AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene061205


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene061205.t1 MTR_4g132000 84.722 72 5 1 1 72 1 66 1.30e-36 120
MS.gene061205.t1 MTR_4g132010 66.667 72 21 1 1 72 1 69 1.58e-24 90.1
MS.gene061205.t1 MTR_2g023010 46.237 93 33 5 1 90 1 79 9.58e-13 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 25 sgRNAs with CRISPR-Local

Find 128 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGGCGACATCATCCTCTTT+TGG 0.255313 8.2:-2492890 None:intergenic
TTAGAAAGGCTTAGAGCTTC+TGG 0.263068 8.2:+2491373 MS.gene061205:CDS
AGCCAACTGAAGTGTGTAAA+AGG 0.329405 8.2:+2492858 MS.gene061205:intron
ATTGATGAGTTACCGAGCTT+TGG 0.377860 8.2:+2492911 MS.gene061205:CDS
GAACGGCATGCCTTTGGCTT+CGG 0.395190 8.2:-2491289 None:intergenic
TGGAGTTTGGTGGAGAAGAC+TGG 0.395930 8.2:+2491233 MS.gene061205:CDS
GCAGCATCTTCAATGGAGTT+TGG 0.400947 8.2:+2491220 MS.gene061205:CDS
GATGATGAACGGCATGCCTT+TGG 0.417430 8.2:-2491295 None:intergenic
GATGAGTTACCGAGCTTTGG+AGG 0.463820 8.2:+2492914 MS.gene061205:CDS
AATAGATCATTCTCTTTCTG+AGG 0.510132 8.2:-2491352 None:intergenic
TTGCAAGGCAGCATCTTCAA+TGG 0.513024 8.2:+2491213 MS.gene061205:CDS
GGAGTTTGGTGGAGAAGACT+GGG 0.518380 8.2:+2491234 MS.gene061205:CDS
AAGAGAATGATCTATTAGAA+AGG 0.530405 8.2:+2491359 MS.gene061205:CDS
ATGCCGTTCATCATCAAGTA+AGG 0.531383 8.2:+2491303 MS.gene061205:CDS
TATCACTTGCCTCCAAAGCT+CGG 0.545319 8.2:-2492923 None:intergenic
AAAGCTCGGTAACTCATCAA+TGG 0.546639 8.2:-2492909 None:intergenic
GCATCTTCAATGGAGTTTGG+TGG 0.547410 8.2:+2491223 MS.gene061205:CDS
AAGGTCTTTGAACCCAAAAG+AGG 0.575654 8.2:+2492877 MS.gene061205:CDS
AACAAAGAAGCCGAAGCCAA+AGG 0.577054 8.2:+2491279 MS.gene061205:CDS
ACACCTTACTTGATGATGAA+CGG 0.600253 8.2:-2491306 None:intergenic
TTCTGGAGATGCAAGTGGGA+AGG 0.602037 8.2:+2491390 MS.gene061205:CDS
AGAGCTTCTGGAGATGCAAG+TGG 0.611256 8.2:+2491385 MS.gene061205:CDS
AACTATGGGAAACTGTTGCA+AGG 0.631074 8.2:+2491198 None:intergenic
GAGCTTCTGGAGATGCAAGT+GGG 0.649801 8.2:+2491386 MS.gene061205:CDS
GAGTTTGGTGGAGAAGACTG+GGG 0.753495 8.2:+2491235 MS.gene061205:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATATATATATATATGTAG+AGG + chr8.2:2491754-2491773 MS.gene061205:intron 10.0%
!! TATATATATATATATGTAGA+GGG + chr8.2:2491755-2491774 MS.gene061205:intron 10.0%
!! GAAAAATGGAAATTAATTAA+GGG - chr8.2:2492080-2492099 None:intergenic 15.0%
