Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064674.t1 | XP_013462130.1 | 89.1 | 138 | 9 | 1 | 1 | 132 | 1 | 138 | 4.00E-62 | 247.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064674.t1 | I3SPV4 | 89.1 | 138 | 9 | 1 | 1 | 132 | 1 | 138 | 2.9e-62 | 247.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050670 | MS.gene064674 | -0.830201 | 3.35E-55 | -1.69E-46 |
| MS.gene051525 | MS.gene064674 | -0.823471 | 1.36E-53 | -1.69E-46 |
| MS.gene052011 | MS.gene064674 | 0.855692 | 5.32E-62 | -1.69E-46 |
| MS.gene052128 | MS.gene064674 | 0.807569 | 4.74E-50 | -1.69E-46 |
| MS.gene052253 | MS.gene064674 | -0.821126 | 4.77E-53 | -1.69E-46 |
| MS.gene053873 | MS.gene064674 | -0.825943 | 3.56E-54 | -1.69E-46 |
| MS.gene054183 | MS.gene064674 | -0.841516 | 4.53E-58 | -1.69E-46 |
| MS.gene054920 | MS.gene064674 | -0.813035 | 3.14E-51 | -1.69E-46 |
| MS.gene055369 | MS.gene064674 | -0.810432 | 1.16E-50 | -1.69E-46 |
| MS.gene055909 | MS.gene064674 | -0.815944 | 7.13E-52 | -1.69E-46 |
| MS.gene055911 | MS.gene064674 | -0.823582 | 1.28E-53 | -1.69E-46 |
| MS.gene056511 | MS.gene064674 | -0.801865 | 7.36E-49 | -1.69E-46 |
| MS.gene056514 | MS.gene064674 | -0.837098 | 6.35E-57 | -1.69E-46 |
| MS.gene057476 | MS.gene064674 | -0.842137 | 3.11E-58 | -1.69E-46 |
| MS.gene057749 | MS.gene064674 | -0.815927 | 7.19E-52 | -1.69E-46 |
| MS.gene057823 | MS.gene064674 | -0.832823 | 7.59E-56 | -1.69E-46 |
| MS.gene057888 | MS.gene064674 | -0.827571 | 1.45E-54 | -1.69E-46 |
| MS.gene058218 | MS.gene064674 | 0.804751 | 1.86E-49 | -1.69E-46 |
| MS.gene059082 | MS.gene064674 | -0.802642 | 5.09E-49 | -1.69E-46 |
| MS.gene059420 | MS.gene064674 | -0.865523 | 5.55E-65 | -1.69E-46 |
| MS.gene059462 | MS.gene064674 | -0.814507 | 1.49E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064674.t1 | MTR_3g111970 | 89.130 | 138 | 9 | 1 | 1 | 132 | 1 | 138 | 2.32e-86 | 248 |
| MS.gene064674.t1 | MTR_3g111970 | 87.826 | 115 | 8 | 1 | 1 | 109 | 1 | 115 | 4.86e-69 | 203 |
| MS.gene064674.t1 | MTR_1g022395 | 57.042 | 142 | 50 | 4 | 1 | 131 | 1 | 142 | 3.43e-48 | 152 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064674.t1 | AT1G19530 | 44.628 | 121 | 57 | 4 | 1 | 120 | 1 | 112 | 7.92e-21 | 81.6 |
| MS.gene064674.t1 | AT1G19530 | 44.628 | 121 | 57 | 4 | 1 | 120 | 1 | 112 | 7.92e-21 | 81.6 |
Find 39 sgRNAs with CRISPR-Local
Find 57 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATGAAGAAATTGATGCTTTC+TGG | 0.114327 | 3.4:+97013861 | MS.gene064674:CDS |
| ATTAATTAAGTACCTGATTT+AGG | 0.181404 | 3.4:-97013733 | None:intergenic |
| CTGAATAGTCCTTGATAAAT+TGG | 0.192807 | 3.4:-97013927 | None:intergenic |
| CAATTTATCAAGGACTATTC+AGG | 0.271260 | 3.4:+97013928 | MS.gene064674:CDS |
| TCAATCAATGGGTTCTCTTA+TGG | 0.329426 | 3.4:+97013684 | None:intergenic |
| CACGATATCCTCATATTTGC+CGG | 0.330137 | 3.4:-97014070 | None:intergenic |
| GAAAGCATCAATTTCTTCAT+TGG | 0.336879 | 3.4:-97013858 | None:intergenic |
