AlfalfaGEDB | MS.gene62560

M. sativa cultivar XinJiangDaYe / MS.gene62560

KEGG: K02905
GO: GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0019538 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0071704 GO:0110165 GO:1901564 GO:1901566 GO:1901576
PFAM: PF01779.20

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene62560.t1	XP_024637125.1	96.7	61	2	0	1	61	1	61	7.60E-24	119

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene62560.t1	Q9M7X7	86.9	61	8	0	1	61	1	61	1.0e-21	103.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene62560.t1	I3SRL9	96.7	61	2	0	1	61	1	61	5.5e-24	119.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene060431	MS.gene62560	0.945205	4.72E-104	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene73093	MS.gene62560	PPI
MS.gene99872	MS.gene62560	PPI
MS.gene043643	MS.gene62560	PPI
MS.gene035828	MS.gene62560	PPI
MS.gene043595	MS.gene62560	PPI
MS.gene043593	MS.gene62560	PPI
MS.gene064353	MS.gene62560	PPI
MS.gene043645	MS.gene62560	PPI
MS.gene035825	MS.gene62560	PPI
MS.gene035826	MS.gene62560	PPI
MS.gene064351	MS.gene62560	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene62560.t1	MTR_4g121870	96.721	61	2	0	1	61	137	197	4.71e-38	124
MS.gene62560.t1	MTR_4g121877	95.082	61	3	0	1	61	1	61	4.16e-37	118

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene62560.t1	AT3G06700	86.885	61	8	0	1	61	1	61	3.03e-32	105
MS.gene62560.t1	AT3G06700	86.885	61	8	0	1	61	1	61	3.03e-32	105
MS.gene62560.t1	AT3G06700	86.885	61	8	0	1	61	1	61	3.03e-32	105
MS.gene62560.t1	AT3G06680	83.607	61	10	0	1	61	23	83	9.66e-32	105
MS.gene62560.t1	AT3G06680	83.607	61	10	0	1	61	1	61	2.47e-31	103
MS.gene62560.t1	AT3G06680	83.607	61	10	0	1	61	1	61	2.47e-31	103

Find 13 sgRNAs with CRISPR-Local

Find 19 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATGGCATCAAGAAGCCAAAA+AGG	0.372719	7.3:-69027216	MS.gene62560:CDS
TCTTGTGAGCCTTGTAAGAC+TGG	0.428046	7.3:+69027239	None:intergenic
TGTGTTTCCTCGCATACCTC+TGG	0.457559	7.3:+69027052	None:intergenic
ATCACCACACTTCCACCAAA+GGG	0.482418	7.3:-69027192	MS.gene62560:CDS
CTGAGTAATACATACCCCTT+TGG	0.488635	7.3:+69027177	None:intergenic
CGCTCACAACCAGTCTTACA+AGG	0.489194	7.3:-69027248	MS.gene62560:CDS
TCTTACAAGGCTCACAAGAA+TGG	0.521704	7.3:-69027235	MS.gene62560:CDS
AGTAATACATACCCCTTTGG+TGG	0.557801	7.3:+69027180	None:intergenic
CATCACCACACTTCCACCAA+AGG	0.580490	7.3:-69027193	MS.gene62560:CDS
TAAGGAACCAGAGGTATGCG+AGG	0.651232	7.3:-69027059	MS.gene62560:CDS
CTTGTAAGACTGGTTGTGAG+CGG	0.674336	7.3:+69027249	None:intergenic
ATACCCCTTTGGTGGAAGTG+TGG	0.685351	7.3:+69027188	None:intergenic
TCACCACACTTCCACCAAAG+GGG	0.701603	7.3:-69027191	MS.gene62560:intron

