AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene51664


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene51664.t1 MTR_3g010290 97.297 259 7 0 1 259 1 259 0.0 523
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene51664.t1 AT4G10170 53.462 260 94 5 1 259 1 234 2.53e-82 248
MS.gene51664.t1 AT4G10170 53.462 260 94 5 1 259 1 234 2.53e-82 248
MS.gene51664.t1 AT1G33475 43.561 264 117 8 1 260 1 236 1.18e-61 195
MS.gene51664.t1 AT1G33475 57.143 56 22 1 1 54 1 56 1.44e-16 75.1
MS.gene51664.t1 AT1G33475 42.857 91 40 3 171 260 55 134 9.59e-15 70.1

Find 58 sgRNAs with CRISPR-Local

Find 72 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTTCTTTGCAAAAGGATA+TGG 0.148205 3.4:-5226167 MS.gene51664:CDS
AAGGCCTGATTCAGGAAATT+TGG 0.252155 3.4:-5226221 MS.gene51664:CDS
TTCAACCAAAGCCCCTTTAT+TGG 0.253713 3.4:-5226344 MS.gene51664:CDS
TTTCACATAGTGGTAGTAAT+TGG 0.275380 3.4:-5226447 MS.gene51664:CDS
AATGAGAGATGTTGAGTTAG+AGG 0.307965 3.4:-5226272 MS.gene51664:CDS
TCAACCAAAGCCCCTTTATT+GGG 0.328565 3.4:-5226343 MS.gene51664:CDS
GACGAAACTTCCTCGACATT+TGG 0.372647 3.4:-5226421 MS.gene51664:CDS
TCGTCCAAATTTCCTGAATC+AGG 0.373685 3.4:+5226217 None:intergenic
ATTTCGAGACAATAGGTAAA+AGG 0.375196 3.4:-5226690 MS.gene51664:CDS
AGTTTACGTTCAAGAGAAAC+TGG 0.388560 3.4:-5226509 MS.gene51664:CDS
AGAGGATCAAGGCCTGATTC+AGG 0.392539 3.4:-5226229 MS.gene51664:CDS
ACGTAAACTGAATTCATGTT+TGG 0.402139 3.4:+5226523 None:intergenic
ATTTGTCCATTCAAATTACT+TGG 0.402162 3.4:+5226379 None:intergenic
TACATCTTCTTCTTTGCAAA+AGG 0.409637 3.4:-5226173 MS.gene51664:CDS
AGATGAAGGTCTTGGTAACT+TGG 0.412330 3.4:-5226620 MS.gene51664:CDS
TCTTCTTTGCAAAAGGATAT+GGG 0.415200 3.4:-5226166 MS.gene51664:CDS
TAGCTAGAACAATGCGTACT+TGG 0.417134 3.4:+5226092 None:intergenic
CTCAAAACGTCCGTAAGAAA+TGG 0.447780 3.4:-5226120 MS.gene51664:CDS
TACTTCACAATTGTAGATGA+AGG 0.455781 3.4:-5226634 MS.gene51664:CDS
CATTACTGTTGTGTTTCTAA+AGG 0.456727 3.4:-5226808 MS.gene51664:CDS
CCATTCAAATTACTTGGTGA+TGG 0.462379 3.4:+5226385 None:intergenic
CACAGCCTTGTGCTTGGAGG+CGG 0.462743 3.4:-5226734 MS.gene51664:CDS
ACAATTGTAGATGAAGGTCT+TGG 0.466801 3.4:-5226628 MS.gene51664:CDS
ACAATAGGTAAAAGGACTTA+CGG 0.467377 3.4:-5226682 MS.gene51664:CDS
CTTGGCGCCACCATTTCTTA+CGG 0.469508 3.4:+5226110 None:intergenic
CAATAGGTAAAAGGACTTAC+GGG 0.479243 3.4:-5226681 MS.gene51664:CDS
TTAGAGAATGTTTCACATAG+TGG 0.480167 3.4:-5226457 MS.gene51664:CDS
AGGCGGTTCCTTCTTTCCAC+AGG 0.481926 3.4:-5226717 MS.gene51664:CDS
