Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065979.t1 | XP_003629868.1 | 96.9 | 675 | 20 | 1 | 1 | 675 | 1 | 674 | 0.00E+00 | 1241.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065979.t1 | Q9ZP55 | 56.1 | 672 | 283 | 4 | 3 | 668 | 2 | 667 | 5.2e-207 | 722.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065979.t1 | G7L7C1 | 96.9 | 675 | 20 | 1 | 1 | 675 | 1 | 674 | 0.0e+00 | 1241.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene065979 | 0.860133 | 2.55E-63 | -1.69E-46 |
| MS.gene049227 | MS.gene065979 | 0.802599 | 5.20E-49 | -1.69E-46 |
| MS.gene049672 | MS.gene065979 | 0.800103 | 1.69E-48 | -1.69E-46 |
| MS.gene049711 | MS.gene065979 | 0.809001 | 2.35E-50 | -1.69E-46 |
| MS.gene051018 | MS.gene065979 | 0.828157 | 1.05E-54 | -1.69E-46 |
| MS.gene051247 | MS.gene065979 | 0.816836 | 4.50E-52 | -1.69E-46 |
| MS.gene05157 | MS.gene065979 | 0.800223 | 1.59E-48 | -1.69E-46 |
| MS.gene051744 | MS.gene065979 | 0.819191 | 1.32E-52 | -1.69E-46 |
| MS.gene052234 | MS.gene065979 | 0.821279 | 4.39E-53 | -1.69E-46 |
| MS.gene052516 | MS.gene065979 | 0.812278 | 4.59E-51 | -1.69E-46 |
| MS.gene053144 | MS.gene065979 | 0.848713 | 5.14E-60 | -1.69E-46 |
| MS.gene05315 | MS.gene065979 | 0.809802 | 1.58E-50 | -1.69E-46 |
| MS.gene053226 | MS.gene065979 | 0.827385 | 1.61E-54 | -1.69E-46 |
| MS.gene054584 | MS.gene065979 | 0.849055 | 4.13E-60 | -1.69E-46 |
| MS.gene055465 | MS.gene065979 | 0.825262 | 5.16E-54 | -1.69E-46 |
| MS.gene055466 | MS.gene065979 | 0.829815 | 4.17E-55 | -1.69E-46 |
| MS.gene055468 | MS.gene065979 | 0.811683 | 6.19E-51 | -1.69E-46 |
| MS.gene055469 | MS.gene065979 | 0.805858 | 1.09E-49 | -1.69E-46 |
| MS.gene055513 | MS.gene065979 | 0.804346 | 2.26E-49 | -1.69E-46 |
| MS.gene055537 | MS.gene065979 | 0.85001 | 2.24E-60 | -1.69E-46 |
| MS.gene055580 | MS.gene065979 | -0.817789 | 2.75E-52 | -1.69E-46 |
| MS.gene056169 | MS.gene065979 | 0.800613 | 1.33E-48 | -1.69E-46 |
| MS.gene05686 | MS.gene065979 | 0.803347 | 3.64E-49 | -1.69E-46 |
| MS.gene057082 | MS.gene065979 | 0.808995 | 2.35E-50 | -1.69E-46 |
| MS.gene057132 | MS.gene065979 | 0.81628 | 6.00E-52 | -1.69E-46 |
| MS.gene057915 | MS.gene065979 | 0.821196 | 4.59E-53 | -1.69E-46 |
| MS.gene058010 | MS.gene065979 | 0.806125 | 9.57E-50 | -1.69E-46 |
| MS.gene058339 | MS.gene065979 | 0.810569 | 1.08E-50 | -1.69E-46 |
| MS.gene058668 | MS.gene065979 | 0.834001 | 3.86E-56 | -1.69E-46 |
| MS.gene058909 | MS.gene065979 | 0.833186 | 6.16E-56 | -1.69E-46 |
| MS.gene058912 | MS.gene065979 | 0.829707 | 4.43E-55 | -1.69E-46 |
| MS.gene059570 | MS.gene065979 | 0.833354 | 5.60E-56 | -1.69E-46 |
| MS.gene060962 | MS.gene065979 | 0.845681 | 3.49E-59 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene065979 | MS.gene32695 | PPI |
| MS.gene80193 | MS.gene065979 | PPI |
| MS.gene014901 | MS.gene065979 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065979.t1 | MTR_8g087770 | 96.889 | 675 | 20 | 1 | 1 | 675 | 1 | 674 | 0.0 | 1328 |
| MS.gene065979.t1 | MTR_8g087750 | 82.143 | 672 | 110 | 5 | 6 | 672 | 11 | 677 | 0.0 | 1109 |
| MS.gene065979.t1 | MTR_8g087763 | 76.991 | 226 | 51 | 1 | 374 | 599 | 4 | 228 | 7.01e-124 | 372 |
| MS.gene065979.t1 | MTR_4g093750 | 37.846 | 325 | 180 | 9 | 67 | 376 | 35 | 352 | 6.92e-42 | 162 |
| MS.gene065979.t1 | MTR_4g093750 | 39.726 | 292 | 154 | 9 | 67 | 343 | 35 | 319 | 2.55e-41 | 161 |
| MS.gene065979.t1 | MTR_2g009690 | 36.624 | 314 | 187 | 6 | 63 | 366 | 40 | 351 | 1.94e-40 | 159 |
| MS.gene065979.t1 | MTR_1g032550 | 35.689 | 283 | 162 | 7 | 69 | 337 | 35 | 311 | 1.08e-33 | 137 |
