Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071330.t1 | GAU44816.1 | 39.6 | 230 | 22 | 3 | 23 | 139 | 477 | 702 | 3.00E-26 | 128.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071330.t1 | A0A2Z6NMD3 | 39.6 | 230 | 22 | 3 | 23 | 139 | 477 | 702 | 2.2e-26 | 128.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049262 | MS.gene071330 | 0.800044 | 1.73E-48 | -1.69E-46 |
| MS.gene049853 | MS.gene071330 | 0.832835 | 7.54E-56 | -1.69E-46 |
| MS.gene05404 | MS.gene071330 | 0.808031 | 3.78E-50 | -1.69E-46 |
| MS.gene054484 | MS.gene071330 | 0.831838 | 1.33E-55 | -1.69E-46 |
| MS.gene055017 | MS.gene071330 | 0.810639 | 1.04E-50 | -1.69E-46 |
| MS.gene055056 | MS.gene071330 | 0.822038 | 2.93E-53 | -1.69E-46 |
| MS.gene055058 | MS.gene071330 | 0.815918 | 7.23E-52 | -1.69E-46 |
| MS.gene058515 | MS.gene071330 | 0.833074 | 6.57E-56 | -1.69E-46 |
| MS.gene05925 | MS.gene071330 | 0.802488 | 5.48E-49 | -1.69E-46 |
| MS.gene059276 | MS.gene071330 | 0.838975 | 2.09E-57 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071330.t1 | MTR_0152s0010 | 59.375 | 64 | 25 | 1 | 14 | 76 | 10 | 73 | 8.11e-20 | 79.3 |
| MS.gene071330.t1 | MTR_0152s0060 | 57.812 | 64 | 26 | 1 | 14 | 76 | 45 | 108 | 3.07e-18 | 80.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 31 sgRNAs with CRISPR-Local
Find 101 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CACACATATTGTTGACTTGA+AGG | 0.331521 | 7.3:-34329653 | None:intergenic |
| GTTACCTTATTTCCACCTTT+AGG | 0.355704 | 7.3:-34330406 | None:intergenic |
| GATGCCTAAAGGTGGAAATA+AGG | 0.378831 | 7.3:+34330402 | MS.gene071330:CDS |
| TTTGTCACGAATATCCAATA+TGG | 0.387906 | 7.3:-34329691 | None:intergenic |
| AAGGCGAAGGTGGTGGAGAA+AGG | 0.417691 | 7.3:+34329106 | MS.gene071330:CDS |
| AGCATTCAACAAAACCATAT+TGG | 0.419572 | 7.3:+34329677 | MS.gene071330:CDS |
| CGTGATGAATTCATCTATGA+AGG | 0.429871 | 7.3:+34329087 | None:intergenic |
| CCCTCAAGCAATGTGATGAT+AGG | 0.452056 | 7.3:-34329714 | None:intergenic |
| GAAAGTTTGGTCACAATATG+AGG | 0.455338 | 7.3:+34330347 | MS.gene071330:CDS |
| CCTATCATCACATTGCTTGA+GGG | 0.464361 | 7.3:+34329714 | MS.gene071330:CDS |
| AGGTGGTGGAGAAAGGAGAA+GGG | 0.490083 | 7.3:+34329113 | MS.gene071330:CDS |
| AAGGTGGTGGAGAAAGGAGA+AGG | 0.492351 | 7.3:+34329112 | MS.gene071330:CDS |
| ATCTATGAAGGCGAAGGTGG+TGG | 0.496255 | 7.3:+34329099 | MS.gene071330:CDS |
| GATTGTCACTCAAAGTGAAC+TGG | 0.501960 | 7.3:+34329761 | MS.gene071330:CDS |
| GAGATGCATCTCTGTATTCA+AGG | 0.510095 | 7.3:+34329140 | MS.gene071330:CDS |
| ACCTATCATCACATTGCTTG+AGG | 0.523184 | 7.3:+34329713 | MS.gene071330:CDS |
| GCTTGTGATCCTCGAGGTTG+AGG | 0.527688 | 7.3:-34330579 | None:intergenic |
| GCAGATAGAGCGATGCCTAA+AGG | 0.555906 | 7.3:+34330391 | MS.gene071330:CDS |
| GTGGTGGAGAAAGGAGAAGG+GGG | 0.563580 | 7.3:+34329115 | MS.gene071330:CDS |
