AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene058174


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene058174.t1 MTR_2g035190 95.513 156 7 0 1 156 1 156 1.68e-105 299
MS.gene058174.t1 MTR_2g035320 94.231 156 9 0 1 156 1 156 3.38e-105 298
MS.gene058174.t1 MTR_2g035210 89.744 156 16 0 1 156 1 156 7.25e-100 285
MS.gene058174.t1 MTR_2g035220 88.462 156 18 0 1 156 1 156 5.11e-97 277
MS.gene058174.t1 MTR_6g033450 66.667 156 52 0 1 156 1 156 3.17e-69 207
MS.gene058174.t1 MTR_4g120970 66.667 156 52 0 1 156 1 156 3.17e-69 207
MS.gene058174.t1 MTR_2g035100 62.179 156 59 0 1 156 1 156 4.03e-66 199
MS.gene058174.t1 MTR_2g035105 61.538 156 60 0 1 156 1 156 3.75e-65 197
MS.gene058174.t1 MTR_2g035150 62.179 156 59 0 1 156 1 156 2.02e-64 195
MS.gene058174.t1 MTR_2g035130 60.256 156 62 0 1 156 1 156 9.36e-64 193
MS.gene058174.t1 MTR_2g035120 57.692 156 66 0 1 156 1 156 5.58e-60 184
MS.gene058174.t1 MTR_2g035320 88.776 98 11 0 59 156 24 121 2.87e-59 181
MS.gene058174.t1 MTR_2g035170 47.436 156 82 0 1 156 1 156 1.16e-47 152
MS.gene058174.t1 MTR_4g120950 41.104 163 89 5 1 159 1 160 1.19e-30 109
MS.gene058174.t1 MTR_4g120760 33.121 157 104 1 1 156 1 157 8.32e-29 104
MS.gene058174.t1 MTR_4g120760 33.121 157 104 1 1 156 1 157 8.69e-29 104
MS.gene058174.t1 MTR_2g435310 31.410 156 106 1 1 155 1 156 1.31e-28 104
MS.gene058174.t1 MTR_4g120940 62.500 72 27 0 85 156 47 118 1.73e-22 87.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 46 sgRNAs with CRISPR-Local

Find 68 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTAAACATTTGCATTAATT+TGG 0.169383 2.1:-57673664 MS.gene058174:CDS
ATGTTTAATTTGCTAGAATT+AGG 0.204550 2.1:+57673678 None:intergenic
GGATAGCTTCTTAATAGTTC+CGG 0.311830 2.1:+57674566 None:intergenic
TACAGCTTAGTGGGAGGAAC+AGG 0.324066 2.1:-57673894 MS.gene058174:CDS
CTTGATAAGTCCAGTTCCTT+TGG 0.329858 2.1:+57673728 None:intergenic
TGTTTAATTTGCTAGAATTA+GGG 0.341815 2.1:+57673679 None:intergenic
TTCAACAATTTCAACACTTT+GGG 0.350751 2.1:+57674602 None:intergenic
CTTCAACAATTTCAACACTT+TGG 0.364915 2.1:+57674601 None:intergenic
TGGGCAGCAGAAATCACCTT+TGG 0.387864 2.1:+57674621 None:intergenic
AGATAGAGTTGCATCACCTT+TGG 0.394288 2.1:+57673776 None:intergenic
GATAGCTTCTTAATAGTTCC+GGG 0.397748 2.1:+57674567 None:intergenic
GTTGAAATTGTTGAAGGAAA+TGG 0.402462 2.1:-57674594 MS.gene058174:CDS
