AlfalfaGEDB | MS.gene77149

M. sativa cultivar XinJiangDaYe / MS.gene77149

KEGG: K07937
GO: GO:0000166 GO:0001882 GO:0001883 GO:0003674 GO:0005488 GO:0005525 GO:0017076 GO:0019001 GO:0032549 GO:0032550 GO:0032553 GO:0032555 GO:0032561 GO:0035639 GO:0036094 GO:0043167 GO:0043168 GO:0097159 GO:0097367 GO:1901265 GO:1901363
PFAM: PF00025.24

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene77149.t1	XP_003591092.2	92.9	84	6	0	142	225	186	269	1.70E-36	162.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene77149.t1	P49076	69.6	112	10	2	22	109	2	113	1.7e-29	131.0

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene77149.t1	G7I822	92.9	84	6	0	142	225	186	269	1.2e-36	162.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049142	MS.gene77149	0.827931	1.19E-54	-1.69E-46
MS.gene049159	MS.gene77149	0.850735	1.40E-60	-1.69E-46
MS.gene049254	MS.gene77149	0.824564	7.54E-54	-1.69E-46
MS.gene049501	MS.gene77149	0.814181	1.76E-51	-1.69E-46
MS.gene049850	MS.gene77149	0.806137	9.52E-50	-1.69E-46
MS.gene049983	MS.gene77149	0.803461	3.45E-49	-1.69E-46
MS.gene050221	MS.gene77149	0.803183	3.94E-49	-1.69E-46
MS.gene050442	MS.gene77149	0.81313	2.99E-51	-1.69E-46
MS.gene050826	MS.gene77149	0.807089	5.99E-50	-1.69E-46
MS.gene051147	MS.gene77149	0.806329	8.67E-50	-1.69E-46
MS.gene051198	MS.gene77149	0.813654	2.29E-51	-1.69E-46
MS.gene051247	MS.gene77149	0.805074	1.59E-49	-1.69E-46
MS.gene051388	MS.gene77149	0.801253	9.82E-49	-1.69E-46
MS.gene051800	MS.gene77149	0.838785	2.34E-57	-1.69E-46
MS.gene052042	MS.gene77149	0.800663	1.30E-48	-1.69E-46
MS.gene052234	MS.gene77149	0.801443	8.98E-49	-1.69E-46
MS.gene052463	MS.gene77149	0.804288	2.32E-49	-1.69E-46
MS.gene052516	MS.gene77149	0.811362	7.27E-51	-1.69E-46
MS.gene052773	MS.gene77149	0.802631	5.12E-49	-1.69E-46
MS.gene052863	MS.gene77149	0.821851	3.24E-53	-1.69E-46
MS.gene052896	MS.gene77149	0.809908	1.50E-50	-1.69E-46
MS.gene053621	MS.gene77149	0.812839	3.46E-51	-1.69E-46
MS.gene054236	MS.gene77149	0.854594	1.11E-61	-1.69E-46
MS.gene054467	MS.gene77149	0.815563	8.67E-52	-1.69E-46
MS.gene05458	MS.gene77149	0.807538	4.81E-50	-1.69E-46
MS.gene054651	MS.gene77149	0.82396	1.05E-53	-1.69E-46
MS.gene054775	MS.gene77149	0.80778	4.28E-50	-1.69E-46
MS.gene054801	MS.gene77149	0.801887	7.28E-49	-1.69E-46
MS.gene055227	MS.gene77149	0.802977	4.34E-49	-1.69E-46
MS.gene055228	MS.gene77149	0.811262	7.65E-51	-1.69E-46
MS.gene055466	MS.gene77149	0.812868	3.41E-51	-1.69E-46
MS.gene055864	MS.gene77149	0.805723	1.16E-49	-1.69E-46
MS.gene055875	MS.gene77149	0.81861	1.79E-52	-1.69E-46
MS.gene055926	MS.gene77149	0.83378	4.38E-56	-1.69E-46
MS.gene056174	MS.gene77149	0.820938	5.26E-53	-1.69E-46
MS.gene056595	MS.gene77149	0.805738	1.15E-49	-1.69E-46
MS.gene057915	MS.gene77149	0.820104	8.18E-53	-1.69E-46
MS.gene058010	MS.gene77149	0.838527	2.73E-57	-1.69E-46
MS.gene058339	MS.gene77149	0.802448	5.58E-49	-1.69E-46
MS.gene058979	MS.gene77149	0.808707	2.71E-50	-1.69E-46
MS.gene059272	MS.gene77149	0.806816	6.85E-50	-1.69E-46
MS.gene059283	MS.gene77149	0.813686	2.26E-51	-1.69E-46
MS.gene059570	MS.gene77149	0.814282	1.67E-51	-1.69E-46
MS.gene059842	MS.gene77149	0.804147	2.48E-49	-1.69E-46
MS.gene060011	MS.gene77149	0.835713	1.43E-56	-1.69E-46
MS.gene060167	MS.gene77149	0.838853	2.25E-57	-1.69E-46
MS.gene060312	MS.gene77149	0.80733	5.33E-50	-1.69E-46
