AlfalfaGEDB | MS.gene81957

M. sativa cultivar XinJiangDaYe / MS.gene81957

KEGG: K18857
GO: GO:0003674 GO:0003824 GO:0005488 GO:0008150 GO:0008152 GO:0008270 GO:0016491 GO:0043167 GO:0043169 GO:0046872 GO:0046914 GO:0055114
PFAM: PF00107.29 PF08240.15

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene81957.t1	XP_013461377.1	98.2	380	7	0	1	380	1	380	1.00E-219	772.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene81957.t1	P12886	95.3	380	18	0	1	380	1	380	4.1e-217	755.0

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene81957.t1	A0A072V0U2	98.2	380	7	0	1	380	1	380	7.4e-220	772.3

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049137	MS.gene81957	-0.829504	4.96E-55	-1.69E-46
MS.gene049222	MS.gene81957	-0.820755	5.80E-53	-1.69E-46
MS.gene050747	MS.gene81957	-0.838361	3.01E-57	-1.69E-46
MS.gene050950	MS.gene81957	-0.836044	1.18E-56	-1.69E-46
MS.gene051387	MS.gene81957	-0.814967	1.18E-51	-1.69E-46
MS.gene051552	MS.gene81957	0.807666	4.52E-50	-1.69E-46
MS.gene051694	MS.gene81957	-0.820371	7.11E-53	-1.69E-46
MS.gene05234	MS.gene81957	0.810225	1.28E-50	-1.69E-46
MS.gene052501	MS.gene81957	-0.800716	1.26E-48	-1.69E-46
MS.gene052505	MS.gene81957	-0.808006	3.83E-50	-1.69E-46
MS.gene052606	MS.gene81957	0.806583	7.67E-50	-1.69E-46
MS.gene052615	MS.gene81957	-0.800446	1.44E-48	-1.69E-46
MS.gene052823	MS.gene81957	-0.803105	4.08E-49	-1.69E-46
MS.gene052825	MS.gene81957	-0.806805	6.88E-50	-1.69E-46
MS.gene053108	MS.gene81957	0.823391	1.42E-53	-1.69E-46
MS.gene053203	MS.gene81957	-0.804715	1.89E-49	-1.69E-46
MS.gene053421	MS.gene81957	0.879738	9.75E-70	-1.69E-46
MS.gene053579	MS.gene81957	-0.810719	1.00E-50	-1.69E-46
MS.gene053873	MS.gene81957	-0.832585	8.69E-56	-1.69E-46
MS.gene054884	MS.gene81957	0.803429	3.50E-49	-1.69E-46
MS.gene055158	MS.gene81957	0.839718	1.34E-57	-1.69E-46
MS.gene055159	MS.gene81957	0.821266	4.42E-53	-1.69E-46
MS.gene055577	MS.gene81957	-0.804644	1.96E-49	-1.69E-46
MS.gene056040	MS.gene81957	-0.820065	8.35E-53	-1.69E-46
MS.gene056514	MS.gene81957	-0.801683	8.02E-49	-1.69E-46
MS.gene056551	MS.gene81957	0.802517	5.40E-49	-1.69E-46
MS.gene056647	MS.gene81957	0.822865	1.88E-53	-1.69E-46
MS.gene057658	MS.gene81957	-0.811036	8.56E-51	-1.69E-46
MS.gene057831	MS.gene81957	-0.803176	3.95E-49	-1.69E-46
MS.gene058157	MS.gene81957	-0.80335	3.64E-49	-1.69E-46
MS.gene058170	MS.gene81957	0.810281	1.25E-50	-1.69E-46
MS.gene058174	MS.gene81957	0.812271	4.61E-51	-1.69E-46
MS.gene05837	MS.gene81957	-0.807109	5.93E-50	-1.69E-46
MS.gene058418	MS.gene81957	0.816905	4.34E-52	-1.69E-46
MS.gene059023	MS.gene81957	-0.801391	9.20E-49	-1.69E-46
MS.gene059046	MS.gene81957	0.805439	1.33E-49	-1.69E-46
MS.gene059303	MS.gene81957	-0.825291	5.08E-54	-1.69E-46
MS.gene059526	MS.gene81957	0.803465	3.44E-49	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene81957	MS.gene70145	PPI
MS.gene81957	MS.gene069804	PPI
MS.gene81957	MS.gene000485	PPI
MS.gene81957	MS.gene70056	PPI
MS.gene81957	MS.gene89779	PPI
MS.gene015154	MS.gene81957	PPI
MS.gene037523	MS.gene81957	PPI
MS.gene81957	MS.gene042841	PPI
MS.gene81957	MS.gene73688	PPI
MS.gene81957	MS.gene49741	PPI
MS.gene99200	MS.gene81957	PPI
MS.gene047628	MS.gene81957	PPI
MS.gene81957	MS.gene011817	PPI
MS.gene56778	MS.gene81957	PPI
MS.gene81957	MS.gene95648	PPI
MS.gene81957	MS.gene79768	PPI
MS.gene041083	MS.gene81957	PPI
MS.gene81957	MS.gene83309	PPI
MS.gene81957	MS.gene77397	PPI
MS.gene50792	MS.gene81957	PPI
MS.gene81957	MS.gene77398	PPI
MS.gene81957	MS.gene90510	PPI
MS.gene010354	MS.gene81957	PPI
MS.gene069033	MS.gene81957	PPI
MS.gene81957	MS.gene000484	PPI
MS.gene030545	MS.gene81957	PPI
MS.gene81957	MS.gene95649	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene81957.t1	MTR_3g089977	98.158	380	7	0	1	380	1	380	0.0	775
MS.gene81957.t1	MTR_3g089940	97.368	380	10	0	1	380	1	380	0.0	769
MS.gene81957.t1	MTR_3g089940	97.368	380	10	0	1	380	1	380	0.0	767
MS.gene81957.t1	MTR_3g089970	95.801	381	15	1	1	380	1	381	0.0	753
MS.gene81957.t1	MTR_3g089940	97.368	342	9	0	1	342	1	342	0.0	694
MS.gene81957.t1	MTR_3g089940	97.368	342	9	0	1	342	1	342	0.0	691
MS.gene81957.t1	MTR_7g099040	58.090	377	158	0	2	378	3	379	2.83e-169	478
MS.gene81957.t1	MTR_1g069430	56.267	375	164	0	4	378	3	377	1.91e-163	463
MS.gene81957.t1	MTR_6g068930	50.663	377	182	2	2	378	3	375	3.69e-141	406
MS.gene81957.t1	MTR_7g068320	48.303	383	192	5	1	378	12	393	3.46e-126	369
MS.gene81957.t1	MTR_7g068330	48.294	381	192	4	2	378	6	385	7.63e-126	367
