AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene056580


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene056580.t1 MTR_3g093380 81.818 33 6 0 1 33 10 42 6.41e-13 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 32 sgRNAs with CRISPR-Local

Find 85 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGCTACAAACACTTCGAAAA+TGG 0.187207 8.3:+30291500 MS.gene056580:CDS
TGCAGCATGGCGGCAGGTTT+TGG 0.247997 8.3:+30292356 MS.gene056580:intron
TGAATTTGAATTCGCTCAAC+TGG 0.286417 8.3:-30291599 None:intergenic
TCGAAAATGGTTTCAAGCTT+TGG 0.295930 8.3:+30291513 MS.gene056580:CDS
GGGGTACTACAAGATAATTT+TGG 0.361728 8.3:-30292511 None:intergenic
AGCCTTAGTCGACATAGATT+TGG 0.372245 8.3:-30292568 None:intergenic
CGAGTTTGAATTTGTTCGAT+TGG 0.387789 8.3:-30291479 None:intergenic
AAATGGTTTCAAGCTTTGGC+TGG 0.447573 8.3:+30291517 MS.gene056580:CDS
GAGTTTGAATTTGTTCGATT+GGG 0.450387 8.3:-30291478 None:intergenic
ATGGGTGGGCCAGTCTCAAT+TGG 0.455108 8.3:+30292410 MS.gene056580:CDS
CATTGTTATTTGGTATGGGT+GGG 0.461412 8.3:+30292396 MS.gene056580:CDS
CAATTTGCAATGGCTCAAGC+TGG 0.462608 8.3:+30292437 MS.gene056580:CDS
TCATTGTTATTTGGTATGGG+TGG 0.474154 8.3:+30292395 MS.gene056580:CDS
ATTTGTTGCAGCATGGCGGC+AGG 0.492718 8.3:+30292350 MS.gene056580:intron
TCTACTGCTCTAGGACTCCA+TGG 0.500255 8.3:-30292533 None:intergenic
GGCTCAAGCTGGGAATTTCA+AGG 0.501155 8.3:+30292448 MS.gene056580:CDS
CTACTGCTCTAGGACTCCAT+GGG 0.502541 8.3:-30292532 None:intergenic
TGCCAAATCTATGTCGACTA+AGG 0.503501 8.3:+30292566 MS.gene056580:CDS
AATTGTATTCCAATTGAGAC+TGG 0.521258 8.3:-30292419 None:intergenic
TTCAAGCTTTGGCTGGAACA+AGG 0.532541 8.3:+30291524 MS.gene056580:CDS
AGAGTACGCTCTACTGCTCT+AGG 0.556845 8.3:-30292542 None:intergenic
AATTGGAATACAATTTGCAA+TGG 0.570489 8.3:+30292427 MS.gene056580:CDS
GAATTTGAATTCGCTCAACT+GGG 0.574178 8.3:-30291598 None:intergenic
GGGTCTCGAATTTCTTCTTG+CGG 0.580872 8.3:-30291578 None:intergenic
AATTTGCAATGGCTCAAGCT+GGG 0.587849 8.3:+30292438 MS.gene056580:CDS
ACCAAATAACAATGAAAACA+TGG 0.590918 8.3:-30292387 None:intergenic
CAAGGAGCAACGATGTTGAA+AGG 0.599259 8.3:+30291434 None:intergenic
ACAAGGAAAGCAAGGAGCAA+AGG 0.605131 8.3:+30291541 MS.gene056580:CDS
TGGCTGGAACAAGGAAAGCA+AGG 0.617589 8.3:+30291533 MS.gene056580:CDS
TACTGCTCTAGGACTCCATG+GGG 0.648024 8.3:-30292531 None:intergenic
AATTATTTGTTGCAGCATGG+CGG 0.698441 8.3:+30292346 MS.gene056580:intron
ACTGCTCTAGGACTCCATGG+GGG 0.751352 8.3:-30292530 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAAAAAAAAGAAGTTT+AGG - chr8.3:30292034-30292053 None:intergenic 10.0%
!! TTGTTGAATTTGAAAATTTA+GGG + chr8.3:30291856-30291875 MS.gene056580:intron 15.0%
!! TTTGTTGAATTTGAAAATTT+AGG + chr8.3:30291855-30291874 MS.gene056580:intron 15.0%
!!! TTTATTGTTTATGATTTTAC+AGG + chr8.3:30291879-30291898 MS.gene056580:intron 15.0%
!! GATAATCAAATCAAAATACT+GGG - chr8.3:30292286-30292305 None:intergenic 20.0%
!! TACATCATATATGTTTGTTT+AGG - chr8.3:30292238-30292257 None:intergenic 20.0%
!! TGATAATCAAATCAAAATAC+TGG - chr8.3:30292287-30292306 None:intergenic 20.0%
!!! TTTTCATTGTTATTTGGTAT+GGG + chr8.3:30292392-30292411 MS.gene056580:CDS 20.0%
! AATTGGAATACAATTTGCAA+TGG + chr8.3:30292427-30292446 MS.gene056580:CDS 25.0%
! ACCAAATAACAATGAAAACA+TGG - chr8.3:30292390-30292409 None:intergenic 25.0%