!! TGAAAAATGGAAATTAATTA+AGG - chr8.2:2492081-2492100 None:intergenic 15.0%
!!! AAAAAAGATCTAATTTTTTG+TGG - chr8.2:2492506-2492525 None:intergenic 15.0%
!!! TTTTTTGAAATTATTCATCT+GGG - chr8.2:2492825-2492844 None:intergenic 15.0%
!! GAAAAAATTAATAAGGTCTA+TGG - chr8.2:2492230-2492249 None:intergenic 20.0%
!! GTTGTTAAAATGATTGATAA+TGG - chr8.2:2492800-2492819 None:intergenic 20.0%
!! TTAAAACCCAAACAAATAAT+TGG + chr8.2:2492457-2492476 MS.gene061205:intron 20.0%
!! TTAAGTTTAGTTATGTTGAT+TGG - chr8.2:2492635-2492654 None:intergenic 20.0%
!! TTTCTTAGACAAATATCAAA+GGG - chr8.2:2492261-2492280 None:intergenic 20.0%
!!! CTTTTTTGAAATTATTCATC+TGG - chr8.2:2492826-2492845 None:intergenic 20.0%
!!! TAAGTTTAGTTATGTTGATT+GGG - chr8.2:2492634-2492653 None:intergenic 20.0%
!!! TTACCACTAACTTTTTTATT+TGG - chr8.2:2491853-2491872 None:intergenic 20.0%
!!! TTTTAATATGCACAAATTGA+AGG - chr8.2:2492742-2492761 None:intergenic 20.0%
! AAAATTAATAAGGTCTATGG+TGG - chr8.2:2492227-2492246 None:intergenic 25.0%
! AAGAAAAGGAAAACAAAGTT+AGG - chr8.2:2492336-2492355 None:intergenic 25.0%
! AAGAGAATGATCTATTAGAA+AGG + chr8.2:2491359-2491378 MS.gene061205:CDS 25.0%
! CTAAGTAATTAACAACATTC+AGG - chr8.2:2491625-2491644 None:intergenic 25.0%
! CTTAACAGTTTGGATTTATT+TGG - chr8.2:2492162-2492181 None:intergenic 25.0%
! CTTTCTTAGACAAATATCAA+AGG - chr8.2:2492262-2492281 None:intergenic 25.0%
! GCAAAAGGAGAAAAATTTAA+GGG - chr8.2:2492140-2492159 None:intergenic 25.0%
! TATATATATATGTAGAGGGT+TGG + chr8.2:2491759-2491778 MS.gene061205:intron 25.0%
! TTGATATTTGTCTAAGAAAG+AGG + chr8.2:2492262-2492281 MS.gene061205:intron 25.0%
!! AAGTAATAAAACGTACCTTT+TGG + chr8.2:2492113-2492132 MS.gene061205:intron 25.0%
AAAACTACGTACTGAATCAA+TGG - chr8.2:2491885-2491904 None:intergenic 30.0%
AAAAGGAAAACAAAGTTAGG+TGG - chr8.2:2492333-2492352 None:intergenic 30.0%
AAAGATTTCAAAGTCCGAAT+TGG + chr8.2:2491420-2491439 MS.gene061205:intron 30.0%
AAATTGAAGGAAGAAATTGC+AGG - chr8.2:2492729-2492748 None:intergenic 30.0%
AATAGATCATTCTCTTTCTG+AGG - chr8.2:2491355-2491374 None:intergenic 30.0%
GAAAAAAGAGACCAAAACAA+AGG + chr8.2:2491938-2491957 MS.gene061205:intron 30.0%
GAAAAATTTAAGGGACCAAA+AGG - chr8.2:2492131-2492150 None:intergenic 30.0%
GAAATTAATTAAGGGACCAA+TGG - chr8.2:2492072-2492091 None:intergenic 30.0%
GAATATGATTGATGAAGATG+AGG - chr8.2:2492371-2492390 None:intergenic 30.0%
