AlfalfaGEDB | MS.gene069313

M. sativa cultivar XinJiangDaYe / MS.gene069313

KEGG: K02966
GO: GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0019538 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0071704 GO:0110165 GO:1901564 GO:1901566 GO:1901576
PFAM: PF01090.22

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene069313.t1	XP_004516579.1	98.9	92	1	0	1	92	29	120	2.20E-46	194.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene069313.t1	Q9SGA6	83.7	92	15	0	1	92	29	120	3.3e-43	175.3

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene069313.t1	A0A1S2Z7S6	98.9	92	1	0	1	92	29	120	1.6e-46	194.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049255	MS.gene069313	0.803599	3.23E-49	-1.69E-46
MS.gene049876	MS.gene069313	0.806683	7.30E-50	-1.69E-46
MS.gene050290	MS.gene069313	0.808463	3.06E-50	-1.69E-46
MS.gene050916	MS.gene069313	0.816114	6.53E-52	-1.69E-46
MS.gene052947	MS.gene069313	0.814454	1.53E-51	-1.69E-46
MS.gene054937	MS.gene069313	0.821711	3.49E-53	-1.69E-46
MS.gene055162	MS.gene069313	0.8064	8.38E-50	-1.69E-46
MS.gene056170	MS.gene069313	0.804498	2.10E-49	-1.69E-46
MS.gene056917	MS.gene069313	0.835266	1.86E-56	-1.69E-46
MS.gene060429	MS.gene069313	0.846909	1.61E-59	-1.69E-46
MS.gene060917	MS.gene069313	0.83818	3.35E-57	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene069313.t1	MTR_3g013635	98.913	92	1	1	92	29	120	8.54e-67	197
MS.gene069313.t1	MTR_3g013635	98.913	92	1	1	92	29	120	8.54e-67	197
MS.gene069313.t1	MTR_8g040740	92.391	92	7	1	92	29	120	3.80e-64	190

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene069313.t1	AT3G02080	83.696	92	15	1	92	29	120	8.89e-59	177
MS.gene069313.t1	AT5G15520	84.783	92	14	1	92	29	120	1.73e-58	176
MS.gene069313.t1	AT5G61170	82.609	92	16	1	92	29	120	1.79e-58	176

