Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066105.t1 | XP_013461938.1 | 99 | 104 | 1 | 0 | 1 | 104 | 429 | 532 | 5.80E-48 | 199.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066105.t1 | Q9LV21 | 92.3 | 104 | 8 | 0 | 1 | 104 | 433 | 536 | 2.9e-48 | 192.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066105.t1 | A0A2K3MWZ1 | 99.0 | 104 | 1 | 0 | 1 | 104 | 430 | 533 | 4.2e-48 | 199.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene066105 | 0.841018 | 6.13E-58 | -1.69E-46 |
| MS.gene049159 | MS.gene066105 | 0.855695 | 5.31E-62 | -1.69E-46 |
| MS.gene049254 | MS.gene066105 | 0.805725 | 1.16E-49 | -1.69E-46 |
| MS.gene049255 | MS.gene066105 | 0.853385 | 2.47E-61 | -1.69E-46 |
| MS.gene049341 | MS.gene066105 | 0.850273 | 1.89E-60 | -1.69E-46 |
| MS.gene049350 | MS.gene066105 | 0.810367 | 1.19E-50 | -1.69E-46 |
| MS.gene049404 | MS.gene066105 | 0.806617 | 7.54E-50 | -1.69E-46 |
| MS.gene049501 | MS.gene066105 | 0.837504 | 5.00E-57 | -1.69E-46 |
| MS.gene049672 | MS.gene066105 | 0.803526 | 3.34E-49 | -1.69E-46 |
| MS.gene049821 | MS.gene066105 | 0.873835 | 1.08E-67 | -1.69E-46 |
| MS.gene049825 | MS.gene066105 | 0.81677 | 4.66E-52 | -1.69E-46 |
| MS.gene049850 | MS.gene066105 | 0.864472 | 1.19E-64 | -1.69E-46 |
| MS.gene049873 | MS.gene066105 | 0.833309 | 5.74E-56 | -1.69E-46 |
| MS.gene049887 | MS.gene066105 | 0.802494 | 5.46E-49 | -1.69E-46 |
| MS.gene050020 | MS.gene066105 | 0.860197 | 2.44E-63 | -1.69E-46 |
| MS.gene050303 | MS.gene066105 | 0.810006 | 1.43E-50 | -1.69E-46 |
| MS.gene050442 | MS.gene066105 | 0.812978 | 3.23E-51 | -1.69E-46 |
| MS.gene05045 | MS.gene066105 | 0.830554 | 2.75E-55 | -1.69E-46 |
| MS.gene050689 | MS.gene066105 | 0.841409 | 4.84E-58 | -1.69E-46 |
| MS.gene050719 | MS.gene066105 | 0.801098 | 1.06E-48 | -1.69E-46 |
| MS.gene050721 | MS.gene066105 | 0.802504 | 5.44E-49 | -1.69E-46 |
| MS.gene050724 | MS.gene066105 | 0.807829 | 4.18E-50 | -1.69E-46 |
| MS.gene050807 | MS.gene066105 | 0.836454 | 9.27E-57 | -1.69E-46 |
| MS.gene050808 | MS.gene066105 | 0.809456 | 1.87E-50 | -1.69E-46 |
| MS.gene050815 | MS.gene066105 | 0.859925 | 2.95E-63 | -1.69E-46 |
| MS.gene050838 | MS.gene066105 | 0.809865 | 1.53E-50 | -1.69E-46 |
| MS.gene050844 | MS.gene066105 | 0.812485 | 4.14E-51 | -1.69E-46 |
| MS.gene050901 | MS.gene066105 | 0.831011 | 2.13E-55 | -1.69E-46 |
| MS.gene051018 | MS.gene066105 | 0.811335 | 7.37E-51 | -1.69E-46 |
| MS.gene051198 | MS.gene066105 | 0.807294 | 5.42E-50 | -1.69E-46 |
| MS.gene051243 | MS.gene066105 | 0.815165 | 1.06E-51 | -1.69E-46 |