CACATCCTGTATGCGTATAG+TGG 0.484986 3.4:-5226781 MS.gene51664:CDS
AAATTACTTGGTGATGGAGA+TGG 0.492575 3.4:+5226391 None:intergenic
TTCAGAAAGGTAGCTAGAAA+AGG 0.498079 3.4:-5226562 MS.gene51664:CDS
AGGTGGTATTTCGAGACAAT+AGG 0.503747 3.4:-5226697 MS.gene51664:CDS
GGTTCAATGAGGACGAGATC+AGG 0.506463 3.4:-5226145 MS.gene51664:CDS
CTCCACCACTATACGCATAC+AGG 0.508766 3.4:+5226776 None:intergenic
AGGTAGCTAGAAAAGGTTCA+AGG 0.518283 3.4:-5226555 MS.gene51664:CDS
CGGTTCCTTCTTTCCACAGG+TGG 0.523709 3.4:-5226714 MS.gene51664:CDS
CCATCACCAAGTAATTTGAA+TGG 0.529421 3.4:-5226385 MS.gene51664:CDS
AAATACCACCTGTGGAAAGA+AGG 0.529530 3.4:+5226709 None:intergenic
GTGATCAGATTACGAATGAC+AGG 0.539134 3.4:+5226484 None:intergenic
ACTTCCTCGACATTTGGCAT+TGG 0.540398 3.4:-5226415 MS.gene51664:CDS
GCAAGAGAAGAAGAAAGTAA+AGG 0.545957 3.4:-5226308 MS.gene51664:CDS
CACTATACGCATACAGGATG+TGG 0.545979 3.4:+5226782 None:intergenic
TGTCACAGCCTTGTGCTTGG+AGG 0.553859 3.4:-5226737 MS.gene51664:CDS
GAATGTCACAGCCTTGTGCT+TGG 0.555477 3.4:-5226740 MS.gene51664:CDS
AATCTACAGATAGAGGATCA+AGG 0.570567 3.4:-5226240 MS.gene51664:CDS
GGTAGCTAGAAAAGGTTCAA+GGG 0.580926 3.4:-5226554 MS.gene51664:CDS
AAAACGTCCGTAAGAAATGG+TGG 0.598377 3.4:-5226117 MS.gene51664:CDS
TTGTCTCGAAATACCACCTG+TGG 0.605879 3.4:+5226701 None:intergenic
AGGAACCGCCTCCAAGCACA+AGG 0.610638 3.4:+5226729 None:intergenic
GTAGCTAGAAAAGGTTCAAG+GGG 0.613162 3.4:-5226553 MS.gene51664:CDS
AAGATGTAGCAGCACCACCT+TGG 0.620183 3.4:+5226188 None:intergenic
TGTCAGAGATGAATTCAGAA+AGG 0.624051 3.4:-5226575 MS.gene51664:CDS
ATCCTGTATGCGTATAGTGG+TGG 0.628603 3.4:-5226778 MS.gene51664:CDS
GTAAATGATCAGATCAGGCA+CGG 0.633105 3.4:+5225935 None:intergenic
AATTTGGACGATATTAGCCA+AGG 0.644044 3.4:-5226205 MS.gene51664:CDS
TGGACCAATGCCAAATGTCG+AGG 0.656618 3.4:+5226411 None:intergenic
TTGGACGATATTAGCCAAGG+TGG 0.671852 3.4:-5226202 MS.gene51664:CDS
AAAAGGATATGGGTTCAATG+AGG 0.721400 3.4:-5226156 MS.gene51664:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GTATAATATTATTTGTCATC+TGG - chr3.4:5226726-5226745 MS.gene51664:CDS 20.0%
! AATACGATAACGATCAAATT+CGG + chr3.4:5226810-5226829 None:intergenic 25.0%
! ATTTGTCCATTCAAATTACT+TGG + chr3.4:5226404-5226423 None:intergenic 25.0%
! CATAAAAAATCTACAGATAG+AGG - chr3.4:5226533-5226552 MS.gene51664:CDS 25.0%
!! TACTAGATTTTCCCAATAAA+GGG + chr3.4:5226451-5226470 None:intergenic 25.0%
!! TTACTAGATTTTCCCAATAA+AGG + chr3.4:5226452-5226471 None:intergenic 25.0%
