Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072434.t1 | XP_003626337.1 | 95.4 | 151 | 3 | 1 | 1 | 151 | 1 | 147 | 1.10E-71 | 279.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072434.t1 | Q9ZNV9 | 59.2 | 147 | 59 | 1 | 4 | 150 | 3 | 148 | 1.3e-44 | 180.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072434.t1 | A2Q3H8 | 95.4 | 151 | 3 | 1 | 1 | 151 | 1 | 147 | 7.9e-72 | 279.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050170 | MS.gene072434 | -0.808223 | 3.44E-50 | -1.69E-46 |
| MS.gene050326 | MS.gene072434 | 0.802906 | 4.49E-49 | -1.69E-46 |
| MS.gene052494 | MS.gene072434 | 0.815392 | 9.47E-52 | -1.69E-46 |
| MS.gene054941 | MS.gene072434 | 0.817361 | 3.43E-52 | -1.69E-46 |
| MS.gene056387 | MS.gene072434 | -0.808377 | 3.19E-50 | -1.69E-46 |
| MS.gene05652 | MS.gene072434 | 0.80269 | 4.98E-49 | -1.69E-46 |
| MS.gene056618 | MS.gene072434 | 0.844352 | 7.97E-59 | -1.69E-46 |
| MS.gene057367 | MS.gene072434 | 0.826307 | 2.92E-54 | -1.69E-46 |
| MS.gene058382 | MS.gene072434 | 0.832386 | 9.74E-56 | -1.69E-46 |
| MS.gene05926 | MS.gene072434 | 0.814635 | 1.39E-51 | -1.69E-46 |
| MS.gene05960 | MS.gene072434 | 0.802585 | 5.23E-49 | -1.69E-46 |
| MS.gene06042 | MS.gene072434 | 0.854497 | 1.18E-61 | -1.69E-46 |
| MS.gene06093 | MS.gene072434 | 0.804722 | 1.88E-49 | -1.69E-46 |
| MS.gene061044 | MS.gene072434 | 0.827413 | 1.59E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072434.t1 | MTR_7g114020 | 95.364 | 151 | 3 | 1 | 1 | 151 | 1 | 147 | 6.73e-102 | 288 |
| MS.gene072434.t1 | MTR_1g082290 | 66.207 | 145 | 48 | 1 | 1 | 145 | 1 | 144 | 9.35e-68 | 202 |
| MS.gene072434.t1 | MTR_2g020770 | 61.224 | 147 | 56 | 1 | 4 | 150 | 3 | 148 | 2.94e-57 | 176 |
| MS.gene072434.t1 | MTR_1g089130 | 50.350 | 143 | 70 | 1 | 6 | 148 | 3 | 144 | 5.78e-52 | 162 |
| MS.gene072434.t1 | MTR_2g020770 | 59.124 | 137 | 55 | 1 | 4 | 140 | 3 | 138 | 1.62e-49 | 155 |
| MS.gene072434.t1 | MTR_2g100900 | 44.030 | 134 | 74 | 1 | 8 | 141 | 7 | 139 | 1.14e-38 | 128 |
| MS.gene072434.t1 | MTR_2g100880 | 44.030 | 134 | 74 | 1 | 8 | 141 | 7 | 139 | 1.55e-38 | 128 |
| MS.gene072434.t1 | MTR_2g103870 | 44.776 | 134 | 73 | 1 | 18 | 150 | 20 | 153 | 3.37e-30 | 107 |
| MS.gene072434.t1 | MTR_4g010160 | 46.324 | 136 | 72 | 1 | 16 | 150 | 6 | 141 | 1.48e-27 | 100 |
| MS.gene072434.t1 | MTR_2g086010 | 27.465 | 142 | 101 | 2 | 3 | 143 | 2 | 142 | 3.92e-12 | 60.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072434.t1 | AT1G03430 | 54.730 | 148 | 67 | 0 | 4 | 151 | 6 | 153 | 1.43e-57 | 177 |
