AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene26380


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene26380.t1 MTR_4g088385 96.104 154 2 3 1 152 207 358 5.53e-91 268
MS.gene26380.t1 MTR_2g014400 52.381 147 56 5 13 151 120 260 1.76e-18 79.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 16 sgRNAs with CRISPR-Local

Find 20 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCAAACTGCTCTTGATTC+TGG 0.250818 4.1:+21896937 None:intergenic
TGTTGTTGTTGTTATTGTTC+TGG 0.280406 4.1:+21897339 None:intergenic
ATTGAGTTGTAACCTTCTTC+TGG 0.340437 4.1:+21897272 None:intergenic
TTGAGTTGTAACCTTCTTCT+GGG 0.440468 4.1:+21897273 None:intergenic
TTCAATGGAGATTCATCTAA+AGG 0.457617 4.1:-21897056 MS.gene26380:CDS
AATGAGTGACACAAGAGTTA+TGG 0.465302 4.1:-21897132 MS.gene26380:CDS
AAATACAATCCAACATTCAA+TGG 0.477159 4.1:-21897071 MS.gene26380:CDS
GAGTTGTGAACTCTGAGAAT+TGG 0.491359 4.1:-21897034 MS.gene26380:CDS
GATGAATCTCCATTGAATGT+TGG 0.492045 4.1:+21897062 None:intergenic
AACACAAAGTTCCCAGAAGA+AGG 0.504928 4.1:-21897284 MS.gene26380:CDS
AATAACAAGAACTCTATGAA+TGG 0.533344 4.1:-21896963 MS.gene26380:CDS
TACAACAATGGTGAGAGGCA+AGG 0.586180 4.1:-21897155 MS.gene26380:CDS
AGTAACAACAATTACAACAA+TGG 0.621284 4.1:-21897167 MS.gene26380:CDS
GACACAAGAGTTATGGAAGG+TGG 0.628338 4.1:-21897125 MS.gene26380:CDS
ACAATTACAACAATGGTGAG+AGG 0.652438 4.1:-21897160 MS.gene26380:CDS
AGTGACACAAGAGTTATGGA+AGG 0.692016 4.1:-21897128 MS.gene26380:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AAATACAATCCAACATTCAA+TGG - chr4.1:21897187-21897206 MS.gene26380:CDS 25.0%
! AATAACAAGAACTCTATGAA+TGG - chr4.1:21897295-21897314 MS.gene26380:CDS 25.0%
! AGTAACAACAATTACAACAA+TGG - chr4.1:21897091-21897110 MS.gene26380:CDS 25.0%
! TACAACAAAAAAGGTTACTT+TGG - chr4.1:21897247-21897266 MS.gene26380:CDS 25.0%
!! TTTTTATGATGTTAACTCTG+AGG - chr4.1:21897162-21897181 MS.gene26380:CDS 25.0%
GAGAATTGGTACAACAAAAA+AGG - chr4.1:21897238-21897257 MS.gene26380:CDS 30.0%
TTCAATGGAGATTCATCTAA+AGG - chr4.1:21897202-21897221 MS.gene26380:CDS 30.0%
! TGTTGTTGTTGTTATTGTTC+TGG + chr4.1:21896922-21896941 None:intergenic 30.0%
AATGAGTGACACAAGAGTTA+TGG - chr4.1:21897126-21897145 MS.gene26380:CDS 35.0%
ACAATTACAACAATGGTGAG+AGG - chr4.1:21897098-21897117 MS.gene26380:CDS 35.0%
GATGAATCTCCATTGAATGT+TGG + chr4.1:21897199-21897218 None:intergenic 35.0%
! ATTGAGTTGTAACCTTCTTC+TGG + chr4.1:21896989-21897008 None:intergenic 35.0%
! TTGAGTTGTAACCTTCTTCT+GGG + chr4.1:21896988-21897007 None:intergenic 35.0%
AACACAAAGTTCCCAGAAGA+AGG - chr4.1:21896974-21896993 MS.gene26380:CDS 40.0%
AGTGACACAAGAGTTATGGA+AGG - chr4.1:21897130-21897149 MS.gene26380:CDS 40.0%
CTTCAAACTGCTCTTGATTC+TGG + chr4.1:21897324-21897343 None:intergenic 40.0%
! CGTAGTTGTTGTTGTTGTTC+TGG + chr4.1:21897018-21897037 None:intergenic 40.0%
! GAGTTGTGAACTCTGAGAAT+TGG - chr4.1:21897224-21897243 MS.gene26380:CDS 40.0%
GACACAAGAGTTATGGAAGG+TGG - chr4.1:21897133-21897152 MS.gene26380:CDS 45.0%
TACAACAATGGTGAGAGGCA+AGG - chr4.1:21897103-21897122 MS.gene26380:CDS 45.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 21896911 21897369 21896911 ID=MS.gene26380
chr4.1 mRNA 21896911 21897369 21896911 ID=MS.gene26380.t1;Parent=MS.gene26380
chr4.1 exon 21896911 21897369 21896911 ID=MS.gene26380.t1.exon1;Parent=MS.gene26380.t1
chr4.1 CDS 21896911 21897369 21896911 ID=cds.MS.gene26380.t1;Parent=MS.gene26380.t1
Gene Sequence

>MS.gene26380

ATGGAAAACCAGAACAATAACAACAACAACAAGTATTTCTACAACAACAAAGATGCTTCTAGCAACACAAAGTTCCCAGAAGAAGGTTACAACTCAATGCAAAACCAGAACAACAACAACAACTACGAGAAGTATAACTACAACAACAAAGTTGCTGTTAATGACAAATACAGCTTCAAAAGTAACAACAATTACAACAATGGTGAGAGGCAAGGAATGAGTGACACAAGAGTTATGGAAGGTGGAAAATATTTTTATGATGTTAACTCTGAGGAGAAATACAATCCAACATTCAATGGAGATTCATCTAAAGGAGTTGTGAACTCTGAGAATTGGTACAACAAAAAAGGTTACTTTGGTAACAACAATGTGAACAATTATGAGAATAACAAGAACTCTATGAATGGTTACCAGAATCAAGAGCAGTTTGAAGATGATCAAGAAGAATTTGAGCCTTGA

Protein sequence

>MS.gene26380.t1

MENQNNNNNNKYFYNNKDASSNTKFPEEGYNSMQNQNNNNNYEKYNYNNKVAVNDKYSFKSNNNYNNGERQGMSDTRVMEGGKYFYDVNSEEKYNPTFNGDSSKGVVNSENWYNKKGYFGNNNVNNYENNKNSMNGYQNQEQFEDDQEEFEP