AlfalfaGEDB | MS.gene058976

M. sativa cultivar XinJiangDaYe / MS.gene058976

KEGG: K11252
GO: GO:0000786 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0032991 GO:0032993 GO:0044815 GO:0046982 GO:0046983 GO:0097159 GO:1901363
PFAM: PF00125.27

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene058976.t1	XP_003590511.1	100	136	0	0	1	136	1	136	1.00E-44	189.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene058976.t1	O22582	100.0	97	0	0	40	136	51	147	3.6e-46	185.7

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene058976.t1	G7IBM3	100.0	136	0	0	1	136	1	136	7.4e-45	189.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049255	MS.gene058976	0.821874	3.20E-53	-1.69E-46
MS.gene050689	MS.gene058976	0.834749	2.50E-56	-1.69E-46
MS.gene051003	MS.gene058976	0.828519	8.60E-55	-1.69E-46
MS.gene051343	MS.gene058976	0.802792	4.74E-49	-1.69E-46
MS.gene053058	MS.gene058976	0.846347	2.30E-59	-1.69E-46
MS.gene05517	MS.gene058976	0.80574	1.15E-49	-1.69E-46
MS.gene056181	MS.gene058976	0.812647	3.82E-51	-1.69E-46
MS.gene057392	MS.gene058976	0.805176	1.51E-49	-1.69E-46
MS.gene058976	MS.gene059146	0.819946	8.89E-53	-1.69E-46
MS.gene058976	MS.gene05978	0.812165	4.86E-51	-1.69E-46
MS.gene058976	MS.gene061877	0.811166	8.02E-51	-1.69E-46
MS.gene058976	MS.gene061878	0.86144	1.02E-63	-1.69E-46
MS.gene058976	MS.gene061901	0.819336	1.23E-52	-1.69E-46
MS.gene058976	MS.gene06238	0.80962	1.73E-50	-1.69E-46
MS.gene058976	MS.gene062770	0.858236	9.47E-63	-1.69E-46
MS.gene058976	MS.gene063025	0.820326	7.28E-53	-1.69E-46
MS.gene058976	MS.gene063026	0.805741	1.15E-49	-1.69E-46
MS.gene058976	MS.gene066319	0.804036	2.62E-49	-1.69E-46
MS.gene058976	MS.gene066321	0.805665	1.20E-49	-1.69E-46
MS.gene058976	MS.gene066511	0.802427	5.64E-49	-1.69E-46
MS.gene058976	MS.gene066836	0.813529	2.44E-51	-1.69E-46
MS.gene058976	MS.gene068166	0.819031	1.44E-52	-1.69E-46
MS.gene058976	MS.gene068529	0.86747	1.34E-65	-1.69E-46
MS.gene058976	MS.gene070242	0.823199	1.57E-53	-1.69E-46
MS.gene058976	MS.gene070813	0.805506	1.29E-49	-1.69E-46
MS.gene058976	MS.gene07100	0.815464	9.12E-52	-1.69E-46
MS.gene058976	MS.gene071074	0.835468	1.65E-56	-1.69E-46
MS.gene058976	MS.gene071076	0.825749	3.95E-54	-1.69E-46
MS.gene058976	MS.gene072740	0.848547	5.72E-60	-1.69E-46
MS.gene058976	MS.gene073674	0.844957	5.48E-59	-1.69E-46
MS.gene058976	MS.gene073851	0.930273	2.08E-93	-1.69E-46
MS.gene058976	MS.gene073954	0.809955	1.46E-50	-1.69E-46
MS.gene058976	MS.gene08793	0.820448	6.82E-53	-1.69E-46
MS.gene058976	MS.gene08922	0.813674	2.27E-51	-1.69E-46
MS.gene058976	MS.gene09849	0.822632	2.14E-53	-1.69E-46
MS.gene058976	MS.gene09870	0.831412	1.69E-55	-1.69E-46
MS.gene058976	MS.gene09873	0.809984	1.44E-50	-1.69E-46
MS.gene058976	MS.gene20886	0.814461	1.52E-51	-1.69E-46
MS.gene058976	MS.gene21651	0.839534	1.50E-57	-1.69E-46