MS.gene81957.t1	MTR_3g099460	49.471	378	185	4	6	378	13	389	1.45e-119	352
MS.gene81957.t1	MTR_5g087710	44.920	374	204	2	7	378	16	389	2.20e-118	349
MS.gene81957.t1	MTR_3g099460	48.942	378	180	5	6	378	13	382	2.56e-115	341
MS.gene81957.t1	MTR_3g079180	42.480	379	216	2	2	378	6	384	1.81e-108	323
MS.gene81957.t1	MTR_3g099460	48.910	321	160	3	61	378	7	326	1.45e-95	288
MS.gene81957.t1	MTR_5g045710	39.211	380	223	6	1	378	1	374	8.82e-89	273
MS.gene81957.t1	MTR_0001s0660	39.257	377	219	5	5	378	4	373	9.66e-89	275
MS.gene81957.t1	MTR_3g099460	51.590	283	131	4	6	283	13	294	1.08e-83	258
MS.gene81957.t1	MTR_3g099460	50.883	283	126	5	6	283	13	287	1.78e-79	247
MS.gene81957.t1	MTR_3g079170	41.455	275	139	4	101	370	25	282	5.72e-68	216
MS.gene81957.t1	MTR_5g045710	36.735	294	182	3	86	378	1	291	2.58e-61	199
MS.gene81957.t1	MTR_8g095220	31.316	380	238	15	12	378	55	424	1.15e-43	157
MS.gene81957.t1	MTR_8g095220	30.859	256	163	8	12	258	55	305	1.64e-30	119
MS.gene81957.t1	MTR_6g465010	25.405	370	224	13	16	369	3	336	2.19e-22	97.4
MS.gene81957.t1	MTR_6g466320	25.405	370	224	13	16	369	19	352	2.65e-22	97.4
MS.gene81957.t1	MTR_6g465010	25.405	370	224	13	16	369	19	352	2.65e-22	97.4
MS.gene81957.t1	MTR_5g031390	24.642	349	224	10	37	379	39	354	4.20e-13	70.1
MS.gene81957.t1	MTR_5g031190	23.714	350	226	13	37	379	39	354	8.12e-13	69.3
MS.gene81957.t1	MTR_4g115300	25.714	350	219	11	37	379	39	354	3.60e-12	67.4
MS.gene81957.t1	MTR_5g031210	24.277	346	229	11	37	379	39	354	1.08e-11	65.9
MS.gene81957.t1	MTR_5g031180	22.995	374	252	13	12	379	11	354	6.08e-11	63.5
MS.gene81957.t1	MTR_5g031230	23.496	349	228	11	37	379	39	354	6.71e-11	63.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene81957.t1	AT1G77120	84.085	377	60	0	3	379	2	378	0.0	673
MS.gene81957.t1	AT5G43940	58.445	373	155	0	4	376	3	375	2.70e-167	473
MS.gene81957.t1	AT5G43940	56.364	385	156	1	4	376	3	387	2.88e-163	463
MS.gene81957.t1	AT5G24760	51.882	372	175	2	7	378	12	379	4.99e-146	419
MS.gene81957.t1	AT1G64710	49.206	378	189	2	4	378	3	380	6.13e-141	406
MS.gene81957.t1	AT1G32780	49.231	390	188	2	1	380	1	390	7.03e-140	404
MS.gene81957.t1	AT5G24760	50.847	354	170	2	25	378	1	350	4.93e-135	390
MS.gene81957.t1	AT5G24760	50.847	354	170	2	25	378	1	350	4.93e-135	390
MS.gene81957.t1	AT1G64710	48.179	357	182	2	25	378	1	357	2.37e-129	375
MS.gene81957.t1	AT5G42250	46.257	374	198	3	9	379	17	390	5.67e-119	350
MS.gene81957.t1	AT1G22440	48.571	385	185	6	2	378	5	384	6.29e-117	345
MS.gene81957.t1	AT1G22430	46.457	381	201	3	1	378	6	386	1.00e-114	339
MS.gene81957.t1	AT1G22430	46.457	381	201	3	1	378	6	386	1.00e-114	339
MS.gene81957.t1	AT4G22110	46.457	381	195	4	6	380	12	389	1.80e-112	334
MS.gene81957.t1	AT4G22110	46.457	381	195	4	6	380	12	389	1.80e-112	334
MS.gene81957.t1	AT1G64710	47.059	306	160	1	75	378	6	311	5.90e-107	317
MS.gene81957.t1	AT5G24760	46.992	266	140	1	113	378	1	265	2.62e-91	275
MS.gene81957.t1	AT1G22430	49.650	286	141	3	1	283	6	291	1.57e-88	270
MS.gene81957.t1	AT1G22430	51.004	249	120	2	1	247	6	254	4.34e-80	248
MS.gene81957.t1	AT1G22430	51.004	249	120	2	1	247	6	254	4.34e-80	248
MS.gene81957.t1	AT4G22110	51.220	246	112	3	6	246	12	254	9.72e-76	236
MS.gene81957.t1	AT5G63620	30.749	374	235	14	12	372	57	419	1.62e-39	145
MS.gene81957.t1	AT5G63620	30.108	372	240	12	12	372	57	419	4.48e-39	144
MS.gene81957.t1	AT5G63620	30.400	250	159	8	12	251	57	301	6.35e-27	109
MS.gene81957.t1	AT5G51970	23.454	388	243	14	1	371	6	356	7.13e-18	84.7
MS.gene81957.t1	AT5G51970	23.454	388	243	14	1	371	6	356	7.13e-18	84.7
MS.gene81957.t1	AT1G72680	24.926	337	218	9	37	370	37	341	1.48e-12	68.6
MS.gene81957.t1	AT4G37990	24.679	312	210	9	37	346	35	323	4.59e-11	64.3

Find 118 sgRNAs with CRISPR-Local

Find 187 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CGTTCATCTGTTGCTATCTT+TGG	0.197894	3.2:+76305188	MS.gene81957:CDS
CAGGTTTGTCATGATCTTTC+GGG	0.232230	3.2:-76305571	None:intergenic
GCTTTGTTAATCTCTGAAAA+TGG	0.242441	3.2:-76306217	None:intergenic
GTTTCCAGCCGATATGAATT+AGG	0.257110	3.2:+76305410	MS.gene81957:CDS
GTCACACTGATGTTTACTTC+TGG	0.274790	3.2:+76304320	MS.gene81957:CDS
TTGTTGGCAGCTAAGAAGTT+TGG	0.287505	3.2:+76305530	MS.gene81957:intron
GCAACAGATGAACGGGGTTT+TGG	0.292075	3.2:-76305179	None:intergenic
CCATTGTTTCCTCGTATATT+TGG	0.294860	3.2:+76304562	MS.gene81957:CDS
ACAGGTTTGTCATGATCTTT+CGG	0.301321	3.2:-76305572	None:intergenic
TCAGTCAAGATTCTCTATTA+AGG	0.310729	3.2:+76304902	MS.gene81957:CDS
