AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene38102


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene38102.t1 MTR_5g098060 95.817 263 9 2 1 262 1 262 2.14e-176 486
MS.gene38102.t1 MTR_7g101080 40.237 169 90 4 33 195 26 189 1.45e-32 120
MS.gene38102.t1 MTR_2g090765 33.043 230 151 3 31 259 22 249 3.86e-27 105
MS.gene38102.t1 MTR_4g059720 35.789 190 111 4 8 194 8 189 8.27e-25 99.4
MS.gene38102.t1 MTR_4g059840 32.787 244 146 8 28 261 55 290 7.85e-23 95.1
MS.gene38102.t1 MTR_2g093990 36.667 150 90 2 48 194 41 188 3.87e-21 89.7
MS.gene38102.t1 MTR_4g059790 32.174 230 138 8 28 247 55 276 5.01e-21 90.1
MS.gene38102.t1 MTR_4g053380 29.796 245 152 7 28 262 59 293 6.80e-21 89.7
MS.gene38102.t1 MTR_7g075453 33.714 175 110 4 47 219 183 353 4.81e-20 89.0
MS.gene38102.t1 MTR_8g087470 32.086 187 106 5 22 190 56 239 6.60e-19 84.7
MS.gene38102.t1 MTR_2g017960 29.381 194 128 4 48 237 68 256 2.37e-18 82.8
MS.gene38102.t1 MTR_8g087460 31.551 187 107 5 22 190 56 239 2.39e-18 83.2
MS.gene38102.t1 MTR_2g017950 28.829 222 124 7 1 190 2 221 2.78e-18 82.8
MS.gene38102.t1 MTR_2g017970 30.244 205 129 5 48 243 68 267 2.79e-18 82.4
MS.gene38102.t1 MTR_8g087450 31.098 164 104 4 31 190 63 221 1.19e-17 80.9
MS.gene38102.t1 MTR_2g437700 34.395 157 96 3 46 197 193 347 1.71e-17 81.6
MS.gene38102.t1 MTR_2g093980 32.961 179 111 5 23 196 45 219 9.75e-17 78.2
MS.gene38102.t1 MTR_4g059780 29.843 191 112 4 26 196 52 240 5.63e-16 76.3
MS.gene38102.t1 MTR_2g093970 28.502 207 121 5 9 190 9 213 1.81e-15 74.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene38102.t1 AT2G04780 65.079 189 66 0 47 235 45 233 2.30e-77 235
MS.gene38102.t1 AT2G04780 65.079 189 66 0 47 235 45 233 2.30e-77 235
MS.gene38102.t1 AT5G44130 34.359 195 120 4 47 237 35 225 1.19e-31 117
MS.gene38102.t1 AT2G20520 36.444 225 125 6 16 224 1 223 4.22e-31 116
MS.gene38102.t1 AT1G03870 33.333 219 130 6 47 262 38 243 5.55e-31 115
MS.gene38102.t1 AT5G03170 36.842 171 104 3 37 204 26 195 1.02e-28 109
MS.gene38102.t1 AT5G60490 34.054 185 109 2 48 230 39 212 1.01e-22 94.0
MS.gene38102.t1 AT3G46550 34.694 196 113 7 22 211 187 373 2.92e-21 92.4
MS.gene38102.t1 AT3G60900 34.659 176 105 5 39 208 180 351 1.48e-18 84.7
MS.gene38102.t1 AT2G45470 32.889 225 133 5 29 240 170 389 1.31e-16 79.3

Find 65 sgRNAs with CRISPR-Local