| MS.gene065979.t1 | MTR_8g105580 | 35.816 | 282 | 162 | 6 | 69 | 337 | 35 | 310 | 1.96e-33 | 135 |
| MS.gene065979.t1 | MTR_8g105580 | 35.816 | 282 | 162 | 6 | 69 | 337 | 35 | 310 | 2.00e-33 | 136 |
| MS.gene065979.t1 | MTR_5g009150 | 42.473 | 186 | 105 | 2 | 69 | 252 | 35 | 220 | 2.71e-32 | 132 |
| MS.gene065979.t1 | MTR_7g451010 | 35.616 | 292 | 164 | 8 | 69 | 346 | 40 | 321 | 3.95e-32 | 132 |
| MS.gene065979.t1 | MTR_8g099550 | 42.632 | 190 | 99 | 3 | 69 | 252 | 34 | 219 | 1.46e-31 | 130 |
| MS.gene065979.t1 | MTR_8g009510 | 34.810 | 316 | 182 | 9 | 69 | 369 | 40 | 346 | 2.01e-31 | 130 |
| MS.gene065979.t1 | MTR_8g027675 | 42.211 | 199 | 104 | 5 | 69 | 264 | 34 | 224 | 2.31e-31 | 130 |
| MS.gene065979.t1 | MTR_4g085860 | 75.641 | 78 | 18 | 1 | 206 | 282 | 2 | 79 | 7.19e-31 | 115 |
| MS.gene065979.t1 | MTR_4g098920 | 34.483 | 319 | 179 | 10 | 69 | 369 | 34 | 340 | 7.57e-31 | 128 |
| MS.gene065979.t1 | MTR_6g045107 | 74.359 | 78 | 19 | 1 | 206 | 282 | 2 | 79 | 6.21e-29 | 110 |
| MS.gene065979.t1 | MTR_8g463270 | 30.736 | 231 | 141 | 8 | 54 | 269 | 27 | 253 | 1.16e-16 | 84.7 |
| MS.gene065979.t1 | MTR_8g009510 | 30.488 | 246 | 151 | 6 | 136 | 369 | 2 | 239 | 4.87e-16 | 82.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065979.t1 | AT1G60500 | 56.101 | 672 | 283 | 4 | 3 | 668 | 2 | 667 | 0.0 | 768 |
| MS.gene065979.t1 | AT1G60530 | 72.069 | 290 | 73 | 1 | 18 | 307 | 19 | 300 | 3.94e-143 | 419 |
| MS.gene065979.t1 | AT4G33650 | 30.485 | 433 | 277 | 10 | 35 | 452 | 29 | 452 | 8.24e-40 | 157 |
| MS.gene065979.t1 | AT4G33650 | 30.485 | 433 | 277 | 10 | 35 | 452 | 29 | 452 | 9.50e-40 | 157 |
| MS.gene065979.t1 | AT2G14120 | 32.647 | 340 | 205 | 7 | 25 | 346 | 1 | 334 | 4.29e-39 | 154 |
| MS.gene065979.t1 | AT2G14120 | 32.022 | 356 | 217 | 8 | 25 | 362 | 1 | 349 | 5.60e-39 | 154 |
| MS.gene065979.t1 | AT2G14120 | 32.647 | 340 | 205 | 7 | 25 | 346 | 1 | 334 | 6.12e-39 | 154 |
| MS.gene065979.t1 | AT2G14120 | 32.022 | 356 | 217 | 8 | 25 | 362 | 1 | 349 | 6.29e-39 | 154 |
| MS.gene065979.t1 | AT1G14830 | 35.252 | 278 | 170 | 5 | 69 | 337 | 35 | 311 | 2.75e-35 | 142 |
| MS.gene065979.t1 | AT3G60190 | 33.226 | 310 | 195 | 6 | 69 | 369 | 40 | 346 | 1.58e-32 | 134 |
| MS.gene065979.t1 | AT5G42080 | 33.871 | 310 | 193 | 6 | 69 | 369 | 34 | 340 | 2.43e-32 | 130 |
| MS.gene065979.t1 | AT5G42080 | 33.871 | 310 | 193 | 6 | 69 | 369 | 34 | 340 | 6.22e-32 | 132 |
| MS.gene065979.t1 | AT5G42080 | 33.871 | 310 | 193 | 6 | 69 | 369 | 34 | 340 | 6.22e-32 | 132 |
| MS.gene065979.t1 | AT5G42080 | 33.871 | 310 | 193 | 6 | 69 | 369 | 34 | 340 | 6.48e-32 | 131 |
| MS.gene065979.t1 | AT2G44590 | 32.986 | 288 | 175 | 7 | 69 | 345 | 35 | 315 | 2.93e-31 | 129 |
| MS.gene065979.t1 | AT3G61760 | 41.667 | 204 | 109 | 5 | 69 | 268 | 34 | 231 | 8.11e-31 | 126 |
| MS.gene065979.t1 | AT3G61760 | 41.667 | 204 | 109 | 5 | 69 | 268 | 34 | 231 | 1.00e-30 | 128 |
| MS.gene065979.t1 | AT3G61760 | 41.667 | 204 | 109 | 5 | 69 | 268 | 34 | 231 | 1.29e-30 | 127 |
| MS.gene065979.t1 | AT2G44590 | 29.861 | 288 | 167 | 8 | 69 | 345 | 35 | 298 | 8.64e-22 | 100 |
| MS.gene065979.t1 | AT2G44590 | 29.861 | 288 | 167 | 8 | 69 | 345 | 35 | 298 | 9.90e-22 | 100 |
| MS.gene065979.t1 | AT1G53140 | 28.151 | 238 | 155 | 8 | 44 | 269 | 36 | 269 | 1.96e-15 | 80.9 |
Find 126 sgRNAs with CRISPR-Local
Find 130 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAGGAGTCTCCATCTATTTC+TGG | 0.166668 | 4.3:+13285582 | MS.gene065979:CDS |
| CATTGTTGGTATTCCTGTTT+TGG | 0.198486 | 4.3:+13284585 | MS.gene065979:CDS |
| GCAGTTCTTGGCATGAAATT+TGG | 0.208693 | 4.3:-13284982 | None:intergenic |