GAGACTAAGGCCAAAGGAAC+TGG 0.415366 2.1:-57673738 MS.gene058174:CDS
AGAGCCTTGTAGAGTTTAGC+TGG 0.426244 2.1:+57674669 None:intergenic
GGGCAGCAGAAATCACCTTT+GGG 0.449440 2.1:+57674622 None:intergenic
TGGATATAACTACAGCTTAG+TGG 0.476164 2.1:-57673904 MS.gene058174:CDS
AAAATTGTTGCTGGCTCTGA+TGG 0.476544 2.1:-57673834 MS.gene058174:CDS
GCAGTGGATGAGGCAAACTT+TGG 0.494243 2.1:-57673924 MS.gene058174:CDS
CAAATTAGATGCAGTGGATG+AGG 0.499478 2.1:-57673934 MS.gene058174:CDS
TAATTTGTGTAGCACAAAGT+TGG 0.501990 2.1:+57673950 None:intergenic
CAAAGTGTTGAAATTGTTGA+AGG 0.502542 2.1:-57674600 MS.gene058174:CDS
GGATATAACTACAGCTTAGT+GGG 0.506709 2.1:-57673903 MS.gene058174:CDS
ATCTGAATTACCTTCAACAA+TGG 0.509250 2.1:+57674545 None:intergenic
GGACTTATCAAGGCCATTGA+GGG 0.514208 2.1:-57673717 MS.gene058174:CDS
CAAAGGAACTGGACTTATCA+AGG 0.516806 2.1:-57673727 MS.gene058174:CDS
AAGAAGCTATCCATTGTTGA+AGG 0.523757 2.1:-57674555 MS.gene058174:intron
AGCAGTACGTGAGGAGACTA+AGG 0.536209 2.1:-57673751 MS.gene058174:CDS
GAGTTGCATCACCTTTGGTA+TGG 0.537356 2.1:+57673781 None:intergenic
TGCCACGACGTAGCCCTCAA+TGG 0.549026 2.1:+57673704 None:intergenic
GGCTCCAGCTAAACTCTACA+AGG 0.556705 2.1:-57674673 MS.gene058174:CDS
ATTGTTGCTGGCTCTGATGG+TGG 0.570543 2.1:-57673831 MS.gene058174:CDS
TGGACTTATCAAGGCCATTG+AGG 0.572485 2.1:-57673718 MS.gene058174:CDS
GTTGAAGGAAATGGAGGACC+CGG 0.599694 2.1:-57674585 MS.gene058174:CDS
GTAGAGTTTAGCTGGAGCCA+CGG 0.602676 2.1:+57674677 None:intergenic
TGCTGATGAAATCGTCCCAA+AGG 0.606260 2.1:-57674637 MS.gene058174:CDS
CTTAGTGGGAGGAACAGGGT+TGG 0.609437 2.1:-57673889 MS.gene058174:CDS
GAAATTGTTGAAGGAAATGG+AGG 0.626795 2.1:-57674591 MS.gene058174:CDS
GCTACACAAATTAGATGCAG+TGG 0.633814 2.1:-57673940 MS.gene058174:CDS
TTTCACTGAAATCTTAACAA+CGG 0.637000 2.1:+57673806 None:intergenic
GGCCATTGAGGGCTACGTCG+TGG 0.646344 2.1:-57673706 MS.gene058174:CDS
TCAGTGAAATACCATACCAA+AGG 0.652605 2.1:-57673792 MS.gene058174:CDS
CGTGAGGAGACTAAGGCCAA+AGG 0.670663 2.1:-57673744 MS.gene058174:CDS
TCTATCTGAAGCAGTACGTG+AGG 0.676642 2.1:-57673760 MS.gene058174:CDS
ACAGCTTAGTGGGAGGAACA+GGG 0.680453 2.1:-57673893 MS.gene058174:CDS
TATAACTACAGCTTAGTGGG+AGG 0.697586 2.1:-57673900 MS.gene058174:CDS
TGACGAACATGTCTCAACCG+TGG 0.725590 2.1:-57674694 MS.gene058174:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATTATATAAATAATTATAA+TGG - chr2.1:57674362-57674381 MS.gene058174:intron 0.0%