Find 78 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGTGTTGTTTGCTTGGTTT+TGG 0.201552 5.1:-78375071 None:intergenic
AGATCGTCAGCTATGGAATT+TGG 0.201670 5.1:+78375565 MS.gene38102:CDS
AACCTTGTGTTGGCAACTTT+TGG 0.243612 5.1:+78375591 MS.gene38102:CDS
CCTTCAATCCACAAAAGTTT+TGG 0.254005 5.1:+78375041 MS.gene38102:CDS
TCAGCTGCAGGCGCAATTTC+AGG 0.267157 5.1:-78375468 None:intergenic
CTTCTTCAGTGTTGTTTGCT+TGG 0.268289 5.1:-78375077 None:intergenic
AGAGGGTGGACTTACGGTTT+TGG 0.270515 5.1:-78374911 None:intergenic
GATGCGAAGGAACTGTCTTT+TGG 0.299960 5.1:-78375117 None:intergenic
CTCCCAGCACTTTGGTCAGC+TGG 0.303486 5.1:-78375495 None:intergenic
GATCGTCAGCTATGGAATTT+GGG 0.308948 5.1:+78375566 MS.gene38102:CDS
GGAACTGTCAAAATTAATTC+AGG 0.309797 5.1:+78375312 MS.gene38102:CDS
CACTGATATTCCTCCTGTTC+CGG 0.318299 5.1:+78375431 MS.gene38102:CDS
GGAGCCGGAGTTGGACTCTC+GGG 0.338226 5.1:-78374937 None:intergenic
TTGAATTTCACCGATAATTC+CGG 0.349314 5.1:+78375291 MS.gene38102:CDS
AATCTCATCAGCTTTGAGTT+TGG 0.359351 5.1:-78375160 None:intergenic
TTTGACAGTTCCGGAATTAT+CGG 0.367204 5.1:-78375301 None:intergenic
GCCTCAACTCCTACATTTGC+CGG 0.376027 5.1:+78375255 MS.gene38102:CDS
GTGGGTGCAGGAGCCGGAAC+AGG 0.376931 5.1:-78375444 None:intergenic
TCAGTGAGGTTAACAAAATC+TGG 0.394471 5.1:-78374976 None:intergenic
TGCAATTTATCAAGTTGATA+AGG 0.410347 5.1:+78375383 MS.gene38102:CDS
TCAACTTGATAAATTGCAAC+AGG 0.410462 5.1:-78375378 None:intergenic
ACTGAATTACTCACCGTTGC+TGG 0.419071 5.1:+78374994 MS.gene38102:CDS
ATGTAGGAGTTGAGGCAGTT+TGG 0.424006 5.1:-78375248 None:intergenic
TGGAGCCGGAGTTGGACTCT+CGG 0.431321 5.1:-78374938 None:intergenic
TTGTAAGAAGACGAACTATC+TGG 0.433149 5.1:-78375543 None:intergenic
GCTGGTGCTGGAGCCGGAGT+TGG 0.434583 5.1:-78374946 None:intergenic
TATTGAAGAAAAGTGTGAAA+TGG 0.451994 5.1:-78375018 None:intergenic
GTAGGTGCTGGTGCTGGAGC+CGG 0.452561 5.1:-78374952 None:intergenic
TCAGGTGTGGGTGCAGGAGC+CGG 0.468971 5.1:-78375450 None:intergenic
TCTCCAGCTGACCAAAGTGC+TGG 0.473142 5.1:+78375492 MS.gene38102:CDS
GCAATTTCAGGTGTGGGTGC+AGG 0.479601 5.1:-78375456 None:intergenic
GAGCCGGAGTTGGACTCTCG+GGG 0.491727 5.1:-78374936 None:intergenic
TGCAGGCGCAATTTCAGGTG+TGG 0.492103 5.1:-78375463 None:intergenic
CAAGCAAACAACACTGAAGA+AGG 0.495908 5.1:+78375078 MS.gene38102:CDS
AATGTGGCAAAGCATGGAAA+AGG 0.503046 5.1:-78375194 None:intergenic