MS.gene060322	MS.gene77149	0.808323	3.28E-50	-1.69E-46
MS.gene06052	MS.gene77149	0.841657	4.16E-58	-1.69E-46
MS.gene060803	MS.gene77149	0.800853	1.19E-48	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene77149.t1	MTR_1g082740	92.857	84	6	0	142	225	186	269	1.42e-51	168
MS.gene77149.t1	MTR_6g005820	69.643	112	10	2	22	109	2	113	4.23e-41	138
MS.gene77149.t1	MTR_5g034130	69.643	112	10	2	22	109	2	113	4.56e-41	138
MS.gene77149.t1	MTR_5g034125	69.643	112	10	2	22	109	2	113	4.56e-41	138
MS.gene77149.t1	MTR_7g109960	68.750	112	11	2	22	109	2	113	1.44e-40	137
MS.gene77149.t1	MTR_1g086480	68.750	112	11	2	22	109	2	113	1.58e-40	137
MS.gene77149.t1	MTR_5g016540	58.036	112	23	2	22	109	2	113	1.68e-32	116
MS.gene77149.t1	MTR_4g128910	45.536	112	37	2	22	109	2	113	1.13e-22	91.3
MS.gene77149.t1	MTR_5g007810	35.075	134	63	1	22	131	2	135	4.10e-20	84.7
MS.gene77149.t1	MTR_4g077640	35.075	134	63	2	22	131	2	135	2.24e-19	82.8
MS.gene77149.t1	MTR_5g007820	33.824	136	64	1	22	131	2	137	3.89e-19	82.0
MS.gene77149.t1	MTR_5g007840	35.152	165	73	5	22	171	2	147	6.73e-19	81.6
MS.gene77149.t1	MTR_1g076550	37.500	112	46	2	22	109	2	113	5.04e-14	68.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene77149.t1	AT5G14670	68.750	112	11	2	22	109	2	113	2.20e-40	137
MS.gene77149.t1	AT5G14670	68.750	112	11	2	22	109	2	113	2.20e-40	137
MS.gene77149.t1	AT5G14670	68.750	112	11	2	22	109	2	113	3.84e-40	136
MS.gene77149.t1	AT1G10630	67.857	112	12	2	22	109	2	113	7.86e-40	135
MS.gene77149.t1	AT1G10630	67.857	112	12	2	22	109	2	113	7.86e-40	135
MS.gene77149.t1	AT1G70490	67.857	112	12	2	22	109	2	113	8.12e-40	135
MS.gene77149.t1	AT1G70490	67.857	112	12	2	22	109	2	113	8.12e-40	135
MS.gene77149.t1	AT1G70490	67.857	112	12	2	22	109	2	113	8.12e-40	135
MS.gene77149.t1	AT1G70490	67.857	112	12	2	22	109	2	113	8.12e-40	135
MS.gene77149.t1	AT1G70490	67.857	112	12	2	22	109	2	113	8.12e-40	135
MS.gene77149.t1	AT1G70490	67.857	112	12	2	22	109	2	113	8.12e-40	135
MS.gene77149.t1	AT1G23490	67.857	112	12	2	22	109	2	113	8.12e-40	135
MS.gene77149.t1	AT1G70490	67.857	112	12	2	22	109	2	113	8.12e-40	135
MS.gene77149.t1	AT2G47170	67.857	112	12	2	22	109	2	113	1.29e-39	135
MS.gene77149.t1	AT2G47170	67.857	112	12	2	22	109	2	113	1.29e-39	135
MS.gene77149.t1	AT2G47170	67.857	112	12	2	22	109	2	113	1.29e-39	135
MS.gene77149.t1	AT3G62290	66.964	112	13	2	22	109	2	113	4.29e-39	133
MS.gene77149.t1	AT3G62290	66.964	112	13	2	22	109	2	113	4.29e-39	133
MS.gene77149.t1	AT3G62290	66.964	112	13	2	22	109	2	113	4.29e-39	133
MS.gene77149.t1	AT2G15310	46.429	112	36	2	22	109	2	113	7.33e-23	92.4
MS.gene77149.t1	AT2G24765	41.964	112	41	2	22	109	2	113	5.86e-21	85.5
MS.gene77149.t1	AT2G24765	41.964	112	41	2	22	109	2	113	1.34e-20	85.9
MS.gene77149.t1	AT3G03120	40.496	121	42	2	22	112	2	122	2.46e-20	84.0
MS.gene77149.t1	AT3G03120	35.821	134	62	1	22	131	2	135	4.83e-20	84.7
MS.gene77149.t1	AT5G17060	40.496	121	42	2	22	112	2	122	1.59e-19	83.2
MS.gene77149.t1	AT5G17060	40.496	121	42	2	22	112	2	122	1.59e-19	83.2
MS.gene77149.t1	AT3G05250	48.837	86	39	1	142	222	124	209	1.53e-17	78.2
MS.gene77149.t1	AT3G05250	48.837	86	39	1	142	222	124	209	1.53e-17	78.2
MS.gene77149.t1	AT3G05250	48.837	86	39	1	142	222	193	278	4.29e-17	78.2
MS.gene77149.t1	AT1G02430	37.069	116	46	3	22	110	2	117	3.79e-15	70.9
MS.gene77149.t1	AT1G02430	37.069	116	46	3	22	110	2	117	5.37e-15	70.9
MS.gene77149.t1	AT3G22950	36.607	112	47	2	22	109	2	113	1.46e-13	67.0
