AlfalfaGEDB | MS.gene060431

M. sativa cultivar XinJiangDaYe / MS.gene060431

KEGG: K02905
GO: GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0019538 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0071704 GO:0110165 GO:1901564 GO:1901566 GO:1901576
PFAM: PF01779.20

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene060431.t1	XP_024637125.1	96.7	61	2	0	1	61	1	61	7.60E-24	119

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene060431.t1	Q9M7X7	86.9	61	8	0	1	61	1	61	1.0e-21	103.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene060431.t1	I3SRL9	96.7	61	2	0	1	61	1	61	5.5e-24	119.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene060431	MS.gene62560	0.945205	4.72E-104	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene043645	MS.gene060431	PPI
MS.gene064353	MS.gene060431	PPI
MS.gene035826	MS.gene060431	PPI
MS.gene064351	MS.gene060431	PPI
MS.gene035825	MS.gene060431	PPI
MS.gene73093	MS.gene060431	PPI
MS.gene043643	MS.gene060431	PPI
MS.gene035828	MS.gene060431	PPI
MS.gene99872	MS.gene060431	PPI
MS.gene043595	MS.gene060431	PPI
MS.gene043593	MS.gene060431	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene060431.t1	MTR_4g121870	96.721	61	2	0	1	61	137	197	4.71e-38	124
MS.gene060431.t1	MTR_4g121877	95.082	61	3	0	1	61	1	61	4.16e-37	118

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene060431.t1	AT3G06700	86.885	61	8	0	1	61	1	61	3.03e-32	105
MS.gene060431.t1	AT3G06700	86.885	61	8	0	1	61	1	61	3.03e-32	105
MS.gene060431.t1	AT3G06700	86.885	61	8	0	1	61	1	61	3.03e-32	105
MS.gene060431.t1	AT3G06680	83.607	61	10	0	1	61	23	83	9.66e-32	105
MS.gene060431.t1	AT3G06680	83.607	61	10	0	1	61	1	61	2.47e-31	103
MS.gene060431.t1	AT3G06680	83.607	61	10	0	1	61	1	61	2.47e-31	103