| CGCCCCATGAAGAAATCATT+TGG | 0.393233 | 3.4:+97014102 | MS.gene064674:CDS |
| GCAGGTGGACAAAGAGCAAC+TGG | 0.398226 | 3.4:+97014364 | MS.gene064674:intron |
| TTGGTAGGAGCTTCCATCAC+TGG | 0.398603 | 3.4:-97014405 | None:intergenic |
| CAGCTTCACTTGAAAGGAAT+CGG | 0.401823 | 3.4:+97013828 | MS.gene064674:intron |
| CATGCCAAATGATTTCTTCA+TGG | 0.402591 | 3.4:-97014106 | None:intergenic |
| TTCTTCATGGGGCGGGCCAA+GGG | 0.427383 | 3.4:-97014093 | None:intergenic |
| ATGCCAAATGATTTCTTCAT+GGG | 0.446330 | 3.4:-97014105 | None:intergenic |
| TTTCTTCATGGGGCGGGCCA+AGG | 0.451036 | 3.4:-97014094 | None:intergenic |
| ATAAATTGGAAATAACTTTG+AGG | 0.453495 | 3.4:-97013913 | None:intergenic |
| CACACAACTGATCCTAAATC+AGG | 0.475780 | 3.4:+97013721 | MS.gene064674:CDS |
| AAGTTATTTCCAATTTATCA+AGG | 0.477495 | 3.4:+97013918 | MS.gene064674:CDS |
| AAATGATTTCTTCATGGGGC+GGG | 0.491715 | 3.4:-97014100 | None:intergenic |
| TGCTTTCTGGAGATCAAAGA+AGG | 0.491783 | 3.4:+97013874 | MS.gene064674:CDS |
| TGGACAGCCCGGCAAATATG+AGG | 0.505675 | 3.4:+97014062 | MS.gene064674:intron |
| ATATTTCAGCTTCACTTGAA+AGG | 0.518303 | 3.4:+97013822 | MS.gene064674:intron |
| GGGTTCTCTTATGGCAGGAT+GGG | 0.526091 | 3.4:+97013693 | MS.gene064674:CDS |
| ATATGAGGATATCGTGCCCT+TGG | 0.529619 | 3.4:+97014077 | MS.gene064674:CDS |
| GCAGTATCACGTAGCCAACT+TGG | 0.530756 | 3.4:+97014440 | MS.gene064674:CDS |
| ACGATATCCTCATATTTGCC+GGG | 0.546037 | 3.4:-97014069 | None:intergenic |
| TGGGTTCTCTTATGGCAGGA+TGG | 0.547179 | 3.4:+97013692 | MS.gene064674:CDS |
| TCAATGGGTTCTCTTATGGC+AGG | 0.564446 | 3.4:+97013688 | None:intergenic |
| CATGAAGAAATCATTTGGCA+TGG | 0.570197 | 3.4:+97014107 | MS.gene064674:CDS |
| CAAATGATTTCTTCATGGGG+CGG | 0.572003 | 3.4:-97014101 | None:intergenic |
| ATACTGCGATGCATACTTGT+TGG | 0.576932 | 3.4:-97014424 | None:intergenic |
| TCTGAATGAACCTCCAGTGA+TGG | 0.578917 | 3.4:+97014392 | MS.gene064674:CDS |
| GTAGGAGCTTCCATCACTGG+AGG | 0.595410 | 3.4:-97014402 | None:intergenic |
| TGCCAAATGATTTCTTCATG+GGG | 0.625447 | 3.4:-97014104 | None:intergenic |
| TCAAAATTAAACACAGGACA+TGG | 0.627591 | 3.4:+97014468 | MS.gene064674:CDS |
| TGCGATGCATACTTGTTGGT+AGG | 0.629639 | 3.4:-97014420 | None:intergenic |
| GAGATCAAAGAAGGAGACAG+AGG | 0.641594 | 3.4:+97013883 | MS.gene064674:CDS |
| CAGGTGGACAAAGAGCAACT+GGG | 0.670316 | 3.4:+97014365 | MS.gene064674:intron |
| GTATCATCAAAATTAAACAC+AGG | 0.675149 | 3.4:+97014462 | MS.gene064674:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTTATATTGAATTTAGATA+TGG | + | chr3.4:97014326-97014345 | MS.gene064674:intron | 10.0% |
| !! | AATTCATTTCGATATTTAAT+TGG | + | chr3.4:97014042-97014061 | MS.gene064674:intron | 15.0% |
| !!! | AACATTGATTTTTTAAAAGA+TGG | - | chr3.4:97014236-97014255 | None:intergenic | 15.0% |
| !! | ATAAATTGGAAATAACTTTG+AGG | - | chr3.4:97013916-97013935 | None:intergenic | 20.0% |
