AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene05478

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene05478.t1 MTR_6g084700 80.049 812 66 8 1 716 1 812 0.0 1259
MS.gene05478.t1 MTR_1g050535 55.611 597 228 6 141 707 225 814 0.0 644
MS.gene05478.t1 MTR_1g050535 55.611 597 228 6 141 707 225 814 0.0 643
MS.gene05478.t1 MTR_8g464450 52.840 581 250 7 125 691 162 732 0.0 602
MS.gene05478.t1 MTR_3g055090 51.795 585 259 7 124 695 158 732 0.0 598
MS.gene05478.t1 MTR_3g113170 37.866 581 307 14 127 667 883 1449 8.49e-97 329
MS.gene05478.t1 MTR_3g113170 37.866 581 307 14 127 667 883 1449 1.08e-96 328
MS.gene05478.t1 MTR_1g023510 37.996 529 263 13 94 570 832 1347 7.54e-96 326
MS.gene05478.t1 MTR_3g064370 32.568 479 283 13 126 591 29 480 2.15e-55 197
MS.gene05478.t1 MTR_5g090300 34.979 466 261 15 126 576 29 467 3.33e-49 180
MS.gene05478.t1 MTR_3g449620 34.110 472 257 15 126 576 29 467 1.26e-47 176
MS.gene05478.t1 MTR_3g064370 30.952 420 260 12 185 591 1 403 7.21e-42 158
MS.gene05478.t1 MTR_3g064370 31.551 374 223 9 126 486 29 382 2.03e-38 147
MS.gene05478.t1 MTR_3g054420 38.462 195 115 4 514 707 88 278 2.55e-34 132
MS.gene05478.t1 MTR_2g039160 27.110 557 276 23 121 569 2 536 2.14e-33 135
MS.gene05478.t1 MTR_3g055020 37.306 193 112 5 504 695 245 429 5.62e-30 124
MS.gene05478.t1 MTR_3g033000 23.965 459 288 17 150 583 33 455 3.05e-23 104
MS.gene05478.t1 MTR_3g033000 24.311 399 243 16 150 523 33 397 6.16e-15 77.8
MS.gene05478.t1 MTR_1g087400 41.837 98 30 2 127 224 5 75 2.55e-14 68.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene05478.t1 AT1G62830 68.151 584 173 3 119 695 260 837 0.0 815
MS.gene05478.t1 AT3G10390 56.187 598 228 6 124 695 236 825 0.0 652
MS.gene05478.t1 AT3G10390 56.187 598 228 6 124 695 236 825 0.0 652
MS.gene05478.t1 AT3G10390 56.187 598 228 6 124 695 184 773 0.0 652
MS.gene05478.t1 AT3G10390 56.187 598 228 6 124 695 184 773 0.0 649
MS.gene05478.t1 AT3G13682 55.166 571 241 7 125 691 160 719 0.0 591
MS.gene05478.t1 AT4G16310 37.127 536 260 12 92 572 593 1106 1.11e-92 317
MS.gene05478.t1 AT4G16310 37.127 536 260 12 92 572 593 1106 1.11e-92 317
MS.gene05478.t1 AT4G16310 37.127 536 260 12 92 572 593 1106 1.11e-92 317
MS.gene05478.t1 AT4G16310 37.127 536 260 12 92 572 593 1106 1.11e-92 317
MS.gene05478.t1 AT4G16310 37.127 536 260 12 92 572 593 1106 1.11e-92 317
MS.gene05478.t1 AT1G65840 32.972 461 263 15 126 569 30 461 9.07e-56 199
MS.gene05478.t1 AT3G59050 34.125 463 263 16 126 573 30 465 1.48e-55 198
MS.gene05478.t1 AT2G43020 33.775 453 258 15 139 576 42 467 2.55e-54 194
MS.gene05478.t1 AT4G29720 27.037 540 292 20 123 572 3 530 1.03e-31 130
MS.gene05478.t1 AT5G13700 25.771 454 259 19 150 573 30 435 1.37e-25 111

Find 201 sgRNAs with CRISPR-Local

Find 252 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAATGTTGAATCGCGATTT+TGG 0.135598 6.2:-83507834 MS.gene05478:CDS
CGCTTTGTACTGTGCCTTTA+GGG 0.146452 6.2:-83508712 MS.gene05478:CDS
CGAATTTGTCTATGGCTTAT+TGG 0.158377 6.2:-83508919 MS.gene05478:CDS
TCTTGTGGCTGCGGCGGATT+TGG 0.188970 6.2:-83509290 MS.gene05478:CDS
GAATTGGCATCTTGCTAATT+TGG 0.191854 6.2:-83508966 MS.gene05478:CDS
GCGCTTTGTACTGTGCCTTT+AGG 0.194066 6.2:-83508713 MS.gene05478:CDS
TATGTTGCTGTTGGATCTTC+AGG 0.223351 6.2:-83508278 MS.gene05478:CDS
TCAATACTTAGGGTTAAAAT+TGG 0.230929 6.2:-83507957 MS.gene05478:CDS
AGATTGTTACGGTGTCGTTT+TGG 0.232996 6.2:+83509713 None:intergenic
GGTCAGTTTAGTTGGGAAAA+AGG 0.251761 6.2:-83507811 MS.gene05478:CDS
AAGAGTGTTGATGTTCCTTT+AGG 0.261370 6.2:-83509055 MS.gene05478:CDS
TGAAACCTGACCTGAATTTC+GGG 0.264220 6.2:-83508028 MS.gene05478:CDS
GTGAAACCTGACCTGAATTT+CGG 0.266040 6.2:-83508029 MS.gene05478:CDS
TTGGGTGGAAGTGTTCTTAC+TGG 0.270102 6.2:-83509271 MS.gene05478:CDS
AGGATTCGGGTTGCTGAATA+AGG 0.282210 6.2:-83508618 MS.gene05478:CDS
GGTGATCATTGTTTCATTCC+TGG 0.293333 6.2:-83508869 MS.gene05478:CDS
GAATTTGTCTATGGCTTATT+GGG 0.299575 6.2:-83508918 MS.gene05478:CDS
CTTGTGGCTGCGGCGGATTT+GGG 0.302997 6.2:-83509289 MS.gene05478:CDS
TGGTGCCGGGTTGGCGGGTT+TGG 0.308679 6.2:-83509434 MS.gene05478:CDS
TCTTATGTCGAATTTGTCTA+TGG 0.312805 6.2:-83508927 MS.gene05478:CDS
CAGAGAAGCTCCCGAAATTC+AGG 0.314383 6.2:+83508018 None:intergenic
TTGATTCTGAGGTTGATTCT+AGG 0.314623 6.2:-83509147 MS.gene05478:CDS
TCCGAAGGGGATTGTTGTTC+CGG 0.317671 6.2:-83508363 MS.gene05478:CDS
GACGGGAGGGTGTTCTTTGC+TGG 0.323842 6.2:-83508221 MS.gene05478:CDS
TTTGATGGTGATGGTGGTGG+TGG 0.324059 6.2:-83509319 MS.gene05478:CDS
CTTGTTGTTGCTGTTGTTGT+TGG 0.324895 6.2:+83509650 None:intergenic
AAGAGAGGATGTTGTTGAAT+TGG 0.333502 6.2:-83508982 MS.gene05478:CDS
TCGCCGGAGAAGCTGCTCTT+AGG 0.336308 6.2:-83508451 MS.gene05478:CDS
TTGAATCTAGTTCAATACTT+AGG 0.338543 6.2:-83507968 MS.gene05478:CDS
GGTGATGGTGGTGGTGGGGA+TGG 0.341179 6.2:-83509313 MS.gene05478:CDS
AGAGGGAGTGTGATTGTTAT+TGG 0.351894 6.2:-83509454 MS.gene05478:CDS
