.t1 AT4G38580 54.902 153 63 3 5 152 2 153 1.23e-54 169
MS.gene052234.t1 AT4G08570 55.844 154 62 3 1 152 1 150 4.89e-54 167
MS.gene052234.t1 AT5G17450 50.667 150 70 4 5 152 2 149 5.73e-46 147
MS.gene052234.t1 AT5G66110 51.007 149 63 4 10 152 3 147 8.82e-45 144
MS.gene052234.t1 AT5G66110 51.007 149 63 4 10 152 3 147 8.82e-45 144
MS.gene052234.t1 AT4G35060 47.403 154 73 4 5 152 2 153 2.01e-43 140
MS.gene052234.t1 AT5G66110 52.893 121 51 3 38 152 1 121 5.30e-38 126
MS.gene052234.t1 AT5G17450 55.556 99 41 3 56 152 8 105 3.30e-31 108
MS.gene052234.t1 AT1G06330 38.961 154 60 5 29 152 1 150 1.29e-26 98.2
MS.gene052234.t1 AT3G56891 39.216 153 63 6 29 152 15 166 6.23e-25 94.4
MS.gene052234.t1 AT2G18196 36.301 146 81 5 19 152 33 178 2.68e-24 92.8
MS.gene052234.t1 AT4G10465 38.849 139 75 3 24 152 45 183 1.85e-23 90.9
MS.gene052234.t1 AT3G56891 38.194 144 59 6 38 152 1 143 2.47e-22 87.0
MS.gene052234.t1 AT1G29100 37.584 149 61 4 31 152 4 147 3.04e-21 84.3
MS.gene052234.t1 AT1G29100 36.986 146 60 4 34 152 1 141 5.50e-20 80.9
MS.gene052234.t1 AT2G18196 35.200 125 71 4 38 152 1 125 4.19e-19 78.2
MS.gene052234.t1 AT3G48970 38.686 137 69 4 31 152 4 140 7.49e-15 67.4
MS.gene052234.t1 AT3G48970 38.686 137 69 4 31 152 2 138 8.96e-15 67.4
MS.gene052234.t1 AT3G48970 38.686 137 69 4 31 152 2 138 8.96e-15 67.4
MS.gene052234.t1 AT1G66240 44.444 72 40 0 23 94 26 97 7.42e-14 63.9
MS.gene052234.t1 AT5G27690 48.387 62 32 0 34 95 34 95 1.47e-13 67.0
MS.gene052234.t1 AT3G06130 48.485 66 34 0 29 94 9 74 2.06e-13 66.6
MS.gene052234.t1 AT3G56240 50.000 66 33 0 30 95 3 68 2.82e-13 62.8
MS.gene052234.t1 AT3G56240 50.000 66 33 0 30 95 3 68 2.82e-13 62.8
MS.gene052234.t1 AT3G06130 47.761 67 35 0 28 94 8 74 3.89e-13 65.9
MS.gene052234.t1 AT5G19090 46.269 67 36 0 28 94 8 74 3.56e-12 63.2
MS.gene052234.t1 AT5G19090 46.269 67 36 0 28 94 8 74 3.56e-12 63.2
MS.gene052234.t1 AT5G19090 46.269 67 36 0 28 94 8 74 3.56e-12 63.2
MS.gene052234.t1 AT5G19090 46.269 67 36 0 28 94 8 74 4.52e-12 62.8
MS.gene052234.t1 AT5G19090 46.269 67 36 0 28 94 8 74 4.52e-12 62.8
MS.gene052234.t1 AT1G56210 49.231 65 33 0 34 98 44 108 5.48e-12 62.4
MS.gene052234.t1 AT1G23000 42.857 70 40 0 29 98 72 141 1.79e-11 61.2
MS.gene052234.t1 AT1G23000 42.857 70 40 0 29 98 12 81 1.97e-11 60.8
MS.gene052234.t1 AT5G37860 40.845 71 42 0 29 99 9 79 3.68e-11 59.7

