Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067354.t1 | XP_013463904.1 | 98.4 | 377 | 6 | 0 | 1 | 377 | 1 | 377 | 3.60E-217 | 763.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067354.t1 | O81024 | 62.1 | 372 | 139 | 2 | 1 | 372 | 1 | 370 | 2.3e-135 | 483.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067354.t1 | A0A072V7D3 | 98.4 | 377 | 6 | 0 | 1 | 377 | 1 | 377 | 2.6e-217 | 763.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene063832 | MS.gene067354 | PPI |
| MS.gene069299 | MS.gene067354 | PPI |
| MS.gene067354 | MS.gene87205 | PPI |
| MS.gene88406 | MS.gene067354 | PPI |
| MS.gene069952 | MS.gene067354 | PPI |
| MS.gene016513 | MS.gene067354 | PPI |
| MS.gene067354 | MS.gene046260 | PPI |
| MS.gene039577 | MS.gene067354 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067354.t1 | MTR_2g054370 | 98.408 | 377 | 6 | 0 | 1 | 377 | 1 | 377 | 0.0 | 773 |
| MS.gene067354.t1 | MTR_6g039820 | 34.056 | 323 | 190 | 6 | 59 | 366 | 29 | 343 | 2.28e-54 | 182 |
| MS.gene067354.t1 | MTR_3g013430 | 34.062 | 320 | 196 | 5 | 56 | 366 | 140 | 453 | 2.58e-54 | 187 |
| MS.gene067354.t1 | MTR_8g041330 | 33.537 | 328 | 203 | 6 | 48 | 366 | 41 | 362 | 2.91e-54 | 184 |
| MS.gene067354.t1 | MTR_1g097850 | 32.923 | 325 | 179 | 8 | 57 | 365 | 27 | 328 | 1.50e-48 | 167 |
| MS.gene067354.t1 | MTR_6g040230 | 35.897 | 234 | 133 | 4 | 142 | 366 | 271 | 496 | 1.16e-39 | 147 |
| MS.gene067354.t1 | MTR_1g097850 | 30.864 | 243 | 134 | 6 | 57 | 285 | 27 | 249 | 1.32e-29 | 115 |
| MS.gene067354.t1 | MTR_8g041220 | 35.484 | 93 | 53 | 2 | 267 | 353 | 40 | 131 | 6.24e-11 | 60.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067354.t1 | AT2G26830 | 63.324 | 349 | 128 | 0 | 24 | 372 | 22 | 370 | 4.37e-170 | 479 |
| MS.gene067354.t1 | AT4G09760 | 34.936 | 312 | 187 | 6 | 64 | 366 | 38 | 342 | 4.43e-55 | 185 |
| MS.gene067354.t1 | AT4G09760 | 34.936 | 312 | 187 | 6 | 64 | 366 | 38 | 342 | 4.43e-55 | 185 |
| MS.gene067354.t1 | AT1G74320 | 32.209 | 326 | 198 | 9 | 56 | 368 | 32 | 347 | 2.30e-53 | 180 |
| MS.gene067354.t1 | AT1G71697 | 29.191 | 346 | 225 | 7 | 31 | 365 | 8 | 344 | 4.67e-47 | 164 |
| MS.gene067354.t1 | AT4G09760 | 29.688 | 192 | 127 | 4 | 64 | 253 | 38 | 223 | 1.90e-21 | 92.8 |
| MS.gene067354.t1 | AT4G09760 | 28.571 | 175 | 117 | 4 | 64 | 236 | 38 | 206 | 1.73e-17 | 80.9 |
Find 71 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCCAGATATCATTCCATAAT+TGG | 0.099867 | 2.3:-36220094 | None:intergenic |
| TGATATTGTTCATTCATATT+TGG | 0.103949 | 2.3:-36225704 | None:intergenic |