! GACCAATAACATACATAAAA+TGG - chr8.3:30292193-30292212 None:intergenic 25.0%
! TAGAAAATGGTTTCAATCTT+TGG + chr8.3:30291645-30291664 MS.gene056580:intron 25.0%
!! ATGGTATATTTTCATGCTTT+AGG + chr8.3:30292154-30292173 MS.gene056580:intron 25.0%
!! CACCATTTTATGTATGTTAT+TGG + chr8.3:30292188-30292207 MS.gene056580:intron 25.0%
!!! AAATATACCATCTTTTGAGA+TGG - chr8.3:30292145-30292164 None:intergenic 25.0%
!!! AATATACCATCTTTTGAGAT+GGG - chr8.3:30292144-30292163 None:intergenic 25.0%
!!! GTATTCGTTGTGTTTTTAAA+AGG + chr8.3:30291965-30291984 MS.gene056580:intron 25.0%
!!! GTTTTCATTGTTATTTGGTA+TGG + chr8.3:30292391-30292410 MS.gene056580:CDS 25.0%
AATTGTATTCCAATTGAGAC+TGG - chr8.3:30292422-30292441 None:intergenic 30.0%
! ATGTTATTGGTCTGTATGAT+TGG + chr8.3:30292201-30292220 MS.gene056580:intron 30.0%
! GAGTTTGAATTTGTTCGATT+GGG - chr8.3:30291481-30291500 None:intergenic 30.0%
! TACAATTATTTGTTGCAGCA+TGG + chr8.3:30292343-30292362 MS.gene056580:intron 30.0%
!! GCCATGTTTTCATTGTTATT+TGG + chr8.3:30292386-30292405 MS.gene056580:CDS 30.0%
!! TGTGTTTTTAAAAGGTTGAG+AGG + chr8.3:30291973-30291992 MS.gene056580:intron 30.0%
!! TTTTAAAAGGTTGAGAGGAA+AGG + chr8.3:30291978-30291997 MS.gene056580:intron 30.0%
CGCTACAAACAGTTAGAAAA+TGG + chr8.3:30291632-30291651 MS.gene056580:intron 35.0%
GAAAGGAAGATGTTCTATCT+AGG + chr8.3:30291995-30292014 MS.gene056580:intron 35.0%
GAATTTGAATTCGCTCAACT+GGG - chr8.3:30291601-30291620 None:intergenic 35.0%
TCGAAAATGGTTTCAAGCTT+TGG + chr8.3:30291513-30291532 MS.gene056580:CDS 35.0%
TGAATTTGAATTCGCTCAAC+TGG - chr8.3:30291602-30291621 None:intergenic 35.0%
! AATTATTTGTTGCAGCATGG+CGG + chr8.3:30292346-30292365 MS.gene056580:intron 35.0%
! CGAGTTTGAATTTGTTCGAT+TGG - chr8.3:30291482-30291501 None:intergenic 35.0%
! TTTTGTATTCACTATGCCGT+GGG + chr8.3:30291757-30291776 MS.gene056580:intron 35.0%
!! CATTGTTATTTGGTATGGGT+GGG + chr8.3:30292396-30292415 MS.gene056580:CDS 35.0%
!! GGGGTACTACAAGATAATTT+TGG - chr8.3:30292514-30292533 None:intergenic 35.0%
!! TCATTGTTATTTGGTATGGG+TGG + chr8.3:30292395-30292414 MS.gene056580:CDS 35.0%
!!! ATTGGGTTTTGAAGTTGTTG+TGG - chr8.3:30291464-30291483 None:intergenic 35.0%
AATTTGCAATGGCTCAAGCT+GGG + chr8.3:30292438-30292457 MS.gene056580:CDS 40.0%
AGATTCTGAGCAGGAATCAA+AGG - chr8.3:30291919-30291938 None:intergenic 40.0%
ATTATCTTGTAGTACCCCCA+TGG + chr8.3:30292516-30292535 MS.gene056580:CDS 40.0%
CACGGCATAGTGAATACAAA+AGG - chr8.3:30291758-30291777 None:intergenic 40.0%
CGCTACAAACACTTCGAAAA+TGG + chr8.3:30291500-30291519 MS.gene056580:CDS 40.0%
! AAATGGTTTCAAGCTTTGGC+TGG + chr8.3:30291517-30291536 MS.gene056580:CDS 40.0%
! CTTTTGTATTCACTATGCCG+TGG + chr8.3:30291756-30291775 MS.gene056580:intron 40.0%
!!! ATACCATCTTTTGAGATGGG+TGG - chr8.3:30292141-30292160 None:intergenic 40.0%
AAACATGACTCCTGCTCATC+AGG + chr8.3:30291802-30291821 MS.gene056580:intron 45.0%
ACAACAGCAAGATTCTGAGC+AGG - chr8.3:30291928-30291947 None:intergenic 45.0%
ACAAGGAAAGCAAGGAGCAA+AGG + chr8.3:30291541-30291560 MS.gene056580:CDS 45.0%
ACCAACAGCGAACCATTTAG+CGG - chr8.3:30291723-30291742 None:intergenic 45.0%
CAATTTGCAATGGCTCAAGC+TGG + chr8.3:30292437-30292456 MS.gene056580:CDS 45.0%
GGGTCTCGAATTTCTTCTTG+CGG - chr8.3:30291581-30291600 None:intergenic 45.0%
