AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene066368

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene066368.t1 MTR_5g042590 97.544 1018 18 4 1 1013 1 1016 0.0 2048
MS.gene066368.t1 MTR_6g477980 42.135 890 435 25 169 1013 261 1115 0.0 634
MS.gene066368.t1 MTR_6g477980 42.135 890 435 25 169 1013 255 1109 0.0 634
MS.gene066368.t1 MTR_1g102630 40.580 897 463 26 153 1013 116 978 0.0 612
MS.gene066368.t1 MTR_1g041345 40.223 895 467 25 152 1013 120 979 0.0 606
MS.gene066368.t1 MTR_4g056430 39.548 885 467 23 159 1013 125 971 0.0 600
MS.gene066368.t1 MTR_2g059590 38.937 922 501 25 122 1013 14 903 0.0 593
MS.gene066368.t1 MTR_2g028910 39.082 893 474 18 153 1012 173 1028 0.0 589
MS.gene066368.t1 MTR_4g056470 38.505 883 478 22 159 1013 163 1008 0.0 577
MS.gene066368.t1 MTR_4g083610 39.019 856 450 19 154 978 117 931 0.0 572
MS.gene066368.t1 MTR_4g113200 38.565 892 484 25 149 1013 22 876 0.0 568
MS.gene066368.t1 MTR_2g059790 42.384 604 304 15 433 1013 12 594 1.57e-150 460
MS.gene066368.t1 MTR_3g078647 32.075 901 520 29 159 1012 67 922 1.72e-124 402
MS.gene066368.t1 MTR_3g010650 31.579 931 526 33 135 1013 5 876 1.00e-122 396
MS.gene066368.t1 MTR_5g087870 29.657 934 542 30 139 1012 69 947 4.67e-115 377
MS.gene066368.t1 MTR_5g087890 29.967 921 538 33 138 1012 32 891 7.31e-112 367
MS.gene066368.t1 MTR_6g043180 29.849 928 541 29 138 1012 32 902 7.98e-112 367
MS.gene066368.t1 MTR_3g083300 31.366 915 532 28 151 1013 39 909 2.27e-111 366
MS.gene066368.t1 MTR_5g087870 29.221 924 537 31 139 1012 69 925 6.15e-109 360
MS.gene066368.t1 MTR_2g059590 34.061 687 413 21 122 792 14 676 8.36e-107 348
MS.gene066368.t1 MTR_1g038380 34.925 335 158 10 536 867 146 423 1.29e-40 156
MS.gene066368.t1 MTR_3g101730 41.860 172 68 4 740 911 33 172 8.58e-30 117
MS.gene066368.t1 MTR_6g037320 36.879 141 43 2 699 839 1 95 4.67e-19 84.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene066368.t1 AT1G69440 70.561 873 219 7 149 1013 148 990 0.0 1278
MS.gene066368.t1 AT1G48410 41.928 892 452 25 159 1013 186 1048 0.0 645
MS.gene066368.t1 AT1G48410 41.834 894 452 26 159 1013 186 1050 0.0 640
MS.gene066368.t1 AT1G48410 41.834 894 452 26 159 1013 186 1050 0.0 640
MS.gene066368.t1 AT5G43810 39.775 890 465 26 159 1013 135 988 0.0 594
MS.gene066368.t1 AT5G43810 39.775 890 465 26 159 1013 135 988 0.0 594
MS.gene066368.t1 AT5G43810 39.775 890 465 26 159 1013 135 988 0.0 594
MS.gene066368.t1 AT5G43810 39.775 890 465 26 159 1013 135 988 0.0 594
MS.gene066368.t1 AT2G27880 38.883 877 481 18 159 1013 154 997 0.0 588
MS.gene066368.t1 AT1G31280 36.686 845 474 19 153 976 163 967 2.21e-175 539
MS.gene066368.t1 AT1G31290 38.435 869 462 24 152 991 260 1084 7.16e-173 535
MS.gene066368.t1 AT1G31290 38.435 869 462 24 152 991 298 1122 1.70e-172 535
MS.gene066368.t1 AT1G31290 38.435 869 462 24 152 991 334 1158 4.25e-172 535
MS.gene066368.t1 AT5G21150 32.159 908 518 29 154 1013 39 896 8.69e-134 426
MS.gene066368.t1 AT2G27040 30.172 928 547 26 139 1013 45 924 2.26e-121 394
MS.gene066368.t1 AT2G27040 30.172 928 547 26 139 1013 45 924 2.26e-121 394
MS.gene066368.t1 AT5G21030 29.621 898 515 26 159 1013 31 854 3.73e-104 345
MS.gene066368.t1 AT2G32940 31.084 904 516 29 159 1013 33 878 1.91e-103 344
MS.gene066368.t1 AT5G21030 29.510 898 512 27 159 1013 31 850 8.85e-102 339

Find 193 sgRNAs with CRISPR-Local

Find 272 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCCCATCAACTTCTTTAA+AGG 0.105814 5.1:-32006490 None:intergenic
AGAACTCAAGCTTATCATTT+TGG 0.173580 5.1:-32006794 None:intergenic
ACAGCTTCTTATTTGTATAA+TGG 0.263270 5.1:+32008499 MS.gene066368:CDS
AGGAAGCAACTGAAAATCTT+TGG 0.274302 5.1:+32007372 MS.gene066368:CDS
ACAACTACAAAAGTAATAAA+AGG 0.290192 5.1:-32009070 None:intergenic
GTTCACATCTTTGAGTTAAC+TGG 0.291747 5.1:-32008349 None:intergenic
TTTCTATCTATGTAGTCATT+GGG 0.308144 5.1:+32009231 MS.gene066368:CDS
GTTAACTGGTTTATGAATCT+TGG 0.310605 5.1:-32008335 None:intergenic
ATTTCTATCTATGTAGTCAT+TGG 0.316939 5.1:+32009230 MS.gene066368:CDS
GCAGGAGGAACTAATGAAAT+TGG 0.320784 5.1:-32009376 None:intergenic
TAGAGAAATCAAGCATAAAT+TGG 0.332646 5.1:+32006692 MS.gene066368:CDS
GTTTGCTTCTGAATTTCCTT+TGG 0.334202 5.1:-32006459 None:intergenic
TTCTCTAGCAACATCCTTAG+AGG 0.337003 5.1:-32006676 None:intergenic
AGATTCTTTCCATCTCTATC+AGG 0.345858 5.1:-32007398 None:intergenic
AATATCTTTGAAACTTGTTT+TGG 0.352913 5.1:-32005578 None:intergenic
CCATCACCAAGCTTTAGTTT+TGG 0.355041 5.1:-32008173 None:intergenic
ACAAATATATTTCAAGAATA+AGG 0.359133 5.1:+32008840 MS.gene066368:CDS
CTAGATGGTCATGTCTTTGA+AGG 0.360899 5.1:+32008248 MS.gene066368:CDS
GTCGATTTGCTAGTTGGAAT+TGG 0.361272 5.1:-32006828 None:intergenic
GGCGCTTTGCCGGCATATGA+TGG 0.365628 5.1:+32006741 MS.gene066368:CDS
AGGTGGGCACTAATAAGTTT+TGG 0.365715 5.1:+32008284 MS.gene066368:CDS
TGCTCTCAATGTGGATTTCT+CGG 0.371590 5.1:+32007151 MS.gene066368:CDS
GTTCAGAGATACCGTGTCTA+TGG 0.373143 5.1:+32007341 MS.gene066368:CDS
CGGAAGAAAATGATTCGGTT+TGG 0.377730 5.1:-32008953 None:intergenic
TTAACTGGTTTATGAATCTT+GGG 0.379326 5.1:-32008334 None:intergenic
GCATGTTCATCTAGGTTTCA+TGG 0.379673 5.1:+32009040 MS.gene066368:CDS
ATCTCTGAACAGTTTCTCTA+TGG 0.379706 5.1:-32007328 None:intergenic
CTTGAGAGATCAGAGTCCTT+AGG 0.384414 5.1:+32009439 MS.gene066368:CDS
GCAGGATTATCTACATGCTT+TGG 0.385806 5.1:+32006977 MS.gene066368:CDS
GAACTTAATTTGATGAGATT+CGG 0.394282 5.1:-32008602 None:intergenic
