AlfalfaGEDB | MS.gene061901

M. sativa cultivar XinJiangDaYe / MS.gene061901

KEGG: K11252
GO: GO:0000786 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0032991 GO:0032993 GO:0044815 GO:0046982 GO:0046983 GO:0097159 GO:1901363
PFAM: PF00125.27

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene061901.t1	XP_020212665.1	98.6	138	2	0	1	138	1	138	3.10E-41	177.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene061901.t1	Q1SU99	99.3	138	1	0	1	138	1	138	2.2e-43	176.4

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene061901.t1	A0A151TUN5	98.6	138	2	0	1	138	1	138	2.3e-41	177.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene058976	MS.gene061901	0.819336	1.23E-52	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene061901	MS.gene025035	PPI
MS.gene061901	MS.gene24295	PPI
MS.gene061901	MS.gene26950	PPI
MS.gene061901	MS.gene63143	PPI
MS.gene061901	MS.gene81051	PPI
MS.gene061901	MS.gene65007	PPI
MS.gene061901	MS.gene63953	PPI
MS.gene061901	MS.gene84498	PPI
MS.gene061901	MS.gene27326	PPI
MS.gene066348	MS.gene061901	PPI
MS.gene061901	MS.gene066301	PPI
MS.gene061901	MS.gene049822	PPI
MS.gene061901	MS.gene051008	PPI
MS.gene061901	MS.gene025425	PPI
MS.gene061901	MS.gene035396	PPI
MS.gene061901	MS.gene65249	PPI
MS.gene061901	MS.gene08465	PPI
MS.gene061901	MS.gene99525	PPI
MS.gene061901	MS.gene75219	PPI
MS.gene061901	MS.gene004127	PPI
MS.gene061901	MS.gene025426	PPI
MS.gene061901	MS.gene004126	PPI
MS.gene061901	MS.gene64653	PPI
MS.gene061901	MS.gene066300	PPI
MS.gene061901	MS.gene049824	PPI
MS.gene061901	MS.gene041623	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene061901.t1	MTR_7g099960	99.275	138	1	0	1	138	1	138	1.85e-94	268
MS.gene061901.t1	MTR_4g063240	85.811	148	10	3	1	138	1	147	2.72e-81	236
MS.gene061901.t1	MTR_1g090780	93.525	139	6	3	1	138	1	137	6.25e-79	229
MS.gene061901.t1	MTR_1g492910	93.525	139	6	3	1	138	1	137	6.82e-79	229
MS.gene061901.t1	MTR_4g063220	86.577	149	8	3	1	138	1	148	1.51e-77	226
MS.gene061901.t1	MTR_4g063990	86.577	149	8	3	1	138	1	148	1.51e-77	226
MS.gene061901.t1	MTR_4g063200	86.577	149	8	3	1	138	1	148	1.51e-77	226
MS.gene061901.t1	MTR_4g064020	86.577	149	8	3	1	138	1	148	1.51e-77	226
MS.gene061901.t1	MTR_4g071180	86.577	149	8	3	1	138	1	148	1.41e-76	224
MS.gene061901.t1	MTR_4g070240	86.577	149	8	3	1	138	1	148	1.41e-76	224
MS.gene061901.t1	MTR_4g088025	87.500	144	11	3	1	138	1	143	4.51e-75	220
MS.gene061901.t1	MTR_4g070245	85.235	149	10	3	1	138	1	148	5.68e-75	220
MS.gene061901.t1	MTR_4g063275	83.893	149	12	3	1	138	1	148	2.52e-74	218
MS.gene061901.t1	MTR_4g064983	85.517	145	9	3	1	134	1	144	6.02e-73	218
MS.gene061901.t1	MTR_1g068600	95.370	108	4	1	31	138	30	136	5.60e-65	194
MS.gene061901.t1	MTR_2g084480	60.674	89	35	0	49	137	136	224	2.78e-37	127
MS.gene061901.t1	MTR_8g069990	71.910	89	22	2	9	97	3	88	1.07e-35	119
MS.gene061901.t1	MTR_4g063270	90.196	51	5	0	76	126	1	51	4.41e-27	95.9
MS.gene061901.t1	MTR_7g114580	90.244	41	4	0	98	138	11	51	5.92e-20	77.8
MS.gene061901.t1	MTR_4g032777	39.744	78	47	0	45	122	33	110	7.57e-13	61.6
MS.gene061901.t1	MTR_2g083800	66.071	56	14	3	9	62	11	63	3.72e-11	55.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene061901.t1	AT2G37470	89.855	138	13	1	1	137	1	138	8.42e-79	229
MS.gene061901.t1	AT3G53650	89.855	138	14	0	1	138	1	138	1.22e-77	226
MS.gene061901.t1	AT3G45980	82.781	151	12	3	1	138	1	150	9.13e-75	219
MS.gene061901.t1	AT1G07790	82.550	149	14	2	1	138	1	148	8.27e-74	217
MS.gene061901.t1	AT2G28720	82.237	152	12	3	1	138	1	151	1.86e-73	216
MS.gene061901.t1	AT5G59910	85.926	135	17	1	6	138	16	150	1.53e-71	211
MS.gene061901.t1	AT3G46030	88.372	129	13	1	12	138	17	145	5.88e-71	209
MS.gene061901.t1	AT5G02570	88.550	131	9	2	11	138	5	132	2.03e-67	200
MS.gene061901.t1	AT5G22880	81.119	143	18	2	5	138	3	145	5.66e-66	197
MS.gene061901.t1	AT3G09480	82.114	123	21	1	16	138	5	126	8.28e-64	191
MS.gene061901.t1	AT1G08170	60.000	85	34	0	51	135	151	235	3.06e-37	127