GCAACATGTGTGATCTTCTT+AGG	0.311495	3.2:+76304844	MS.gene81957:CDS
TGTTGGCAGCTAAGAAGTTT+GGG	0.311983	3.2:+76305531	MS.gene81957:intron
GCTGAAGGGGCAAGGATGTC+TGG	0.323064	3.2:+76305359	MS.gene81957:CDS
TGGGGTGTTGCTGTACTTGT+TGG	0.336473	3.2:+76305917	MS.gene81957:CDS
GTTGAATGAAAGGACTCTTA+AGG	0.347627	3.2:+76305985	MS.gene81957:CDS
CTTTCATTCAACAAATTCAT+AGG	0.348960	3.2:-76305974	None:intergenic
ATCTTTGGACTTGGAGCTGT+TGG	0.351212	3.2:+76305203	MS.gene81957:CDS
TTGAATGAAAGGACTCTTAA+GGG	0.375052	3.2:+76305986	MS.gene81957:CDS
GTTGAACTAACTTGTTGAAC+AGG	0.390252	3.2:-76305590	None:intergenic
ACTTTGTCAAGAGGTGCATC+AGG	0.391287	3.2:-76305008	None:intergenic
GCTGCGGTTGCATGGGAGGC+GGG	0.399452	3.2:+76304214	MS.gene81957:intron
TCACACTGATGTTTACTTCT+GGG	0.404127	3.2:+76304321	MS.gene81957:CDS
GGCGGGGAAGCCACTGGTGA+TGG	0.404743	3.2:+76304231	MS.gene81957:CDS
AAAGATAGCAACAGATGAAC+GGG	0.405118	3.2:-76305186	None:intergenic
TGGAACCAGCTGCGGTTGCA+TGG	0.416145	3.2:+76304206	MS.gene81957:intron
TGAAATGCATCGTCTTTGTT+TGG	0.418800	3.2:-76305944	None:intergenic
TCTGTTGCTATCTTTGGACT+TGG	0.419387	3.2:+76305194	MS.gene81957:CDS
GATATTACAGGAACTGGAAC+TGG	0.426601	3.2:+76306171	MS.gene81957:intron
CAGTCAAGATTCTCTATTAA+GGG	0.430904	3.2:+76304903	MS.gene81957:CDS
CGTATATTTGGTCATGAAGC+TGG	0.431276	3.2:+76304574	MS.gene81957:CDS
AGCTGCGGTTGCATGGGAGG+CGG	0.441799	3.2:+76304213	MS.gene81957:intron
ATCAGATGTATCATACGAAT+GGG	0.445545	3.2:+76306268	MS.gene81957:CDS
TTGTAGAAAAGTACATGAAA+GGG	0.453615	3.2:+76306053	MS.gene81957:CDS
TCTGGTGCATCAAGAATCAT+TGG	0.470218	3.2:+76305377	MS.gene81957:CDS
CTATGAATTTGTTGAATGAA+AGG	0.476135	3.2:+76305975	MS.gene81957:CDS
ACAGGCAGGGCATGATCCCC+TGG	0.483648	3.2:-76304774	None:intergenic
ACCAGCTGCGGTTGCATGGG+AGG	0.486050	3.2:+76304210	MS.gene81957:intron
CCACACTCTCCTGTGAATAC+AGG	0.486552	3.2:-76304792	None:intergenic
ATGGGAGGCGGGGAAGCCAC+TGG	0.486618	3.2:+76304225	MS.gene81957:CDS
TGTACCTAATTCATATCGGC+TGG	0.489612	3.2:-76305414	None:intergenic
ACCAAAATTTAGGCTGCTGA+AGG	0.497900	3.2:+76305344	MS.gene81957:intron
TTCTTAGGATCAACACGGAC+AGG	0.502318	3.2:+76304859	MS.gene81957:CDS
TTAACCATGTCCAACACTGC+TGG	0.504696	3.2:+76303827	None:intergenic
GGAACCAGCTGCGGTTGCAT+GGG	0.505012	3.2:+76304207	MS.gene81957:intron
GCATTGTACCTAATTCATAT+CGG	0.505878	3.2:-76305418	None:intergenic
GTTGTAGAAAAGTACATGAA+AGG	0.509192	3.2:+76306052	MS.gene81957:CDS
AACCTTAGGAAGATCAGTGC+GGG	0.515441	3.2:-76306032	None:intergenic
CCAAAATTTAGGCTGCTGAA+GGG	0.515926	3.2:+76305345	MS.gene81957:intron
ATGTAGATGTACCGACGAAA+TGG	0.519367	3.2:-76304938	None:intergenic
CATTCGTATGATACATCTGA+TGG	0.531716	3.2:-76306266	None:intergenic
CTGCGGTTGCATGGGAGGCG+GGG	0.533283	3.2:+76304215	MS.gene81957:CDS
GATAATTGTGATCAGGATTG+TGG	0.533379	3.2:+76304719	MS.gene81957:intron
CATGCCCTGCCTGTATTCAC+AGG	0.541877	3.2:+76304783	MS.gene81957:CDS
CTCAGCTGTGGAATATGCAC+AGG	0.542001	3.2:+76305038	MS.gene81957:CDS
TGTTTACTTCTGGGAAGCTA+AGG	0.543162	3.2:+76304330	MS.gene81957:CDS
AGACGGACTTCACCGGCTTG+TGG	0.546092	3.2:-76304274	None:intergenic
CATCAGATGTATCATACGAA+TGG	0.550987	3.2:+76306267	MS.gene81957:CDS
AGTAAACATCAGTGTGACAA+AGG	0.551878	3.2:-76304315	None:intergenic
CCTGTATTCACAGGAGAGTG+TGG	0.553209	3.2:+76304792	MS.gene81957:CDS
GTACCGACGAAATGGTGAAT+CGG	0.555313	3.2:-76304930	None:intergenic
AAAGTTTGTATTCTCAGCTG+TGG	0.560671	3.2:+76305026	MS.gene81957:CDS
CTGTATTCACAGGAGAGTGT+GGG	0.560874	3.2:+76304793	MS.gene81957:CDS
CAAAGATAGCAACAGATGAA+CGG	0.561211	3.2:-76305187	None:intergenic
GTTGGCAGCTAAGAAGTTTG+GGG	0.561606	3.2:+76305532	MS.gene81957:intron
TATCTTGAGACGGACTTCAC+CGG	0.564597	3.2:-76304281	None:intergenic
TGAATGTACCGGTAGCATCC+AGG	0.564756	3.2:+76305761	MS.gene81957:CDS
ACTCTCCTGTGAATACAGGC+AGG	0.564886	3.2:-76304788	None:intergenic
GATCATAGCCTGGATGCTAC+CGG	0.570554	3.2:-76305769	None:intergenic
GGTGTGACAGATCTAAAACC+AGG	0.572762	3.2:+76304756	MS.gene81957:CDS
GTAAACATCAGTGTGACAAA+GGG	0.577816	3.2:-76304314	None:intergenic
ACCCCGCACTGATCTTCCTA+AGG	0.578506	3.2:+76306030	MS.gene81957:CDS
CAAAATTTAGGCTGCTGAAG+GGG	0.579040	3.2:+76305346	MS.gene81957:intron
GAGTACACTGTGGTTCATGC+CGG	0.586769	3.2:+76304966	MS.gene81957:CDS
AGAATACAAACTTTGTCAAG+AGG	0.589584	3.2:-76305017	None:intergenic
TTTAGGCTGCTGAAGGGGCA+AGG	0.589857	3.2:+76305351	MS.gene81957:intron
CCAAATATACGAGGAAACAA+TGG	0.590862	3.2:-76304562	None:intergenic
GATTGTGGAGAGCGTAGGCG+AGG	0.594735	3.2:+76304734	MS.gene81957:CDS