GCTCCAAATAAAAAAGTTAG+TGG + chr8.2:2491847-2491866 MS.gene061205:intron 30.0%
GGCAAAAGGAGAAAAATTTA+AGG - chr8.2:2492141-2492160 None:intergenic 30.0%
GTTGTTAATTACTTAGTCTG+AGG + chr8.2:2491629-2491648 MS.gene061205:intron 30.0%
GTTTGGATTTATTTGGCAAA+AGG - chr8.2:2492155-2492174 None:intergenic 30.0%
TTGGGTCCAATTATTTGTTT+GGG - chr8.2:2492466-2492485 None:intergenic 30.0%
TTTGGGTCCAATTATTTGTT+TGG - chr8.2:2492467-2492486 None:intergenic 30.0%
! AACTTTTCATCATCTGATTC+TGG - chr8.2:2492766-2492785 None:intergenic 30.0%
!! AAGGTACGTTTTATTACTTG+TGG - chr8.2:2492112-2492131 None:intergenic 30.0%
!! AGGTACGTTTTATTACTTGT+GGG - chr8.2:2492111-2492130 None:intergenic 30.0%
!! TTCGGACTTTGAAATCTTTA+AGG - chr8.2:2491419-2491438 None:intergenic 30.0%
!! TTTTAGTTATGAACCAAACC+AGG + chr8.2:2491901-2491920 MS.gene061205:intron 30.0%
!!! CTTGAGTAGACAATGTTTTT+TGG + chr8.2:2492036-2492055 MS.gene061205:intron 30.0%
!!! TTTTGGCTTAAATGATCCAT+TGG + chr8.2:2492053-2492072 MS.gene061205:intron 30.0%
AAAACAAAGTTAGGTGGTAG+TGG - chr8.2:2492327-2492346 None:intergenic 35.0%
AAAGCAGATGAAGAAGAAGA+TGG + chr8.2:2491492-2491511 MS.gene061205:intron 35.0%
AAGAAGATGGCGAATGAATT+AGG + chr8.2:2491505-2491524 MS.gene061205:intron 35.0%
AGAAAAGGAAGGTGAAAAGA+AGG - chr8.2:2492530-2492549 None:intergenic 35.0%
ATCATCAATCTAACCTGGTT+TGG - chr8.2:2491917-2491936 None:intergenic 35.0%
CTGCAGAACAAGTTCTTTAT+CGG + chr8.2:2491530-2491549 MS.gene061205:intron 35.0%
GTTTGCAATTTCATTGTCTC+TGG - chr8.2:2491566-2491585 None:intergenic 35.0%
TAGAACATACTACACAGTTC+AGG + chr8.2:2491673-2491692 MS.gene061205:intron 35.0%
TCAGGAATAGTCTCTAACAT+TGG - chr8.2:2491607-2491626 None:intergenic 35.0%
TCCCAATAATTGAGCCAATT+CGG - chr8.2:2491437-2491456 None:intergenic 35.0%
TGATTGGGTTATCAAGTGTT+TGG - chr8.2:2492619-2492638 None:intergenic 35.0%
TGGGGTTAAACTTAACAGTT+TGG - chr8.2:2492172-2492191 None:intergenic 35.0%
TGTGGGACGAAAATGAAAAA+TGG - chr8.2:2492094-2492113 None:intergenic 35.0%
TTAACCCCAAATGTCTATTG+TGG + chr8.2:2492183-2492202 MS.gene061205:intron 35.0%
TTGGTCCACAATAGACATTT+GGG - chr8.2:2492191-2492210 None:intergenic 35.0%
TTTCGATCATCAATCTAACC+TGG - chr8.2:2491922-2491941 None:intergenic 35.0%
! TATATATGTAGAGGGTTGGT+TGG + chr8.2:2491763-2491782 MS.gene061205:intron 35.0%
! TCCGAATTGGCTCAATTATT+GGG + chr8.2:2491433-2491452 MS.gene061205:intron 35.0%
!! ACACCTTACTTGATGATGAA+CGG - chr8.2:2491309-2491328 None:intergenic 35.0%