| MS.gene051247 | MS.gene066105 | 0.86144 | 1.02E-63 | -1.69E-46 |
| MS.gene051328 | MS.gene066105 | 0.886057 | 4.76E-72 | -1.69E-46 |
| MS.gene05134 | MS.gene066105 | 0.847864 | 8.83E-60 | -1.69E-46 |
| MS.gene051388 | MS.gene066105 | 0.825829 | 3.79E-54 | -1.69E-46 |
| MS.gene05157 | MS.gene066105 | 0.857758 | 1.31E-62 | -1.69E-46 |
| MS.gene051700 | MS.gene066105 | 0.843775 | 1.14E-58 | -1.69E-46 |
| MS.gene051719 | MS.gene066105 | 0.873539 | 1.36E-67 | -1.69E-46 |
| MS.gene051720 | MS.gene066105 | 0.867169 | 1.67E-65 | -1.69E-46 |
| MS.gene051744 | MS.gene066105 | 0.846182 | 2.55E-59 | -1.69E-46 |
| MS.gene051791 | MS.gene066105 | 0.813063 | 3.09E-51 | -1.69E-46 |
| MS.gene05188 | MS.gene066105 | 0.810257 | 1.26E-50 | -1.69E-46 |
| MS.gene051957 | MS.gene066105 | 0.806515 | 7.93E-50 | -1.69E-46 |
| MS.gene052012 | MS.gene066105 | 0.812278 | 4.59E-51 | -1.69E-46 |
| MS.gene052020 | MS.gene066105 | 0.825477 | 4.59E-54 | -1.69E-46 |
| MS.gene052133 | MS.gene066105 | 0.864513 | 1.15E-64 | -1.69E-46 |
| MS.gene052459 | MS.gene066105 | 0.801701 | 7.95E-49 | -1.69E-46 |
| MS.gene052516 | MS.gene066105 | 0.869373 | 3.25E-66 | -1.69E-46 |
| MS.gene052519 | MS.gene066105 | 0.868815 | 4.93E-66 | -1.69E-46 |
| MS.gene052561 | MS.gene066105 | 0.803218 | 3.87E-49 | -1.69E-46 |
| MS.gene052582 | MS.gene066105 | 0.819511 | 1.12E-52 | -1.69E-46 |
| MS.gene052584 | MS.gene066105 | 0.836713 | 7.96E-57 | -1.69E-46 |
| MS.gene052611 | MS.gene066105 | 0.847895 | 8.66E-60 | -1.69E-46 |
| MS.gene052612 | MS.gene066105 | 0.800786 | 1.22E-48 | -1.69E-46 |
| MS.gene052665 | MS.gene066105 | 0.832451 | 9.38E-56 | -1.69E-46 |
| MS.gene052773 | MS.gene066105 | 0.828187 | 1.03E-54 | -1.69E-46 |
| MS.gene052863 | MS.gene066105 | 0.860096 | 2.62E-63 | -1.69E-46 |
| MS.gene052879 | MS.gene066105 | 0.83075 | 2.46E-55 | -1.69E-46 |
| MS.gene052880 | MS.gene066105 | 0.817552 | 3.11E-52 | -1.69E-46 |
| MS.gene052896 | MS.gene066105 | 0.840033 | 1.11E-57 | -1.69E-46 |
| MS.gene052947 | MS.gene066105 | 0.846335 | 2.32E-59 | -1.69E-46 |
| MS.gene053008 | MS.gene066105 | 0.849449 | 3.21E-60 | -1.69E-46 |
| MS.gene053122 | MS.gene066105 | 0.847438 | 1.16E-59 | -1.69E-46 |
| MS.gene053144 | MS.gene066105 | 0.845871 | 3.10E-59 | -1.69E-46 |
| MS.gene053226 | MS.gene066105 | 0.872611 | 2.78E-67 | -1.69E-46 |
| MS.gene053581 | MS.gene066105 | 0.849722 | 2.69E-60 | -1.69E-46 |
| MS.gene053599 | MS.gene066105 | 0.825959 | 3.53E-54 | -1.69E-46 |
| MS.gene05364 | MS.gene066105 | 0.81718 | 3.77E-52 | -1.69E-46 |
