AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene054179

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene054179.t1 MTR_6g047800 96.333 300 10 1 1 299 1 300 0.0 592
MS.gene054179.t1 MTR_6g047800 96.220 291 10 1 10 299 1 291 0.0 574
MS.gene054179.t1 MTR_2g014590 77.922 231 51 0 1 231 2 232 8.36e-137 386
MS.gene054179.t1 MTR_2g014570 64.815 54 14 3 246 299 6 54 2.17e-11 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene054179.t1 AT5G14600 74.183 306 72 2 1 299 1 306 1.51e-170 475

Find 62 sgRNAs with CRISPR-Local

Find 188 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGCTTTGTTGTTATGTATT+TGG 0.132518 6.1:+28785769 MS.gene054179:CDS
TCCTGAAGAGTTTGCTGGCT+TGG 0.242918 6.1:+28787396 MS.gene054179:CDS
TAGCTCCTACTCCTGAATTA+TGG 0.261887 6.1:+28785699 MS.gene054179:CDS
TTCTAATTCTTCTTCTGTTA+TGG 0.267894 6.1:+28787849 MS.gene054179:CDS
AACAATTAGTAACGGTGATT+TGG 0.271982 6.1:+28785520 MS.gene054179:CDS
GGTTGTGTCGTTCTTGAATC+TGG 0.284355 6.1:+28785803 MS.gene054179:CDS
CGAAGTTCGAGAAGGGAAAA+TGG 0.297664 6.1:+28787735 MS.gene054179:CDS
GTCTAAAGTATTCAACAATA+AGG 0.308450 6.1:+28785658 MS.gene054179:CDS
TTGTTTATGTGAAAGATATA+AGG 0.311677 6.1:+28787686 MS.gene054179:intron
GATTGGATAGGAAAGTCATT+TGG 0.331486 6.1:+28785635 MS.gene054179:CDS
AAAACTAAAGTCCATAATTC+AGG 0.343342 6.1:-28785710 None:intergenic
CTTGAATCTGGCACTGGAAG+TGG 0.346516 6.1:+28785815 MS.gene054179:CDS
GCCAAGCCAGCAAACTCTTC+AGG 0.354828 6.1:-28787397 None:intergenic
ATGTATTTGGAGATTGTTCC+TGG 0.385811 6.1:+28785782 MS.gene054179:CDS
GACGGTAATGACGGCTCTAA+TGG 0.408113 6.1:+28787772 MS.gene054179:CDS
GATCCCTCAGATACTGTTAC+TGG 0.419969 6.1:-28785575 None:intergenic
ATTGGATAGGAAAGTCATTT+GGG 0.422230 6.1:+28785636 MS.gene054179:CDS
CGGGAAAGTACCGGTGAAGT+TGG 0.459909 6.1:-28787557 None:intergenic
TCTAAAGTATTCAACAATAA+GGG 0.468151 6.1:+28785659 MS.gene054179:CDS
TTGAATCTGGCACTGGAAGT+GGG 0.495210 6.1:+28785816 MS.gene054179:CDS
GTTGTACTTGTTCAATGCAC+GGG 0.495893 6.1:-28787513 None:intergenic
GTCGTTCTTGAATCTGGCAC+TGG 0.503209 6.1:+28785809 MS.gene054179:CDS
CTTTAACTACTTCACTTGCA+AGG 0.505822 6.1:+28785840 MS.gene054179:CDS
ATTTGGCAATTGTTTATGAA+AGG 0.507354 6.1:+28785537 MS.gene054179:CDS
ATGGAAAGCTCTGAGATTGA+CGG 0.512304 6.1:+28787754 MS.gene054179:CDS
TTTAAACATTCAGATTGGAT+AGG 0.513874 6.1:+28785623 MS.gene054179:CDS
GCAAGGGCAGTTGCTCCTAC+AGG 0.518867 6.1:+28785857 MS.gene054179:CDS
CGTTGTACTTGTTCAATGCA+CGG 0.540755 6.1:-28787514 None:intergenic
AAAGTCCATAATTCAGGAGT+AGG 0.545264 6.1:-28785704 None:intergenic
ATAAGCAGCTTAGTAACTGC+AGG 0.549586 6.1:+28787346 MS.gene054179:CDS
GCAGGAGTGAGGGACATTCA+AGG 0.551907 6.1:+28787364 MS.gene054179:CDS
GCTTAGTAACTGCAGGAGTG+AGG 0.562608 6.1:+28787353 MS.gene054179:CDS
GAACGTTGCCTCCTCTTGCA+AGG 0.567462 6.1:-28787802 None:intergenic
GGAATAGCTAGCCAAGGTTG+AGG 0.572196 6.1:-28787442 None:intergenic