!! TGGTGGACTATCTATAATGT+TGG - chr8.2:2492210-2492229 None:intergenic 35.0%
!! TTCTTCTTCATCTGCTTTTG+CGG - chr8.2:2491490-2491509 None:intergenic 35.0%
!!! GTTGTTGCTGTTTCGATTTT+GGG - chr8.2:2492484-2492503 None:intergenic 35.0%
AAAGCTCGGTAACTCATCAA+TGG - chr8.2:2492912-2492931 None:intergenic 40.0%
AAGGTCTTTGAACCCAAAAG+AGG + chr8.2:2492877-2492896 MS.gene061205:CDS 40.0%
AGCCAACTGAAGTGTGTAAA+AGG + chr8.2:2492858-2492877 MS.gene061205:intron 40.0%
ATGCCGTTCATCATCAAGTA+AGG + chr8.2:2491303-2491322 MS.gene061205:CDS 40.0%
ATTGATGAGTTACCGAGCTT+TGG + chr8.2:2492911-2492930 MS.gene061205:CDS 40.0%
GAAGGAAGAAATTGCAGGAT+TGG - chr8.2:2492724-2492743 None:intergenic 40.0%
GAAGGTGAACACAAGAGAAA+AGG - chr8.2:2492545-2492564 None:intergenic 40.0%
GATTGATGAAGATGAGGAAG+AGG - chr8.2:2492365-2492384 None:intergenic 40.0%
GGAAGAGGAAGATGAAGAAA+AGG - chr8.2:2492350-2492369 None:intergenic 40.0%
GTGAACACAAGAGAAAAGGA+AGG - chr8.2:2492541-2492560 None:intergenic 40.0%
GTTGGTCCACAATAGACATT+TGG - chr8.2:2492192-2492211 None:intergenic 40.0%
TGGGTTATCAAGTGTTTGGA+GGG - chr8.2:2492615-2492634 None:intergenic 40.0%
TGGTCCACAATAGACATTTG+GGG - chr8.2:2492190-2492209 None:intergenic 40.0%
TGTCTAAGAAAGAGGACTGT+TGG + chr8.2:2492270-2492289 MS.gene061205:intron 40.0%
TTAGAAAGGCTTAGAGCTTC+TGG + chr8.2:2491373-2491392 MS.gene061205:CDS 40.0%
TTGCAAACAAGCATGATCAC+TGG + chr8.2:2491578-2491597 MS.gene061205:intron 40.0%
TTGGGTTATCAAGTGTTTGG+AGG - chr8.2:2492616-2492635 None:intergenic 40.0%
! GACCTTTTACACACTTCAGT+TGG - chr8.2:2492863-2492882 None:intergenic 40.0%
! GTCCGAATTGGCTCAATTAT+TGG + chr8.2:2491432-2491451 MS.gene061205:intron 40.0%
!! TACACGACACACCTTTGTTT+TGG - chr8.2:2491952-2491971 None:intergenic 40.0%
!!! GGTTGTTGCTGTTTCGATTT+TGG - chr8.2:2492485-2492504 None:intergenic 40.0%
AACAAAGAAGCCGAAGCCAA+AGG + chr8.2:2491279-2491298 MS.gene061205:CDS 45.0%
AAGAGAGAAGGAACGGAAGA+AGG - chr8.2:2492567-2492586 None:intergenic 45.0%
AGGGAATGAATGAGGTTGTG+AGG - chr8.2:2492596-2492615 None:intergenic 45.0%
GCAGCATCTTCAATGGAGTT+TGG + chr8.2:2491220-2491239 MS.gene061205:CDS 45.0%
GCATCTTCAATGGAGTTTGG+TGG + chr8.2:2491223-2491242 MS.gene061205:CDS 45.0%
GGAACAGAAGAGAGAAGGAA+CGG - chr8.2:2492574-2492593 None:intergenic 45.0%
GGGAATGAATGAGGTTGTGA+GGG - chr8.2:2492595-2492614 None:intergenic 45.0%
GTGTTTGGAGGGAATGAATG+AGG - chr8.2:2492604-2492623 None:intergenic 45.0%