GTCTATGGCTTATTGGGATC+AGG 0.360792 6.2:-83508912 MS.gene05478:CDS
CAATTCATAGGTTAGGATTC+GGG 0.362726 6.2:-83508631 MS.gene05478:CDS
TTCTGACATTTGTGTATCAA+TGG 0.366809 6.2:+83509783 None:intergenic
AGTGTTCTTACTGGTGTTAA+TGG 0.367884 6.2:-83509262 MS.gene05478:CDS
TTTCGTGGTAGGGTAGGGTT+AGG 0.370277 6.2:+83509613 None:intergenic
CAGCTTAATGAAGCAGAAAA+TGG 0.371967 6.2:-83507735 MS.gene05478:CDS
GAACTAGATTCAAGATCATT+CGG 0.371980 6.2:+83507978 None:intergenic
TTCTTACTCTGAGGAAGATC+AGG 0.374652 6.2:+83508659 None:intergenic
TGTTGTTCCGGAGCCCGTTC+AGG 0.375878 6.2:-83508351 MS.gene05478:CDS
GATTCAAGATCATTCGGGTT+TGG 0.378278 6.2:+83507984 None:intergenic
GGCGTTTCGTAGGGTTTATA+AGG 0.379407 6.2:-83509020 MS.gene05478:CDS
ATTTGCCGCTTCATAAGGTT+AGG 0.384593 6.2:-83509207 MS.gene05478:CDS
AGCCACAAGAAGCGGGCCTC+CGG 0.384980 6.2:+83508478 None:intergenic
TGAATCTAGTTCAATACTTA+GGG 0.388262 6.2:-83507967 MS.gene05478:CDS
GCAATTCATAGGTTAGGATT+CGG 0.391500 6.2:-83508632 MS.gene05478:CDS
GTGTTCTTACTGGTGTTAAT+GGG 0.393336 6.2:-83509261 MS.gene05478:CDS
TTCTGAGTTTAGCTCAGTGT+TGG 0.395126 6.2:+83509810 None:intergenic
GTTATTGGTGCCGGGTTGGC+GGG 0.401105 6.2:-83509439 MS.gene05478:CDS
TCTTATTCTTATGTTGCTGT+TGG 0.401437 6.2:-83508287 MS.gene05478:CDS
CTTCTTGTGGCTCTTGTCGC+CGG 0.408698 6.2:-83508467 MS.gene05478:CDS
GGGGTGCTGGTGTGTACTGA+TGG 0.412671 6.2:-83508758 MS.gene05478:CDS
AACTAGATTCAAGATCATTC+GGG 0.414661 6.2:+83507979 None:intergenic
AGGGTTTAAAGTTTGTATTT+TGG 0.415674 6.2:-83509386 MS.gene05478:CDS
ATTAGATTTGCCGCTTCATA+AGG 0.416276 6.2:-83509212 MS.gene05478:CDS
GATATACTTTCAGAGAGTAT+TGG 0.417231 6.2:-83508245 MS.gene05478:CDS
TGAGGTTGATTCTAGGGTTG+AGG 0.427964 6.2:-83509140 MS.gene05478:CDS
AAGGATGCAATTCATAGGTT+AGG 0.428067 6.2:-83508638 MS.gene05478:CDS
GGTAGGGTAGGGTTAGGGTT+TGG 0.428542 6.2:+83509619 None:intergenic
CGCCGTTGGAGTCTGTCAAA+AGG 0.429103 6.2:-83508415 MS.gene05478:CDS
ACCCTACTGAAATTGCAATC+AGG 0.429506 6.2:+83509491 None:intergenic
TTGATGGTGATGGTGGTGGT+GGG 0.429726 6.2:-83509318 MS.gene05478:CDS
TGGGGATGGTCTTGTGGCTG+CGG 0.432164 6.2:-83509299 MS.gene05478:CDS
TTGTTGTTGCTGTTGTTGTT+GGG 0.433511 6.2:+83509651 None:intergenic
ATGTTTGATGGTGATGGTGG+TGG 0.433708 6.2:-83509322 MS.gene05478:CDS
TGTTATTGGTGCCGGGTTGG+CGG 0.438476 6.2:-83509440 MS.gene05478:CDS
TATAGCTATGCTTCTGTGTC+CGG 0.439518 6.2:-83508497 MS.gene05478:CDS
AGAGTGTTGATGTTCCTTTA+GGG 0.447197 6.2:-83509054 MS.gene05478:CDS
GTAGGGATCGACCGGGTGGG+AGG 0.448094 6.2:-83509360 MS.gene05478:CDS
TCTGAGTTTAGCTCAGTGTT+GGG 0.452317 6.2:+83509811 None:intergenic
TGATGGTGATGGTGGTGGTG+GGG 0.454103 6.2:-83509317 MS.gene05478:CDS
AATGCTCCATGCATTGTTGC+AGG 0.458661 6.2:+83508176 None:intergenic
GTTTGTTCGCGTGTTGGCTG+AGG 0.458763 6.2:-83508834 MS.gene05478:CDS
AAGACGAAGAGGATGTTTGA+TGG 0.462613 6.2:-83509334 MS.gene05478:CDS
TGTATTAAGTACGGAAGTGA+TGG 0.463104 6.2:-83508779 MS.gene05478:CDS
CGAGTACAGACTGCCTGAAC+GGG 0.463252 6.2:+83508338 None:intergenic
GGGTTTCATGTAATTGAGAG+AGG 0.466677 6.2:-83509472 MS.gene05478:CDS
TGACCGAGGATTTGAGTATG+AGG 0.466769 6.2:-83508538 MS.gene05478:CDS
TGCATTGCTTTCGAAGAAGC+AGG 0.471890 6.2:-83507898 MS.gene05478:CDS
GCAGAAAATGGTCTTGTCCA+TGG 0.472749 6.2:-83507723 MS.gene05478:CDS
ATACTTAGGGTTAAAATTGG+AGG 0.474385 6.2:-83507954 MS.gene05478:CDS
TGGTGGTGGGGATGGTCTTG+TGG 0.474912 6.2:-83509305 MS.gene05478:CDS
ACAGTCCTCCCATAGAAAAT+CGG 0.475535 6.2:+83508806 None:intergenic
TGATTCTGAGGTTGATTCTA+GGG 0.476292 6.2:-83509146 MS.gene05478:CDS
TCTGACATTTGTGTATCAAT+GGG 0.477512 6.2:+83509784 None:intergenic
AAACCTAAGAGCAGCTTCTC+CGG 0.481395 6.2:+83508448 None:intergenic
TCCTTTAGGGACAGCGTTGG+AGG 0.482675 6.2:-83509041 MS.gene05478:CDS
TTTGGAAGGTAGGGATCGAC+CGG 0.483867 6.2:-83509368 MS.gene05478:CDS
GTCCGGAGGCCCGCTTCTTG+TGG 0.487113 6.2:-83508480 MS.gene05478:CDS
TGTTTGTCCTCTTTATATGC+CGG 0.487898 6.2:-83509182 MS.gene05478:CDS
GTAAGAAGGATGCAATTCAT+AGG 0.491494 6.2:-83508643 MS.gene05478:CDS
GGTGGGAATATCGATACGTT+TGG 0.492909 6.2:-83508566 MS.gene05478:CDS
TCTTTCTTAAGAACCCCTAA+AGG 0.493042 6.2:+83508698 None:intergenic
GTGTGATTGTTATTGGTGCC+GGG 0.497179 6.2:-83509447 MS.gene05478:CDS
AGATTGAAGGAGACGAAAAT+CGG 0.497202 6.2:-83507860 MS.gene05478:CDS
TAGAGAGGGTTTGTAAGCTT+AGG 0.499527 6.2:-83509099 MS.gene05478:CDS
TGATCACCACCCATCTCATA+AGG 0.500642 6.2:+83508884 None:intergenic
GCAGCAGTTTCGTGCGGACA+TGG 0.500964 6.2:-83508735 MS.gene05478:CDS
TTCGTGGTAGGGTAGGGTTA+GGG 0.503684 6.2:+83509614 None:intergenic
CAGCGTTGGAGGCGTTTCGT+AGG 0.505275 6.2:-83509030 MS.gene05478:CDS
TTTGCCGCTTCATAAGGTTA+GGG 0.505729 6.2:-83509206 MS.gene05478:CDS
GCCTCCAACGCTGTCCCTAA+AGG 0.508131 6.2:+83509040 None:intergenic
CAGACTGCCTGAACGGGCTC+CGG 0.509810 6.2:+83508344 None:intergenic
CGACAAGAGCCACAAGAAGC+GGG 0.513712 6.2:+83508471 None:intergenic
TTTCCATCCGGCATATAAAG+AGG 0.514177 6.2:+83509175 None:intergenic