Find 35 sgRNAs with CRISPR-Local

Find 42 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGTGAAGACTGCAAGGTTTA+AGG	0.181111	3.3:+79478206	MS.gene069313:CDS
GGTGCATTCCAGAGGATTTA+TGG	0.279212	3.3:+79478445	MS.gene069313:CDS
TTCACAATATCTGTCCATTC+TGG	0.341460	3.3:-79478189	None:intergenic
CCTTGGTGTCCATCTCAATA+AGG	0.385015	3.3:-79478561	None:intergenic
GACTGCAAGGTTTAAGGAGT+TGG	0.416477	3.3:+79478212	MS.gene069313:CDS
ACCCCTCAAGTAGATCTTCC+TGG	0.431786	3.3:-79478410	None:intergenic
GACTTCCACCATAAATCCTC+TGG	0.453242	3.3:-79478453	None:intergenic
ATCTACTTGAGGGGTGGACT+CGG	0.454423	3.3:+79478418	MS.gene069313:CDS
GTGCCGCAGCTTCCATGGCC+AGG	0.485644	3.3:+79478392	MS.gene069313:intron
GCTCTGACGTAGTACCAGTC+AGG	0.489319	3.3:-79478246	None:intergenic
TAGTACCAGTCAGGGTCATA+AGG	0.498306	3.3:-79478237	None:intergenic
TATGGTGGAAGTCAGAGAAA+TGG	0.514768	3.3:+79478463	MS.gene069313:CDS
CAAGTAGATCTTCCTGGCCA+TGG	0.517027	3.3:-79478404	None:intergenic
GGCCAGGAAGATCTACTTGA+GGG	0.518439	3.3:+79478408	MS.gene069313:CDS
TTGAGGGGTGGACTCGGTGT+TGG	0.522669	3.3:+79478424	MS.gene069313:CDS
GATAATGAGTTCTTACCCCT+TGG	0.524570	3.3:-79478578	None:intergenic
TGGCTCCTTATGACCCTGAC+TGG	0.535373	3.3:+79478232	MS.gene069313:CDS
CCACTGCTCTTGCAGAAATG+AGG	0.542089	3.3:-79478496	None:intergenic
GACTGGTACTACGTCAGAGC+TGG	0.543992	3.3:+79478249	MS.gene069313:CDS
AACAGATGGAGCTTCCAGAA+TGG	0.551656	3.3:+79478175	None:intergenic
TTGAAGAATGTGGCGAGCAA+TGG	0.555153	3.3:-79478521	None:intergenic
GAACATGAACCTTATTGAGA+TGG	0.561257	3.3:+79478552	MS.gene069313:CDS
CCTCATTTCTGCAAGAGCAG+TGG	0.589477	3.3:+79478496	MS.gene069313:CDS
TCGGTGTTGGTGCATTCCAG+AGG	0.603387	3.3:+79478437	MS.gene069313:CDS
CAGATATTGTGAAGACTGCA+AGG	0.617924	3.3:+79478199	MS.gene069313:CDS
CTGCTCTTGCAGAAATGAGG+TGG	0.621206	3.3:-79478493	None:intergenic
AGGAAGATCTACTTGAGGGG+TGG	0.621735	3.3:+79478412	MS.gene069313:CDS
CTCTGACGTAGTACCAGTCA+GGG	0.627550	3.3:-79478245	None:intergenic
CTTATTGAGATGGACACCAA+GGG	0.649426	3.3:+79478562	MS.gene069313:CDS
TGGCCAGGAAGATCTACTTG+AGG	0.650322	3.3:+79478407	MS.gene069313:CDS
CCTTATTGAGATGGACACCA+AGG	0.671459	3.3:+79478561	MS.gene069313:CDS
GCATTCCAGAGGATTTATGG+TGG	0.673361	3.3:+79478448	MS.gene069313:CDS
TCTGCAACTGTTGAAGAATG+TGG	0.679137	3.3:-79478531	None:intergenic
GCCAGGAAGATCTACTTGAG+GGG	0.686642	3.3:+79478409	MS.gene069313:CDS
TTATTGAGATGGACACCAAG+GGG	0.704884	3.3:+79478563	MS.gene069313:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTTTTTGTTAATTTAAACAA+GGG	-	chr3.3:79478311-79478330	None:intergenic	10.0%
!!!	TTTTTTTGTTAATTTAAACA+AGG	-	chr3.3:79478312-79478331	None:intergenic	10.0%
!!	AACAAAAAAATAACTTTCGA+AGG	+	chr3.3:79478322-79478341	MS.gene069313:intron	20.0%
!!	ACAAAAAAATAACTTTCGAA+GGG	+	chr3.3:79478323-79478342	MS.gene069313:intron	20.0%
!	ACATTGCAATTTTCAACAAC+AGG	-	chr3.3:79478365-79478384	None:intergenic	30.0%
	GAACATGAACCTTATTGAGA+TGG	+	chr3.3:79478552-79478571	MS.gene069313:CDS	35.0%
	GTTAATTTAAACAAGGGACC+AGG	-	chr3.3:79478305-79478324	None:intergenic	35.0%
	TTCACAATATCTGTCCATTC+TGG	-	chr3.3:79478192-79478211	None:intergenic	35.0%
!!	GTTTGATTTTGTGCATTTCC+TGG	+	chr3.3:79478284-79478303	MS.gene069313:intron	35.0%
	CAGATATTGTGAAGACTGCA+AGG	+	chr3.3:79478199-79478218	MS.gene069313:CDS	40.0%
	CTTATTGAGATGGACACCAA+GGG	+	chr3.3:79478562-79478581	MS.gene069313:CDS	40.0%
	TCTGCAACTGTTGAAGAATG+TGG	-	chr3.3:79478534-79478553	None:intergenic	40.0%