MS.gene77149.t1	AT3G22950	36.607	112	47	2	22	109	2	113	1.46e-13	67.0

Find 54 sgRNAs with CRISPR-Local

Find 155 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATTCAACTATTCCTTGCTTC+AGG	0.313766	2.4:+32116469	MS.gene77149:CDS
TAAATCAGCTAGGCCTTTCC+TGG	0.324763	2.4:+32116417	MS.gene77149:CDS
GCGGTTTATATTCAACGATT+AGG	0.330129	2.4:+32116514	MS.gene77149:CDS
GTCACCACCATTCCTACTAT+TGG	0.339573	2.4:+32114930	MS.gene77149:CDS
CAGTCGGTTATTTGCGAAGA+AGG	0.396684	2.4:+32114830	MS.gene77149:CDS
TCCACAAGGGAAAGACAAAA+AGG	0.398388	2.4:-32115852	None:intergenic
CGATTAGGATGGAGCTCACC+AGG	0.401263	2.4:+32116529	MS.gene77149:CDS
TGATGTTTCTCAAAATGAAT+TGG	0.413515	2.4:+32116561	MS.gene77149:CDS
ACTATTCCTTGCTTCAGGAT+TGG	0.433302	2.4:+32116474	MS.gene77149:CDS
AATCTCATACACAAATTTGT+CGG	0.438859	2.4:-32116449	None:intergenic
AATTTCTGTCGAGCTTCTCA+TGG	0.449121	2.4:+32116179	MS.gene77149:CDS
TAGCCAACAGTGTGTAGATA+TGG	0.449762	2.4:-32113639	None:intergenic
CAAGGAAGTGAAGGAGAAAA+CGG	0.456890	2.4:+32114785	MS.gene77149:CDS
ATTAACAGCAACAATGATGA+AGG	0.468264	2.4:-32116212	None:intergenic
TTGAAATTTAGGTTTGATCA+AGG	0.476869	2.4:+32114767	MS.gene77149:intron
TCGGTTCTTGCTCCCAGGAA+AGG	0.483050	2.4:-32116430	None:intergenic
AAGGAAGTGAAGGAGAAAAC+GGG	0.483308	2.4:+32114786	MS.gene77149:CDS
CATCCTATGTAGCTCATCTC+TGG	0.489228	2.4:-32115924	None:intergenic
TCATCAAATATGTACAGGTA+TGG	0.500375	2.4:-32116604	None:intergenic
ATTCTCTACAAGCTCAAGCT+TGG	0.503263	2.4:+32114900	MS.gene77149:CDS
AGGTTTGATCAAGGAAGTGA+AGG	0.505738	2.4:+32114776	MS.gene77149:intron
TTTATATTCAACGATTAGGA+TGG	0.507524	2.4:+32116518	MS.gene77149:CDS
TTCTAGATCATAATGTTCAC+TGG	0.508166	2.4:+32116127	MS.gene77149:intron
ATTTGTCGGTTCTTGCTCCC+AGG	0.509474	2.4:-32116435	None:intergenic
CCGAGAAACTTACCAATAGT+AGG	0.510028	2.4:-32114942	None:intergenic
AGTCATCATCAAATATGTAC+AGG	0.530474	2.4:-32116609	None:intergenic
AAGCTTGAGCTTGTAGAGAA+TGG	0.532263	2.4:-32114898	None:intergenic
TTGTCGTTGACAGTAATGAC+AGG	0.532677	2.4:+32115885	MS.gene77149:CDS
AAACTTACCAATAGTAGGAA+TGG	0.549558	2.4:-32114937	None:intergenic
AGGCCAGAGATGAGCTACAT+AGG	0.552666	2.4:+32115921	MS.gene77149:CDS
GAAGGAAATGCGAATTCTGA+TGG	0.554686	2.4:+32114848	MS.gene77149:CDS
CAACCATATCTACACACTGT+TGG	0.557182	2.4:+32113636	MS.gene77149:CDS
CAGTGTGTAGATATGGTTGA+AGG	0.559011	2.4:-32113632	None:intergenic
ATGGTTGGTCTCGATGCAGC+TGG	0.564897	2.4:+32114867	MS.gene77149:CDS
CATTCACGAAGCTGTTCAGT+CGG	0.566942	2.4:+32114814	MS.gene77149:CDS
TGGTTCAATGCGTGTCTCTG+TGG	0.582648	2.4:+32116147	MS.gene77149:CDS
GAAATGCGAATTCTGATGGT+TGG	0.586975	2.4:+32114852	MS.gene77149:CDS
ATTTCTGTCGAGCTTCTCAT+GGG	0.587081	2.4:+32116180	MS.gene77149:CDS
TGACAGGGATAGAGTTGTTG+AGG	0.602509	2.4:+32115901	MS.gene77149:CDS
CAATATCCAATCCTGAAGCA+AGG	0.603049	2.4:-32116480	None:intergenic
ATATTTGATGATGACTCTGA+TGG	0.611424	2.4:+32116616	MS.gene77149:CDS
AAATCAGCTAGGCCTTTCCT+GGG	0.618873	2.4:+32116418	MS.gene77149:CDS
CTTACCAATAGTAGGAATGG+TGG	0.621045	2.4:-32114934	None:intergenic
TGTCAGTCTCTAAATCAGCT+AGG	0.625702	2.4:+32116407	MS.gene77149:CDS
GGATATTGAAGCTTATGATG+CGG	0.631103	2.4:+32116495	MS.gene77149:CDS
GCTACATAGGATGTTGAATG+AGG	0.633182	2.4:+32115934	MS.gene77149:CDS
GATTAGGATGGAGCTCACCA+GGG	0.636076	2.4:+32116530	MS.gene77149:CDS
TTAACAGCAACAATGATGAA+GGG	0.636922	2.4:-32116211	None:intergenic
CTTGAGCTTGTAGAGAATGG+TGG	0.640954	2.4:-32114895	None:intergenic