ATTGAGTTGAGTCAGATTGA+AGG 0.514975 6.2:-83507873 MS.gene05478:CDS
CAATGCATAAAGATAAAGAC+TGG 0.515438 6.2:+83507914 None:intergenic
TTCAGGCAGTCTGTACTCGA+TGG 0.522232 6.2:-83508334 MS.gene05478:CDS
TGGGAAGGATAGTTGTATCA+TGG 0.522562 6.2:-83507703 MS.gene05478:CDS
TTGTTGTTGGGAGGATGAAT+CGG 0.522762 6.2:+83509663 None:intergenic
CAGAAAATGGTCTTGTCCAT+GGG 0.522807 6.2:-83507722 MS.gene05478:CDS
CCCTACTGAAATTGCAATCA+GGG 0.522982 6.2:+83509492 None:intergenic
TCCGGAACAACAATCCCCTT+CGG 0.525102 6.2:+83508362 None:intergenic
GTTTGAGATGATGTCGCCGT+TGG 0.525621 6.2:-83508429 MS.gene05478:CDS
AAGCTCCCGAAATTCAGGTC+AGG 0.529964 6.2:+83508023 None:intergenic
TCAGGATGATCCTTATGAGA+TGG 0.530816 6.2:-83508894 MS.gene05478:CDS
GGCAACCAAACCCGCCAACC+CGG 0.531009 6.2:+83509429 None:intergenic
GGATGGTCTTGTGGCTGCGG+CGG 0.531611 6.2:-83509296 MS.gene05478:CDS
TCAGGCAGTCTGTACTCGAT+GGG 0.534093 6.2:-83508333 MS.gene05478:CDS
TTTGTTCGCGTGTTGGCTGA+GGG 0.535278 6.2:-83508833 MS.gene05478:CDS
TGTGACGGTGGTTCCGATGA+GGG 0.537164 6.2:+83509760 None:intergenic
GTACGGAAGTGATGGGGTGC+TGG 0.539774 6.2:-83508771 MS.gene05478:CDS
TTTGGAAAGGGAAGTTGTTG+CGG 0.540117 6.2:+83509555 None:intergenic
AGTGTGATTGTTATTGGTGC+CGG 0.540389 6.2:-83509448 MS.gene05478:CDS
GGTTTCATGTAATTGAGAGA+GGG 0.541876 6.2:-83509471 MS.gene05478:CDS
CCCTGATTGCAATTTCAGTA+GGG 0.543752 6.2:-83509492 MS.gene05478:CDS
GTATTAAGTACGGAAGTGAT+GGG 0.544748 6.2:-83508778 MS.gene05478:CDS
GCCGTTGGAGTCTGTCAAAA+GGG 0.545041 6.2:-83508414 MS.gene05478:CDS
AGCGTTGGAGGCGTTTCGTA+GGG 0.545418 6.2:-83509029 MS.gene05478:CDS
TGTTTAATAAGTTGTTAGAG+AGG 0.546125 6.2:-83509114 MS.gene05478:CDS
CAGGATGATCCTTATGAGAT+GGG 0.547234 6.2:-83508893 MS.gene05478:CDS
ACTGTGGAGTGTATTAAGTA+CGG 0.549350 6.2:-83508788 MS.gene05478:CDS
GCGACAAGAGCCACAAGAAG+CGG 0.549634 6.2:+83508470 None:intergenic
GTGGCTGCGGCGGATTTGGG+TGG 0.550218 6.2:-83509286 MS.gene05478:CDS
GCCCTGATTGCAATTTCAGT+AGG 0.550601 6.2:-83509493 MS.gene05478:CDS
GATTGTTATTGGTGCCGGGT+TGG 0.550853 6.2:-83509443 MS.gene05478:CDS
TAGGGATCGACCGGGTGGGA+GGG 0.552307 6.2:-83509359 MS.gene05478:CDS
CAGTACCCTGCAACAATGCA+TGG 0.555950 6.2:-83508182 MS.gene05478:CDS
TTTCAGAGAGTATTGGAGAC+GGG 0.556898 6.2:-83508238 MS.gene05478:CDS
AGTGTTGGGAGGTGAGTGCA+TGG 0.557785 6.2:+83509825 None:intergenic
GTTTAATAAGTTGTTAGAGA+GGG 0.558201 6.2:-83509113 MS.gene05478:CDS
TGTCCTCTTTATATGCCGGA+TGG 0.559990 6.2:-83509178 MS.gene05478:CDS
TGGGGTTGTGGTGATTCCGA+CGG 0.561467 6.2:+83509733 None:intergenic
GGGTGCTGGTGTGTACTGAT+GGG 0.562552 6.2:-83508757 MS.gene05478:CDS
TGATCTTCCTCAGAGTAAGA+AGG 0.563913 6.2:-83508657 MS.gene05478:CDS
GACCGAGGATTTGAGTATGA+GGG 0.565102 6.2:-83508537 MS.gene05478:CDS
ATCATTGTTTCATTCCTGGA+GGG 0.570638 6.2:-83508865 MS.gene05478:CDS
AGGTCTCAGCAACTTTATGT+TGG 0.572014 6.2:+83507758 None:intergenic
AGGATGTTTGATGGTGATGG+TGG 0.572599 6.2:-83509325 MS.gene05478:CDS
CAGAGAGTATTGGAGACGGG+AGG 0.575157 6.2:-83508235 MS.gene05478:CDS
GAGTTTAGCTCAGTGTTGGG+AGG 0.575811 6.2:+83509814 None:intergenic
TTCGTCTTAACCCTCCCACC+CGG 0.576628 6.2:+83509349 None:intergenic
TTGCTGAGGATAAAGAAGAG+AGG 0.577715 6.2:-83508997 MS.gene05478:CDS
TCGAGTACAGACTGCCTGAA+CGG 0.577795 6.2:+83508337 None:intergenic
ACCGAGGATTTGAGTATGAG+GGG 0.581726 6.2:-83508536 MS.gene05478:CDS
TTGTTGCTGTTGTTGTTGGG+AGG 0.586183 6.2:+83509654 None:intergenic
AAGAGGATGTTTGATGGTGA+TGG 0.586715 6.2:-83509328 MS.gene05478:CDS
TGAGACGTTTGTTCGCGTGT+TGG 0.592879 6.2:-83508840 MS.gene05478:CDS
TCCCCTCATACTCAAATCCT+CGG 0.593722 6.2:+83508535 None:intergenic
GGTTGTGGTGATTCCGACGG+TGG 0.594206 6.2:+83509736 None:intergenic
GAGGGTGTTCTTTGCTGGAG+AGG 0.596154 6.2:-83508216 MS.gene05478:CDS
GATTCCGACGGTGGTTGTGA+CGG 0.597156 6.2:+83509745 None:intergenic
TGTTCCTTTAGGGACAGCGT+TGG 0.602218 6.2:-83509044 MS.gene05478:CDS
AAATGGTCTTGTCCATGGGA+AGG 0.604796 6.2:-83507718 MS.gene05478:CDS
GGAGTCTGTCAAAAGGGTGT+TGG 0.607885 6.2:-83508408 MS.gene05478:CDS
GAAGGTAGGGATCGACCGGG+TGG 0.607969 6.2:-83509364 MS.gene05478:CDS
CTTTCAGAGAGTATTGGAGA+CGG 0.608296 6.2:-83508239 MS.gene05478:CDS
ACAAATGTCAGAACCCTCAT+CGG 0.610378 6.2:-83509773 MS.gene05478:CDS
ACAAACGTCTCATTCCCTCC+AGG 0.610632 6.2:+83508851 None:intergenic
GGTGTTAATGGGAATCCGCT+TGG 0.610906 6.2:-83509250 MS.gene05478:CDS
AAGGTAGGGATCGACCGGGT+GGG 0.612210 6.2:-83509363 MS.gene05478:CDS
TGATACAACTATCCTTCCCA+TGG 0.613592 6.2:+83507706 None:intergenic
TAGGGTTTATAAGGTTGCTG+AGG 0.615237 6.2:-83509011 MS.gene05478:CDS
AGAGAGTATTGGAGACGGGA+GGG 0.615836 6.2:-83508234 MS.gene05478:CDS
TGGAAAATGTGTTGATTCTG+AGG 0.616208 6.2:-83509158 MS.gene05478:CDS
TTGTGACGGTGGTTCCGATG+AGG 0.617066 6.2:+83509759 None:intergenic
TTGTTGGGAGGATGAATCGG+AGG 0.619106 6.2:+83509666 None:intergenic
ACATCCCTAACCTTATGAAG+CGG 0.624599 6.2:+83509202 None:intergenic
GATCATTGTTTCATTCCTGG+AGG 0.626617 6.2:-83508866 MS.gene05478:CDS