| TTAGTACTTGTGAGTCTATT+AGG | 0.242558 | 4.3:+13284335 | MS.gene065979:CDS |
| ATCAAGTTTGTTTGCCTTTG+TGG | 0.244719 | 4.3:-13283699 | None:intergenic |
| CTTGAGGAAGCTAAGTTAAT+TGG | 0.255531 | 4.3:+13284955 | MS.gene065979:CDS |
| GCCTGCCAATTTGACTTCAT+TGG | 0.264901 | 4.3:+13284717 | MS.gene065979:CDS |
| TGACAATGTGTGGAGCTATT+TGG | 0.298324 | 4.3:+13285065 | MS.gene065979:CDS |
| TCTGAGAAAGATTCTTCTAA+TGG | 0.298751 | 4.3:+13284789 | MS.gene065979:CDS |
| AATGTTAGAAAAGCAGTTCT+TGG | 0.299019 | 4.3:-13284994 | None:intergenic |
| ATCTGATCATAAATGTTATC+AGG | 0.304162 | 4.3:-13284238 | None:intergenic |
| TAACATTTATGATCAGATTA+AGG | 0.317964 | 4.3:+13284243 | MS.gene065979:CDS |
| CTTCCAACCATTGTTGTTGT+TGG | 0.322456 | 4.3:+13283881 | MS.gene065979:CDS |
| TGATGATGTTAACATTGGTT+TGG | 0.323376 | 4.3:+13284453 | MS.gene065979:CDS |
| TTCATTTCTTGCTTCTTCAT+AGG | 0.344245 | 4.3:-13284512 | None:intergenic |
| AGAGACTGTGGCCAACATTA+TGG | 0.346194 | 4.3:+13285653 | MS.gene065979:CDS |
| ATCAGCCAATGAAGTCAAAT+TGG | 0.365085 | 4.3:-13284722 | None:intergenic |
| CAAGGGTACCTTTGGTTATG+AGG | 0.366182 | 4.3:+13283981 | MS.gene065979:CDS |
| CAATGCATTTGTTTATCATC+AGG | 0.370755 | 4.3:-13284829 | None:intergenic |
| TGCAAACTATTACCAGTTGC+AGG | 0.374084 | 4.3:+13285122 | MS.gene065979:CDS |
| CTTACAATGGTTGATCTTCC+AGG | 0.382214 | 4.3:+13284187 | MS.gene065979:CDS |
| ACTTTCTCCAACAAACCTTC+AGG | 0.384947 | 4.3:-13284424 | None:intergenic |
| CTGAGAAAGATTCTTCTAAT+GGG | 0.390787 | 4.3:+13284790 | MS.gene065979:CDS |
| GATGATGTTAACATTGGTTT+GGG | 0.391771 | 4.3:+13284454 | MS.gene065979:CDS |
| GCTCTGGCTGTGGAAGTAAA+TGG | 0.394731 | 4.3:-13284012 | None:intergenic |
| TTCCATTAGGAAATCCTTTG+TGG | 0.394982 | 4.3:-13284920 | None:intergenic |
| TTGATCTTCTTAATTATCTC+AGG | 0.396848 | 4.3:-13284655 | None:intergenic |
| TCACAGATAGTTGATAAAAC+TGG | 0.398319 | 4.3:+13284361 | MS.gene065979:CDS |
| AGGCAAGATTAATTGCCTAT+TGG | 0.399341 | 4.3:+13285430 | MS.gene065979:CDS |
| AAGATTGACAAATCCATTGT+TGG | 0.402415 | 4.3:+13284571 | MS.gene065979:CDS |
| TCCGATCAATGTGTTGAATC+AGG | 0.406853 | 4.3:+13285395 | MS.gene065979:CDS |
| TTGAGGATAATGCTCTCTTC+AGG | 0.409426 | 4.3:-13284289 | None:intergenic |
| GAATCAGGCCTTTGACTTGA+AGG | 0.413968 | 4.3:+13285410 | MS.gene065979:CDS |
| ATGGACAGGATTGGAGTCTA+TGG | 0.415221 | 4.3:+13285672 | MS.gene065979:CDS |
| TTGATGCTCTTGAAAATCTA+AGG | 0.416842 | 4.3:+13283828 | MS.gene065979:CDS |
| TCCACAGCCAGAGCTTGTAT+TGG | 0.421767 | 4.3:+13284021 | MS.gene065979:CDS |
| TTGCAATCCGGAGTACTTGC+AGG | 0.423704 | 4.3:+13285248 | MS.gene065979:CDS |
| AATTTGTACAAGGGTACCTT+TGG | 0.424069 | 4.3:+13283973 | MS.gene065979:CDS |
| TCAAGTACTGTGAAGCTTGA+AGG | 0.424080 | 4.3:+13285336 | MS.gene065979:CDS |
| ACAGCTACTGAAGTGCTTGC+TGG | 0.424935 | 4.3:+13284100 | MS.gene065979:CDS |
| TGTCTCTTACCAGAAATAGA+TGG | 0.425419 | 4.3:-13285591 | None:intergenic |
| GAGCTTGTATTGGAGTATAA+TGG | 0.426836 | 4.3:+13284031 | MS.gene065979:CDS |
| GGACTACGATCTCTTCCATT+AGG | 0.434291 | 4.3:-13284933 | None:intergenic |
| TGTGATGCAAGCTGTTGATA+TGG | 0.436114 | 4.3:+13285206 | MS.gene065979:CDS |
| TAAATCACCTGAAGGTTTGT+TGG | 0.440077 | 4.3:+13284417 | MS.gene065979:CDS |
| TTTGGAAAGGGTTGTTATCT+CGG | 0.442091 | 4.3:+13285083 | MS.gene065979:CDS |
| CCTCAGCACAATCTTCCAAT+AGG | 0.443004 | 4.3:-13285445 | None:intergenic |
| TTCCACAAAGGATTTCCTAA+TGG | 0.449900 | 4.3:+13284918 | MS.gene065979:CDS |
| ACATTGTCAACAAAATTAGT+TGG | 0.454264 | 4.3:-13285051 | None:intergenic |
| GCATTGTACTGCTCGGTTAG+TGG | 0.455271 | 4.3:+13284846 | MS.gene065979:CDS |