Find 41 sgRNAs with CRISPR-Local

Find 54 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCCTATCATCATCTACATAA+TGG 0.292404 3.2:+74833768 None:intergenic
TACATAATGGAGCAGGCATT+TGG 0.304332 3.2:+74833781 None:intergenic
AGAGTACTTATCTGATCTAA+TGG 0.306737 3.2:-74834481 MS.gene052234:CDS
TAAAATCAGTGGAGATAAAC+AGG 0.372946 3.2:-74834047 MS.gene052234:CDS
TTGCGATGGTTGCGAACTTA+AGG 0.383976 3.2:-74834385 MS.gene052234:CDS
ACCATGTTGTATGGCACATA+AGG 0.433890 3.2:+74833934 None:intergenic
AGCAAAGTCAACAGGAAAGA+AGG 0.439458 3.2:-74833968 MS.gene052234:CDS
CAGGAAAGAAGGCAGAGATT+TGG 0.440709 3.2:-74833957 MS.gene052234:CDS
ACATAATGGAGCAGGCATTT+GGG 0.448683 3.2:+74833782 None:intergenic
GGGGTATGTAGAGGCAAATA+AGG 0.452605 3.2:-74834001 MS.gene052234:CDS
TCCTGGATATGTAAGGAGAT+TGG 0.456785 3.2:-74833869 MS.gene052234:CDS
TCCAATCTCCTTACATATCC+AGG 0.463407 3.2:+74833868 None:intergenic
CTAAAGAAAGCAAAGTCAAC+AGG 0.471841 3.2:-74833976 MS.gene052234:CDS
GAGTACTTATCTGATCTAAT+GGG 0.482086 3.2:-74834480 MS.gene052234:CDS
AAAGCCCTCTCTTCAATGAA+TGG 0.498175 3.2:-74834357 MS.gene052234:intron
ATTACAGACCGTAGAGTTGA+AGG 0.499504 3.2:-74834418 MS.gene052234:CDS
TATGACAAGAAGGCTCCTCC+TGG 0.508900 3.2:-74833886 MS.gene052234:CDS
CGTAGAGTTGAAGGTGAGGA+TGG 0.509056 3.2:-74834409 MS.gene052234:CDS
CTTACCATTCATTGAAGAGA+GGG 0.529075 3.2:+74834353 None:intergenic
ATATACACAGAAATGGGAGT+TGG 0.529253 3.2:-74834513 None:intergenic
AGGCTCCTCCTGGATATGTA+AGG 0.531905 3.2:-74833876 MS.gene052234:CDS
GGAGCCTTCTTGTCATAAGA+AGG 0.533318 3.2:+74833892 None:intergenic
GGATGAGCCACCATGTTGTA+TGG 0.533759 3.2:+74833925 None:intergenic
TCATAAGAAGGAGCAGCGTA+TGG 0.549598 3.2:+74833904 None:intergenic
TACACAGAAATGGGAGTTGG+AGG 0.552311 3.2:-74834510 None:intergenic
CATCATCTACATAATGGAGC+AGG 0.555456 3.2:+74833774 None:intergenic
TGCTCCTTCTTATGACAAGA+AGG 0.562724 3.2:-74833896 MS.gene052234:CDS
GTAAGGAGATTGGAGACCAC+TGG 0.572647 3.2:-74833859 MS.gene052234:CDS
TTATCTGATCTAATGGGCAG+TGG 0.573748 3.2:-74834474 MS.gene052234:CDS
CATCCTCACCTTCAACTCTA+CGG 0.585179 3.2:+74834410 None:intergenic
GACAGTAACGGGGTATGTAG+AGG 0.602619 3.2:-74834010 MS.gene052234:CDS
ACTTACCATTCATTGAAGAG+AGG 0.616934 3.2:+74834352 None:intergenic
CATCACTAAACATGGTGGTG+AGG 0.624282 3.2:+74833809 None:intergenic
GCCTTATGTGCCATACAACA+TGG 0.647674 3.2:-74833935 MS.gene052234:CDS
ATATGCTCTAACAGTTCCAG+TGG 0.649114 3.2:+74833843 None:intergenic
AAGGTGAGGATGGATTGCGA+TGG 0.658836 3.2:-74834399 MS.gene052234:CDS
CTAAACATGGTGGTGAGGTG+TGG 0.665051 3.2:+74833814 None:intergenic
AGACCGTAGAGTTGAAGGTG+AGG 0.688480 3.2:-74834413 MS.gene052234:CDS
TGATGCAGGAGTAAAATCAG+TGG 0.694945 3.2:-74834058 MS.gene052234:intron
TTATGTGCCATACAACATGG+TGG 0.751150 3.2:-74833932 MS.gene052234:CDS
AATCTCCTTACATATCCAGG+AGG 0.766746 3.2:+74833871 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGAGAAAGATAAATTAATAG+AGG + chr3.2:74833964-74833983 None:intergenic 20.0%
!! GAGAAAGATAAATTAATAGA+GGG + chr3.2:74833963-74833982 None:intergenic 20.0%
! TCAAATTATAATTAGTGCGT+CGG - chr3.2:74834074-74834093 MS.gene052234:intron 25.0%
ACAACAAAAAGTGACAGTAA+CGG - chr3.2:74834259-74834278 MS.gene052234:intron 30.0%
GAGTACTTATCTGATCTAAT+GGG - chr3.2:74833801-74833820 MS.gene052234:CDS 30.0%
TAAAATCAGTGGAGATAAAC+AGG - chr3.2:74834234-74834253 MS.gene052234:intron 30.0%
TATCTTTCTCTATATAGCAC+CGG - chr3.2:74833973-74833992 MS.gene052234:CDS 30.0%
! AGAGTACTTATCTGATCTAA+TGG - chr3.2:74833800-74833819 MS.gene052234:CDS 30.0%
! TCTTCTTCTTGATTTTGTGA+TGG + chr3.2:74833839-74833858 None:intergenic 30.0%