GTGAGGGACATTCAAGGTGA+GGG 0.573477 6.1:+28787370 MS.gene054179:CDS
ATGGTTCTCTTCCTTGCAAG+AGG 0.575002 6.1:+28787791 MS.gene054179:CDS
TTTAACTACTTCACTTGCAA+GGG 0.578833 6.1:+28785841 MS.gene054179:CDS
GAAGCCAGTAACAGTATCTG+AGG 0.579372 6.1:+28785571 MS.gene054179:CDS
ACAATGCAATCCAACTTCAC+CGG 0.584109 6.1:+28787547 MS.gene054179:CDS
GTTCTCTTCCTTGCAAGAGG+AGG 0.584259 6.1:+28787794 MS.gene054179:CDS
GATTCAAGAACGACACAACC+AGG 0.585429 6.1:-28785800 None:intergenic
AGTAACAGTATCTGAGGGAT+CGG 0.586054 6.1:+28785577 MS.gene054179:CDS
TCTGAGATTGACGGTAATGA+CGG 0.588128 6.1:+28787763 MS.gene054179:CDS
CTTAGTAACTGCAGGAGTGA+GGG 0.595594 6.1:+28787354 MS.gene054179:CDS
TTTAATAGAACAATTAGTAA+CGG 0.596180 6.1:+28785512 MS.gene054179:CDS
AAGAGGAGGCAACGTTCAGA+TGG 0.606722 6.1:+28787808 MS.gene054179:CDS
AACGTATAAACATGTCCTGT+AGG 0.611682 6.1:-28785872 None:intergenic
CGGACATACGAAGTTCGAGA+AGG 0.611846 6.1:+28787727 MS.gene054179:CDS
CTCGAACTTCGTATGTCCGT+AGG 0.612530 6.1:-28787723 None:intergenic
TGACCTCTAGCTTCACCACA+TGG 0.618356 6.1:-28787877 None:intergenic
ACGTTCAGATGGAGCTAGCG+TGG 0.623569 6.1:+28787819 MS.gene054179:CDS
AGTGAGGGACATTCAAGGTG+AGG 0.624772 6.1:+28787369 MS.gene054179:CDS
GGACATACGAAGTTCGAGAA+GGG 0.625372 6.1:+28787728 MS.gene054179:CDS
TCTGTTATGGCTAGACCATG+TGG 0.628693 6.1:+28787862 MS.gene054179:CDS
AACAAAGATCTGGATCAGCT+AGG 0.636973 6.1:+28785906 MS.gene054179:CDS
AGACCATGTGGTGAAGCTAG+AGG 0.638065 6.1:+28787874 MS.gene054179:CDS
AAAGTATTCAACAATAAGGG+CGG 0.646223 6.1:+28785662 MS.gene054179:CDS
TATATAGAATCTGTGTCCTG+TGG 0.646737 6.1:-28785736 None:intergenic
GCTGAAGGAATAGCTAGCCA+AGG 0.647664 6.1:-28787448 None:intergenic
AAGCCAGTAACAGTATCTGA+GGG 0.661239 6.1:+28785572 MS.gene054179:CDS
TTGTACTTGTTCAATGCACG+GGG 0.675813 6.1:-28787512 None:intergenic
GGTGAAGCTAGAGGTCACAC+TGG 0.683965 6.1:+28787883 MS.gene054179:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGTCAATTATATATAAAACA+AGG - chr6.1:28786982-28787001 None:intergenic 15.0%
!! GTAACTTATATATTGTATTA+CGG - chr6.1:28786921-28786940 None:intergenic 15.0%
!! TTTAATAGAACAATTAGTAA+CGG + chr6.1:28785512-28785531 MS.gene054179:CDS 15.0%
!!! TTTTTACAATGAAAAAACTT+AGG + chr6.1:28786868-28786887 MS.gene054179:intron 15.0%
!! AAATAAGATACGTATAAAAG+AGG - chr6.1:28787588-28787607 None:intergenic 20.0%
!! ACATAAACAAACATTTACAA+AGG - chr6.1:28787676-28787695 None:intergenic 20.0%
!! TTGTTTATGTGAAAGATATA+AGG + chr6.1:28787686-28787705 MS.gene054179:intron 20.0%
!!! TCTAAAGTATTCAACAATAA+GGG + chr6.1:28785659-28785678 MS.gene054179:CDS 20.0%
! AAAACTAAAGTCCATAATTC+AGG - chr6.1:28785713-28785732 None:intergenic 25.0%
! ATATACATGTGCTTATCTTT+AGG + chr6.1:28785963-28785982 MS.gene054179:intron 25.0%
! ATTTGGCAATTGTTTATGAA+AGG + chr6.1:28785537-28785556 MS.gene054179:CDS 25.0%
! CTACCAAAAAACTAGAATAA+CGG - chr6.1:28786093-28786112 None:intergenic 25.0%