| MS.gene072434.t1 | AT5G39340 | 52.703 | 148 | 70 | 0 | 4 | 151 | 5 | 152 | 1.58e-55 | 171 |
| MS.gene072434.t1 | AT5G39340 | 52.703 | 148 | 70 | 0 | 4 | 151 | 38 | 185 | 5.69e-55 | 171 |
| MS.gene072434.t1 | AT3G29350 | 52.027 | 148 | 71 | 0 | 4 | 151 | 5 | 152 | 1.03e-54 | 169 |
| MS.gene072434.t1 | AT5G39340 | 52.703 | 148 | 70 | 0 | 4 | 151 | 54 | 201 | 1.33e-54 | 171 |
| MS.gene072434.t1 | AT5G39340 | 52.703 | 148 | 70 | 0 | 4 | 151 | 54 | 201 | 1.33e-54 | 171 |
| MS.gene072434.t1 | AT3G21510 | 59.184 | 147 | 59 | 1 | 4 | 150 | 3 | 148 | 3.37e-54 | 168 |
| MS.gene072434.t1 | AT1G03430 | 54.676 | 139 | 63 | 0 | 4 | 142 | 6 | 144 | 2.19e-51 | 161 |
| MS.gene072434.t1 | AT3G29350 | 56.364 | 110 | 48 | 0 | 4 | 113 | 5 | 114 | 2.20e-42 | 137 |
| MS.gene072434.t1 | AT3G16360 | 41.481 | 135 | 78 | 1 | 7 | 141 | 1 | 134 | 1.02e-38 | 128 |
| MS.gene072434.t1 | AT3G16360 | 41.667 | 120 | 69 | 1 | 21 | 140 | 9 | 127 | 1.26e-34 | 117 |
| MS.gene072434.t1 | AT1G80100 | 42.282 | 149 | 85 | 1 | 3 | 150 | 5 | 153 | 1.32e-24 | 93.2 |
| MS.gene072434.t1 | AT1G80100 | 41.176 | 136 | 80 | 0 | 3 | 138 | 5 | 140 | 1.74e-20 | 82.4 |
| MS.gene072434.t1 | AT4G04402 | 31.915 | 141 | 82 | 4 | 22 | 150 | 24 | 162 | 1.99e-18 | 77.4 |
| MS.gene072434.t1 | AT1G80100 | 38.255 | 149 | 69 | 3 | 3 | 150 | 5 | 131 | 4.94e-18 | 75.5 |
Find 20 sgRNAs with CRISPR-Local
Find 58 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGAGAAGCTTCTTAATCAAT+TGG | 0.405079 | 7.3:-2924217 | MS.gene072434:CDS |
| CTTGAAGAGTGTCTCAAACT+TGG | 0.419955 | 7.3:+2923532 | None:intergenic |
| AGTGGTCACTCTCTTCTTTG+AGG | 0.421547 | 7.3:-2924244 | MS.gene072434:CDS |
| ACCACTTCAACAACAAAGTC+AGG | 0.424359 | 7.3:+2924261 | None:intergenic |
| CAAGTTTGAGACACTCTTCA+AGG | 0.437960 | 7.3:-2923531 | MS.gene072434:intron |
| GCTGGTTAAATTGATCATCT+AGG | 0.442108 | 7.3:+2924310 | None:intergenic |
| TCCCTTATCATTCACTATCT+AGG | 0.448382 | 7.3:+2923372 | None:intergenic |
| CATCTTGAAGTAGTTGAAGC+TGG | 0.469279 | 7.3:+2924292 | None:intergenic |
| TGGAGCTGCTGCCTTTCAGC+TGG | 0.470844 | 7.3:+2924029 | None:intergenic |
| AGCTGAAAGGCAGCAGCTCC+AGG | 0.471527 | 7.3:-2924027 | MS.gene072434:intron |
| CCCTGACTTTGTTGTTGAAG+TGG | 0.477305 | 7.3:-2924262 | MS.gene072434:CDS |
| TAAATGTTACCTCTTGAAAG+AGG | 0.497593 | 7.3:+2924412 | None:intergenic |