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTTACTTTTATAATTGTAGA+TGG	-	chr7.3:69027191-69027210	MS.gene62560:intron	15.0%
	AGGAAACACAACAAGAAAAA+TGG	-	chr7.3:69027246-69027265	MS.gene62560:CDS	30.0%
!	AGATGGATCCAAAGTTTTTA+AGG	-	chr7.3:69027208-69027227	MS.gene62560:CDS	30.0%
!	CTCTGGTTCCTTAAAAACTT+TGG	+	chr7.3:69027219-69027238	None:intergenic	35.0%
!!	CAAAGTTTTTAAGGAACCAG+AGG	-	chr7.3:69027217-69027236	MS.gene62560:CDS	35.0%
	AGTAATACATACCCCTTTGG+TGG	+	chr7.3:69027108-69027127	None:intergenic	40.0%
	ATGGCATCAAGAAGCCAAAA+AGG	-	chr7.3:69027069-69027088	MS.gene62560:CDS	40.0%
	CTGAGTAATACATACCCCTT+TGG	+	chr7.3:69027111-69027130	None:intergenic	40.0%
	TCTTACAAGGCTCACAAGAA+TGG	-	chr7.3:69027050-69027069	MS.gene62560:CDS	40.0%
	ATCACCACACTTCCACCAAA+GGG	-	chr7.3:69027093-69027112	MS.gene62560:intron	45.0%
!	CTTGTAAGACTGGTTGTGAG+CGG	+	chr7.3:69027039-69027058	None:intergenic	45.0%
!	TCTTGTGAGCCTTGTAAGAC+TGG	+	chr7.3:69027049-69027068	None:intergenic	45.0%
!!	GAAGTGTGGTGATGCCTTTT+TGG	+	chr7.3:69027086-69027105	None:intergenic	45.0%
	ATACCCCTTTGGTGGAAGTG+TGG	+	chr7.3:69027100-69027119	None:intergenic	50.0%
	CATCACCACACTTCCACCAA+AGG	-	chr7.3:69027092-69027111	MS.gene62560:intron	50.0%
	CGCTCACAACCAGTCTTACA+AGG	-	chr7.3:69027037-69027056	MS.gene62560:CDS	50.0%
	TCACCACACTTCCACCAAAG+GGG	-	chr7.3:69027094-69027113	MS.gene62560:intron	50.0%
	TGTGTTTCCTCGCATACCTC+TGG	+	chr7.3:69027236-69027255	None:intergenic	50.0%
!	TAAGGAACCAGAGGTATGCG+AGG	-	chr7.3:69027226-69027245	MS.gene62560:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.3	gene	69027014	69027293	69027014	ID=MS.gene62560
chr7.3	mRNA	69027014	69027293	69027014	ID=MS.gene62560.t1;Parent=MS.gene62560
chr7.3	exon	69027192	69027293	69027192	ID=MS.gene62560.t1.exon1;Parent=MS.gene62560.t1
chr7.3	CDS	69027192	69027293	69027192	ID=cds.MS.gene62560.t1;Parent=MS.gene62560.t1
chr7.3	exon	69027014	69027097	69027014	ID=MS.gene62560.t1.exon2;Parent=MS.gene62560.t1
chr7.3	CDS	69027014	69027097	69027014	ID=cds.MS.gene62560.t1;Parent=MS.gene62560.t1

Gene Sequence	>MS.gene62560 ATGGCGAAGTCGAAGAATCACACCGCTCACAACCAGTCTTACAAGGCTCACAAGAATGGCATCAAGAAGCCAAAAAGGCATCACCACACTTCCACCAAAGGGGTATGTATTACTCAGTTTTCAATTCTTTTATTGATTTTGAAATGATTGATTTCTATTGATGATTAATAATTTTATTTTACTTTTATAATTGTAGATGGATCCAAAGTTTTTAAGGAACCAGAGGTATGCGAGGAAACACAACAAGAAAAATGGTGAAATCGCTTCTGCTGAAGAGTAA
Protein sequence	>MS.gene62560.t1 MAKSKNHTAHNQSYKAHKNGIKKPKRHHHTSTKGMDPKFLRNQRYARKHNKKNGEIASAEE

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