| TTCATCTATGAAGGCGAAGG+TGG | 0.570196 | 7.3:+34329096 | MS.gene071330:CDS |
| CACAATATGAGGACTTGCAA+GGG | 0.581035 | 7.3:+34330358 | MS.gene071330:CDS |
| TAAGTCACAAAAGAAGTCAC+AGG | 0.591437 | 7.3:+34330536 | MS.gene071330:CDS |
| TCACAATATGAGGACTTGCA+AGG | 0.596985 | 7.3:+34330357 | MS.gene071330:CDS |
| GAATTCATCTATGAAGGCGA+AGG | 0.623904 | 7.3:+34329093 | MS.gene071330:CDS |
| ATCCATCTAGTAAATGGACA+AGG | 0.650961 | 7.3:+34329601 | MS.gene071330:intron |
| GGTGGTGGAGAAAGGAGAAG+GGG | 0.655309 | 7.3:+34329114 | MS.gene071330:CDS |
| GATAGAGCGATGCCTAAAGG+TGG | 0.657666 | 7.3:+34330394 | MS.gene071330:CDS |
| TCACAAAAGAAGTCACAGGA+TGG | 0.672212 | 7.3:+34330540 | MS.gene071330:CDS |
| CTTGATGCTTGTGATCCTCG+AGG | 0.696899 | 7.3:-34330585 | None:intergenic |
| GACTTGCAAGGGTAAAACAG+CGG | 0.708631 | 7.3:+34330369 | MS.gene071330:CDS |
| TCACAAGCACCTCAACCTCG+AGG | 0.713929 | 7.3:+34330570 | MS.gene071330:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TGAAAAATTATATTTCAAAA+AGG | + | chr7.3:34329739-34329758 | MS.gene071330:CDS | 10.0% |
| !! | TTAATTTCATCAATTCATAT+AGG | + | chr7.3:34330114-34330133 | MS.gene071330:intron | 15.0% |
| ! | CAAATGAGACTATAATGTTA+TGG | - | chr7.3:34329381-34329400 | None:intergenic | 25.0% |
| ! | CATAACATTATAGTCTCATT+TGG | + | chr7.3:34329379-34329398 | MS.gene071330:intron | 25.0% |
| ! | CTGTAATAAATAGTGTATCA+CGG | + | chr7.3:34329316-34329335 | MS.gene071330:intron | 25.0% |
| ! | TGATACATATTCTAACTGTA+CGG | + | chr7.3:34330429-34330448 | MS.gene071330:intron | 25.0% |
| !! | AATTTTGTATCTCTAGTTGA+TGG | + | chr7.3:34329514-34329533 | MS.gene071330:intron | 25.0% |
| !! | ATTGACTCACTATCTATAAT+AGG | - | chr7.3:34330203-34330222 | None:intergenic | 25.0% |
| !! | GATATGTTGATAAGTCTATT+TGG | + | chr7.3:34329562-34329581 | MS.gene071330:intron | 25.0% |
| !!! | ACTCTTGTTTCTTTGTTTTT+TGG | - | chr7.3:34330261-34330280 | None:intergenic | 25.0% |
| !!! | CTCTTGTTTCTTTGTTTTTT+GGG | - | chr7.3:34330260-34330279 | None:intergenic | 25.0% |
| AACTGTGAATGCAAAAATTG+TGG | + | chr7.3:34330325-34330344 | MS.gene071330:CDS | 30.0% | |
| AGAATCCTAAAATACTTCCA+AGG | + | chr7.3:34330294-34330313 | MS.gene071330:intron | 30.0% | |
| AGCATTCAACAAAACCATAT+TGG | + | chr7.3:34329677-34329696 | MS.gene071330:CDS | 30.0% | |
| GAATCCTAAAATACTTCCAA+GGG | + | chr7.3:34330295-34330314 | MS.gene071330:intron | 30.0% | |
| TCACGTAATGAAAAGACATT+AGG | - | chr7.3:34329188-34329207 | None:intergenic | 30.0% | |
| TGAAACATCCATCTAGTAAA+TGG | + | chr7.3:34329595-34329614 | MS.gene071330:intron | 30.0% | |
| TGCAAAAATTGTGGAAAGTT+TGG | + | chr7.3:34330334-34330353 | MS.gene071330:CDS | 30.0% | |
| TTTGTCACGAATATCCAATA+TGG | - | chr7.3:34329694-34329713 | None:intergenic | 30.0% | |
| ! | ACAATTTTTGCATTCACAGT+TGG | - | chr7.3:34330326-34330345 | None:intergenic | 30.0% |