TAGGCAAGCTATGATTGATG+AGG 0.627755 6.2:-83509080 MS.gene05478:CDS
GCTTTGTACTGTGCCTTTAG+GGG 0.628229 6.2:-83508711 MS.gene05478:CDS
GGAAAAGATGAATTCGCGTA+CGG 0.634759 6.2:-83508311 MS.gene05478:CDS
TCCGACGGTGGTTGTGACGG+TGG 0.635828 6.2:+83509748 None:intergenic
TCTTACTCTGAGGAAGATCA+GGG 0.636544 6.2:+83508660 None:intergenic
AGCTATGCTTCTGTGTCCGG+AGG 0.638434 6.2:-83508494 MS.gene05478:CDS
AATGCATAAAGATAAAGACT+GGG 0.646834 6.2:+83507915 None:intergenic
ACCACCGTCACAACCACCGT+CGG 0.649079 6.2:-83509749 MS.gene05478:CDS
ATGCATGGAGCATTCATGAG+TGG 0.656805 6.2:-83508167 MS.gene05478:CDS
TACGTTTGGTCATTTGACCG+AGG 0.661211 6.2:-83508552 MS.gene05478:CDS
ATGCATAAAGATAAAGACTG+GGG 0.664278 6.2:+83507916 None:intergenic
ATTGCATCCTTCTTACTCTG+AGG 0.666273 6.2:+83508650 None:intergenic
CAGGCAGTCTGTACTCGATG+GGG 0.668068 6.2:-83508332 MS.gene05478:CDS
ATGCTCCATGCATTGTTGCA+GGG 0.670835 6.2:+83508177 None:intergenic
TACCACGAAAACGACGTCGT+CGG 0.672979 6.2:-83509600 MS.gene05478:CDS
TGCATAAAGATAAAGACTGG+GGG 0.678482 6.2:+83507917 None:intergenic
GTGGGAGGGTTAAGACGAAG+AGG 0.679245 6.2:-83509345 MS.gene05478:CDS
TATTAAGTACGGAAGTGATG+GGG 0.686971 6.2:-83508777 MS.gene05478:CDS
TGATGGGCAGCAGTTTCGTG+CGG 0.691444 6.2:-83508741 MS.gene05478:CDS
TGTCTAGCAAGAACACCAAG+CGG 0.711945 6.2:+83509235 None:intergenic
TTGGAAGGTAGGGATCGACC+GGG 0.714334 6.2:-83509367 MS.gene05478:CDS
GATGATCCTTATGAGATGGG+TGG 0.750776 6.2:-83508890 MS.gene05478:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ACATTTTAAGAGTTACAAAA+CGG - chr6.2:83509391-83509410 MS.gene05478:CDS 20.0%
!!! TTTAAAGTTTGTATTTTGGA+AGG - chr6.2:83508136-83508155 MS.gene05478:CDS 20.0%
! AATGCATAAAGATAAAGACT+GGG + chr6.2:83509606-83509625 None:intergenic 25.0%
! GTTTAATAAGTTGTTAGAGA+GGG - chr6.2:83508405-83508424 MS.gene05478:CDS 25.0%
! TCAATACTTAGGGTTAAAAT+TGG - chr6.2:83509561-83509580 MS.gene05478:CDS 25.0%
! TGTTTAATAAGTTGTTAGAG+AGG - chr6.2:83508404-83508423 MS.gene05478:CDS 25.0%
!! TGAATCTAGTTCAATACTTA+GGG - chr6.2:83509551-83509570 MS.gene05478:CDS 25.0%
!! TTGAATCTAGTTCAATACTT+AGG - chr6.2:83509550-83509569 MS.gene05478:CDS 25.0%
!!! AGGGTTTAAAGTTTGTATTT+TGG - chr6.2:83508132-83508151 MS.gene05478:CDS 25.0%
!!! ATGTTTTTTGTTGTGTTAGA+TGG + chr6.2:83509435-83509454 None:intergenic 25.0%
AACTAGATTCAAGATCATTC+GGG + chr6.2:83509542-83509561 None:intergenic 30.0%
ATACTTAGGGTTAAAATTGG+AGG - chr6.2:83509564-83509583 MS.gene05478:CDS 30.0%
ATGCATAAAGATAAAGACTG+GGG + chr6.2:83509605-83509624 None:intergenic 30.0%
CAATGCATAAAGATAAAGAC+TGG + chr6.2:83509607-83509626 None:intergenic 30.0%
GAACTAGATTCAAGATCATT+CGG + chr6.2:83509543-83509562 None:intergenic 30.0%
GAATTTGTCTATGGCTTATT+GGG - chr6.2:83508600-83508619 MS.gene05478:CDS 30.0%
TCTGACATTTGTGTATCAAT+GGG + chr6.2:83507737-83507756 None:intergenic 30.0%
TCTTATGTCGAATTTGTCTA+TGG - chr6.2:83508591-83508610 MS.gene05478:CDS 30.0%
TCTTATTCTTATGTTGCTGT+TGG - chr6.2:83509231-83509250 MS.gene05478:CDS 30.0%
TTCTGACATTTGTGTATCAA+TGG + chr6.2:83507738-83507757 None:intergenic 30.0%
! AAAATGTTGAATCGCGATTT+TGG - chr6.2:83509684-83509703 MS.gene05478:CDS 30.0%
! AAATGTTGAATCGCGATTTT+GGG - chr6.2:83509685-83509704 MS.gene05478:CDS 30.0%
! AAGGATATTTTTCATCCGAA+GGG - chr6.2:83509141-83509160 MS.gene05478:CDS 30.0%
! GATATACTTTCAGAGAGTAT+TGG - chr6.2:83509273-83509292 MS.gene05478:CDS 30.0%
!! AAGTTTGTATTTTGGAAGGT+AGG - chr6.2:83508140-83508159 MS.gene05478:CDS 30.0%
!! AGTTTGTATTTTGGAAGGTA+GGG - chr6.2:83508141-83508160 MS.gene05478:CDS 30.0%
AAGAGAGGATGTTGTTGAAT+TGG - chr6.2:83508536-83508555 MS.gene05478:CDS 35.0%
AAGGATGCAATTCATAGGTT+AGG - chr6.2:83508880-83508899 MS.gene05478:CDS 35.0%
ACTGTGGAGTGTATTAAGTA+CGG - chr6.2:83508730-83508749 MS.gene05478:CDS 35.0%
AGATTGAAGGAGACGAAAAT+CGG - chr6.2:83509658-83509677 MS.gene05478:CDS 35.0%
ATCATTGTTTCATTCCTGGA+GGG - chr6.2:83508653-83508672 MS.gene05478:CDS 35.0%
CAATTCATAGGTTAGGATTC+GGG - chr6.2:83508887-83508906 MS.gene05478:CDS 35.0%
CACAACAAAAAACATCGATG+AGG - chr6.2:83509440-83509459 MS.gene05478:CDS 35.0%
CAGCTTAATGAAGCAGAAAA+TGG - chr6.2:83509783-83509802 MS.gene05478:CDS 35.0%
CGAATTTGTCTATGGCTTAT+TGG - chr6.2:83508599-83508618 MS.gene05478:CDS 35.0%
GAATTGGCATCTTGCTAATT+TGG - chr6.2:83508552-83508571 MS.gene05478:CDS 35.0%
GCAATTCATAGGTTAGGATT+CGG - chr6.2:83508886-83508905 MS.gene05478:CDS 35.0%
GGTTTCATGTAATTGAGAGA+GGG - chr6.2:83508047-83508066 MS.gene05478:CDS 35.0%
GTAAGAAGGATGCAATTCAT+AGG - chr6.2:83508875-83508894 MS.gene05478:CDS 35.0%
GTATTAAGTACGGAAGTGAT+GGG - chr6.2:83508740-83508759 MS.gene05478:CDS 35.0%
GTCAGTTTAGTTGGGAAAAA+GGG - chr6.2:83509708-83509727 MS.gene05478:CDS 35.0%
TAAAAACACTAATTGTCGCG+CGG + chr6.2:83508113-83508132 None:intergenic 35.0%
TATTAAGTACGGAAGTGATG+GGG - chr6.2:83508741-83508760 MS.gene05478:CDS 35.0%
TCTTTCTTAAGAACCCCTAA+AGG + chr6.2:83508823-83508842 None:intergenic 35.0%