| MS.gene053883 | MS.gene066105 | 0.827916 | 1.20E-54 | -1.69E-46 |
| MS.gene054236 | MS.gene066105 | 0.810198 | 1.30E-50 | -1.69E-46 |
| MS.gene054472 | MS.gene066105 | 0.818318 | 2.09E-52 | -1.69E-46 |
| MS.gene054584 | MS.gene066105 | 0.843105 | 1.72E-58 | -1.69E-46 |
| MS.gene05462 | MS.gene066105 | 0.805833 | 1.10E-49 | -1.69E-46 |
| MS.gene054651 | MS.gene066105 | 0.839456 | 1.57E-57 | -1.69E-46 |
| MS.gene054775 | MS.gene066105 | 0.825971 | 3.50E-54 | -1.69E-46 |
| MS.gene05478 | MS.gene066105 | 0.805162 | 1.53E-49 | -1.69E-46 |
| MS.gene055144 | MS.gene066105 | 0.800492 | 1.40E-48 | -1.69E-46 |
| MS.gene055162 | MS.gene066105 | 0.810362 | 1.20E-50 | -1.69E-46 |
| MS.gene05517 | MS.gene066105 | 0.817678 | 2.91E-52 | -1.69E-46 |
| MS.gene055465 | MS.gene066105 | 0.874088 | 8.87E-68 | -1.69E-46 |
| MS.gene055466 | MS.gene066105 | 0.900248 | 8.85E-78 | -1.69E-46 |
| MS.gene055468 | MS.gene066105 | 0.894347 | 2.69E-75 | -1.69E-46 |
| MS.gene055469 | MS.gene066105 | 0.812388 | 4.35E-51 | -1.69E-46 |
| MS.gene055512 | MS.gene066105 | 0.860212 | 2.42E-63 | -1.69E-46 |
| MS.gene055513 | MS.gene066105 | 0.813423 | 2.58E-51 | -1.69E-46 |
| MS.gene055537 | MS.gene066105 | 0.815875 | 7.39E-52 | -1.69E-46 |
| MS.gene055555 | MS.gene066105 | 0.801187 | 1.01E-48 | -1.69E-46 |
| MS.gene055580 | MS.gene066105 | -0.882194 | 1.28E-70 | -1.69E-46 |
| MS.gene055608 | MS.gene066105 | 0.813186 | 2.91E-51 | -1.69E-46 |
| MS.gene055623 | MS.gene066105 | 0.805652 | 1.20E-49 | -1.69E-46 |
| MS.gene055778 | MS.gene066105 | 0.844352 | 7.98E-59 | -1.69E-46 |
| MS.gene055808 | MS.gene066105 | 0.820101 | 8.19E-53 | -1.69E-46 |
| MS.gene055818 | MS.gene066105 | 0.855303 | 6.90E-62 | -1.69E-46 |
| MS.gene055912 | MS.gene066105 | 0.812876 | 3.40E-51 | -1.69E-46 |
| MS.gene055979 | MS.gene066105 | 0.800313 | 1.53E-48 | -1.69E-46 |
| MS.gene056005 | MS.gene066105 | 0.825761 | 3.93E-54 | -1.69E-46 |
| MS.gene056043 | MS.gene066105 | -0.866328 | 3.09E-65 | -1.69E-46 |
| MS.gene056174 | MS.gene066105 | 0.826248 | 3.01E-54 | -1.69E-46 |
| MS.gene056215 | MS.gene066105 | 0.879482 | 1.20E-69 | -1.69E-46 |
| MS.gene056308 | MS.gene066105 | 0.835132 | 2.00E-56 | -1.69E-46 |
| MS.gene056366 | MS.gene066105 | 0.828039 | 1.12E-54 | -1.69E-46 |
| MS.gene056498 | MS.gene066105 | 0.803005 | 4.28E-49 | -1.69E-46 |
| MS.gene056545 | MS.gene066105 | 0.804635 | 1.97E-49 | -1.69E-46 |
| MS.gene056553 | MS.gene066105 | 0.832642 | 8.41E-56 | -1.69E-46 |
| MS.gene056595 | MS.gene066105 | 0.875061 | 4.15E-68 | -1.69E-46 |