! TACGTATAAAAGAGGAAAAT+CGG - chr6.1:28787580-28787599 None:intergenic 25.0%
! TTCTAATTCTTCTTCTGTTA+TGG + chr6.1:28787849-28787868 MS.gene054179:CDS 25.0%
! TTTAAACATTCAGATTGGAT+AGG + chr6.1:28785623-28785642 MS.gene054179:CDS 25.0%
! TTTCTGTAATATGCTATTGA+TGG + chr6.1:28787067-28787086 MS.gene054179:intron 25.0%
!! GTCTAAAGTATTCAACAATA+AGG + chr6.1:28785658-28785677 MS.gene054179:CDS 25.0%
!! TAATTGTACTCAACTTTTGT+TGG + chr6.1:28787200-28787219 MS.gene054179:intron 25.0%
!!! GAGAATGATTTTTTCAATAG+CGG + chr6.1:28786650-28786669 MS.gene054179:intron 25.0%
!!! GTGTTTTTAAACATTCAGAT+TGG + chr6.1:28785618-28785637 MS.gene054179:CDS 25.0%
AAAATCACGATCAAACTGAT+GGG - chr6.1:28786406-28786425 None:intergenic 30.0%
AACAATTAGTAACGGTGATT+TGG + chr6.1:28785520-28785539 MS.gene054179:CDS 30.0%
ACGTATAAAAGAGGAAAATC+GGG - chr6.1:28787579-28787598 None:intergenic 30.0%
ACTGTAATGGTGTGTTTATA+TGG - chr6.1:28786188-28786207 None:intergenic 30.0%
ATTGGATAGGAAAGTCATTT+GGG + chr6.1:28785636-28785655 MS.gene054179:CDS 30.0%
ATTGTAAAAATAAGCCACGT+TGG - chr6.1:28786858-28786877 None:intergenic 30.0%
CTATCTTCTATTTCACATAG+CGG + chr6.1:28787042-28787061 MS.gene054179:intron 30.0%
GACTTATTTCAACTGTTATG+CGG + chr6.1:28786998-28787017 MS.gene054179:intron 30.0%
GCAAAAATGTTACGATATGA+CGG + chr6.1:28787472-28787491 MS.gene054179:CDS 30.0%
GCTGATATAGGAACTATATT+GGG + chr6.1:28786781-28786800 MS.gene054179:intron 30.0%
TATGTAATATTGCATCAGCT+AGG + chr6.1:28786569-28786588 MS.gene054179:intron 30.0%
TATTGTATTACGGTGAATAC+AGG - chr6.1:28786911-28786930 None:intergenic 30.0%
TGAAATAGAAGATAGTGTTG+CGG - chr6.1:28787037-28787056 None:intergenic 30.0%
TTGTAAAAATAAGCCACGTT+GGG - chr6.1:28786857-28786876 None:intergenic 30.0%
TTTAACTACTTCACTTGCAA+GGG + chr6.1:28785841-28785860 MS.gene054179:CDS 30.0%
TTTCTCGTTCTAGTAATTGT+AGG + chr6.1:28787117-28787136 MS.gene054179:intron 30.0%
! AAAGTATTCAACAATAAGGG+CGG + chr6.1:28785662-28785681 MS.gene054179:CDS 30.0%
! AGACAAAAGAGAATTTTAGG+CGG + chr6.1:28786132-28786151 MS.gene054179:intron 30.0%
! AGCAGACAAAAGAGAATTTT+AGG + chr6.1:28786129-28786148 MS.gene054179:intron 30.0%
! ATACGATTTTCAGACTGTAA+TGG - chr6.1:28786201-28786220 None:intergenic 30.0%
! ATTTGATTTCCATGAGCATA+GGG + chr6.1:28786536-28786555 MS.gene054179:intron 30.0%
! CAGTCTGAAAATCGTATTTT+TGG + chr6.1:28786204-28786223 MS.gene054179:intron 30.0%
! GAAAGATATAAGGACTTTTG+AGG + chr6.1:28787696-28787715 MS.gene054179:intron 30.0%
! TTTCTAGTGTTGTCAACTAT+TGG + chr6.1:28786004-28786023 MS.gene054179:intron 30.0%
!! ATACTTGTTTTTCTCCTTGT+AGG + chr6.1:28787302-28787321 MS.gene054179:intron 30.0%
!! ATCTTGCAATTCATTGATTC+AGG - chr6.1:28786282-28786301 None:intergenic 30.0%
!! CAGCTTTGTTGTTATGTATT+TGG + chr6.1:28785769-28785788 MS.gene054179:CDS 30.0%
!! TACTTGTTTTTCTCCTTGTA+GGG + chr6.1:28787303-28787322 MS.gene054179:intron 30.0%
!!! AGTGTTGCAGTTTTTTTTAG+CGG - chr6.1:28786058-28786077 None:intergenic 30.0%
