Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07116.t1 | XP_003625904.1 | 98.6 | 293 | 4 | 0 | 1 | 293 | 1 | 293 | 1.90E-157 | 565.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07116.t1 | Q9LHH7 | 78.7 | 287 | 61 | 0 | 2 | 288 | 9 | 295 | 1.2e-128 | 460.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07116.t1 | B7FKU9 | 98.6 | 293 | 4 | 0 | 1 | 293 | 1 | 293 | 1.4e-157 | 565.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050825 | MS.gene07116 | 0.837312 | 5.60E-57 | -1.69E-46 |
| MS.gene05087 | MS.gene07116 | 0.838554 | 2.68E-57 | -1.69E-46 |
| MS.gene055634 | MS.gene07116 | 0.808971 | 2.38E-50 | -1.69E-46 |
| MS.gene057684 | MS.gene07116 | 0.835787 | 1.37E-56 | -1.69E-46 |
| MS.gene058719 | MS.gene07116 | 0.828752 | 7.55E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene07116 | MS.gene023835 | PPI |
| MS.gene27688 | MS.gene07116 | PPI |
| MS.gene07116 | MS.gene62999 | PPI |
| MS.gene33587 | MS.gene07116 | PPI |
| MS.gene65953 | MS.gene07116 | PPI |
| MS.gene07116 | MS.gene026617 | PPI |
| MS.gene52204 | MS.gene07116 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07116.t1 | MTR_7g108580 | 98.635 | 293 | 4 | 0 | 1 | 293 | 1 | 293 | 0.0 | 585 |
| MS.gene07116.t1 | MTR_7g076680 | 66.316 | 285 | 96 | 0 | 4 | 288 | 62 | 346 | 2.57e-141 | 402 |
| MS.gene07116.t1 | MTR_7g076680 | 66.316 | 285 | 96 | 0 | 4 | 288 | 58 | 342 | 2.69e-141 | 402 |
| MS.gene07116.t1 | MTR_7g076680 | 66.316 | 285 | 96 | 0 | 4 | 288 | 79 | 363 | 3.32e-141 | 402 |
| MS.gene07116.t1 | MTR_1g030450 | 62.021 | 287 | 109 | 0 | 2 | 288 | 70 | 356 | 8.09e-127 | 365 |
| MS.gene07116.t1 | MTR_3g007490 | 51.211 | 289 | 140 | 1 | 2 | 290 | 73 | 360 | 7.20e-106 | 312 |
| MS.gene07116.t1 | MTR_3g007580 | 43.750 | 112 | 56 | 2 | 127 | 237 | 13 | 118 | 1.44e-25 | 99.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07116.t1 | AT3G12290 | 78.746 | 287 | 61 | 0 | 2 | 288 | 9 | 295 | 5.58e-170 | 473 |
| MS.gene07116.t1 | AT4G00620 | 65.157 | 287 | 100 | 0 | 2 | 288 | 69 | 355 | 3.07e-139 | 397 |
| MS.gene07116.t1 | AT2G38660 | 61.404 | 285 | 110 | 0 | 4 | 288 | 65 | 349 | 1.26e-125 | 362 |
| MS.gene07116.t1 | AT2G38660 | 61.404 | 285 | 110 | 0 | 4 | 288 | 65 | 349 | 1.26e-125 | 362 |
| MS.gene07116.t1 | AT2G38660 | 59.289 | 253 | 103 | 0 | 4 | 256 | 65 | 317 | 8.48e-107 | 314 |
| MS.gene07116.t1 | AT4G00600 | 54.355 | 287 | 95 | 1 | 2 | 288 | 55 | 305 | 1.65e-105 | 310 |