! TTCAAGCTTTGGCTGGAACA+AGG + chr8.3:30291524-30291543 MS.gene056580:CDS 45.0%
!! TGCTCAGAATCTTGCTGTTG+TGG + chr8.3:30291927-30291946 MS.gene056580:intron 45.0%
AACAGCGAACCATTTAGCGG+CGG - chr8.3:30291720-30291739 None:intergenic 50.0%
AGAGTACGCTCTACTGCTCT+AGG - chr8.3:30292545-30292564 None:intergenic 50.0%
CTGCCACCCATCTCAAAAGA+TGG + chr8.3:30292135-30292154 MS.gene056580:intron 50.0%
GGCTCAAGCTGGGAATTTCA+AGG + chr8.3:30292448-30292467 MS.gene056580:CDS 50.0%
TGGCTGGAACAAGGAAAGCA+AGG + chr8.3:30291533-30291552 MS.gene056580:CDS 50.0%
!! CAGAATCTTGCTGTTGTGGC+TGG + chr8.3:30291931-30291950 MS.gene056580:intron 50.0%
!! CTACTGCTCTAGGACTCCAT+GGG - chr8.3:30292535-30292554 None:intergenic 50.0%
!! TACTGCTCTAGGACTCCATG+GGG - chr8.3:30292534-30292553 None:intergenic 50.0%
!! TCTACTGCTCTAGGACTCCA+TGG - chr8.3:30292536-30292555 None:intergenic 50.0%
ATGGGTGGGCCAGTCTCAAT+TGG + chr8.3:30292410-30292429 MS.gene056580:CDS 55.0%
CACTATGCCGTGGGATGTGT+CGG + chr8.3:30291766-30291785 MS.gene056580:intron 55.0%
GAAACCCTGACCTGATGAGC+AGG - chr8.3:30291815-30291834 None:intergenic 55.0%
GACACATCCGACACATCCCA+CGG - chr8.3:30291776-30291795 None:intergenic 55.0%
GACTCCTGCTCATCAGGTCA+GGG + chr8.3:30291808-30291827 MS.gene056580:intron 55.0%
GCCGCTAAATGGTTCGCTGT+TGG + chr8.3:30291719-30291738 MS.gene056580:intron 55.0%
GTTGTGGCTGGAGTGAATGC+TGG + chr8.3:30291943-30291962 MS.gene056580:intron 55.0%
TGACTCCTGCTCATCAGGTC+AGG + chr8.3:30291807-30291826 MS.gene056580:intron 55.0%
! ATTTGTTGCAGCATGGCGGC+AGG + chr8.3:30292350-30292369 MS.gene056580:intron 55.0%
!! ACTGCTCTAGGACTCCATGG+GGG - chr8.3:30292533-30292552 None:intergenic 55.0%
!!! ATGGCGGCAGGTTTTGGTTC+TGG + chr8.3:30292362-30292381 MS.gene056580:CDS 55.0%
!!! TGGCGGCAGGTTTTGGTTCT+GGG + chr8.3:30292363-30292382 MS.gene056580:CDS 55.0%
AGCGAACCATTTAGCGGCGG+TGG - chr8.3:30291717-30291736 None:intergenic 60.0%
GAACCATTTAGCGGCGGTGG+TGG - chr8.3:30291714-30291733 None:intergenic 60.0%
! TGCAGCATGGCGGCAGGTTT+TGG + chr8.3:30292356-30292375 MS.gene056580:intron 60.0%
!!! GGCGGCAGGTTTTGGTTCTG+GGG + chr8.3:30292364-30292383 MS.gene056580:CDS 60.0%
! CCATTTAGCGGCGGTGGTGG+CGG - chr8.3:30291711-30291730 None:intergenic 65.0%
CCGCCACCACCGCCGCTAAA+TGG + chr8.3:30291708-30291727 MS.gene056580:intron 70.0%
! GGTGACGGCGGCGGCTTCAA+CGG - chr8.3:30291690-30291709 None:intergenic 70.0%
GGCGGCGGCTTCAACGGCGA+TGG - chr8.3:30291684-30291703 None:intergenic 75.0%
!! AGCGGCGGTGGTGGCGGTGA+CGG - chr8.3:30291705-30291724 None:intergenic 75.0%
!!! GGCGGTGGTGGCGGTGACGG+CGG - chr8.3:30291702-30291721 None:intergenic 80.0%
!!! GGTGGTGGCGGTGACGGCGG+CGG - chr8.3:30291699-30291718 None:intergenic 80.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 30291446 30292586 30291446 ID=MS.gene056580
chr8.3 mRNA 30291446 30292586 30291446 ID=MS.gene056580.t1;Parent=MS.gene056580
chr8.3 exon 30291446 30291642 30291446 ID=MS.gene056580.t1.exon1;Parent=MS.gene056580.t1
chr8.3 CDS 30291446 30291642 30291446 ID=cds.MS.gene056580.t1;Parent=MS.gene056580.t1
chr8.3 exon 30292361 30292586 30292361 ID=MS.gene056580.t1.exon2;Parent=MS.gene056580.t1
chr8.3 CDS 30292361 30292586 30292361 ID=cds.MS.gene056580.t1;Parent=MS.gene056580.t1
Gene Sequence