MS.gene058976	MS.gene22321	0.857956	1.15E-62	-1.69E-46
MS.gene058976	MS.gene22740	0.829243	5.74E-55	-1.69E-46
MS.gene058976	MS.gene24069	0.905862	2.74E-80	-1.69E-46
MS.gene058976	MS.gene24330	0.809729	1.64E-50	-1.69E-46
MS.gene058976	MS.gene24375	0.818598	1.80E-52	-1.69E-46
MS.gene058976	MS.gene25676	0.801004	1.10E-48	-1.69E-46
MS.gene058976	MS.gene35034	0.815182	1.05E-51	-1.69E-46
MS.gene058976	MS.gene35048	0.803032	4.23E-49	-1.69E-46
MS.gene058976	MS.gene36410	0.801891	7.27E-49	-1.69E-46
MS.gene058976	MS.gene38569	0.834913	2.28E-56	-1.69E-46
MS.gene058976	MS.gene40416	0.800902	1.16E-48	-1.69E-46
MS.gene058976	MS.gene40595	0.805763	1.14E-49	-1.69E-46
MS.gene058976	MS.gene41678	0.801099	1.06E-48	-1.69E-46
MS.gene058976	MS.gene42302	0.820127	8.08E-53	-1.69E-46
MS.gene058976	MS.gene44260	0.803261	3.79E-49	-1.69E-46
MS.gene058976	MS.gene49426	0.801418	9.09E-49	-1.69E-46
MS.gene058976	MS.gene52837	0.856528	3.03E-62	-1.69E-46
MS.gene058976	MS.gene54158	0.817705	2.87E-52	-1.69E-46
MS.gene058976	MS.gene55530	0.809648	1.71E-50	-1.69E-46
MS.gene058976	MS.gene55705	0.807251	5.54E-50	-1.69E-46
MS.gene058976	MS.gene56414	0.800269	1.56E-48	-1.69E-46
MS.gene058976	MS.gene56451	0.810498	1.12E-50	-1.69E-46
MS.gene058976	MS.gene56656	0.804998	1.65E-49	-1.69E-46
MS.gene058976	MS.gene58098	0.81543	9.28E-52	-1.69E-46
MS.gene058976	MS.gene58134	0.810778	9.73E-51	-1.69E-46
MS.gene058976	MS.gene59051	0.814516	1.48E-51	-1.69E-46
MS.gene058976	MS.gene61282	0.813425	2.58E-51	-1.69E-46
MS.gene058976	MS.gene62430	0.815648	8.30E-52	-1.69E-46
MS.gene058976	MS.gene64243	0.804633	1.97E-49	-1.69E-46
MS.gene058976	MS.gene64806	0.801219	9.98E-49	-1.69E-46
MS.gene058976	MS.gene67099	0.804463	2.13E-49	-1.69E-46
MS.gene058976	MS.gene68136	0.827072	1.91E-54	-1.69E-46
MS.gene058976	MS.gene68735	0.843326	1.50E-58	-1.69E-46
MS.gene058976	MS.gene69251	0.806779	6.97E-50	-1.69E-46
MS.gene058976	MS.gene70282	0.814551	1.45E-51	-1.69E-46
MS.gene058976	MS.gene71215	0.83306	6.62E-56	-1.69E-46
MS.gene058976	MS.gene71994	0.83447	2.94E-56	-1.69E-46
MS.gene058976	MS.gene72353	0.810122	1.35E-50	-1.69E-46
MS.gene058976	MS.gene75605	0.856965	2.25E-62	-1.69E-46
MS.gene058976	MS.gene82828	0.802427	5.64E-49	-1.69E-46
MS.gene058976	MS.gene85610	0.800675	1.29E-48	-1.69E-46
MS.gene058976	MS.gene88680	0.82054	6.50E-53	-1.69E-46
MS.gene058976	MS.gene89677	0.827084	1.90E-54	-1.69E-46
MS.gene058976	MS.gene92390	0.814328	1.63E-51	-1.69E-46
MS.gene058976	MS.gene95235	0.817529	3.15E-52	-1.69E-46
MS.gene058976	MS.gene96191	0.820754	5.80E-53	-1.69E-46
MS.gene058976	MS.gene99509	0.804316	2.29E-49	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene025425	MS.gene058976	PPI
MS.gene058976	MS.gene84498	PPI
MS.gene066348	MS.gene058976	PPI
MS.gene24295	MS.gene058976	PPI
MS.gene058976	MS.gene65249	PPI
MS.gene058976	MS.gene08465	PPI
MS.gene75219	MS.gene058976	PPI
MS.gene058976	MS.gene99525	PPI