| TCTCACAATGACTTGCTTTC+TGG | 0.116891 | 2.3:+36224665 | MS.gene067354:CDS |
| TTGCAGTTGTTTGGCTATTT+TGG | 0.127146 | 2.3:-36220033 | None:intergenic |
| GCTAAGTGGTTTGGAATATT+TGG | 0.153201 | 2.3:+36218909 | MS.gene067354:CDS |
| GCAGCATCTGTTCTAGAATT+TGG | 0.157391 | 2.3:+36221411 | MS.gene067354:intron |
| TTTAAAGTTGAGAAAATTTC+TGG | 0.169623 | 2.3:+36217510 | MS.gene067354:CDS |
| CCCAATTATGGAATGATATC+TGG | 0.258364 | 2.3:+36220093 | MS.gene067354:CDS |
| TCTACTCATTCCTTTCTAAA+AGG | 0.267387 | 2.3:-36227079 | None:intergenic |
| CCAGATATCATTCCATAATT+GGG | 0.278940 | 2.3:-36220093 | None:intergenic |
| GATATTGTTCATTCATATTT+GGG | 0.294085 | 2.3:-36225703 | None:intergenic |
| TACATCACTGCTGCAGGATT+TGG | 0.299530 | 2.3:+36218885 | MS.gene067354:CDS |
| CAAGATAATCAAAATCTATT+GGG | 0.308596 | 2.3:-36226986 | None:intergenic |
| GCTCTAAGGAACCCCAATTA+TGG | 0.311734 | 2.3:+36220081 | MS.gene067354:CDS |
| CCAAGATAATCAAAATCTAT+TGG | 0.314209 | 2.3:-36226987 | None:intergenic |
| TCAATGATATGCTCGGTGTT+GGG | 0.351100 | 2.3:-36217824 | None:intergenic |
| TTCAAGACCTGGTCAAGTTT+AGG | 0.351629 | 2.3:+36217477 | MS.gene067354:CDS |
| ATGAACAATATCATTTCTTC+AGG | 0.376626 | 2.3:+36225714 | MS.gene067354:CDS |
| GCTTGTTCTGCGATTTCAAC+GGG | 0.403905 | 2.3:-36216835 | None:intergenic |
| TGATCGCTTTCGAGAATTGC+AGG | 0.404652 | 2.3:+36217844 | MS.gene067354:CDS |
| TGGTTTGGAATATTTGGAAA+TGG | 0.407658 | 2.3:+36218915 | MS.gene067354:CDS |
| TGTGGAAATTCCAGGCTCTA+AGG | 0.418229 | 2.3:+36220067 | MS.gene067354:CDS |
| GGATTTGGTGCTAAGTGGTT+TGG | 0.421899 | 2.3:+36218900 | MS.gene067354:CDS |
| ATTACCTCTTGAGGTCTGTC+TGG | 0.424572 | 2.3:-36225749 | None:intergenic |
| ATCAATGATATGCTCGGTGT+TGG | 0.428005 | 2.3:-36217825 | None:intergenic |
| ATTATAACCATCAGATTGTA+TGG | 0.431135 | 2.3:+36217800 | MS.gene067354:CDS |
| AAGGGAAAGAGAAGGATCAA+TGG | 0.457811 | 2.3:-36216899 | None:intergenic |
| ATCAAATACATCACTGCTGC+AGG | 0.459460 | 2.3:+36218879 | MS.gene067354:CDS |
| TGGGTGGAAAAGTTAAGATC+TGG | 0.470594 | 2.3:+36216809 | MS.gene067354:CDS |
| GCGCATACTCTTGCTCCATC+AGG | 0.478124 | 2.3:+36218960 | MS.gene067354:CDS |
| AGATATGCGAGAACCAAAAC+TGG | 0.481384 | 2.3:+36220007 | MS.gene067354:intron |
| AATTGGGGTTCCTTAGAGCC+TGG | 0.484263 | 2.3:-36220077 | None:intergenic |
| TTCGATACAACGAGTACAAA+AGG | 0.487153 | 2.3:+36227021 | MS.gene067354:CDS |
| AGCTTGTTCTGCGATTTCAA+CGG | 0.491199 | 2.3:-36216836 | None:intergenic |
| TTACAAGACAGTTTCATTCA+AGG | 0.491822 | 2.3:+36221455 | MS.gene067354:CDS |
| AACTACAGAGGCTATGACAT+AGG | 0.495161 | 2.3:+36225518 | MS.gene067354:CDS |