ACGATTTGCTCCCAGCACTT+TGG 0.511706 5.1:-78375503 None:intergenic
GAGTTGAGGCAGTTTGGCTA+AGG 0.512356 5.1:-78375242 None:intergenic
GCAGGCGCAATTTCAGGTGT+GGG 0.518557 5.1:-78375462 None:intergenic
TCTGGTGTAGGTGCTGGTGC+TGG 0.520921 5.1:-78374958 None:intergenic
TCAGCTGGAGAATCAGCTGC+AGG 0.533442 5.1:-78375480 None:intergenic
TCTTACAAGATCGTCAGCTA+TGG 0.536563 5.1:+78375558 MS.gene38102:CDS
CTCCAGCTGACCAAAGTGCT+GGG 0.544197 5.1:+78375493 MS.gene38102:CDS
AATAGAAATGTGGCAAAGCA+TGG 0.549806 5.1:-78375200 None:intergenic
TTCAGGATGGTCAATCACTA+AGG 0.555642 5.1:+78375329 MS.gene38102:CDS
GGTGCAGGAGCCGGAACAGG+AGG 0.561308 5.1:-78375441 None:intergenic
AGGTTAACAAAATCTGGTGT+AGG 0.563929 5.1:-78374970 None:intergenic
TAATCACCACCGGCAAATGT+AGG 0.566308 5.1:-78375264 None:intergenic
GCTTTGAGTTTGGATAAAGA+AGG 0.566467 5.1:-78375150 None:intergenic
ACAAAATCTGGTGTAGGTGC+TGG 0.568282 5.1:-78374964 None:intergenic
GAACTATCTGGTGAAGATGA+AGG 0.574002 5.1:-78375531 None:intergenic
TCTGCTGCTTCTGAGCTCAT+CGG 0.586497 5.1:+78374882 MS.gene38102:CDS
AACCAAAAGTTGCCAACACA+AGG 0.587265 5.1:-78375593 None:intergenic
AGGTTTCTTCAATGATGCGA+AGG 0.588628 5.1:-78375130 None:intergenic
CTGTCAAAATTAATTCAGGA+TGG 0.599822 5.1:+78375316 MS.gene38102:CDS
GCCGGAGTTGGACTCTCGGG+GGG 0.600157 5.1:-78374934 None:intergenic
AGTGTGAAATGGACCAGCAA+CGG 0.600472 5.1:-78375007 None:intergenic
ACCGGCAAATGTAGGAGTTG+AGG 0.606038 5.1:-78375256 None:intergenic
ATTCAAAGTGTAATCACCAC+CGG 0.611570 5.1:-78375274 None:intergenic
ATAAATTGCAACAGGATCAG+TGG 0.614239 5.1:-78375370 None:intergenic
AGCCGGAGTTGGACTCTCGG+GGG 0.617688 5.1:-78374935 None:intergenic
AATTTGGGGCAACCTTGTGT+TGG 0.628436 5.1:+78375581 MS.gene38102:CDS
ATCGTCAGCTATGGAATTTG+GGG 0.659891 5.1:+78375567 MS.gene38102:CDS
TCAGCTAGTGAATAGAAATG+TGG 0.662953 5.1:-78375210 None:intergenic
TCAACTCCTACATTTGCCGG+TGG 0.674619 5.1:+78375258 MS.gene38102:CDS
CAACGGTGAGTAATTCAGTG+AGG 0.749189 5.1:-78374990 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATTGAAGAAAAGTGTGAAA+TGG - chr5.1:78375021-78375040 None:intergenic 25.0%
!!! TGAATTAATTTTGACAGTTC+CGG - chr5.1:78375313-78375332 None:intergenic 25.0%
!!! TGCAATTTATCAAGTTGATA+AGG + chr5.1:78375383-78375402 MS.gene38102:CDS 25.0%
CTGTCAAAATTAATTCAGGA+TGG + chr5.1:78375316-78375335 MS.gene38102:CDS 30.0%
GGAACTGTCAAAATTAATTC+AGG + chr5.1:78375312-78375331 MS.gene38102:CDS 30.0%
