Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071230.t1 | XP_003616676.2 | 96.7 | 520 | 16 | 1 | 1 | 519 | 1 | 520 | 5.80E-274 | 953 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071230.t1 | Q8BGH2 | 27.0 | 456 | 276 | 17 | 54 | 493 | 30 | 444 | 1.7e-27 | 125.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071230.t1 | G7K274 | 96.7 | 520 | 16 | 1 | 1 | 519 | 1 | 520 | 4.2e-274 | 953.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049166 | MS.gene071230 | 0.80385 | 2.86E-49 | -1.69E-46 |
| MS.gene04984 | MS.gene071230 | 0.805576 | 1.25E-49 | -1.69E-46 |
| MS.gene050084 | MS.gene071230 | 0.804799 | 1.82E-49 | -1.69E-46 |
| MS.gene050085 | MS.gene071230 | 0.816428 | 5.56E-52 | -1.69E-46 |
| MS.gene050114 | MS.gene071230 | 0.8571 | 2.05E-62 | -1.69E-46 |
| MS.gene050317 | MS.gene071230 | 0.814445 | 1.53E-51 | -1.69E-46 |
| MS.gene050838 | MS.gene071230 | 0.821121 | 4.78E-53 | -1.69E-46 |
| MS.gene052342 | MS.gene071230 | 0.80077 | 1.23E-48 | -1.69E-46 |
| MS.gene052397 | MS.gene071230 | 0.802398 | 5.72E-49 | -1.69E-46 |
| MS.gene052434 | MS.gene071230 | 0.803285 | 3.75E-49 | -1.69E-46 |
| MS.gene053381 | MS.gene071230 | 0.837843 | 4.09E-57 | -1.69E-46 |
| MS.gene054823 | MS.gene071230 | 0.823387 | 1.42E-53 | -1.69E-46 |
| MS.gene055441 | MS.gene071230 | 0.80293 | 4.44E-49 | -1.69E-46 |
| MS.gene056097 | MS.gene071230 | 0.807189 | 5.71E-50 | -1.69E-46 |
| MS.gene056181 | MS.gene071230 | 0.800686 | 1.28E-48 | -1.69E-46 |
| MS.gene056432 | MS.gene071230 | 0.811463 | 6.92E-51 | -1.69E-46 |
| MS.gene056580 | MS.gene071230 | 0.810951 | 8.93E-51 | -1.69E-46 |
| MS.gene057057 | MS.gene071230 | 0.800109 | 1.68E-48 | -1.69E-46 |
| MS.gene058054 | MS.gene071230 | 0.816923 | 4.31E-52 | -1.69E-46 |
| MS.gene058638 | MS.gene071230 | 0.821408 | 4.10E-53 | -1.69E-46 |
| MS.gene058639 | MS.gene071230 | 0.831726 | 1.42E-55 | -1.69E-46 |
| MS.gene058646 | MS.gene071230 | 0.828561 | 8.40E-55 | -1.69E-46 |
| MS.gene058654 | MS.gene071230 | 0.830681 | 2.56E-55 | -1.69E-46 |
| MS.gene058758 | MS.gene071230 | 0.825597 | 4.30E-54 | -1.69E-46 |
| MS.gene059171 | MS.gene071230 | 0.802196 | 6.29E-49 | -1.69E-46 |
| MS.gene060084 | MS.gene071230 | 0.815395 | 9.45E-52 | -1.69E-46 |
| MS.gene06010 | MS.gene071230 | 0.816973 | 4.20E-52 | -1.69E-46 |
| MS.gene060296 | MS.gene071230 | 0.82128 | 4.39E-53 | -1.69E-46 |
| MS.gene060637 | MS.gene071230 | 0.807395 | 5.16E-50 | -1.69E-46 |
| MS.gene060869 | MS.gene071230 | 0.808028 | 3.79E-50 | -1.69E-46 |
| MS.gene060870 | MS.gene071230 | 0.811124 | 8.19E-51 | -1.69E-46 |
| MS.gene061006 | MS.gene071230 | 0.807156 | 5.80E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071230.t1 | MTR_5g083050 | 96.929 | 521 | 13 | 2 | 1 | 519 | 1 | 520 | 0.0 | 1040 |