!!! TTGCCGTTATTCTAGTTTTT+TGG + chr6.1:28786087-28786106 MS.gene054179:intron 30.0%
AAAGTCCATAATTCAGGAGT+AGG - chr6.1:28785707-28785726 None:intergenic 35.0%
AACGTATAAACATGTCCTGT+AGG - chr6.1:28785875-28785894 None:intergenic 35.0%
ACACATGTATCAAACATCAG+AGG - chr6.1:28786610-28786629 None:intergenic 35.0%
ACCCTGCATGCAAATAAAAA+GGG - chr6.1:28786958-28786977 None:intergenic 35.0%
ACTTGAGATGTTCAATGCAA+AGG + chr6.1:28787263-28787282 MS.gene054179:intron 35.0%
ATAAACATGCCCTTCACAAA+TGG - chr6.1:28786514-28786533 None:intergenic 35.0%
ATCCCTTCATAAATTGGCTA+TGG + chr6.1:28786448-28786467 MS.gene054179:intron 35.0%
ATGTATTTGGAGATTGTTCC+TGG + chr6.1:28785782-28785801 MS.gene054179:CDS 35.0%
CAAAATCACGATCAAACTGA+TGG - chr6.1:28786407-28786426 None:intergenic 35.0%
CGTAGTAGTTATGCAACTAA+TGG + chr6.1:28787623-28787642 MS.gene054179:intron 35.0%
CTTTAACTACTTCACTTGCA+AGG + chr6.1:28785840-28785859 MS.gene054179:CDS 35.0%
GAAATAGAAGATAGTGTTGC+GGG - chr6.1:28787036-28787055 None:intergenic 35.0%
GATTGGATAGGAAAGTCATT+TGG + chr6.1:28785635-28785654 MS.gene054179:CDS 35.0%
GCATGCAAATAAAAAGGGAA+TGG - chr6.1:28786953-28786972 None:intergenic 35.0%
GGCTGATATAGGAACTATAT+TGG + chr6.1:28786780-28786799 MS.gene054179:intron 35.0%
GGTTATCTTCCTTGAAATTC+CGG - chr6.1:28786261-28786280 None:intergenic 35.0%
GTATGGTCGGAATCAAAATT+TGG - chr6.1:28786349-28786368 None:intergenic 35.0%
GTTATCTTCCTTGAAATTCC+GGG - chr6.1:28786260-28786279 None:intergenic 35.0%
TATATAGAATCTGTGTCCTG+TGG - chr6.1:28785739-28785758 None:intergenic 35.0%
TTCTCAAAATCATCCCTACA+AGG - chr6.1:28787319-28787338 None:intergenic 35.0%
! CATTTGATTTCCATGAGCAT+AGG + chr6.1:28786535-28786554 MS.gene054179:intron 35.0%
! GATTTTCACGAACAAAGATC+TGG + chr6.1:28785896-28785915 MS.gene054179:CDS 35.0%
! TCGGTTTTACAGAATCGATT+CGG + chr6.1:28785596-28785615 MS.gene054179:CDS 35.0%
!! AGGGATGATTTTGAGAAGAT+TGG + chr6.1:28787322-28787341 MS.gene054179:intron 35.0%
!! TCCCTTTTTATTTGCATGCA+GGG + chr6.1:28786954-28786973 MS.gene054179:intron 35.0%
!! TTCCCTTTTTATTTGCATGC+AGG + chr6.1:28786953-28786972 MS.gene054179:intron 35.0%
!!! ATCGTATTTTTGGTGAAACG+TGG + chr6.1:28786214-28786233 MS.gene054179:intron 35.0%
AACAAAGATCTGGATCAGCT+AGG + chr6.1:28785906-28785925 MS.gene054179:CDS 40.0%
AAGCCAGTAACAGTATCTGA+GGG + chr6.1:28785572-28785591 MS.gene054179:CDS 40.0%
ACAATGCAATCCAACTTCAC+CGG + chr6.1:28787547-28787566 MS.gene054179:CDS 40.0%
ACCGCAGAAAATTGTAGGTA+CGG - chr6.1:28786309-28786328 None:intergenic 40.0%
ACTATAGTGCTATCACATGG+TGG + chr6.1:28786674-28786693 MS.gene054179:intron 40.0%
AGTAACAGTATCTGAGGGAT+CGG + chr6.1:28785577-28785596 MS.gene054179:CDS 40.0%
ATAAGCAGCTTAGTAACTGC+AGG + chr6.1:28787346-28787365 MS.gene054179:CDS 40.0%
ATCAAACATCAGAGGAAGTC+AGG - chr6.1:28786602-28786621 None:intergenic 40.0%
CACCCTGCATGCAAATAAAA+AGG - chr6.1:28786959-28786978 None:intergenic 40.0%