TTGCAAGGCAGCATCTTCAA+TGG + chr8.2:2491213-2491232 MS.gene061205:CDS 45.0%
! ATGGCGACATCATCCTCTTT+TGG - chr8.2:2492893-2492912 None:intergenic 45.0%
! TGGCGACATCATCCTCTTTT+GGG - chr8.2:2492892-2492911 None:intergenic 45.0%
!! AGGGTTGGTTGGTTGGTTAA+GGG + chr8.2:2491774-2491793 MS.gene061205:intron 45.0%
!! AGTTAGGTGGTAGTGGTACA+TGG - chr8.2:2492320-2492339 None:intergenic 45.0%
!! TATGTAGAGGGTTGGTTGGT+TGG + chr8.2:2491767-2491786 MS.gene061205:intron 45.0%
!! ATATAAAATAAAAAAATTCT+GGG - chr8.2:2492412-2492431 None:intergenic 5.0%
!! TAAAATTGAAAAAATTAATA+AGG - chr8.2:2492237-2492256 None:intergenic 5.0%
!! TATATAAAATAAAAAAATTC+TGG - chr8.2:2492413-2492432 None:intergenic 5.0%
GAGAAGGAACGGAAGAAGGA+AGG - chr8.2:2492563-2492582 None:intergenic 50.0%
GATGATGAACGGCATGCCTT+TGG - chr8.2:2491298-2491317 None:intergenic 50.0%
GTGAGGGAACAGAAGAGAGA+AGG - chr8.2:2492579-2492598 None:intergenic 50.0%
TCAGGTGCTAGCTAGCTGAA+GGG + chr8.2:2491691-2491710 MS.gene061205:intron 50.0%
TTCAGGTGCTAGCTAGCTGA+AGG + chr8.2:2491690-2491709 MS.gene061205:intron 50.0%
! AGAGCTTCTGGAGATGCAAG+TGG + chr8.2:2491385-2491404 MS.gene061205:CDS 50.0%
! GAGCTTCTGGAGATGCAAGT+GGG + chr8.2:2491386-2491405 MS.gene061205:CDS 50.0%
! GAGTTTGGTGGAGAAGACTG+GGG + chr8.2:2491235-2491254 MS.gene061205:CDS 50.0%
! GATGAGTTACCGAGCTTTGG+AGG + chr8.2:2492914-2492933 MS.gene061205:CDS 50.0%
! GGAGTTTGGTGGAGAAGACT+GGG + chr8.2:2491234-2491253 MS.gene061205:CDS 50.0%
! TATTCCACACGACGCCGTTT+TGG - chr8.2:2492699-2492718 None:intergenic 50.0%
! TGGAGTTTGGTGGAGAAGAC+TGG + chr8.2:2491233-2491252 MS.gene061205:CDS 50.0%
! TTCTGGAGATGCAAGTGGGA+AGG + chr8.2:2491390-2491409 MS.gene061205:CDS 50.0%
!! GAGGGTTGGTTGGTTGGTTA+AGG + chr8.2:2491773-2491792 MS.gene061205:intron 50.0%
ACCGTCCTCGCCTACCAAAA+CGG + chr8.2:2492682-2492701 MS.gene061205:intron 55.0%
! AGGCGAGGACGGTTTTGCTT+TGG - chr8.2:2492675-2492694 None:intergenic 55.0%
! GAACGGCATGCCTTTGGCTT+CGG - chr8.2:2491292-2491311 None:intergenic 55.0%
CCTACCAAAACGGCGTCGTG+TGG + chr8.2:2492692-2492711 MS.gene061205:intron 60.0%
!! CCACACGACGCCGTTTTGGT+AGG - chr8.2:2492695-2492714 None:intergenic 60.0%
!! GCCGTTTTGGTAGGCGAGGA+CGG - chr8.2:2492686-2492705 None:intergenic 60.0%
!! CGACGCCGTTTTGGTAGGCG+AGG - chr8.2:2492690-2492709 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr8.2 gene 2491202 2492943 2491202 ID=MS.gene061205