>MS.gene056580

ATGTTGAAAGGTTCACCACAACAACTTCAAAACCCAATCGAACAAATTCAAACTCGCTACAAACACTTCGAAAATGGTTTCAAGCTTTGGCTGGAACAAGGAAAGCAAGGAGCAAAGGTGTTGAATGCTTCACCGCAAGAAGAAATTCGAGACCCAGTTGAGCGAATTCAAATTCAATTTCAAACTCGCTACAAACAGTTAGAAAATGGTTTCAATCTTTGGCTCTCTAAACAGTCCATCGCCGTTGAAGCCGCCGCCGTCACCGCCACCACCGCCGCTAAATGGTTCGCTGTTGGTGCTCTTGCTACTCCTTTTGTATTCACTATGCCGTGGGATGTGTCGGATGTGTCTTTTAAAAACATGACTCCTGCTCATCAGGTCAGGGTTTCATTGAATTTTCTAATTTAATTTTGTTGAATTTGAAAATTTAGGGTTTATTGTTTATGATTTTACAGGACTTTATTCGACGTCCTTTGATTCCTGCTCAGAATCTTGCTGTTGTGGCTGGAGTGAATGCTGGTATTCGTTGTGTTTTTAAAAGGTTGAGAGGAAAGGAAGATGTTCTATCTAGGTCAAATTTCTTAACCTAAACTTCTTTTTTTTTTTTTGCAAAGAGTTTATGAAAAAATTAAGTAATAAGCATTAAGATATTGAGTTTTTTCAACATGAATTACATCAAATTTGTTAATCTGCCACCCATCTCAAAAGATGGTATATTTTCATGCTTTAGGCTTTAGTTAATCACCATTTTATGTATGTTATTGGTCTGTATGATTGGTTGTATTGTAGCCTAAACAAACATATATGATGTACTTGAAGAGCTTTCTTAATGCTTTACCCAGTATTTTGATTTGATTATCATTGATGCGTCTTCTTATAGCAGTAATTTAACAGCACTACAATTATTTGTTGCAGCATGGCGGCAGGTTTTGGTTCTGGGGCCATGTTTTCATTGTTATTTGGTATGGGTGGGCCAGTCTCAATTGGAATACAATTTGCAATGGCTCAAGCTGGGAATTTCAAGGTGAGCATGAACAAAATGCATCTTTTTGACAGCTTTGTAAACCAAAATTATCTTGTAGTACCCCCATGGAGTCCTAGAGCAGTAGAGCGTACTCTATGCCAAATCTATGTCGACTAA

Protein sequence

>MS.gene056580.t1

MLKGSPQQLQNPIEQIQTRYKHFENGFKLWLEQGKQGAKVLNASPQEEIRDPVERIQIQFQTRYKHMAAGFGSGAMFSLLFGMGGPVSIGIQFAMAQAGNFKVSMNKMHLFDSFVNQNYLVVPPWSPRAVERTLCQIYVD