| MS.gene056631 | MS.gene066105 | 0.816608 | 5.07E-52 | -1.69E-46 |
| MS.gene056760 | MS.gene066105 | 0.803847 | 2.87E-49 | -1.69E-46 |
| MS.gene057060 | MS.gene066105 | 0.8158 | 7.68E-52 | -1.69E-46 |
| MS.gene057140 | MS.gene066105 | 0.814523 | 1.48E-51 | -1.69E-46 |
| MS.gene057314 | MS.gene066105 | 0.84708 | 1.45E-59 | -1.69E-46 |
| MS.gene057444 | MS.gene066105 | 0.840905 | 6.56E-58 | -1.69E-46 |
| MS.gene057446 | MS.gene066105 | 0.808542 | 2.94E-50 | -1.69E-46 |
| MS.gene057693 | MS.gene066105 | 0.851974 | 6.26E-61 | -1.69E-46 |
| MS.gene057694 | MS.gene066105 | 0.80273 | 4.88E-49 | -1.69E-46 |
| MS.gene057705 | MS.gene066105 | 0.83042 | 2.97E-55 | -1.69E-46 |
| MS.gene057915 | MS.gene066105 | 0.848068 | 7.75E-60 | -1.69E-46 |
| MS.gene058009 | MS.gene066105 | 0.818421 | 1.98E-52 | -1.69E-46 |
| MS.gene058010 | MS.gene066105 | 0.816449 | 5.50E-52 | -1.69E-46 |
| MS.gene058037 | MS.gene066105 | 0.825987 | 3.47E-54 | -1.69E-46 |
| MS.gene058084 | MS.gene066105 | 0.841174 | 5.58E-58 | -1.69E-46 |
| MS.gene058216 | MS.gene066105 | 0.821561 | 3.78E-53 | -1.69E-46 |
| MS.gene058339 | MS.gene066105 | 0.806822 | 6.83E-50 | -1.69E-46 |
| MS.gene05847 | MS.gene066105 | 0.803738 | 3.02E-49 | -1.69E-46 |
| MS.gene058548 | MS.gene066105 | 0.801884 | 7.29E-49 | -1.69E-46 |
| MS.gene058625 | MS.gene066105 | 0.837477 | 5.08E-57 | -1.69E-46 |
| MS.gene058636 | MS.gene066105 | 0.820369 | 7.12E-53 | -1.69E-46 |
| MS.gene058783 | MS.gene066105 | 0.813925 | 2.00E-51 | -1.69E-46 |
| MS.gene058852 | MS.gene066105 | 0.804991 | 1.66E-49 | -1.69E-46 |
| MS.gene058906 | MS.gene066105 | 0.833903 | 4.08E-56 | -1.69E-46 |
| MS.gene059084 | MS.gene066105 | 0.802316 | 5.94E-49 | -1.69E-46 |
| MS.gene059151 | MS.gene066105 | 0.828652 | 7.98E-55 | -1.69E-46 |
| MS.gene05954 | MS.gene066105 | 0.83684 | 7.39E-57 | -1.69E-46 |
| MS.gene059625 | MS.gene066105 | 0.834924 | 2.26E-56 | -1.69E-46 |
| MS.gene05978 | MS.gene066105 | 0.823998 | 1.02E-53 | -1.69E-46 |
| MS.gene05979 | MS.gene066105 | 0.812269 | 4.61E-51 | -1.69E-46 |
| MS.gene059842 | MS.gene066105 | 0.86892 | 4.56E-66 | -1.69E-46 |
| MS.gene059937 | MS.gene066105 | 0.845406 | 4.14E-59 | -1.69E-46 |
| MS.gene06001 | MS.gene066105 | 0.827068 | 1.92E-54 | -1.69E-46 |
| MS.gene060194 | MS.gene066105 | 0.822689 | 2.07E-53 | -1.69E-46 |
| MS.gene060310 | MS.gene066105 | 0.814931 | 1.20E-51 | -1.69E-46 |
| MS.gene060322 | MS.gene066105 | 0.833089 | 6.51E-56 | -1.69E-46 |
| MS.gene060429 | MS.gene066105 | 0.913253 | 7.62E-84 | -1.69E-46 |
| MS.gene060519 | MS.gene066105 | 0.80234 | 5.88E-49 | -1.69E-46 |