| ATTAATCCCAAGCAGAGGCC+AGG | 0.527313 | 7.3:-2924092 | MS.gene072434:intron |
| CACTTCTTCCCTCTTTCAAG+AGG | 0.532768 | 7.3:-2924421 | MS.gene072434:intron |
| GGCTCAGTTGCAAAGACAGT+TGG | 0.573415 | 7.3:-2924451 | MS.gene072434:CDS |
| GCTCTTGTTCACCAGCTGAA+AGG | 0.590228 | 7.3:-2924040 | MS.gene072434:CDS |
| AAATGTTACCTCTTGAAAGA+GGG | 0.594954 | 7.3:+2924413 | None:intergenic |
| AAGCTTGCAAGACATAAAGC+AGG | 0.597078 | 7.3:-2923573 | MS.gene072434:CDS |
| GAGAACAACAATGTATAGTG+TGG | 0.639598 | 7.3:-2924472 | None:intergenic |
| CCACTTCAACAACAAAGTCA+GGG | 0.677112 | 7.3:+2924262 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAATTTAATACATATATGAA+GGG | + | chr7.3:2924358-2924377 | None:intergenic | 10.0% |
| !! | TTAATTTAATACATATATGA+AGG | + | chr7.3:2924359-2924378 | None:intergenic | 10.0% |
| !!! | AAAATAACATTGATTTTAAA+AGG | - | chr7.3:2923779-2923798 | MS.gene072434:intron | 10.0% |
| !!! | AAATAACATTGATTTTAAAA+GGG | - | chr7.3:2923780-2923799 | MS.gene072434:intron | 10.0% |
| !!! | TTTTCTTTATTTTATGTATA+GGG | - | chr7.3:2923691-2923710 | MS.gene072434:intron | 10.0% |
| !! | TATAGAATTATACATTACTT+TGG | + | chr7.3:2923718-2923737 | None:intergenic | 15.0% |
| !!! | AGAAAAAATATACATGTTTT+TGG | - | chr7.3:2924236-2924255 | MS.gene072434:CDS | 15.0% |
| !!! | CTTTTCTTTATTTTATGTAT+AGG | - | chr7.3:2923690-2923709 | MS.gene072434:intron | 15.0% |
| !! | AAGAAAAGATAGAAAGAAAA+TGG | + | chr7.3:2923487-2923506 | None:intergenic | 20.0% |
| !!! | CAACTTGTATAATTTTGATA+AGG | - | chr7.3:2924040-2924059 | MS.gene072434:CDS | 20.0% |
| !!! | TAAAATCAATGTTATTTTCC+TGG | + | chr7.3:2923777-2923796 | None:intergenic | 20.0% |
| !!! | TACTTTCTTATCTTGTTTTA+AGG | + | chr7.3:2924002-2924021 | None:intergenic | 20.0% |
| ! | CAAAAGTTCAATATATTACC+TGG | + | chr7.3:2923842-2923861 | None:intergenic | 25.0% |
| ! | CAATTGGAAAAAGAAATGTA+AGG | - | chr7.3:2923647-2923666 | MS.gene072434:intron | 25.0% |
| !! | CTTATCTTATTTCAAGAACA+AGG | + | chr7.3:2923944-2923963 | None:intergenic | 25.0% |
| !! | TCTATCTTTTCTTTCATTTG+TGG | - | chr7.3:2923494-2923513 | MS.gene072434:intron | 25.0% |
| !!! | TAACATTGATTTTAAAAGGG+TGG | - | chr7.3:2923783-2923802 | MS.gene072434:intron | 25.0% |
| AAATGTTACCTCTTGAAAGA+GGG | + | chr7.3:2923438-2923457 | None:intergenic | 30.0% | |
| AGAGAAGCTTCTTAATCAAT+TGG | - | chr7.3:2923631-2923650 | MS.gene072434:intron | 30.0% | |