CCTTCATAAATTGGCTATGG+CGG + chr6.1:28786451-28786470 MS.gene054179:intron 40.0%
CGCCATAGCCAATTTATGAA+GGG - chr6.1:28786453-28786472 None:intergenic 40.0%
CGTTGTACTTGTTCAATGCA+CGG - chr6.1:28787517-28787536 None:intergenic 40.0%
CTTCATAAATTGGCTATGGC+GGG + chr6.1:28786452-28786471 MS.gene054179:intron 40.0%
GGTCGGAATCAAAATTTGGT+GGG - chr6.1:28786345-28786364 None:intergenic 40.0%
GTTGTACTTGTTCAATGCAC+GGG - chr6.1:28787516-28787535 None:intergenic 40.0%
TAGCTCCTACTCCTGAATTA+TGG + chr6.1:28785699-28785718 MS.gene054179:CDS 40.0%
TGGTCGGAATCAAAATTTGG+TGG - chr6.1:28786346-28786365 None:intergenic 40.0%
TTGTACTTGTTCAATGCACG+GGG - chr6.1:28787515-28787534 None:intergenic 40.0%
! ACCGTACCTACAATTTTCTG+CGG + chr6.1:28786305-28786324 MS.gene054179:intron 40.0%
! CGTAACATTTTTGCAGCTGA+AGG - chr6.1:28787466-28787485 None:intergenic 40.0%
! TCTGAGATTGACGGTAATGA+CGG + chr6.1:28787763-28787782 MS.gene054179:CDS 40.0%
! TGCTATTGATGGCACTTTCA+TGG + chr6.1:28787078-28787097 MS.gene054179:intron 40.0%
! TTTTGGATCTACCTCAACCT+TGG + chr6.1:28787431-28787450 MS.gene054179:CDS 40.0%
!! ATGGAAAGCTCTGAGATTGA+CGG + chr6.1:28787754-28787773 MS.gene054179:CDS 40.0%
!! CGTATGATTTTGATACCCCT+TGG - chr6.1:28786381-28786400 None:intergenic 40.0%
!! CTATTGGCTCTATAGCATAG+CGG + chr6.1:28786020-28786039 MS.gene054179:intron 40.0%
!! GAAGTTTTGCTGGCTGATAT+AGG + chr6.1:28786769-28786788 MS.gene054179:intron 40.0%
!! TTTTTGGTGAAACGTGGTCA+TGG + chr6.1:28786220-28786239 MS.gene054179:intron 40.0%
!!! ATTTTAGGCGGTGTTGTCAA+TGG + chr6.1:28786144-28786163 MS.gene054179:intron 40.0%
!!! CTTGGCTGATGCAGTTTTTT+TGG + chr6.1:28787414-28787433 MS.gene054179:CDS 40.0%
!!! GATCGTGATTTTGACTTCCT+TGG + chr6.1:28786414-28786433 MS.gene054179:intron 40.0%
!!! GGACTTTAGTTTTAAGCCAC+AGG + chr6.1:28785720-28785739 MS.gene054179:CDS 40.0%
AACATGCCCTTCACAAATGG+TGG - chr6.1:28786511-28786530 None:intergenic 45.0%
ATGGTTCTCTTCCTTGCAAG+AGG + chr6.1:28787791-28787810 MS.gene054179:CDS 45.0%
CCGCCATAGCCAATTTATGA+AGG - chr6.1:28786454-28786473 None:intergenic 45.0%
CGAAGTTCGAGAAGGGAAAA+TGG + chr6.1:28787735-28787754 MS.gene054179:CDS 45.0%
CTTAGTAACTGCAGGAGTGA+GGG + chr6.1:28787354-28787373 MS.gene054179:CDS 45.0%
GAAGCCAGTAACAGTATCTG+AGG + chr6.1:28785571-28785590 MS.gene054179:CDS 45.0%
GATCCCTCAGATACTGTTAC+TGG - chr6.1:28785578-28785597 None:intergenic 45.0%
GATTCAAGAACGACACAACC+AGG - chr6.1:28785803-28785822 None:intergenic 45.0%
GCAGAAAATTGTAGGTACGG+TGG - chr6.1:28786306-28786325 None:intergenic 45.0%
GGACATACGAAGTTCGAGAA+GGG + chr6.1:28787728-28787747 MS.gene054179:CDS 45.0%
GGATCACCGCAGAAAATTGT+AGG - chr6.1:28786314-28786333 None:intergenic 45.0%
GGCACTATAGTGCTATCACA+TGG + chr6.1:28786671-28786690 MS.gene054179:intron 45.0%
TCCTTGAAATTCCGGGTTCA+CGG - chr6.1:28786253-28786272 None:intergenic 45.0%
TCTGTTATGGCTAGACCATG+TGG + chr6.1:28787862-28787881 MS.gene054179:CDS 45.0%