TGCATAAAGATAAAGACTGG+GGG + chr6.2:83509604-83509623 None:intergenic 35.0%
TGTATTAAGTACGGAAGTGA+TGG - chr6.2:83508739-83508758 MS.gene05478:CDS 35.0%
TGTTTGTCCTCTTTATATGC+CGG - chr6.2:83508336-83508355 MS.gene05478:CDS 35.0%
! AATGTTGAATCGCGATTTTG+GGG - chr6.2:83509686-83509705 MS.gene05478:CDS 35.0%
! AGGATATTTTTCATCCGAAG+GGG - chr6.2:83509142-83509161 MS.gene05478:CDS 35.0%
! AGTGTTCTTACTGGTGTTAA+TGG - chr6.2:83508256-83508275 MS.gene05478:CDS 35.0%
! ATTAGATTTGCCGCTTCATA+AGG - chr6.2:83508306-83508325 MS.gene05478:CDS 35.0%
! CTGTTTCCGACTAATTTTTG+GGG - chr6.2:83508931-83508950 MS.gene05478:CDS 35.0%
! GAAGGATATTTTTCATCCGA+AGG - chr6.2:83509140-83509159 MS.gene05478:CDS 35.0%
! GAATCAACACATTTTCCATC+CGG + chr6.2:83508358-83508377 None:intergenic 35.0%
! GTGTTCTTACTGGTGTTAAT+GGG - chr6.2:83508257-83508276 MS.gene05478:CDS 35.0%
! TTGTTGTTGCTGTTGTTGTT+GGG + chr6.2:83507870-83507889 None:intergenic 35.0%
! TTTTCTGCTTCATTAAGCTG+AGG + chr6.2:83509783-83509802 None:intergenic 35.0%
!! AAGAGTGTTGATGTTCCTTT+AGG - chr6.2:83508463-83508482 MS.gene05478:CDS 35.0%
!! AGAGTGTTGATGTTCCTTTA+GGG - chr6.2:83508464-83508483 MS.gene05478:CDS 35.0%
!! ATTGAGTTGAGTCAGATTGA+AGG - chr6.2:83509645-83509664 MS.gene05478:CDS 35.0%
!! CGCGACAATTAGTGTTTTTA+GGG - chr6.2:83508113-83508132 MS.gene05478:CDS 35.0%
!! GCTGTTTCCGACTAATTTTT+GGG - chr6.2:83508930-83508949 MS.gene05478:CDS 35.0%
!! TGATTCTGAGGTTGATTCTA+GGG - chr6.2:83508372-83508391 MS.gene05478:CDS 35.0%
!! TGGAAAATGTGTTGATTCTG+AGG - chr6.2:83508360-83508379 MS.gene05478:CDS 35.0%
!! TTGATTCTGAGGTTGATTCT+AGG - chr6.2:83508371-83508390 MS.gene05478:CDS 35.0%
!!! AATTTTGAGGTGGGTTTTCA+GGG + chr6.2:83507832-83507851 None:intergenic 35.0%
!!! ATTTTGGGGTCAGTTTAGTT+GGG - chr6.2:83509700-83509719 MS.gene05478:CDS 35.0%
!!! TGCTGTTTCCGACTAATTTT+TGG - chr6.2:83508929-83508948 MS.gene05478:CDS 35.0%
AAGACGAAGAGGATGTTTGA+TGG - chr6.2:83508184-83508203 MS.gene05478:CDS 40.0%
AAGATAAAGACTGGGGGAAT+TGG + chr6.2:83509598-83509617 None:intergenic 40.0%
ACAAATGTCAGAACCCTCAT+CGG - chr6.2:83507745-83507764 MS.gene05478:CDS 40.0%
ACAGTCCTCCCATAGAAAAT+CGG + chr6.2:83508715-83508734 None:intergenic 40.0%
ACATCCCTAACCTTATGAAG+CGG + chr6.2:83508319-83508338 None:intergenic 40.0%
ACCCTACTGAAATTGCAATC+AGG + chr6.2:83508030-83508049 None:intergenic 40.0%
AGGTCTCAGCAACTTTATGT+TGG + chr6.2:83509763-83509782 None:intergenic 40.0%
ATTGCATCCTTCTTACTCTG+AGG + chr6.2:83508871-83508890 None:intergenic 40.0%
CAGGATGATCCTTATGAGAT+GGG - chr6.2:83508625-83508644 MS.gene05478:CDS 40.0%
CCCTACTGAAATTGCAATCA+GGG + chr6.2:83508029-83508048 None:intergenic 40.0%
CCCTGATTGCAATTTCAGTA+GGG - chr6.2:83508026-83508045 MS.gene05478:CDS 40.0%
GATCATTGTTTCATTCCTGG+AGG - chr6.2:83508652-83508671 MS.gene05478:CDS 40.0%
GGAAAAGATGAATTCGCGTA+CGG - chr6.2:83509207-83509226 MS.gene05478:CDS 40.0%
GGGTTTCATGTAATTGAGAG+AGG - chr6.2:83508046-83508065 MS.gene05478:CDS 40.0%
GGTCAGTTTAGTTGGGAAAA+AGG - chr6.2:83509707-83509726 MS.gene05478:CDS 40.0%
GGTGATCATTGTTTCATTCC+TGG - chr6.2:83508649-83508668 MS.gene05478:CDS 40.0%
GTGAAACCTGACCTGAATTT+CGG - chr6.2:83509489-83509508 MS.gene05478:CDS 40.0%
TAGAGAGGGTTTGTAAGCTT+AGG - chr6.2:83508419-83508438 MS.gene05478:CDS 40.0%
TAGGCAAGCTATGATTGATG+AGG - chr6.2:83508438-83508457 MS.gene05478:CDS 40.0%
TAGGGTTTATAAGGTTGCTG+AGG - chr6.2:83508507-83508526 MS.gene05478:CDS 40.0%
TATAGCTATGCTTCTGTGTC+CGG - chr6.2:83509021-83509040 MS.gene05478:CDS 40.0%
TCAGGATGATCCTTATGAGA+TGG - chr6.2:83508624-83508643 MS.gene05478:CDS 40.0%
TGAAACCTGACCTGAATTTC+GGG - chr6.2:83509490-83509509 MS.gene05478:CDS 40.0%
TGATACAACTATCCTTCCCA+TGG + chr6.2:83509815-83509834 None:intergenic 40.0%
TGATCTTCCTCAGAGTAAGA+AGG - chr6.2:83508861-83508880 MS.gene05478:CDS 40.0%
TGGGAAGGATAGTTGTATCA+TGG - chr6.2:83509815-83509834 MS.gene05478:CDS 40.0%
TTCCCACCCCAAAAATTAGT+CGG + chr6.2:83508940-83508959 None:intergenic 40.0%
TTGCTGAGGATAAAGAAGAG+AGG - chr6.2:83508521-83508540 MS.gene05478:CDS 40.0%
TTTCCATCCGGCATATAAAG+AGG + chr6.2:83508346-83508365 None:intergenic 40.0%
TTTGGAAAGGGAAGTTGTTG+CGG + chr6.2:83507966-83507985 None:intergenic 40.0%
! AGAGGGAGTGTGATTGTTAT+TGG - chr6.2:83508064-83508083 MS.gene05478:CDS 40.0%
! AGGGTCTTCCGATTTTCTAT+GGG - chr6.2:83508704-83508723 MS.gene05478:CDS 40.0%
! AGTGTGATTGTTATTGGTGC+CGG - chr6.2:83508070-83508089 MS.gene05478:CDS 40.0%
! ATTTGCCGCTTCATAAGGTT+AGG - chr6.2:83508311-83508330 MS.gene05478:CDS 40.0%
! GATGAATCGGAGGAATTTTG+AGG + chr6.2:83507845-83507864 None:intergenic 40.0%
! GATTCAAGATCATTCGGGTT+TGG + chr6.2:83509537-83509556 None:intergenic 40.0%
! GCGCGACAATTAGTGTTTTT+AGG - chr6.2:83508112-83508131 MS.gene05478:CDS 40.0%
! TATGTTGCTGTTGGATCTTC+AGG - chr6.2:83509240-83509259 MS.gene05478:CDS 40.0%
! TCTGAGTTTAGCTCAGTGTT+GGG + chr6.2:83507710-83507729 None:intergenic 40.0%