TGTCGTTGACAGTAATGACA+GGG	0.667156	2.4:+32115886	MS.gene77149:CDS
ACAAATATAAGACCTCCACA+AGG	0.671284	2.4:-32115866	None:intergenic
ATTAGGATGGAGCTCACCAG+GGG	0.691130	2.4:+32116531	MS.gene77149:CDS
CAAATATAAGACCTCCACAA+GGG	0.698326	2.4:-32115865	None:intergenic
AGGAAGTGAAGGAGAAAACG+GGG	0.760741	2.4:+32114787	MS.gene77149:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTATTTTATATTTTAATTTA+GGG	+	chr2.4:32115693-32115712	MS.gene77149:intron	0.0%
!!!	TTTATTTTATATTTTAATTT+AGG	+	chr2.4:32115692-32115711	MS.gene77149:intron	0.0%
!!	GTACAAATAAAAAAAAAATA+TGG	-	chr2.4:32114065-32114084	None:intergenic	10.0%
!!	GATTTCATTGTTAAATATAA+AGG	-	chr2.4:32115335-32115354	None:intergenic	15.0%
!!!	ATGTTTATTTTAAACTTGAT+GGG	+	chr2.4:32115561-32115580	MS.gene77149:intron	15.0%
!!!	TATGAATAATGTTTATGTTT+TGG	+	chr2.4:32114722-32114741	MS.gene77149:intron	15.0%
!!!	TGAAATTTTGATTGAAATTT+AGG	+	chr2.4:32114756-32114775	MS.gene77149:intron	15.0%
!!	ACAATATATGACATAAAGTT+TGG	-	chr2.4:32115358-32115377	None:intergenic	20.0%
!!	ATCAATAGAAGATTTCAATT+AGG	-	chr2.4:32113710-32113729	None:intergenic	20.0%
!!	CAATATATGACATAAAGTTT+GGG	-	chr2.4:32115357-32115376	None:intergenic	20.0%
!!	TAATCACAACATATAAAGAT+TGG	-	chr2.4:32114027-32114046	None:intergenic	20.0%
!!!	AAAAAAATATGGACTAATCA+TGG	-	chr2.4:32114054-32114073	None:intergenic	20.0%
!!!	CATCAAGTTTAAAATAAACA+TGG	-	chr2.4:32115562-32115581	None:intergenic	20.0%
!!!	CATGTTTATTTTAAACTTGA+TGG	+	chr2.4:32115560-32115579	MS.gene77149:intron	20.0%
!!!	TACTTTTCTTATATTCAGTT+TGG	+	chr2.4:32115957-32115976	MS.gene77149:intron	20.0%
!	AATCTCATACACAAATTTGT+CGG	-	chr2.4:32116452-32116471	None:intergenic	25.0%
!	ACAACATATATGCTAACAAA+AGG	-	chr2.4:32113907-32113926	None:intergenic	25.0%
!	ACAACTATAATGACTTAGTT+TGG	+	chr2.4:32115419-32115438	MS.gene77149:intron	25.0%
!	CTTCAAAAGGAAATCAAAAT+TGG	-	chr2.4:32113783-32113802	None:intergenic	25.0%
!	GAATTGAGATAGAAAGATAT+AGG	+	chr2.4:32116036-32116055	MS.gene77149:intron	25.0%
!	TAAACATGGATGAAATTACA+TGG	-	chr2.4:32115548-32115567	None:intergenic	25.0%
!	TCTCAATTGAAATATGCATT+TGG	+	chr2.4:32115466-32115485	MS.gene77149:intron	25.0%
!	TGATGTTTCTCAAAATGAAT+TGG	+	chr2.4:32116561-32116580	MS.gene77149:CDS	25.0%
!	TTCTACAACTAATAAAGTTG+AGG	-	chr2.4:32113939-32113958	None:intergenic	25.0%
!	TTTATATTCAACGATTAGGA+TGG	+	chr2.4:32116518-32116537	MS.gene77149:CDS	25.0%
!!	ATAATGACTTAGTTTGGATT+AGG	+	chr2.4:32115425-32115444	MS.gene77149:intron	25.0%
!!	CTGTATATTAATCTGTCTTT+TGG	+	chr2.4:32116274-32116293	MS.gene77149:intron	25.0%
!!	TTGAAATTTAGGTTTGATCA+AGG	+	chr2.4:32114767-32114786	MS.gene77149:intron	25.0%
!!!	AATGTATGTATTGATTGCTT+TGG	+	chr2.4:32115206-32115225	MS.gene77149:intron	25.0%
!!!	ATGTATTAGCGTTTTGATTT+AGG	+	chr2.4:32114675-32114694	MS.gene77149:intron	25.0%
!!!	TAATTAATCCATGTTTTTGC+TGG	+	chr2.4:32116231-32116250	MS.gene77149:intron	25.0%
!!!	TTTTAATTTAGGGTTCAATG+TGG	+	chr2.4:32115703-32115722	MS.gene77149:intron	25.0%
	AAACTTACCAATAGTAGGAA+TGG	-	chr2.4:32114940-32114959	None:intergenic	30.0%
	AATATGATCCAGCAAAAACA+TGG	-	chr2.4:32116242-32116261	None:intergenic	30.0%
	AATCACAACCATCAGATAAT+TGG	+	chr2.4:32114153-32114172	MS.gene77149:intron	30.0%
	AATTCTCTAATCTCTGAATC+AGG	+	chr2.4:32116372-32116391	MS.gene77149:intron	30.0%
	ACAAAGTACATACAAATGAG+AGG	+	chr2.4:32114083-32114102	MS.gene77149:intron	30.0%
