AlfalfaGEDB | MS.gene074337

M. sativa cultivar XinJiangDaYe / MS.gene074337

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene074337.t1	XP_003597234.1	93.5	139	7	2	1	138	1	138	2.50E-62	248.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene074337.t1	G7IRR9	93.5	139	7	2	1	138	1	138	1.8e-62	248.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene051719	MS.gene074337	0.825772	3.91E-54	-1.69E-46
MS.gene051720	MS.gene074337	0.823675	1.22E-53	-1.69E-46
MS.gene052516	MS.gene074337	0.81974	9.91E-53	-1.69E-46
MS.gene052732	MS.gene074337	0.814309	1.65E-51	-1.69E-46
MS.gene053144	MS.gene074337	0.870074	1.92E-66	-1.69E-46
MS.gene053581	MS.gene074337	0.81702	4.09E-52	-1.69E-46
MS.gene054584	MS.gene074337	0.865072	7.70E-65	-1.69E-46
MS.gene055144	MS.gene074337	0.821604	3.70E-53	-1.69E-46
MS.gene055465	MS.gene074337	0.802748	4.84E-49	-1.69E-46
MS.gene055537	MS.gene074337	0.812269	4.61E-51	-1.69E-46
MS.gene055580	MS.gene074337	-0.804136	2.50E-49	-1.69E-46
MS.gene055890	MS.gene074337	0.81959	1.07E-52	-1.69E-46
MS.gene055891	MS.gene074337	0.807798	4.24E-50	-1.69E-46
MS.gene056553	MS.gene074337	0.815738	7.93E-52	-1.69E-46
MS.gene058339	MS.gene074337	0.810975	8.82E-51	-1.69E-46
MS.gene058945	MS.gene074337	0.826321	2.89E-54	-1.69E-46
MS.gene05894	MS.gene074337	0.804248	2.37E-49	-1.69E-46
MS.gene060519	MS.gene074337	0.807173	5.75E-50	-1.69E-46
MS.gene060884	MS.gene074337	0.81691	4.33E-52	-1.69E-46
MS.gene060886	MS.gene074337	0.815393	9.46E-52	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene074337.t1	MTR_2g094210	93.525	139	7	2	1	138	1	138	2.17e-90	258
MS.gene074337.t1	MTR_4g059310	54.867	113	44	3	17	127	19	126	3.63e-36	121

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene074337.t1	AT2G28410	43.119	109	55	3	7	110	9	115	9.53e-23	86.7
MS.gene074337.t1	AT5G60650	32.609	138	80	4	7	133	10	145	4.30e-13	62.8