TCAACTTGATAAATTGCAAC+AGG - chr5.1:78375381-78375400 None:intergenic 30.0%
TTGAATTTCACCGATAATTC+CGG + chr5.1:78375291-78375310 MS.gene38102:CDS 30.0%
AATAGAAATGTGGCAAAGCA+TGG - chr5.1:78375203-78375222 None:intergenic 35.0%
AGGTTAACAAAATCTGGTGT+AGG - chr5.1:78374973-78374992 None:intergenic 35.0%
ATAAATTGCAACAGGATCAG+TGG - chr5.1:78375373-78375392 None:intergenic 35.0%
ATTCAAAGTGTAATCACCAC+CGG - chr5.1:78375277-78375296 None:intergenic 35.0%
TCAGCTAGTGAATAGAAATG+TGG - chr5.1:78375213-78375232 None:intergenic 35.0%
TCAGTGAGGTTAACAAAATC+TGG - chr5.1:78374979-78374998 None:intergenic 35.0%
TTGTAAGAAGACGAACTATC+TGG - chr5.1:78375546-78375565 None:intergenic 35.0%
TTTGACAGTTCCGGAATTAT+CGG - chr5.1:78375304-78375323 None:intergenic 35.0%
! CCTTCAATCCACAAAAGTTT+TGG + chr5.1:78375041-78375060 MS.gene38102:CDS 35.0%
! GCTTTGAGTTTGGATAAAGA+AGG - chr5.1:78375153-78375172 None:intergenic 35.0%
!! AATCTCATCAGCTTTGAGTT+TGG - chr5.1:78375163-78375182 None:intergenic 35.0%
!! CCAAAACTTTTGTGGATTGA+AGG - chr5.1:78375044-78375063 None:intergenic 35.0%
AACCAAAAGTTGCCAACACA+AGG - chr5.1:78375596-78375615 None:intergenic 40.0%
AATGTGGCAAAGCATGGAAA+AGG - chr5.1:78375197-78375216 None:intergenic 40.0%
AGATCGTCAGCTATGGAATT+TGG + chr5.1:78375565-78375584 MS.gene38102:CDS 40.0%
AGGTTTCTTCAATGATGCGA+AGG - chr5.1:78375133-78375152 None:intergenic 40.0%
ATCGTCAGCTATGGAATTTG+GGG + chr5.1:78375567-78375586 MS.gene38102:CDS 40.0%
CAAGCAAACAACACTGAAGA+AGG + chr5.1:78375078-78375097 MS.gene38102:CDS 40.0%
GAACTATCTGGTGAAGATGA+AGG - chr5.1:78375534-78375553 None:intergenic 40.0%
GATCGTCAGCTATGGAATTT+GGG + chr5.1:78375566-78375585 MS.gene38102:CDS 40.0%
TCTTACAAGATCGTCAGCTA+TGG + chr5.1:78375558-78375577 MS.gene38102:CDS 40.0%
TTCAGGATGGTCAATCACTA+AGG + chr5.1:78375329-78375348 MS.gene38102:CDS 40.0%
! GAACGTGTCCAAAACTTTTG+TGG - chr5.1:78375052-78375071 None:intergenic 40.0%
! TGATAAGGTTTTGCTTCCTG+AGG + chr5.1:78375398-78375417 MS.gene38102:CDS 40.0%
!! AACCTTGTGTTGGCAACTTT+TGG + chr5.1:78375591-78375610 MS.gene38102:CDS 40.0%
!! CTTCTTCAGTGTTGTTTGCT+TGG - chr5.1:78375080-78375099 None:intergenic 40.0%
!! TTGCTTCCTGAGGCTATTTT+TGG + chr5.1:78375408-78375427 MS.gene38102:CDS 40.0%
!!! CAGTGTTGTTTGCTTGGTTT+TGG - chr5.1:78375074-78375093 None:intergenic 40.0%
!!! GTTGGCAACTTTTGGTTTGT+TGG + chr5.1:78375599-78375618 MS.gene38102:CDS 40.0%
