AlfalfaGEDB | MS.gene061485

M. sativa cultivar XinJiangDaYe / MS.gene061485

KEGG: K02357
GO: GO:0003674 GO:0003676 GO:0003723 GO:0003746 GO:0005488 GO:0006414 GO:0008135 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0034645 GO:0043170 GO:0044237 GO:0044249 GO:0044260 GO:0045182 GO:0071704 GO:0090079 GO:0097159 GO:1901363 GO:1901576
PFAM: PF00889.22

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene061485.t1	XP_003620654.1	92.1	89	7	0	1	89	945	1033	5.80E-34	153.7

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene061485.t1	A2BW76	66.3	89	30	0	1	89	100	188	1.9e-25	116.7

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene061485.t1	G7KPU0	92.1	89	7	0	1	89	945	1033	4.2e-34	153.7

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene051198	MS.gene061485	0.80795	3.93E-50	-1.69E-46
MS.gene051279	MS.gene061485	0.817846	2.67E-52	-1.69E-46
MS.gene052519	MS.gene061485	0.812382	4.36E-51	-1.69E-46
MS.gene052584	MS.gene061485	0.802015	6.85E-49	-1.69E-46
MS.gene054236	MS.gene061485	0.805691	1.18E-49	-1.69E-46
MS.gene054651	MS.gene061485	0.821931	3.10E-53	-1.69E-46
MS.gene054775	MS.gene061485	0.817653	2.95E-52	-1.69E-46
MS.gene054801	MS.gene061485	0.813509	2.47E-51	-1.69E-46
MS.gene055228	MS.gene061485	0.811883	5.60E-51	-1.69E-46
MS.gene055357	MS.gene061485	0.812101	5.02E-51	-1.69E-46
MS.gene056050	MS.gene061485	0.803168	3.96E-49	-1.69E-46
MS.gene057444	MS.gene061485	0.804122	2.51E-49	-1.69E-46
MS.gene059272	MS.gene061485	0.806561	7.75E-50	-1.69E-46
MS.gene059276	MS.gene061485	0.807888	4.06E-50	-1.69E-46
MS.gene059601	MS.gene061485	0.842043	3.29E-58	-1.69E-46
MS.gene059602	MS.gene061485	0.906092	2.15E-80	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene061485.t1	MTR_6g088270	91.111	90	8	0	1	90	945	1034	1.19e-47	163
MS.gene061485.t1	MTR_6g088270	59.091	110	44	1	1	110	704	812	8.62e-36	129
MS.gene061485.t1	MTR_6g088240	88.235	85	10	0	5	89	90	174	2.40e-45	145
MS.gene061485.t1	MTR_2g039780	86.207	87	11	1	1	86	107	193	2.30e-43	141
MS.gene061485.t1	MTR_6g087300	83.750	80	13	0	3	82	278	357	1.08e-36	128
MS.gene061485.t1	MTR_6g084360	88.333	60	7	0	30	89	1037	1096	1.10e-26	103
MS.gene061485.t1	MTR_6g027070	65.455	55	19	0	1	55	89	143	7.08e-16	69.7

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene061485.t1	AT4G29060	75.556	90	22	1	90	852	941	1.20e-37	135
MS.gene061485.t1	AT4G29060	71.111	90	26	1	90	614	703	2.18e-34	125
MS.gene061485.t1	AT4G29060	77.108	83	19	8	90	615	697	8.69e-35	126