| MS.gene071230.t1 | MTR_3g071450 | 75.000 | 480 | 115 | 1 | 42 | 516 | 36 | 515 | 0.0 | 759 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071230.t1 | AT5G05520 | 54.406 | 522 | 229 | 3 | 1 | 514 | 1 | 521 | 0.0 | 604 |
| MS.gene071230.t1 | AT3G11070 | 57.113 | 478 | 200 | 1 | 38 | 515 | 46 | 518 | 0.0 | 603 |
Find 123 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAGGAGGACCAAGAACTTTA+TGG | 0.213542 | 5.1:-65355461 | MS.gene071230:CDS |
| TGATCTATGGGATGCTTCTT+TGG | 0.223236 | 5.1:-65360023 | MS.gene071230:CDS |
| TGGGATGCTTCTTTGGCTTA+TGG | 0.239702 | 5.1:-65360016 | MS.gene071230:CDS |
| GTTCGCTTTGCTGTCCCTTT+TGG | 0.240210 | 5.1:-65355618 | MS.gene071230:CDS |
| CCACCCAAATAAAACCTTTC+GGG | 0.259264 | 5.1:+65355507 | None:intergenic |
| TTCCATGGGGCCAAGGGTTT+AGG | 0.262516 | 5.1:-65355548 | MS.gene071230:CDS |
| CATGCTTTCTCAAGATTTGC+AGG | 0.268705 | 5.1:-65359909 | MS.gene071230:CDS |
| TTGAATCCCCATAAAGTTCT+TGG | 0.295180 | 5.1:+65355453 | None:intergenic |
| TTGTTCTCTCAACCACCTAA+TGG | 0.305653 | 5.1:+65355305 | None:intergenic |
| GATTATCCAATCTTTGGTTT+TGG | 0.310170 | 5.1:+65361226 | None:intergenic |
| TTCAGCTTCTCAGCTCCTTT+TGG | 0.318841 | 5.1:-65354488 | MS.gene071230:CDS |
| AGCTTCGATTATCCAATCTT+TGG | 0.330408 | 5.1:+65361220 | None:intergenic |
| AACACGTCGTGGACTCGAAT+TGG | 0.332181 | 5.1:+65361263 | None:intergenic |
| ATAGGAGAGATTCACGTATT+AGG | 0.353642 | 5.1:-65359700 | MS.gene071230:CDS |
| AGTAAGCTTACTGGTGGATT+TGG | 0.356992 | 5.1:-65361020 | MS.gene071230:CDS |
| GCAGTATTCGGCAACTCTTC+CGG | 0.365264 | 5.1:+65361074 | None:intergenic |
| CTTTATGGGGATTCAAAACT+AGG | 0.366748 | 5.1:-65355446 | MS.gene071230:CDS |
| AGGAAGGATGAGCATGATAG+TGG | 0.369315 | 5.1:-65354527 | MS.gene071230:CDS |
| TTCATCGTCGATGTTGTTGT+TGG | 0.370292 | 5.1:+65361433 | None:intergenic |
| AAATGGCATCAAGGTCTATA+AGG | 0.370900 | 5.1:-65359775 | MS.gene071230:CDS |
| TTCTCTCCAGTTTGCACCCT+AGG | 0.376195 | 5.1:-65355480 | MS.gene071230:CDS |
| AAGCGACAGAGGCGAACTTT+GGG | 0.380334 | 5.1:-65359985 | MS.gene071230:CDS |
| ATGTTGTTGTTGGGTTTAGA+TGG | 0.384943 | 5.1:+65361443 | None:intergenic |
| GATGACACCCTTGACTTCAT+TGG | 0.390243 | 5.1:-65355375 | MS.gene071230:CDS |
| ATAGATCACCATAACCAAAA+AGG | 0.391033 | 5.1:+65360038 | None:intergenic |
| ACCACCCAAATAAAACCTTT+CGG | 0.391878 | 5.1:+65355506 | None:intergenic |
| TCTTACAAAGAGCAATTGTT+AGG | 0.395453 | 5.1:-65359878 | MS.gene071230:CDS |
| CAAGCGACAGAGGCGAACTT+TGG | 0.403540 | 5.1:-65359986 | MS.gene071230:CDS |
| GTCCACGACGTGTTCATAAC+CGG | 0.406066 | 5.1:-65361254 | MS.gene071230:CDS |
| AGGAGGACCAAGAACTTTAT+GGG | 0.408479 | 5.1:-65355460 | MS.gene071230:CDS |