! TCTTACTCTGAGGAAGATCA+GGG + chr6.2:83508861-83508880 None:intergenic 40.0%
! TTCTGAGTTTAGCTCAGTGT+TGG + chr6.2:83507711-83507730 None:intergenic 40.0%
! TTCTTACTCTGAGGAAGATC+AGG + chr6.2:83508862-83508881 None:intergenic 40.0%
! TTGTTGTTGGGAGGATGAAT+CGG + chr6.2:83507858-83507877 None:intergenic 40.0%
! TTTGCCGCTTCATAAGGTTA+GGG - chr6.2:83508312-83508331 MS.gene05478:CDS 40.0%
!! AAGAGGATGTTTGATGGTGA+TGG - chr6.2:83508190-83508209 MS.gene05478:CDS 40.0%
!! CAGAAAATGGTCTTGTCCAT+GGG - chr6.2:83509796-83509815 MS.gene05478:CDS 40.0%
!! CTTGTTGTTGCTGTTGTTGT+TGG + chr6.2:83507871-83507890 None:intergenic 40.0%
!! CTTTCAGAGAGTATTGGAGA+CGG - chr6.2:83509279-83509298 MS.gene05478:CDS 40.0%
!! GTTTTCAGGGGAGATTGTTA+CGG + chr6.2:83507819-83507838 None:intergenic 40.0%
!! TTTCAGAGAGTATTGGAGAC+GGG - chr6.2:83509280-83509299 MS.gene05478:CDS 40.0%
!! TTTCCGACTAATTTTTGGGG+TGG - chr6.2:83508934-83508953 MS.gene05478:CDS 40.0%
!!! AAGGGTGTTGGATGTTTTGA+AGG - chr6.2:83509122-83509141 MS.gene05478:CDS 40.0%
!!! AATCGGAGGAATTTTGAGGT+GGG + chr6.2:83507841-83507860 None:intergenic 40.0%
!!! AGATTGTTACGGTGTCGTTT+TGG + chr6.2:83507808-83507827 None:intergenic 40.0%
!!! ATTGTTACGGTGTCGTTTTG+GGG + chr6.2:83507806-83507825 None:intergenic 40.0%
!!! ATTTTGAGGTGGGTTTTCAG+GGG + chr6.2:83507831-83507850 None:intergenic 40.0%
!!! GAATTTTGAGGTGGGTTTTC+AGG + chr6.2:83507833-83507852 None:intergenic 40.0%
!!! GATTGTTACGGTGTCGTTTT+GGG + chr6.2:83507807-83507826 None:intergenic 40.0%
!!! GATTTTGGGGTCAGTTTAGT+TGG - chr6.2:83509699-83509718 MS.gene05478:CDS 40.0%
!!! GTTTTTGCGGGTTTTGGAAA+GGG + chr6.2:83507978-83507997 None:intergenic 40.0%
!!! TTCCGACTAATTTTTGGGGT+GGG - chr6.2:83508935-83508954 MS.gene05478:CDS 40.0%
AAACCTAAGAGCAGCTTCTC+CGG + chr6.2:83509073-83509092 None:intergenic 45.0%
AATGCTCCATGCATTGTTGC+AGG + chr6.2:83509345-83509364 None:intergenic 45.0%
ACCGAGGATTTGAGTATGAG+GGG - chr6.2:83508982-83509001 MS.gene05478:CDS 45.0%
ATGCTCCATGCATTGTTGCA+GGG + chr6.2:83509344-83509363 None:intergenic 45.0%
CGCTTTGTACTGTGCCTTTA+GGG - chr6.2:83508806-83508825 MS.gene05478:CDS 45.0%
GACCGAGGATTTGAGTATGA+GGG - chr6.2:83508981-83509000 MS.gene05478:CDS 45.0%
GATGATCCTTATGAGATGGG+TGG - chr6.2:83508628-83508647 MS.gene05478:CDS 45.0%
GCCCTGATTGCAATTTCAGT+AGG - chr6.2:83508025-83508044 MS.gene05478:CDS 45.0%
GCTTTGTACTGTGCCTTTAG+GGG - chr6.2:83508807-83508826 MS.gene05478:CDS 45.0%
GGCGTTTCGTAGGGTTTATA+AGG - chr6.2:83508498-83508517 MS.gene05478:CDS 45.0%
GGTGGGAATATCGATACGTT+TGG - chr6.2:83508952-83508971 MS.gene05478:CDS 45.0%
GTCTATGGCTTATTGGGATC+AGG - chr6.2:83508606-83508625 MS.gene05478:CDS 45.0%
TACGTTTGGTCATTTGACCG+AGG - chr6.2:83508966-83508985 MS.gene05478:CDS 45.0%
TCCCCTCATACTCAAATCCT+CGG + chr6.2:83508986-83509005 None:intergenic 45.0%
TGACCGAGGATTTGAGTATG+AGG - chr6.2:83508980-83508999 MS.gene05478:CDS 45.0%
TGATCACCACCCATCTCATA+AGG + chr6.2:83508637-83508656 None:intergenic 45.0%
TGCATTGCTTTCGAAGAAGC+AGG - chr6.2:83509620-83509639 MS.gene05478:CDS 45.0%
TGTCCTCTTTATATGCCGGA+TGG - chr6.2:83508340-83508359 MS.gene05478:CDS 45.0%
TGTCTAGCAAGAACACCAAG+CGG + chr6.2:83508286-83508305 None:intergenic 45.0%
! ACCCTTTTGACAGACTCCAA+CGG + chr6.2:83509108-83509127 None:intergenic 45.0%
! ATGCATGGAGCATTCATGAG+TGG - chr6.2:83509351-83509370 MS.gene05478:CDS 45.0%
! GAGGGTCTTCCGATTTTCTA+TGG - chr6.2:83508703-83508722 MS.gene05478:CDS 45.0%
! GATTTTCTATGGGAGGACTG+TGG - chr6.2:83508714-83508733 MS.gene05478:CDS 45.0%
! GTCTTCCGATTTTCTATGGG+AGG - chr6.2:83508707-83508726 MS.gene05478:CDS 45.0%
! GTGTGATTGTTATTGGTGCC+GGG - chr6.2:83508071-83508090 MS.gene05478:CDS 45.0%
! TTGTTGCTGTTGTTGTTGGG+AGG + chr6.2:83507867-83507886 None:intergenic 45.0%
!! AAATGGTCTTGTCCATGGGA+AGG - chr6.2:83509800-83509819 MS.gene05478:CDS 45.0%
!! AGGATGTTTGATGGTGATGG+TGG - chr6.2:83508193-83508212 MS.gene05478:CDS 45.0%
!! AGGATTCGGGTTGCTGAATA+AGG - chr6.2:83508900-83508919 MS.gene05478:CDS 45.0%
!! ATGTTTGATGGTGATGGTGG+TGG - chr6.2:83508196-83508215 MS.gene05478:CDS 45.0%
!! GAATCGGAGGAATTTTGAGG+TGG + chr6.2:83507842-83507861 None:intergenic 45.0%
!! GCAGAAAATGGTCTTGTCCA+TGG - chr6.2:83509795-83509814 MS.gene05478:CDS 45.0%
!! TGAGGTTGATTCTAGGGTTG+AGG - chr6.2:83508378-83508397 MS.gene05478:CDS 45.0%
!! TTGGGTGGAAGTGTTCTTAC+TGG - chr6.2:83508247-83508266 MS.gene05478:CDS 45.0%
!!! CGTTTTTGCGGGTTTTGGAA+AGG + chr6.2:83507979-83507998 None:intergenic 45.0%
!!! CTTTAGCGACGTCGTTTTTG+CGG + chr6.2:83507991-83508010 None:intergenic 45.0%
!!! TTTAGCGACGTCGTTTTTGC+GGG + chr6.2:83507990-83508009 None:intergenic 45.0%
AAGCTCCCGAAATTCAGGTC+AGG + chr6.2:83509498-83509517 None:intergenic 50.0%
ACAAACGTCTCATTCCCTCC+AGG + chr6.2:83508670-83508689 None:intergenic 50.0%
CAGAGAAGCTCCCGAAATTC+AGG + chr6.2:83509503-83509522 None:intergenic 50.0%