| CTTATTTCAAGAACAAGGAA+AGG | + | chr7.3:2923939-2923958 | None:intergenic | 30.0% | |
| GTTATTGGAAAATACAGCAT+TGG | - | chr7.3:2924070-2924089 | MS.gene072434:CDS | 30.0% | |
| TAAATGTTACCTCTTGAAAG+AGG | + | chr7.3:2923439-2923458 | None:intergenic | 30.0% | |
| ! | TGTGGTGATATCTTTGAAAA+GGG | - | chr7.3:2923512-2923531 | MS.gene072434:intron | 30.0% |
| ! | TTGTGGTGATATCTTTGAAA+AGG | - | chr7.3:2923511-2923530 | MS.gene072434:intron | 30.0% |
| !!! | TATACATGTTTTTGGCATTC+AGG | - | chr7.3:2924244-2924263 | MS.gene072434:CDS | 30.0% |
| GCTGGTTAAATTGATCATCT+AGG | + | chr7.3:2923541-2923560 | None:intergenic | 35.0% | |
| GTGAGGAGAAAAACATAGAA+GGG | - | chr7.3:2924137-2924156 | MS.gene072434:intron | 35.0% | |
| TGATAAGGTCGTTGAGTTAT+TGG | - | chr7.3:2924055-2924074 | MS.gene072434:CDS | 35.0% | |
| TGTGAGGAGAAAAACATAGA+AGG | - | chr7.3:2924136-2924155 | MS.gene072434:intron | 35.0% | |
| TTCTAATTAATCCCAAGCAG+AGG | - | chr7.3:2923751-2923770 | MS.gene072434:CDS | 35.0% | |
| TTGATGTGATTAAGTCAACG+AGG | + | chr7.3:2923880-2923899 | None:intergenic | 35.0% | |
| TTTGCTTCTGTGATTGTAGA+TGG | - | chr7.3:2924421-2924440 | MS.gene072434:intron | 35.0% | |
| !! | CTCAAACTTGGTTTTCACTA+AGG | + | chr7.3:2924307-2924326 | None:intergenic | 35.0% |
| AAGCTTGCAAGACATAAAGC+AGG | - | chr7.3:2924275-2924294 | MS.gene072434:CDS | 40.0% | |
| ACCACTTCAACAACAAAGTC+AGG | + | chr7.3:2923590-2923609 | None:intergenic | 40.0% | |
| CATCTTGAAGTAGTTGAAGC+TGG | + | chr7.3:2923559-2923578 | None:intergenic | 40.0% | |
| CCACTTCAACAACAAAGTCA+GGG | + | chr7.3:2923589-2923608 | None:intergenic | 40.0% | |
| TGCCTTCAAAAACTACTGTG+AGG | - | chr7.3:2924120-2924139 | MS.gene072434:intron | 40.0% | |
| TGTGATTAAGTCAACGAGGT+CGG | + | chr7.3:2923876-2923895 | None:intergenic | 40.0% | |
| TTGAGACACTCTTCAAGGTA+AGG | - | chr7.3:2924322-2924341 | MS.gene072434:intron | 40.0% | |
| ! | CAAGTTTGAGACACTCTTCA+AGG | - | chr7.3:2924317-2924336 | MS.gene072434:CDS | 40.0% |
| ! | CTCCTCACAGTAGTTTTTGA+AGG | + | chr7.3:2924125-2924144 | None:intergenic | 40.0% |
| !! | CTTGAAGAGTGTCTCAAACT+TGG | + | chr7.3:2924319-2924338 | None:intergenic | 40.0% |
| AGTGGTCACTCTCTTCTTTG+AGG | - | chr7.3:2923604-2923623 | MS.gene072434:intron | 45.0% | |
| CACTTCTTCCCTCTTTCAAG+AGG | - | chr7.3:2923427-2923446 | MS.gene072434:intron | 45.0% | |
| ! | AGCAGCAACTTTTTGCTGCA+GGG | - | chr7.3:2924444-2924463 | MS.gene072434:CDS | 45.0% |
| ! | TATTTTCCTGGCCTCTGCTT+GGG | + | chr7.3:2923765-2923784 | None:intergenic | 45.0% |