| ! | ATTCTGTTGCTTCATATTAC+AGG | + | chr7.3:34330039-34330058 | MS.gene071330:intron | 30.0% |
| !!! | GGAAGTATTTTAGGATTCTT+GGG | - | chr7.3:34330293-34330312 | None:intergenic | 30.0% |
| !!! | TGGAAGTATTTTAGGATTCT+TGG | - | chr7.3:34330294-34330313 | None:intergenic | 30.0% |
| AATAGTGTATCACGGAGTAA+TGG | + | chr7.3:34329324-34329343 | MS.gene071330:intron | 35.0% | |
| ATCCATCTAGTAAATGGACA+AGG | + | chr7.3:34329601-34329620 | MS.gene071330:intron | 35.0% | |
| CTCTAGTATTGCTGAAGTTA+AGG | + | chr7.3:34329426-34329445 | MS.gene071330:intron | 35.0% | |
| GAAAGTTTGGTCACAATATG+AGG | + | chr7.3:34330347-34330366 | MS.gene071330:CDS | 35.0% | |
| TAAGTCACAAAAGAAGTCAC+AGG | + | chr7.3:34330536-34330555 | MS.gene071330:CDS | 35.0% | |
| TCCCATTCTCTACATGAAAA+AGG | - | chr7.3:34329899-34329918 | None:intergenic | 35.0% | |
| TCGCTGATCATGAATATTAG+TGG | + | chr7.3:34329241-34329260 | MS.gene071330:intron | 35.0% | |
| TCTATAATAGGCCAAAGCTT+TGG | - | chr7.3:34330191-34330210 | None:intergenic | 35.0% | |
| ! | GTTACCTTATTTCCACCTTT+AGG | - | chr7.3:34330409-34330428 | None:intergenic | 35.0% |
| ! | TTTTTGCATTCACAGTTGGA+AGG | - | chr7.3:34330322-34330341 | None:intergenic | 35.0% |
| !! | ATGCTGTCGATTTACACAAA+AGG | + | chr7.3:34329928-34329947 | MS.gene071330:intron | 35.0% |
| !! | CACACATATTGTTGACTTGA+AGG | - | chr7.3:34329656-34329675 | None:intergenic | 35.0% |
| !! | GCCTTTTTCATGTAGAGAAT+GGG | + | chr7.3:34329895-34329914 | MS.gene071330:intron | 35.0% |
| !! | TTAGTGGTTTGTTCAAACTG+TGG | + | chr7.3:34329257-34329276 | MS.gene071330:intron | 35.0% |
| ACAAAAGCCAAAGACTACCT+AGG | + | chr7.3:34329214-34329233 | MS.gene071330:intron | 40.0% | |
| ACCTATCATCACATTGCTTG+AGG | + | chr7.3:34329713-34329732 | MS.gene071330:CDS | 40.0% | |
| CACAATATGAGGACTTGCAA+GGG | + | chr7.3:34330358-34330377 | MS.gene071330:CDS | 40.0% | |
| CACATCATCATGCCTTCAAA+TGG | + | chr7.3:34330164-34330183 | MS.gene071330:intron | 40.0% | |
| CCTATCATCACATTGCTTGA+GGG | + | chr7.3:34329714-34329733 | MS.gene071330:CDS | 40.0% | |
| CGATTAACCTAGGTAGTCTT+TGG | - | chr7.3:34329224-34329243 | None:intergenic | 40.0% | |
| CTCCTTGTCCATTTACTAGA+TGG | - | chr7.3:34329606-34329625 | None:intergenic | 40.0% | |
| CTTCAAATGGTCCAAAGCTT+TGG | + | chr7.3:34330177-34330196 | MS.gene071330:intron | 40.0% | |
| GAATTCATCTATGAAGGCGA+AGG | + | chr7.3:34329093-34329112 | MS.gene071330:CDS | 40.0% | |
| GAGATGCATCTCTGTATTCA+AGG | + | chr7.3:34329140-34329159 | MS.gene071330:CDS | 40.0% | |
| GATCCAACTATTGCTCGAAA+AGG | + | chr7.3:34329815-34329834 | MS.gene071330:intron | 40.0% | |
| GATGCCTAAAGGTGGAAATA+AGG | + | chr7.3:34330402-34330421 | MS.gene071330:CDS | 40.0% | |
| GATTGTCACTCAAAGTGAAC+TGG | + | chr7.3:34329761-34329780 | MS.gene071330:CDS | 40.0% | |
| TATGAAGCAACAGAATCCTC+TGG | - | chr7.3:34330035-34330054 | None:intergenic | 40.0% | |
