Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene069220.t1 | XP_013444865.1 | 100 | 72 | 0 | 0 | 1 | 72 | 1 | 72 | 1.40E-32 | 148.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene069220.t1 | G7KQ30 | 100.0 | 72 | 0 | 0 | 1 | 72 | 1 | 72 | 1.0e-32 | 148.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049341 | MS.gene069220 | 0.802057 | 6.72E-49 | -1.69E-46 |
| MS.gene049821 | MS.gene069220 | 0.826857 | 2.16E-54 | -1.69E-46 |
| MS.gene050237 | MS.gene069220 | 0.819544 | 1.10E-52 | -1.69E-46 |
| MS.gene050807 | MS.gene069220 | 0.81151 | 6.75E-51 | -1.69E-46 |
| MS.gene050808 | MS.gene069220 | 0.812905 | 3.35E-51 | -1.69E-46 |
| MS.gene050953 | MS.gene069220 | 0.801866 | 7.36E-49 | -1.69E-46 |
| MS.gene051241 | MS.gene069220 | 0.821816 | 3.30E-53 | -1.69E-46 |
| MS.gene051243 | MS.gene069220 | 0.842809 | 2.06E-58 | -1.69E-46 |
| MS.gene051328 | MS.gene069220 | 0.826592 | 2.49E-54 | -1.69E-46 |
| MS.gene053630 | MS.gene069220 | 0.800979 | 1.12E-48 | -1.69E-46 |
| MS.gene055623 | MS.gene069220 | 0.80058 | 1.35E-48 | -1.69E-46 |
| MS.gene055818 | MS.gene069220 | 0.807146 | 5.83E-50 | -1.69E-46 |
| MS.gene056760 | MS.gene069220 | 0.813631 | 2.32E-51 | -1.69E-46 |
| MS.gene057693 | MS.gene069220 | 0.80173 | 7.84E-49 | -1.69E-46 |
| MS.gene057705 | MS.gene069220 | 0.812118 | 4.98E-51 | -1.69E-46 |
| MS.gene058906 | MS.gene069220 | 0.811158 | 8.05E-51 | -1.69E-46 |
| MS.gene060429 | MS.gene069220 | 0.829007 | 6.55E-55 | -1.69E-46 |
| MS.gene060917 | MS.gene069220 | 0.823812 | 1.13E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene069220.t1 | MTR_8g031320 | 100.000 | 72 | 0 | 0 | 1 | 72 | 1 | 72 | 2.15e-50 | 153 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene069220.t1 | AT5G44710 | 59.722 | 72 | 29 | 0 | 1 | 72 | 1 | 72 | 6.10e-31 | 104 |
Find 16 sgRNAs with CRISPR-Local
Find 16 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGTCATCAATTGAATCCTTC+AGG | 0.310963 | 8.2:-67033701 | MS.gene069220:CDS |
| ATTCTCCGCATGAAAATGTT+TGG | 0.384414 | 8.2:-67033659 | MS.gene069220:CDS |
| TGTTGACGAGCCATAACATT+GGG | 0.413822 | 8.2:+67033590 | None:intergenic |
| TGAAGATGACCCCAATGTTA+TGG | 0.423766 | 8.2:-67033600 | MS.gene069220:CDS |
| TCTGCGCGAGCAACAATATT+CGG | 0.425555 | 8.2:-67033722 | MS.gene069220:CDS |
| GTCATCAATTGAATCCTTCA+GGG | 0.500225 | 8.2:-67033700 | MS.gene069220:CDS |
| TGAGCAGATTTCTTCCCTGA+AGG | 0.553711 | 8.2:+67033686 | None:intergenic |
| TCATCTTCATTGATATCATG+AGG | 0.573272 | 8.2:+67033614 | None:intergenic |
| ATGTTAACCTCTGCAATCAA+CGG | 0.613305 | 8.2:-67033755 | MS.gene069220:CDS |
| CAGACCTCCGTTGATTGCAG+AGG | 0.634979 | 8.2:+67033748 | None:intergenic |
| TTGTTGACGAGCCATAACAT+TGG | 0.639191 | 8.2:+67033589 | None:intergenic |
| CATCTTCATTGATATCATGA+GGG | 0.654939 | 8.2:+67033615 | None:intergenic |
| GTTGACGAGCCATAACATTG+GGG | 0.685036 | 8.2:+67033591 | None:intergenic |
| TTAACCTCTGCAATCAACGG+AGG | 0.698405 | 8.2:-67033752 | MS.gene069220:CDS |
| AAGAAGAAGATGGCTAGCGG+CGG | 0.707252 | 8.2:-67033788 | None:intergenic |
| GTAAAGAAGAAGATGGCTAG+CGG | 0.708935 | 8.2:-67033791 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| CATCTTCATTGATATCATGA+GGG | + | chr8.2:67033747-67033766 | None:intergenic | 30.0% | |
| TCATCTTCATTGATATCATG+AGG | + | chr8.2:67033748-67033767 | None:intergenic | 30.0% | |
| ! | TTTCACCAAACATTTTCATG+CGG | + | chr8.2:67033708-67033727 | None:intergenic | 30.0% |
| ATGTTAACCTCTGCAATCAA+CGG | - | chr8.2:67033604-67033623 | MS.gene069220:CDS | 35.0% | |
| ATTCTCCGCATGAAAATGTT+TGG | - | chr8.2:67033700-67033719 | MS.gene069220:CDS | 35.0% | |
| GTCATCAATTGAATCCTTCA+GGG | - | chr8.2:67033659-67033678 | MS.gene069220:CDS | 35.0% | |
| TTGGTGAAAAAGTTGCTCAA+TGG | - | chr8.2:67033719-67033738 | MS.gene069220:CDS | 35.0% | |
| GGTCATCAATTGAATCCTTC+AGG | - | chr8.2:67033658-67033677 | MS.gene069220:CDS | 40.0% | |
| TGAAGATGACCCCAATGTTA+TGG | - | chr8.2:67033759-67033778 | MS.gene069220:CDS | 40.0% | |
| TGTTGACGAGCCATAACATT+GGG | + | chr8.2:67033772-67033791 | None:intergenic | 40.0% | |
| TTGTTGACGAGCCATAACAT+TGG | + | chr8.2:67033773-67033792 | None:intergenic | 40.0% | |
| GTTGACGAGCCATAACATTG+GGG | + | chr8.2:67033771-67033790 | None:intergenic | 45.0% | |
| TCTGCGCGAGCAACAATATT+CGG | - | chr8.2:67033637-67033656 | MS.gene069220:CDS | 45.0% | |
| TGAGCAGATTTCTTCCCTGA+AGG | + | chr8.2:67033676-67033695 | None:intergenic | 45.0% | |
| TTAACCTCTGCAATCAACGG+AGG | - | chr8.2:67033607-67033626 | MS.gene069220:CDS | 45.0% | |
| ! | CAGACCTCCGTTGATTGCAG+AGG | + | chr8.2:67033614-67033633 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.2 | gene | 67033580 | 67033801 | 67033580 | ID=MS.gene069220 |
| chr8.2 | mRNA | 67033580 | 67033801 | 67033580 | ID=MS.gene069220.t1;Parent=MS.gene069220 |
| chr8.2 | exon | 67033580 | 67033801 | 67033580 | ID=MS.gene069220.t1.exon1;Parent=MS.gene069220.t1 |
| chr8.2 | CDS | 67033580 | 67033801 | 67033580 | ID=cds.MS.gene069220.t1;Parent=MS.gene069220.t1 |
| Gene Sequence |
| Protein sequence |