MS.gene025426	MS.gene058976	PPI
MS.gene058976	MS.gene64653	PPI
MS.gene035396	MS.gene058976	PPI
MS.gene058976	MS.gene041623	PPI
MS.gene049824	MS.gene058976	PPI
MS.gene058976	MS.gene025035	PPI
MS.gene058976	MS.gene63143	PPI
MS.gene058976	MS.gene81051	PPI
MS.gene004126	MS.gene058976	PPI
MS.gene058976	MS.gene63953	PPI
MS.gene058976	MS.gene066300	PPI
MS.gene004127	MS.gene058976	PPI
MS.gene058976	MS.gene27326	PPI
MS.gene058976	MS.gene066301	PPI
MS.gene65007	MS.gene058976	PPI
MS.gene058976	MS.gene049822	PPI
MS.gene26950	MS.gene058976	PPI
MS.gene058976	MS.gene051008	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene058976.t1	MTR_1g068600	99.265	136	1	0	1	136	1	136	1.04e-93	266
MS.gene058976.t1	MTR_4g063240	85.034	147	11	2	1	136	1	147	1.77e-81	236
MS.gene058976.t1	MTR_4g063220	85.811	148	9	2	1	136	1	148	4.40e-78	228
MS.gene058976.t1	MTR_4g063990	85.811	148	9	2	1	136	1	148	4.40e-78	228
MS.gene058976.t1	MTR_4g063200	85.811	148	9	2	1	136	1	148	4.40e-78	228
MS.gene058976.t1	MTR_4g064020	85.811	148	9	2	1	136	1	148	4.40e-78	228
MS.gene058976.t1	MTR_1g090780	91.304	138	9	2	1	136	1	137	5.11e-78	227
MS.gene058976.t1	MTR_1g492910	91.304	138	9	2	1	136	1	137	5.89e-78	227
MS.gene058976.t1	MTR_4g071180	85.811	148	9	2	1	136	1	148	4.30e-77	225
MS.gene058976.t1	MTR_4g070240	85.811	148	9	2	1	136	1	148	4.30e-77	225
MS.gene058976.t1	MTR_4g088025	86.713	143	12	2	1	136	1	143	9.26e-76	221
MS.gene058976.t1	MTR_4g070245	84.459	148	11	2	1	136	1	148	1.54e-75	221
MS.gene058976.t1	MTR_4g063275	83.108	148	13	2	1	136	1	148	4.86e-75	220
MS.gene058976.t1	MTR_4g064983	84.722	144	10	2	1	132	1	144	2.53e-73	219
MS.gene058976.t1	MTR_7g099960	95.370	108	4	1	30	136	31	138	2.89e-64	192
MS.gene058976.t1	MTR_2g084480	62.921	89	33	0	47	135	136	224	9.85e-38	128
MS.gene058976.t1	MTR_8g069990	71.591	88	23	2	8	95	3	88	6.01e-35	117
MS.gene058976.t1	MTR_4g063270	90.196	51	5	0	74	124	1	51	5.77e-27	95.5
MS.gene058976.t1	MTR_7g114580	90.244	41	4	0	96	136	11	51	7.05e-20	77.4
MS.gene058976.t1	MTR_4g032777	39.744	78	47	0	43	120	33	110	1.43e-12	60.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene058976.t1	AT3G45980	83.333	150	11	2	1	136	1	150	6.00e-77	225
MS.gene058976.t1	AT2G37470	88.406	138	13	2	1	135	1	138	7.78e-76	221
MS.gene058976.t1	AT1G07790	83.784	148	12	2	1	136	1	148	2.33e-75	221
MS.gene058976.t1	AT2G28720	83.444	151	10	2	1	136	1	151	2.26e-74	218
MS.gene058976.t1	AT3G53650	87.681	138	15	2	1	136	1	138	1.55e-73	216
MS.gene058976.t1	AT5G59910	87.407	135	14	1	5	136	16	150	6.19e-73	214
MS.gene058976.t1	AT3G46030	88.372	129	12	1	11	136	17	145	1.85e-71	211
MS.gene058976.t1	AT5G02570	83.571	140	11	2	1	136	1	132	1.41e-68	203
MS.gene058976.t1	AT3G09480	80.147	136	17	1	1	136	1	126	1.23e-65	195
MS.gene058976.t1	AT5G22880	81.119	143	17	2	4	136	3	145	2.62e-65	195
MS.gene058976.t1	AT1G08170	62.353	85	32	0	49	133	151	235	8.86e-38	128