AATTACTCCTCATCCCTCTA+AGG 0.396696 5.1:+32006662 MS.gene066368:CDS
TGCATGGAAGTTTGTGCAAA+TGG 0.396886 5.1:-32005674 None:intergenic
CCATCTGTCGCTGCTGTTGT+TGG 0.397087 5.1:+32008794 MS.gene066368:CDS
AAACTAGTCTCGAAAATTGA+TGG 0.400305 5.1:+32006900 MS.gene066368:CDS
GAAGGTAAAGGAAGAAGAGC+AGG 0.402774 5.1:-32005603 None:intergenic
TAAAACAAGCATGTTCATCT+AGG 0.403433 5.1:+32009032 MS.gene066368:CDS
TGTAGAAACCAAGTTGAGTT+TGG 0.404992 5.1:-32005548 None:intergenic
ACTCAAAGATGTGAACAATT+AGG 0.405895 5.1:+32008356 MS.gene066368:CDS
GTCATTCTATTCGAGTTCAA+TGG 0.405912 5.1:+32007049 MS.gene066368:CDS
GGAAGAAAATGATTCGGTTT+GGG 0.408175 5.1:-32008952 None:intergenic
TCGATTTGCTAGTTGGAATT+GGG 0.409961 5.1:-32006827 None:intergenic
TCTCATGACGCCTTCGATGA+TGG 0.412144 5.1:-32007630 None:intergenic
AGATAGAAATCAAATTCCTT+TGG 0.415078 5.1:-32009217 None:intergenic
CCATCCTTTAAAGAAGTTGA+TGG 0.415148 5.1:+32006486 MS.gene066368:CDS
TCATTCTATTCGAGTTCAAT+GGG 0.415940 5.1:+32007050 MS.gene066368:CDS
CAACAATACCAAACTCAACT+TGG 0.417364 5.1:+32005540 MS.gene066368:CDS
GATCGTGACGTGAAGGAGTC+AGG 0.420035 5.1:-32008211 None:intergenic
ATAACTATGACATTCAATTC+AGG 0.422709 5.1:+32007450 MS.gene066368:CDS
TTGTGACATTGTTAAGAACT+TGG 0.423163 5.1:-32008427 None:intergenic
GGTGATGTCGATTTGCTAGT+TGG 0.435407 5.1:-32006834 None:intergenic
ACTGTTGTTGTTGCTGTTGA+GGG 0.440124 5.1:-32005497 None:intergenic
TTAGGTAGAGGTGGTGTCTT+CGG 0.441067 5.1:-32009502 None:intergenic
CTGTTGTTGGTAGCATGAAC+TGG 0.441679 5.1:+32008807 MS.gene066368:CDS
TCGATACGTTTCTGCAAATA+CGG 0.441705 5.1:-32007206 None:intergenic
TGACGAGCCGGTGATGTTCA+TGG 0.443980 5.1:+32008730 MS.gene066368:CDS
CGCCAGTGGAACTTTCTAGA+TGG 0.446312 5.1:+32008233 MS.gene066368:CDS
TACTGTTGTTGTTGCTGTTG+AGG 0.448198 5.1:-32005498 None:intergenic
AAGAAGCTGTAGATTGTTTG+AGG 0.453692 5.1:-32008486 None:intergenic
AACAGTGCATCCACCAACTT+TGG 0.455056 5.1:-32008660 None:intergenic
CCTTCTTGTCCACCACAATC+TGG 0.455235 5.1:-32006561 None:intergenic
GAGTTGTCTGCCTTTGAGAA+AGG 0.457326 5.1:-32007241 None:intergenic
TGTGAAGGACAGAAGTTCCT+TGG 0.458780 5.1:+32007539 MS.gene066368:CDS
GCACATTTAGCTGCATATAG+AGG 0.463633 5.1:+32009406 MS.gene066368:CDS
GAAAGATTGTATTCTGATGA+AGG 0.465705 5.1:-32005711 None:intergenic
CTTCTACTAATTTGCAAGCA+TGG 0.466350 5.1:-32007479 None:intergenic
GAAATATATTTGTTTGCTGT+TGG 0.469845 5.1:-32008830 None:intergenic
TCTCAACTTTCACTTGATCC+TGG 0.472145 5.1:+32005732 MS.gene066368:CDS
CTTTGGTTTCCTGATAGAGA+TGG 0.476622 5.1:+32007389 MS.gene066368:CDS
CAGATCACACAAAGCTCCAT+AGG 0.479814 5.1:-32007518 None:intergenic
GAAGATTGATCGGTGTCAGC+AGG 0.480032 5.1:-32009121 None:intergenic
TCCAACCATTGCACCGAGAT+CGG 0.481016 5.1:-32008891 None:intergenic
AACTCATTACCATGTCTTGT+GGG 0.482917 5.1:+32009276 MS.gene066368:CDS
TACCCTCCAAAACTAAAGCT+TGG 0.483606 5.1:+32008167 MS.gene066368:CDS
CCATCAACTTCTTTAAAGGA+TGG 0.485273 5.1:-32006486 None:intergenic
AGAGAAATGACAAAGTTGAC+TGG 0.491533 5.1:+32008134 MS.gene066368:CDS
CTTGAATCGTCGAGAGGATG+AGG 0.492529 5.1:-32008770 None:intergenic
CAAGATGGGCTGTCAAAGAC+CGG 0.493342 5.1:+32007595 MS.gene066368:CDS
GGAGAAGTATTGAGTTTGCA+TGG 0.493972 5.1:-32005690 None:intergenic
CTCAGCTAACTTGTGAAGAA+AGG 0.494139 5.1:+32007261 MS.gene066368:CDS
GCATGGAAGTTTGTGCAAAT+GGG 0.494284 5.1:-32005673 None:intergenic
GTCTCGAAAATTGATGGGAA+GGG 0.495207 5.1:+32006906 MS.gene066368:CDS
TGTAGATAATCCTGCGGAAG+CGG 0.495429 5.1:-32006969 None:intergenic
AGTTGTTGAAGTGAAGGTAA+AGG 0.498217 5.1:-32005615 None:intergenic
GAATAACTCCTATACTCTCA+TGG 0.500379 5.1:-32007184 None:intergenic
CAATATGAAAATATTCCACC+AGG 0.501529 5.1:+32009166 MS.gene066368:CDS
GAATAGAATGACCTTCCAAC+AGG 0.501556 5.1:-32007038 None:intergenic
ACAGAATCAACCACGGTCCC+TGG 0.501941 5.1:-32009184 None:intergenic
CGAAGGGATTCCGCTTCCGC+AGG 0.505337 5.1:+32006959 MS.gene066368:CDS
CTCAAGATCAATGCCAAAGT+TGG 0.507862 5.1:+32008647 MS.gene066368:CDS
GTGAAGGACAGAAGTTCCTT+GGG 0.508035 5.1:+32007540 MS.gene066368:CDS
ACTAACTACTTGAGCAAAGA+AGG 0.508188 5.1:+32006933 MS.gene066368:CDS
ACTGCTTTCCATGAGAGTAT+AGG 0.509877 5.1:+32007176 MS.gene066368:CDS
TTGTCATTTCTCTTGAGACT+TGG 0.511045 5.1:-32008124 None:intergenic
TAAACCTTGTTATCTCCCTA+TGG 0.511898 5.1:+32007502 MS.gene066368:CDS
CATCCTTTAAAGAAGTTGAT+GGG 0.514420 5.1:+32006487 MS.gene066368:CDS
TGATAATGATTTAGCACCAA+AGG 0.516884 5.1:+32006443 MS.gene066368:intron
AAGATCAATGCCAAAGTTGG+TGG 0.519312 5.1:+32008650 MS.gene066368:CDS
AGAGCAAGTCCTTGTTGTGT+TGG 0.520077 5.1:-32007134 None:intergenic
GCCGATCTCGGTGCAATGGT+TGG 0.524340 5.1:+32008890 MS.gene066368:CDS
ATGTAGTCATTGGGGTGTGA+AGG 0.526206 5.1:+32009240 MS.gene066368:CDS
GCGCTTTGCCGGCATATGAT+GGG 0.527480 5.1:+32006742 MS.gene066368:CDS
GATATCGGTGGAGGAGCTGT+TGG 0.528041 5.1:+32007083 MS.gene066368:CDS
CTTGCAACAATTACTTCATG+TGG 0.531075 5.1:-32006534 None:intergenic
TGAGGGAAAGATGGAATCAA+AGG 0.534413 5.1:-32005639 None:intergenic
GACGAGCCGGTGATGTTCAT+GGG 0.534686 5.1:+32008731 MS.gene066368:CDS
CAAGAAATTATTGCCGATCT+CGG 0.535339 5.1:+32008878 MS.gene066368:CDS
ATGGGGAGAAGCAAAGATAT+CGG 0.538077 5.1:+32007068 MS.gene066368:CDS
GAGGAAATGTTGGTCCTACC+AGG 0.540340 5.1:+32007651 MS.gene066368:CDS
AAAGGTAGTTGTTGAAGTGA+AGG 0.542969 5.1:-32005621 None:intergenic
GCTAAATGTGCATAATATGC+AGG 0.543695 5.1:-32009394 None:intergenic
GGAGGAGCTGTTGGATTGAG+AGG 0.544646 5.1:+32007092 MS.gene066368:CDS
GATTGTTTGAGGCAATACTC+TGG 0.544839 5.1:-32008475 None:intergenic