| MS.gene07116.t1 | AT2G38660 | 59.887 | 177 | 71 | 0 | 4 | 180 | 65 | 241 | 2.34e-73 | 226 |
| MS.gene07116.t1 | AT2G38660 | 49.495 | 99 | 50 | 0 | 4 | 102 | 65 | 163 | 2.17e-26 | 102 |
| MS.gene07116.t1 | AT2G38660 | 49.495 | 99 | 50 | 0 | 4 | 102 | 65 | 163 | 2.17e-26 | 102 |
| MS.gene07116.t1 | AT2G38660 | 49.495 | 99 | 50 | 0 | 4 | 102 | 65 | 163 | 2.17e-26 | 102 |
| MS.gene07116.t1 | AT2G38660 | 49.495 | 99 | 50 | 0 | 4 | 102 | 65 | 163 | 2.17e-26 | 102 |
Find 74 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTCTCTTCATTTATATGTT+TGG | 0.237367 | 7.1:+5178225 | None:intergenic |
| TTCTCTTCATTTATATGTTT+GGG | 0.275577 | 7.1:+5178226 | None:intergenic |
| GAGAAAAGCTTGCGCTGATT+TGG | 0.292482 | 7.1:-5178461 | MS.gene07116:CDS |
| TATCATCATCTTCAGGTTGC+TGG | 0.330920 | 7.1:-5178541 | MS.gene07116:intron |
| GGTTGGATTACTCCTGTTCC+TGG | 0.334532 | 7.1:-5171674 | MS.gene07116:CDS |
| ACGAAGTGGTGTAAGTATAA+AGG | 0.338275 | 7.1:-5172506 | MS.gene07116:CDS |
| AGAAAAGCTTGCGCTGATTT+GGG | 0.341343 | 7.1:-5178460 | MS.gene07116:CDS |
| AGCAACATTGTGACTGTCAT+TGG | 0.347400 | 7.1:+5171641 | None:intergenic |
| CAACTAGGAAGGCAGGTTAT+AGG | 0.352622 | 7.1:-5171739 | MS.gene07116:CDS |
| GAGGAAGCATCTAAAGTTGC+TGG | 0.352957 | 7.1:-5171695 | MS.gene07116:CDS |
| CGAAGTGGTGTAAGTATAAA+GGG | 0.365346 | 7.1:-5172505 | MS.gene07116:CDS |
| TCAGCTTCGGAAATTTGTTC+AGG | 0.386703 | 7.1:+5178412 | None:intergenic |
| TTCAAAAGCAGTAATGAAAC+TGG | 0.393159 | 7.1:+5172439 | None:intergenic |
| AGCATTTACTTTGGGAGTGC+AGG | 0.399690 | 7.1:+5178105 | None:intergenic |
| TTATATGTGTGCAGGTATAT+TGG | 0.405081 | 7.1:-5178263 | MS.gene07116:intron |
| GAGAGGGTCTCTGCCTTTCA+TGG | 0.418461 | 7.1:+5178135 | None:intergenic |
| AAGGCAGGTTATAGGCTTGT+TGG | 0.427901 | 7.1:-5171731 | MS.gene07116:CDS |
| TATAACCTGCCTTCCTAGTT+GGG | 0.428149 | 7.1:+5171741 | None:intergenic |
| CTATAACCTGCCTTCCTAGT+TGG | 0.431628 | 7.1:+5171740 | None:intergenic |
| GCTTCACGTATGATATCTTC+TGG | 0.435944 | 7.1:+5172373 | None:intergenic |
| ATGCTTGATAACTTCAGCTT+CGG | 0.440233 | 7.1:+5178399 | None:intergenic |
| TTTCTTAGATCAAGGGAAGT+TGG | 0.440672 | 7.1:-5171814 | MS.gene07116:intron |
| GTGTAAGTATAAAGGGGAAA+AGG | 0.444068 | 7.1:-5172498 | MS.gene07116:CDS |
| AATGTTGCTGAAGAATACTT+TGG | 0.453034 | 7.1:-5171627 | MS.gene07116:CDS |
| GGACCTACACCTCCAGGAAC+AGG | 0.460733 | 7.1:+5171662 | None:intergenic |