!!! AAATAAAAGTATAACATAAA+TGG + chr2.1:57674167-57674186 None:intergenic 10.0%
!!! TTTGTCAATTTTTAAATAAA+AGG + chr2.1:57673954-57673973 None:intergenic 10.0%
!!! TTTTATACCAAAAAAAAAAA+AGG - chr2.1:57674080-57674099 MS.gene058174:intron 10.0%
!! ATTAAACATTTGCATTAATT+TGG - chr2.1:57674691-57674710 MS.gene058174:CDS 15.0%
!!! TAATTTAACTTTCTTTTCTT+TGG + chr2.1:57674059-57674078 None:intergenic 15.0%
!!! TTAACTTCCTTTTTTTTTTT+TGG + chr2.1:57674090-57674109 None:intergenic 15.0%
!! AATAGTAATCCAATTCTATT+TGG - chr2.1:57674129-57674148 MS.gene058174:intron 20.0%
!!! ATGTTTAATTTGCTAGAATT+AGG + chr2.1:57674680-57674699 None:intergenic 20.0%
!!! TGTTTAATTTGCTAGAATTA+GGG + chr2.1:57674679-57674698 None:intergenic 20.0%
! AATAGAATCTTGAGAAATGA+AGG - chr2.1:57674190-57674209 MS.gene058174:intron 25.0%
! AGGGTTATGTCTAATAATTA+TGG - chr2.1:57674234-57674253 MS.gene058174:intron 25.0%
! ATAATTATTAGACATAACCC+TGG + chr2.1:57674235-57674254 None:intergenic 25.0%
! TATAATGGTTATATTGCAGA+TGG - chr2.1:57674377-57674396 MS.gene058174:intron 25.0%
! TTCAACAATTTCAACACTTT+GGG + chr2.1:57673756-57673775 None:intergenic 25.0%
! TTCAGATGATTATTGATACT+CGG - chr2.1:57674273-57674292 MS.gene058174:intron 25.0%
! TTTCACTGAAATCTTAACAA+CGG + chr2.1:57674552-57674571 None:intergenic 25.0%
!!! CATCTGAATCATTTTTTTGA+AGG + chr2.1:57674261-57674280 None:intergenic 25.0%
ATCTGAATTACCTTCAACAA+TGG + chr2.1:57673813-57673832 None:intergenic 30.0%
CATGACTATCCAAATAGAAT+TGG + chr2.1:57674141-57674160 None:intergenic 30.0%
CTTCAACAATTTCAACACTT+TGG + chr2.1:57673757-57673776 None:intergenic 30.0%
GTTGAAATTGTTGAAGGAAA+TGG - chr2.1:57673761-57673780 MS.gene058174:CDS 30.0%
! TAATTTGTGTAGCACAAAGT+TGG + chr2.1:57674408-57674427 None:intergenic 30.0%
! TTTGAGACAAAAATTGTTGC+TGG - chr2.1:57674512-57674531 MS.gene058174:intron 30.0%
!! CAAAGTGTTGAAATTGTTGA+AGG - chr2.1:57673755-57673774 MS.gene058174:CDS 30.0%
AAGAAGCTATCCATTGTTGA+AGG - chr2.1:57673800-57673819 MS.gene058174:CDS 35.0%
ATAATACATAGCTCGTTCCA+GGG - chr2.1:57674215-57674234 MS.gene058174:intron 35.0%
GAAATTGTTGAAGGAAATGG+AGG - chr2.1:57673764-57673783 MS.gene058174:CDS 35.0%
GACAAACATGATGTGACAAA+TGG - chr2.1:57673968-57673987 MS.gene058174:intron 35.0%
GATAGCTTCTTAATAGTTCC+GGG + chr2.1:57673791-57673810 None:intergenic 35.0%
GGATAGCTTCTTAATAGTTC+CGG + chr2.1:57673792-57673811 None:intergenic 35.0%