ACAAAATCTGGTGTAGGTGC+TGG - chr5.1:78374967-78374986 None:intergenic 45.0%
ACTGAATTACTCACCGTTGC+TGG + chr5.1:78374994-78375013 MS.gene38102:CDS 45.0%
AGTGTGAAATGGACCAGCAA+CGG - chr5.1:78375010-78375029 None:intergenic 45.0%
ATGTAGGAGTTGAGGCAGTT+TGG - chr5.1:78375251-78375270 None:intergenic 45.0%
CAACGGTGAGTAATTCAGTG+AGG - chr5.1:78374993-78375012 None:intergenic 45.0%
CACTGATATTCCTCCTGTTC+CGG + chr5.1:78375431-78375450 MS.gene38102:CDS 45.0%
TAATCACCACCGGCAAATGT+AGG - chr5.1:78375267-78375286 None:intergenic 45.0%
TCAGTGCCAAAAATAGCCTC+AGG - chr5.1:78375417-78375436 None:intergenic 45.0%
! AATTTGGGGCAACCTTGTGT+TGG + chr5.1:78375581-78375600 MS.gene38102:CDS 45.0%
! GATGCGAAGGAACTGTCTTT+TGG - chr5.1:78375120-78375139 None:intergenic 45.0%
!!! GGCAACTTTTGGTTTGTTGG+TGG + chr5.1:78375602-78375621 MS.gene38102:CDS 45.0%
ACCGGCAAATGTAGGAGTTG+AGG - chr5.1:78375259-78375278 None:intergenic 50.0%
GAGTTGAGGCAGTTTGGCTA+AGG - chr5.1:78375245-78375264 None:intergenic 50.0%
GCCTCAACTCCTACATTTGC+CGG + chr5.1:78375255-78375274 MS.gene38102:CDS 50.0%
TCAACTCCTACATTTGCCGG+TGG + chr5.1:78375258-78375277 MS.gene38102:CDS 50.0%
! ACGATTTGCTCCCAGCACTT+TGG - chr5.1:78375506-78375525 None:intergenic 50.0%
! TCTGCTGCTTCTGAGCTCAT+CGG + chr5.1:78374882-78374901 MS.gene38102:CDS 50.0%
!! AGAGGGTGGACTTACGGTTT+TGG - chr5.1:78374914-78374933 None:intergenic 50.0%
TCAGCTGCAGGCGCAATTTC+AGG - chr5.1:78375471-78375490 None:intergenic 55.0%
TCAGCTGGAGAATCAGCTGC+AGG - chr5.1:78375483-78375502 None:intergenic 55.0%
TCTCCAGCTGACCAAAGTGC+TGG + chr5.1:78375492-78375511 MS.gene38102:CDS 55.0%
! CTCCAGCTGACCAAAGTGCT+GGG + chr5.1:78375493-78375512 MS.gene38102:CDS 55.0%
! GCAATTTCAGGTGTGGGTGC+AGG - chr5.1:78375459-78375478 None:intergenic 55.0%
! GCAGGCGCAATTTCAGGTGT+GGG - chr5.1:78375465-78375484 None:intergenic 55.0%
! TGCAGGCGCAATTTCAGGTG+TGG - chr5.1:78375466-78375485 None:intergenic 55.0%
CTCCCAGCACTTTGGTCAGC+TGG - chr5.1:78375498-78375517 None:intergenic 60.0%
TGGAGCCGGAGTTGGACTCT+CGG - chr5.1:78374941-78374960 None:intergenic 60.0%
!! TCTGGTGTAGGTGCTGGTGC+TGG - chr5.1:78374961-78374980 None:intergenic 60.0%
AGCCGGAGTTGGACTCTCGG+GGG - chr5.1:78374938-78374957 None:intergenic 65.0%
AGTTGGACTCTCGGGGGGAG+AGG - chr5.1:78374932-78374951 None:intergenic 65.0%