TGTTGTTCACGTTTCTCACA+AGG 0.545398 5.1:-32006855 None:intergenic
GCTAACTTGTGAAGAAAGGA+AGG 0.550241 5.1:+32007265 MS.gene066368:CDS
TTGAAGGAACTACAATTGAA+AGG 0.550534 5.1:+32008264 MS.gene066368:CDS
GAACGAAAAGCCATCATCGA+AGG 0.552388 5.1:+32007620 MS.gene066368:CDS
AACTAGTCTCGAAAATTGAT+GGG 0.552549 5.1:+32006901 MS.gene066368:CDS
CAACTCATTACCATGTCTTG+TGG 0.554295 5.1:+32009275 MS.gene066368:CDS
ACTGAGAAATGTATACCTGT+TGG 0.554582 5.1:+32007023 MS.gene066368:CDS
ATGACATTCAATTCAGGAAG+TGG 0.554927 5.1:+32007456 MS.gene066368:CDS
CGTCTCGGAAGAAAATGATT+CGG 0.555994 5.1:-32008958 None:intergenic
CTAAGGACTCTGATCTCTCA+AGG 0.557408 5.1:-32009438 None:intergenic
TATTGCCGATCTCGGTGCAA+TGG 0.560805 5.1:+32008886 MS.gene066368:CDS
AGTCTCGAAAATTGATGGGA+AGG 0.564310 5.1:+32006905 MS.gene066368:CDS
CACAAGACATGGTAATGAGT+TGG 0.565045 5.1:-32009274 None:intergenic
CCAAAACTAAAGCTTGGTGA+TGG 0.566399 5.1:+32008173 MS.gene066368:CDS
TCACTAAGTTTAGGTAGAGG+TGG 0.567563 5.1:-32009511 None:intergenic
TGTAGTCATTGGGGTGTGAA+GGG 0.568049 5.1:+32009241 MS.gene066368:CDS
TTCTGTGATTACTCATCCAA+AGG 0.568582 5.1:+32009201 MS.gene066368:CDS
CACTGGCGATCGTGACGTGA+AGG 0.568663 5.1:-32008218 None:intergenic
CCAGATTGTGGTGGACAAGA+AGG 0.569720 5.1:+32006561 MS.gene066368:CDS
TCTCTAGCAACATCCTTAGA+GGG 0.573670 5.1:-32006675 None:intergenic
TTCTATCTATGTAGTCATTG+GGG 0.573801 5.1:+32009232 MS.gene066368:CDS
GAGAAATGACAAAGTTGACT+GGG 0.575964 5.1:+32008135 MS.gene066368:CDS
TCTCGAAAATTGATGGGAAG+GGG 0.576888 5.1:+32006907 MS.gene066368:CDS
CCAACAACAGCAGCGACAGA+TGG 0.580449 5.1:-32008794 None:intergenic
AAGGTAAAGGAAGAAGAGCA+GGG 0.582117 5.1:-32005602 None:intergenic
AGCACTTCTGAACAAACCTA+AGG 0.582641 5.1:-32009455 None:intergenic
ATTTGCTAGTTGGAATTGGG+AGG 0.582837 5.1:-32006824 None:intergenic
GCAACATCCTTAGAGGGATG+AGG 0.583299 5.1:-32006669 None:intergenic
AAGGAACTACAATTGAAAGG+TGG 0.584900 5.1:+32008267 MS.gene066368:CDS
GATTCGGATACAAGCAGCAC+TGG 0.585921 5.1:-32008586 None:intergenic
CAAAGCGCCCGATAAAATCT+CGG 0.585982 5.1:-32006727 None:intergenic
AGGAACTACAATTGAAAGGT+GGG 0.592411 5.1:+32008268 MS.gene066368:CDS
AAACTAAAGCTTGGTGATGG+AGG 0.594557 5.1:+32008176 MS.gene066368:CDS
GGCGTCATGAGAGGAAATGT+TGG 0.595199 5.1:+32007641 MS.gene066368:CDS
AGAAGCAAAGATATCGGTGG+AGG 0.598026 5.1:+32007074 MS.gene066368:CDS
AGATCACACAAAGCTCCATA+GGG 0.598624 5.1:-32007517 None:intergenic
ATCATCGAAGGCGTCATGAG+AGG 0.605292 5.1:+32007632 MS.gene066368:CDS
TTCTTAGAGACCTTTCTCAA+AGG 0.606629 5.1:+32007231 MS.gene066368:CDS
CACATGAAGTAATTGTTGCA+AGG 0.609332 5.1:+32006535 MS.gene066368:CDS
ATAGAGATGGAAAGAATCTG+AGG 0.612806 5.1:+32007402 MS.gene066368:CDS
GACCATCTAGAAAGTTCCAC+TGG 0.615247 5.1:-32008235 None:intergenic
TACAACACCGACACTTGTCT+CGG 0.616242 5.1:-32008561 None:intergenic
TCAGCTCCCATGAACATCAC+CGG 0.616771 5.1:-32008737 None:intergenic
TGAGCAAAGAAGGTGACGAA+GGG 0.621730 5.1:+32006943 MS.gene066368:CDS
GAAACTGTCGGATGATCAGA+CGG 0.621779 5.1:+32007562 MS.gene066368:CDS
TTGAGCAAAGAAGGTGACGA+AGG 0.622404 5.1:+32006942 MS.gene066368:CDS
AAAAGACGCGGTAATTGAGA+AGG 0.623652 5.1:-32008701 None:intergenic
CTTCACGTCACGATCGCCAG+TGG 0.626226 5.1:+32008219 MS.gene066368:CDS
AAGATGGGCTGTCAAAGACC+GGG 0.633687 5.1:+32007596 MS.gene066368:CDS
GAGTTTCGCTAACACCGTCT+CGG 0.634375 5.1:-32008973 None:intergenic
GAAGTTCCTTGGGAAACTGT+CGG 0.634410 5.1:+32007550 MS.gene066368:CDS
CGAATTGCCGAGACAAGTGT+CGG 0.636497 5.1:+32008554 MS.gene066368:CDS
ACTTGGATTGATTCAAACTG+CGG 0.638604 5.1:-32008410 None:intergenic
ACAGGTATACATTTCTCAGT+CGG 0.639287 5.1:-32007020 None:intergenic
GAGCTTTGTGTGATCTGTGA+AGG 0.639487 5.1:+32007524 MS.gene066368:CDS
AGAAATGTATACCTGTTGGA+AGG 0.640017 5.1:+32007027 MS.gene066368:CDS
GAATCAACCACGGTCCCTGG+TGG 0.640697 5.1:-32009181 None:intergenic
AGCTCCATAGGGAGATAACA+AGG 0.643271 5.1:-32007506 None:intergenic
ACAAGGACTTGCTCTCAATG+TGG 0.644006 5.1:+32007142 MS.gene066368:CDS
AGTCTTAGACCAACACAACA+AGG 0.644925 5.1:+32007125 MS.gene066368:CDS
AAATATTCCACCAGGGACCG+TGG 0.646415 5.1:+32009174 MS.gene066368:CDS
GTTGTGCGTCGAAGATTGAT+CGG 0.651284 5.1:-32009131 None:intergenic
GCTTTGCAGTTATACCTGGT+AGG 0.654107 5.1:-32007665 None:intergenic
GGAAGAAGCTTATTTCAACA+AGG 0.658895 5.1:+32006508 MS.gene066368:CDS
CTTTGCCGGCATATGATGGG+AGG 0.659001 5.1:+32006745 MS.gene066368:CDS
AAATGTGCATAATATGCAGG+AGG 0.662875 5.1:-32009391 None:intergenic
GGGAGAAGCAAAGATATCGG+TGG 0.666198 5.1:+32007071 MS.gene066368:CDS
AATATGAAAATATTCCACCA+GGG 0.673069 5.1:+32009167 MS.gene066368:CDS
GATCATCCGACAGTTTCCCA+AGG 0.673171 5.1:-32007556 None:intergenic
TTGGCAAGGAGAGAGATCAC+AGG 0.686931 5.1:-32006585 None:intergenic
GTTGCAAGGAGACCAGATTG+TGG 0.688799 5.1:+32006549 MS.gene066368:CDS
GATGGACTTGAATCGTCGAG+AGG 0.693236 5.1:-32008776 None:intergenic
GGGGTGTGAAGGGAACAAGT+AGG 0.696897 5.1:+32009251 MS.gene066368:CDS
CATTCTATTCGAGTTCAATG+GGG 0.697995 5.1:+32007051 MS.gene066368:CDS
TCGTCAATGTTGAAAAGACG+CGG 0.703990 5.1:-32008713 None:intergenic
GAATAAGGTCTCAAACACAC+AGG 0.710521 5.1:+32008855 MS.gene066368:CDS
CCTCAGTTAAGCCATAGACA+CGG 0.718867 5.1:-32007352 None:intergenic
CCGTGTCTATGGCTTAACTG+AGG 0.719089 5.1:+32007352 MS.gene066368:CDS
GCAAGGAGACCAGATTGTGG+TGG 0.720550 5.1:+32006552 MS.gene066368:CDS
AAAGCATGTAGATAATCCTG+CGG 0.726216 5.1:-32006975 None:intergenic
AGTAATCACAGAATCAACCA+CGG 0.768855 5.1:-32009191 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATCATTATCATAAATAAGA+TGG - chr5.1:32006434-32006453 None:intergenic 15.0%