CAGTACCCTGCAACAATGCA+TGG - chr6.2:83509336-83509355 MS.gene05478:CDS 50.0%
GCCGTTGGAGTCTGTCAAAA+GGG - chr6.2:83509104-83509123 MS.gene05478:CDS 50.0%
GCGCTTTGTACTGTGCCTTT+AGG - chr6.2:83508805-83508824 MS.gene05478:CDS 50.0%
GGTGTTAATGGGAATCCGCT+TGG - chr6.2:83508268-83508287 MS.gene05478:CDS 50.0%
TACCACGAAAACGACGTCGT+CGG - chr6.2:83507918-83507937 MS.gene05478:CDS 50.0%
TCAGGCAGTCTGTACTCGAT+GGG - chr6.2:83509185-83509204 MS.gene05478:CDS 50.0%
TCCGGAACAACAATCCCCTT+CGG + chr6.2:83509159-83509178 None:intergenic 50.0%
TCGAGTACAGACTGCCTGAA+CGG + chr6.2:83509184-83509203 None:intergenic 50.0%
TGAGACGTTTGTTCGCGTGT+TGG - chr6.2:83508678-83508697 MS.gene05478:CDS 50.0%
TTCAGGCAGTCTGTACTCGA+TGG - chr6.2:83509184-83509203 MS.gene05478:CDS 50.0%
TTCGTGGTAGGGTAGGGTTA+GGG + chr6.2:83507907-83507926 None:intergenic 50.0%
TTGTTGGGAGGATGAATCGG+AGG + chr6.2:83507855-83507874 None:intergenic 50.0%
TTTCGTGGTAGGGTAGGGTT+AGG + chr6.2:83507908-83507927 None:intergenic 50.0%
TTTGGAAGGTAGGGATCGAC+CGG - chr6.2:83508150-83508169 MS.gene05478:CDS 50.0%
! GAGTTTAGCTCAGTGTTGGG+AGG + chr6.2:83507707-83507726 None:intergenic 50.0%
! GGAGTCTGTCAAAAGGGTGT+TGG - chr6.2:83509110-83509129 MS.gene05478:CDS 50.0%
! GTTTGAGATGATGTCGCCGT+TGG - chr6.2:83509089-83509108 MS.gene05478:CDS 50.0%
! TCCGAAGGGGATTGTTGTTC+CGG - chr6.2:83509155-83509174 MS.gene05478:CDS 50.0%
! TGTTCCTTTAGGGACAGCGT+TGG - chr6.2:83508474-83508493 MS.gene05478:CDS 50.0%
!! ACGACGTCGTTTTCGTGGTA+GGG + chr6.2:83507918-83507937 None:intergenic 50.0%
!! AGAGAGTATTGGAGACGGGA+GGG - chr6.2:83509284-83509303 MS.gene05478:CDS 50.0%
!! GATTGTTATTGGTGCCGGGT+TGG - chr6.2:83508075-83508094 MS.gene05478:CDS 50.0%
!! GTCGTTTTCGTGGTAGGGTA+GGG + chr6.2:83507913-83507932 None:intergenic 50.0%
!! TTGATGGTGATGGTGGTGGT+GGG - chr6.2:83508200-83508219 MS.gene05478:CDS 50.0%
!! TTTGATGGTGATGGTGGTGG+TGG - chr6.2:83508199-83508218 MS.gene05478:CDS 50.0%
!! TTTGTTCGCGTGTTGGCTGA+GGG - chr6.2:83508685-83508704 MS.gene05478:CDS 50.0%
!!! GACGTCGTTTTTGCGGGTTT+TGG + chr6.2:83507984-83508003 None:intergenic 50.0%
AGCTATGCTTCTGTGTCCGG+AGG - chr6.2:83509024-83509043 MS.gene05478:CDS 55.0%
CAGGCAGTCTGTACTCGATG+GGG - chr6.2:83509186-83509205 MS.gene05478:CDS 55.0%
CGACAAGAGCCACAAGAAGC+GGG + chr6.2:83509050-83509069 None:intergenic 55.0%
CGAGTACAGACTGCCTGAAC+GGG + chr6.2:83509183-83509202 None:intergenic 55.0%
CGCCGTTGGAGTCTGTCAAA+AGG - chr6.2:83509103-83509122 MS.gene05478:CDS 55.0%
CTTCTTGTGGCTCTTGTCGC+CGG - chr6.2:83509051-83509070 MS.gene05478:CDS 55.0%
GATTCCGACGGTGGTTGTGA+CGG + chr6.2:83507776-83507795 None:intergenic 55.0%
GCGACAAGAGCCACAAGAAG+CGG + chr6.2:83509051-83509070 None:intergenic 55.0%
GGTAGGGTAGGGTTAGGGTT+TGG + chr6.2:83507902-83507921 None:intergenic 55.0%
GTGGGAGGGTTAAGACGAAG+AGG - chr6.2:83508173-83508192 MS.gene05478:CDS 55.0%
TGGGGTTGTGGTGATTCCGA+CGG + chr6.2:83507788-83507807 None:intergenic 55.0%
TGTGACGGTGGTTCCGATGA+GGG + chr6.2:83507761-83507780 None:intergenic 55.0%
TTCGTCTTAACCCTCCCACC+CGG + chr6.2:83508172-83508191 None:intergenic 55.0%
TTGGAAGGTAGGGATCGACC+GGG - chr6.2:83508151-83508170 MS.gene05478:CDS 55.0%
TTGTGACGGTGGTTCCGATG+AGG + chr6.2:83507762-83507781 None:intergenic 55.0%
! TCCTTTAGGGACAGCGTTGG+AGG - chr6.2:83508477-83508496 MS.gene05478:CDS 55.0%
! TGATGGGCAGCAGTTTCGTG+CGG - chr6.2:83508777-83508796 MS.gene05478:CDS 55.0%
!! AGCGTTGGAGGCGTTTCGTA+GGG - chr6.2:83508489-83508508 MS.gene05478:CDS 55.0%
!! CAGAGAGTATTGGAGACGGG+AGG - chr6.2:83509283-83509302 MS.gene05478:CDS 55.0%
!! CGTCGTTTTCGTGGTAGGGT+AGG + chr6.2:83507914-83507933 None:intergenic 55.0%
!! GACGACGTCGTTTTCGTGGT+AGG + chr6.2:83507919-83507938 None:intergenic 55.0%
!! GAGGGTGTTCTTTGCTGGAG+AGG - chr6.2:83509302-83509321 MS.gene05478:CDS 55.0%
!! GGGTGCTGGTGTGTACTGAT+GGG - chr6.2:83508761-83508780 MS.gene05478:CDS 55.0%
!! GTTTGTTCGCGTGTTGGCTG+AGG - chr6.2:83508684-83508703 MS.gene05478:CDS 55.0%
!! TGATGGTGATGGTGGTGGTG+GGG - chr6.2:83508201-83508220 MS.gene05478:CDS 55.0%
!! TGTTATTGGTGCCGGGTTGG+CGG - chr6.2:83508078-83508097 MS.gene05478:CDS 55.0%
!! TTCCGACGACGTCGTTTTCG+TGG + chr6.2:83507923-83507942 None:intergenic 55.0%
!!! ACGGTGTCGTTTTGGGGTTG+TGG + chr6.2:83507800-83507819 None:intergenic 55.0%
AAGGTAGGGATCGACCGGGT+GGG - chr6.2:83508155-83508174 MS.gene05478:CDS 60.0%
ACCACCGTCACAACCACCGT+CGG - chr6.2:83507769-83507788 MS.gene05478:CDS 60.0%
GCAGCAGTTTCGTGCGGACA+TGG - chr6.2:83508783-83508802 MS.gene05478:CDS 60.0%
GCCTCCAACGCTGTCCCTAA+AGG + chr6.2:83508481-83508500 None:intergenic 60.0%
GGTTGTGGTGATTCCGACGG+TGG + chr6.2:83507785-83507804 None:intergenic 60.0%
TCGCCGGAGAAGCTGCTCTT+AGG - chr6.2:83509067-83509086 MS.gene05478:CDS 60.0%
TGTTGTTCCGGAGCCCGTTC+AGG - chr6.2:83509167-83509186 MS.gene05478:CDS 60.0%
! CTTGTGGCTGCGGCGGATTT+GGG - chr6.2:83508229-83508248 MS.gene05478:CDS 60.0%