| TCTTAAAACTTTGTATGTTG+AGG | 0.497216 | 2.3:+36226809 | MS.gene067354:CDS |
| TGATGAAATTTATGAGCTCC+AGG | 0.497219 | 2.3:+36221485 | MS.gene067354:CDS |
| TTTCACGGTGTGGAAATTCC+AGG | 0.499815 | 2.3:+36220059 | MS.gene067354:CDS |
| TGCAGCAGTGATGTATTTGA+TGG | 0.502189 | 2.3:-36218877 | None:intergenic |
| CTGCAGGATTTGGTGCTAAG+TGG | 0.517680 | 2.3:+36218895 | MS.gene067354:CDS |
| TTGAAATCGCAGAACAAGCT+CGG | 0.518498 | 2.3:+36216839 | MS.gene067354:CDS |
| GGATCAATGGAGAGAAGAGA+AGG | 0.518927 | 2.3:-36216886 | None:intergenic |
| CAATGATATGCTCGGTGTTG+GGG | 0.521995 | 2.3:-36217823 | None:intergenic |
| ATGACGAGAGGTGTCATTTG+AGG | 0.522794 | 2.3:-36216922 | None:intergenic |
| GAAAGAAAAGCTTGTATCGA+TGG | 0.527863 | 2.3:+36227046 | MS.gene067354:CDS |
| AATCACTTTGCAGAATATGC+TGG | 0.528361 | 2.3:+36225542 | MS.gene067354:CDS |
| CTGAAGGTTTCAGTTAAACA+AGG | 0.534256 | 2.3:+36217761 | MS.gene067354:CDS |
| CAGATATCATTCCATAATTG+GGG | 0.535171 | 2.3:-36220092 | None:intergenic |
| CGAGAGGTGTCATTTGAGGA+AGG | 0.544148 | 2.3:-36216918 | None:intergenic |
| ACTACAGAGGCTATGACATA+GGG | 0.549695 | 2.3:+36225519 | MS.gene067354:CDS |
| ATAATGATTAATCACGAAGA+AGG | 0.551700 | 2.3:+36224692 | MS.gene067354:CDS |
| ACAGCCAGACAGACCTCAAG+AGG | 0.575109 | 2.3:+36225745 | MS.gene067354:CDS |
| TGAATGCAGTAATTTGTGCA+AGG | 0.578273 | 2.3:+36217449 | MS.gene067354:intron |
| TGAGGTAAATACATATGCAC+TGG | 0.582931 | 2.3:+36226827 | MS.gene067354:CDS |
| ACTGTGATTATTACCTCTTG+AGG | 0.585155 | 2.3:-36225758 | None:intergenic |
| CTCATTCCTTTCTAAAAGGT+AGG | 0.596387 | 2.3:-36227075 | None:intergenic |
| AAGATAATCAAAATCTATTG+GGG | 0.603378 | 2.3:-36226985 | None:intergenic |
| AAAGCGATCAATGATATGCT+CGG | 0.607018 | 2.3:-36217831 | None:intergenic |
| GAGAGGTGTCATTTGAGGAA+GGG | 0.608013 | 2.3:-36216917 | None:intergenic |
| ATTTGAGGAAGGGAAAGAGA+AGG | 0.609170 | 2.3:-36216907 | None:intergenic |
| AAAGTTGAGAAAATTTCTGG+AGG | 0.619890 | 2.3:+36217513 | MS.gene067354:CDS |
| GCAAGGACATGTTCAAGACC+TGG | 0.621936 | 2.3:+36217466 | MS.gene067354:CDS |
| GTTGGGGCCATACAATCTGA+TGG | 0.622325 | 2.3:-36217807 | None:intergenic |
| AGAATCACCTAAACTTGACC+AGG | 0.623881 | 2.3:-36217484 | None:intergenic |
| GAAGGTTTCAGTTAAACAAG+GGG | 0.631491 | 2.3:+36217763 | MS.gene067354:CDS |
| TCAATAATACTAATAATGGG+TGG | 0.633491 | 2.3:+36216793 | None:intergenic |
| TGAAGGTTTCAGTTAAACAA+GGG | 0.637167 | 2.3:+36217762 | MS.gene067354:CDS |
| AGAGTTACTGACATGACGAG+AGG | 0.684298 | 2.3:-36216934 | None:intergenic |
| CAGAGACCAGAAAAGGTGTG+AGG | 0.696052 | 2.3:-36226850 | None:intergenic |