TCTTAGGATCAACACGGACA+GGG	0.595918	3.2:+76304860	MS.gene81957:CDS
GAGGTGGCGCCACCACAAGC+CGG	0.602446	3.2:+76304262	MS.gene81957:CDS
GATGATCTGACCAGCAGTGT+TGG	0.604173	3.2:-76303837	None:intergenic
TGTAGAAAAGTACATGAAAG+GGG	0.605963	3.2:+76306054	MS.gene81957:CDS
CTTTGTCAAGAGGTGCATCA+GGG	0.608085	3.2:-76305007	None:intergenic
GTGTGACAGATCTAAAACCA+GGG	0.609191	3.2:+76304757	MS.gene81957:CDS
CGAATGCGGAGATCATAGCC+TGG	0.612238	3.2:-76305779	None:intergenic
ACTGGTGATGGAAGAAGTAG+AGG	0.612676	3.2:+76304243	MS.gene81957:CDS
ACTTGGAGCTGTTGGCCTTG+CGG	0.616948	3.2:+76305211	MS.gene81957:CDS
TCTACTTCTTCCATCACCAG+TGG	0.617716	3.2:-76304241	None:intergenic
CGGACTTCACCGGCTTGTGG+TGG	0.618100	3.2:-76304271	None:intergenic
CTGTCCACATTGTAAGTCAG+AGG	0.618115	3.2:+76304818	MS.gene81957:CDS
AGCACGATCCACACCTCCGT+TGG	0.619268	3.2:-76305736	None:intergenic
CAGCCGATTCACCATTTCGT+CGG	0.625858	3.2:+76304927	MS.gene81957:CDS
ATCGTGGACACATTCGAATG+CGG	0.633600	3.2:-76305793	None:intergenic
CTCTCCTCTGACTTACAATG+TGG	0.634714	3.2:-76304822	None:intergenic
TGTATTCACAGGAGAGTGTG+GGG	0.638812	3.2:+76304794	MS.gene81957:CDS
GATCGTGCTGTTGAATGTAC+CGG	0.639046	3.2:+76305750	MS.gene81957:CDS
ATATTTGGTCATGAAGCTGG+AGG	0.639817	3.2:+76304577	MS.gene81957:CDS
AACATCAGTGTGACAAAGGG+AGG	0.641785	3.2:-76304311	None:intergenic
AGGTGAAGAGTATCTTGAGA+CGG	0.642729	3.2:-76304291	None:intergenic
ATCAGGATTGTGGAGAGCGT+AGG	0.642832	3.2:+76304729	MS.gene81957:intron
CTCTCCTGTGAATACAGGCA+GGG	0.643982	3.2:-76304787	None:intergenic
TCAGATGTATCATACGAATG+GGG	0.645185	3.2:+76306269	MS.gene81957:CDS
GGTCAGATCATCAAGTGTAG+AGG	0.647505	3.2:+76303848	MS.gene81957:CDS
CAGATGTATCATACGAATGG+GGG	0.655213	3.2:+76306270	MS.gene81957:CDS
CTGACCAGCAGTGTTGGACA+TGG	0.657057	3.2:-76303831	None:intergenic
TATTTGGTCATGAAGCTGGA+GGG	0.661233	3.2:+76304578	MS.gene81957:CDS
CTTAGGATCAACACGGACAG+GGG	0.662424	3.2:+76304861	MS.gene81957:CDS
ATTGTGGAGAGCGTAGGCGA+GGG	0.662603	3.2:+76304735	MS.gene81957:CDS
GGTGATGGAAGAAGTAGAGG+TGG	0.673070	3.2:+76304246	MS.gene81957:CDS
TGAAATGACCAACGGAGGTG+TGG	0.677840	3.2:+76305728	MS.gene81957:CDS
GTAATTGCTGAAATGACCAA+CGG	0.682154	3.2:+76305720	MS.gene81957:CDS
ACCTTAGGAAGATCAGTGCG+GGG	0.690568	3.2:-76306031	None:intergenic
CAACCTTAGGAAGATCAGTG+CGG	0.707330	3.2:-76306033	None:intergenic
TGTGACAGATCTAAAACCAG+GGG	0.720106	3.2:+76304758	MS.gene81957:CDS
GCTTCATGACCAAATATACG+AGG	0.725898	3.2:-76304571	None:intergenic
TGATCTTCTTAGGATCAACA+CGG	0.743683	3.2:+76304854	MS.gene81957:CDS
ATTGCTGAAATGACCAACGG+AGG	0.744796	3.2:+76305723	MS.gene81957:CDS
TACATTCAGCGAGTACACTG+TGG	0.749330	3.2:+76304956	MS.gene81957:CDS
AAGATAGCAACAGATGAACG+GGG	0.767637	3.2:-76305185	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAAAAAAATAACAATCTAAA+TGG	-	chr3.2:76303941-76303960	None:intergenic	10.0%
!!	ACTTAAAATTAATTTATAGT+TGG	-	chr3.2:76303993-76304012	None:intergenic	10.0%
!!	ATATATTAATATCTAAACAT+TGG	-	chr3.2:76305075-76305094	None:intergenic	10.0%
!!!	TAATTTTAGTTACAAAATAT+TGG	+	chr3.2:76304431-76304450	MS.gene81957:intron	10.0%
!!	AGATATTAATATATGTTTCT+AGG	+	chr3.2:76305081-76305100	MS.gene81957:intron	15.0%
!!	ATTTATGTATAATTCTTCTT+AGG	+	chr3.2:76304406-76304425	MS.gene81957:intron	15.0%
!!	TTGTAAATTGCAATTATTAT+CGG	+	chr3.2:76305280-76305299	MS.gene81957:intron	15.0%
!!!	TTCTTTTTAAACCAAAATTT+AGG	+	chr3.2:76305334-76305353	MS.gene81957:intron	15.0%
!!	AAAATAACAATCTAAATGGA+AGG	-	chr3.2:76303937-76303956	None:intergenic	20.0%
!!	AGAATTTGAGAAAAAATAGA+TGG	-	chr3.2:76303876-76303895	None:intergenic	20.0%
!!	TCGAAAATTATTATCAATGT+CGG	+	chr3.2:76304107-76304126	MS.gene81957:intron	20.0%
!!	TGCAATATTAATTAATCTTC+CGG	-	chr3.2:76306111-76306130	None:intergenic	20.0%
!!!	TCTCTTATTTTTTTTCTTGT+TGG	+	chr3.2:76305514-76305533	MS.gene81957:intron	20.0%
!!!	TTGACTTTTTGTTTTTCTAA+GGG	+	chr3.2:76305893-76305912	MS.gene81957:intron	20.0%
!	CTTAGAAAAACAAAAAGTCA+AGG	-	chr3.2:76305894-76305913	None:intergenic	25.0%
!	CTTTCATTCAACAAATTCAT+AGG	-	chr3.2:76305977-76305996	None:intergenic	25.0%
!	TATGCTTTGATTACATAACA+CGG	+	chr3.2:76304353-76304372	MS.gene81957:intron	25.0%
!	TGATATTACTGTGATATTAC+AGG	+	chr3.2:76306159-76306178	MS.gene81957:intron	25.0%
!!	AACTTCAATCTTAAAGCATT+TGG	-	chr3.2:76305457-76305476	None:intergenic	25.0%
!!	GAATTTGAAGTATGATTCTA+TGG	+	chr3.2:76305639-76305658	MS.gene81957:intron	25.0%
!!	TCTTATATTCCTTTGTTTCA+GGG	+	chr3.2:76304532-76304551	MS.gene81957:intron	25.0%