!! ACAAATATATTTCAAGAATA+AGG + chr5.1:32008840-32008859 MS.gene066368:CDS 15.0%
!! CATCTATATTAAAAAAAACA+AGG - chr5.1:32005853-32005872 None:intergenic 15.0%
!!! TTAAATTTACTTTGACTTAT+TGG + chr5.1:32005924-32005943 MS.gene066368:intron 15.0%
!! ACAACTACAAAAGTAATAAA+AGG - chr5.1:32009073-32009092 None:intergenic 20.0%
!!! AATATCTTTGAAACTTGTTT+TGG - chr5.1:32005581-32005600 None:intergenic 20.0%
!!! ACTTTTTAAACATTGCATTT+AGG + chr5.1:32005995-32006014 MS.gene066368:intron 20.0%
!!! ATTTGTGTTTTACTTTTGTT+AGG + chr5.1:32009344-32009363 MS.gene066368:CDS 20.0%
!!! CTTTTTAAACATTGCATTTA+GGG + chr5.1:32005996-32006015 MS.gene066368:intron 20.0%
!!! TCACTGTTCTTTTTTTTTTT+TGG + chr5.1:32005772-32005791 MS.gene066368:intron 20.0%
!!! TTATCATTTTGGAATTCAAT+TGG - chr5.1:32006786-32006805 None:intergenic 20.0%
! AAAAAAAGAACAGTGAAGAA+TGG - chr5.1:32005768-32005787 None:intergenic 25.0%
! AATATGAAAATATTCCACCA+GGG + chr5.1:32009167-32009186 MS.gene066368:CDS 25.0%
! AATCAAACTTCACCAAAAAA+TGG - chr5.1:32006611-32006630 None:intergenic 25.0%
! AGATAGAAATCAAATTCCTT+TGG - chr5.1:32009220-32009239 None:intergenic 25.0%
! ATAACTATGACATTCAATTC+AGG + chr5.1:32007450-32007469 MS.gene066368:CDS 25.0%
! ATTTCTATCTATGTAGTCAT+TGG + chr5.1:32009230-32009249 MS.gene066368:CDS 25.0%
! CAAAATCTCACAAACAAAAA+TGG + chr5.1:32005435-32005454 MS.gene066368:CDS 25.0%
! CTTAAAAAACCAAACAAACA+AGG - chr5.1:32005896-32005915 None:intergenic 25.0%
! CTTATGTGAAGAATGTTATT+AGG - chr5.1:32006337-32006356 None:intergenic 25.0%
! TAATGGAGAAAAAACACAAA+GGG + chr5.1:32008516-32008535 MS.gene066368:CDS 25.0%
! TAGAGAAATCAAGCATAAAT+TGG + chr5.1:32006692-32006711 MS.gene066368:CDS 25.0%
! TCCATGTAATCACTTAATAA+CGG - chr5.1:32007921-32007940 None:intergenic 25.0%
! TGAAAATGTAAGTTCTTGTT+GGG + chr5.1:32006090-32006109 MS.gene066368:intron 25.0%
! TTTCTATCTATGTAGTCATT+GGG + chr5.1:32009231-32009250 MS.gene066368:CDS 25.0%
!! ACAGCTTCTTATTTGTATAA+TGG + chr5.1:32008499-32008518 MS.gene066368:CDS 25.0%
!! ATAATGGAGAAAAAACACAA+AGG + chr5.1:32008515-32008534 MS.gene066368:CDS 25.0%
!! CAAATTAAGTTCACAGTTTT+TGG + chr5.1:32008613-32008632 MS.gene066368:CDS 25.0%
!! GAACTTAATTTGATGAGATT+CGG - chr5.1:32008605-32008624 None:intergenic 25.0%
!! GTGAACAACATAAACTTTTT+CGG + chr5.1:32006868-32006887 MS.gene066368:CDS 25.0%
!! TGAAGATTTTTATCAAGAAG+TGG + chr5.1:32008922-32008941 MS.gene066368:CDS 25.0%
!! TTAACTGGTTTATGAATCTT+GGG - chr5.1:32008337-32008356 None:intergenic 25.0%
!! TTGATGTTTTCACTAAGTTT+AGG - chr5.1:32009523-32009542 None:intergenic 25.0%
!!! ATTTTGGTTAGTGTTTTTGT+TGG - chr5.1:32005421-32005440 None:intergenic 25.0%
!!! CATTTTTGTTTGTGAGATTT+TGG - chr5.1:32005437-32005456 None:intergenic 25.0%
!!! GAAATATATTTGTTTGCTGT+TGG - chr5.1:32008833-32008852 None:intergenic 25.0%
!!! TGTTCCAGTTTATTTTACAA+GGG - chr5.1:32007858-32007877 None:intergenic 25.0%
AAAATGTAAGTTCTTGTTGG+GGG + chr5.1:32006092-32006111 MS.gene066368:intron 30.0%
AAACTTCACCAAAAAATGGT+TGG - chr5.1:32006607-32006626 None:intergenic 30.0%
AAGTGAAATGATCACTTGTT+TGG + chr5.1:32006217-32006236 MS.gene066368:intron 30.0%
ACAGTATTTAAGTGATTACG+TGG + chr5.1:32007966-32007985 MS.gene066368:intron 30.0%
ACCGTTATTAAGTGATTACA+TGG + chr5.1:32007917-32007936 MS.gene066368:intron 30.0%
ACTCAAAGATGTGAACAATT+AGG + chr5.1:32008356-32008375 MS.gene066368:CDS 30.0%
ATCACTTAATAACGGTTTCA+TGG - chr5.1:32007913-32007932 None:intergenic 30.0%
ATGAAAAGAACAGTGAAGAA+TGG - chr5.1:32005804-32005823 None:intergenic 30.0%
CAATATGAAAATATTCCACC+AGG + chr5.1:32009166-32009185 MS.gene066368:CDS 30.0%
GAAAATGTAAGTTCTTGTTG+GGG + chr5.1:32006091-32006110 MS.gene066368:intron 30.0%
GAAAGATTGTATTCTGATGA+AGG - chr5.1:32005714-32005733 None:intergenic 30.0%
GTGAAAATGTAAGTTCTTGT+TGG + chr5.1:32006089-32006108 MS.gene066368:intron 30.0%
TAAAACAAGCATGTTCATCT+AGG + chr5.1:32009032-32009051 MS.gene066368:CDS 30.0%
TATTTAAGTGATTACGTGGA+CGG + chr5.1:32007970-32007989 MS.gene066368:intron 30.0%
TGATTACATGGACAGTTAAA+TGG + chr5.1:32007929-32007948 MS.gene066368:intron 30.0%
TTCTATCTATGTAGTCATTG+GGG + chr5.1:32009232-32009251 MS.gene066368:CDS 30.0%
TTGAAGGAACTACAATTGAA+AGG + chr5.1:32008264-32008283 MS.gene066368:CDS 30.0%
TTGTCCCTTGTAAAATAAAC+TGG + chr5.1:32007851-32007870 MS.gene066368:intron 30.0%
TTGTGACATTGTTAAGAACT+TGG - chr5.1:32008430-32008449 None:intergenic 30.0%
TTTACAAGGGACAAAAAGTT+GGG - chr5.1:32007845-32007864 None:intergenic 30.0%
! AAAAGTAATCCTTTTGACAG+TGG + chr5.1:32008077-32008096 MS.gene066368:intron 30.0%
! AAACTAGTCTCGAAAATTGA+TGG + chr5.1:32006900-32006919 MS.gene066368:CDS 30.0%
! AACTAGTCTCGAAAATTGAT+GGG + chr5.1:32006901-32006920 MS.gene066368:CDS 30.0%
! AGAACTCAAGCTTATCATTT+TGG - chr5.1:32006797-32006816 None:intergenic 30.0%
! CATCCTTTAAAGAAGTTGAT+GGG + chr5.1:32006487-32006506 MS.gene066368:CDS 30.0%
! CTTTTGTAGTTGTGCAAAAA+AGG + chr5.1:32009080-32009099 MS.gene066368:CDS 30.0%
! GTTAACTGGTTTATGAATCT+TGG - chr5.1:32008338-32008357 None:intergenic 30.0%
! TAACAATGCCGAGATTTTAT+CGG + chr5.1:32006719-32006738 MS.gene066368:CDS 30.0%
! TCATTCTATTCGAGTTCAAT+GGG + chr5.1:32007050-32007069 MS.gene066368:CDS 30.0%
! TGATAATGATTTAGCACCAA+AGG + chr5.1:32006443-32006462 MS.gene066368:intron 30.0%
! TTTTACAAGGGACAAAAAGT+TGG - chr5.1:32007846-32007865 None:intergenic 30.0%
! TTTTCACTAAGTTTAGGTAG+AGG - chr5.1:32009517-32009536 None:intergenic 30.0%