TGAGTCCACTATGAAAACCG+TGG + chr6.1:28786704-28786723 MS.gene054179:intron 45.0%
TGCTTGTATACACGTGTGTG+CGG + chr6.1:28787234-28787253 MS.gene054179:intron 45.0%
TTTCGCTATCAGGACAGTAG+CGG + chr6.1:28786738-28786757 MS.gene054179:intron 45.0%
! ACAATTTTCTGCGGTGATCC+TGG + chr6.1:28786314-28786333 MS.gene054179:intron 45.0%
! ACTGTCCTGATAGCGAAACT+GGG - chr6.1:28786736-28786755 None:intergenic 45.0%
! GAGGAAAATCGGGAAAGTAC+CGG - chr6.1:28787569-28787588 None:intergenic 45.0%
! GGCTGCTATTGAAGTTTTGC+TGG + chr6.1:28786759-28786778 MS.gene054179:intron 45.0%
! GGTTGTGTCGTTCTTGAATC+TGG + chr6.1:28785803-28785822 MS.gene054179:CDS 45.0%
! GGTTTTCCTGAAGAGTTTGC+TGG + chr6.1:28787391-28787410 MS.gene054179:CDS 45.0%
! TACTGTCCTGATAGCGAAAC+TGG - chr6.1:28786737-28786756 None:intergenic 45.0%
! TTGAATCTGGCACTGGAAGT+GGG + chr6.1:28785816-28785835 MS.gene054179:CDS 45.0%
!!! GATTTTGACTTCCTTGGCCA+TGG + chr6.1:28786420-28786439 MS.gene054179:intron 45.0%
!!! GGACTTTTGAGGTACTCCTA+CGG + chr6.1:28787707-28787726 MS.gene054179:CDS 45.0%
AAATTCCGGGTTCACGGGTT+GGG - chr6.1:28786247-28786266 None:intergenic 50.0%
AAGAGGAGGCAACGTTCAGA+TGG + chr6.1:28787808-28787827 MS.gene054179:CDS 50.0%
ACCATACTCCAGCATGCCAA+GGG + chr6.1:28786362-28786381 MS.gene054179:intron 50.0%
AGACCATGTGGTGAAGCTAG+AGG + chr6.1:28787874-28787893 MS.gene054179:CDS 50.0%
AGTGAGGGACATTCAAGGTG+AGG + chr6.1:28787369-28787388 MS.gene054179:CDS 50.0%
CCTTGAAATTCCGGGTTCAC+GGG - chr6.1:28786252-28786271 None:intergenic 50.0%
CGGACATACGAAGTTCGAGA+AGG + chr6.1:28787727-28787746 MS.gene054179:CDS 50.0%
CTCGAACTTCGTATGTCCGT+AGG - chr6.1:28787726-28787745 None:intergenic 50.0%
GATTTCCATGAGCATAGGGC+TGG + chr6.1:28786540-28786559 MS.gene054179:intron 50.0%
GCGGGCATCCCTTCATAAAT+TGG + chr6.1:28786442-28786461 MS.gene054179:intron 50.0%
GCTATCCCAGTTTCGCTATC+AGG + chr6.1:28786728-28786747 MS.gene054179:intron 50.0%
GCTGAAGGAATAGCTAGCCA+AGG - chr6.1:28787451-28787470 None:intergenic 50.0%
GCTTAGTAACTGCAGGAGTG+AGG + chr6.1:28787353-28787372 MS.gene054179:CDS 50.0%
GGAATAGCTAGCCAAGGTTG+AGG - chr6.1:28787445-28787464 None:intergenic 50.0%
GTCGTTCTTGAATCTGGCAC+TGG + chr6.1:28785809-28785828 MS.gene054179:CDS 50.0%
GTGAGGGACATTCAAGGTGA+GGG + chr6.1:28787370-28787389 MS.gene054179:CDS 50.0%
GTTCTCTTCCTTGCAAGAGG+AGG + chr6.1:28787794-28787813 MS.gene054179:CDS 50.0%
TTGGCATGCTGGAGTATGGT+CGG - chr6.1:28786362-28786381 None:intergenic 50.0%
TTTGATACCCCTTGGCATGC+TGG - chr6.1:28786373-28786392 None:intergenic 50.0%
! AAAATTTGGTGGGTGCAGCC+AGG - chr6.1:28786335-28786354 None:intergenic 50.0%
! CTTGAATCTGGCACTGGAAG+TGG + chr6.1:28785815-28785834 MS.gene054179:CDS 50.0%
! GACGGTAATGACGGCTCTAA+TGG + chr6.1:28787772-28787791 MS.gene054179:CDS 50.0%
! TAGCGCCACGGTTTTCATAG+TGG - chr6.1:28786712-28786731 None:intergenic 50.0%
! TGACCTCTAGCTTCACCACA+TGG - chr6.1:28787880-28787899 None:intergenic 50.0%