| GAGCTTGCGGTTTCTGCGCC+AGG | 0.409872 | 5.1:-65359615 | MS.gene071230:intron |
| AATATACATTTAAATATGAT+AGG | 0.413099 | 5.1:-65359718 | MS.gene071230:CDS |
| CTGAACCTTGGCATATCTGC+TGG | 0.413852 | 5.1:-65355579 | MS.gene071230:CDS |
| ACAACAACATTAGCAGTATT+CGG | 0.423021 | 5.1:+65361062 | None:intergenic |
| GGTCTATAAGGAGGCAGCTT+GGG | 0.425603 | 5.1:-65359763 | MS.gene071230:CDS |
| AACCGCAAGCTCTGGTGATT+CGG | 0.433084 | 5.1:+65359626 | None:intergenic |
| GGCGCAGAAACCGCAAGCTC+TGG | 0.435555 | 5.1:+65359618 | None:intergenic |
| ATAATCGAAGCTGAACTTGA+TGG | 0.436592 | 5.1:-65361209 | MS.gene071230:CDS |
| AACCTTTCGGGGAGGGGAGA+TGG | 0.437309 | 5.1:+65355519 | None:intergenic |
| GTTTCCACAACTCACTTTGG+TGG | 0.439915 | 5.1:-65359656 | MS.gene071230:CDS |
| TCATCGTCGATGTTGTTGTT+GGG | 0.440858 | 5.1:+65361434 | None:intergenic |
| CAGGACTTGGTGTACACTCT+TGG | 0.442382 | 5.1:-65359824 | MS.gene071230:CDS |
| GAAACGTTGATTGCGCAGTT+TGG | 0.443169 | 5.1:+65361328 | None:intergenic |
| TAATGGGAAGATCAAAAGAT+AGG | 0.443585 | 5.1:+65355322 | None:intergenic |
| TGTTCTCTCAACCACCTAAT+GGG | 0.444160 | 5.1:+65355306 | None:intergenic |
| GACTCGAATTGGAACTTGTT+CGG | 0.446082 | 5.1:+65361274 | None:intergenic |
| TTTCGGTTGTCTGCTGGATG+TGG | 0.451957 | 5.1:-65355186 | MS.gene071230:CDS |
| GAGACGGAGAGTAAGCTTAC+TGG | 0.453210 | 5.1:-65361029 | MS.gene071230:CDS |
| ATAAAGTTCTTGGTCCTCCT+AGG | 0.454183 | 5.1:+65355463 | None:intergenic |
| GCTGTATTATAAAATCCAAA+AGG | 0.455241 | 5.1:+65355603 | None:intergenic |
| TTTGTTTCCACAACTCACTT+TGG | 0.464490 | 5.1:-65359659 | MS.gene071230:CDS |
| TGAACCTTGGCATATCTGCT+GGG | 0.468095 | 5.1:-65355578 | MS.gene071230:CDS |
| GAAACACCAAAACCAAAGAT+TGG | 0.468959 | 5.1:-65361232 | MS.gene071230:CDS |
| GAGCATGATAGTGGGAAGAC+TGG | 0.480419 | 5.1:-65354518 | MS.gene071230:CDS |
| AAATAAAACCTTTCGGGGAG+GGG | 0.482085 | 5.1:+65355513 | None:intergenic |
| CATTTGTGATGGATCTGTTA+AGG | 0.486537 | 5.1:+65359793 | None:intergenic |
| CTTAACAGATCCATCACAAA+TGG | 0.487392 | 5.1:-65359792 | MS.gene071230:CDS |
| ATCGTCAAAGCACCAGGACT+TGG | 0.488454 | 5.1:-65359837 | MS.gene071230:CDS |
| TGTAGGGTTAAGCTTGAAGC+TGG | 0.488985 | 5.1:-65361098 | MS.gene071230:CDS |
| GTCCTAAACCCTTGGCCCCA+TGG | 0.495790 | 5.1:+65355546 | None:intergenic |
| ATAAGGAGGCAGCTTGGGCA+TGG | 0.498800 | 5.1:-65359758 | MS.gene071230:CDS |
| AAAGAGTCTAGGAACTTTCG+AGG | 0.503011 | 5.1:+65355207 | None:intergenic |
| GGTCCTCCTAGGGTGCAAAC+TGG | 0.508678 | 5.1:+65355474 | None:intergenic |
| TGCGAGGCTTCAACGGCTTG+AGG | 0.510882 | 5.1:-65361132 | MS.gene071230:CDS |
| ATGAGAACTCAGCTGCCAAA+AGG | 0.512958 | 5.1:+65354473 | None:intergenic |
| CACCGAATCACCAGAGCTTG+CGG | 0.516887 | 5.1:-65359628 | MS.gene071230:CDS |