!! TTCTTTAAGCCTTGTTTGTT+TGG + chr5.1:32005884-32005903 MS.gene066368:intron 30.0%
!!! AAATGGGTTTTGAGATTTTG+AGG - chr5.1:32005660-32005679 None:intergenic 30.0%
!!! AATGGGTTTTGAGATTTTGA+GGG - chr5.1:32005659-32005678 None:intergenic 30.0%
!!! GTGTTCCAGTTTATTTTACA+AGG - chr5.1:32007859-32007878 None:intergenic 30.0%
!!! TTCACAGTTTTTGGCTAATT+TGG + chr5.1:32008622-32008641 MS.gene066368:CDS 30.0%
AAACAAGGAATGCAAGCAAA+AGG - chr5.1:32005838-32005857 None:intergenic 35.0%
AAAGCATGTAGATAATCCTG+CGG - chr5.1:32006978-32006997 None:intergenic 35.0%
AAAGGTAGTTGTTGAAGTGA+AGG - chr5.1:32005624-32005643 None:intergenic 35.0%
AAATGTAAGTTCTTGTTGGG+GGG + chr5.1:32006093-32006112 MS.gene066368:intron 35.0%
AACAAGGAATGCAAGCAAAA+GGG - chr5.1:32005837-32005856 None:intergenic 35.0%
AACTCATTACCATGTCTTGT+GGG + chr5.1:32009276-32009295 MS.gene066368:CDS 35.0%
AAGAAGCTGTAGATTGTTTG+AGG - chr5.1:32008489-32008508 None:intergenic 35.0%
AAGGAACTACAATTGAAAGG+TGG + chr5.1:32008267-32008286 MS.gene066368:CDS 35.0%
AATAACGGTTTCATGGTTTG+TGG - chr5.1:32007906-32007925 None:intergenic 35.0%
ACAGGTATACATTTCTCAGT+CGG - chr5.1:32007023-32007042 None:intergenic 35.0%
ACGGCAAAAATACTCAAGAT+GGG + chr5.1:32007581-32007600 MS.gene066368:CDS 35.0%
ACTAACTACTTGAGCAAAGA+AGG + chr5.1:32006933-32006952 MS.gene066368:CDS 35.0%
ACTGAGAAATGTATACCTGT+TGG + chr5.1:32007023-32007042 MS.gene066368:CDS 35.0%
AGAAATGTATACCTGTTGGA+AGG + chr5.1:32007027-32007046 MS.gene066368:CDS 35.0%
AGAGAAATGACAAAGTTGAC+TGG + chr5.1:32008134-32008153 MS.gene066368:CDS 35.0%
AGATTCTTTCCATCTCTATC+AGG - chr5.1:32007401-32007420 None:intergenic 35.0%
AGGAACTACAATTGAAAGGT+GGG + chr5.1:32008268-32008287 MS.gene066368:CDS 35.0%
AGGAAGCAACTGAAAATCTT+TGG + chr5.1:32007372-32007391 MS.gene066368:CDS 35.0%
AGTAATCACAGAATCAACCA+CGG - chr5.1:32009194-32009213 None:intergenic 35.0%
AGTTGTTGAAGTGAAGGTAA+AGG - chr5.1:32005618-32005637 None:intergenic 35.0%
ATAGAGATGGAAAGAATCTG+AGG + chr5.1:32007402-32007421 MS.gene066368:CDS 35.0%
ATCTCTGAACAGTTTCTCTA+TGG - chr5.1:32007331-32007350 None:intergenic 35.0%
ATGACATTCAATTCAGGAAG+TGG + chr5.1:32007456-32007475 MS.gene066368:CDS 35.0%
CAACAATACCAAACTCAACT+TGG + chr5.1:32005540-32005559 MS.gene066368:CDS 35.0%
CAAGAAATTATTGCCGATCT+CGG + chr5.1:32008878-32008897 MS.gene066368:CDS 35.0%
CACATGAAGTAATTGTTGCA+AGG + chr5.1:32006535-32006554 MS.gene066368:CDS 35.0%
CTTCCCATCAACTTCTTTAA+AGG - chr5.1:32006493-32006512 None:intergenic 35.0%
CTTGCAACAATTACTTCATG+TGG - chr5.1:32006537-32006556 None:intergenic 35.0%
GAATAACTCCTATACTCTCA+TGG - chr5.1:32007187-32007206 None:intergenic 35.0%
GAGAAATGACAAAGTTGACT+GGG + chr5.1:32008135-32008154 MS.gene066368:CDS 35.0%
GGAAGAAGCTTATTTCAACA+AGG + chr5.1:32006508-32006527 MS.gene066368:CDS 35.0%
GTTTGCTTCTGAATTTCCTT+TGG - chr5.1:32006462-32006481 None:intergenic 35.0%
TCGATACGTTTCTGCAAATA+CGG - chr5.1:32007209-32007228 None:intergenic 35.0%
TTCTGTGATTACTCATCCAA+AGG + chr5.1:32009201-32009220 MS.gene066368:CDS 35.0%
TTCTTAGAGACCTTTCTCAA+AGG + chr5.1:32007231-32007250 MS.gene066368:CDS 35.0%
! AACAATGCCGAGATTTTATC+GGG + chr5.1:32006720-32006739 MS.gene066368:CDS 35.0%
! ACTTGGATTGATTCAAACTG+CGG - chr5.1:32008413-32008432 None:intergenic 35.0%
! CATTCTATTCGAGTTCAATG+GGG + chr5.1:32007051-32007070 MS.gene066368:CDS 35.0%
! CCATCAACTTCTTTAAAGGA+TGG - chr5.1:32006489-32006508 None:intergenic 35.0%
! CCATCCTTTAAAGAAGTTGA+TGG + chr5.1:32006486-32006505 MS.gene066368:CDS 35.0%
! CTTCTACTAATTTGCAAGCA+TGG - chr5.1:32007482-32007501 None:intergenic 35.0%
! GGAGTTTGAATTCATTTTCC+TGG - chr5.1:32008106-32008125 None:intergenic 35.0%
! GTCATTCTATTCGAGTTCAA+TGG + chr5.1:32007049-32007068 MS.gene066368:CDS 35.0%
! GTTCACATCTTTGAGTTAAC+TGG - chr5.1:32008352-32008371 None:intergenic 35.0%
! GTTTTACAAAGTTCTGCAAG+AGG + chr5.1:32008997-32009016 MS.gene066368:CDS 35.0%
! TAAACCTTGTTATCTCCCTA+TGG + chr5.1:32007502-32007521 MS.gene066368:CDS 35.0%
! TATTTTCATCCCACAAGACA+TGG - chr5.1:32009288-32009307 None:intergenic 35.0%
! TGTAGAAACCAAGTTGAGTT+TGG - chr5.1:32005551-32005570 None:intergenic 35.0%
! TTGTCATTTCTCTTGAGACT+TGG - chr5.1:32008127-32008146 None:intergenic 35.0%
!! ATGCTTTGGATGTAGTTTTG+AGG + chr5.1:32006991-32007010 MS.gene066368:CDS 35.0%
!! GGAAGAAAATGATTCGGTTT+GGG - chr5.1:32008955-32008974 None:intergenic 35.0%
!! TCGATTTGCTAGTTGGAATT+GGG - chr5.1:32006830-32006849 None:intergenic 35.0%
!! TTGAGATTTTGAGGGAAAGA+TGG - chr5.1:32005651-32005670 None:intergenic 35.0%
!!! TGCTTTGGATGTAGTTTTGA+GGG + chr5.1:32006992-32007011 MS.gene066368:CDS 35.0%
AAAAGACGCGGTAATTGAGA+AGG - chr5.1:32008704-32008723 None:intergenic 40.0%
AAGAATGGTGCTTACTTACC+AGG - chr5.1:32005753-32005772 None:intergenic 40.0%
AAGATCAATGCCAAAGTTGG+TGG + chr5.1:32008650-32008669 MS.gene066368:CDS 40.0%
AAGGTAAAGGAAGAAGAGCA+GGG - chr5.1:32005605-32005624 None:intergenic 40.0%
AATTACTCCTCATCCCTCTA+AGG + chr5.1:32006662-32006681 MS.gene066368:CDS 40.0%
AGATCACACAAAGCTCCATA+GGG - chr5.1:32007520-32007539 None:intergenic 40.0%
AGCACTTCTGAACAAACCTA+AGG - chr5.1:32009458-32009477 None:intergenic 40.0%
AGTCTCGAAAATTGATGGGA+AGG + chr5.1:32006905-32006924 MS.gene066368:CDS 40.0%
AGTCTTAGACCAACACAACA+AGG + chr5.1:32007125-32007144 MS.gene066368:CDS 40.0%
ATGGGGAGAAGCAAAGATAT+CGG + chr5.1:32007068-32007087 MS.gene066368:CDS 40.0%
CAACTCATTACCATGTCTTG+TGG + chr5.1:32009275-32009294 MS.gene066368:CDS 40.0%
CGTCTCGGAAGAAAATGATT+CGG - chr5.1:32008961-32008980 None:intergenic 40.0%