GAGCCGGAGTTGGACTCTCG+GGG - chr5.1:78374939-78374958 None:intergenic 65.0%
GGAGCCGGAGTTGGACTCTC+GGG - chr5.1:78374940-78374959 None:intergenic 65.0%
GTTGGACTCTCGGGGGGAGA+GGG - chr5.1:78374931-78374950 None:intergenic 65.0%
TCAGGTGTGGGTGCAGGAGC+CGG - chr5.1:78375453-78375472 None:intergenic 65.0%
TCCCCCCGAGAGTCCAACTC+CGG + chr5.1:78374933-78374952 MS.gene38102:CDS 65.0%
! GGGGGGAGAGGGTGGACTTA+CGG - chr5.1:78374920-78374939 None:intergenic 65.0%
!! GTAGGTGCTGGTGCTGGAGC+CGG - chr5.1:78374955-78374974 None:intergenic 65.0%
GCCGGAGTTGGACTCTCGGG+GGG - chr5.1:78374937-78374956 None:intergenic 70.0%
GGTGCAGGAGCCGGAACAGG+AGG - chr5.1:78375444-78375463 None:intergenic 70.0%
GTGGGTGCAGGAGCCGGAAC+AGG - chr5.1:78375447-78375466 None:intergenic 70.0%
! GCTGGTGCTGGAGCCGGAGT+TGG - chr5.1:78374949-78374968 None:intergenic 70.0%
GGACTCTCGGGGGGAGAGGG+TGG - chr5.1:78374928-78374947 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 78374847 78375635 78374847 ID=MS.gene38102
chr5.1 mRNA 78374847 78375635 78374847 ID=MS.gene38102.t1;Parent=MS.gene38102
chr5.1 exon 78374847 78375635 78374847 ID=MS.gene38102.t1.exon1;Parent=MS.gene38102.t1
chr5.1 CDS 78374847 78375635 78374847 ID=cds.MS.gene38102.t1;Parent=MS.gene38102.t1
Gene Sequence

>MS.gene38102

ATGAAGGTTTCATTGATTTTTCTTGTGAGCTGCACTCTGCTGCTTCTGAGCTCATCGGCATTTGCCAAAACCGTAAGTCCACCCTCTCCCCCCGAGAGTCCAACTCCGGCTCCAGCACCAGCACCTACACCAGATTTTGTTAACCTCACTGAATTACTCACCGTTGCTGGTCCATTTCACACTTTTCTTCAATACCTTCAATCCACAAAAGTTTTGGACACGTTCCAAAACCAAGCAAACAACACTGAAGAAGGAATCACTATCTTTGTTCCAAAAGACAGTTCCTTCGCATCATTGAAGAAACCTTCTTTATCCAAACTCAAAGCTGATGAGATTAAACAAGTGATCCTTTTCCATGCTTTGCCACATTTCTATTCACTAGCTGATTTCAAAAACCTTAGCCAAACTGCCTCAACTCCTACATTTGCCGGTGGTGATTACACTTTGAATTTCACCGATAATTCCGGAACTGTCAAAATTAATTCAGGATGGTCAATCACTAAGGTGACTAGTGCAGTTCATGCCACTGATCCTGTTGCAATTTATCAAGTTGATAAGGTTTTGCTTCCTGAGGCTATTTTTGGCACTGATATTCCTCCTGTTCCGGCTCCTGCACCCACACCTGAAATTGCGCCTGCAGCTGATTCTCCAGCTGACCAAAGTGCTGGGAGCAAATCGTCTTCACCTTCATCTTCACCAGATAGTTCGTCTTCTTACAAGATCGTCAGCTATGGAATTTGGGGCAACCTTGTGTTGGCAACTTTTGGTTTGTTGGTGGTACTCTTGTGA

Protein sequence

>MS.gene38102.t1

MKVSLIFLVSCTLLLLSSSAFAKTVSPPSPPESPTPAPAPAPTPDFVNLTELLTVAGPFHTFLQYLQSTKVLDTFQNQANNTEEGITIFVPKDSSFASLKKPSLSKLKADEIKQVILFHALPHFYSLADFKNLSQTASTPTFAGGDYTLNFTDNSGTVKINSGWSITKVTSAVHATDPVAIYQVDKVLLPEAIFGTDIPPVPAPAPTPEIAPAADSPADQSAGSKSSSPSSSPDSSSSYKIVSYGIWGNLVLATFGLLVVLL