Find 34 sgRNAs with CRISPR-Local

Find 33 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATCAAGAGCTGGTGAATT+TGG	0.235894	7.2:-12027230	None:intergenic
CTCCGCCTTTGGTTTCTTCT+CGG	0.278156	7.2:-12026894	None:intergenic
GCTGTTAGATTGGTTCTTCC+TGG	0.314946	7.2:+12027163	MS.gene061901:CDS
GAGATCTTCTTCTCCGCCTT+TGG	0.325858	7.2:-12026905	None:intergenic
ATTGGTTCTTCCTGGAGAAT+TGG	0.353160	7.2:+12027171	MS.gene061901:CDS
GCCTTTGGTTTCTTCTCGGC+TGG	0.357848	7.2:-12026890	None:intergenic
CATCTTCAAGGTTCTGAAAC+AGG	0.359264	7.2:+12026994	MS.gene061901:CDS
AAACAGGTTCATCCTGATAT+TGG	0.374570	7.2:+12027010	MS.gene061901:CDS
GATTCAAACTGCTGTTAGAT+TGG	0.401031	7.2:+12027153	MS.gene061901:CDS
TTGCTTGAAACACCAATATC+AGG	0.417581	7.2:-12027022	None:intergenic
TGGTGTTTCAAGCAAGGCTA+TGG	0.418611	7.2:+12027030	MS.gene061901:CDS
CGCTCAGGAATCGTCTCGTT+TGG	0.419620	7.2:+12027093	MS.gene061901:CDS
TCGGCTGGAGCTTTCTCTGC+GGG	0.433183	7.2:-12026875	None:intergenic
TGCTGTTTCTGAAGGGACTA+AGG	0.469671	7.2:+12027201	MS.gene061901:CDS
ATACAAGATATACATCTTCA+AGG	0.488494	7.2:+12026982	MS.gene061901:CDS
GCTAAACATGCTGTTTCTGA+AGG	0.504101	7.2:+12027193	MS.gene061901:CDS
TCTGCGGGCTTCTTCTCCGC+GGG	0.504996	7.2:-12026860	None:intergenic
GCATGTTTAGCCAATTCTCC+AGG	0.514866	7.2:-12027181	None:intergenic
ATCTCCCTTGAAGTGATCGT+CGG	0.552358	7.2:-12027133	None:intergenic
CTCTGCGGGCTTCTTCTCCG+CGG	0.552987	7.2:-12026861	None:intergenic
GGTGTTTCAAGCAAGGCTAT+GGG	0.553844	7.2:+12027031	MS.gene061901:CDS
CTCGGCTGGAGCTTTCTCTG+CGG	0.557332	7.2:-12026876	None:intergenic
AGAAGCCGACGATCACTTCA+AGG	0.560392	7.2:+12027128	MS.gene061901:CDS
GAGAAGAAGATCTCAAAAGA+AGG	0.561490	7.2:+12026914	MS.gene061901:CDS
TGATATTGGTGTTTCAAGCA+AGG	0.568075	7.2:+12027024	MS.gene061901:CDS
AGGAATCGTCTCGTTTGGCT+AGG	0.569875	7.2:+12027098	MS.gene061901:CDS
AAAGCAACAAAATCAAGAGC+TGG	0.570887	7.2:-12027239	None:intergenic
TATCTTTGAGAAACTCGCTC+AGG	0.574402	7.2:+12027078	MS.gene061901:CDS
CTAAACATGCTGTTTCTGAA+GGG	0.585084	7.2:+12027194	MS.gene061901:CDS
ATCAAGAATGGCTCCCACAA+AGG	0.630896	7.2:+12026826	None:intergenic
TCCAGCCGAGAAGAAACCAA+AGG	0.637685	7.2:+12026889	MS.gene061901:CDS
AAAGGCAGAAAAGAAACCCG+CGG	0.639354	7.2:+12026844	MS.gene061901:CDS
GAAGCCGACGATCACTTCAA+GGG	0.644853	7.2:+12027129	MS.gene061901:CDS
AGCCGAGAAGAAACCAAAGG+CGG	0.671315	7.2:+12026892	MS.gene061901:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	ATACAAGATATACATCTTCA+AGG	+	chr7.2:12026982-12027001	MS.gene061901:CDS	25.0%
	AAACAGGTTCATCCTGATAT+TGG	+	chr7.2:12027010-12027029	MS.gene061901:CDS	35.0%
	GAGAAGAAGATCTCAAAAGA+AGG	+	chr7.2:12026914-12026933	MS.gene061901:CDS	35.0%
