Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068361.t1 | XP_013444607.1 | 98.1 | 105 | 2 | 0 | 1 | 105 | 73 | 177 | 4.50E-32 | 147.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068361.t1 | Q1ECI2 | 47.9 | 94 | 41 | 3 | 1 | 93 | 79 | 165 | 4.4e-12 | 72.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068361.t1 | A0A072TMI1 | 98.1 | 105 | 2 | 0 | 1 | 105 | 73 | 177 | 3.2e-32 | 147.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049153 | MS.gene068361 | 0.803504 | 3.38E-49 | -1.69E-46 |
| MS.gene051568 | MS.gene068361 | 0.804445 | 2.15E-49 | -1.69E-46 |
| MS.gene051860 | MS.gene068361 | 0.80658 | 7.68E-50 | -1.69E-46 |
| MS.gene052011 | MS.gene068361 | -0.803681 | 3.10E-49 | -1.69E-46 |
| MS.gene053169 | MS.gene068361 | 0.814644 | 1.39E-51 | -1.69E-46 |
| MS.gene054010 | MS.gene068361 | 0.809022 | 2.32E-50 | -1.69E-46 |
| MS.gene054183 | MS.gene068361 | 0.830913 | 2.25E-55 | -1.69E-46 |
| MS.gene054556 | MS.gene068361 | 0.847498 | 1.11E-59 | -1.69E-46 |
| MS.gene055358 | MS.gene068361 | 0.802442 | 5.60E-49 | -1.69E-46 |
| MS.gene055604 | MS.gene068361 | 0.802554 | 5.31E-49 | -1.69E-46 |
| MS.gene055666 | MS.gene068361 | 0.812248 | 4.66E-51 | -1.69E-46 |
| MS.gene056447 | MS.gene068361 | 0.805118 | 1.56E-49 | -1.69E-46 |
| MS.gene057469 | MS.gene068361 | 0.826586 | 2.50E-54 | -1.69E-46 |
| MS.gene059420 | MS.gene068361 | 0.808582 | 2.89E-50 | -1.69E-46 |
| MS.gene059609 | MS.gene068361 | 0.841555 | 4.43E-58 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068361.t1 | MTR_8g027465 | 98.095 | 105 | 2 | 0 | 1 | 105 | 73 | 177 | 2.14e-72 | 213 |
| MS.gene068361.t1 | MTR_7g117690 | 57.798 | 109 | 31 | 5 | 1 | 104 | 62 | 160 | 4.47e-21 | 82.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068361.t1 | AT1G01250 | 56.250 | 96 | 30 | 4 | 1 | 93 | 79 | 165 | 1.14e-20 | 82.0 |
Find 34 sgRNAs with CRISPR-Local
Find 35 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAGGATAGTGGCAATGATTC+TGG | 0.332754 | 8.3:+66790170 | MS.gene068361:CDS |
| TTGGGGTGAGATTGAGTTGC+CGG | 0.352671 | 8.3:+66790217 | MS.gene068361:CDS |
| TGATGAAGTGCACCGTCTTC+CGG | 0.364471 | 8.3:+66790055 | MS.gene068361:CDS |
| CGCCGTCTTTGTGTACTCCT+AGG | 0.397917 | 8.3:+66790087 | MS.gene068361:CDS |
| GGTTAAGGCTGTTGCTTCGC+CGG | 0.446564 | 8.3:+66790148 | MS.gene068361:CDS |
| TTACTCTGTTGCGGTGGTGC+CGG | 0.451618 | 8.3:+66790265 | MS.gene068361:CDS |
| GGAAGACGGTGCACTTCATC+AGG | 0.453796 | 8.3:-66790053 | None:intergenic |
| CACGTTCCATTCACAAGCTC+CGG | 0.465164 | 8.3:-66790236 | None:intergenic |
| AGCGGCTCGTATGATGGTTA+AGG | 0.472395 | 8.3:+66790133 | MS.gene068361:CDS |
| AAAGACGGCGGAGGAAGCAC+CGG | 0.484098 | 8.3:-66790074 | None:intergenic |