| TCATTATAAGAAGATACAAT+TGG | 0.458646 | 4.3:-13283788 | None:intergenic |
| ATTGGAGTATAATGGCAAAC+AGG | 0.459418 | 4.3:+13284039 | MS.gene065979:CDS |
| TACGAGTAGCAACCTGCAAC+TGG | 0.461514 | 4.3:-13285134 | None:intergenic |
| ACAATTGGTGCAACAGATAG+AGG | 0.467988 | 4.3:-13283773 | None:intergenic |
| ACAAGCTTCTGAGCCAAAAC+AGG | 0.473524 | 4.3:-13284598 | None:intergenic |
| TGATACCAGCTAATGATTCA+AGG | 0.474056 | 4.3:-13283931 | None:intergenic |
| GTTGTTGGAGATCAATCATC+TGG | 0.485196 | 4.3:+13283896 | MS.gene065979:CDS |
| TGGAGTGTTTGAAATCCCTT+TGG | 0.490204 | 4.3:-13284128 | None:intergenic |
| TTATACTCCAATACAAGCTC+TGG | 0.492061 | 4.3:-13284028 | None:intergenic |
| GCCAATGAAGTCAAATTGGC+AGG | 0.500514 | 4.3:-13284718 | None:intergenic |
| GCGTCTCAACATTGCTAAAG+AGG | 0.501157 | 4.3:+13283850 | MS.gene065979:CDS |
| AGCTTGCATCACATGTTGAA+TGG | 0.501675 | 4.3:-13285196 | None:intergenic |
| CACGGATTACACTTGCAATC+CGG | 0.511102 | 4.3:+13285236 | MS.gene065979:CDS |
| CTGGAAGATCAACCATTGTA+AGG | 0.513573 | 4.3:-13284186 | None:intergenic |
| ATAACTCGTGTTCCTGTACA+TGG | 0.514492 | 4.3:+13284211 | MS.gene065979:CDS |
| TTTGTGTGAGAAATCGCATT+GGG | 0.515555 | 4.3:+13284482 | MS.gene065979:CDS |
| GTAAGAGACAAAGTTTAAGC+AGG | 0.516534 | 4.3:+13285604 | MS.gene065979:CDS |
| AATGCATTTGTTTATCATCA+GGG | 0.517273 | 4.3:-13284828 | None:intergenic |
| TCCAATACAAGCTCTGGCTG+TGG | 0.519083 | 4.3:-13284022 | None:intergenic |
| TCCAATCCTGTCCATAATGT+TGG | 0.522411 | 4.3:-13285664 | None:intergenic |
| AGTGTCCTTGAATCATTAGC+TGG | 0.523168 | 4.3:+13283926 | MS.gene065979:CDS |
| GGTGCAACAGATAGAGGTTG+AGG | 0.525331 | 4.3:-13283767 | None:intergenic |
| GTGCTTGCTGGAACTGCCAA+AGG | 0.525520 | 4.3:+13284112 | MS.gene065979:CDS |
| AGATCAACCATTGTAAGGTC+AGG | 0.531215 | 4.3:-13284181 | None:intergenic |
| TAATCTTGCCTTCAAGTCAA+AGG | 0.534214 | 4.3:-13285418 | None:intergenic |
| GCCAACATTATGGACAGGAT+TGG | 0.534644 | 4.3:+13285663 | MS.gene065979:CDS |
| GCCTGATTCAACACATTGAT+CGG | 0.536471 | 4.3:-13285396 | None:intergenic |
| AAGGAGATTTGTAATGAACT+TGG | 0.538785 | 4.3:+13285537 | MS.gene065979:CDS |
| ACTTTGATTGTGAAGAAGAA+TGG | 0.539337 | 4.3:+13284154 | MS.gene065979:CDS |
| ATCAGCTTGCCACGTGGACA+AGG | 0.540401 | 4.3:+13283950 | MS.gene065979:CDS |
| TCAGATTAAGGATATTATCA+TGG | 0.543580 | 4.3:+13284255 | MS.gene065979:CDS |
| TTATATTCCTGCAAGTACTC+CGG | 0.543933 | 4.3:-13285255 | None:intergenic |
| ATGTGTGGAGCTATTTGGAA+AGG | 0.549884 | 4.3:+13285070 | MS.gene065979:CDS |
| AACAAATGCATTGTACTGCT+CGG | 0.550416 | 4.3:+13284839 | MS.gene065979:CDS |
| TCTCCAACAACAACAATGGT+TGG | 0.551269 | 4.3:-13283884 | None:intergenic |
| GAGATTTGTAATGAACTTGG+TGG | 0.560761 | 4.3:+13285540 | MS.gene065979:CDS |
| TTCTTCACAATCAAAGTCAG+TGG | 0.564699 | 4.3:-13284148 | None:intergenic |
| TAGTTGATAAAACTGGTTTG+AGG | 0.569831 | 4.3:+13284368 | MS.gene065979:CDS |
| GTACTGCTCGGTTAGTGGAG+AGG | 0.571484 | 4.3:+13284851 | MS.gene065979:CDS |
| CTGTGGCCAACATTATGGAC+AGG | 0.573065 | 4.3:+13285658 | MS.gene065979:CDS |
| GACATTAGCTGTTGTAACAA+AGG | 0.576294 | 4.3:+13284390 | MS.gene065979:CDS |
| ATTACTTCAGAGAAAAGTGA+AGG | 0.577066 | 4.3:+13285014 | MS.gene065979:CDS |
| TTATCAGGTTGACCATGTAC+AGG | 0.577742 | 4.3:-13284223 | None:intergenic |
| TGCTTGCTGGAACTGCCAAA+GGG | 0.584310 | 4.3:+13284113 | MS.gene065979:CDS |
| CGTCTCAACATTGCTAAAGA+GGG | 0.585949 | 4.3:+13283851 | MS.gene065979:CDS |
| GTGGACAAGGAATTTGTACA+AGG | 0.592993 | 4.3:+13283963 | MS.gene065979:CDS |
| CAGTGTTGAGAGGCTGCTTG+AGG | 0.594487 | 4.3:+13285563 | MS.gene065979:CDS |
| GGAAGAGATCGTAGTCCTTG+AGG | 0.596070 | 4.3:+13284939 | MS.gene065979:CDS |