| MS.gene060521 | MS.gene066105 | 0.817268 | 3.60E-52 | -1.69E-46 |
| MS.gene060587 | MS.gene066105 | 0.869435 | 3.10E-66 | -1.69E-46 |
| MS.gene060689 | MS.gene066105 | 0.817185 | 3.76E-52 | -1.69E-46 |
| MS.gene060803 | MS.gene066105 | 0.84023 | 9.86E-58 | -1.69E-46 |
| MS.gene060886 | MS.gene066105 | 0.801495 | 8.76E-49 | -1.69E-46 |
| MS.gene060907 | MS.gene066105 | 0.800825 | 1.20E-48 | -1.69E-46 |
| MS.gene060917 | MS.gene066105 | 0.850916 | 1.25E-60 | -1.69E-46 |
| MS.gene061100 | MS.gene066105 | 0.804182 | 2.44E-49 | -1.69E-46 |
| MS.gene061176 | MS.gene066105 | 0.833599 | 4.86E-56 | -1.69E-46 |
| MS.gene061177 | MS.gene066105 | 0.833712 | 4.56E-56 | -1.69E-46 |
| MS.gene061178 | MS.gene066105 | 0.833307 | 5.75E-56 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066105.t1 | MTR_3g109925 | 99.038 | 104 | 1 | 0 | 1 | 104 | 372 | 475 | 1.35e-69 | 215 |
| MS.gene066105.t1 | MTR_3g109925 | 99.038 | 104 | 1 | 0 | 1 | 104 | 429 | 532 | 3.10e-69 | 216 |
| MS.gene066105.t1 | MTR_8g105970 | 88.060 | 67 | 8 | 0 | 1 | 67 | 287 | 353 | 3.32e-37 | 128 |
| MS.gene066105.t1 | MTR_3g086330 | 38.614 | 101 | 61 | 1 | 3 | 102 | 431 | 531 | 5.36e-17 | 75.1 |
| MS.gene066105.t1 | MTR_7g113470 | 33.000 | 100 | 67 | 0 | 3 | 102 | 418 | 517 | 4.88e-15 | 69.3 |
| MS.gene066105.t1 | MTR_7g067470 | 32.000 | 100 | 68 | 0 | 3 | 102 | 418 | 517 | 1.98e-14 | 67.8 |
| MS.gene066105.t1 | MTR_1g030650 | 32.632 | 95 | 64 | 0 | 10 | 104 | 432 | 526 | 2.29e-13 | 64.7 |
| MS.gene066105.t1 | MTR_6g032870 | 33.684 | 95 | 63 | 0 | 10 | 104 | 429 | 523 | 5.49e-13 | 63.5 |
| MS.gene066105.t1 | MTR_2g013540 | 36.458 | 96 | 60 | 1 | 8 | 102 | 280 | 375 | 8.58e-11 | 57.4 |
| MS.gene066105.t1 | MTR_5g087560 | 33.000 | 100 | 64 | 2 | 5 | 101 | 427 | 526 | 8.85e-11 | 57.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066105.t1 | AT3G18190 | 92.308 | 104 | 8 | 0 | 1 | 104 | 433 | 536 | 1.29e-65 | 206 |
| MS.gene066105.t1 | AT1G24510 | 42.574 | 101 | 57 | 1 | 3 | 102 | 431 | 531 | 2.03e-18 | 79.3 |
| MS.gene066105.t1 | AT1G24510 | 42.574 | 101 | 57 | 1 | 3 | 102 | 378 | 478 | 2.31e-18 | 79.0 |
| MS.gene066105.t1 | AT1G24510 | 42.574 | 101 | 57 | 1 | 3 | 102 | 355 | 455 | 2.32e-18 | 79.0 |
| MS.gene066105.t1 | AT5G20890 | 34.694 | 98 | 64 | 0 | 5 | 102 | 420 | 517 | 2.86e-14 | 67.4 |
| MS.gene066105.t1 | AT3G03960 | 30.693 | 101 | 70 | 0 | 4 | 104 | 429 | 529 | 6.35e-13 | 63.5 |
| MS.gene066105.t1 | AT3G11830 | 33.000 | 100 | 64 | 2 | 5 | 101 | 425 | 524 | 3.14e-11 | 58.5 |