GGATATAACTACAGCTTAGT+GGG - chr2.1:57674452-57674471 MS.gene058174:intron 35.0%
TCAGTGAAATACCATACCAA+AGG - chr2.1:57674563-57674582 MS.gene058174:CDS 35.0%
TGGATATAACTACAGCTTAG+TGG - chr2.1:57674451-57674470 MS.gene058174:intron 35.0%
! GTTGTACCGACAAATTTGAT+CGG + chr2.1:57673914-57673933 None:intergenic 35.0%
!! CGGTCTAAAACAATTTTACG+CGG + chr2.1:57673887-57673906 None:intergenic 35.0%
AGATAGAGTTGCATCACCTT+TGG + chr2.1:57674582-57674601 None:intergenic 40.0%
CAAAGGAACTGGACTTATCA+AGG - chr2.1:57674628-57674647 MS.gene058174:CDS 40.0%
CAAATTAGATGCAGTGGATG+AGG - chr2.1:57674421-57674440 MS.gene058174:intron 40.0%
CGACAAATTTGATCGGTTGT+CGG + chr2.1:57673907-57673926 None:intergenic 40.0%
CGACAACCGATCAAATTTGT+CGG - chr2.1:57673905-57673924 MS.gene058174:CDS 40.0%
CTTGATAAGTCCAGTTCCTT+TGG + chr2.1:57674630-57674649 None:intergenic 40.0%
GATAATACATAGCTCGTTCC+AGG - chr2.1:57674214-57674233 MS.gene058174:intron 40.0%
GCTACACAAATTAGATGCAG+TGG - chr2.1:57674415-57674434 MS.gene058174:intron 40.0%
TATAACTACAGCTTAGTGGG+AGG - chr2.1:57674455-57674474 MS.gene058174:intron 40.0%
!! AAAATTGTTGCTGGCTCTGA+TGG - chr2.1:57674521-57674540 MS.gene058174:intron 40.0%
GAGTTGCATCACCTTTGGTA+TGG + chr2.1:57674577-57674596 None:intergenic 45.0%
GGACTTATCAAGGCCATTGA+GGG - chr2.1:57674638-57674657 MS.gene058174:CDS 45.0%
TGCTGATGAAATCGTCCCAA+AGG - chr2.1:57673718-57673737 MS.gene058174:CDS 45.0%
TGGACTTATCAAGGCCATTG+AGG - chr2.1:57674637-57674656 MS.gene058174:CDS 45.0%
! AGAGCCTTGTAGAGTTTAGC+TGG + chr2.1:57673689-57673708 None:intergenic 45.0%
! TCTATCTGAAGCAGTACGTG+AGG - chr2.1:57674595-57674614 MS.gene058174:CDS 45.0%
ACAGCTTAGTGGGAGGAACA+GGG - chr2.1:57674462-57674481 MS.gene058174:intron 50.0%
AGCAGTACGTGAGGAGACTA+AGG - chr2.1:57674604-57674623 MS.gene058174:CDS 50.0%
GAGACTAAGGCCAAAGGAAC+TGG - chr2.1:57674617-57674636 MS.gene058174:CDS 50.0%
GCAGTGGATGAGGCAAACTT+TGG - chr2.1:57674431-57674450 MS.gene058174:intron 50.0%
GGCTCCAGCTAAACTCTACA+AGG - chr2.1:57673682-57673701 MS.gene058174:CDS 50.0%
GGGCAGCAGAAATCACCTTT+GGG + chr2.1:57673736-57673755 None:intergenic 50.0%
GTAGAGTTTAGCTGGAGCCA+CGG + chr2.1:57673681-57673700 None:intergenic 50.0%
GTTGAAGGAAATGGAGGACC+CGG - chr2.1:57673770-57673789 MS.gene058174:CDS 50.0%
TACAGCTTAGTGGGAGGAAC+AGG - chr2.1:57674461-57674480 MS.gene058174:intron 50.0%
TGACGAACATGTCTCAACCG+TGG - chr2.1:57673661-57673680 MS.gene058174:CDS 50.0%
