Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene069096.t1 | XP_013453681.1 | 90.5 | 42 | 4 | 0 | 8 | 49 | 30 | 71 | 8.70E-15 | 89 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene069096.t1 | A0A072UDK7 | 90.5 | 42 | 4 | 0 | 8 | 49 | 30 | 71 | 6.3e-15 | 89.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049549 | MS.gene069096 | 0.833002 | 6.85E-56 | -1.69E-46 |
| MS.gene051127 | MS.gene069096 | 0.801648 | 8.15E-49 | -1.69E-46 |
| MS.gene052211 | MS.gene069096 | 0.845184 | 4.76E-59 | -1.69E-46 |
| MS.gene052482 | MS.gene069096 | -0.808158 | 3.55E-50 | -1.69E-46 |
| MS.gene052486 | MS.gene069096 | -0.813541 | 2.43E-51 | -1.69E-46 |
| MS.gene052606 | MS.gene069096 | 0.815976 | 7.01E-52 | -1.69E-46 |
| MS.gene053420 | MS.gene069096 | 0.812201 | 4.78E-51 | -1.69E-46 |
| MS.gene05470 | MS.gene069096 | -0.80874 | 2.67E-50 | -1.69E-46 |
| MS.gene055154 | MS.gene069096 | 0.814126 | 1.81E-51 | -1.69E-46 |
| MS.gene055158 | MS.gene069096 | 0.810909 | 9.12E-51 | -1.69E-46 |
| MS.gene055240 | MS.gene069096 | 0.814261 | 1.69E-51 | -1.69E-46 |
| MS.gene05589 | MS.gene069096 | -0.804515 | 2.08E-49 | -1.69E-46 |
| MS.gene056504 | MS.gene069096 | -0.816318 | 5.88E-52 | -1.69E-46 |
| MS.gene056748 | MS.gene069096 | 0.803783 | 2.96E-49 | -1.69E-46 |
| MS.gene057768 | MS.gene069096 | 0.811384 | 7.19E-51 | -1.69E-46 |
| MS.gene058111 | MS.gene069096 | 0.819798 | 9.61E-53 | -1.69E-46 |
| MS.gene058171 | MS.gene069096 | 0.80017 | 1.63E-48 | -1.69E-46 |
| MS.gene058175 | MS.gene069096 | 0.80282 | 4.68E-49 | -1.69E-46 |
| MS.gene059955 | MS.gene069096 | 0.813929 | 2.00E-51 | -1.69E-46 |
| MS.gene060762 | MS.gene069096 | -0.8018 | 7.59E-49 | -1.69E-46 |
| MS.gene06077 | MS.gene069096 | -0.82381 | 1.13E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 14 sgRNAs with CRISPR-Local
Find 29 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGCAATGTGTTGTGCAAGAC+TGG | 0.375088 | 5.3:-19176467 | MS.gene069096:CDS |
| TTATGCAGTGATGGTGGAGC+TGG | 0.397441 | 5.3:-19175831 | MS.gene069096:intron |
| TCACTGCTACTCTCAATAAC+TGG | 0.438274 | 5.3:+19175804 | None:intergenic |
| AGGACTGAAGGGCCTGATGC+TGG | 0.490624 | 5.3:-19176418 | MS.gene069096:CDS |
| TATGCAGTGATGGTGGAGCT+GGG | 0.557903 | 5.3:-19175830 | MS.gene069096:intron |
| ATTGAGAGTAGCAGTGACAA+TGG | 0.563224 | 5.3:-19175798 | MS.gene069096:CDS |
| GTTGGACATGTGTTATAACT+AGG | 0.581816 | 5.3:-19176438 | MS.gene069096:CDS |
| GTGTTATAACTAGGACTGAA+GGG | 0.595455 | 5.3:-19176429 | MS.gene069096:CDS |
| AAACTCTTATGCAGTGATGG+TGG | 0.602169 | 5.3:-19175837 | MS.gene069096:intron |
| TGTGTTATAACTAGGACTGA+AGG | 0.610701 | 5.3:-19176430 | MS.gene069096:CDS |
| GTGCAAGACTGGGTAAAGGT+TGG | 0.650845 | 5.3:-19176456 | MS.gene069096:CDS |
| TGTTGTGCAAGACTGGGTAA+AGG | 0.660097 | 5.3:-19176460 | MS.gene069096:CDS |
| GCAATGTGTTGTGCAAGACT+GGG | 0.665640 | 5.3:-19176466 | MS.gene069096:CDS |
| AGTGAAACATTCTGCAAGTG+TGG | 0.671634 | 5.3:-19175774 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTAATTATTTGTTTGTAATA+TGG | - | chr5.3:19176110-19176129 | MS.gene069096:intron | 10.0% |
| !! | TAATATGAAATTGAGATTTA+AGG | + | chr5.3:19176235-19176254 | None:intergenic | 15.0% |