| ATTGGAAGATTGTGCTGAGG+AGG | 0.597029 | 4.3:+13285448 | MS.gene065979:CDS |
| ACTGTGAAGCTTGAAGGAGT+TGG | 0.602736 | 4.3:+13285342 | MS.gene065979:CDS |
| TGTGAGAAATCGCATTGGGG+AGG | 0.608396 | 4.3:+13284486 | MS.gene065979:CDS |
| GGTTGCTACTCGTAGAGCTG+TGG | 0.612719 | 4.3:+13285143 | MS.gene065979:CDS |
| TGTGTGGAGCTATTTGGAAA+GGG | 0.616400 | 4.3:+13285071 | MS.gene065979:CDS |
| CAATTAACTTAGCTTCCTCA+AGG | 0.618556 | 4.3:-13284954 | None:intergenic |
| ACAGGAACACGAGTTATACC+TGG | 0.620303 | 4.3:-13284205 | None:intergenic |
| CAAAACAGGAATACCAACAA+TGG | 0.623000 | 4.3:-13284584 | None:intergenic |
| GATTAACAATGATCTGCAGA+AGG | 0.625104 | 4.3:+13285518 | MS.gene065979:CDS |
| ACTGCTGATGATGTTAACAT+TGG | 0.625783 | 4.3:+13284448 | MS.gene065979:CDS |
| AAGGCTGATAAATCACCTGA+AGG | 0.627415 | 4.3:+13284409 | MS.gene065979:CDS |
| TTGATCTCCAACAACAACAA+TGG | 0.648908 | 4.3:-13283888 | None:intergenic |
| TAGAGAAAGCAAAGAGACTG+TGG | 0.654791 | 4.3:+13285641 | MS.gene065979:CDS |
| GTACAAATTCCTTGTCCACG+TGG | 0.660130 | 4.3:-13283959 | None:intergenic |
| TTACTTCAGAGAAAAGTGAA+GGG | 0.662697 | 4.3:+13285015 | MS.gene065979:CDS |
| TGTTGATATGGAGAAGCACA+CGG | 0.665028 | 4.3:+13285218 | MS.gene065979:CDS |
| AACTTGGTGGCAGTGTTGAG+AGG | 0.665158 | 4.3:+13285553 | MS.gene065979:CDS |
| CAGTAGCAGAAAGAACATTG+AGG | 0.676780 | 4.3:-13284306 | None:intergenic |
| GTTTGTGTGAGAAATCGCAT+TGG | 0.677044 | 4.3:+13284481 | MS.gene065979:CDS |
| TGGTGTTCCTGACCTTACAA+TGG | 0.692132 | 4.3:+13284174 | MS.gene065979:CDS |
| TGCTGAAAGTGATTCCACAA+AGG | 0.697479 | 4.3:+13284906 | MS.gene065979:CDS |
| GCTGGTATCAGCTTGCCACG+TGG | 0.699523 | 4.3:+13283944 | MS.gene065979:CDS |
| GTTGATCATCAACATCAACA+TGG | 0.706155 | 4.3:-13283745 | None:intergenic |
| TGGAAACAAGAGTTCCACAA+AGG | 0.707464 | 4.3:+13283685 | MS.gene065979:CDS |
| CCTATTGGAAGATTGTGCTG+AGG | 0.716472 | 4.3:+13285445 | MS.gene065979:CDS |
| TGGACAAGGAATTTGTACAA+GGG | 0.728664 | 4.3:+13283964 | MS.gene065979:CDS |
| TTGTGTGAGAAATCGCATTG+GGG | 0.740526 | 4.3:+13284483 | MS.gene065979:CDS |
| ACTGGTAATAGTTTGCAGAG+TGG | 0.741343 | 4.3:-13285116 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAACATTTATGATCAGATTA+AGG | + | chr4.3:13284243-13284262 | MS.gene065979:CDS | 20.0% |
| !! | TCATTATAAGAAGATACAAT+TGG | - | chr4.3:13283791-13283810 | None:intergenic | 20.0% |
| !!! | AGCATTTTTGAAAAAAGTTT+TGG | + | chr4.3:13285299-13285318 | MS.gene065979:CDS | 20.0% |
| ! | AATGCATTTGTTTATCATCA+GGG | - | chr4.3:13284831-13284850 | None:intergenic | 25.0% |
| ! | ACATTGTCAACAAAATTAGT+TGG | - | chr4.3:13285054-13285073 | None:intergenic | 25.0% |
| ! | ATCTGATCATAAATGTTATC+AGG | - | chr4.3:13284241-13284260 | None:intergenic | 25.0% |
| ! | TCAGATTAAGGATATTATCA+TGG | + | chr4.3:13284255-13284274 | MS.gene065979:CDS | 25.0% |
| ! | TTGATCTTCTTAATTATCTC+AGG | - | chr4.3:13284658-13284677 | None:intergenic | 25.0% |
| AAGATTGACAAATCCATTGT+TGG | + | chr4.3:13284571-13284590 | MS.gene065979:CDS | 30.0% | |
| AAGGAGATTTGTAATGAACT+TGG | + | chr4.3:13285537-13285556 | MS.gene065979:CDS | 30.0% | |
| AATGTTAGAAAAGCAGTTCT+TGG | - | chr4.3:13284997-13285016 | None:intergenic | 30.0% | |
| ACTTTGATTGTGAAGAAGAA+TGG | + | chr4.3:13284154-13284173 | MS.gene065979:CDS | 30.0% | |
| ATTACTTCAGAGAAAAGTGA+AGG | + | chr4.3:13285014-13285033 | MS.gene065979:CDS | 30.0% | |
| CAATGCATTTGTTTATCATC+AGG | - | chr4.3:13284832-13284851 | None:intergenic | 30.0% | |
| CTGAGAAAGATTCTTCTAAT+GGG | + | chr4.3:13284790-13284809 | MS.gene065979:CDS | 30.0% | |
| TCTGAGAAAGATTCTTCTAA+TGG | + | chr4.3:13284789-13284808 | MS.gene065979:CDS | 30.0% | |