| TCACAAAAGAAGTCACAGGA+TGG | + | chr7.3:34330540-34330559 | MS.gene071330:CDS | 40.0% | |
| TCACAATATGAGGACTTGCA+AGG | + | chr7.3:34330357-34330376 | MS.gene071330:CDS | 40.0% | |
| TCATGATCAGCGATTAACCT+AGG | - | chr7.3:34329234-34329253 | None:intergenic | 40.0% | |
| TCGAGCAATAGTTGGATCTT+TGG | - | chr7.3:34329813-34329832 | None:intergenic | 40.0% | |
| TGCAGAAAATGGGATTTGAC+TGG | + | chr7.3:34329960-34329979 | MS.gene071330:intron | 40.0% | |
| !! | CAAAGCTTTGGACCATTTGA+AGG | - | chr7.3:34330179-34330198 | None:intergenic | 40.0% |
| !! | GGCCTTTTTCATGTAGAGAA+TGG | + | chr7.3:34329894-34329913 | MS.gene071330:intron | 40.0% |
| !!! | AGGTCCCTTGGAAGTATTTT+AGG | - | chr7.3:34330302-34330321 | None:intergenic | 40.0% |
| ATTGCTCGAAAAGGCCAAGA+GGG | + | chr7.3:34329824-34329843 | MS.gene071330:intron | 45.0% | |
| CCCTCAAGCAATGTGATGAT+AGG | - | chr7.3:34329717-34329736 | None:intergenic | 45.0% | |
| GACTTGCAAGGGTAAAACAG+CGG | + | chr7.3:34330369-34330388 | MS.gene071330:CDS | 45.0% | |
| GACTTGCGCTTGCAGAAAAT+GGG | + | chr7.3:34329950-34329969 | MS.gene071330:intron | 45.0% | |
| GCATAACCATGAAGTTCCAG+AGG | + | chr7.3:34330016-34330035 | MS.gene071330:intron | 45.0% | |
| TATTGCTCGAAAAGGCCAAG+AGG | + | chr7.3:34329823-34329842 | MS.gene071330:intron | 45.0% | |
| TTCATCTATGAAGGCGAAGG+TGG | + | chr7.3:34329096-34329115 | MS.gene071330:CDS | 45.0% | |
| TTCATGGTTATGCCACATGC+AGG | - | chr7.3:34330009-34330028 | None:intergenic | 45.0% | |
| ! | TGAACTGGTGCGAAGGTATA+CGG | + | chr7.3:34329776-34329795 | MS.gene071330:intron | 45.0% |
| !! | CAGAATCCTCTGGAACTTCA+TGG | - | chr7.3:34330025-34330044 | None:intergenic | 45.0% |
| !! | GTTATGGTGCTACAGCGTAT+CGG | - | chr7.3:34329365-34329384 | None:intergenic | 45.0% |
| !! | TGGCCTTTTCGAGCAATAGT+TGG | - | chr7.3:34329821-34329840 | None:intergenic | 45.0% |
| AGATGGGTGGTCTCCACATT+GGG | + | chr7.3:34329851-34329870 | MS.gene071330:intron | 50.0% | |
| ATCTATGAAGGCGAAGGTGG+TGG | + | chr7.3:34329099-34329118 | MS.gene071330:CDS | 50.0% | |
| CAGGCAATGCTATGGCAACA+CGG | - | chr7.3:34329990-34330009 | None:intergenic | 50.0% | |
| CCTGACGCTCTTCTCATACT+TGG | - | chr7.3:34330233-34330252 | None:intergenic | 50.0% | |
| CTCAAAGTGAACTGGTGCGA+AGG | + | chr7.3:34329769-34329788 | MS.gene071330:intron | 50.0% | |
| CTTGATGCTTGTGATCCTCG+AGG | - | chr7.3:34330588-34330607 | None:intergenic | 50.0% | |
| GACGCTCTTCTCATACTTGG+TGG | - | chr7.3:34330230-34330249 | None:intergenic | 50.0% | |
| GATAGAGCGATGCCTAAAGG+TGG | + | chr7.3:34330394-34330413 | MS.gene071330:CDS | 50.0% | |
| GCAGATAGAGCGATGCCTAA+AGG | + | chr7.3:34330391-34330410 | MS.gene071330:CDS | 50.0% | |
| GGACTTGCGCTTGCAGAAAA+TGG | + | chr7.3:34329949-34329968 | MS.gene071330:intron | 50.0% | |
| TCTAGATCACCAGCCCAATG+TGG | - | chr7.3:34329867-34329886 | None:intergenic | 50.0% | |