	AGTCATCATCAAATATGTAC+AGG	-	chr2.4:32116612-32116631	None:intergenic	30.0%
	ATTAACAGCAACAATGATGA+AGG	-	chr2.4:32116215-32116234	None:intergenic	30.0%
	ATTCTCAACAAATTTGTGGA+TGG	-	chr2.4:32116020-32116039	None:intergenic	30.0%
	CAAATCATAAGAATGTCTTC+AGG	-	chr2.4:32113832-32113851	None:intergenic	30.0%
	CTCAATTCTCAACAAATTTG+TGG	-	chr2.4:32116024-32116043	None:intergenic	30.0%
	GAATTAACATGCAAAGAAGT+CGG	-	chr2.4:32115247-32115266	None:intergenic	30.0%
	GTTAAGTTCATTCGTGTTTA+TGG	+	chr2.4:32115508-32115527	MS.gene77149:intron	30.0%
	TCATCAAATATGTACAGGTA+TGG	-	chr2.4:32116607-32116626	None:intergenic	30.0%
	TTAACAGCAACAATGATGAA+GGG	-	chr2.4:32116214-32116233	None:intergenic	30.0%
	TTCTAGATCATAATGTTCAC+TGG	+	chr2.4:32116127-32116146	MS.gene77149:intron	30.0%
	TTGAATAAAATACCTTGTCC+TGG	-	chr2.4:32115778-32115797	None:intergenic	30.0%
	TTGCAAATCAAAATTCACAC+AGG	-	chr2.4:32115015-32115034	None:intergenic	30.0%
!	TCTGAGATTTGAATTTACCT+TGG	-	chr2.4:32114303-32114322	None:intergenic	30.0%
!	TTCGATTTGCATGAAGAAAT+TGG	-	chr2.4:32114458-32114477	None:intergenic	30.0%
!	TTGCAAGTTTTGCTTATGTA+TGG	+	chr2.4:32115029-32115048	MS.gene77149:intron	30.0%
!!	AATCGATTTTCACTGTTGAT+CGG	-	chr2.4:32114577-32114596	None:intergenic	30.0%
!!	ATATTTGATGATGACTCTGA+TGG	+	chr2.4:32116616-32116635	MS.gene77149:CDS	30.0%
!!	TAGTTTTACAAATGCCAATC+AGG	-	chr2.4:32115181-32115200	None:intergenic	30.0%
!!!	ACTATCGATTTTATCCTGAT+TGG	+	chr2.4:32115164-32115183	MS.gene77149:intron	30.0%
	AAGTTCATTCGTGTTTATGG+AGG	+	chr2.4:32115511-32115530	MS.gene77149:intron	35.0%
	ACAAATATAAGACCTCCACA+AGG	-	chr2.4:32115869-32115888	None:intergenic	35.0%
	ACTGAACGATGAAAAACAGA+AGG	-	chr2.4:32114509-32114528	None:intergenic	35.0%
	ATCTAAACAGATCGAACGAA+TGG	-	chr2.4:32114486-32114505	None:intergenic	35.0%
	ATGAGAGGTGATACTTCAAA+AGG	-	chr2.4:32113796-32113815	None:intergenic	35.0%
	ATTCAACTATTCCTTGCTTC+AGG	+	chr2.4:32116469-32116488	MS.gene77149:CDS	35.0%
	CAAATATAAGACCTCCACAA+GGG	-	chr2.4:32115868-32115887	None:intergenic	35.0%
	CCTACTATTGGTAAGTTTCT+CGG	+	chr2.4:32114942-32114961	MS.gene77149:intron	35.0%
	GCAGATAAAAGAACATGCAA+AGG	-	chr2.4:32113684-32113703	None:intergenic	35.0%
	GCGGTTTATATTCAACGATT+AGG	+	chr2.4:32116514-32116533	MS.gene77149:CDS	35.0%
	GGATATTGAAGCTTATGATG+CGG	+	chr2.4:32116495-32116514	MS.gene77149:CDS	35.0%
	TAACATTGAACGTGATCAAG+TGG	+	chr2.4:32115277-32115296	MS.gene77149:intron	35.0%
	TGCTTGTACAACTCTGATTT+GGG	+	chr2.4:32116085-32116104	MS.gene77149:intron	35.0%
	TTGCTTGTACAACTCTGATT+TGG	+	chr2.4:32116084-32116103	MS.gene77149:intron	35.0%
!	AGAACATTAGCTTCACTGTT+TGG	+	chr2.4:32115741-32115760	MS.gene77149:intron	35.0%
!	CAAATGAGAGGCATAAGTTA+TGG	+	chr2.4:32114095-32114114	MS.gene77149:intron	35.0%
!	CATCAACTTTTCTTCTTCCA+AGG	+	chr2.4:32114283-32114302	MS.gene77149:intron	35.0%
!	GAACATTAGCTTCACTGTTT+GGG	+	chr2.4:32115742-32115761	MS.gene77149:intron	35.0%
!!	TCTGTCTTTTGGTAGTTGAA+TGG	+	chr2.4:32116285-32116304	MS.gene77149:intron	35.0%
	AAAGACAAAAAGGAGGAGAG+GGG	-	chr2.4:32115845-32115864	None:intergenic	40.0%
	AACGTGGAATAGGAATTGTG+GGG	-	chr2.4:32114344-32114363	None:intergenic	40.0%
	AAGCTTGAGCTTGTAGAGAA+TGG	-	chr2.4:32114901-32114920	None:intergenic	40.0%
	AAGGAAGTGAAGGAGAAAAC+GGG	+	chr2.4:32114786-32114805	MS.gene77149:CDS	40.0%
	AATTTCTGTCGAGCTTCTCA+TGG	+	chr2.4:32116179-32116198	MS.gene77149:CDS	40.0%
	ACAAGGGAAAGACAAAAAGG+AGG	-	chr2.4:32115852-32115871	None:intergenic	40.0%