	TGTGAAGACTGCAAGGTTTA+AGG	+	chr3.3:79478206-79478225	MS.gene069313:CDS	40.0%
	TTATTGAGATGGACACCAAG+GGG	+	chr3.3:79478563-79478582	MS.gene069313:CDS	40.0%
!	TATGGTGGAAGTCAGAGAAA+TGG	+	chr3.3:79478463-79478482	MS.gene069313:CDS	40.0%
	CCTTATTGAGATGGACACCA+AGG	+	chr3.3:79478561-79478580	MS.gene069313:CDS	45.0%
	GACTGCAAGGTTTAAGGAGT+TGG	+	chr3.3:79478212-79478231	MS.gene069313:CDS	45.0%
	GACTTCCACCATAAATCCTC+TGG	-	chr3.3:79478456-79478475	None:intergenic	45.0%
	GCATTCCAGAGGATTTATGG+TGG	+	chr3.3:79478448-79478467	MS.gene069313:CDS	45.0%
	GGTGCATTCCAGAGGATTTA+TGG	+	chr3.3:79478445-79478464	MS.gene069313:CDS	45.0%
	TAGTACCAGTCAGGGTCATA+AGG	-	chr3.3:79478240-79478259	None:intergenic	45.0%
	TTGAAGAATGTGGCGAGCAA+TGG	-	chr3.3:79478524-79478543	None:intergenic	45.0%
!!	CCTTGGTGTCCATCTCAATA+AGG	-	chr3.3:79478564-79478583	None:intergenic	45.0%
	ACCCCTCAAGTAGATCTTCC+TGG	-	chr3.3:79478413-79478432	None:intergenic	50.0%
	AGGAAGATCTACTTGAGGGG+TGG	+	chr3.3:79478412-79478431	MS.gene069313:CDS	50.0%
	CAAGTAGATCTTCCTGGCCA+TGG	-	chr3.3:79478407-79478426	None:intergenic	50.0%
	CCACTGCTCTTGCAGAAATG+AGG	-	chr3.3:79478499-79478518	None:intergenic	50.0%
	CCTCATTTCTGCAAGAGCAG+TGG	+	chr3.3:79478496-79478515	MS.gene069313:CDS	50.0%
	CTCTGACGTAGTACCAGTCA+GGG	-	chr3.3:79478248-79478267	None:intergenic	50.0%
	CTGCTCTTGCAGAAATGAGG+TGG	-	chr3.3:79478496-79478515	None:intergenic	50.0%
	GCCAGGAAGATCTACTTGAG+GGG	+	chr3.3:79478409-79478428	MS.gene069313:CDS	50.0%
	GGCCAGGAAGATCTACTTGA+GGG	+	chr3.3:79478408-79478427	MS.gene069313:CDS	50.0%
	TGGCCAGGAAGATCTACTTG+AGG	+	chr3.3:79478407-79478426	MS.gene069313:CDS	50.0%
!	ATCTACTTGAGGGGTGGACT+CGG	+	chr3.3:79478418-79478437	MS.gene069313:CDS	50.0%
!	TTTTTGTGCCGCAGCTTCCA+TGG	+	chr3.3:79478387-79478406	MS.gene069313:intron	50.0%
	GACTGGTACTACGTCAGAGC+TGG	+	chr3.3:79478249-79478268	MS.gene069313:CDS	55.0%
	GCTCTGACGTAGTACCAGTC+AGG	-	chr3.3:79478249-79478268	None:intergenic	55.0%
	TGGCTCCTTATGACCCTGAC+TGG	+	chr3.3:79478232-79478251	MS.gene069313:CDS	55.0%
!!	TCGGTGTTGGTGCATTCCAG+AGG	+	chr3.3:79478437-79478456	MS.gene069313:CDS	55.0%
	CTTCCTGGCCATGGAAGCTG+CGG	-	chr3.3:79478398-79478417	None:intergenic	60.0%
!!	TTGAGGGGTGGACTCGGTGT+TGG	+	chr3.3:79478424-79478443	MS.gene069313:CDS	60.0%
	GTGCCGCAGCTTCCATGGCC+AGG	+	chr3.3:79478392-79478411	MS.gene069313:intron	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.3	gene	79478180	79478588	79478180	ID=MS.gene069313
chr3.3	mRNA	79478180	79478588	79478180	ID=MS.gene069313.t1;Parent=MS.gene069313
chr3.3	exon	79478180	79478270	79478180	ID=MS.gene069313.t1.exon1;Parent=MS.gene069313.t1
chr3.3	CDS	79478180	79478270	79478180	ID=cds.MS.gene069313.t1;Parent=MS.gene069313.t1
chr3.3	exon	79478401	79478588	79478401	ID=MS.gene069313.t1.exon2;Parent=MS.gene069313.t1
chr3.3	CDS	79478401	79478588	79478401	ID=cds.MS.gene069313.t1;Parent=MS.gene069313.t1

Gene Sequence

>MS.gene069313

ATGGAGCTTCCAGAATGGACAGATATTGTGAAGACTGCAAGGTTTAAGGAGTTGGCTCCTTATGACCCTGACTGGTACTACGTCAGAGCTGGTGAGTTTCTTGTGTTTGATTTTGTGCATTTCCTGGTCCCTTGTTTAAATTAACAAAAAAATAACTTTCGAAGGGAACATTTTTTGATCAACCTGTTGTTGAAAATTGCAATGTGTTTTTTGTGCCGCAGCTTCCATGGCCAGGAAGATCTACTTGAGGGGTGGACTCGGTGTTGGTGCATTCCAGAGGATTTATGGTGGAAGTCAGAGAAATGGCAGTCGTCCACCTCATTTCTGCAAGAGCAGTGGTTCCATTGCTCGCCACATTCTTCAACAGTTGCAGAACATGAACCTTATTGAGATGGACACCAAGGGGTAA

Protein sequence

>MS.gene069313.t1

MELPEWTDIVKTARFKELAPYDPDWYYVRAASMARKIYLRGGLGVGAFQRIYGGSQRNGSRPPHFCKSSGSIARHILQQLQNMNLIEMDTKG