Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064038.t1 | XP_003620880.2 | 100 | 148 | 0 | 0 | 1 | 148 | 236 | 383 | 6.90E-79 | 303.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064038.t1 | A2Z0C0 | 80.4 | 148 | 29 | 0 | 1 | 148 | 240 | 387 | 1.2e-66 | 253.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064038.t1 | G7KJ80 | 100.0 | 148 | 0 | 0 | 1 | 148 | 236 | 383 | 5.0e-79 | 303.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene05188 | MS.gene064038 | 0.832104 | 1.14E-55 | -1.69E-46 |
| MS.gene051975 | MS.gene064038 | 0.807459 | 5.00E-50 | -1.69E-46 |
| MS.gene052717 | MS.gene064038 | 0.807345 | 5.29E-50 | -1.69E-46 |
| MS.gene053963 | MS.gene064038 | 0.800559 | 1.36E-48 | -1.69E-46 |
| MS.gene055603 | MS.gene064038 | 0.803316 | 3.69E-49 | -1.69E-46 |
| MS.gene055808 | MS.gene064038 | 0.815687 | 8.14E-52 | -1.69E-46 |
| MS.gene056091 | MS.gene064038 | 0.816182 | 6.31E-52 | -1.69E-46 |
| MS.gene056403 | MS.gene064038 | 0.852479 | 4.49E-61 | -1.69E-46 |
| MS.gene056605 | MS.gene064038 | 0.857378 | 1.70E-62 | -1.69E-46 |
| MS.gene05689 | MS.gene064038 | 0.820879 | 5.43E-53 | -1.69E-46 |
| MS.gene056986 | MS.gene064038 | 0.820969 | 5.18E-53 | -1.69E-46 |
| MS.gene058908 | MS.gene064038 | 0.809789 | 1.59E-50 | -1.69E-46 |
| MS.gene058911 | MS.gene064038 | 0.817072 | 3.99E-52 | -1.69E-46 |
| MS.gene059901 | MS.gene064038 | -0.835129 | 2.01E-56 | -1.69E-46 |
| MS.gene059935 | MS.gene064038 | 0.853982 | 1.67E-61 | -1.69E-46 |
| MS.gene06011 | MS.gene064038 | 0.836131 | 1.12E-56 | -1.69E-46 |
| MS.gene060429 | MS.gene064038 | 0.801614 | 8.29E-49 | -1.69E-46 |
| MS.gene060830 | MS.gene064038 | 0.875349 | 3.31E-68 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064038.t1 | MTR_6g091770 | 100.000 | 148 | 0 | 0 | 1 | 148 | 236 | 383 | 3.66e-109 | 315 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064038.t1 | AT4G29510 | 80.405 | 148 | 29 | 0 | 1 | 148 | 243 | 390 | 2.61e-89 | 265 |
| MS.gene064038.t1 | AT2G19670 | 78.378 | 148 | 32 | 0 | 1 | 148 | 219 | 366 | 3.11e-85 | 254 |
Find 0 sgRNAs with CRISPR-Local
Find 69 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTCTTAAAAAAAGAAAATT+GGG | + | 42523:1028-1047 | MS.gene064038:intron | 10.0% |
| !! | TATTCTTAAAAAAAGAAAAT+TGG | + | 42523:1027-1046 | MS.gene064038:intron | 10.0% |
| !! | AAATGATATTATAACAAAGA+CGG | - | 42523:1323-1342 | None:intergenic | 15.0% |
| !! | TTCTTAAAAAAAGAAAATTG+GGG | + | 42523:1029-1048 | MS.gene064038:intron | 15.0% |
| !!! | ATGAAATTTTTCTTTTCATT+TGG | + | 42523:678-697 | MS.gene064038:intron | 15.0% |
| !!! | ATTCATTTTTATCATTTTAG+TGG | + | 42523:1358-1377 | MS.gene064038:intron | 15.0% |
| !!! | TTGTTATAATATCATTTTAG+TGG | + | 42523:1326-1345 | MS.gene064038:intron | 15.0% |
| !!! | AATTTTAAAACTGTTCCAAA+TGG | + | 42523:1089-1108 | MS.gene064038:intron | 20.0% |