Find 33 sgRNAs with CRISPR-Local

Find 33 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTTGAAGATGTAAATCTTAT+AGG	0.133504	1.2:-51444135	None:intergenic
TAATTAAGAGCTAGTAAATT+TGG	0.233127	1.2:-51444384	None:intergenic
TTCTTTCGAGATCTTCTTCT+CGG	0.293193	1.2:-51444069	None:intergenic
GCTGTGCGTTTGGTTCTTCC+TGG	0.321985	1.2:+51444317	MS.gene058976:CDS
TTTGAAGAAATACCTATATC+AGG	0.334896	1.2:-51444176	None:intergenic
CATCTTCAAGGTTCTCAAAC+AGG	0.361477	1.2:+51444148	MS.gene058976:CDS
GAGATCTTCTTCTCGGCTCT+TGG	0.362586	1.2:-51444062	None:intergenic
CTATAAGATTTACATCTTCA+AGG	0.370441	1.2:+51444136	MS.gene058976:CDS
GATTCAAACCGCTGTGCGTT+TGG	0.390239	1.2:+51444307	MS.gene058976:CDS
TTTGGTTCTTCCTGGTGAAT+TGG	0.398260	1.2:+51444325	MS.gene058976:CDS
TTTCTCTGCCTTGGGAGCCA+TGG	0.402280	1.2:-51443991	None:intergenic
AAACAGGTTCATCCTGATAT+AGG	0.421612	1.2:+51444164	MS.gene058976:CDS
AGGTATTTCTTCAAAAGCTA+TGG	0.429032	1.2:+51444184	MS.gene058976:CDS
AGTAAATTTGGTAACAGCCT+TGG	0.444512	1.2:-51444372	None:intergenic
CTTGTTGTAACGAGCCAATC+TGG	0.445538	1.2:-51444261	None:intergenic
TCCGCGGGTTTCTTCTCAGC+GGG	0.464857	1.2:-51444017	None:intergenic
TCTGCTGGTGATTTCTCCGC+GGG	0.470002	1.2:-51444032	None:intergenic
CGCTCAGGAAGCTTCCAGAT+TGG	0.475450	1.2:+51444247	MS.gene058976:CDS
GCATGTTTCGCCAATTCACC+AGG	0.516345	1.2:-51444335	None:intergenic
GGTATTTCTTCAAAAGCTAT+GGG	0.517184	1.2:+51444185	MS.gene058976:CDS
GCGAAACATGCTGTTTCTGA+AGG	0.530754	1.2:+51444347	MS.gene058976:CDS
ATTCAGATCCATGGCTCCCA+AGG	0.541897	1.2:+51443983	None:intergenic
GAAACCGACGATTACGTCAC+GGG	0.556515	1.2:+51444283	MS.gene058976:CDS
CTCTGCTGGTGATTTCTCCG+CGG	0.560516	1.2:-51444033	None:intergenic
GAGAAGAAGATCTCGAAAGA+AGG	0.564931	1.2:+51444071	MS.gene058976:CDS
TATCTTTGAGAAACTCGCTC+AGG	0.586744	1.2:+51444232	MS.gene058976:CDS
ATCTCCCGTGACGTAATCGT+CGG	0.590908	1.2:-51444287	None:intergenic
TGCTGTTTCTGAAGGAACCA+AGG	0.603820	1.2:+51444355	MS.gene058976:CDS
CTCCGCGGGTTTCTTCTCAG+CGG	0.628471	1.2:-51444018	None:intergenic
AAGAAGATCTCGAAAGAAGG+AGG	0.628797	1.2:+51444074	MS.gene058976:CDS
AGAAACCGACGATTACGTCA+CGG	0.672005	1.2:+51444282	MS.gene058976:CDS
ACCCGCTGAGAAGAAACCCG+CGG	0.690098	1.2:+51444016	MS.gene058976:CDS
AGGAAGAACCAAACGCACAG+CGG	0.757762	1.2:-51444315	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	CTATAAGATTTACATCTTCA+AGG	+	chr1.2:51444136-51444155	MS.gene058976:CDS	25.0%
!	CTTGAAGATGTAAATCTTAT+AGG	-	chr1.2:51444138-51444157	None:intergenic	25.0%
!	TTTGAAGAAATACCTATATC+AGG	-	chr1.2:51444179-51444198	None:intergenic	25.0%
	AGGTATTTCTTCAAAAGCTA+TGG	+	chr1.2:51444184-51444203	MS.gene058976:CDS	30.0%