	ACACGTGTCCAATTATCTGA+TGG	-	chr2.4:32114164-32114183	None:intergenic	40.0%
	ACTATTCCTTGCTTCAGGAT+TGG	+	chr2.4:32116474-32116493	MS.gene77149:CDS	40.0%
	AGGTTTGATCAAGGAAGTGA+AGG	+	chr2.4:32114776-32114795	MS.gene77149:intron	40.0%
	ATTCTCTACAAGCTCAAGCT+TGG	+	chr2.4:32114900-32114919	MS.gene77149:CDS	40.0%
	ATTTCTGTCGAGCTTCTCAT+GGG	+	chr2.4:32116180-32116199	MS.gene77149:CDS	40.0%
	CAACCATATCTACACACTGT+TGG	+	chr2.4:32113636-32113655	MS.gene77149:CDS	40.0%
	CAACGTGGAATAGGAATTGT+GGG	-	chr2.4:32114345-32114364	None:intergenic	40.0%
	CAAGGAAGTGAAGGAGAAAA+CGG	+	chr2.4:32114785-32114804	MS.gene77149:CDS	40.0%
	CAATATCCAATCCTGAAGCA+AGG	-	chr2.4:32116483-32116502	None:intergenic	40.0%
	CACCTGTTGTGTTTAGACAT+CGG	+	chr2.4:32115104-32115123	MS.gene77149:intron	40.0%
	CAGTGTGTAGATATGGTTGA+AGG	-	chr2.4:32113635-32113654	None:intergenic	40.0%
	CCGAGAAACTTACCAATAGT+AGG	-	chr2.4:32114945-32114964	None:intergenic	40.0%
	CTTACCAATAGTAGGAATGG+TGG	-	chr2.4:32114937-32114956	None:intergenic	40.0%
	GAAAGACAAAAAGGAGGAGA+GGG	-	chr2.4:32115846-32115865	None:intergenic	40.0%
	GAAATGCGAATTCTGATGGT+TGG	+	chr2.4:32114852-32114871	MS.gene77149:CDS	40.0%
	GAAGGAAATGCGAATTCTGA+TGG	+	chr2.4:32114848-32114867	MS.gene77149:CDS	40.0%
	GCTACATAGGATGTTGAATG+AGG	+	chr2.4:32115934-32115953	MS.gene77149:CDS	40.0%
	GGAGAATGAAGTATGATGAG+AGG	-	chr2.4:32113811-32113830	None:intergenic	40.0%
	GGTTGTGATTAACTCATGCA+TGG	-	chr2.4:32114143-32114162	None:intergenic	40.0%
	TAGCCAACAGTGTGTAGATA+TGG	-	chr2.4:32113642-32113661	None:intergenic	40.0%
	TCCACAAGGGAAAGACAAAA+AGG	-	chr2.4:32115855-32115874	None:intergenic	40.0%
	TCTCTAATCTCTGAATCAGG+AGG	+	chr2.4:32116375-32116394	MS.gene77149:intron	40.0%
	TGATCTCAGAAGAACGTTGT+TGG	-	chr2.4:32113747-32113766	None:intergenic	40.0%
	TGTCAGTCTCTAAATCAGCT+AGG	+	chr2.4:32116407-32116426	MS.gene77149:CDS	40.0%
	TGTCGTTGACAGTAATGACA+GGG	+	chr2.4:32115886-32115905	MS.gene77149:CDS	40.0%
	TTGTCGTTGACAGTAATGAC+AGG	+	chr2.4:32115885-32115904	MS.gene77149:CDS	40.0%
!	TCCTTTTTGTCTTTCCCTTG+TGG	+	chr2.4:32115851-32115870	MS.gene77149:CDS	40.0%
!	TTTTTGTCTTTCCCTTGTGG+AGG	+	chr2.4:32115854-32115873	MS.gene77149:CDS	40.0%
	AAATCAGCTAGGCCTTTCCT+GGG	+	chr2.4:32116418-32116437	MS.gene77149:CDS	45.0%
	AACGCGGAATAGGAATTGTG+GGG	-	chr2.4:32114213-32114232	None:intergenic	45.0%
	AAGAATGCTTTGCAGCAACG+CGG	-	chr2.4:32114229-32114248	None:intergenic	45.0%
	AAGAATGCTTTGCAGCAACG+TGG	-	chr2.4:32114229-32114248	None:intergenic	45.0%
	AAGACAAAAAGGAGGAGAGG+GGG	-	chr2.4:32115844-32115863	None:intergenic	45.0%
	AGGAAGTGAAGGAGAAAACG+GGG	+	chr2.4:32114787-32114806	MS.gene77149:CDS	45.0%
	AGGAATTGTGGGGTATGAGT+TGG	-	chr2.4:32114203-32114222	None:intergenic	45.0%
	ATCGCAAAGACAGAGAGAGA+AGG	-	chr2.4:32114255-32114274	None:intergenic	45.0%
	CAACGCGGAATAGGAATTGT+GGG	-	chr2.4:32114214-32114233	None:intergenic	45.0%
	CACACGTTTGGTCTAAGTGT+CGG	+	chr2.4:32115128-32115147	MS.gene77149:intron	45.0%
	CAGTCGGTTATTTGCGAAGA+AGG	+	chr2.4:32114830-32114849	MS.gene77149:CDS	45.0%
	CATCCTATGTAGCTCATCTC+TGG	-	chr2.4:32115927-32115946	None:intergenic	45.0%
	CATTCACGAAGCTGTTCAGT+CGG	+	chr2.4:32114814-32114833	MS.gene77149:CDS	45.0%
	CTTGAGCTTGTAGAGAATGG+TGG	-	chr2.4:32114898-32114917	None:intergenic	45.0%
	GCAACGTGGAATAGGAATTG+TGG	-	chr2.4:32114346-32114365	None:intergenic	45.0%
	GGAAAGACAAAAAGGAGGAG+AGG	-	chr2.4:32115847-32115866	None:intergenic	45.0%