| GTTGCTTCGCCGGAGGATAG+TGG | 0.486958 | 8.3:+66790158 | MS.gene068361:CDS |
| GGCAATGATTCTGGTGGTAG+TGG | 0.491276 | 8.3:+66790179 | MS.gene068361:CDS |
| GGCGCCGGAGATGGCTGCAA+AGG | 0.501469 | 8.3:+66789977 | None:intergenic |
| GGCTAAAGCGGCTCGTATGA+TGG | 0.508302 | 8.3:+66790127 | MS.gene068361:CDS |
| GAGTTGCCGGAGCTTGTGAA+TGG | 0.508528 | 8.3:+66790230 | MS.gene068361:CDS |
| GTTGCTGCATATTGTTTGAA+AGG | 0.536428 | 8.3:+66790008 | MS.gene068361:CDS |
| GATTCTGGTGGTAGTGGTGG+TGG | 0.536592 | 8.3:+66790185 | MS.gene068361:CDS |
| GCCGTCTTTGTGTACTCCTA+GGG | 0.540725 | 8.3:+66790088 | MS.gene068361:CDS |
| AATGATTCTGGTGGTAGTGG+TGG | 0.546218 | 8.3:+66790182 | MS.gene068361:CDS |
| TGCTAAGGGAGTTCAATCTC+CGG | 0.574996 | 8.3:-66790284 | None:intergenic |
| GCGGAGGAAGCACCGGAAGA+CGG | 0.580891 | 8.3:-66790067 | None:intergenic |
| GAATCATTGCCACTATCCTC+CGG | 0.581285 | 8.3:-66790167 | None:intergenic |
| TAAGGCTGTTGCTTCGCCGG+AGG | 0.598672 | 8.3:+66790151 | MS.gene068361:CDS |
| GCAGCAGCTTGAATATCCCT+AGG | 0.602855 | 8.3:-66790104 | None:intergenic |
| GGAGTACACAAAGACGGCGG+AGG | 0.620739 | 8.3:-66790083 | None:intergenic |
| TAGGGATATTCAAGCTGCTG+CGG | 0.623646 | 8.3:+66790106 | MS.gene068361:CDS |
| TCAAGCTGCTGCGGCTAAAG+CGG | 0.630553 | 8.3:+66790115 | MS.gene068361:CDS |
| TCCCTAGGAGTACACAAAGA+CGG | 0.652531 | 8.3:-66790089 | None:intergenic |
| CGGAGCTTGTGAATGGAACG+TGG | 0.684103 | 8.3:+66790237 | MS.gene068361:CDS |
| GATAGTGGCAATGATTCTGG+TGG | 0.687356 | 8.3:+66790173 | MS.gene068361:CDS |
| GTGGAGTTACTCTGTTGCGG+TGG | 0.692988 | 8.3:+66790259 | MS.gene068361:CDS |
| GTGGTGGAGTTACTCTGTTG+CGG | 0.698928 | 8.3:+66790256 | MS.gene068361:CDS |
| AGCTTGTGAATGGAACGTGG+TGG | 0.768709 | 8.3:+66790240 | MS.gene068361:CDS |
| CTAGGAGTACACAAAGACGG+CGG | 0.774724 | 8.3:-66790086 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| GTTGCTGCATATTGTTTGAA+AGG | + | chr8.3:66790008-66790027 | MS.gene068361:CDS | 35.0% | |
| !!! | TGGTGGTGGTGATGATTTTT+GGG | + | chr8.3:66790199-66790218 | MS.gene068361:CDS | 40.0% |
| GAATCATTGCCACTATCCTC+CGG | - | chr8.3:66790170-66790189 | None:intergenic | 45.0% | |
| TAGGGATATTCAAGCTGCTG+CGG | + | chr8.3:66790106-66790125 | MS.gene068361:CDS | 45.0% | |
| ! | GAGGATAGTGGCAATGATTC+TGG | + | chr8.3:66790170-66790189 | MS.gene068361:CDS | 45.0% |
| ! | GATAGTGGCAATGATTCTGG+TGG | + | chr8.3:66790173-66790192 | MS.gene068361:CDS | 45.0% |
| ! | TCCCTAGGAGTACACAAAGA+CGG | - | chr8.3:66790092-66790111 | None:intergenic | 45.0% |
| !! | AATGATTCTGGTGGTAGTGG+TGG | + | chr8.3:66790182-66790201 | MS.gene068361:CDS | 45.0% |
| !! | GGTGGTGGTGATGATTTTTG+GGG | + | chr8.3:66790200-66790219 | MS.gene068361:CDS | 45.0% |
| !!! | GTGGTGGTGGTGATGATTTT+TGG | + | chr8.3:66790198-66790217 | MS.gene068361:CDS | 45.0% |