Find 28 sgRNAs with CRISPR-Local

Find 28 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GTGAAAATGCTTCAGGATTT+TGG	0.150554	2.2:-7037205	None:intergenic
GGCATTGTTGGAATTATCTT+TGG	0.299173	2.2:+7037401	MS.gene074337:CDS
AGACAAAGGTAGGAAACATT+TGG	0.333054	2.2:+7037370	MS.gene074337:CDS
TTGGGAGCTGGTGGCATTGT+TGG	0.397431	2.2:+7037389	MS.gene074337:CDS
TTGAACACTATTATTAGCTT+TGG	0.406861	2.2:-7037498	None:intergenic
TGAGTAGCTTCTACAGCTAA+AGG	0.425538	2.2:-7037320	None:intergenic
GCTGATGGTGAAAATGCTTC+AGG	0.426528	2.2:-7037212	None:intergenic
GACAAAGGTAGGAAACATTT+GGG	0.431572	2.2:+7037371	MS.gene074337:CDS
AGCTTTGGCTCTAATCATGT+TGG	0.475963	2.2:-7037483	None:intergenic
AGCTTCTACAGCTAAAGGTA+AGG	0.481895	2.2:-7037315	None:intergenic
AAAGGCAAGGTACATCAAAC+AGG	0.497433	2.2:-7037524	None:intergenic
GTGGAAAATTAGACAAAGGT+AGG	0.516928	2.2:+7037360	MS.gene074337:CDS
CTTTGGTGTTGCATTTGTTG+TGG	0.527586	2.2:+7037418	MS.gene074337:CDS
TAGTAACAAGTGATAGAGAA+AGG	0.528456	2.2:-7037142	None:intergenic
GGTAGGAAACATTTGGGAGC+TGG	0.541255	2.2:+7037377	MS.gene074337:CDS
CAAATTCATGAAAGCAAAGG+TGG	0.545860	2.2:+7037341	MS.gene074337:CDS
CATCCCAATTCTCAAAAGCA+TGG	0.558982	2.2:+7037248	MS.gene074337:CDS
GCTTCTACAGCTAAAGGTAA+GGG	0.562499	2.2:-7037314	None:intergenic
TCTCAAGAAAGAGCACCTCA+TGG	0.585347	2.2:+7037173	MS.gene074337:CDS
ACTCAAATTCATGAAAGCAA+AGG	0.598332	2.2:+7037338	MS.gene074337:CDS
AAGAAAATGATCAATCACAT+TGG	0.602394	2.2:+7037110	None:intergenic
TAACAAGTGATAGAGAAAGG+AGG	0.603025	2.2:-7037139	None:intergenic
GAACATTTAGATGAAGTGCA+TGG	0.610364	2.2:-7037290	None:intergenic
TCTAACATGATACACACTCA+TGG	0.618148	2.2:-7037447	None:intergenic
TGAAAGAATTCATATGCTGA+TGG	0.627715	2.2:-7037227	None:intergenic
AGGAAACATTTGGGAGCTGG+TGG	0.627993	2.2:+7037380	MS.gene074337:CDS
AAAGGTGGAAAATTAGACAA+AGG	0.635337	2.2:+7037356	MS.gene074337:CDS
GGCAAGGTACATCAAACAGG+TGG	0.689975	2.2:-7037521	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TTGAACACTATTATTAGCTT+TGG	-	chr2.2:7037501-7037520	None:intergenic	25.0%
	AAAGGTGGAAAATTAGACAA+AGG	+	chr2.2:7037356-7037375	MS.gene074337:CDS	30.0%
	ACTCAAATTCATGAAAGCAA+AGG	+	chr2.2:7037338-7037357	MS.gene074337:CDS	30.0%
	TAGTAACAAGTGATAGAGAA+AGG	-	chr2.2:7037145-7037164	None:intergenic	30.0%
	TGAAAGAATTCATATGCTGA+TGG	-	chr2.2:7037230-7037249	None:intergenic	30.0%
!!	ATTACCATGCTTTTGAGAAT+TGG	-	chr2.2:7037255-7037274	None:intergenic	30.0%
!!	TTACCATGCTTTTGAGAATT+GGG	-	chr2.2:7037254-7037273	None:intergenic	30.0%
	AGACAAAGGTAGGAAACATT+TGG	+	chr2.2:7037370-7037389	MS.gene074337:CDS	35.0%
	CAAATTCATGAAAGCAAAGG+TGG	+	chr2.2:7037341-7037360	MS.gene074337:CDS	35.0%
	GAACATTTAGATGAAGTGCA+TGG	-	chr2.2:7037293-7037312	None:intergenic	35.0%
	GACAAAGGTAGGAAACATTT+GGG	+	chr2.2:7037371-7037390	MS.gene074337:CDS	35.0%
	GGCATTGTTGGAATTATCTT+TGG	+	chr2.2:7037401-7037420	MS.gene074337:CDS	35.0%
	GTGGAAAATTAGACAAAGGT+AGG	+	chr2.2:7037360-7037379	MS.gene074337:CDS	35.0%
	TAACAAGTGATAGAGAAAGG+AGG	-	chr2.2:7037142-7037161	None:intergenic	35.0%
	TCTAACATGATACACACTCA+TGG	-	chr2.2:7037450-7037469	None:intergenic	35.0%
!	GTGAAAATGCTTCAGGATTT+TGG	-	chr2.2:7037208-7037227	None:intergenic	35.0%
!!	TTTTGGTTAACAAGTCCATG+AGG	-	chr2.2:7037191-7037210	None:intergenic	35.0%
	AGCTTCTACAGCTAAAGGTA+AGG	-	chr2.2:7037318-7037337	None:intergenic	40.0%
	CATCCCAATTCTCAAAAGCA+TGG	+	chr2.2:7037248-7037267	MS.gene074337:CDS	40.0%
	GCTTCTACAGCTAAAGGTAA+GGG	-	chr2.2:7037317-7037336	None:intergenic	40.0%
	TGAGTAGCTTCTACAGCTAA+AGG	-	chr2.2:7037323-7037342	None:intergenic	40.0%
!!	AGCTTTGGCTCTAATCATGT+TGG	-	chr2.2:7037486-7037505	None:intergenic	40.0%
!!	CTTTGGTGTTGCATTTGTTG+TGG	+	chr2.2:7037418-7037437	MS.gene074337:CDS	40.0%
!	GCTGATGGTGAAAATGCTTC+AGG	-	chr2.2:7037215-7037234	None:intergenic	45.0%
!!	TCTCAAGAAAGAGCACCTCA+TGG	+	chr2.2:7037173-7037192	MS.gene074337:CDS	45.0%
	AGGAAACATTTGGGAGCTGG+TGG	+	chr2.2:7037380-7037399	MS.gene074337:CDS	50.0%
	GGTAGGAAACATTTGGGAGC+TGG	+	chr2.2:7037377-7037396	MS.gene074337:CDS	50.0%
	TTGGGAGCTGGTGGCATTGT+TGG	+	chr2.2:7037389-7037408	MS.gene074337:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.2	gene	7037116	7037532	7037116	ID=MS.gene074337
chr2.2	mRNA	7037116	7037532	7037116	ID=MS.gene074337.t1;Parent=MS.gene074337
chr2.2	exon	7037116	7037532	7037116	ID=MS.gene074337.t1.exon1;Parent=MS.gene074337.t1
chr2.2	CDS	7037116	7037532	7037116	ID=cds.MS.gene074337.t1;Parent=MS.gene074337.t1

Gene Sequence

>MS.gene074337

ATGATCAATCACATTGGTTTCTTCCTCCTTTCTCTATCACTTGTTACTATTGTTACATCTCAAGAAAGAGCACCTCATGGACTTGTTAACCAAAATCCTGAAGCATTTTCACCATCAGCATATGAATTCTTTCATCCCAATTCTCAAAAGCATGGTAATGACAAAAAAGATGATCCATGCACTTCATCTAAATGTTCACCCTTACCTTTAGCTGTAGAAGCTACTCAAATTCATGAAAGCAAAGGTGGAAAATTAGACAAAGGTAGGAAACATTTGGGAGCTGGTGGCATTGTTGGAATTATCTTTGGTGTTGCATTTGTTGTGGTTTTAGCCATGAGTGTGTATCATGTTAGAGTCACACGTAAAGCCAACATGATTAGAGCCAAAGCTAATAATAGTGTTCAACCACCTGTTTGA

Protein sequence

>MS.gene074337.t1

MINHIGFFLLSLSLVTIVTSQERAPHGLVNQNPEAFSPSAYEFFHPNSQKHGNDKKDDPCTSSKCSPLPLAVEATQIHESKGGKLDKGRKHLGAGGIVGIIFGVAFVVVLAMSVYHVRVTRKANMIRAKANNSVQPPV