| TTACTTCAGAGAAAAGTGAA+GGG | + | chr4.3:13285015-13285034 | MS.gene065979:CDS | 30.0% | |
| TTAGTACTTGTGAGTCTATT+AGG | + | chr4.3:13284335-13284354 | MS.gene065979:CDS | 30.0% | |
| TTCATTTCTTGCTTCTTCAT+AGG | - | chr4.3:13284515-13284534 | None:intergenic | 30.0% | |
| TTGATGCTCTTGAAAATCTA+AGG | + | chr4.3:13283828-13283847 | MS.gene065979:CDS | 30.0% | |
| ! | CTAATTTTGTTGACAATGTG+TGG | + | chr4.3:13285055-13285074 | MS.gene065979:CDS | 30.0% |
| ! | TGATGATGTTAACATTGGTT+TGG | + | chr4.3:13284453-13284472 | MS.gene065979:CDS | 30.0% |
| !! | GATGATGTTAACATTGGTTT+GGG | + | chr4.3:13284454-13284473 | MS.gene065979:CDS | 30.0% |
| !! | TAGTTGATAAAACTGGTTTG+AGG | + | chr4.3:13284368-13284387 | MS.gene065979:CDS | 30.0% |
| !! | TCACAGATAGTTGATAAAAC+TGG | + | chr4.3:13284361-13284380 | MS.gene065979:CDS | 30.0% |
| AACAAATGCATTGTACTGCT+CGG | + | chr4.3:13284839-13284858 | MS.gene065979:CDS | 35.0% | |
| AGGCAAGATTAATTGCCTAT+TGG | + | chr4.3:13285430-13285449 | MS.gene065979:CDS | 35.0% | |
| ATCAGCCAATGAAGTCAAAT+TGG | - | chr4.3:13284725-13284744 | None:intergenic | 35.0% | |
| ATTGGAGTATAATGGCAAAC+AGG | + | chr4.3:13284039-13284058 | MS.gene065979:CDS | 35.0% | |
| CAAAACAGGAATACCAACAA+TGG | - | chr4.3:13284587-13284606 | None:intergenic | 35.0% | |
| CAATTAACTTAGCTTCCTCA+AGG | - | chr4.3:13284957-13284976 | None:intergenic | 35.0% | |
| CTTGAGGAAGCTAAGTTAAT+TGG | + | chr4.3:13284955-13284974 | MS.gene065979:CDS | 35.0% | |
| GACATTAGCTGTTGTAACAA+AGG | + | chr4.3:13284390-13284409 | MS.gene065979:CDS | 35.0% | |
| GAGATTTGTAATGAACTTGG+TGG | + | chr4.3:13285540-13285559 | MS.gene065979:CDS | 35.0% | |
| GAGCTTGTATTGGAGTATAA+TGG | + | chr4.3:13284031-13284050 | MS.gene065979:CDS | 35.0% | |
| GATTAACAATGATCTGCAGA+AGG | + | chr4.3:13285518-13285537 | MS.gene065979:CDS | 35.0% | |
| GTAAGAGACAAAGTTTAAGC+AGG | + | chr4.3:13285604-13285623 | MS.gene065979:CDS | 35.0% | |
| GTTGATCATCAACATCAACA+TGG | - | chr4.3:13283748-13283767 | None:intergenic | 35.0% | |
| TAAATCACCTGAAGGTTTGT+TGG | + | chr4.3:13284417-13284436 | MS.gene065979:CDS | 35.0% | |
| TAATCTTGCCTTCAAGTCAA+AGG | - | chr4.3:13285421-13285440 | None:intergenic | 35.0% | |
| TGATACCAGCTAATGATTCA+AGG | - | chr4.3:13283934-13283953 | None:intergenic | 35.0% | |
| TGGACAAGGAATTTGTACAA+GGG | + | chr4.3:13283964-13283983 | MS.gene065979:CDS | 35.0% | |
| TGTCTCTTACCAGAAATAGA+TGG | - | chr4.3:13285594-13285613 | None:intergenic | 35.0% | |
| TTATACTCCAATACAAGCTC+TGG | - | chr4.3:13284031-13284050 | None:intergenic | 35.0% | |
| TTATATTCCTGCAAGTACTC+CGG | - | chr4.3:13285258-13285277 | None:intergenic | 35.0% | |
| TTCCACAAAGGATTTCCTAA+TGG | + | chr4.3:13284918-13284937 | MS.gene065979:CDS | 35.0% | |
| TTCCATTAGGAAATCCTTTG+TGG | - | chr4.3:13284923-13284942 | None:intergenic | 35.0% | |
| TTCTTCACAATCAAAGTCAG+TGG | - | chr4.3:13284151-13284170 | None:intergenic | 35.0% | |
| TTGATCTCCAACAACAACAA+TGG | - | chr4.3:13283891-13283910 | None:intergenic | 35.0% | |
| TTTGGAAAGGGTTGTTATCT+CGG | + | chr4.3:13285083-13285102 | MS.gene065979:CDS | 35.0% | |
| TTTGTGTGAGAAATCGCATT+GGG | + | chr4.3:13284482-13284501 | MS.gene065979:CDS | 35.0% | |
| ! | ACTGCTGATGATGTTAACAT+TGG | + | chr4.3:13284448-13284467 | MS.gene065979:CDS | 35.0% |
| ! | ATCAAGTTTGTTTGCCTTTG+TGG | - | chr4.3:13283702-13283721 | None:intergenic | 35.0% |
| ! | CATTGTTGGTATTCCTGTTT+TGG | + | chr4.3:13284585-13284604 | MS.gene065979:CDS | 35.0% |
| !! | AATTTGTACAAGGGTACCTT+TGG | + | chr4.3:13283973-13283992 | MS.gene065979:CDS | 35.0% |