!!	TGTTATTGATAATTGTGATC+AGG	+	chr3.2:76304712-76304731	MS.gene81957:intron	25.0%
!!	TTCTTATATTCCTTTGTTTC+AGG	+	chr3.2:76304531-76304550	MS.gene81957:intron	25.0%
!!	TTGTAGAAAAGTACATGAAA+GGG	+	chr3.2:76306053-76306072	MS.gene81957:CDS	25.0%
!!!	AATATTGGCATACTAGTTTT+TGG	+	chr3.2:76304446-76304465	MS.gene81957:intron	25.0%
!!!	ATTTTGTTTAACATACATCG+TGG	-	chr3.2:76305812-76305831	None:intergenic	25.0%
!!!	CTATGAATTTGTTGAATGAA+AGG	+	chr3.2:76305975-76305994	MS.gene81957:CDS	25.0%
!!!	CTTGACTTTTTGTTTTTCTA+AGG	+	chr3.2:76305892-76305911	MS.gene81957:intron	25.0%
!!!	TGATGTTTGATTTTTATACG+AGG	+	chr3.2:76305669-76305688	MS.gene81957:intron	25.0%
!!!	TTTTTGTTTTTCTAAGGGTT+GGG	+	chr3.2:76305898-76305917	MS.gene81957:intron	25.0%
	AAGTAGTATGATATGAAGCA+CGG	-	chr3.2:76304160-76304179	None:intergenic	30.0%
	AGAATACAAACTTTGTCAAG+AGG	-	chr3.2:76305020-76305039	None:intergenic	30.0%
	ATCAGATGTATCATACGAAT+GGG	+	chr3.2:76306268-76306287	MS.gene81957:CDS	30.0%
	CAGTCAAGATTCTCTATTAA+GGG	+	chr3.2:76304903-76304922	MS.gene81957:CDS	30.0%
	GAATTAGGAAAACATAGACT+CGG	-	chr3.2:76305107-76305126	None:intergenic	30.0%
	GCATTGTACCTAATTCATAT+CGG	-	chr3.2:76305421-76305440	None:intergenic	30.0%
	TCAGTCAAGATTCTCTATTA+AGG	+	chr3.2:76304902-76304921	MS.gene81957:CDS	30.0%
	TGCATTGCAGTGATTTAATA+TGG	+	chr3.2:76304506-76304525	MS.gene81957:intron	30.0%
	TTGAATGAAAGGACTCTTAA+GGG	+	chr3.2:76305986-76306005	MS.gene81957:CDS	30.0%
!	ATGTACTTTTCTACAACCTT+AGG	-	chr3.2:76306049-76306068	None:intergenic	30.0%
!	GCTTTGTTAATCTCTGAAAA+TGG	-	chr3.2:76306220-76306239	None:intergenic	30.0%
!	GTTGTAGAAAAGTACATGAA+AGG	+	chr3.2:76306052-76306071	MS.gene81957:CDS	30.0%
!	TGTAGAAAAGTACATGAAAG+GGG	+	chr3.2:76306054-76306073	MS.gene81957:CDS	30.0%
!	TTCAATCTTAAAGCATTTGG+TGG	-	chr3.2:76305454-76305473	None:intergenic	30.0%
!	TTTGGTTTAAAAAGAAGAGC+AGG	-	chr3.2:76305330-76305349	None:intergenic	30.0%
!!	ACTCTTAAGGGTACATTTTA+TGG	+	chr3.2:76305998-76306017	MS.gene81957:CDS	30.0%
!!	CTTTTGATTACATGCTGAAA+GGG	+	chr3.2:76306239-76306258	MS.gene81957:CDS	30.0%
!!	TTTTGATTACATGCTGAAAG+GGG	+	chr3.2:76306240-76306259	MS.gene81957:CDS	30.0%
!!!	CTTTTTGTTTTTCTAAGGGT+TGG	+	chr3.2:76305897-76305916	MS.gene81957:intron	30.0%
!!!	TTTCTAGCTTTTGCTTTTTC+AGG	+	chr3.2:76303909-76303928	MS.gene81957:intron	30.0%
!!!	TTTTGTTTTTCTAAGGGTTG+GGG	+	chr3.2:76305899-76305918	MS.gene81957:intron	30.0%
	AAAGATAGCAACAGATGAAC+GGG	-	chr3.2:76305189-76305208	None:intergenic	35.0%
	AAAGTTTGTATTCTCAGCTG+TGG	+	chr3.2:76305026-76305045	MS.gene81957:CDS	35.0%
	ACAGGTTTGTCATGATCTTT+CGG	-	chr3.2:76305575-76305594	None:intergenic	35.0%
	AGAAGAGCAGGAGTTAATAA+TGG	-	chr3.2:76305318-76305337	None:intergenic	35.0%
	AGTAAACATCAGTGTGACAA+AGG	-	chr3.2:76304318-76304337	None:intergenic	35.0%
	CAAAGATAGCAACAGATGAA+CGG	-	chr3.2:76305190-76305209	None:intergenic	35.0%
	CAGAAAATGCATAGAAATCG+TGG	-	chr3.2:76305615-76305634	None:intergenic	35.0%
	CATCAGATGTATCATACGAA+TGG	+	chr3.2:76306267-76306286	MS.gene81957:CDS	35.0%
	CATTCGTATGATACATCTGA+TGG	-	chr3.2:76306269-76306288	None:intergenic	35.0%
	CCAAATATACGAGGAAACAA+TGG	-	chr3.2:76304565-76304584	None:intergenic	35.0%
	CCATTGTTTCCTCGTATATT+TGG	+	chr3.2:76304562-76304581	MS.gene81957:CDS	35.0%
	GATAATTGTGATCAGGATTG+TGG	+	chr3.2:76304719-76304738	MS.gene81957:intron	35.0%
	GTAAACATCAGTGTGACAAA+GGG	-	chr3.2:76304317-76304336	None:intergenic	35.0%
	GTAATTGCTGAAATGACCAA+CGG	+	chr3.2:76305720-76305739	MS.gene81957:CDS	35.0%
	GTTGAATGAAAGGACTCTTA+AGG	+	chr3.2:76305985-76306004	MS.gene81957:CDS	35.0%
	TACTGTGATATTACAGGAAC+TGG	+	chr3.2:76306165-76306184	MS.gene81957:intron	35.0%
	TCAATGAAATCATATGCGTC+AGG	-	chr3.2:76304075-76304094	None:intergenic	35.0%
	TCACACTGATGTTTACTTCT+GGG	+	chr3.2:76304321-76304340	MS.gene81957:CDS	35.0%
	TCAGATGTATCATACGAATG+GGG	+	chr3.2:76306269-76306288	MS.gene81957:CDS	35.0%
	TGAAATGCATCGTCTTTGTT+TGG	-	chr3.2:76305947-76305966	None:intergenic	35.0%
	TGATCTTCTTAGGATCAACA+CGG	+	chr3.2:76304854-76304873	MS.gene81957:CDS	35.0%
	TGGTTCCAAAACACAATCAA+GGG	-	chr3.2:76304194-76304213	None:intergenic	35.0%
!	TGAATCCCTTGATTGTGTTT+TGG	+	chr3.2:76304186-76304205	MS.gene81957:intron	35.0%
!!	GCTTTTGATTACATGCTGAA+AGG	+	chr3.2:76306238-76306257	MS.gene81957:CDS	35.0%
!!	GGTTCTGTTTGTTTTCTTCA+AGG	+	chr3.2:76304467-76304486	MS.gene81957:intron	35.0%
!!	GTTGAACTAACTTGTTGAAC+AGG	-	chr3.2:76305593-76305612	None:intergenic	35.0%
!!	GTTGATTTTTGCAACACATC+CGG	-	chr3.2:76304988-76305007	None:intergenic	35.0%