	GGTATTTCTTCAAAAGCTAT+GGG	+	chr1.2:51444185-51444204	MS.gene058976:CDS	30.0%
	AAACAGGTTCATCCTGATAT+AGG	+	chr1.2:51444164-51444183	MS.gene058976:CDS	35.0%
	TTCTTTCGAGATCTTCTTCT+CGG	-	chr1.2:51444072-51444091	None:intergenic	35.0%
!	AGTAAATTTGGTAACAGCCT+TGG	-	chr1.2:51444375-51444394	None:intergenic	35.0%
	AAGAAGATCTCGAAAGAAGG+AGG	+	chr1.2:51444074-51444093	MS.gene058976:CDS	40.0%
	CATCTTCAAGGTTCTCAAAC+AGG	+	chr1.2:51444148-51444167	MS.gene058976:CDS	40.0%
	GAGAAGAAGATCTCGAAAGA+AGG	+	chr1.2:51444071-51444090	MS.gene058976:CDS	40.0%
!	TATCTTTGAGAAACTCGCTC+AGG	+	chr1.2:51444232-51444251	MS.gene058976:CDS	40.0%
!!	TTTGGTTCTTCCTGGTGAAT+TGG	+	chr1.2:51444325-51444344	MS.gene058976:CDS	40.0%
	AGAAACCGACGATTACGTCA+CGG	+	chr1.2:51444282-51444301	MS.gene058976:CDS	45.0%
!	CTTGTTGTAACGAGCCAATC+TGG	-	chr1.2:51444264-51444283	None:intergenic	45.0%
!!	GCGAAACATGCTGTTTCTGA+AGG	+	chr1.2:51444347-51444366	MS.gene058976:CDS	45.0%
!!	TGCTGTTTCTGAAGGAACCA+AGG	+	chr1.2:51444355-51444374	MS.gene058976:CDS	45.0%
	AGGAAGAACCAAACGCACAG+CGG	-	chr1.2:51444318-51444337	None:intergenic	50.0%
	ATCTCCCGTGACGTAATCGT+CGG	-	chr1.2:51444290-51444309	None:intergenic	50.0%
	GAAACCGACGATTACGTCAC+GGG	+	chr1.2:51444283-51444302	MS.gene058976:CDS	50.0%
	GAGATCTTCTTCTCGGCTCT+TGG	-	chr1.2:51444065-51444084	None:intergenic	50.0%
	GATTCAAACCGCTGTGCGTT+TGG	+	chr1.2:51444307-51444326	MS.gene058976:CDS	50.0%
	GCATGTTTCGCCAATTCACC+AGG	-	chr1.2:51444338-51444357	None:intergenic	50.0%
!!	GCTCTTGGCTTTTTCTCTGC+TGG	-	chr1.2:51444050-51444069	None:intergenic	50.0%
!!!	AGCGGGTTTTTTCTCTGCCT+TGG	-	chr1.2:51444003-51444022	None:intergenic	50.0%
!!!	GCGGGTTTTTTCTCTGCCTT+GGG	-	chr1.2:51444002-51444021	None:intergenic	50.0%
	CGCTCAGGAAGCTTCCAGAT+TGG	+	chr1.2:51444247-51444266	MS.gene058976:CDS	55.0%
	CTCTGCTGGTGATTTCTCCG+CGG	-	chr1.2:51444036-51444055	None:intergenic	55.0%
	TCTGCTGGTGATTTCTCCGC+GGG	-	chr1.2:51444035-51444054	None:intergenic	55.0%
!!	GCTGTGCGTTTGGTTCTTCC+TGG	+	chr1.2:51444317-51444336	MS.gene058976:CDS	55.0%
	ACCCGCTGAGAAGAAACCCG+CGG	+	chr1.2:51444016-51444035	MS.gene058976:CDS	60.0%
	CTCCGCGGGTTTCTTCTCAG+CGG	-	chr1.2:51444021-51444040	None:intergenic	60.0%
	TCCGCGGGTTTCTTCTCAGC+GGG	-	chr1.2:51444020-51444039	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.2	gene	51443993	51444403	51443993	ID=MS.gene058976
chr1.2	mRNA	51443993	51444403	51443993	ID=MS.gene058976.t1;Parent=MS.gene058976
chr1.2	exon	51443993	51444403	51443993	ID=MS.gene058976.t1.exon1;Parent=MS.gene058976.t1
chr1.2	CDS	51443993	51444403	51443993	ID=cds.MS.gene058976.t1;Parent=MS.gene058976.t1