| TAAAGTTCTTGGTCCTCCTA+GGG | 0.520203 | 5.1:+65355464 | None:intergenic |
| TCTAGGCGGAAGAGTCTAGT+AGG | 0.522923 | 5.1:+65355153 | None:intergenic |
| CAAATAAAACCTTTCGGGGA+GGG | 0.524452 | 5.1:+65355512 | None:intergenic |
| GACCTTGATGCCATTTGTGA+TGG | 0.532843 | 5.1:+65359782 | None:intergenic |
| TTTATGGGGATTCAAAACTA+GGG | 0.535376 | 5.1:-65355445 | MS.gene071230:CDS |
| AGGTCTATAAGGAGGCAGCT+TGG | 0.535390 | 5.1:-65359764 | MS.gene071230:CDS |
| GGTTAAGCTTGAAGCTGGAC+CGG | 0.539261 | 5.1:-65361093 | MS.gene071230:CDS |
| TGTTAACTCCACTTGTGGCA+CGG | 0.541802 | 5.1:-65359937 | MS.gene071230:CDS |
| ATCCATCACAAATGGCATCA+AGG | 0.543631 | 5.1:-65359784 | MS.gene071230:CDS |
| TTGCTGCTGCGAGGCTTCAA+CGG | 0.545021 | 5.1:-65361139 | MS.gene071230:CDS |
| TCACGTATTAGGCCTACAAA+AGG | 0.545769 | 5.1:-65359689 | MS.gene071230:CDS |
| CATGAGTTCCTGCATTGTAG+TGG | 0.549340 | 5.1:+65361175 | None:intergenic |
| CTGTATTATAAAATCCAAAA+GGG | 0.550990 | 5.1:+65355604 | None:intergenic |
| TATAATACAGCACTGAACCT+TGG | 0.551314 | 5.1:-65355591 | MS.gene071230:CDS |
| GGATTCAAAACTAGGGGAGT+AGG | 0.552285 | 5.1:-65355438 | MS.gene071230:CDS |
| ATGGCTTAGTCCTAAACCCT+TGG | 0.553158 | 5.1:+65355538 | None:intergenic |
| TGGTGTTTATATGAAACCCT+CGG | 0.554497 | 5.1:-65361000 | MS.gene071230:intron |
| TTGTTCGGAGGCTAATCGAC+GGG | 0.555091 | 5.1:+65361289 | None:intergenic |
| CTTGATATCGTCAAAGCACC+AGG | 0.558447 | 5.1:-65359843 | MS.gene071230:CDS |
| GTATATGCTCCTCGTCTGAA+AGG | 0.558993 | 5.1:-65359962 | MS.gene071230:CDS |
| TGGCGGCAAGCAAGCGACAG+AGG | 0.567213 | 5.1:-65359996 | MS.gene071230:CDS |
| CAAGAGTGTACACCAAGTCC+TGG | 0.569597 | 5.1:+65359825 | None:intergenic |
| ACTTGGTGTACACTCTTGGA+TGG | 0.574272 | 5.1:-65359820 | MS.gene071230:CDS |
| GATCACCTCCAATGAAGTCA+AGG | 0.579571 | 5.1:+65355367 | None:intergenic |
| CGAGAAAGCCACTACAATGC+AGG | 0.579571 | 5.1:-65361183 | MS.gene071230:CDS |
| TCTCCAGTTTGCACCCTAGG+AGG | 0.582476 | 5.1:-65355477 | MS.gene071230:CDS |
| TTGAGAGAACAAGGAATACA+TGG | 0.582554 | 5.1:-65355294 | MS.gene071230:CDS |
| AGCCATCTCCCCTCCCCGAA+AGG | 0.584565 | 5.1:-65355521 | MS.gene071230:CDS |
| ATGAACAACCGTGCCACAAG+TGG | 0.585342 | 5.1:+65359929 | None:intergenic |
| TAAGGAGGCAGCTTGGGCAT+GGG | 0.585441 | 5.1:-65359757 | MS.gene071230:CDS |
| ATTAGGTGGTTGAGAGAACA+AGG | 0.591529 | 5.1:-65355303 | MS.gene071230:CDS |
| GACACCCTTGACTTCATTGG+AGG | 0.594643 | 5.1:-65355372 | MS.gene071230:CDS |
| CAGACAACCGAAAAGAGTCT+AGG | 0.595816 | 5.1:+65355196 | None:intergenic |
| TACTAGACTCTTCCGCCTAG+AGG | 0.598676 | 5.1:-65355151 | MS.gene071230:intron |
| TGTTGTTGATGTTGTTGAGA+CGG | 0.602109 | 5.1:-65361045 | MS.gene071230:CDS |