!!	TCTTTTTAGCAACAAGCAAG+AGG	-	chr3.2:76305841-76305860	None:intergenic	35.0%
	AAGATAGCAACAGATGAACG+GGG	-	chr3.2:76305188-76305207	None:intergenic	40.0%
	ACCAAAATTTAGGCTGCTGA+AGG	+	chr3.2:76305344-76305363	MS.gene81957:intron	40.0%
	ATATTTGGTCATGAAGCTGG+AGG	+	chr3.2:76304577-76304596	MS.gene81957:CDS	40.0%
	ATCTGCTTCCATGCTTCTAT+AGG	+	chr3.2:76305128-76305147	MS.gene81957:intron	40.0%
	CAAAATTTAGGCTGCTGAAG+GGG	+	chr3.2:76305346-76305365	MS.gene81957:intron	40.0%
	CAGATGTATCATACGAATGG+GGG	+	chr3.2:76306270-76306289	MS.gene81957:CDS	40.0%
	CAGGTTTGTCATGATCTTTC+GGG	-	chr3.2:76305574-76305593	None:intergenic	40.0%
	CCAAAATTTAGGCTGCTGAA+GGG	+	chr3.2:76305345-76305364	MS.gene81957:intron	40.0%
	CGTATATTTGGTCATGAAGC+TGG	+	chr3.2:76304574-76304593	MS.gene81957:CDS	40.0%
	CGTTCATCTGTTGCTATCTT+TGG	+	chr3.2:76305188-76305207	MS.gene81957:CDS	40.0%
	CTGGTTCCAAAACACAATCA+AGG	-	chr3.2:76304195-76304214	None:intergenic	40.0%
	CTTTGATTACATAACACGGC+GGG	+	chr3.2:76304357-76304376	MS.gene81957:intron	40.0%
	GAAGTATGTTAGCGCTTCAT+AGG	-	chr3.2:76304031-76304050	None:intergenic	40.0%
	GATATTACAGGAACTGGAAC+TGG	+	chr3.2:76306171-76306190	MS.gene81957:intron	40.0%
	GCAACATGTGTGATCTTCTT+AGG	+	chr3.2:76304844-76304863	MS.gene81957:CDS	40.0%
	GCATGGAAGCAGATTGAATT+AGG	-	chr3.2:76305122-76305141	None:intergenic	40.0%
	GCGCTAACATACTTCAGATT+AGG	+	chr3.2:76304036-76304055	MS.gene81957:intron	40.0%
	GCTTCATGACCAAATATACG+AGG	-	chr3.2:76304574-76304593	None:intergenic	40.0%
	GCTTTGATTACATAACACGG+CGG	+	chr3.2:76304356-76304375	MS.gene81957:intron	40.0%
	GGAGATTGAGATGTGAACAT+TGG	+	chr3.2:76305690-76305709	MS.gene81957:intron	40.0%
	GGTTCAGCTTAGCTTAAGTA+AGG	-	chr3.2:76305873-76305892	None:intergenic	40.0%
	GTCACACTGATGTTTACTTC+TGG	+	chr3.2:76304320-76304339	MS.gene81957:CDS	40.0%
	GTTTCCAGCCGATATGAATT+AGG	+	chr3.2:76305410-76305429	MS.gene81957:CDS	40.0%
	TACAAGAGTAACATACCGCA+AGG	-	chr3.2:76305229-76305248	None:intergenic	40.0%
	TATTTGGTCATGAAGCTGGA+GGG	+	chr3.2:76304578-76304597	MS.gene81957:CDS	40.0%
	TCTGGTGCATCAAGAATCAT+TGG	+	chr3.2:76305377-76305396	MS.gene81957:CDS	40.0%
	TCTGTTGCTATCTTTGGACT+TGG	+	chr3.2:76305194-76305213	MS.gene81957:CDS	40.0%
	TGTACCTAATTCATATCGGC+TGG	-	chr3.2:76305417-76305436	None:intergenic	40.0%
	TGTTGGCAGCTAAGAAGTTT+GGG	+	chr3.2:76305531-76305550	MS.gene81957:intron	40.0%
	TTCCATGCTTCTATAGGTCT+TGG	+	chr3.2:76305134-76305153	MS.gene81957:intron	40.0%
	TTGTTGGCAGCTAAGAAGTT+TGG	+	chr3.2:76305530-76305549	MS.gene81957:intron	40.0%
!	ATGTAGATGTACCGACGAAA+TGG	-	chr3.2:76304941-76304960	None:intergenic	40.0%
!	GTGTGACAGATCTAAAACCA+GGG	+	chr3.2:76304757-76304776	MS.gene81957:CDS	40.0%
!	TGTGACAGATCTAAAACCAG+GGG	+	chr3.2:76304758-76304777	MS.gene81957:CDS	40.0%
!	TGTTTACTTCTGGGAAGCTA+AGG	+	chr3.2:76304330-76304349	MS.gene81957:CDS	40.0%
!!	AGATGTGAACATTGGTTTGC+AGG	+	chr3.2:76305698-76305717	MS.gene81957:intron	40.0%
!!	AGGTGAAGAGTATCTTGAGA+CGG	-	chr3.2:76304294-76304313	None:intergenic	40.0%
	AACATCAGTGTGACAAAGGG+AGG	-	chr3.2:76304314-76304333	None:intergenic	45.0%
	AACATGCGCATACACACTCA+AGG	-	chr3.2:76305252-76305271	None:intergenic	45.0%
	AACCTTAGGAAGATCAGTGC+GGG	-	chr3.2:76306035-76306054	None:intergenic	45.0%
	ATCGTGGACACATTCGAATG+CGG	-	chr3.2:76305796-76305815	None:intergenic	45.0%
	ATCTTTGGACTTGGAGCTGT+TGG	+	chr3.2:76305203-76305222	MS.gene81957:CDS	45.0%
	ATTGCTGAAATGACCAACGG+AGG	+	chr3.2:76305723-76305742	MS.gene81957:CDS	45.0%
	CAACCTTAGGAAGATCAGTG+CGG	-	chr3.2:76306036-76306055	None:intergenic	45.0%
	CACCAAGACCTATAGAAGCA+TGG	-	chr3.2:76305139-76305158	None:intergenic	45.0%
	CTCTCCTCTGACTTACAATG+TGG	-	chr3.2:76304825-76304844	None:intergenic	45.0%
	CTGTCCACATTGTAAGTCAG+AGG	+	chr3.2:76304818-76304837	MS.gene81957:CDS	45.0%
	GGTCAGATCATCAAGTGTAG+AGG	+	chr3.2:76303848-76303867	MS.gene81957:CDS	45.0%
	GTTGGCAGCTAAGAAGTTTG+GGG	+	chr3.2:76305532-76305551	MS.gene81957:intron	45.0%
	TACATTCAGCGAGTACACTG+TGG	+	chr3.2:76304956-76304975	MS.gene81957:CDS	45.0%
	TATGAAGCACGGATACACAC+CGG	-	chr3.2:76304149-76304168	None:intergenic	45.0%
	TCTACTTCTTCCATCACCAG+TGG	-	chr3.2:76304244-76304263	None:intergenic	45.0%
	TCTTAGGATCAACACGGACA+GGG	+	chr3.2:76304860-76304879	MS.gene81957:CDS	45.0%
	TTCTTAGGATCAACACGGAC+AGG	+	chr3.2:76304859-76304878	MS.gene81957:CDS	45.0%
!	ACTTTGTCAAGAGGTGCATC+AGG	-	chr3.2:76305011-76305030	None:intergenic	45.0%
!	CCCTTCAGCAGCCTAAATTT+TGG	-	chr3.2:76305348-76305367	None:intergenic	45.0%