ACAATAGGTAAAAGGACTTA+CGG - chr3.4:5226098-5226117 MS.gene51664:CDS 30.0%
ACGTAAACTGAATTCATGTT+TGG + chr3.4:5226260-5226279 None:intergenic 30.0%
ATTTCGAGACAATAGGTAAA+AGG - chr3.4:5226090-5226109 MS.gene51664:CDS 30.0%
GAATTTGATCGTTATCGTAT+TGG - chr3.4:5226809-5226828 MS.gene51664:CDS 30.0%
TACATCTTCTTCTTTGCAAA+AGG - chr3.4:5226607-5226626 MS.gene51664:CDS 30.0%
TACTTCACAATTGTAGATGA+AGG - chr3.4:5226146-5226165 MS.gene51664:CDS 30.0%
TCTTCTTTGCAAAAGGATAT+GGG - chr3.4:5226614-5226633 MS.gene51664:CDS 30.0%
TTAGAGAATGTTTCACATAG+TGG - chr3.4:5226323-5226342 MS.gene51664:CDS 30.0%
TTCTTCTTTGCAAAAGGATA+TGG - chr3.4:5226613-5226632 MS.gene51664:CDS 30.0%
TTTCACATAGTGGTAGTAAT+TGG - chr3.4:5226333-5226352 MS.gene51664:CDS 30.0%
! ACTAGATTTTCCCAATAAAG+GGG + chr3.4:5226450-5226469 None:intergenic 30.0%
! CATTACTGTTGTGTTTCTAA+AGG - chr3.4:5225972-5225991 MS.gene51664:intron 30.0%
AAAAGGATATGGGTTCAATG+AGG - chr3.4:5226624-5226643 MS.gene51664:CDS 35.0%
AATCTACAGATAGAGGATCA+AGG - chr3.4:5226540-5226559 MS.gene51664:CDS 35.0%
AATGAGAGATGTTGAGTTAG+AGG - chr3.4:5226508-5226527 MS.gene51664:CDS 35.0%
AATTTGGACGATATTAGCCA+AGG - chr3.4:5226575-5226594 MS.gene51664:CDS 35.0%
ACAATTGTAGATGAAGGTCT+TGG - chr3.4:5226152-5226171 MS.gene51664:CDS 35.0%
AGTTTACGTTCAAGAGAAAC+TGG - chr3.4:5226271-5226290 MS.gene51664:CDS 35.0%
ATCGAATGCATGATATACCA+CGG + chr3.4:5226763-5226782 None:intergenic 35.0%
CAATAGGTAAAAGGACTTAC+GGG - chr3.4:5226099-5226118 MS.gene51664:CDS 35.0%
CCATCACCAAGTAATTTGAA+TGG - chr3.4:5226395-5226414 MS.gene51664:CDS 35.0%
GCAAGAGAAGAAGAAAGTAA+AGG - chr3.4:5226472-5226491 MS.gene51664:CDS 35.0%
TGTCAGAGATGAATTCAGAA+AGG - chr3.4:5226205-5226224 MS.gene51664:CDS 35.0%
TTCAGAAAGGTAGCTAGAAA+AGG - chr3.4:5226218-5226237 MS.gene51664:CDS 35.0%
! ATTTCTTACGGACGTTTTGA+GGG + chr3.4:5226661-5226680 None:intergenic 35.0%
! CCATTCAAATTACTTGGTGA+TGG + chr3.4:5226398-5226417 None:intergenic 35.0%
!! AAATTACTTGGTGATGGAGA+TGG + chr3.4:5226392-5226411 None:intergenic 35.0%
!! CTTGGTAGTTCTTCAGTTTT+TGG - chr3.4:5226178-5226197 MS.gene51664:CDS 35.0%
AAAACGTCCGTAAGAAATGG+TGG - chr3.4:5226663-5226682 MS.gene51664:CDS 40.0%
AAATACCACCTGTGGAAAGA+AGG + chr3.4:5226074-5226093 None:intergenic 40.0%
AAGGCCTGATTCAGGAAATT+TGG - chr3.4:5226559-5226578 MS.gene51664:CDS 40.0%
AGGTAGCTAGAAAAGGTTCA+AGG - chr3.4:5226225-5226244 MS.gene51664:CDS 40.0%
AGGTGGTATTTCGAGACAAT+AGG - chr3.4:5226083-5226102 MS.gene51664:CDS 40.0%