! TTTGACTTCCTTGGCCATGG+CGG + chr6.1:28786423-28786442 MS.gene054179:intron 50.0%
!! TCCTGAAGAGTTTGCTGGCT+TGG + chr6.1:28787396-28787415 MS.gene054179:CDS 50.0%
!! TTAGGCGGTGTTGTCAATGG+TGG + chr6.1:28786147-28786166 MS.gene054179:intron 50.0%
ACCGTGGCCACCATTTGTGA+AGG + chr6.1:28786501-28786520 MS.gene054179:intron 55.0%
ACGTTCAGATGGAGCTAGCG+TGG + chr6.1:28787819-28787838 MS.gene054179:CDS 55.0%
ATCTGCATGTGTGCCCAACG+TGG + chr6.1:28786841-28786860 MS.gene054179:intron 55.0%
CCATACTCCAGCATGCCAAG+GGG + chr6.1:28786363-28786382 MS.gene054179:intron 55.0%
CCCGTGAACCCGGAATTTCA+AGG + chr6.1:28786249-28786268 MS.gene054179:intron 55.0%
CCCTTCACAAATGGTGGCCA+CGG - chr6.1:28786505-28786524 None:intergenic 55.0%
CCGTGGCCACCATTTGTGAA+GGG + chr6.1:28786502-28786521 MS.gene054179:intron 55.0%
CTGATCCAGCCCTATGCTCA+TGG - chr6.1:28786548-28786567 None:intergenic 55.0%
GAAATTCCGGGTTCACGGGT+TGG - chr6.1:28786248-28786267 None:intergenic 55.0%
GAACGTTGCCTCCTCTTGCA+AGG - chr6.1:28787805-28787824 None:intergenic 55.0%
GACCATACTCCAGCATGCCA+AGG + chr6.1:28786361-28786380 MS.gene054179:intron 55.0%
GCAGGAGTGAGGGACATTCA+AGG + chr6.1:28787364-28787383 MS.gene054179:CDS 55.0%
GCCAAGCCAGCAAACTCTTC+AGG - chr6.1:28787400-28787419 None:intergenic 55.0%
TCTGCCTCACAATTCCACCG+TGG + chr6.1:28786485-28786504 MS.gene054179:intron 55.0%
TTATGAAGGGATGCCCGCCA+TGG - chr6.1:28786440-28786459 None:intergenic 55.0%
TTCACAAATGGTGGCCACGG+TGG - chr6.1:28786502-28786521 None:intergenic 55.0%
TTGACTTCCTTGGCCATGGC+GGG + chr6.1:28786424-28786443 MS.gene054179:intron 55.0%
!! CGGGAAAGTACCGGTGAAGT+TGG - chr6.1:28787560-28787579 None:intergenic 55.0%
!! GCGGTGTTGTCAATGGTGGA+AGG + chr6.1:28786151-28786170 MS.gene054179:intron 55.0%
ATGGACCCAACCCGTGAACC+CGG + chr6.1:28786239-28786258 MS.gene054179:intron 60.0%
CCCCTTGGCATGCTGGAGTA+TGG - chr6.1:28786366-28786385 None:intergenic 60.0%
GCAAGGGCAGTTGCTCCTAC+AGG + chr6.1:28785857-28785876 MS.gene054179:CDS 60.0%
GCGAAACTGGGATAGCGCCA+CGG - chr6.1:28786724-28786743 None:intergenic 60.0%
GTGGCCACGGTGGAATTGTG+AGG - chr6.1:28786492-28786511 None:intergenic 60.0%
AGGGATGCCCGCCATGGCCA+AGG - chr6.1:28786434-28786453 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr6.1 gene 28785479 28787904 28785479 ID=MS.gene054179
chr6.1 mRNA 28785479 28787904 28785479 ID=MS.gene054179.t1;Parent=MS.gene054179
chr6.1 exon 28785479 28785927 28785479 ID=MS.gene054179.t1.exon1;Parent=MS.gene054179.t1
chr6.1 CDS 28785479 28785927 28785479 ID=cds.MS.gene054179.t1;Parent=MS.gene054179.t1
chr6.1 exon 28787324 28787568 28787324 ID=MS.gene054179.t1.exon2;Parent=MS.gene054179.t1
chr6.1 CDS 28787324 28787568 28787324 ID=cds.MS.gene054179.t1;Parent=MS.gene054179.t1
chr6.1 exon 28787701 28787904 28787701 ID=MS.gene054179.t1.exon3;Parent=MS.gene054179.t1
chr6.1 CDS 28787701 28787904 28787701 ID=cds.MS.gene054179.t1;Parent=MS.gene054179.t1