!	CTGTATTCACAGGAGAGTGT+GGG	+	chr3.2:76304793-76304812	MS.gene81957:CDS	45.0%
!	CTTTGTCAAGAGGTGCATCA+GGG	-	chr3.2:76305010-76305029	None:intergenic	45.0%
!	GATCGTGCTGTTGAATGTAC+CGG	+	chr3.2:76305750-76305769	MS.gene81957:CDS	45.0%
!	GGTGTGACAGATCTAAAACC+AGG	+	chr3.2:76304756-76304775	MS.gene81957:CDS	45.0%
!	GTACCGACGAAATGGTGAAT+CGG	-	chr3.2:76304933-76304952	None:intergenic	45.0%
!	TATCTTGAGACGGACTTCAC+CGG	-	chr3.2:76304284-76304303	None:intergenic	45.0%
!	TGTATTCACAGGAGAGTGTG+GGG	+	chr3.2:76304794-76304813	MS.gene81957:CDS	45.0%
!!	ACTGGTGATGGAAGAAGTAG+AGG	+	chr3.2:76304243-76304262	MS.gene81957:CDS	45.0%
!!!	TTGTGTTTTGGAACCAGCTG+CGG	+	chr3.2:76304198-76304217	MS.gene81957:intron	45.0%
	ACCTTAGGAAGATCAGTGCG+GGG	-	chr3.2:76306034-76306053	None:intergenic	50.0%
	ACTCTCCTGTGAATACAGGC+AGG	-	chr3.2:76304791-76304810	None:intergenic	50.0%
	CATCGAATCGTGCACAGTTC+CGG	+	chr3.2:76306089-76306108	MS.gene81957:intron	50.0%
	CCACACTCTCCTGTGAATAC+AGG	-	chr3.2:76304795-76304814	None:intergenic	50.0%
	CCTGTATTCACAGGAGAGTG+TGG	+	chr3.2:76304792-76304811	MS.gene81957:CDS	50.0%
	CTCAGCTGTGGAATATGCAC+AGG	+	chr3.2:76305038-76305057	MS.gene81957:CDS	50.0%
	CTCTCCTGTGAATACAGGCA+GGG	-	chr3.2:76304790-76304809	None:intergenic	50.0%
	CTTAGGATCAACACGGACAG+GGG	+	chr3.2:76304861-76304880	MS.gene81957:CDS	50.0%
	GAGTACACTGTGGTTCATGC+CGG	+	chr3.2:76304966-76304985	MS.gene81957:CDS	50.0%
	GATACACACCGGACACAACA+TGG	-	chr3.2:76304138-76304157	None:intergenic	50.0%
	GATCATAGCCTGGATGCTAC+CGG	-	chr3.2:76305772-76305791	None:intergenic	50.0%
	GATGATCTGACCAGCAGTGT+TGG	-	chr3.2:76303840-76303859	None:intergenic	50.0%
	GGAGTCTGACCCTGAAACAA+AGG	-	chr3.2:76304544-76304563	None:intergenic	50.0%
	TGAAATGACCAACGGAGGTG+TGG	+	chr3.2:76305728-76305747	MS.gene81957:CDS	50.0%
!	ATCAGGATTGTGGAGAGCGT+AGG	+	chr3.2:76304729-76304748	MS.gene81957:intron	50.0%
!	CAGCCGATTCACCATTTCGT+CGG	+	chr3.2:76304927-76304946	MS.gene81957:CDS	50.0%
!	GCAACAGATGAACGGGGTTT+TGG	-	chr3.2:76305182-76305201	None:intergenic	50.0%
!	TGAATGTACCGGTAGCATCC+AGG	+	chr3.2:76305761-76305780	MS.gene81957:CDS	50.0%
!!	GGTGATGGAAGAAGTAGAGG+TGG	+	chr3.2:76304246-76304265	MS.gene81957:CDS	50.0%
!!	TGGGGTGTTGCTGTACTTGT+TGG	+	chr3.2:76305917-76305936	MS.gene81957:CDS	50.0%
	CATGCCCTGCCTGTATTCAC+AGG	+	chr3.2:76304783-76304802	MS.gene81957:CDS	55.0%
	CGAATGCGGAGATCATAGCC+TGG	-	chr3.2:76305782-76305801	None:intergenic	55.0%
	CGGTGTGTCCATGTTGTGTC+CGG	+	chr3.2:76304127-76304146	MS.gene81957:intron	55.0%
	TTTAGGCTGCTGAAGGGGCA+AGG	+	chr3.2:76305351-76305370	MS.gene81957:intron	55.0%
!	ACCCCGCACTGATCTTCCTA+AGG	+	chr3.2:76306030-76306049	MS.gene81957:CDS	55.0%
!	ATTGTGGAGAGCGTAGGCGA+GGG	+	chr3.2:76304735-76304754	MS.gene81957:CDS	55.0%
!!	ACTTGGAGCTGTTGGCCTTG+CGG	+	chr3.2:76305211-76305230	MS.gene81957:CDS	55.0%
	AGCACGATCCACACCTCCGT+TGG	-	chr3.2:76305739-76305758	None:intergenic	60.0%
	GCTGAAGGGGCAAGGATGTC+TGG	+	chr3.2:76305359-76305378	MS.gene81957:CDS	60.0%
	GGAACCAGCTGCGGTTGCAT+GGG	+	chr3.2:76304207-76304226	MS.gene81957:intron	60.0%
	TGGAACCAGCTGCGGTTGCA+TGG	+	chr3.2:76304206-76304225	MS.gene81957:intron	60.0%
!	GATTGTGGAGAGCGTAGGCG+AGG	+	chr3.2:76304734-76304753	MS.gene81957:CDS	60.0%
!!	AGACGGACTTCACCGGCTTG+TGG	-	chr3.2:76304277-76304296	None:intergenic	60.0%
	ACAGGCAGGGCATGATCCCC+TGG	-	chr3.2:76304777-76304796	None:intergenic	65.0%
	ACCAGCTGCGGTTGCATGGG+AGG	+	chr3.2:76304210-76304229	MS.gene81957:intron	65.0%
	AGCTGCGGTTGCATGGGAGG+CGG	+	chr3.2:76304213-76304232	MS.gene81957:intron	65.0%
!!	CGGACTTCACCGGCTTGTGG+TGG	-	chr3.2:76304274-76304293	None:intergenic	65.0%
	ATGGGAGGCGGGGAAGCCAC+TGG	+	chr3.2:76304225-76304244	MS.gene81957:CDS	70.0%
	CTGCGGTTGCATGGGAGGCG+GGG	+	chr3.2:76304215-76304234	MS.gene81957:CDS	70.0%
	GAGGTGGCGCCACCACAAGC+CGG	+	chr3.2:76304262-76304281	MS.gene81957:CDS	70.0%
	GCCTCCCATGCAACCGCAGC+TGG	-	chr3.2:76304214-76304233	None:intergenic	70.0%
	GCTGCGGTTGCATGGGAGGC+GGG	+	chr3.2:76304214-76304233	MS.gene81957:intron	70.0%
	GGCGGGGAAGCCACTGGTGA+TGG	+	chr3.2:76304231-76304250	MS.gene81957:CDS	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.2	gene	76303833	76306297	76303833	ID=MS.gene81957
chr3.2	mRNA	76303833	76306297	76303833	ID=MS.gene81957.t1;Parent=MS.gene81957
chr3.2	exon	76303833	76303869	76303833	ID=MS.gene81957.t1.exon1;Parent=MS.gene81957.t1
chr3.2	CDS	76303833	76303869	76303833	ID=cds.MS.gene81957.t1;Parent=MS.gene81957.t1
chr3.2	exon	76304215	76304351	76304215	ID=MS.gene81957.t1.exon2;Parent=MS.gene81957.t1