| GGACTTGCAGTGGTGATAGT+AGG | 0.466821 | 7.1:-5178520 | MS.gene07116:CDS |
| AGCCTTGAGAAGGATGTTGA+TGG | 0.475914 | 7.1:-5178187 | MS.gene07116:CDS |
| CGTATCCTCTCTGAGAAATA+CGG | 0.476330 | 7.1:-5178648 | MS.gene07116:CDS |
| AGTGACTCACCATGTACATT+GGG | 0.481285 | 7.1:+5178355 | None:intergenic |
| GATATTGTTATTGCAGCAGC+AGG | 0.488835 | 7.1:-5172349 | MS.gene07116:CDS |
| ACATCTATTACTGCAGCTCC+AGG | 0.493139 | 7.1:+5171785 | None:intergenic |
| ACTCCTGTTCCTGGAGGTGT+AGG | 0.496506 | 7.1:-5171665 | MS.gene07116:CDS |
| TGTTATTGCAGCAGCAGGAC+AGG | 0.498986 | 7.1:-5172344 | MS.gene07116:CDS |
| CGTATTTCTCAGAGAGGATA+CGG | 0.500459 | 7.1:+5178649 | None:intergenic |
| ATCGGCAAGCTTGCCATGAA+AGG | 0.510148 | 7.1:-5178148 | MS.gene07116:CDS |
| GGCTTTCATCCTTTGAACAT+CGG | 0.514273 | 7.1:-5178166 | MS.gene07116:CDS |
| TCATTTATATGTTTGGGCAG+TGG | 0.514441 | 7.1:+5178232 | None:intergenic |
| AACTGAAATCAGCCTTGAGA+AGG | 0.521126 | 7.1:-5178197 | MS.gene07116:CDS |
| TGTAAGTATAAAGGGGAAAA+GGG | 0.521694 | 7.1:-5172497 | MS.gene07116:CDS |
| GACTTGCAGTGGTGATAGTA+GGG | 0.525562 | 7.1:-5178519 | MS.gene07116:CDS |
| GGGAAAAGGGCAGTTGTGGT+TGG | 0.536435 | 7.1:-5172484 | MS.gene07116:CDS |
| TTGGTAGAAGCAACATAGTC+GGG | 0.537576 | 7.1:-5172465 | MS.gene07116:CDS |
| GCATTTACTTTGGGAGTGCA+GGG | 0.540237 | 7.1:+5178106 | None:intergenic |
| AGCAGCAGGACAGGCAAAGA+TGG | 0.543320 | 7.1:-5172335 | MS.gene07116:intron |
| GCAAGCTTGCCGATGTTCAA+AGG | 0.547348 | 7.1:+5178157 | None:intergenic |
| CTTCGGAAATTTGTTCAGGA+AGG | 0.547633 | 7.1:+5178416 | None:intergenic |
| AGATCGGATTGTTTGTGCCA+CGG | 0.550515 | 7.1:+5178689 | None:intergenic |
| GATGACCCAACTAGGAAGGC+AGG | 0.556133 | 7.1:-5171746 | MS.gene07116:CDS |
| AAGCATCTAAAGTTGCTGGT+TGG | 0.557994 | 7.1:-5171691 | MS.gene07116:CDS |
| ATTCTGTTGATGACCCAACT+AGG | 0.559417 | 7.1:-5171754 | MS.gene07116:CDS |
| GTCATTGGACCTACACCTCC+AGG | 0.563478 | 7.1:+5171656 | None:intergenic |
| AAGTGACTCACCATGTACAT+TGG | 0.565737 | 7.1:+5178354 | None:intergenic |
| AAAGGGGAAAAGGGCAGTTG+TGG | 0.567603 | 7.1:-5172488 | MS.gene07116:CDS |
| TCAGGTTGCTGGACTTGCAG+TGG | 0.572590 | 7.1:-5178530 | MS.gene07116:intron |
| GTTGGTAGAAGCAACATAGT+CGG | 0.574369 | 7.1:-5172466 | MS.gene07116:CDS |
| CAAATGGCCACAGTAATTGA+CGG | 0.583278 | 7.1:-5178717 | MS.gene07116:CDS |
| AGCCATCAACATCCTTCTCA+AGG | 0.583414 | 7.1:+5178185 | None:intergenic |
| CCTCGTCGGCGATTTCAGAT+CGG | 0.583482 | 7.1:+5178673 | None:intergenic |