ATCTGGTTAGTAATATGCCG+TGG - chr3.4:5226743-5226762 MS.gene51664:CDS 40.0%
CTCAAAACGTCCGTAAGAAA+TGG - chr3.4:5226660-5226679 MS.gene51664:CDS 40.0%
GGTAGCTAGAAAAGGTTCAA+GGG - chr3.4:5226226-5226245 MS.gene51664:CDS 40.0%
GTAGCTAGAAAAGGTTCAAG+GGG - chr3.4:5226227-5226246 MS.gene51664:CDS 40.0%
GTGATCAGATTACGAATGAC+AGG + chr3.4:5226299-5226318 None:intergenic 40.0%
TAGCTAGAACAATGCGTACT+TGG + chr3.4:5226691-5226710 None:intergenic 40.0%
TCAACCAAAGCCCCTTTATT+GGG - chr3.4:5226437-5226456 MS.gene51664:CDS 40.0%
TCGTCCAAATTTCCTGAATC+AGG + chr3.4:5226566-5226585 None:intergenic 40.0%
TTCAACCAAAGCCCCTTTAT+TGG - chr3.4:5226436-5226455 MS.gene51664:CDS 40.0%
! AGATGAAGGTCTTGGTAACT+TGG - chr3.4:5226160-5226179 MS.gene51664:CDS 40.0%
! CATTTCTTACGGACGTTTTG+AGG + chr3.4:5226662-5226681 None:intergenic 40.0%
! TTTTCCCAATAAAGGGGCTT+TGG + chr3.4:5226444-5226463 None:intergenic 40.0%
!! AAGGACTTACGGGTTTTTAG+TGG - chr3.4:5226109-5226128 MS.gene51664:CDS 40.0%
!!! TACGGGTTTTTAGTGGAAGA+TGG - chr3.4:5226116-5226135 MS.gene51664:CDS 40.0%
ACTTCCTCGACATTTGGCAT+TGG - chr3.4:5226365-5226384 MS.gene51664:CDS 45.0%
ATCCTGTATGCGTATAGTGG+TGG - chr3.4:5226002-5226021 MS.gene51664:intron 45.0%
CACATCCTGTATGCGTATAG+TGG - chr3.4:5225999-5226018 MS.gene51664:intron 45.0%
CACTATACGCATACAGGATG+TGG + chr3.4:5226001-5226020 None:intergenic 45.0%
GACGAAACTTCCTCGACATT+TGG - chr3.4:5226359-5226378 MS.gene51664:CDS 45.0%
TTGGACGATATTAGCCAAGG+TGG - chr3.4:5226578-5226597 MS.gene51664:CDS 45.0%
TTGTCTCGAAATACCACCTG+TGG + chr3.4:5226082-5226101 None:intergenic 45.0%
AAGATGTAGCAGCACCACCT+TGG + chr3.4:5226595-5226614 None:intergenic 50.0%
AGAGGATCAAGGCCTGATTC+AGG - chr3.4:5226551-5226570 MS.gene51664:CDS 50.0%
CTCCACCACTATACGCATAC+AGG + chr3.4:5226007-5226026 None:intergenic 50.0%
CTTGGCGCCACCATTTCTTA+CGG + chr3.4:5226673-5226692 None:intergenic 50.0%
GGTTCAATGAGGACGAGATC+AGG - chr3.4:5226635-5226654 MS.gene51664:CDS 50.0%
TGGACCAATGCCAAATGTCG+AGG + chr3.4:5226372-5226391 None:intergenic 50.0%
! GAATGTCACAGCCTTGTGCT+TGG - chr3.4:5226040-5226059 MS.gene51664:intron 50.0%
AGGCGGTTCCTTCTTTCCAC+AGG - chr3.4:5226063-5226082 MS.gene51664:intron 55.0%
CGGTTCCTTCTTTCCACAGG+TGG - chr3.4:5226066-5226085 MS.gene51664:intron 55.0%
! TGTCACAGCCTTGTGCTTGG+AGG - chr3.4:5226043-5226062 MS.gene51664:intron 55.0%
AGGAACCGCCTCCAAGCACA+AGG + chr3.4:5226054-5226073 None:intergenic 60.0%
! CACAGCCTTGTGCTTGGAGG+CGG - chr3.4:5226046-5226065 MS.gene51664:intron 60.0%