!!! TTTTTTTTTTGTGGTGATGC+AGG - chr3.2:74834209-74834228 MS.gene052234:intron 30.0%
AACAAAAAGTGACAGTAACG+GGG - chr3.2:74834261-74834280 MS.gene052234:intron 35.0%
ACTTACCATTCATTGAAGAG+AGG + chr3.2:74833932-74833951 None:intergenic 35.0%
CAACAAAAAGTGACAGTAAC+GGG - chr3.2:74834260-74834279 MS.gene052234:intron 35.0%
CTAAAGAAAGCAAAGTCAAC+AGG - chr3.2:74834305-74834324 MS.gene052234:intron 35.0%
CTTACCATTCATTGAAGAGA+GGG + chr3.2:74833931-74833950 None:intergenic 35.0%
GCTTGCTTGCATTATATGTA+TGG - chr3.2:74834130-74834149 MS.gene052234:intron 35.0%
! GTTTTCATCACTAAACATGG+TGG + chr3.2:74834480-74834499 None:intergenic 35.0%
! TCTTCTTGATTTTGTGATGG+TGG + chr3.2:74833836-74833855 None:intergenic 35.0%
! TGGGTTTTCATCACTAAACA+TGG + chr3.2:74834483-74834502 None:intergenic 35.0%
AAAGCCCTCTCTTCAATGAA+TGG - chr3.2:74833924-74833943 MS.gene052234:CDS 40.0%
AATCTCCTTACATATCCAGG+AGG + chr3.2:74834413-74834432 None:intergenic 40.0%
ACCATGTTGTATGGCACATA+AGG + chr3.2:74834350-74834369 None:intergenic 40.0%
ACGTCATCAATATGAAGTGC+CGG + chr3.2:74833995-74834014 None:intergenic 40.0%
AGCAAAGTCAACAGGAAAGA+AGG - chr3.2:74834313-74834332 MS.gene052234:intron 40.0%
ATATGCTCTAACAGTTCCAG+TGG + chr3.2:74834441-74834460 None:intergenic 40.0%
ATTACAGACCGTAGAGTTGA+AGG - chr3.2:74833863-74833882 MS.gene052234:CDS 40.0%
TCCAATCTCCTTACATATCC+AGG + chr3.2:74834416-74834435 None:intergenic 40.0%
TCCTGGATATGTAAGGAGAT+TGG - chr3.2:74834412-74834431 MS.gene052234:CDS 40.0%
TGATGCAGGAGTAAAATCAG+TGG - chr3.2:74834223-74834242 MS.gene052234:intron 40.0%
TGCTCCTTCTTATGACAAGA+AGG - chr3.2:74834385-74834404 MS.gene052234:CDS 40.0%
TTATGTGCCATACAACATGG+TGG - chr3.2:74834349-74834368 MS.gene052234:intron 40.0%
! ACATAATGGAGCAGGCATTT+GGG + chr3.2:74834502-74834521 None:intergenic 40.0%
! TACATAATGGAGCAGGCATT+TGG + chr3.2:74834503-74834522 None:intergenic 40.0%
! TTATCTGATCTAATGGGCAG+TGG - chr3.2:74833807-74833826 MS.gene052234:CDS 40.0%
CAGGAAAGAAGGCAGAGATT+TGG - chr3.2:74834324-74834343 MS.gene052234:intron 45.0%
CATCCTCACCTTCAACTCTA+CGG + chr3.2:74833874-74833893 None:intergenic 45.0%
GCCTTATGTGCCATACAACA+TGG - chr3.2:74834346-74834365 MS.gene052234:intron 45.0%
GGAGCCTTCTTGTCATAAGA+AGG + chr3.2:74834392-74834411 None:intergenic 45.0%
GGGGTATGTAGAGGCAAATA+AGG - chr3.2:74834280-74834299 MS.gene052234:intron 45.0%
TCATAAGAAGGAGCAGCGTA+TGG + chr3.2:74834380-74834399 None:intergenic 45.0%
TTGCGATGGTTGCGAACTTA+AGG - chr3.2:74833896-74833915 MS.gene052234:CDS 45.0%
! CATCACTAAACATGGTGGTG+AGG + chr3.2:74834475-74834494 None:intergenic 45.0%
!!! TTTTTTTTTTTTTTTTTTTG+TGG - chr3.2:74834200-74834219 MS.gene052234:intron 5.0%
AGACCGTAGAGTTGAAGGTG+AGG - chr3.2:74833868-74833887 MS.gene052234:CDS 50.0%
AGGCTCCTCCTGGATATGTA+AGG - chr3.2:74834405-74834424 MS.gene052234:CDS 50.0%
CGTAGAGTTGAAGGTGAGGA+TGG - chr3.2:74833872-74833891 MS.gene052234:CDS 50.0%
CGTCTGACATCACTGACACA+TGG - chr3.2:74834021-74834040 MS.gene052234:CDS 50.0%
GGATGAGCCACCATGTTGTA+TGG + chr3.2:74834359-74834378 None:intergenic 50.0%
GTAAGGAGATTGGAGACCAC+TGG - chr3.2:74834422-74834441 MS.gene052234:CDS 50.0%
GTGTCAGTGATGTCAGACGT+TGG + chr3.2:74834020-74834039 None:intergenic 50.0%
TATGACAAGAAGGCTCCTCC+TGG - chr3.2:74834395-74834414 MS.gene052234:CDS 50.0%
! CTAAACATGGTGGTGAGGTG+TGG + chr3.2:74834470-74834489 None:intergenic 50.0%
! GACAGTAACGGGGTATGTAG+AGG - chr3.2:74834271-74834290 MS.gene052234:intron 50.0%
!! AAGGTGAGGATGGATTGCGA+TGG - chr3.2:74833882-74833901 MS.gene052234:CDS 50.0%