Find 13 sgRNAs with CRISPR-Local

Find 19 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATGGCATCAAGAAGCCAAAA+AGG	0.372719	7.1:+66493450	MS.gene060431:CDS
TCTTGTGAGCCTTGTAAGAC+TGG	0.428046	7.1:-66493427	None:intergenic
TGTGTTTCCTCGCATACCTC+TGG	0.457559	7.1:-66493614	None:intergenic
ATCACCACACTTCCACCAAA+GGG	0.482418	7.1:+66493474	MS.gene060431:CDS
CTGAGTAATACATACCCCTT+TGG	0.488635	7.1:-66493489	None:intergenic
CGCTCACAACCAGTCTTACA+AGG	0.489194	7.1:+66493418	MS.gene060431:CDS
TCTTACAAGGCTCACAAGAA+TGG	0.521704	7.1:+66493431	MS.gene060431:CDS
AGTAATACATACCCCTTTGG+TGG	0.557801	7.1:-66493486	None:intergenic
CATCACCACACTTCCACCAA+AGG	0.580490	7.1:+66493473	MS.gene060431:CDS
TAAGGAACCAGAGGTATGCG+AGG	0.651232	7.1:+66493607	MS.gene060431:CDS
CTTGTAAGACTGGTTGTGAG+CGG	0.674336	7.1:-66493417	None:intergenic
ATACCCCTTTGGTGGAAGTG+TGG	0.685351	7.1:-66493478	None:intergenic
TCACCACACTTCCACCAAAG+GGG	0.701603	7.1:+66493475	MS.gene060431:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTTACTTTTATAATTGTAGA+TGG	+	chr7.1:66493572-66493591	MS.gene060431:intron	15.0%
	AGGAAACACAACAAGAAAAA+TGG	+	chr7.1:66493627-66493646	MS.gene060431:CDS	30.0%
!	AGATGGATCCAAAGTTTTTA+AGG	+	chr7.1:66493589-66493608	MS.gene060431:intron	30.0%
!	CTCTGGTTCCTTAAAAACTT+TGG	-	chr7.1:66493600-66493619	None:intergenic	35.0%
!!	CAAAGTTTTTAAGGAACCAG+AGG	+	chr7.1:66493598-66493617	MS.gene060431:CDS	35.0%
	AGTAATACATACCCCTTTGG+TGG	-	chr7.1:66493489-66493508	None:intergenic	40.0%
	ATGGCATCAAGAAGCCAAAA+AGG	+	chr7.1:66493450-66493469	MS.gene060431:CDS	40.0%
	CTGAGTAATACATACCCCTT+TGG	-	chr7.1:66493492-66493511	None:intergenic	40.0%
	TCTTACAAGGCTCACAAGAA+TGG	+	chr7.1:66493431-66493450	MS.gene060431:CDS	40.0%
	ATCACCACACTTCCACCAAA+GGG	+	chr7.1:66493474-66493493	MS.gene060431:CDS	45.0%
!	CTTGTAAGACTGGTTGTGAG+CGG	-	chr7.1:66493420-66493439	None:intergenic	45.0%
!	TCTTGTGAGCCTTGTAAGAC+TGG	-	chr7.1:66493430-66493449	None:intergenic	45.0%
!!	GAAGTGTGGTGATGCCTTTT+TGG	-	chr7.1:66493467-66493486	None:intergenic	45.0%
	ATACCCCTTTGGTGGAAGTG+TGG	-	chr7.1:66493481-66493500	None:intergenic	50.0%
	CATCACCACACTTCCACCAA+AGG	+	chr7.1:66493473-66493492	MS.gene060431:CDS	50.0%
	CGCTCACAACCAGTCTTACA+AGG	+	chr7.1:66493418-66493437	MS.gene060431:CDS	50.0%
	TCACCACACTTCCACCAAAG+GGG	+	chr7.1:66493475-66493494	MS.gene060431:CDS	50.0%
	TGTGTTTCCTCGCATACCTC+TGG	-	chr7.1:66493617-66493636	None:intergenic	50.0%
!	TAAGGAACCAGAGGTATGCG+AGG	+	chr7.1:66493607-66493626	MS.gene060431:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.1	gene	66493395	66493674	66493395	ID=MS.gene060431
chr7.1	mRNA	66493395	66493674	66493395	ID=MS.gene060431.t1;Parent=MS.gene060431
chr7.1	exon	66493395	66493496	66493395	ID=MS.gene060431.t1.exon1;Parent=MS.gene060431.t1
chr7.1	CDS	66493395	66493496	66493395	ID=cds.MS.gene060431.t1;Parent=MS.gene060431.t1
chr7.1	exon	66493591	66493674	66493591	ID=MS.gene060431.t1.exon2;Parent=MS.gene060431.t1
chr7.1	CDS	66493591	66493674	66493591	ID=cds.MS.gene060431.t1;Parent=MS.gene060431.t1

Gene Sequence	>MS.gene060431 ATGGCGAAGTCGAAGAATCACACCGCTCACAACCAGTCTTACAAGGCTCACAAGAATGGCATCAAGAAGCCAAAAAGGCATCACCACACTTCCACCAAAGGGGTATGTATTACTCAGTTTTCAATTCTTTTATTGATTTTGAAATGATTGATTTCTATTGATGATTAATAATTTTATTTTACTTTTATAATTGTAGATGGATCCAAAGTTTTTAAGGAACCAGAGGTATGCGAGGAAACACAACAAGAAAAATGGTGAAATCGCTTCTGCTGAAGAGTAA
Protein sequence	>MS.gene060431.t1 MAKSKNHTAHNQSYKAHKNGIKKPKRHHHTSTKGMDPKFLRNQRYARKHNKKNGEIASAEE

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