Chromosome Type Strat End Strand Name
chr3.4 gene 5225948 5226854 5225948 ID=MS.gene51664
chr3.4 mRNA 5225948 5226854 5225948 ID=MS.gene51664.t1;Parent=MS.gene51664
chr3.4 exon 5226081 5226854 5226081 ID=MS.gene51664.t1.exon1;Parent=MS.gene51664.t1
chr3.4 CDS 5226081 5226854 5226081 ID=cds.MS.gene51664.t1;Parent=MS.gene51664.t1
chr3.4 exon 5225948 5225962 5225948 ID=MS.gene51664.t1.exon2;Parent=MS.gene51664.t1
chr3.4 CDS 5225948 5225962 5225948 ID=cds.MS.gene51664.t1;Parent=MS.gene51664.t1
Gene Sequence

>MS.gene51664

ATGGATTCAATTCAAAATAAAGTTCATTACTGTTGTGTTTCTAAAGGTAGCCACATCCTGTATGCGTATAGTGGTGGAGATAAAGAAGTTGAGAATGTCACAGCCTTGTGCTTGGAGGCGGTTCCTTCTTTCCACAGGTGGTATTTCGAGACAATAGGTAAAAGGACTTACGGGTTTTTAGTGGAAGATGGATATGTTTACTTCACAATTGTAGATGAAGGTCTTGGTAACTTGGTAGTTCTTCAGTTTTTGGAACATGTCAGAGATGAATTCAGAAAGGTAGCTAGAAAAGGTTCAAGGGGATTTTCGCCAAACATGAATTCAGTTTACGTTCAAGAGAAACTGGTTCCTGTCATTCGTAATCTGATCACTTCATTAGAGAATGTTTCACATAGTGGTAGTAATTGGAGAGACGAAACTTCCTCGACATTTGGCATTGGTCCATCTCCATCACCAAGTAATTTGAATGGACAAATTGAAAATGTTGTTTCAACCAAAGCCCCTTTATTGGGAAAATCTAGTAAGCAAGAGAAGAAGAAAGTAAAGGATCATGTGATTGCAATGAGAGATGTTGAGTTAGAGGAGCATAAAAAATCTACAGATAGAGGATCAAGGCCTGATTCAGGAAATTTGGACGATATTAGCCAAGGTGGTGCTGCTACATCTTCTTCTTTGCAAAAGGATATGGGTTCAATGAGGACGAGATCAGGCCCTCAAAACGTCCGTAAGAAATGGTGGCGCCAAGTACGCATTGTTCTAGCTATCGATGCAGCTGTATGTATAATATTATTTGTCATCTGGTTAGTAATATGCCGTGGTATATCATGCATTCGATAGAACTCAATCATCTTATGTGACTCCGAATTTGATCGTTATCGTATTGGAAGTCTAGATTATCCGTGCCTGA

Protein sequence

>MS.gene51664.t1

MDSIQNKVHYCCVSKGSHILYAYSGGDKEVENVTALCLEAVPSFHRWYFETIGKRTYGFLVEDGYVYFTIVDEGLGNLVVLQFLEHVRDEFRKVARKGSRGFSPNMNSVYVQEKLVPVIRNLITSLENVSHSGSNWRDETSSTFGIGPSPSPSNLNGQIENVVSTKAPLLGKSSKQEKKKVKDHVIAMRDVELEEHKKSTDRGSRPDSGNLDDISQGGAATSSSLQKDMGSMRTRSGPQNVRKKWWRQVRIVLAIDAAIIRA