| ! | TTATTTTCCTGGCCTCTGCT+TGG | + | chr7.3:2923766-2923785 | None:intergenic | 45.0% |
| !! | CCCTGACTTTGTTGTTGAAG+TGG | - | chr7.3:2923586-2923605 | MS.gene072434:intron | 45.0% |
| ATTAATCCCAAGCAGAGGCC+AGG | - | chr7.3:2923756-2923775 | MS.gene072434:CDS | 50.0% | |
| GCTCTTGTTCACCAGCTGAA+AGG | - | chr7.3:2923808-2923827 | MS.gene072434:intron | 50.0% | |
| GGCTCAGTTGCAAAGACAGT+TGG | - | chr7.3:2923397-2923416 | MS.gene072434:CDS | 50.0% | |
| ! | ACTTTTTGCTGCAGGGGGTT+CGG | - | chr7.3:2924451-2924470 | MS.gene072434:CDS | 50.0% |
| ! | CAGCAACTTTTTGCTGCAGG+GGG | - | chr7.3:2924446-2924465 | MS.gene072434:CDS | 50.0% |
| ! | GAGCAGCAACTTTTTGCTGC+AGG | - | chr7.3:2924443-2924462 | MS.gene072434:CDS | 50.0% |
| ! | GCAGCAACTTTTTGCTGCAG+GGG | - | chr7.3:2924445-2924464 | MS.gene072434:CDS | 50.0% |
| AGCTGAAAGGCAGCAGCTCC+AGG | - | chr7.3:2923821-2923840 | MS.gene072434:intron | 60.0% | |
| TGGAGCTGCTGCCTTTCAGC+TGG | + | chr7.3:2923822-2923841 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 2923386 | 2924484 | 2923386 | ID=MS.gene072434 |
| chr7.3 | mRNA | 2923386 | 2924484 | 2923386 | ID=MS.gene072434.t1;Parent=MS.gene072434 |
| chr7.3 | exon | 2924422 | 2924484 | 2924422 | ID=MS.gene072434.t1.exon1;Parent=MS.gene072434.t1 |
| chr7.3 | CDS | 2924422 | 2924484 | 2924422 | ID=cds.MS.gene072434.t1;Parent=MS.gene072434.t1 |
| chr7.3 | exon | 2924207 | 2924337 | 2924207 | ID=MS.gene072434.t1.exon2;Parent=MS.gene072434.t1 |
| chr7.3 | CDS | 2924207 | 2924337 | 2924207 | ID=cds.MS.gene072434.t1;Parent=MS.gene072434.t1 |
| chr7.3 | exon | 2924028 | 2924099 | 2924028 | ID=MS.gene072434.t1.exon3;Parent=MS.gene072434.t1 |
| chr7.3 | CDS | 2924028 | 2924099 | 2924028 | ID=cds.MS.gene072434.t1;Parent=MS.gene072434.t1 |
| chr7.3 | exon | 2923712 | 2923783 | 2923712 | ID=MS.gene072434.t1.exon4;Parent=MS.gene072434.t1 |
| chr7.3 | CDS | 2923712 | 2923783 | 2923712 | ID=cds.MS.gene072434.t1;Parent=MS.gene072434.t1 |
| chr7.3 | exon | 2923532 | 2923604 | 2923532 | ID=MS.gene072434.t1.exon5;Parent=MS.gene072434.t1 |
| chr7.3 | CDS | 2923532 | 2923604 | 2923532 | ID=cds.MS.gene072434.t1;Parent=MS.gene072434.t1 |
| chr7.3 | exon | 2923386 | 2923430 | 2923386 | ID=MS.gene072434.t1.exon6;Parent=MS.gene072434.t1 |
| chr7.3 | CDS | 2923386 | 2923430 | 2923386 | ID=cds.MS.gene072434.t1;Parent=MS.gene072434.t1 |
| Gene Sequence |
| Protein sequence |