CTAGATGGTCATGTCTTTGA+AGG + chr5.1:32008248-32008267 MS.gene066368:CDS 40.0%
CTCAAGATCAATGCCAAAGT+TGG + chr5.1:32008647-32008666 MS.gene066368:CDS 40.0%
GAATAAGGTCTCAAACACAC+AGG + chr5.1:32008855-32008874 MS.gene066368:CDS 40.0%
GAATAGAATGACCTTCCAAC+AGG - chr5.1:32007041-32007060 None:intergenic 40.0%
GACGGCAAAAATACTCAAGA+TGG + chr5.1:32007580-32007599 MS.gene066368:CDS 40.0%
GCACATTTAGCTGCATATAG+AGG + chr5.1:32009406-32009425 MS.gene066368:CDS 40.0%
GCAGGATTATCTACATGCTT+TGG + chr5.1:32006977-32006996 MS.gene066368:CDS 40.0%
GCATGGAAGTTTGTGCAAAT+GGG - chr5.1:32005676-32005695 None:intergenic 40.0%
GCATGTTCATCTAGGTTTCA+TGG + chr5.1:32009040-32009059 MS.gene066368:CDS 40.0%
GTCTCGAAAATTGATGGGAA+GGG + chr5.1:32006906-32006925 MS.gene066368:CDS 40.0%
TACCCTCCAAAACTAAAGCT+TGG + chr5.1:32008167-32008186 MS.gene066368:CDS 40.0%
TAGTGCTTTGCAGTTATACC+TGG - chr5.1:32007672-32007691 None:intergenic 40.0%
TCACCAAAAAATGGTTGGCA+AGG - chr5.1:32006602-32006621 None:intergenic 40.0%
TCACTAAGTTTAGGTAGAGG+TGG - chr5.1:32009514-32009533 None:intergenic 40.0%
TCGTCAATGTTGAAAAGACG+CGG - chr5.1:32008716-32008735 None:intergenic 40.0%
TCTCAACTTTCACTTGATCC+TGG + chr5.1:32005732-32005751 MS.gene066368:CDS 40.0%
TCTCGAAAATTGATGGGAAG+GGG + chr5.1:32006907-32006926 MS.gene066368:CDS 40.0%
TCTCTAGCAACATCCTTAGA+GGG - chr5.1:32006678-32006697 None:intergenic 40.0%
TGAGGGAAAGATGGAATCAA+AGG - chr5.1:32005642-32005661 None:intergenic 40.0%
TGATTACGTGGACGGTTAAA+TGG + chr5.1:32007978-32007997 MS.gene066368:intron 40.0%
TGCATGGAAGTTTGTGCAAA+TGG - chr5.1:32005677-32005696 None:intergenic 40.0%
TGCTCTCAATGTGGATTTCT+CGG + chr5.1:32007151-32007170 MS.gene066368:CDS 40.0%
TGTTGTTCACGTTTCTCACA+AGG - chr5.1:32006858-32006877 None:intergenic 40.0%
TTCTCTAGCAACATCCTTAG+AGG - chr5.1:32006679-32006698 None:intergenic 40.0%
TTGTTGGGGGGAATACTTTA+AGG + chr5.1:32006105-32006124 MS.gene066368:intron 40.0%
! ACTGCTTTCCATGAGAGTAT+AGG + chr5.1:32007176-32007195 MS.gene066368:CDS 40.0%
! ACTGTTGTTGTTGCTGTTGA+GGG - chr5.1:32005500-32005519 None:intergenic 40.0%
! AGGTGGGCACTAATAAGTTT+TGG + chr5.1:32008284-32008303 MS.gene066368:CDS 40.0%
! ATTTGCTAGTTGGAATTGGG+AGG - chr5.1:32006827-32006846 None:intergenic 40.0%
! CACAAGACATGGTAATGAGT+TGG - chr5.1:32009277-32009296 None:intergenic 40.0%
! CCAAAACTAAAGCTTGGTGA+TGG + chr5.1:32008173-32008192 MS.gene066368:CDS 40.0%
! CGAATCATTTTCTTCCGAGA+CGG + chr5.1:32008959-32008978 MS.gene066368:CDS 40.0%
! CTCAGCTAACTTGTGAAGAA+AGG + chr5.1:32007261-32007280 MS.gene066368:CDS 40.0%
! CTTTGGTTTCCTGATAGAGA+TGG + chr5.1:32007389-32007408 MS.gene066368:CDS 40.0%
! GATTGTTTGAGGCAATACTC+TGG - chr5.1:32008478-32008497 None:intergenic 40.0%
! GCTAACTTGTGAAGAAAGGA+AGG + chr5.1:32007265-32007284 MS.gene066368:CDS 40.0%
! TACTGTTGTTGTTGCTGTTG+AGG - chr5.1:32005501-32005520 None:intergenic 40.0%
! TCTTTTCAACATTGACGAGC+CGG + chr5.1:32008718-32008737 MS.gene066368:CDS 40.0%
! TTTTTCCTCCCATCATATGC+CGG - chr5.1:32006753-32006772 None:intergenic 40.0%
!! AAACTAAAGCTTGGTGATGG+AGG + chr5.1:32008176-32008195 MS.gene066368:CDS 40.0%
!! CCATCACCAAGCTTTAGTTT+TGG - chr5.1:32008176-32008195 None:intergenic 40.0%
!! CGGAAGAAAATGATTCGGTT+TGG - chr5.1:32008956-32008975 None:intergenic 40.0%
!! GGAGAAGTATTGAGTTTGCA+TGG - chr5.1:32005693-32005712 None:intergenic 40.0%
!! GTCGATTTGCTAGTTGGAAT+TGG - chr5.1:32006831-32006850 None:intergenic 40.0%
!! TGCAAAAAAGGCATCACACA+AGG + chr5.1:32009092-32009111 MS.gene066368:CDS 40.0%
!! TGGGCACTAATAAGTTTTGG+AGG + chr5.1:32008287-32008306 MS.gene066368:CDS 40.0%
!!! CACCAAGCTTTAGTTTTGGA+GGG - chr5.1:32008172-32008191 None:intergenic 40.0%
!!! TCACCAAGCTTTAGTTTTGG+AGG - chr5.1:32008173-32008192 None:intergenic 40.0%
!!! TCTCCTTGCCAACCATTTTT+TGG + chr5.1:32006596-32006615 MS.gene066368:CDS 40.0%
AACAGTGCATCCACCAACTT+TGG - chr5.1:32008663-32008682 None:intergenic 45.0%
AGAAGCAAAGATATCGGTGG+AGG + chr5.1:32007074-32007093 MS.gene066368:CDS 45.0%
AGCTCCATAGGGAGATAACA+AGG - chr5.1:32007509-32007528 None:intergenic 45.0%
ATGTAGTCATTGGGGTGTGA+AGG + chr5.1:32009240-32009259 MS.gene066368:CDS 45.0%
CAGATCACACAAAGCTCCAT+AGG - chr5.1:32007521-32007540 None:intergenic 45.0%
CCTCAGTTAAGCCATAGACA+CGG - chr5.1:32007355-32007374 None:intergenic 45.0%
CTAAGGACTCTGATCTCTCA+AGG - chr5.1:32009441-32009460 None:intergenic 45.0%
CTTGAGAGATCAGAGTCCTT+AGG + chr5.1:32009439-32009458 MS.gene066368:CDS 45.0%
GAAACTGTCGGATGATCAGA+CGG + chr5.1:32007562-32007581 MS.gene066368:CDS 45.0%
GAACGAAAAGCCATCATCGA+AGG + chr5.1:32007620-32007639 MS.gene066368:CDS 45.0%
GAAGGTAAAGGAAGAAGAGC+AGG - chr5.1:32005606-32005625 None:intergenic 45.0%
GAAGTTCCTTGGGAAACTGT+CGG + chr5.1:32007550-32007569 MS.gene066368:CDS 45.0%
GACCATCTAGAAAGTTCCAC+TGG - chr5.1:32008238-32008257 None:intergenic 45.0%
GCTTTGCAGTTATACCTGGT+AGG - chr5.1:32007668-32007687 None:intergenic 45.0%
GTGAAGGACAGAAGTTCCTT+GGG + chr5.1:32007540-32007559 MS.gene066368:CDS 45.0%
GTTCAGAGATACCGTGTCTA+TGG + chr5.1:32007341-32007360 MS.gene066368:CDS 45.0%
GTTGTGCGTCGAAGATTGAT+CGG - chr5.1:32009134-32009153 None:intergenic 45.0%
TACAACACCGACACTTGTCT+CGG - chr5.1:32008564-32008583 None:intergenic 45.0%
TGAGCAAAGAAGGTGACGAA+GGG + chr5.1:32006943-32006962 MS.gene066368:CDS 45.0%
TGTAGATAATCCTGCGGAAG+CGG - chr5.1:32006972-32006991 None:intergenic 45.0%
TGTAGTCATTGGGGTGTGAA+GGG + chr5.1:32009241-32009260 MS.gene066368:CDS 45.0%