| TATGCATCATACAACTACAG+AGG | 0.704451 | 2.3:+36225506 | MS.gene067354:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.3 | gene | 36216808 | 36227100 | 36216808 | ID=MS.gene067354 |
| chr2.3 | mRNA | 36216808 | 36227100 | 36216808 | ID=MS.gene067354.t1;Parent=MS.gene067354 |
| chr2.3 | exon | 36216808 | 36216944 | 36216808 | ID=MS.gene067354.t1.exon1;Parent=MS.gene067354.t1 |
| chr2.3 | CDS | 36216808 | 36216944 | 36216808 | ID=cds.MS.gene067354.t1;Parent=MS.gene067354.t1 |
| chr2.3 | exon | 36217458 | 36217549 | 36217458 | ID=MS.gene067354.t1.exon2;Parent=MS.gene067354.t1 |
| chr2.3 | CDS | 36217458 | 36217549 | 36217458 | ID=cds.MS.gene067354.t1;Parent=MS.gene067354.t1 |
| chr2.3 | exon | 36217759 | 36217865 | 36217759 | ID=MS.gene067354.t1.exon3;Parent=MS.gene067354.t1 |
| chr2.3 | CDS | 36217759 | 36217865 | 36217759 | ID=cds.MS.gene067354.t1;Parent=MS.gene067354.t1 |
| chr2.3 | exon | 36218876 | 36218981 | 36218876 | ID=MS.gene067354.t1.exon4;Parent=MS.gene067354.t1 |
| chr2.3 | CDS | 36218876 | 36218981 | 36218876 | ID=cds.MS.gene067354.t1;Parent=MS.gene067354.t1 |
| chr2.3 | exon | 36220009 | 36220131 | 36220009 | ID=MS.gene067354.t1.exon5;Parent=MS.gene067354.t1 |
| chr2.3 | CDS | 36220009 | 36220131 | 36220009 | ID=cds.MS.gene067354.t1;Parent=MS.gene067354.t1 |
| chr2.3 | exon | 36221415 | 36221506 | 36221415 | ID=MS.gene067354.t1.exon6;Parent=MS.gene067354.t1 |
| chr2.3 | CDS | 36221415 | 36221506 | 36221415 | ID=cds.MS.gene067354.t1;Parent=MS.gene067354.t1 |
| chr2.3 | exon | 36224629 | 36224713 | 36224629 | ID=MS.gene067354.t1.exon7;Parent=MS.gene067354.t1 |
| chr2.3 | CDS | 36224629 | 36224713 | 36224629 | ID=cds.MS.gene067354.t1;Parent=MS.gene067354.t1 |
| chr2.3 | exon | 36225480 | 36225585 | 36225480 | ID=MS.gene067354.t1.exon8;Parent=MS.gene067354.t1 |
| chr2.3 | CDS | 36225480 | 36225585 | 36225480 | ID=cds.MS.gene067354.t1;Parent=MS.gene067354.t1 |
| chr2.3 | exon | 36225700 | 36225766 | 36225700 | ID=MS.gene067354.t1.exon9;Parent=MS.gene067354.t1 |
| chr2.3 | CDS | 36225700 | 36225766 | 36225700 | ID=cds.MS.gene067354.t1;Parent=MS.gene067354.t1 |
| chr2.3 | exon | 36226795 | 36226887 | 36226795 | ID=MS.gene067354.t1.exon10;Parent=MS.gene067354.t1 |
| chr2.3 | CDS | 36226795 | 36226887 | 36226795 | ID=cds.MS.gene067354.t1;Parent=MS.gene067354.t1 |
| chr2.3 | exon | 36226975 | 36227100 | 36226975 | ID=MS.gene067354.t1.exon11;Parent=MS.gene067354.t1 |
| chr2.3 | CDS | 36226975 | 36227100 | 36226975 | ID=cds.MS.gene067354.t1;Parent=MS.gene067354.t1 |
| Gene Sequence |
| Protein sequence |