| MS.gene066105.t1 | AT3G11830 | 33.000 | 100 | 64 | 2 | 5 | 101 | 427 | 526 | 3.27e-11 | 58.5 |
Find 26 sgRNAs with CRISPR-Local
Find 28 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCATTCTCAGCAAGGGTATA+TGG | 0.307049 | 3.3:-90256973 | None:intergenic |
| CTGTCAAGGCAATGGGTGCT+TGG | 0.396464 | 3.3:+90256884 | None:intergenic |
| AGCTCAGTAACAATTGCAAT+CGG | 0.423035 | 3.3:-90257006 | None:intergenic |
| TGTCAAGGCAATGGGTGCTT+GGG | 0.443067 | 3.3:+90256885 | None:intergenic |
| GGCAATGGGTGCTTGGGCTA+AGG | 0.472620 | 3.3:+90256891 | None:intergenic |
| GAATGTGGTGCAGCCATTGC+TGG | 0.481414 | 3.3:+90257107 | MS.gene066105:CDS |
| TATACCCTTGCTGAGAATGC+AGG | 0.493053 | 3.3:+90256976 | MS.gene066105:CDS |
| CTGAGGAATCGTCATGCTAA+AGG | 0.500255 | 3.3:+90257027 | MS.gene066105:CDS |
| TCAACACTGGAATCAATGTC+AGG | 0.507995 | 3.3:+90257055 | MS.gene066105:CDS |
| CCTCACACACTCTGTTGCCA+AGG | 0.573445 | 3.3:-90257148 | None:intergenic |
| TGGAACAACTTCAAGTGCCT+CGG | 0.574575 | 3.3:-90256953 | None:intergenic |
| GCTCAGTAACAATTGCAATC+GGG | 0.575791 | 3.3:-90257005 | None:intergenic |
| GCACTTGTGCTAACCAGCAA+TGG | 0.592202 | 3.3:-90257120 | None:intergenic |
| TCAAACCTGCATTCTCAGCA+AGG | 0.593910 | 3.3:-90256981 | None:intergenic |
| CTGTATTCGTGCATTTGCCG+AGG | 0.594695 | 3.3:+90256936 | MS.gene066105:CDS |
| ACACTCTGTTGCCAAGGTGA+TGG | 0.598855 | 3.3:-90257142 | None:intergenic |
| GCTAAAGGTGAGATCAACAC+TGG | 0.604820 | 3.3:+90257042 | MS.gene066105:CDS |
| TTGCAATTGTTACTGAGCTG+AGG | 0.612866 | 3.3:+90257010 | MS.gene066105:CDS |
| GCTGCACCACATTCTCCTCA+AGG | 0.613760 | 3.3:-90257098 | None:intergenic |
| TAGCACAAGTGCCATCACCT+TGG | 0.618089 | 3.3:+90257131 | MS.gene066105:CDS |
| TCAAATTACAAACATCCTTG+AGG | 0.621720 | 3.3:+90257083 | MS.gene066105:CDS |
| ACTGGAATCAATGTCAGGAA+AGG | 0.625427 | 3.3:+90257060 | MS.gene066105:CDS |
| AAACATCCTTGAGGAGAATG+TGG | 0.628033 | 3.3:+90257092 | MS.gene066105:CDS |
| CCTTGGCAACAGAGTGTGTG+AGG | 0.652881 | 3.3:+90257148 | MS.gene066105:CDS |
| CAAACCTGCATTCTCAGCAA+GGG | 0.653006 | 3.3:-90256980 | None:intergenic |
| TTGATGATATTGTAACTGTG+AGG | 0.759737 | 3.3:+90257184 | MS.gene066105:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TCAAATTACAAACATCCTTG+AGG | + | chr3.3:90257083-90257102 | MS.gene066105:CDS | 30.0% | |
| ! | TTGATGATATTGTAACTGTG+AGG | + | chr3.3:90257184-90257203 | MS.gene066105:CDS | 30.0% |
| AGCTCAGTAACAATTGCAAT+CGG | - | chr3.3:90257009-90257028 | None:intergenic | 35.0% | |
| AAACATCCTTGAGGAGAATG+TGG | + | chr3.3:90257092-90257111 | MS.gene066105:CDS | 40.0% | |