	GATTCAAACTGCTGTTAGAT+TGG	+	chr7.2:12027153-12027172	MS.gene061901:CDS	35.0%
	TTGCTTGAAACACCAATATC+AGG	-	chr7.2:12027025-12027044	None:intergenic	35.0%
!	TGATATTGGTGTTTCAAGCA+AGG	+	chr7.2:12027024-12027043	MS.gene061901:CDS	35.0%
!!	CTAAACATGCTGTTTCTGAA+GGG	+	chr7.2:12027194-12027213	MS.gene061901:CDS	35.0%
!	ATTGGTTCTTCCTGGAGAAT+TGG	+	chr7.2:12027171-12027190	MS.gene061901:CDS	40.0%
!	GGTTTCTTTTCTGCCTTTGT+GGG	-	chr7.2:12026842-12026861	None:intergenic	40.0%
!	TATCTTTGAGAAACTCGCTC+AGG	+	chr7.2:12027078-12027097	MS.gene061901:CDS	40.0%
!!	CATCTTCAAGGTTCTGAAAC+AGG	+	chr7.2:12026994-12027013	MS.gene061901:CDS	40.0%
!!	GCTAAACATGCTGTTTCTGA+AGG	+	chr7.2:12027193-12027212	MS.gene061901:CDS	40.0%
	AAAGGCAGAAAAGAAACCCG+CGG	+	chr7.2:12026844-12026863	MS.gene061901:CDS	45.0%
	ATCTCCCTTGAAGTGATCGT+CGG	-	chr7.2:12027136-12027155	None:intergenic	45.0%
	GCATGTTTAGCCAATTCTCC+AGG	-	chr7.2:12027184-12027203	None:intergenic	45.0%
	GGTGTTTCAAGCAAGGCTAT+GGG	+	chr7.2:12027031-12027050	MS.gene061901:CDS	45.0%
	TGGTGTTTCAAGCAAGGCTA+TGG	+	chr7.2:12027030-12027049	MS.gene061901:CDS	45.0%
!	GCTGTTAGATTGGTTCTTCC+TGG	+	chr7.2:12027163-12027182	MS.gene061901:CDS	45.0%
!	GGGTTTCTTTTCTGCCTTTG+TGG	-	chr7.2:12026843-12026862	None:intergenic	45.0%
!!	TGCTGTTTCTGAAGGGACTA+AGG	+	chr7.2:12027201-12027220	MS.gene061901:CDS	45.0%
	AGAAGCCGACGATCACTTCA+AGG	+	chr7.2:12027128-12027147	MS.gene061901:CDS	50.0%
	AGCCGAGAAGAAACCAAAGG+CGG	+	chr7.2:12026892-12026911	MS.gene061901:CDS	50.0%
	AGGAATCGTCTCGTTTGGCT+AGG	+	chr7.2:12027098-12027117	MS.gene061901:CDS	50.0%
	GAAGCCGACGATCACTTCAA+GGG	+	chr7.2:12027129-12027148	MS.gene061901:CDS	50.0%
	GAGATCTTCTTCTCCGCCTT+TGG	-	chr7.2:12026908-12026927	None:intergenic	50.0%
	TCCAGCCGAGAAGAAACCAA+AGG	+	chr7.2:12026889-12026908	MS.gene061901:CDS	50.0%
!	CTCCGCCTTTGGTTTCTTCT+CGG	-	chr7.2:12026897-12026916	None:intergenic	50.0%
	CGCTCAGGAATCGTCTCGTT+TGG	+	chr7.2:12027093-12027112	MS.gene061901:CDS	55.0%
!	GCCTTTGGTTTCTTCTCGGC+TGG	-	chr7.2:12026893-12026912	None:intergenic	55.0%
	CTCGGCTGGAGCTTTCTCTG+CGG	-	chr7.2:12026879-12026898	None:intergenic	60.0%
	TCGGCTGGAGCTTTCTCTGC+GGG	-	chr7.2:12026878-12026897	None:intergenic	60.0%
	CTCTGCGGGCTTCTTCTCCG+CGG	-	chr7.2:12026864-12026883	None:intergenic	65.0%
	TCTGCGGGCTTCTTCTCCGC+GGG	-	chr7.2:12026863-12026882	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.2	gene	12026833	12027249	12026833	ID=MS.gene061901
chr7.2	mRNA	12026833	12027249	12026833	ID=MS.gene061901.t1;Parent=MS.gene061901
chr7.2	exon	12026833	12027249	12026833	ID=MS.gene061901.t1.exon1;Parent=MS.gene061901.t1
chr7.2	CDS	12026833	12027249	12026833	ID=cds.MS.gene061901.t1;Parent=MS.gene061901.t1