| !!! | ATTTTAAAACTGTTCCAAAT+GGG | + | 42523:1090-1109 | MS.gene064038:intron | 20.0% |
| !!! | CATGTATTTTTTCTGAAAAT+CGG | + | 42523:1244-1263 | MS.gene064038:intron | 20.0% |
| ! | TAAAAAAAGAAAATTGGGGA+GGG | + | 42523:1033-1052 | MS.gene064038:intron | 25.0% |
| ! | TGTGTACATATATATTTCCA+GGG | + | 42523:1399-1418 | MS.gene064038:intron | 25.0% |
| ! | TTAAAAAAAGAAAATTGGGG+AGG | + | 42523:1032-1051 | MS.gene064038:intron | 25.0% |
| ! | TTGTGTACATATATATTTCC+AGG | + | 42523:1398-1417 | MS.gene064038:intron | 25.0% |
| !! | ACAAAGTGTCATAAATTGAT+GGG | + | 42523:882-901 | MS.gene064038:CDS | 25.0% |
| !! | TACAAAGTGTCATAAATTGA+TGG | + | 42523:881-900 | MS.gene064038:CDS | 25.0% |
| AAAAAAAGAAAATTGGGGAG+GGG | + | 42523:1034-1053 | MS.gene064038:intron | 30.0% | |
| ATGCTCAAGTATTCATTGAA+TGG | + | 42523:1557-1576 | MS.gene064038:CDS | 30.0% | |
| CCACAAAAACTAAAGTAGAA+AGG | - | 42523:926-945 | None:intergenic | 30.0% | |
| GACATAATTCAAAACCCATT+TGG | - | 42523:1107-1126 | None:intergenic | 30.0% | |
| GGGATTATTATCAATTAGTG+TGG | + | 42523:1056-1075 | MS.gene064038:intron | 30.0% | |
| TGTATGAAACATACAATGCA+AGG | - | 42523:643-662 | None:intergenic | 30.0% | |
| TTAACTGAACTGAAAAGAAC+TGG | + | 42523:1198-1217 | MS.gene064038:intron | 30.0% | |
| !! | GTTTTGAATTATGTCACTTG+AGG | + | 42523:1112-1131 | MS.gene064038:intron | 30.0% |
| !!! | CCTTTCTACTTTAGTTTTTG+TGG | + | 42523:923-942 | MS.gene064038:intron | 30.0% |
| AAAAAAGAAAATTGGGGAGG+GGG | + | 42523:1035-1054 | MS.gene064038:intron | 35.0% | |
| CCCAAATAAAAAGAATCCTC+GGG | + | 42523:1523-1542 | MS.gene064038:CDS | 35.0% | |
| CCCCAAATAAAAAGAATCCT+CGG | + | 42523:1522-1541 | MS.gene064038:CDS | 35.0% | |
| GAAACAAACAGTCCTATATC+TGG | + | 42523:1445-1464 | MS.gene064038:CDS | 35.0% | |
| GACATCTCAAAAATGTCTTC+TGG | + | 42523:771-790 | MS.gene064038:CDS | 35.0% | |
| TCAAGACATCTTCCAGATAT+AGG | - | 42523:1460-1479 | None:intergenic | 35.0% | |
| TCAAGTATTCATTGAATGGC+AGG | + | 42523:1561-1580 | MS.gene064038:CDS | 35.0% | |
| ! | TCCCGAGGATTCTTTTTATT+TGG | - | 42523:1527-1546 | None:intergenic | 35.0% |
| !! | CATAAAAGCATCATCAGAAC+TGG | - | 42523:611-630 | None:intergenic | 35.0% |
| !! | CCCGAGGATTCTTTTTATTT+GGG | - | 42523:1526-1545 | None:intergenic | 35.0% |
| !! | CCGAGGATTCTTTTTATTTG+GGG | - | 42523:1525-1544 | None:intergenic | 35.0% |
| !!! | AGACATTTTTGAGATGTCCA+TGG | - | 42523:769-788 | None:intergenic | 35.0% |
| AAAAAGAAAATTGGGGAGGG+GGG | + | 42523:1036-1055 | MS.gene064038:intron | 40.0% | |
| AACAAAGACGGAACCACAAA+TGG | - | 42523:1311-1330 | None:intergenic | 40.0% | |
| ACAAAGACGGAACCACAAAT+GGG | - | 42523:1310-1329 | None:intergenic | 40.0% | |
| AGATGTCTTGACCATTTGTG+AGG | + | 42523:1469-1488 | MS.gene064038:CDS | 40.0% | |
| AGCATTATATCAACATCCCG+AGG | - | 42523:1542-1561 | None:intergenic | 40.0% | |
| ATGTCTTGACCATTTGTGAG+GGG | + | 42523:1471-1490 | MS.gene064038:CDS | 40.0% | |