chr8.2 mRNA 2491202 2492943 2491202 ID=MS.gene061205.t1;Parent=MS.gene061205
chr8.2 exon 2491202 2491411 2491202 ID=MS.gene061205.t1.exon1;Parent=MS.gene061205.t1
chr8.2 CDS 2491202 2491411 2491202 ID=cds.MS.gene061205.t1;Parent=MS.gene061205.t1
chr8.2 exon 2492860 2492943 2492860 ID=MS.gene061205.t1.exon2;Parent=MS.gene061205.t1
chr8.2 CDS 2492860 2492943 2492860 ID=cds.MS.gene061205.t1;Parent=MS.gene061205.t1
Gene Sequence

>MS.gene061205

ATGGGAAACTGTTGCAAGGCAGCATCTTCAATGGAGTTTGGTGGAGAAGACTGGGGATCTTTGAATTCATCTAAGCAAACAAAGAAGCCGAAGCCAAAGGCATGCCGTTCATCATCAAGTAAGGTGTTTGATGAAGCTCGTCTTGATCATCCTCAGAAAGAGAATGATCTATTAGAAAGGCTTAGAGCTTCTGGAGATGCAAGTGGGAAGGTAACCTTAAAGATTTCAAAGTCCGAATTGGCTCAATTATTGGGAGCTATACAAAATAGTAGTAATAATCAACAGCCGCAAAAGCAGATGAAGAAGAAGATGGCGAATGAATTAGGTTCTGCAGAACAAGTTCTTTATCGGTTAATAAAAGCCAGAGACAATGAAATTGCAAACAAGCATGATCACTGGAAACCAATGTTAGAGACTATTCCTGAATGTTGTTAATTACTTAGTCTGAGGTTAATTAGTTCTTTAGTTAATTAGAACATACTACACAGTTCAGGTGCTAGCTAGCTGAAGGGTCGATTGAGCATTTGTTTTTTCTCAACTTTTTCATATACAATATATATATATATATGTAGAGGGTTGGTTGGTTGGTTAAGGGATCGTGTAATGTGTATGATCTTTTTCGTTTGTAAAATTGATGAAGCTGCTGCTCCAAATAAAAAAGTTAGTGGTAATAACTATTTCCATTGATTCAGTACGTAGTTTTAGTTATGAACCAAACCAGGTTAGATTGATGATCGAAAAAAGAGACCAAAACAAAGGTGTGTCGTGTAGTACTCTACATCTATTCGTCAATCACGACGAAAACTATTTATATTTATATCATATAAATCATATCTTGAGTAGACAATGTTTTTTGGCTTAAATGATCCATTGGTCCCTTAATTAATTTCCATTTTTCATTTTCGTCCCACAAGTAATAAAACGTACCTTTTGGTCCCTTAAATTTTTCTCCTTTTGCCAAATAAATCCAAACTGTTAAGTTTAACCCCAAATGTCTATTGTGGACCAACATTATAGATAGTCCACCATAGACCTTATTAATTTTTTCAATTTTAACCCTTTGATATTTGTCTAAGAAAGAGGACTGTTGGATAACATATGTCTATTTTATCTTACCATGTACCACTACCACCTAACTTTGTTTTCCTTTTCTTCATCTTCCTCTTCCTCATCTTCATCAATCATATTCATACATTGATCAAAACAACCCAGAATTTTTTTATTTTATATATATTCTTCATCATCTCAATTCACATTAAAACCCAAACAAATAATTGGACCCAAAATCGAAACAGCAACAACCACAAAAAATTAGATCTTTTTTTCCTTCTTTTCACCTTCCTTTTCTCTTGTGTTCACCTTCCTTCTTCCGTTCCTTCTCTCTTCTGTTCCCTCACAACCTCATTCATTCCCTCCAAACACTTGATAACCCAATCAACATAACTAAACTTAAACTTCGAATCAGTAACTCCAAAGCAAAACCGTCCTCGCCTACCAAAACGGCGTCGTGTGGAATAATCCAATCCTGCAATTTCTTCCTTCAATTTGTGCATATTAAAATCCAGAATCAGATGATGAAAAGTTTATTTTGTTATCCATTATCAATCATTTTAACAACGACCCAGATGAATAATTTCAAAAAAGATTAAAAATTAAAGCCAACTGAAGTGTGTAAAAGGTCTTTGAACCCAAAAGAGGATGATGTCGCCATTGATGAGTTACCGAGCTTTGGAGGCAAGTGA

Protein sequence

>MS.gene061205.t1

MGNCCKAASSMEFGGEDWGSLNSSKQTKKPKPKACRSSSSKVFDEARLDHPQKENDLLERLRASGDASGKPTEVCKRSLNPKEDDVAIDELPSFGGK