Gene Sequence

>MS.gene061901

ATGGCTCCCACAAAGGCAGAAAAGAAACCCGCGGAGAAGAAGCCCGCAGAGAAAGCTCCAGCCGAGAAGAAACCAAAGGCGGAGAAGAAGATCTCAAAAGAAGGAAGCAGCGACAAGAAGAAAAAGAGAACGAAGAAAAGCGTTGAAACATACAAGATATACATCTTCAAGGTTCTGAAACAGGTTCATCCTGATATTGGTGTTTCAAGCAAGGCTATGGGAATTATGAACAGTTTCATTAACGATATCTTTGAGAAACTCGCTCAGGAATCGTCTCGTTTGGCTAGGTATAACAAGAAGCCGACGATCACTTCAAGGGAGATTCAAACTGCTGTTAGATTGGTTCTTCCTGGAGAATTGGCTAAACATGCTGTTTCTGAAGGGACTAAGGCTGTTACCAAATTCACCAGCTCTTGA

Protein sequence

>MS.gene061901.t1

MAPTKAEKKPAEKKPAEKAPAEKKPKAEKKISKEGSSDKKKKRTKKSVETYKIYIFKVLKQVHPDIGVSSKAMGIMNSFINDIFEKLAQESSRLARYNKKPTITSREIQTAVRLVLPGELAKHAVSEGTKAVTKFTSS