| !! | ATTAATTAAGTACCTGATTT+AGG | - | chr3.4:97013736-97013755 | None:intergenic | 20.0% |
| !! | GACAAAAATATGAAGAAAAA+TGG | + | chr3.4:97014135-97014154 | MS.gene064674:CDS | 20.0% |
| !!! | AAGTTATTTCCAATTTATCA+AGG | + | chr3.4:97013918-97013937 | MS.gene064674:CDS | 20.0% |
| ! | AAAATATGAAGAAAAATGGC+TGG | + | chr3.4:97014139-97014158 | MS.gene064674:CDS | 25.0% |
| ! | AATTTAGATATGGTTGCATT+GGG | + | chr3.4:97014336-97014355 | MS.gene064674:intron | 25.0% |
| ! | AGTTGTTATGATTCGAATTT+CGG | + | chr3.4:97014209-97014228 | MS.gene064674:intron | 25.0% |
| ! | GTATCATCAAAATTAAACAC+AGG | + | chr3.4:97014462-97014481 | MS.gene064674:CDS | 25.0% |
| ! | TGCTAAGACTAACAATTAAT+TGG | - | chr3.4:97014180-97014199 | None:intergenic | 25.0% |
| !!! | TAATTTTGATGATACCAAGT+TGG | - | chr3.4:97014457-97014476 | None:intergenic | 25.0% |
| !!! | TTAAAAGTATTCATGACAAC+TGG | - | chr3.4:97014285-97014304 | None:intergenic | 25.0% |
| ATATTTCAGCTTCACTTGAA+AGG | + | chr3.4:97013822-97013841 | MS.gene064674:intron | 30.0% | |
| ATGAAGAAATTGATGCTTTC+TGG | + | chr3.4:97013861-97013880 | MS.gene064674:CDS | 30.0% | |
| ATGCCAAATGATTTCTTCAT+GGG | - | chr3.4:97014108-97014127 | None:intergenic | 30.0% | |
| CAATTTATCAAGGACTATTC+AGG | + | chr3.4:97013928-97013947 | MS.gene064674:CDS | 30.0% | |
| CTGAATAGTCCTTGATAAAT+TGG | - | chr3.4:97013930-97013949 | None:intergenic | 30.0% | |
| GAATTTAGATATGGTTGCAT+TGG | + | chr3.4:97014335-97014354 | MS.gene064674:intron | 30.0% | |
| TAGCACACAAATAAAACAAC+TGG | - | chr3.4:97013984-97014003 | None:intergenic | 30.0% | |
| TCAAAATTAAACACAGGACA+TGG | + | chr3.4:97014468-97014487 | MS.gene064674:CDS | 30.0% | |
| !! | GAAAGCATCAATTTCTTCAT+TGG | - | chr3.4:97013861-97013880 | None:intergenic | 30.0% |
| !!! | ATGGCTGGTAAATTTTTTCA+TGG | + | chr3.4:97014154-97014173 | MS.gene064674:intron | 30.0% |
| AAGTGTGCAAGTAGTTGAAT+TGG | - | chr3.4:97014259-97014278 | None:intergenic | 35.0% | |
| ATTCAACTACTTGCACACTT+AGG | + | chr3.4:97014259-97014278 | MS.gene064674:intron | 35.0% | |
| CACACAAATAAAACAACTGG+CGG | - | chr3.4:97013981-97014000 | None:intergenic | 35.0% | |
| CATGAAGAAATCATTTGGCA+TGG | + | chr3.4:97014107-97014126 | MS.gene064674:CDS | 35.0% | |
| CATGCCAAATGATTTCTTCA+TGG | - | chr3.4:97014109-97014128 | None:intergenic | 35.0% | |
| TGCCAAATGATTTCTTCATG+GGG | - | chr3.4:97014107-97014126 | None:intergenic | 35.0% | |
| TTGTCTTTATGCTCTGTGTT+TGG | + | chr3.4:97013779-97013798 | MS.gene064674:intron | 35.0% | |
| AAATGATTTCTTCATGGGGC+GGG | - | chr3.4:97014103-97014122 | None:intergenic | 40.0% | |
| ACGATATCCTCATATTTGCC+GGG | - | chr3.4:97014072-97014091 | None:intergenic | 40.0% | |
| ATACTGCGATGCATACTTGT+TGG | - | chr3.4:97014427-97014446 | None:intergenic | 40.0% | |
| CAAATGATTTCTTCATGGGG+CGG | - | chr3.4:97014104-97014123 | None:intergenic | 40.0% | |
| CACACAACTGATCCTAAATC+AGG | + | chr3.4:97013721-97013740 | MS.gene064674:CDS | 40.0% | |