TGGGCAGCAGAAATCACCTT+TGG + chr2.1:57673737-57673756 None:intergenic 50.0%
!! ATTGTTGCTGGCTCTGATGG+TGG - chr2.1:57674524-57674543 MS.gene058174:intron 50.0%
CGTGAGGAGACTAAGGCCAA+AGG - chr2.1:57674611-57674630 MS.gene058174:CDS 55.0%
CTTAGTGGGAGGAACAGGGT+TGG - chr2.1:57674466-57674485 MS.gene058174:intron 55.0%
TGCCACGACGTAGCCCTCAA+TGG + chr2.1:57674654-57674673 None:intergenic 60.0%
GGCCATTGAGGGCTACGTCG+TGG - chr2.1:57674649-57674668 MS.gene058174:CDS 65.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 57673641 57674736 57673641 ID=MS.gene058174
chr2.1 mRNA 57673641 57674736 57673641 ID=MS.gene058174.t1;Parent=MS.gene058174
chr2.1 exon 57674556 57674736 57674556 ID=MS.gene058174.t1.exon1;Parent=MS.gene058174.t1
chr2.1 CDS 57674556 57674736 57674556 ID=cds.MS.gene058174.t1;Parent=MS.gene058174.t1
chr2.1 exon 57673641 57673981 57673641 ID=MS.gene058174.t1.exon2;Parent=MS.gene058174.t1
chr2.1 CDS 57673641 57673981 57673641 ID=cds.MS.gene058174.t1;Parent=MS.gene058174.t1
Gene Sequence

>MS.gene058174

ATGGGTGTTTTTACTTTCAATGACGAACATGTCTCAACCGTGGCTCCAGCTAAACTCTACAAGGCTCTTGCAAAAGATGCTGATGAAATCGTCCCAAAGGTGATTTCTGCTGCCCAAAGTGTTGAAATTGTTGAAGGAAATGGAGGACCCGGAACTATTAAGAAGCTATCCATTGTTGAAGGTAATTCAGATCATATACTATGTAGAAATATATAATACTTGAGATCAAATGATACATAGTGTCCGCGTAAAATTGTTTTAGACCGACAACCGATCAAATTTGTCGGTACAACAAAGAATAGTGTAACTACCTTTTATTTAAAAATTGACAAACATGATGTGACAAATGGAAGAGTTGCAATTGATTAATAATTTGATTAAATGATTCACAAATATGTAAGTTAAATTCATTATACCAAAGAAAAGAAAGTTAAATTAATTTTATACCAAAAAAAAAAAAGGAAGTTAAATACAACTGTAGCAAAAAAAATAGTAATCCAATTCTATTTGGATAGTCATGAAGCCATTTATGTTATACTTTTATTTTAAAATAGAATCTTGAGAAATGAAGGAGATAATACATAGCTCGTTCCAGGGTTATGTCTAATAATTATGGACCTTCAAAAAAATGATTCAGATGATTATTGATACTCGGTAAAGTTCAAATCGATCTTTAACATAAAGAGTTTTTAGTCAAACTTTACTTATATTGAAAAAAAAATATTATATAAATAATTATAATGGTTATATTGCAGATGGCAAAACCAACTTTGTGCTACACAAATTAGATGCAGTGGATGAGGCAAACTTTGGATATAACTACAGCTTAGTGGGAGGAACAGGGTTGGATGAAAATTTAGAGAAAGTTGAATTTGAGACAAAAATTGTTGCTGGCTCTGATGGTGGATCCGTTGTTAAGATTTCAGTGAAATACCATACCAAAGGTGATGCAACTCTATCTGAAGCAGTACGTGAGGAGACTAAGGCCAAAGGAACTGGACTTATCAAGGCCATTGAGGGCTACGTCGTGGCAAACCCTAATTCTAGCAAATTAAACATTTGCATTAATTTGGTTTGTGTTGTTTCATGCGAATAA

Protein sequence

>MS.gene058174.t1

MGVFTFNDEHVSTVAPAKLYKALAKDADEIVPKVISAAQSVEIVEGNGGPGTIKKLSIVEDGKTNFVLHKLDAVDEANFGYNYSLVGGTGLDENLEKVEFETKIVAGSDGGSVVKISVKYHTKGDATLSEAVREETKAKGTGLIKAIEGYVVANPNSSKLNICINLVCVVSCE