Gene Sequence

>MS.gene058976

ATGGCTCCCAAGGCAGAGAAAAAACCCGCTGAGAAGAAACCCGCGGAGAAATCACCAGCAGAGAAAAAGCCAAGAGCCGAGAAGAAGATCTCGAAAGAAGGAGGCGATAAGAAGAAGAAGCGCGTGAAGAAGAGCGTTGAAACCTATAAGATTTACATCTTCAAGGTTCTCAAACAGGTTCATCCTGATATAGGTATTTCTTCAAAAGCTATGGGAATTATGAACAGTTTCATCAATGATATCTTTGAGAAACTCGCTCAGGAAGCTTCCAGATTGGCTCGTTACAACAAGAAACCGACGATTACGTCACGGGAGATTCAAACCGCTGTGCGTTTGGTTCTTCCTGGTGAATTGGCGAAACATGCTGTTTCTGAAGGAACCAAGGCTGTTACCAAATTTACTAGCTCTTAA

Protein sequence

>MS.gene058976.t1

MAPKAEKKPAEKKPAEKSPAEKKPRAEKKISKEGGDKKKKRVKKSVETYKIYIFKVLKQVHPDIGISSKAMGIMNSFINDIFEKLAQEASRLARYNKKPTITSREIQTAVRLVLPGELAKHAVSEGTKAVTKFTSS