| AGCGGCTCGTATGATGGTTA+AGG | + | chr8.3:66790133-66790152 | MS.gene068361:CDS | 50.0% | |
| AGCTTGTGAATGGAACGTGG+TGG | + | chr8.3:66790240-66790259 | MS.gene068361:CDS | 50.0% | |
| CACGTTCCATTCACAAGCTC+CGG | - | chr8.3:66790239-66790258 | None:intergenic | 50.0% | |
| GCAGCAGCTTGAATATCCCT+AGG | - | chr8.3:66790107-66790126 | None:intergenic | 50.0% | |
| GCCGTCTTTGTGTACTCCTA+GGG | + | chr8.3:66790088-66790107 | MS.gene068361:CDS | 50.0% | |
| GGCAATGATTCTGGTGGTAG+TGG | + | chr8.3:66790179-66790198 | MS.gene068361:CDS | 50.0% | |
| TGATGAAGTGCACCGTCTTC+CGG | + | chr8.3:66790055-66790074 | MS.gene068361:CDS | 50.0% | |
| ! | CTAGGAGTACACAAAGACGG+CGG | - | chr8.3:66790089-66790108 | None:intergenic | 50.0% |
| ! | GTGGTGGAGTTACTCTGTTG+CGG | + | chr8.3:66790256-66790275 | MS.gene068361:CDS | 50.0% |
| ! | TTGGGGTGAGATTGAGTTGC+CGG | + | chr8.3:66790217-66790236 | MS.gene068361:CDS | 50.0% |
| CGCCGTCTTTGTGTACTCCT+AGG | + | chr8.3:66790087-66790106 | MS.gene068361:CDS | 55.0% | |
| CGGAGCTTGTGAATGGAACG+TGG | + | chr8.3:66790237-66790256 | MS.gene068361:CDS | 55.0% | |
| GAGTTGCCGGAGCTTGTGAA+TGG | + | chr8.3:66790230-66790249 | MS.gene068361:CDS | 55.0% | |
| GGAAGACGGTGCACTTCATC+AGG | - | chr8.3:66790056-66790075 | None:intergenic | 55.0% | |
| GGCTAAAGCGGCTCGTATGA+TGG | + | chr8.3:66790127-66790146 | MS.gene068361:CDS | 55.0% | |
| GTGGAGTTACTCTGTTGCGG+TGG | + | chr8.3:66790259-66790278 | MS.gene068361:CDS | 55.0% | |
| TCAAGCTGCTGCGGCTAAAG+CGG | + | chr8.3:66790115-66790134 | MS.gene068361:CDS | 55.0% | |
| TTACTCTGTTGCGGTGGTGC+CGG | + | chr8.3:66790265-66790284 | MS.gene068361:CDS | 55.0% | |
| ! | GGTTAAGGCTGTTGCTTCGC+CGG | + | chr8.3:66790148-66790167 | MS.gene068361:CDS | 55.0% |
| !! | GATTCTGGTGGTAGTGGTGG+TGG | + | chr8.3:66790185-66790204 | MS.gene068361:CDS | 55.0% |
| GGAGTACACAAAGACGGCGG+AGG | - | chr8.3:66790086-66790105 | None:intergenic | 60.0% | |
| GTTGCTTCGCCGGAGGATAG+TGG | + | chr8.3:66790158-66790177 | MS.gene068361:CDS | 60.0% | |
| ! | AAAGACGGCGGAGGAAGCAC+CGG | - | chr8.3:66790077-66790096 | None:intergenic | 60.0% |
| ! | TAAGGCTGTTGCTTCGCCGG+AGG | + | chr8.3:66790151-66790170 | MS.gene068361:CDS | 60.0% |
| !! | GCGGAGGAAGCACCGGAAGA+CGG | - | chr8.3:66790070-66790089 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 66789987 | 66790304 | 66789987 | ID=MS.gene068361 |
| chr8.3 | mRNA | 66789987 | 66790304 | 66789987 | ID=MS.gene068361.t1;Parent=MS.gene068361 |
| chr8.3 | exon | 66789987 | 66790304 | 66789987 | ID=MS.gene068361.t1.exon1;Parent=MS.gene068361.t1 |
| chr8.3 | CDS | 66789987 | 66790304 | 66789987 | ID=cds.MS.gene068361.t1;Parent=MS.gene068361.t1 |
| Gene Sequence |
| Protein sequence |