	GTCACCACCATTCCTACTAT+TGG	+	chr2.4:32114930-32114949	MS.gene77149:CDS	45.0%
	GTCCGATGTCTAAACACAAC+AGG	-	chr2.4:32115109-32115128	None:intergenic	45.0%
	TAAATCAGCTAGGCCTTTCC+TGG	+	chr2.4:32116417-32116436	MS.gene77149:CDS	45.0%
	TCGCAAAGACAGAGAGAGAA+GGG	-	chr2.4:32114254-32114273	None:intergenic	45.0%
	TGACAGGGATAGAGTTGTTG+AGG	+	chr2.4:32115901-32115920	MS.gene77149:CDS	45.0%
	TTAGACATCGGACACACGTT+TGG	+	chr2.4:32115116-32115135	MS.gene77149:intron	45.0%
!	AGCTTCACTGTTTGGGATGT+TGG	+	chr2.4:32115749-32115768	MS.gene77149:intron	45.0%
!	GAAACATCAGTGTTTTCCCC+TGG	-	chr2.4:32116550-32116569	None:intergenic	45.0%
!	GTGGGGTATGAGTTGGAAAT+TGG	-	chr2.4:32114327-32114346	None:intergenic	45.0%
!	TTCACTGTTTGGGATGTTGG+AGG	+	chr2.4:32115752-32115771	MS.gene77149:intron	45.0%
!!	ACGTTCTTCTGAGATCAAGC+AGG	+	chr2.4:32113750-32113769	MS.gene77149:intron	45.0%
	AGACAAAAAGGAGGAGAGGG+GGG	-	chr2.4:32115843-32115862	None:intergenic	50.0%
	AGGCCAGAGATGAGCTACAT+AGG	+	chr2.4:32115921-32115940	MS.gene77149:CDS	50.0%
	AGGGTTCAATGTGGAGACTG+TGG	+	chr2.4:32115712-32115731	MS.gene77149:intron	50.0%
	ATTAGGATGGAGCTCACCAG+GGG	+	chr2.4:32116531-32116550	MS.gene77149:CDS	50.0%
	GATTAGGATGGAGCTCACCA+GGG	+	chr2.4:32116530-32116549	MS.gene77149:CDS	50.0%
	GCAACGCGGAATAGGAATTG+TGG	-	chr2.4:32114215-32114234	None:intergenic	50.0%
	GCTTTGCAGCAACGTGGAAT+AGG	-	chr2.4:32114354-32114373	None:intergenic	50.0%
	GGACACGTGTAATGATCCTG+TGG	+	chr2.4:32114174-32114193	MS.gene77149:intron	50.0%
	TGGTTCAATGCGTGTCTCTG+TGG	+	chr2.4:32116147-32116166	MS.gene77149:CDS	50.0%
!	GGGTATGAGTTGAGAACCAC+AGG	-	chr2.4:32114193-32114212	None:intergenic	50.0%
!!	ATTTGTCGGTTCTTGCTCCC+AGG	-	chr2.4:32116438-32116457	None:intergenic	50.0%
!!	TGCAAGAGTGCTGCGTAGAA+AGG	+	chr2.4:32115637-32115656	MS.gene77149:intron	50.0%
	CGATTAGGATGGAGCTCACC+AGG	+	chr2.4:32116529-32116548	MS.gene77149:CDS	55.0%
	GACAAAAAGGAGGAGAGGGG+GGG	-	chr2.4:32115842-32115861	None:intergenic	55.0%
	GCTTTGCAGCAACGCGGAAT+AGG	-	chr2.4:32114223-32114242	None:intergenic	55.0%
	TCGGTTCTTGCTCCCAGGAA+AGG	-	chr2.4:32116433-32116452	None:intergenic	55.0%
!	TGTTTGGGATGTTGGAGGCC+AGG	+	chr2.4:32115757-32115776	MS.gene77149:intron	55.0%
!!	ATGGTTGGTCTCGATGCAGC+TGG	+	chr2.4:32114867-32114886	MS.gene77149:CDS	55.0%
	GGATGTTGGAGGCCAGGACA+AGG	+	chr2.4:32115763-32115782	MS.gene77149:intron	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.4	gene	32113628	32116648	32113628	ID=MS.gene77149
chr2.4	mRNA	32113628	32116648	32113628	ID=MS.gene77149.t1;Parent=MS.gene77149
chr2.4	exon	32113628	32113661	32113628	ID=MS.gene77149.t1.exon1;Parent=MS.gene77149.t1
chr2.4	CDS	32113628	32113661	32113628	ID=cds.MS.gene77149.t1;Parent=MS.gene77149.t1
chr2.4	exon	32114778	32114951	32114778	ID=MS.gene77149.t1.exon2;Parent=MS.gene77149.t1
chr2.4	CDS	32114778	32114951	32114778	ID=cds.MS.gene77149.t1;Parent=MS.gene77149.t1
chr2.4	exon	32115837	32115955	32115837	ID=MS.gene77149.t1.exon3;Parent=MS.gene77149.t1
chr2.4	CDS	32115837	32115955	32115837	ID=cds.MS.gene77149.t1;Parent=MS.gene77149.t1
chr2.4	exon	32116133	32116228	32116133	ID=MS.gene77149.t1.exon4;Parent=MS.gene77149.t1
chr2.4	CDS	32116133	32116228	32116133	ID=cds.MS.gene77149.t1;Parent=MS.gene77149.t1
chr2.4	exon	32116394	32116648	32116394	ID=MS.gene77149.t1.exon5;Parent=MS.gene77149.t1
chr2.4	CDS	32116394	32116648	32116394	ID=cds.MS.gene77149.t1;Parent=MS.gene77149.t1