| CACGATATCCTCATATTTGC+CGG | - | chr3.4:97014073-97014092 | None:intergenic | 40.0% | |
| CAGCTTCACTTGAAAGGAAT+CGG | + | chr3.4:97013828-97013847 | MS.gene064674:intron | 40.0% | |
| CGATATTTAATTGGACAGCC+CGG | + | chr3.4:97014051-97014070 | MS.gene064674:intron | 40.0% | |
| ! | TGCTTTCTGGAGATCAAAGA+AGG | + | chr3.4:97013874-97013893 | MS.gene064674:CDS | 40.0% |
| ATATGAGGATATCGTGCCCT+TGG | + | chr3.4:97014077-97014096 | MS.gene064674:CDS | 45.0% | |
| CGCCCCATGAAGAAATCATT+TGG | + | chr3.4:97014102-97014121 | MS.gene064674:CDS | 45.0% | |
| GAGATCAAAGAAGGAGACAG+AGG | + | chr3.4:97013883-97013902 | MS.gene064674:CDS | 45.0% | |
| TCTGAATGAACCTCCAGTGA+TGG | + | chr3.4:97014392-97014411 | MS.gene064674:CDS | 45.0% | |
| ! | TGCGATGCATACTTGTTGGT+AGG | - | chr3.4:97014423-97014442 | None:intergenic | 45.0% |
| CAGGTGGACAAAGAGCAACT+GGG | + | chr3.4:97014365-97014384 | MS.gene064674:intron | 50.0% | |
| GCAGTATCACGTAGCCAACT+TGG | + | chr3.4:97014440-97014459 | MS.gene064674:CDS | 50.0% | |
| GGGTTCTCTTATGGCAGGAT+GGG | + | chr3.4:97013693-97013712 | MS.gene064674:CDS | 50.0% | |
| TGGGTTCTCTTATGGCAGGA+TGG | + | chr3.4:97013692-97013711 | MS.gene064674:CDS | 50.0% | |
| TTGGTAGGAGCTTCCATCAC+TGG | - | chr3.4:97014408-97014427 | None:intergenic | 50.0% | |
| GCAGGTGGACAAAGAGCAAC+TGG | + | chr3.4:97014364-97014383 | MS.gene064674:intron | 55.0% | |
| GTAGGAGCTTCCATCACTGG+AGG | - | chr3.4:97014405-97014424 | None:intergenic | 55.0% | |
| TGGACAGCCCGGCAAATATG+AGG | + | chr3.4:97014062-97014081 | MS.gene064674:intron | 55.0% | |
| TGGTTGCATTGGGTGAGTGC+AGG | + | chr3.4:97014346-97014365 | MS.gene064674:intron | 55.0% | |
| TTGCATTGGGTGAGTGCAGG+TGG | + | chr3.4:97014349-97014368 | MS.gene064674:intron | 55.0% | |
| TTCTTCATGGGGCGGGCCAA+GGG | - | chr3.4:97014096-97014115 | None:intergenic | 60.0% | |
| TTTCTTCATGGGGCGGGCCA+AGG | - | chr3.4:97014097-97014116 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 97013691 | 97014503 | 97013691 | ID=MS.gene064674 |
| chr3.4 | mRNA | 97013691 | 97014503 | 97013691 | ID=MS.gene064674.t1;Parent=MS.gene064674 |
| chr3.4 | exon | 97013691 | 97013742 | 97013691 | ID=MS.gene064674.t1.exon1;Parent=MS.gene064674.t1 |
| chr3.4 | CDS | 97013691 | 97013742 | 97013691 | ID=cds.MS.gene064674.t1;Parent=MS.gene064674.t1 |
| chr3.4 | exon | 97013831 | 97013949 | 97013831 | ID=MS.gene064674.t1.exon2;Parent=MS.gene064674.t1 |
| chr3.4 | CDS | 97013831 | 97013949 | 97013831 | ID=cds.MS.gene064674.t1;Parent=MS.gene064674.t1 |
| chr3.4 | exon | 97014069 | 97014160 | 97014069 | ID=MS.gene064674.t1.exon3;Parent=MS.gene064674.t1 |
| chr3.4 | CDS | 97014069 | 97014160 | 97014069 | ID=cds.MS.gene064674.t1;Parent=MS.gene064674.t1 |
| chr3.4 | exon | 97014368 | 97014503 | 97014368 | ID=MS.gene064674.t1.exon4;Parent=MS.gene064674.t1 |
| chr3.4 | CDS | 97014368 | 97014503 | 97014368 | ID=cds.MS.gene064674.t1;Parent=MS.gene064674.t1 |
| Gene Sequence |
| Protein sequence |