| AAGGCTGATAAATCACCTGA+AGG | + | chr4.3:13284409-13284428 | MS.gene065979:CDS | 40.0% | |
| ACAATTGGTGCAACAGATAG+AGG | - | chr4.3:13283776-13283795 | None:intergenic | 40.0% | |
| ACTTTCTCCAACAAACCTTC+AGG | - | chr4.3:13284427-13284446 | None:intergenic | 40.0% | |
| AGATCAACCATTGTAAGGTC+AGG | - | chr4.3:13284184-13284203 | None:intergenic | 40.0% | |
| AGCTTGCATCACATGTTGAA+TGG | - | chr4.3:13285199-13285218 | None:intergenic | 40.0% | |
| AGTGTCCTTGAATCATTAGC+TGG | + | chr4.3:13283926-13283945 | MS.gene065979:CDS | 40.0% | |
| ATAACTCGTGTTCCTGTACA+TGG | + | chr4.3:13284211-13284230 | MS.gene065979:CDS | 40.0% | |
| ATGTGTGGAGCTATTTGGAA+AGG | + | chr4.3:13285070-13285089 | MS.gene065979:CDS | 40.0% | |
| CAGTAGCAGAAAGAACATTG+AGG | - | chr4.3:13284309-13284328 | None:intergenic | 40.0% | |
| CGTCTCAACATTGCTAAAGA+GGG | + | chr4.3:13283851-13283870 | MS.gene065979:CDS | 40.0% | |
| CTGGAAGATCAACCATTGTA+AGG | - | chr4.3:13284189-13284208 | None:intergenic | 40.0% | |
| CTTACAATGGTTGATCTTCC+AGG | + | chr4.3:13284187-13284206 | MS.gene065979:CDS | 40.0% | |
| GCAGTTCTTGGCATGAAATT+TGG | - | chr4.3:13284985-13285004 | None:intergenic | 40.0% | |
| GCCTGATTCAACACATTGAT+CGG | - | chr4.3:13285399-13285418 | None:intergenic | 40.0% | |
| GTGGACAAGGAATTTGTACA+AGG | + | chr4.3:13283963-13283982 | MS.gene065979:CDS | 40.0% | |
| GTTGTTGGAGATCAATCATC+TGG | + | chr4.3:13283896-13283915 | MS.gene065979:CDS | 40.0% | |
| GTTTGTGTGAGAAATCGCAT+TGG | + | chr4.3:13284481-13284500 | MS.gene065979:CDS | 40.0% | |
| TAGAGAAAGCAAAGAGACTG+TGG | + | chr4.3:13285641-13285660 | MS.gene065979:CDS | 40.0% | |
| TCCAATCCTGTCCATAATGT+TGG | - | chr4.3:13285667-13285686 | None:intergenic | 40.0% | |
| TCCGATCAATGTGTTGAATC+AGG | + | chr4.3:13285395-13285414 | MS.gene065979:CDS | 40.0% | |
| TCTCCAACAACAACAATGGT+TGG | - | chr4.3:13283887-13283906 | None:intergenic | 40.0% | |
| TGACAATGTGTGGAGCTATT+TGG | + | chr4.3:13285065-13285084 | MS.gene065979:CDS | 40.0% | |
| TGCAAACTATTACCAGTTGC+AGG | + | chr4.3:13285122-13285141 | MS.gene065979:CDS | 40.0% | |
| TGCTGAAAGTGATTCCACAA+AGG | + | chr4.3:13284906-13284925 | MS.gene065979:CDS | 40.0% | |
| TGGAAACAAGAGTTCCACAA+AGG | + | chr4.3:13283685-13283704 | MS.gene065979:CDS | 40.0% | |
| TGTGATGCAAGCTGTTGATA+TGG | + | chr4.3:13285206-13285225 | MS.gene065979:CDS | 40.0% | |
| TGTGTGGAGCTATTTGGAAA+GGG | + | chr4.3:13285071-13285090 | MS.gene065979:CDS | 40.0% | |
| TTGAGGATAATGCTCTCTTC+AGG | - | chr4.3:13284292-13284311 | None:intergenic | 40.0% | |
| TTGTGTGAGAAATCGCATTG+GGG | + | chr4.3:13284483-13284502 | MS.gene065979:CDS | 40.0% | |
| ! | ACTGGTAATAGTTTGCAGAG+TGG | - | chr4.3:13285119-13285138 | None:intergenic | 40.0% |
| ! | CTTCCAACCATTGTTGTTGT+TGG | + | chr4.3:13283881-13283900 | MS.gene065979:CDS | 40.0% |
| ! | TCAAGTACTGTGAAGCTTGA+AGG | + | chr4.3:13285336-13285355 | MS.gene065979:CDS | 40.0% |
| ! | TGGAGTGTTTGAAATCCCTT+TGG | - | chr4.3:13284131-13284150 | None:intergenic | 40.0% |
| ! | TGTTGATATGGAGAAGCACA+CGG | + | chr4.3:13285218-13285237 | MS.gene065979:CDS | 40.0% |
| ! | TTTTGCAGCCTCATAACCAA+AGG | - | chr4.3:13283992-13284011 | None:intergenic | 40.0% |
| !! | TTATCAGGTTGACCATGTAC+AGG | - | chr4.3:13284226-13284245 | None:intergenic | 40.0% |
| !!! | TTTTCAAGAGCATCAAGCAC+TGG | - | chr4.3:13283824-13283843 | None:intergenic | 40.0% |
| ACAAGCTTCTGAGCCAAAAC+AGG | - | chr4.3:13284601-13284620 | None:intergenic | 45.0% | |
| ACAGGAACACGAGTTATACC+TGG | - | chr4.3:13284208-13284227 | None:intergenic | 45.0% | |
| ACTGTGAAGCTTGAAGGAGT+TGG | + | chr4.3:13285342-13285361 | MS.gene065979:CDS | 45.0% | |