TGTGAAGGACAGAAGTTCCT+TGG + chr5.1:32007539-32007558 MS.gene066368:CDS 45.0%
TTAGGTAGAGGTGGTGTCTT+CGG - chr5.1:32009505-32009524 None:intergenic 45.0%
TTGAGCAAAGAAGGTGACGA+AGG + chr5.1:32006942-32006961 MS.gene066368:CDS 45.0%
! ACAAGGACTTGCTCTCAATG+TGG + chr5.1:32007142-32007161 MS.gene066368:CDS 45.0%
! ACGGTTTCATGGTTTGTGGA+TGG - chr5.1:32007902-32007921 None:intergenic 45.0%
! AGAGCAAGTCCTTGTTGTGT+TGG - chr5.1:32007137-32007156 None:intergenic 45.0%
! ATGATGGCTTTTCGTTCTCC+CGG - chr5.1:32007617-32007636 None:intergenic 45.0%
! CAAAGCGCCCGATAAAATCT+CGG - chr5.1:32006730-32006749 None:intergenic 45.0%
! CATGGTTTGTGGATGGATGT+TGG - chr5.1:32007895-32007914 None:intergenic 45.0%
! GAGCTTTGTGTGATCTGTGA+AGG + chr5.1:32007524-32007543 MS.gene066368:CDS 45.0%
! GAGTTGTCTGCCTTTGAGAA+AGG - chr5.1:32007244-32007263 None:intergenic 45.0%
! GGGATATGAGACTTTTGCTC+AGG - chr5.1:32008317-32008336 None:intergenic 45.0%
!! CTGTTGTTGGTAGCATGAAC+TGG + chr5.1:32008807-32008826 MS.gene066368:CDS 45.0%
!! GGACAGTTAAATGGACTACG+TGG + chr5.1:32007938-32007957 MS.gene066368:intron 45.0%
!! GGTGATGTCGATTTGCTAGT+TGG - chr5.1:32006837-32006856 None:intergenic 45.0%
AAATATTCCACCAGGGACCG+TGG + chr5.1:32009174-32009193 MS.gene066368:CDS 50.0%
CCGTGTCTATGGCTTAACTG+AGG + chr5.1:32007352-32007371 MS.gene066368:CDS 50.0%
CCTTCTTGTCCACCACAATC+TGG - chr5.1:32006564-32006583 None:intergenic 50.0%
CGAATTGCCGAGACAAGTGT+CGG + chr5.1:32008554-32008573 MS.gene066368:CDS 50.0%
CGCCAGTGGAACTTTCTAGA+TGG + chr5.1:32008233-32008252 MS.gene066368:CDS 50.0%
CTTGAATCGTCGAGAGGATG+AGG - chr5.1:32008773-32008792 None:intergenic 50.0%
GAGGAAATGTTGGTCCTACC+AGG + chr5.1:32007651-32007670 MS.gene066368:CDS 50.0%
GAGTTTCGCTAACACCGTCT+CGG - chr5.1:32008976-32008995 None:intergenic 50.0%
GATCATCCGACAGTTTCCCA+AGG - chr5.1:32007559-32007578 None:intergenic 50.0%
GATTCGGATACAAGCAGCAC+TGG - chr5.1:32008589-32008608 None:intergenic 50.0%
GCAACATCCTTAGAGGGATG+AGG - chr5.1:32006672-32006691 None:intergenic 50.0%
GGCGTCATGAGAGGAAATGT+TGG + chr5.1:32007641-32007660 MS.gene066368:CDS 50.0%
GGGAGAAGCAAAGATATCGG+TGG + chr5.1:32007071-32007090 MS.gene066368:CDS 50.0%
GTTGCAAGGAGACCAGATTG+TGG + chr5.1:32006549-32006568 MS.gene066368:CDS 50.0%
TCAGCTCCCATGAACATCAC+CGG - chr5.1:32008740-32008759 None:intergenic 50.0%
TCCAACCATTGCACCGAGAT+CGG - chr5.1:32008894-32008913 None:intergenic 50.0%
TCCTGGTCACCACTGTCAAA+AGG - chr5.1:32008089-32008108 None:intergenic 50.0%
TCTCATGACGCCTTCGATGA+TGG - chr5.1:32007633-32007652 None:intergenic 50.0%
TTGGCAAGGAGAGAGATCAC+AGG - chr5.1:32006588-32006607 None:intergenic 50.0%
! GATGGACTTGAATCGTCGAG+AGG - chr5.1:32008779-32008798 None:intergenic 50.0%
! GATTTTATCGGGCGCTTTGC+CGG + chr5.1:32006731-32006750 MS.gene066368:CDS 50.0%
! TATTGCCGATCTCGGTGCAA+TGG + chr5.1:32008886-32008905 MS.gene066368:CDS 50.0%
! TCCTTTTGACAGTGGTGACC+AGG + chr5.1:32008085-32008104 MS.gene066368:intron 50.0%
!! AAGATGGGCTGTCAAAGACC+GGG + chr5.1:32007596-32007615 MS.gene066368:CDS 50.0%
!! ATCATCGAAGGCGTCATGAG+AGG + chr5.1:32007632-32007651 MS.gene066368:CDS 50.0%
!! CAAGATGGGCTGTCAAAGAC+CGG + chr5.1:32007595-32007614 MS.gene066368:CDS 50.0%
!! CCAGATTGTGGTGGACAAGA+AGG + chr5.1:32006561-32006580 MS.gene066368:CDS 50.0%
!! GAAGATTGATCGGTGTCAGC+AGG - chr5.1:32009124-32009143 None:intergenic 50.0%
!! GGACGGTTAAATGGACTACG+TGG + chr5.1:32007987-32008006 MS.gene066368:intron 50.0%
ACAGAATCAACCACGGTCCC+TGG - chr5.1:32009187-32009206 None:intergenic 55.0%
CCAACAACAGCAGCGACAGA+TGG - chr5.1:32008797-32008816 None:intergenic 55.0%
CCATCTGTCGCTGCTGTTGT+TGG + chr5.1:32008794-32008813 MS.gene066368:CDS 55.0%
GACGAGCCGGTGATGTTCAT+GGG + chr5.1:32008731-32008750 MS.gene066368:CDS 55.0%
GATATCGGTGGAGGAGCTGT+TGG + chr5.1:32007083-32007102 MS.gene066368:CDS 55.0%
GATCGTGACGTGAAGGAGTC+AGG - chr5.1:32008214-32008233 None:intergenic 55.0%
GCAAGGAGACCAGATTGTGG+TGG + chr5.1:32006552-32006571 MS.gene066368:CDS 55.0%
GCGCTTTGCCGGCATATGAT+GGG + chr5.1:32006742-32006761 MS.gene066368:CDS 55.0%
GGAGGAGCTGTTGGATTGAG+AGG + chr5.1:32007092-32007111 MS.gene066368:CDS 55.0%
GGGGTGTGAAGGGAACAAGT+AGG + chr5.1:32009251-32009270 MS.gene066368:CDS 55.0%
TGACGAGCCGGTGATGTTCA+TGG + chr5.1:32008730-32008749 MS.gene066368:CDS 55.0%
! CTTTGCCGGCATATGATGGG+AGG + chr5.1:32006745-32006764 MS.gene066368:CDS 55.0%
CACTGGCGATCGTGACGTGA+AGG - chr5.1:32008221-32008240 None:intergenic 60.0%
CTTCACGTCACGATCGCCAG+TGG + chr5.1:32008219-32008238 MS.gene066368:CDS 60.0%
GAATCAACCACGGTCCCTGG+TGG - chr5.1:32009184-32009203 None:intergenic 60.0%
GGCGCTTTGCCGGCATATGA+TGG + chr5.1:32006741-32006760 MS.gene066368:CDS 60.0%
! GCCGATCTCGGTGCAATGGT+TGG + chr5.1:32008890-32008909 MS.gene066368:CDS 60.0%
CGAAGGGATTCCGCTTCCGC+AGG + chr5.1:32006959-32006978 MS.gene066368:CDS 65.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 32005396 32009564 32005396 ID=MS.gene066368
chr5.1 mRNA 32005396 32009564 32005396 ID=MS.gene066368.t1;Parent=MS.gene066368
chr5.1 exon 32005396 32005753 32005396 ID=MS.gene066368.t1.exon1;Parent=MS.gene066368.t1
chr5.1 CDS 32005396 32005753 32005396 ID=cds.MS.gene066368.t1;Parent=MS.gene066368.t1
chr5.1 exon 32006457 32007672 32006457 ID=MS.gene066368.t1.exon2;Parent=MS.gene066368.t1
chr5.1 CDS 32006457 32007672 32006457 ID=cds.MS.gene066368.t1;Parent=MS.gene066368.t1
chr5.1 exon 32008097 32009564 32008097 ID=MS.gene066368.t1.exon3;Parent=MS.gene066368.t1