! GTACGGAAGTGATGGGGTGC+TGG - chr6.2:83508747-83508766 MS.gene05478:CDS 60.0%
! TCTTGTGGCTGCGGCGGATT+TGG - chr6.2:83508228-83508247 MS.gene05478:CDS 60.0%
!! CAGCGTTGGAGGCGTTTCGT+AGG - chr6.2:83508488-83508507 MS.gene05478:CDS 60.0%
!! GACGGGAGGGTGTTCTTTGC+TGG - chr6.2:83509297-83509316 MS.gene05478:CDS 60.0%
!! GGGGTGCTGGTGTGTACTGA+TGG - chr6.2:83508760-83508779 MS.gene05478:CDS 60.0%
!! GTTATTGGTGCCGGGTTGGC+GGG - chr6.2:83508079-83508098 MS.gene05478:CDS 60.0%
!! TGGGGATGGTCTTGTGGCTG+CGG - chr6.2:83508219-83508238 MS.gene05478:CDS 60.0%
!! TGGTGGTGGGGATGGTCTTG+TGG - chr6.2:83508213-83508232 MS.gene05478:CDS 60.0%
AGCCACAAGAAGCGGGCCTC+CGG + chr6.2:83509043-83509062 None:intergenic 65.0%
GAAGGTAGGGATCGACCGGG+TGG - chr6.2:83508154-83508173 MS.gene05478:CDS 65.0%
GGCAACCAAACCCGCCAACC+CGG + chr6.2:83508092-83508111 None:intergenic 65.0%
TAGGGATCGACCGGGTGGGA+GGG - chr6.2:83508159-83508178 MS.gene05478:CDS 65.0%
TCCGACGGTGGTTGTGACGG+TGG + chr6.2:83507773-83507792 None:intergenic 65.0%
! CAGACTGCCTGAACGGGCTC+CGG + chr6.2:83509177-83509196 None:intergenic 65.0%
!! GGATGGTCTTGTGGCTGCGG+CGG - chr6.2:83508222-83508241 MS.gene05478:CDS 65.0%
!! GGTGATGGTGGTGGTGGGGA+TGG - chr6.2:83508205-83508224 MS.gene05478:CDS 65.0%
GTAGGGATCGACCGGGTGGG+AGG - chr6.2:83508158-83508177 MS.gene05478:CDS 70.0%
GTCCGGAGGCCCGCTTCTTG+TGG - chr6.2:83509038-83509057 MS.gene05478:CDS 70.0%
! GTGGCTGCGGCGGATTTGGG+TGG - chr6.2:83508232-83508251 MS.gene05478:CDS 70.0%
!! TGGTGCCGGGTTGGCGGGTT+TGG - chr6.2:83508084-83508103 MS.gene05478:CDS 70.0%


Chromosome Type Strat End Strand Name
chr6.2 gene 83507695 83509845 83507695 ID=MS.gene05478
chr6.2 mRNA 83507695 83509845 83507695 ID=MS.gene05478.t1;Parent=MS.gene05478
chr6.2 exon 83507695 83509845 83507695 ID=MS.gene05478.t1.exon1;Parent=MS.gene05478.t1
chr6.2 CDS 83507695 83509845 83507695 ID=cds.MS.gene05478.t1;Parent=MS.gene05478.t1
Gene Sequence

>MS.gene05478

ATGCACTCACCTCCCAACACTGAGCTAAACTCAGAACAACCCATTGATACACAAATGTCAGAACCCTCATCGGAACCACCGTCACAACCACCGTCGGAATCACCACAACCCCAAAACGACACCGTAACAATCTCCCCTGAAAACCCACCTCAAAATTCCTCCGATTCATCCTCCCAACAACAACAGCAACAACAAGAACAAGATCCAAACCCTAACCCTACCCTACCACGAAAACGACGTCGTCGGAAGAAGTTCTTCACAGAATTAACCGCAACAACTTCCCTTTCCAAAACCCGCAAAAACGACGTCGCTAAAGACTGTGATGACGAAGCCCTGATTGCAATTTCAGTAGGGTTTCATGTAATTGAGAGAGGGAGTGTGATTGTTATTGGTGCCGGGTTGGCGGGTTTGGTTGCCGCGCGACAATTAGTGTTTTTAGGGTTTAAAGTTTGTATTTTGGAAGGTAGGGATCGACCGGGTGGGAGGGTTAAGACGAAGAGGATGTTTGATGGTGATGGTGGTGGTGGGGATGGTCTTGTGGCTGCGGCGGATTTGGGTGGAAGTGTTCTTACTGGTGTTAATGGGAATCCGCTTGGTGTTCTTGCTAGACAATTAGATTTGCCGCTTCATAAGGTTAGGGATGTTTGTCCTCTTTATATGCCGGATGGAAAATGTGTTGATTCTGAGGTTGATTCTAGGGTTGAGGTTTTGTTTAATAAGTTGTTAGAGAGGGTTTGTAAGCTTAGGCAAGCTATGATTGATGAGGTTAAGAGTGTTGATGTTCCTTTAGGGACAGCGTTGGAGGCGTTTCGTAGGGTTTATAAGGTTGCTGAGGATAAAGAAGAGAGGATGTTGTTGAATTGGCATCTTGCTAATTTGGAGCATGCAAATGCTACTCTTATGTCGAATTTGTCTATGGCTTATTGGGATCAGGATGATCCTTATGAGATGGGTGGTGATCATTGTTTCATTCCTGGAGGGAATGAGACGTTTGTTCGCGTGTTGGCTGAGGGTCTTCCGATTTTCTATGGGAGGACTGTGGAGTGTATTAAGTACGGAAGTGATGGGGTGCTGGTGTGTACTGATGGGCAGCAGTTTCGTGCGGACATGGCGCTTTGTACTGTGCCTTTAGGGGTTCTTAAGAAAGAATCGATTAAGTTTGTCCCTGATCTTCCTCAGAGTAAGAAGGATGCAATTCATAGGTTAGGATTCGGGTTGCTGAATAAGGTTGTGATGCTGTTTCCGACTAATTTTTGGGGTGGGAATATCGATACGTTTGGTCATTTGACCGAGGATTTGAGTATGAGGGGAGAGTTTTTTCTGTTCTATAGCTATGCTTCTGTGTCCGGAGGCCCGCTTCTTGTGGCTCTTGTCGCCGGAGAAGCTGCTCTTAGGTTTGAGATGATGTCGCCGTTGGAGTCTGTCAAAAGGGTGTTGGATGTTTTGAAGGATATTTTTCATCCGAAGGGGATTGTTGTTCCGGAGCCCGTTCAGGCAGTCTGTACTCGATGGGGAAAAGATGAATTCGCGTACGGATCTTATTCTTATGTTGCTGTTGGATCTTCAGGAGATGATTATGATATACTTTCAGAGAGTATTGGAGACGGGAGGGTGTTCTTTGCTGGAGAGGCAACTAGCAAACAGTACCCTGCAACAATGCATGGAGCATTCATGAGTGGAATGAGAGAAGCTGCAAACATTTTAAGAGTTACAAAACGGAAGTCTCAAAGTTCAATGCCATCTAACACAACAAAAAACATCGATGAGGAGACTGACGATTTAGACAAACTATTTGTGAAACCTGACCTGAATTTCGGGAGCTTCTCTGCTTTGTTTAATCCAAACCCGAATGATCTTGAATCTAGTTCAATACTTAGGGTTAAAATTGGAGGAGCTGTATCCAATTCCCCCAGTCTTTATCTTTATGCATTGCTTTCGAAGAAGCAGGTCATTGAGTTGAGTCAGATTGAAGGAGACGAAAATCGGATGAAAATGTTGAATCGCGATTTTGGGGTCAGTTTAGTTGGGAAAAAGGGTTTAAGTAGCACTGCTGAATCTCTTATTGCCAACATAAAGTTGCTGAGACCTCAGCTTAATGAAGCAGAAAATGGTCTTGTCCATGGGAAGGATAGTTGTATCATGGAAGAATAG
Protein sequence

>MS.gene05478.t1

MHSPPNTELNSEQPIDTQMSEPSSEPPSQPPSESPQPQNDTVTISPENPPQNSSDSSSQQQQQQQEQDPNPNPTLPRKRRRRKKFFTELTATTSLSKTRKNDVAKDCDDEALIAISVGFHVIERGSVIVIGAGLAGLVAARQLVFLGFKVCILEGRDRPGGRVKTKRMFDGDGGGGDGLVAAADLGGSVLTGVNGNPLGVLARQLDLPLHKVRDVCPLYMPDGKCVDSEVDSRVEVLFNKLLERVCKLRQAMIDEVKSVDVPLGTALEAFRRVYKVAEDKEERMLLNWHLANLEHANATLMSNLSMAYWDQDDPYEMGGDHCFIPGGNETFVRVLAEGLPIFYGRTVECIKYGSDGVLVCTDGQQFRADMALCTVPLGVLKKESIKFVPDLPQSKKDAIHRLGFGLLNKVVMLFPTNFWGGNIDTFGHLTEDLSMRGEFFLFYSYASVSGGPLLVALVAGEAALRFEMMSPLESVKRVLDVLKDIFHPKGIVVPEPVQAVCTRWGKDEFAYGSYSYVAVGSSGDDYDILSESIGDGRVFFAGEATSKQYPATMHGAFMSGMREAANILRVTKRKSQSSMPSNTTKNIDEETDDLDKLFVKPDLNFGSFSALFNPNPNDLESSSILRVKIGGAVSNSPSLYLYALLSKKQVIELSQIEGDENRMKMLNRDFGVSLVGKKGLSSTAESLIANIKLLRPQLNEAENGLVHGKDSCIMEE