| AAGGGAAGTTGGATAAAACC+TGG | 0.587815 | 7.1:-5171803 | MS.gene07116:CDS |
| TGTTGATGACCCAACTAGGA+AGG | 0.598349 | 7.1:-5171750 | MS.gene07116:CDS |
| GGAGTGCAGGGAACAAAGAG+AGG | 0.599700 | 7.1:+5178118 | None:intergenic |
| GGAGCTGCAGTAATAGATGT+TGG | 0.599760 | 7.1:-5171782 | MS.gene07116:CDS |
| AACGCTGATCCCAATGTACA+TGG | 0.616668 | 7.1:-5178364 | MS.gene07116:intron |
| GGCTTTGCCGTCAATTACTG+TGG | 0.622113 | 7.1:+5178710 | None:intergenic |
| CCGATCTGAAATCGCCGACG+AGG | 0.637149 | 7.1:-5178673 | MS.gene07116:CDS |
| TGGATTACTCCTGTTCCTGG+AGG | 0.641207 | 7.1:-5171671 | MS.gene07116:CDS |
| CCTTGCCGTATTTCTCAGAG+AGG | 0.642605 | 7.1:+5178643 | None:intergenic |
| CCTCTCTGAGAAATACGGCA+AGG | 0.642652 | 7.1:-5178643 | MS.gene07116:intron |
| AGTAATTGACGGCAAAGCCG+TGG | 0.650301 | 7.1:-5178706 | MS.gene07116:CDS |
| GAAGTGGTGTAAGTATAAAG+GGG | 0.666613 | 7.1:-5172504 | MS.gene07116:CDS |
| ACTTGTGTGTGAATGAGCAA+TGG | 0.673957 | 7.1:+5172399 | None:intergenic |
| AGAGAGGATACGGACCTCGT+CGG | 0.680772 | 7.1:+5178659 | None:intergenic |
| GAGTGCAGGGAACAAAGAGA+GGG | 0.687238 | 7.1:+5178119 | None:intergenic |
| CTTGAACTATTGTCACGAAG+TGG | 0.690195 | 7.1:-5172520 | MS.gene07116:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 5171592 | 5178736 | 5171592 | ID=MS.gene07116 |
| chr7.1 | mRNA | 5171592 | 5178736 | 5171592 | ID=MS.gene07116.t1;Parent=MS.gene07116 |
| chr7.1 | exon | 5178644 | 5178736 | 5178644 | ID=MS.gene07116.t1.exon1;Parent=MS.gene07116.t1 |
| chr7.1 | CDS | 5178644 | 5178736 | 5178644 | ID=cds.MS.gene07116.t1;Parent=MS.gene07116.t1 |
| chr7.1 | exon | 5178365 | 5178548 | 5178365 | ID=MS.gene07116.t1.exon2;Parent=MS.gene07116.t1 |
| chr7.1 | CDS | 5178365 | 5178548 | 5178365 | ID=cds.MS.gene07116.t1;Parent=MS.gene07116.t1 |
| chr7.1 | exon | 5178114 | 5178271 | 5178114 | ID=MS.gene07116.t1.exon3;Parent=MS.gene07116.t1 |
| chr7.1 | CDS | 5178114 | 5178271 | 5178114 | ID=cds.MS.gene07116.t1;Parent=MS.gene07116.t1 |
| chr7.1 | exon | 5172336 | 5172548 | 5172336 | ID=MS.gene07116.t1.exon4;Parent=MS.gene07116.t1 |
| chr7.1 | CDS | 5172336 | 5172548 | 5172336 | ID=cds.MS.gene07116.t1;Parent=MS.gene07116.t1 |
| chr7.1 | exon | 5171592 | 5171828 | 5171592 | ID=MS.gene07116.t1.exon5;Parent=MS.gene07116.t1 |
| chr7.1 | CDS | 5171592 | 5171828 | 5171592 | ID=cds.MS.gene07116.t1;Parent=MS.gene07116.t1 |
| Gene Sequence |
| Protein sequence |