chr5.1 CDS 32008097 32009564 32008097 ID=cds.MS.gene066368.t1;Parent=MS.gene066368.t1
Gene Sequence

>MS.gene066368

ATGGAACAACAAGAAAACTCAACCAACAAAAACACTAACCAAAATCTCACAAACAAAAATGGAACAAACTCTCATCAACATCATCATCATTACTATCATCACCCTCAACAGCAACAACAACAGTATCAGTATCAGAATCATCTTCAACAATACCAAACTCAACTTGGTTTCTACAACAACTACCAAAACAAGTTTCAAAGATATTACCCTGCTCTTCTTCCTTTACCTTCACTTCAACAACTACCTTTGATTCCATCTTTCCCTCAAAATCTCAAAACCCATTTGCACAAACTTCCATGCAAACTCAATACTTCTCCTTCATCAGAATACAATCTTTCTCAACTTTCACTTGATCCTGGTAAGTAAGCACCATTCTTCACTGTTCTTTTTTTTTTTTGGTTAAGTCCATTCTTCACTGTTCTTTTCATTTCAAACTTACCCTTTTGCTTGCATTCCTTGTTTTTTTTAATATAGATGCTAAAAATTTGTTCTTTAAGCCTTGTTTGTTTGGTTTTTTAAGAAAAAATTTTAAATTTACTTTGACTTATTGGAGAGTGTGAAAAAAATAAATTTTTTATTGTTTCTTACTCTTTTTATATACTTTTTAAACATTGCATTTAGGGTTGTAATAATTTACGATTTGTTTATATATTGTGCATTTTTTTTTTTAACATTTGTTTTGCTGTTTTTAATGTGAAAATGTAAGTTCTTGTTGGGGGGAATACTTTAAGGTCACTGTTTCACTGTATTACATTTTTTTGTAACTTGAGTGTTATGTTTTTGCTTAGTGATGTTTTTTTGTTAAAAGTAGATTTAATTTAAAGTGAAATGATCACTTGTTTGGTCTCACATCAAGTCAAAATTGATTTATTCTCAAATTTGCTTTAATAAGAAGCAACAATAATTTACTTTTTGCTTTAAGCACGTTTAGAAATTATCCTAATAACATTCTTCACATAAGATAAATCTAACTGTGACATGAAAATGATACTTAGTTATTTGAGCTTAACAATTAATTTATGCAAAAATGATGAACCATCTTATTTATGATAATGATTTAGCACCAAAGGAAATTCAGAAGCAAACTAGACCATCCTTTAAAGAAGTTGATGGGAAGAAGCTTATTTCAACAAGGAAGCCACATGAAGTAATTGTTGCAAGGAGACCAGATTGTGGTGGACAAGAAGGTCCTGTGATCTCTCTCCTTGCCAACCATTTTTTGGTGAAGTTTGATTCATCACAAAAAATATATCATTACAATGTTGAAATTACTCCTCATCCCTCTAAGGATGTTGCTAGAGAAATCAAGCATAAATTGGTAAATAACAATGCCGAGATTTTATCGGGCGCTTTGCCGGCATATGATGGGAGGAAAAATCTTTATAGTCCAATTGAATTCCAAAATGATAAGCTTGAGTTCTACATAAGCCTCCCAATTCCAACTAGCAAATCGACATCACCTTGTGAGAAACGTGAACAACATAAACTTTTTCGGATAAATATCAAACTAGTCTCGAAAATTGATGGGAAGGGGTTGACTAACTACTTGAGCAAAGAAGGTGACGAAGGGATTCCGCTTCCGCAGGATTATCTACATGCTTTGGATGTAGTTTTGAGGGAAAGTCCGACTGAGAAATGTATACCTGTTGGAAGGTCATTCTATTCGAGTTCAATGGGGAGAAGCAAAGATATCGGTGGAGGAGCTGTTGGATTGAGAGGCTTCTTTCAGAGTCTTAGACCAACACAACAAGGACTTGCTCTCAATGTGGATTTCTCGGTAACTGCTTTCCATGAGAGTATAGGAGTTATTCCGTATTTGCAGAAACGTATCGAGTTTCTTAGAGACCTTTCTCAAAGGCAGACAACTCAGCTAACTTGTGAAGAAAGGAAGGAAGTAGAGAAAACGTTGAAGAATATCAGAGTCTTTGTTTGCCATAGAGAAACTGTTCAGAGATACCGTGTCTATGGCTTAACTGAGGAAGCAACTGAAAATCTTTGGTTTCCTGATAGAGATGGAAAGAATCTGAGGCTTATGAGTTACTTTAAAGATCACTATAACTATGACATTCAATTCAGGAAGTGGCCATGCTTGCAAATTAGTAGAAGTAAACCTTGTTATCTCCCTATGGAGCTTTGTGTGATCTGTGAAGGACAGAAGTTCCTTGGGAAACTGTCGGATGATCAGACGGCAAAAATACTCAAGATGGGCTGTCAAAGACCGGGAGAACGAAAAGCCATCATCGAAGGCGTCATGAGAGGAAATGTTGGTCCTACCAGGTATAACTGCAAAGCACTACTTTCATATTTCTTGTTTATAGAATATTGTGTATGATTCTTTATACTTTCTTAGTACTTCTATTCTATGATCATTTATGTATTTGACTGAAATTTCATTCTAATTCTGTACAAAACGCACAAAGTTCAGATTAGCTCTTCAGAAGAAAAGCCCAACTTTTTGTCCCTTGTAAAATAAACTGGAACACTTGACTATCTTGACCAACATCCATCCACAAACCATGAAACCGTTATTAAGTGATTACATGGACAGTTAAATGGACTACGTGGCGTTAACAGTATTTAAGTGATTACGTGGACGGTTAAATGGACTACGTGGCGTTGACAGTGTTTAAGTGATTATTACATTGTTTTTGAATTAGTTTCTTTCTACTTTCTAATGCTTTAAAAGTAATCCTTTTGACAGTGGTGACCAGGAAAATGAATTCAAACTCCAAGTCTCAAGAGAAATGACAAAGTTGACTGGGAGAATTCTTTACCCTCCAAAACTAAAGCTTGGTGATGGAGGTCATGTGAGAAACCTGACTCCTTCACGTCACGATCGCCAGTGGAACTTTCTAGATGGTCATGTCTTTGAAGGAACTACAATTGAAAGGTGGGCACTAATAAGTTTTGGAGGCACACCTGAGCAAAAGTCTCATATCCCAAGATTCATAAACCAGTTAACTCAAAGATGTGAACAATTAGGCATTTTTCTCAACAAGAACACAATTATTAGTCCGCAGTTTGAATCAATCCAAGTTCTTAACAATGTCACAATTTTAGAATCTAAGCTCAAAAGAATCCAGAGTATTGCCTCAAACAATCTACAGCTTCTTATTTGTATAATGGAGAAAAAACACAAAGGGTATGCAGATTTGAAACGAATTGCCGAGACAAGTGTCGGTGTTGTAAGCCAGTGCTGCTTGTATCCGAATCTCATCAAATTAAGTTCACAGTTTTTGGCTAATTTGGCTCTCAAGATCAATGCCAAAGTTGGTGGATGCACTGTTGCTTTGTACAACTCGCTTCCTTCTCAATTACCGCGTCTTTTCAACATTGACGAGCCGGTGATGTTCATGGGAGCTGACGTCACGCATCCTCATCCTCTCGACGATTCAAGTCCATCTGTCGCTGCTGTTGTTGGTAGCATGAACTGGCCAACAGCAAACAAATATATTTCAAGAATAAGGTCTCAAACACACAGGCAAGAAATTATTGCCGATCTCGGTGCAATGGTTGGAGAATTGCTTGAAGATTTTTATCAAGAAGTGGAAAAACTCCCAAACCGAATCATTTTCTTCCGAGACGGTGTTAGCGAAACTCAGTTTTACAAAGTTCTGCAAGAGGAACTACAATCAATAAAACAAGCATGTTCATCTAGGTTTCATGGTTATAAACCTTTTATTACTTTTGTAGTTGTGCAAAAAAGGCATCACACAAGGTTGTTTCCTGCTGACACCGATCAATCTTCGACGCACAACAATTTTCACTTTCAATATGAAAATATTCCACCAGGGACCGTGGTTGATTCTGTGATTACTCATCCAAAGGAATTTGATTTCTATCTATGTAGTCATTGGGGTGTGAAGGGAACAAGTAGGCCAACTCATTACCATGTCTTGTGGGATGAAAATAAGTTTACTTCAGATGAACTGCAAAAGCTTGTTTACAATTTGTGTTTTACTTTTGTTAGGTGTACTAAGCCAATTTCATTAGTTCCTCCTGCATATTATGCACATTTAGCTGCATATAGAGGTAGACTCTACCTTGAGAGATCAGAGTCCTTAGGTTTGTTCAGAAGTGCTTCTACATTATCAAGAGCTGCTACGCCGAAGACACCACCTCTACCTAAACTTAGTGAAAACATCAAGAAGCTTATGTTTTACTGCTAG
Protein sequence

>MS.gene066368.t1

MEQQENSTNKNTNQNLTNKNGTNSHQHHHHYYHHPQQQQQQYQYQNHLQQYQTQLGFYNNYQNKFQRYYPALLPLPSLQQLPLIPSFPQNLKTHLHKLPCKLNTSPSSEYNLSQLSLDPAPKEIQKQTRPSFKEVDGKKLISTRKPHEVIVARRPDCGGQEGPVISLLANHFLVKFDSSQKIYHYNVEITPHPSKDVAREIKHKLVNNNAEILSGALPAYDGRKNLYSPIEFQNDKLEFYISLPIPTSKSTSPCEKREQHKLFRINIKLVSKIDGKGLTNYLSKEGDEGIPLPQDYLHALDVVLRESPTEKCIPVGRSFYSSSMGRSKDIGGGAVGLRGFFQSLRPTQQGLALNVDFSVTAFHESIGVIPYLQKRIEFLRDLSQRQTTQLTCEERKEVEKTLKNIRVFVCHRETVQRYRVYGLTEEATENLWFPDRDGKNLRLMSYFKDHYNYDIQFRKWPCLQISRSKPCYLPMELCVICEGQKFLGKLSDDQTAKILKMGCQRPGERKAIIEGVMRGNVGPTSGDQENEFKLQVSREMTKLTGRILYPPKLKLGDGGHVRNLTPSRHDRQWNFLDGHVFEGTTIERWALISFGGTPEQKSHIPRFINQLTQRCEQLGIFLNKNTIISPQFESIQVLNNVTILESKLKRIQSIASNNLQLLICIMEKKHKGYADLKRIAETSVGVVSQCCLYPNLIKLSSQFLANLALKINAKVGGCTVALYNSLPSQLPRLFNIDEPVMFMGADVTHPHPLDDSSPSVAAVVGSMNWPTANKYISRIRSQTHRQEIIADLGAMVGELLEDFYQEVEKLPNRIIFFRDGVSETQFYKVLQEELQSIKQACSSRFHGYKPFITFVVVQKRHHTRLFPADTDQSSTHNNFHFQYENIPPGTVVDSVITHPKEFDFYLCSHWGVKGTSRPTHYHVLWDENKFTSDELQKLVYNLCFTFVRCTKPISLVPPAYYAHLAAYRGRLYLERSESLGLFRSASTLSRAATPKTPPLPKLSENIKKLMFYC