| GATGTCTTGACCATTTGTGA+GGG | + | 42523:1470-1489 | MS.gene064038:CDS | 40.0% | |
| GTTCATCTTTGTTCAGTCCA+TGG | + | 42523:749-768 | MS.gene064038:intron | 40.0% | |
| TTCAACAGTCTTCCCATTTG+TGG | + | 42523:1295-1314 | MS.gene064038:intron | 40.0% | |
| TTGTGGTCTGCTTTGTCAAT+AGG | + | 42523:940-959 | MS.gene064038:intron | 40.0% | |
| !! | AAATTGATGGGCTTCTCAAC+AGG | + | 42523:894-913 | MS.gene064038:CDS | 40.0% |
| !! | TATCATTTTAGTGGCGAGTG+TGG | + | 42523:1335-1354 | MS.gene064038:intron | 40.0% |
| AACGAACTGTCAGCTACTCA+AGG | + | 42523:575-594 | MS.gene064038:CDS | 45.0% | |
| AACTGTCAGCTACTCAAGGT+CGG | + | 42523:579-598 | MS.gene064038:intron | 45.0% | |
| AATTGTCTCCCCCTCACAAA+TGG | - | 42523:1483-1502 | None:intergenic | 45.0% | |
| AGTTCGTTGCGATCTGATTC+TGG | - | 42523:563-582 | None:intergenic | 45.0% | |
| CACATGATGTGTCAGCTCAA+TGG | - | 42523:1157-1176 | None:intergenic | 45.0% | |
| CATCAAGAAGCAAGCCTTGA+TGG | + | 42523:515-534 | MS.gene064038:CDS | 45.0% | |
| CGAGCAGCAACAAGCTTAAA+AGG | - | 42523:816-835 | None:intergenic | 45.0% | |
| GGTCTGCTTTGTCAATAGGA+AGG | + | 42523:944-963 | MS.gene064038:intron | 45.0% | |
| TGGTCAACTGTGTCAACAAG+AGG | - | 42523:543-562 | None:intergenic | 45.0% | |
| TGTCTTGACCATTTGTGAGG+GGG | + | 42523:1472-1491 | MS.gene064038:CDS | 45.0% | |
| ! | AATTGTAGGGAGCATGACTG+TGG | + | 42523:1499-1518 | MS.gene064038:CDS | 45.0% |
| CAAGATCGCGGAGTACACAT+TGG | + | 42523:1423-1442 | MS.gene064038:CDS | 50.0% | |
| CAATGTGTACTCCGCGATCT+TGG | - | 42523:1425-1444 | None:intergenic | 50.0% | |
| GTCAACAAGAGGCTCCATCA+AGG | - | 42523:532-551 | None:intergenic | 50.0% | |
| GTGAGGGGGAGACAATTGTA+GGG | + | 42523:1486-1505 | MS.gene064038:CDS | 50.0% | |
| TATTTCCAGGGCCAAGATCG+CGG | + | 42523:1411-1430 | MS.gene064038:intron | 50.0% | |
| TGTGAGGGGGAGACAATTGT+AGG | + | 42523:1485-1504 | MS.gene064038:CDS | 50.0% | |
| CAGGCGATGCAATGCTTCGA+GGG | + | 42523:1580-1599 | MS.gene064038:CDS | 55.0% | |
| GCAGGCGATGCAATGCTTCG+AGG | + | 42523:1579-1598 | MS.gene064038:CDS | 60.0% | |
| GTACTCCGCGATCTTGGCCC+TGG | - | 42523:1419-1438 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 42523 | gene | 507 | 1625 | 507 | ID=MS.gene064038 |
| 42523 | mRNA | 507 | 1625 | 507 | ID=MS.gene064038.t1;Parent=MS.gene064038 |
| 42523 | exon | 507 | 596 | 507 | ID=MS.gene064038.t1.exon1;Parent=MS.gene064038.t1 |
| 42523 | CDS | 507 | 596 | 507 | ID=cds.MS.gene064038.t1;Parent=MS.gene064038.t1 |
| 42523 | exon | 765 | 915 | 765 | ID=MS.gene064038.t1.exon2;Parent=MS.gene064038.t1 |
| 42523 | CDS | 765 | 915 | 765 | ID=cds.MS.gene064038.t1;Parent=MS.gene064038.t1 |
| 42523 | exon | 1420 | 1625 | 1420 | ID=MS.gene064038.t1.exon3;Parent=MS.gene064038.t1 |
| 42523 | CDS | 1420 | 1625 | 1420 | ID=cds.MS.gene064038.t1;Parent=MS.gene064038.t1 |
| Gene Sequence |
| Protein sequence |