Gene Sequence

>MS.gene77149

ATGTCCTTCAACCATATCTACACACTGTTGGCTAGTGAGAAGAGTTTGTTATACCTTTGCATGTTCTTTTATCTGCTCTCCTAATTGAAATCTTCTATTGATCATACAATTTGTTTCCAACAACGTTCTTCTGAGATCAAGCAGGATATATTCCAATTTTGATTTCCTTTTGAAGTATCACCTCTCATCATACTTCATTCTCCTGAAGACATTCTTATGATTTGTTCTAAGACATCTTTTCAAGATTTTTCTCTAGCTTTGATATGTAATATAAATCCTTTTGTTAGCATATATGTTGTGCACTGATTCCTCAACTTTATTAGTTGTAGAATGATGCGCAATGTATATCTAGTTTATTTCTCTTAAGAGAATCACATGTTATTCTTGAGAAATTATCCAATCTTTATATGTTGTGATTATTAACCATGATTAGTCCATATTTTTTTTTTATTTGTACAAAGTACATACAAATGAGAGGCATAAGTTATGGTGCATAAGTCATAACTTATGCACCATGCATGAGTTAATCACAACCATCAGATAATTGGACACGTGTAATGATCCTGTGGTTCTCAACTCATACCCCACAATTCCTATTCCGCGTTGCTGCAAAGCATTCTTCACCCTTCTCTCTCTGTCTTTGCGATTTCTGCTACATCAACTTTTCTTCTTCCAAGGTAAATTCAAATCTCAGATCCAATTTCCAACTCATACCCCACAATTCCTATTCCACGTTGCTGCAAAGCATTCTTCACCCTTCTCTCTCTGTCTTTGCGATTTCTGCTACATCAACTTTTCTTCTTCCAAGGTAAATTCAAATCTCAGATCCAATTTCTTCATGCAAATCGAATCTTTCCATTCGTTCGATCTGTTTAGATCCTTCTGTTTTTCATCGTTCAGTTAATTTTCGTCTTTTTATCGATTTTTTCATTTGATCTTGTGCGATCCGATCAACAGTGAAAATCGATTTTGCATGCTACTTTCTTTAATATTTTCTCGTTTAGCGTGAGATCTGATTGAATCTGATGATATCAATGCAATTTATTGATGTATTAGCGTTTTGATTTAGGATTTAATTGATTTGCGCAAGAAATTATGAATAATGTTTATGTTTTGGAGATTTGTTATTGAAATTTTGATTGAAATTTAGGTTTGATCAAGGAAGTGAAGGAGAAAACGGGGTTAACATTCACGAAGCTGTTCAGTCGGTTATTTGCGAAGAAGGAAATGCGAATTCTGATGGTTGGTCTCGATGCAGCTGGTAAGACCACCATTCTCTACAAGCTCAAGCTTGGAGAAATTGTCACCACCATTCCTACTATTGGTAAGTTTCTCGGTTCGACTTTTGTTGTTAGCTAGATTCATATATCATAATTGTTATTTACCTGTGTGAATTTTGATTTGCAAGTTTTGCTTATGTATGGACATGTTGACACTGATAATTATTTGAAAAAATTACATAATTGAATGCAATCACACCTGTTGTGTTTAGACATCGGACACACGTTTGGTCTAAGTGTCGGTGCTATGTAGTTCACTATCGATTTTATCCTGATTGGCATTTGTAAAACTATATGTAATGTATGTATTGATTGCTTTGGTCTTTTGCTATATAACCGACTTCTTTGCATGTTAATTCAATGATTGTTTAACATTGAACGTGATCAAGTGGTCAACACATTGTTAAATCTGTGAAGAAGTTTGCCTTTATATTTAACAATGAAATCCCAAACTTTATGTCATATATTGTTTAACATGTGTGATTGATGTTAGACGTGCAAATATTGTTAAACAACTATAATGACTTAGTTTGGATTAGGTTCTATCTTACAAGAGCTTCTCAATTGAAATATGCATTTGGTTTGTAGAACATAATTTTTGTTAAGTTCATTCGTGTTTATGGAGGAGTTACACTTACCATGTAATTTCATCCATGTTTATTTTAAACTTGATGGGAAACTTTATTTAAAATACTCATTATGATCAAAAGATTATTCTAATTTTATTTTTGCAAGAGTGCTGCGTAGAAAGGTTGAAATGCTGTTATTTGTATTCATTAATTTATTTATTTTATATTTTAATTTAGGGTTCAATGTGGAGACTGTGGAATACAAGAACATTAGCTTCACTGTTTGGGATGTTGGAGGCCAGGACAAGGTATTTTATTCAAATTCATTTTGAAAATAGTGTACTTCACAAGATATTTTAGCACCCCCCCTCTCCTCCTTTTTGTCTTTCCCTTGTGGAGGTCTTATATTTGTCGTTGACAGTAATGACAGGGATAGAGTTGTTGAGGCCAGAGATGAGCTACATAGGATGTTGAATGAGGTACTTTTCTTATATTCAGTTTGGCATGTTGTGCTATATTTTCTTCGTATTTTTTTTCTTTCCATCCACAAATTTGTTGAGAATTGAGATAGAAAGATATAGGAATCAAATTGATTTATGAAGATGCGTTGCTTGTACAACTCTGATTTGGGTAGAGATACTGATACATGATTCTAGATCATAATGTTCACTGGTTCAATGCGTGTCTCTGTGGATGAAGATGAATTTCTGTCGAGCTTCTCATGGGAAGCATTTCCCTTCATCATTGTTGCTGTTAATTAATCCATGTTTTTGCTGGATCATATTTTCTGATGACTGCTGTATATTAATCTGTCTTTTGGTAGTTGAATGGTCAGTTTTCTGATACTGATATAATCGTATAACATTATATGAATATGAAAAAAGTGATGTATGTTAATTCTCTAATCTCTGAATCAGGAGGAGTTCATGATGTCAGTCTCTAAATCAGCTAGGCCTTTCCTGGGAGCAAGAACCGACAAATTTGTGTATGAGATTCAACTATTCCTTGCTTCAGGATTGGATATTGAAGCTTATGATGCGGTTTATATTCAACGATTAGGATGGAGCTCACCAGGGGAAAACACTGATGTTTCTCAAAATGAATTGGTTGATCGCACTACTGTGATTCCATACCTGTACATATTTGATGATGACTCTGATGGAAATGAATAG

Protein sequence

>MS.gene77149.t1

MSFNHIYTLLASLIKEVKEKTGLTFTKLFSRLFAKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIAPPLSSFLSFPCGGLIFVVDSNDRDRVVEARDELHRMLNEIIMFTGSMRVSVDEDEFLSSFSWEAFPFIIVAEEFMMSVSKSARPFLGARTDKFVYEIQLFLASGLDIEAYDAVYIQRLGWSSPGENTDVSQNELVDRTTVIPYLYIFDDDSDGNE