Gene Sequence

>MS.gene054179

ATGTTGGCTATCGATTCTACAAGAAAGATATCATTTAATAGAACAATTAGTAACGGTGATTTGGCAATTGTTTATGAAAGGCATGATAGCATGAAGCCAGTAACAGTATCTGAGGGATCGGTTTTACAGAATCGATTCGGTGTTTTTAAACATTCAGATTGGATAGGAAAGTCATTTGGGTCTAAAGTATTCAACAATAAGGGCGGTTTTGTTTACTTATTAGCTCCTACTCCTGAATTATGGACTTTAGTTTTAAGCCACAGGACACAGATTCTATATATTGCAGATATCAGCTTTGTTGTTATGTATTTGGAGATTGTTCCTGGTTGTGTCGTTCTTGAATCTGGCACTGGAAGTGGGTCTTTAACTACTTCACTTGCAAGGGCAGTTGCTCCTACAGGACATGTTTATACGTTTGATTTTCACGAACAAAGATCTGGATCAGCTAGGTAATACTGCACTTGTCTTTCTCTGACGATATTTGATATACATGTGCTTATCTTTAGGCAATGTTGTATGTGTTATTTTCTAGTGTTGTCAACTATTGGCTCTATAGCATAGCGGATTTTGATGAATCCGCTAAAAAAAACTGCAACACTATCATAGTTTTGCCGTTATTCTAGTTTTTTGGTAGTTGATGTAGCAACAATAGCAGACAAAAGAGAATTTTAGGCGGTGTTGTCAATGGTGGAAGGCTAAAAATCTGCCATATAAACACACCATTACAGTCTGAAAATCGTATTTTTGGTGAAACGTGGTCATGGACCCAACCCGTGAACCCGGAATTTCAAGGAAGATAACCTGAATCAATGAATTGCAAGATTCCACCGTACCTACAATTTTCTGCGGTGATCCTGGCTGCACCCACCAAATTTTGATTCCGACCATACTCCAGCATGCCAAGGGGTATCAAAATCATACGATCCCATCAGTTTGATCGTGATTTTGACTTCCTTGGCCATGGCGGGCATCCCTTCATAAATTGGCTATGGCGGGCGTAAAAAAATCTGCCTCACAATTCCACCGTGGCCACCATTTGTGAAGGGCATGTTTATACATTTGATTTCCATGAGCATAGGGCTGGATCAGCTATGTAATATTGCATCAGCTAGGTGCTGCACCTGACTTCCTCTGATGTTTGATACATGTGTGTTTATCTTTAGCAAATGTCGAGAATGATTTTTTCAATAGCGGCACTATAGTGCTATCACATGGTGGATTTTGATGAGTCCACTATGAAAACCGTGGCGCTATCCCAGTTTCGCTATCAGGACAGTAGCGGCTGCTATTGAAGTTTTGCTGGCTGATATAGGAACTATATTGGGACTTTTTTTCATTTTTTTTAAAATAATAAAATTGAAGATCTGCATGTGTGCCCAACGTGGCTTATTTTTACAATGAAAAAACTTAGGAGTCAGTGAATATTACTCCTGTATTCACCGTAATACAATATATAAGTTACGTTCTGCTTCCATTCCCTTTTTATTTGCATGCAGGGTGCCTTGTTTTATATATAATTGACTTATTTCAACTGTTATGCGGTTTCTGCTATTGCCCGCAACACTATCTTCTATTTCACATAGCGGATTTTCTGTAATATGCTATTGATGGCACTTTCATGGCTTTGCATTATATTTTTTTCTCGTTCTAGTAATTGTAGGTAATATTTTATGTGATCTAATACTACTTTCAGAATGTCAGTGCTTAAGTGCAAATTTATTTAATTGTACTCAACTTTTGTTGGATTCATTTATGTGCTTGTATACACGTGTGTGCGGTGATAAACTTGAGATGTTCAATGCAAAGGTGAATTAGAAATATGAATACTTGTTTTTCTCCTTGTAGGGATGATTTTGAGAAGATTGGCATAAGCAGCTTAGTAACTGCAGGAGTGAGGGACATTCAAGGTGAGGGTTTTCCTGAAGAGTTTGCTGGCTTGGCTGATGCAGTTTTTTTGGATCTACCTCAACCTTGGCTAGCTATTCCTTCAGCTGCAAAAATGTTACGATATGACGGCACTTTATGTTCTTTCTCCCCGTGCATTGAACAAGTACAACGATCATGTGAAACAATGCAATCCAACTTCACCGGTACTTTCCCGATTTTCCTCTTTTATACGTATCTTATTTTTGCTCTCTCTCACACGTAGTAGTTATGCAACTAATGGTAATTTTATTCAAGCACTGACAAAACTCCTTTGTAAATGTTTGTTTATGTGAAAGATATAAGGACTTTTGAGGTACTCCTACGGACATACGAAGTTCGAGAAGGGAAAATGGAAAGCTCTGAGATTGACGGTAATGACGGCTCTAATGGTTCTCTTCCTTGCAAGAGGAGGCAACGTTCAGATGGAGCTAGCGTGGTGAGCTATTCTAATTCTTCTTCTGTTATGGCTAGACCATGTGGTGAAGCTAGAGGTCACACTG
Protein sequence

>MS.gene054179.t1

MLAIDSTRKISFNRTISNGDLAIVYERHDSMKPVTVSEGSVLQNRFGVFKHSDWIGKSFGSKVFNNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVVMYLEIVPGCVVLESGTGSGSLTTSLARAVAPTGHVYTFDFHEQRSGSARDDFEKIGISSLVTAGVRDIQGEGFPEEFAGLADAVFLDLPQPWLAIPSAAKMLRYDGTLCSFSPCIEQVQRSCETMQSNFTDIRTFEVLLRTYEVREGKMESSEIDGNDGSNGSLPCKRRQRSDGASVVSYSNSSSVMARPCGEARGHT