| TTCACAGTTGGAAGGTCCCT+TGG | - | chr7.3:34330314-34330333 | None:intergenic | 50.0% | |
| ! | GAACTGGTGCGAAGGTATAC+GGG | + | chr7.3:34329777-34329796 | MS.gene071330:intron | 50.0% |
| ! | GCTGGTGATCTAGAGCATCA+TGG | + | chr7.3:34329873-34329892 | MS.gene071330:intron | 50.0% |
| !! | AAGGTGGTGGAGAAAGGAGA+AGG | + | chr7.3:34329112-34329131 | MS.gene071330:CDS | 50.0% |
| !! | AGGTGGTGGAGAAAGGAGAA+GGG | + | chr7.3:34329113-34329132 | MS.gene071330:CDS | 50.0% |
| !! | CCAAGTATGAGAAGAGCGTC+AGG | + | chr7.3:34330230-34330249 | MS.gene071330:intron | 50.0% |
| CAGATGGGTGGTCTCCACAT+TGG | + | chr7.3:34329850-34329869 | MS.gene071330:intron | 55.0% | |
| GCCACATGCAGGCAATGCTA+TGG | - | chr7.3:34329998-34330017 | None:intergenic | 55.0% | |
| GCCATAGCATTGCCTGCATG+TGG | + | chr7.3:34329994-34330013 | MS.gene071330:intron | 55.0% | |
| GCTTGTGATCCTCGAGGTTG+AGG | - | chr7.3:34330582-34330601 | None:intergenic | 55.0% | |
| TCACAAGCACCTCAACCTCG+AGG | + | chr7.3:34330570-34330589 | MS.gene071330:CDS | 55.0% | |
| ! | GTGGTGGAGAAAGGAGAAGG+GGG | + | chr7.3:34329115-34329134 | MS.gene071330:CDS | 55.0% |
| !! | AAGGCGAAGGTGGTGGAGAA+AGG | + | chr7.3:34329106-34329125 | MS.gene071330:CDS | 55.0% |
| !! | GGTGGTGGAGAAAGGAGAAG+GGG | + | chr7.3:34329114-34329133 | MS.gene071330:CDS | 55.0% |
| AAGGCCAAGAGGGTCGCAGA+TGG | + | chr7.3:34329834-34329853 | MS.gene071330:intron | 60.0% | |
| AGGCCAAGAGGGTCGCAGAT+GGG | + | chr7.3:34329835-34329854 | MS.gene071330:intron | 60.0% | |
| CCAAGAGGGTCGCAGATGGG+TGG | + | chr7.3:34329838-34329857 | MS.gene071330:intron | 65.0% | |
| CCACCCATCTGCGACCCTCT+TGG | - | chr7.3:34329841-34329860 | None:intergenic | 65.0% | |
| GGGTGGTCTCCACATTGGGC+TGG | + | chr7.3:34329855-34329874 | MS.gene071330:intron | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 34329091 | 34330614 | 34329091 | ID=MS.gene071330 |
| chr7.3 | mRNA | 34329091 | 34330614 | 34329091 | ID=MS.gene071330.t1;Parent=MS.gene071330 |
| chr7.3 | exon | 34329091 | 34329161 | 34329091 | ID=MS.gene071330.t1.exon1;Parent=MS.gene071330.t1 |
| chr7.3 | CDS | 34329091 | 34329161 | 34329091 | ID=cds.MS.gene071330.t1;Parent=MS.gene071330.t1 |
| chr7.3 | exon | 34329611 | 34329782 | 34329611 | ID=MS.gene071330.t1.exon2;Parent=MS.gene071330.t1 |
| chr7.3 | CDS | 34329611 | 34329782 | 34329611 | ID=cds.MS.gene071330.t1;Parent=MS.gene071330.t1 |
| chr7.3 | exon | 34330316 | 34330423 | 34330316 | ID=MS.gene071330.t1.exon3;Parent=MS.gene071330.t1 |
| chr7.3 | CDS | 34330316 | 34330423 | 34330316 | ID=cds.MS.gene071330.t1;Parent=MS.gene071330.t1 |
| chr7.3 | exon | 34330519 | 34330614 | 34330519 | ID=MS.gene071330.t1.exon4;Parent=MS.gene071330.t1 |
| chr7.3 | CDS | 34330519 | 34330614 | 34330519 | ID=cds.MS.gene071330.t1;Parent=MS.gene071330.t1 |
| Gene Sequence |
| Protein sequence |