Find 23 sgRNAs with CRISPR-Local

Find 26 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGGTCTTCTCTTTGCATTTC+AGG	0.133913	6.1:+77218937	None:intergenic
TTCCTTCGTCACAATAATTT+CGG	0.214145	6.1:+77218966	None:intergenic
TATAGAGCAGCCTTTCATTA+AGG	0.293654	6.1:-77218839	MS.gene061485:CDS
TCCTTCGTCACAATAATTTC+GGG	0.355650	6.1:+77218967	None:intergenic
GCAAGTTGCTGCTTGTCCTC+AGG	0.414009	6.1:-77219016	MS.gene061485:CDS
GGAAGAATCTCAAAGAGGTT+AGG	0.441763	6.1:-77218874	MS.gene061485:CDS
ATAAGAGAGAAAATTGTTGA+GGG	0.490179	6.1:-77218895	MS.gene061485:CDS
GCATTTAATGTTCTCTCCAA+TGG	0.496497	6.1:+77218771	None:intergenic
TCAAATGAGCTGTGCTGCTG+TGG	0.512981	6.1:+77218709	None:intergenic
CATAAGAGAGAAAATTGTTG+AGG	0.519674	6.1:-77218896	MS.gene061485:CDS
TGTTCTCGGGTTTCAAAGCA+AGG	0.540905	6.1:+77218917	None:intergenic
ATATCTTCAATAGATACAAA+CGG	0.551658	6.1:+77218991	None:intergenic
AGGCAAAGTATAGCTGCCAT+TGG	0.552004	6.1:-77218787	MS.gene061485:CDS
GCATAAGGCGAACTATATGT+TGG	0.559489	6.1:-77218681	MS.gene061485:CDS
TCCCGAAATTATTGTGACGA+AGG	0.563159	6.1:-77218968	MS.gene061485:CDS
GCTCATTTGACAGAAGCATA+AGG	0.569196	6.1:-77218696	MS.gene061485:CDS
ACACTGTCATCCTTAATGAA+AGG	0.591158	6.1:+77218829	None:intergenic
TTTCAGTGAAAGACTTGGTG+AGG	0.596572	6.1:-77218807	MS.gene061485:CDS
TTGAGGGAAGAATCTCAAAG+AGG	0.636699	6.1:-77218879	MS.gene061485:CDS
CAGTGTTTCAGTGAAAGACT+TGG	0.652429	6.1:-77218812	MS.gene061485:CDS
ATAGATACAAACGGAACCTG+AGG	0.668908	6.1:+77219000	None:intergenic
GGTTCACTCTCTGTAAAACA+TGG	0.671192	6.1:-77218736	MS.gene061485:CDS
TCACTCTCTGTAAAACATGG+TGG	0.691839	6.1:-77218733	MS.gene061485:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATATCTTCAATAGATACAAA+CGG	+	chr6.1:77218690-77218709	None:intergenic	20.0%
!	ATAAGAGAGAAAATTGTTGA+GGG	-	chr6.1:77218783-77218802	MS.gene061485:CDS	25.0%
!!	AATTTTCTCTCTTATGTTCT+CGG	+	chr6.1:77218778-77218797	None:intergenic	25.0%
!!!	ATTAAATGCGTCATTTTGTT+CGG	-	chr6.1:77218921-77218940	MS.gene061485:CDS	25.0%
	CATAAGAGAGAAAATTGTTG+AGG	-	chr6.1:77218782-77218801	MS.gene061485:CDS	30.0%
	TTCCTTCGTCACAATAATTT+CGG	+	chr6.1:77218715-77218734	None:intergenic	30.0%
!	ATTTTCTCTCTTATGTTCTC+GGG	+	chr6.1:77218777-77218796	None:intergenic	30.0%
	ACACTGTCATCCTTAATGAA+AGG	+	chr6.1:77218852-77218871	None:intergenic	35.0%
	GCATTTAATGTTCTCTCCAA+TGG	+	chr6.1:77218910-77218929	None:intergenic	35.0%
	TATAGAGCAGCCTTTCATTA+AGG	-	chr6.1:77218839-77218858	MS.gene061485:CDS	35.0%
	TCCTTCGTCACAATAATTTC+GGG	+	chr6.1:77218714-77218733	None:intergenic	35.0%
	AGGTCTTCTCTTTGCATTTC+AGG	+	chr6.1:77218744-77218763	None:intergenic	40.0%
	ATAGATACAAACGGAACCTG+AGG	+	chr6.1:77218681-77218700	None:intergenic	40.0%
	CAGTGTTTCAGTGAAAGACT+TGG	-	chr6.1:77218866-77218885	MS.gene061485:CDS	40.0%
	GCATAAGGCGAACTATATGT+TGG	-	chr6.1:77218997-77219016	MS.gene061485:CDS	40.0%
	GGAAGAATCTCAAAGAGGTT+AGG	-	chr6.1:77218804-77218823	MS.gene061485:CDS	40.0%
	GGTTCACTCTCTGTAAAACA+TGG	-	chr6.1:77218942-77218961	MS.gene061485:CDS	40.0%
	TCACTCTCTGTAAAACATGG+TGG	-	chr6.1:77218945-77218964	MS.gene061485:CDS	40.0%
	TCCCGAAATTATTGTGACGA+AGG	-	chr6.1:77218710-77218729	MS.gene061485:CDS	40.0%
	TTGAGGGAAGAATCTCAAAG+AGG	-	chr6.1:77218799-77218818	MS.gene061485:CDS	40.0%
!	GCTCATTTGACAGAAGCATA+AGG	-	chr6.1:77218982-77219001	MS.gene061485:CDS	40.0%
!!	TTTCAGTGAAAGACTTGGTG+AGG	-	chr6.1:77218871-77218890	MS.gene061485:CDS	40.0%
	AGGCAAAGTATAGCTGCCAT+TGG	-	chr6.1:77218891-77218910	MS.gene061485:CDS	45.0%
	TGTTCTCGGGTTTCAAAGCA+AGG	+	chr6.1:77218764-77218783	None:intergenic	45.0%
!	TCAAATGAGCTGTGCTGCTG+TGG	+	chr6.1:77218972-77218991	None:intergenic	50.0%
!	GCAAGTTGCTGCTTGTCCTC+AGG	-	chr6.1:77218662-77218681	MS.gene061485:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr6.1	gene	77218660	77219040	77218660	ID=MS.gene061485
chr6.1	mRNA	77218660	77219040	77218660	ID=MS.gene061485.t1;Parent=MS.gene061485
chr6.1	exon	77218660	77219040	77218660	ID=MS.gene061485.t1.exon1;Parent=MS.gene061485.t1
chr6.1	CDS	77218660	77219040	77218660	ID=cds.MS.gene061485.t1;Parent=MS.gene061485.t1

Gene Sequence

>MS.gene061485

ATGCAAGTTGCTGCTTGTCCTCAGGTTCCGTTTGTATCTATTGAAGATATTCCCGAAATTATTGTGACGAAGGAAAAAGAACCTGAAATGCAAAGAGAAGACCTTGCTTTGAAACCCGAGAACATAAGAGAGAAAATTGTTGAGGGAAGAATCTCAAAGAGGTTAGGAGATCTTGCTCTTATAGAGCAGCCTTTCATTAAGGATGACAGTGTTTCAGTGAAAGACTTGGTGAGGCAAAGTATAGCTGCCATTGGAGAGAACATTAAATGCGTCATTTTGTTCGGTTCACTCTCTGTAAAACATGGTGGACCACAGCAGCACAGCTCATTTGACAGAAGCATAAGGCGAACTATATGTTGGTTTTGTTCAATGTCAAGTTAA

Protein sequence

>MS.gene061485.t1

MQVAACPQVPFVSIEDIPEIIVTKEKEPEMQREDLALKPENIREKIVEGRISKRLGDLALIEQPFIKDDSVSVKDLVRQSIAAIGENIKCVILFGSLSVKHGGPQQHSSFDRSIRRTICWFCSMSS