Chromosome Type Strat End Strand Name
chr3.2 gene 74833780 74834523 74833780 ID=MS.gene052234
chr3.2 mRNA 74833780 74834523 74833780 ID=MS.gene052234.t1;Parent=MS.gene052234
chr3.2 exon 74834358 74834523 74834358 ID=MS.gene052234.t1.exon1;Parent=MS.gene052234.t1
chr3.2 CDS 74834358 74834523 74834358 ID=cds.MS.gene052234.t1;Parent=MS.gene052234.t1
chr3.2 exon 74833780 74834072 74833780 ID=MS.gene052234.t1.exon2;Parent=MS.gene052234.t1
chr3.2 CDS 74833780 74834072 74833780 ID=cds.MS.gene052234.t1;Parent=MS.gene052234.t1
Gene Sequence

>MS.gene052234

ATGGGAGTTGGAGGCACATTAGAGTACTTATCTGATCTAATGGGCAGTGGTCACCACCATCACAAAATCAAGAAGAAGAAGCAATTACAGACCGTAGAGTTGAAGGTGAGGATGGATTGCGATGGTTGCGAACTTAAGGTCAAGAAAGCCCTCTCTTCAATGAATGGTAAGTTACATTTTCCCTCTATTAATTTATCTTTCTCTATATAGCACCGGCACTTCATATTGATGACGTGTCCAACGTCTGACATCACTGACACATGGCTACATTTAAATATTAATCATTCTATTCTCTCAAATTATAATTAGTGCGTCGGTATAAATGTCGTATCTGATGTCAGTGTTTTATAGCTTGCTTGCATTATATGTATGGACATAGTATCAAGTACAATAGTACATGAAGCTAATGAAGTTGATTTTTTTTTTTTTTTTTTTTTTTGTGGTGATGCAGGAGTAAAATCAGTGGAGATAAACAGGAAACAACAAAAAGTGACAGTAACGGGGTATGTAGAGGCAAATAAGGTGCTAAAGAAAGCAAAGTCAACAGGAAAGAAGGCAGAGATTTGGCCTTATGTGCCATACAACATGGTGGCTCATCCATACGCTGCTCCTTCTTATGACAAGAAGGCTCCTCCTGGATATGTAAGGAGATTGGAGACCACTGGAACTGTTAGAGCATATGAAGAGCCACACCTCACCACCATGTTTAGTGATGAAAACCCAAATGCCTGCTCCATTATGTAG

Protein sequence

>MS.gene052234.t1

MGVGGTLEYLSDLMGSGHHHHKIKKKKQLQTVELKVRMDCDGCELKVKKALSSMNGVKSVEINRKQQKVTVTGYVEANKVLKKAKSTGKKAEIWPYVPYNMVAHPYAAPSYDKKAPPGYVRRLETTGTVRAYEEPHLTTMFSDENPNACSIM