chr3.2	CDS	76304215	76304351	76304215	ID=cds.MS.gene81957.t1;Parent=MS.gene81957.t1
chr3.2	exon	76304553	76304599	76304553	ID=MS.gene81957.t1.exon3;Parent=MS.gene81957.t1
chr3.2	CDS	76304553	76304599	76304553	ID=cds.MS.gene81957.t1;Parent=MS.gene81957.t1
chr3.2	exon	76304734	76305059	76304734	ID=MS.gene81957.t1.exon4;Parent=MS.gene81957.t1
chr3.2	CDS	76304734	76305059	76304734	ID=cds.MS.gene81957.t1;Parent=MS.gene81957.t1
chr3.2	exon	76305150	76305232	76305150	ID=MS.gene81957.t1.exon5;Parent=MS.gene81957.t1
chr3.2	CDS	76305150	76305232	76305150	ID=cds.MS.gene81957.t1;Parent=MS.gene81957.t1
chr3.2	exon	76305356	76305431	76305356	ID=MS.gene81957.t1.exon6;Parent=MS.gene81957.t1
chr3.2	CDS	76305356	76305431	76305356	ID=cds.MS.gene81957.t1;Parent=MS.gene81957.t1
chr3.2	exon	76305540	76305601	76305540	ID=MS.gene81957.t1.exon7;Parent=MS.gene81957.t1
chr3.2	CDS	76305540	76305601	76305540	ID=cds.MS.gene81957.t1;Parent=MS.gene81957.t1
chr3.2	exon	76305720	76305815	76305720	ID=MS.gene81957.t1.exon8;Parent=MS.gene81957.t1
chr3.2	CDS	76305720	76305815	76305720	ID=cds.MS.gene81957.t1;Parent=MS.gene81957.t1
chr3.2	exon	76305914	76306075	76305914	ID=MS.gene81957.t1.exon9;Parent=MS.gene81957.t1
chr3.2	CDS	76305914	76306075	76305914	ID=cds.MS.gene81957.t1;Parent=MS.gene81957.t1
chr3.2	exon	76306181	76306297	76306181	ID=MS.gene81957.t1.exon10;Parent=MS.gene81957.t1
chr3.2	CDS	76306181	76306297	76306181	ID=cds.MS.gene81957.t1;Parent=MS.gene81957.t1

Gene Sequence

>MS.gene81957

ATGTCCAACACTGCTGGTCAGATCATCAAGTGTAGAGGTACCATCTATTTTTTCTCAAATTCTGTTTTGATCTTGTTTTCTAGCTTTTGCTTTTTCAGGATCCTTCCATTTAGATTGTTATTTTTTTTTTATTAATCATTTCATTTTATTTTATTTGCCAACTATAAATTAATTTTAAGTGCTACATACATACTACCTATGAAGCGCTAACATACTTCAGATTAGGCGTGTCTATGACACCTGACGCATATGATTTCATTGAATTATGTGATTTTCGAAAATTATTATCAATGTCGGTGTGTCCATGTTGTGTCCGGTGTGTATCCGTGCTTCATATCATACTACTTACTAACTGAATCCCTTGATTGTGTTTTGGAACCAGCTGCGGTTGCATGGGAGGCGGGGAAGCCACTGGTGATGGAAGAAGTAGAGGTGGCGCCACCACAAGCCGGTGAAGTCCGTCTCAAGATACTCTTCACCTCCCTTTGTCACACTGATGTTTACTTCTGGGAAGCTAAGGTATGCTTTGATTACATAACACGGCGGGTGCTTAGTTTTCTTACACAAAAGTTAATTTATGTATAATTCTTCTTAGGTTTAATTTTAGTTACAAAATATTGGCATACTAGTTTTTGGTTCTGTTTGTTTTCTTCAAGGTTTTGATGTTCTGTTTTGCATTGCAGTGATTTAATATGGTTTTCTTATATTCCTTTGTTTCAGGGTCAGACTCCATTGTTTCCTCGTATATTTGGTCATGAAGCTGGAGGGTATGACTCTCTTCTGAACATTGAAAGTTGAATCACATATTATAAATCTTTCTCTTATATATTGTTGATTAAATCTGTTTAGCTATCTAATTTGTTATGAAATGTGAATGAATGTTATTGATAATTGTGATCAGGATTGTGGAGAGCGTAGGCGAGGGTGTGACAGATCTAAAACCAGGGGATCATGCCCTGCCTGTATTCACAGGAGAGTGTGGGGACTGTCCACATTGTAAGTCAGAGGAGAGCAACATGTGTGATCTTCTTAGGATCAACACGGACAGGGGCGTTATGATCAATGACAATCAGTCAAGATTCTCTATTAAGGGACAGCCGATTCACCATTTCGTCGGTACATCTACATTCAGCGAGTACACTGTGGTTCATGCCGGATGTGTTGCAAAAATCAACCCTGATGCACCTCTTGACAAAGTTTGTATTCTCAGCTGTGGAATATGCACAGGTATAGTAGATTCCAATGTTTAGATATTAATATATGTTTCTAGGCCGAGTCTATGTTTTCCTAATTCAATCTGCTTCCATGCTTCTATAGGTCTTGGTGCTACTATCAATGTTGCTAAACCAAAACCCCGTTCATCTGTTGCTATCTTTGGACTTGGAGCTGTTGGCCTTGCGGTATGTTACTCTTGTACCTTGAGTGTGTATGCGCATGTTTGTGTGTGTTGTAAATTGCAATTATTATCGGAAATGAAAAGAGCCATTATTAACTCCTGCTCTTCTTTTTAAACCAAAATTTAGGCTGCTGAAGGGGCAAGGATGTCTGGTGCATCAAGAATCATTGGAGTTGATTTAGTTTCCAGCCGATATGAATTAGGTACAATGCTTTTCTTATTCCACCAAATGCTTTAAGATTGAAGTTAACACAAATTCAATGACTGTTTCTTTTATTGATGACATCTCTTATTTTTTTTCTTGTTGGCAGCTAAGAAGTTTGGGGTAAATGAGTTCGTAAACCCGAAAGATCATGACAAACCTGTTCAACAAGTTAGTTCAACCACGATTTCTATGCATTTTCTGTTGTGAATTTGAAGTATGATTCTATGGATTAAAATGATGTTTGATTTTTATACGAGGAGATTGAGATGTGAACATTGGTTTGCAGGTAATTGCTGAAATGACCAACGGAGGTGTGGATCGTGCTGTTGAATGTACCGGTAGCATCCAGGCTATGATCTCCGCATTCGAATGTGTCCACGATGTATGTTAAACAAAATTCACTTCCTCTTGCTTGTTGCTAAAAAGAAATTATAAACCTTACTTAAGCTAAGCTGAACCTTGACTTTTTGTTTTTCTAAGGGTTGGGGTGTTGCTGTACTTGTTGGAGTGCCAAACAAAGACGATGCATTTCAAACACATCCTATGAATTTGTTGAATGAAAGGACTCTTAAGGGTACATTTTATGGAAACTACAAACCCCGCACTGATCTTCCTAAGGTTGTAGAAAAGTACATGAAAGGGGTAAGTTTATATACATCGAATCGTGCACAGTTCCGGAAGATTAATTAATATTGCAGAATGATATGTTATTTGTATTGCTAATATGATATTACTGTGATATTACAGGAACTGGAACTGGAGAAATTCATTACTCACACAATTCCATTTTCAGAGATTAACAAAGCTTTTGATTACATGCTGAAAGGGGAGTCCATCAGATGTATCATACGAATGGGGGAGTAA

Protein sequence

>MS.gene81957.t1

MSNTAGQIIKCRAAVAWEAGKPLVMEEVEVAPPQAGEVRLKILFTSLCHTDVYFWEAKGQTPLFPRIFGHEAGGIVESVGEGVTDLKPGDHALPVFTGECGDCPHCKSEESNMCDLLRINTDRGVMINDNQSRFSIKGQPIHHFVGTSTFSEYTVVHAGCVAKINPDAPLDKVCILSCGICTGLGATINVAKPKPRSSVAIFGLGAVGLAAAEGARMSGASRIIGVDLVSSRYELAKKFGVNEFVNPKDHDKPVQQVIAEMTNGGVDRAVECTGSIQAMISAFECVHDGWGVAVLVGVPNKDDAFQTHPMNLLNERTLKGTFYGNYKPRTDLPKVVEKYMKGELELEKFITHTIPFSEINKAFDYMLKGESIRCIIRMGE