| !! | AATTTATCGTTCAACTTATT+AGG | + | chr5.3:19176195-19176214 | None:intergenic | 20.0% |
| !! | GCAGTTAAAATAATAATAAG+AGG | + | chr5.3:19175915-19175934 | None:intergenic | 20.0% |
| !! | TAAAATAATAATAAGAGGAG+TGG | + | chr5.3:19175910-19175929 | None:intergenic | 20.0% |
| !! | TTATTAGGTAAAAGTTCAAT+AGG | + | chr5.3:19176180-19176199 | None:intergenic | 20.0% |
| ! | AAAGTGTGAAAGACTATATT+AGG | - | chr5.3:19176041-19176060 | MS.gene069096:intron | 25.0% |
| CTAAAATGTCAAATACTGCT+TGG | + | chr5.3:19175986-19176005 | None:intergenic | 30.0% | |
| !! | GCAATCATTTTAACAATGGT+TGG | + | chr5.3:19176137-19176156 | None:intergenic | 30.0% |
| !! | TGGTGCAATCATTTTAACAA+TGG | + | chr5.3:19176141-19176160 | None:intergenic | 30.0% |
| ATCAAACTCTTATGCAGTGA+TGG | - | chr5.3:19176408-19176427 | MS.gene069096:CDS | 35.0% | |
| GTGTTATAACTAGGACTGAA+GGG | - | chr5.3:19175819-19175838 | MS.gene069096:CDS | 35.0% | |
| GTTGGACATGTGTTATAACT+AGG | - | chr5.3:19175810-19175829 | MS.gene069096:CDS | 35.0% | |
| TAGGTAAGATCTTCATGTCA+TGG | + | chr5.3:19176161-19176180 | None:intergenic | 35.0% | |
| TGTGTTATAACTAGGACTGA+AGG | - | chr5.3:19175818-19175837 | MS.gene069096:CDS | 35.0% | |
| ! | GGCAAAGTTTTTGCTAAATG+TGG | - | chr5.3:19175851-19175870 | MS.gene069096:intron | 35.0% |
| AAACTCTTATGCAGTGATGG+TGG | - | chr5.3:19176411-19176430 | MS.gene069096:CDS | 40.0% | |
| AGTGAAACATTCTGCAAGTG+TGG | - | chr5.3:19176474-19176493 | MS.gene069096:CDS | 40.0% | |
| ATTGAGAGTAGCAGTGACAA+TGG | - | chr5.3:19176450-19176469 | MS.gene069096:CDS | 40.0% | |
| GTGAAACATTCTGCAAGTGT+GGG | - | chr5.3:19176475-19176494 | MS.gene069096:CDS | 40.0% | |
| TCACTGCTACTCTCAATAAC+TGG | + | chr5.3:19176447-19176466 | None:intergenic | 40.0% | |
| GCAAAAACTTTGCCAGCATC+AGG | + | chr5.3:19175845-19175864 | None:intergenic | 45.0% | |
| GCAATGTGTTGTGCAAGACT+GGG | - | chr5.3:19175782-19175801 | MS.gene069096:CDS | 45.0% | |
| TGTTGTGCAAGACTGGGTAA+AGG | - | chr5.3:19175788-19175807 | MS.gene069096:CDS | 45.0% | |
| GGCAATGTGTTGTGCAAGAC+TGG | - | chr5.3:19175781-19175800 | MS.gene069096:CDS | 50.0% | |
| GTGCAAGACTGGGTAAAGGT+TGG | - | chr5.3:19175792-19175811 | MS.gene069096:CDS | 50.0% | |
| !! | TATGCAGTGATGGTGGAGCT+GGG | - | chr5.3:19176418-19176437 | MS.gene069096:CDS | 50.0% |
| !! | TTATGCAGTGATGGTGGAGC+TGG | - | chr5.3:19176417-19176436 | MS.gene069096:CDS | 50.0% |
| AGGACTGAAGGGCCTGATGC+TGG | - | chr5.3:19175830-19175849 | MS.gene069096:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 19175775 | 19176495 | 19175775 | ID=MS.gene069096 |
| chr5.3 | mRNA | 19175775 | 19176495 | 19175775 | ID=MS.gene069096.t1;Parent=MS.gene069096 |
| chr5.3 | exon | 19176376 | 19176495 | 19176376 | ID=MS.gene069096.t1.exon1;Parent=MS.gene069096.t1 |
| chr5.3 | CDS | 19176376 | 19176495 | 19176376 | ID=cds.MS.gene069096.t1;Parent=MS.gene069096.t1 |
| chr5.3 | exon | 19175775 | 19175845 | 19175775 | ID=MS.gene069096.t1.exon2;Parent=MS.gene069096.t1 |
| chr5.3 | CDS | 19175775 | 19175845 | 19175775 | ID=cds.MS.gene069096.t1;Parent=MS.gene069096.t1 |
| Gene Sequence |
| Protein sequence |