| ACTGGAATCAATGTCAGGAA+AGG | + | chr3.3:90257060-90257079 | MS.gene066105:CDS | 40.0% | |
| GCTCAGTAACAATTGCAATC+GGG | - | chr3.3:90257008-90257027 | None:intergenic | 40.0% | |
| TCAACACTGGAATCAATGTC+AGG | + | chr3.3:90257055-90257074 | MS.gene066105:CDS | 40.0% | |
| TTGCAATTGTTACTGAGCTG+AGG | + | chr3.3:90257010-90257029 | MS.gene066105:CDS | 40.0% | |
| CAAACCTGCATTCTCAGCAA+GGG | - | chr3.3:90256983-90257002 | None:intergenic | 45.0% | |
| CTGAGGAATCGTCATGCTAA+AGG | + | chr3.3:90257027-90257046 | MS.gene066105:CDS | 45.0% | |
| GCTAAAGGTGAGATCAACAC+TGG | + | chr3.3:90257042-90257061 | MS.gene066105:CDS | 45.0% | |
| TATACCCTTGCTGAGAATGC+AGG | + | chr3.3:90256976-90256995 | MS.gene066105:CDS | 45.0% | |
| TCAAACCTGCATTCTCAGCA+AGG | - | chr3.3:90256984-90257003 | None:intergenic | 45.0% | |
| TGGAACAACTTCAAGTGCCT+CGG | - | chr3.3:90256956-90256975 | None:intergenic | 45.0% | |
| ! | AAGGTTTTGCATGGGATGGA+GGG | + | chr3.3:90256910-90256929 | MS.gene066105:CDS | 45.0% |
| ! | CTTGGGCTAAGGTTTTGCAT+GGG | + | chr3.3:90256902-90256921 | MS.gene066105:CDS | 45.0% |
| ! | GCATTCTCAGCAAGGGTATA+TGG | - | chr3.3:90256976-90256995 | None:intergenic | 45.0% |
| ! | TAAGGTTTTGCATGGGATGG+AGG | + | chr3.3:90256909-90256928 | MS.gene066105:CDS | 45.0% |
| ACACTCTGTTGCCAAGGTGA+TGG | - | chr3.3:90257145-90257164 | None:intergenic | 50.0% | |
| CTGTATTCGTGCATTTGCCG+AGG | + | chr3.3:90256936-90256955 | MS.gene066105:CDS | 50.0% | |
| GCACTTGTGCTAACCAGCAA+TGG | - | chr3.3:90257123-90257142 | None:intergenic | 50.0% | |
| TAGCACAAGTGCCATCACCT+TGG | + | chr3.3:90257131-90257150 | MS.gene066105:CDS | 50.0% | |
| ! | GCTTGGGCTAAGGTTTTGCA+TGG | + | chr3.3:90256901-90256920 | MS.gene066105:CDS | 50.0% |
| ! | GGCTAAGGTTTTGCATGGGA+TGG | + | chr3.3:90256906-90256925 | MS.gene066105:CDS | 50.0% |
| CCTCACACACTCTGTTGCCA+AGG | - | chr3.3:90257151-90257170 | None:intergenic | 55.0% | |
| CCTTGGCAACAGAGTGTGTG+AGG | + | chr3.3:90257148-90257167 | MS.gene066105:CDS | 55.0% | |
| GAATGTGGTGCAGCCATTGC+TGG | + | chr3.3:90257107-90257126 | MS.gene066105:CDS | 55.0% | |
| GCTGCACCACATTCTCCTCA+AGG | - | chr3.3:90257101-90257120 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 90256895 | 90257209 | 90256895 | ID=MS.gene066105 |
| chr3.3 | mRNA | 90256895 | 90257209 | 90256895 | ID=MS.gene066105.t1;Parent=MS.gene066105 |
| chr3.3 | exon | 90256895 | 90257209 | 90256895 | ID=MS.gene066105.t1.exon1;Parent=MS.gene066105.t1 |
| chr3.3 | CDS | 90256895 | 90257209 | 90256895 | ID=cds.MS.gene066105.t1;Parent=MS.gene066105.t1 |
| Gene Sequence |
| Protein sequence |