| GGAAGGATGAGCATGATAGT+GGG | 0.604644 | 5.1:-65354526 | MS.gene071230:CDS |
| GTTAAGGCATGGACTACAGA+AGG | 0.605360 | 5.1:-65360079 | MS.gene071230:CDS |
| AGGATTGTTAACTCCACTTG+TGG | 0.606581 | 5.1:-65359942 | MS.gene071230:CDS |
| CTTCTCAGATTGCTGCTGCG+AGG | 0.615777 | 5.1:-65361148 | MS.gene071230:CDS |
| GAACCTTGGCATATCTGCTG+GGG | 0.619177 | 5.1:-65355577 | MS.gene071230:CDS |
| TTATGGGGATTCAAAACTAG+GGG | 0.620512 | 5.1:-65355444 | MS.gene071230:CDS |
| CCAAATAAAACCTTTCGGGG+AGG | 0.620795 | 5.1:+65355511 | None:intergenic |
| CAGCCCCAGCAGATATGCCA+AGG | 0.628189 | 5.1:+65355574 | None:intergenic |
| GGAGGACCAAGAACTTTATG+GGG | 0.629896 | 5.1:-65355459 | MS.gene071230:CDS |
| ACGGAGAGTAAGCTTACTGG+TGG | 0.633045 | 5.1:-65361026 | MS.gene071230:CDS |
| CACCCAAATAAAACCTTTCG+GGG | 0.635268 | 5.1:+65355508 | None:intergenic |
| ATCACCTCCAATGAAGTCAA+GGG | 0.636895 | 5.1:+65355368 | None:intergenic |
| TTCCGGTTATGAACACGTCG+TGG | 0.644049 | 5.1:+65361252 | None:intergenic |
| GATGCTTCTTTGGCTTATGG+CGG | 0.646782 | 5.1:-65360013 | MS.gene071230:CDS |
| TAGGTCCTACTGAACCGCGA+AGG | 0.662127 | 5.1:-65355419 | MS.gene071230:CDS |
| AAGACCACCAAAGTGAGTTG+TGG | 0.662641 | 5.1:+65359652 | None:intergenic |
| CTTGTTCGGAGGCTAATCGA+CGG | 0.665781 | 5.1:+65361288 | None:intergenic |
| TGGCATCAAGGTCTATAAGG+AGG | 0.668182 | 5.1:-65359772 | MS.gene071230:CDS |
| GTTAACAATCCTTTCAGACG+AGG | 0.669845 | 5.1:+65359953 | None:intergenic |
| TCGAATTGGAACTTGTTCGG+AGG | 0.677063 | 5.1:+65361277 | None:intergenic |
| AACTGAAGCTAACCTCTAGG+CGG | 0.686654 | 5.1:+65355139 | None:intergenic |
| AGCGACAGAGGCGAACTTTG+GGG | 0.692963 | 5.1:-65359984 | MS.gene071230:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 65354481 | 65361486 | 65354481 | ID=MS.gene071230 |
| chr5.1 | mRNA | 65354481 | 65361486 | 65354481 | ID=MS.gene071230.t1;Parent=MS.gene071230 |
| chr5.1 | exon | 65361001 | 65361486 | 65361001 | ID=MS.gene071230.t1.exon1;Parent=MS.gene071230.t1 |
| chr5.1 | CDS | 65361001 | 65361486 | 65361001 | ID=cds.MS.gene071230.t1;Parent=MS.gene071230.t1 |
| chr5.1 | exon | 65359616 | 65360101 | 65359616 | ID=MS.gene071230.t1.exon2;Parent=MS.gene071230.t1 |
| chr5.1 | CDS | 65359616 | 65360101 | 65359616 | ID=cds.MS.gene071230.t1;Parent=MS.gene071230.t1 |
| chr5.1 | exon | 65355152 | 65355649 | 65355152 | ID=MS.gene071230.t1.exon3;Parent=MS.gene071230.t1 |
| chr5.1 | CDS | 65355152 | 65355649 | 65355152 | ID=cds.MS.gene071230.t1;Parent=MS.gene071230.t1 |
| chr5.1 | exon | 65354481 | 65354570 | 65354481 | ID=MS.gene071230.t1.exon4;Parent=MS.gene071230.t1 |
| chr5.1 | CDS | 65354481 | 65354570 | 65354481 | ID=cds.MS.gene071230.t1;Parent=MS.gene071230.t1 |
| Gene Sequence |
| Protein sequence |