| AGAGACTGTGGCCAACATTA+TGG | + | chr4.3:13285653-13285672 | MS.gene065979:CDS | 45.0% | |
| ATGGACAGGATTGGAGTCTA+TGG | + | chr4.3:13285672-13285691 | MS.gene065979:CDS | 45.0% | |
| ATTGGAAGATTGTGCTGAGG+AGG | + | chr4.3:13285448-13285467 | MS.gene065979:CDS | 45.0% | |
| CCTATTGGAAGATTGTGCTG+AGG | + | chr4.3:13285445-13285464 | MS.gene065979:CDS | 45.0% | |
| CCTCAGCACAATCTTCCAAT+AGG | - | chr4.3:13285448-13285467 | None:intergenic | 45.0% | |
| GAGGAGTCTCCATCTATTTC+TGG | + | chr4.3:13285582-13285601 | MS.gene065979:CDS | 45.0% | |
| GCCAACATTATGGACAGGAT+TGG | + | chr4.3:13285663-13285682 | MS.gene065979:CDS | 45.0% | |
| GCCAATGAAGTCAAATTGGC+AGG | - | chr4.3:13284721-13284740 | None:intergenic | 45.0% | |
| GCGTCTCAACATTGCTAAAG+AGG | + | chr4.3:13283850-13283869 | MS.gene065979:CDS | 45.0% | |
| GGACTACGATCTCTTCCATT+AGG | - | chr4.3:13284936-13284955 | None:intergenic | 45.0% | |
| GTACAAATTCCTTGTCCACG+TGG | - | chr4.3:13283962-13283981 | None:intergenic | 45.0% | |
| TGGTGTTCCTGACCTTACAA+TGG | + | chr4.3:13284174-13284193 | MS.gene065979:CDS | 45.0% | |
| ! | CACGGATTACACTTGCAATC+CGG | + | chr4.3:13285236-13285255 | MS.gene065979:CDS | 45.0% |
| ! | GCCTGCCAATTTGACTTCAT+TGG | + | chr4.3:13284717-13284736 | MS.gene065979:CDS | 45.0% |
| !! | CAAGGGTACCTTTGGTTATG+AGG | + | chr4.3:13283981-13284000 | MS.gene065979:CDS | 45.0% |
| !! | GAATCAGGCCTTTGACTTGA+AGG | + | chr4.3:13285410-13285429 | MS.gene065979:CDS | 45.0% |
| CTGTGGCCAACATTATGGAC+AGG | + | chr4.3:13285658-13285677 | MS.gene065979:CDS | 50.0% | |
| GCATTGTACTGCTCGGTTAG+TGG | + | chr4.3:13284846-13284865 | MS.gene065979:CDS | 50.0% | |
| GCTCTGGCTGTGGAAGTAAA+TGG | - | chr4.3:13284015-13284034 | None:intergenic | 50.0% | |
| GGAAGAGATCGTAGTCCTTG+AGG | + | chr4.3:13284939-13284958 | MS.gene065979:CDS | 50.0% | |
| GGTGCAACAGATAGAGGTTG+AGG | - | chr4.3:13283770-13283789 | None:intergenic | 50.0% | |
| TACGAGTAGCAACCTGCAAC+TGG | - | chr4.3:13285137-13285156 | None:intergenic | 50.0% | |
| TCCACAGCCAGAGCTTGTAT+TGG | + | chr4.3:13284021-13284040 | MS.gene065979:CDS | 50.0% | |
| TGTGAGAAATCGCATTGGGG+AGG | + | chr4.3:13284486-13284505 | MS.gene065979:CDS | 50.0% | |
| ! | AACTTGGTGGCAGTGTTGAG+AGG | + | chr4.3:13285553-13285572 | MS.gene065979:CDS | 50.0% |
| ! | ACAGCTACTGAAGTGCTTGC+TGG | + | chr4.3:13284100-13284119 | MS.gene065979:CDS | 50.0% |
| ! | TCCAATACAAGCTCTGGCTG+TGG | - | chr4.3:13284025-13284044 | None:intergenic | 50.0% |
| ! | TGCTTGCTGGAACTGCCAAA+GGG | + | chr4.3:13284113-13284132 | MS.gene065979:CDS | 50.0% |
| ! | TTGCAATCCGGAGTACTTGC+AGG | + | chr4.3:13285248-13285267 | MS.gene065979:CDS | 50.0% |
| ATCAGCTTGCCACGTGGACA+AGG | + | chr4.3:13283950-13283969 | MS.gene065979:CDS | 55.0% | |
| GGTTGCTACTCGTAGAGCTG+TGG | + | chr4.3:13285143-13285162 | MS.gene065979:CDS | 55.0% | |
| GTACTGCTCGGTTAGTGGAG+AGG | + | chr4.3:13284851-13284870 | MS.gene065979:CDS | 55.0% | |
| ! | CAGTGTTGAGAGGCTGCTTG+AGG | + | chr4.3:13285563-13285582 | MS.gene065979:CDS | 55.0% |
| ! | GTGCTTGCTGGAACTGCCAA+AGG | + | chr4.3:13284112-13284131 | MS.gene065979:CDS | 55.0% |
| GCTGGTATCAGCTTGCCACG+TGG | + | chr4.3:13283944-13283963 | MS.gene065979:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 13283677 | 13285704 | 13283677 | ID=MS.gene065979 |
| chr4.3 | mRNA | 13283677 | 13285704 | 13283677 | ID=MS.gene065979.t1;Parent=MS.gene065979 |
| chr4.3 | exon | 13283677 | 13285704 | 13283677 | ID=MS.gene065979.t1.exon1;Parent=MS.gene065979.t1 |
| chr4.3 | CDS | 13283677 | 13285704 | 13283677 | ID=cds.MS.gene065979.t1;Parent=MS.gene065979.t1 |
| Gene Sequence |
| Protein sequence |
