AlfalfaGEDB | MS.gene08727

M. sativa cultivar XinJiangDaYe / MS.gene08727

KEGG: K12881
GO: GO:0003674 GO:0003676 GO:0005488 GO:0097159 GO:1901363
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene08727.t1	XP_024637923.1	98.3	60	1	0	1	60	135	194	1.30E-23	118.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene08727.t1	Q8L773	72.9	59	15	1	1	59	129	186	5.4e-15	80.9

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene08727.t1	A0A072UK32	98.3	60	1	0	1	60	135	194	9.2e-24	118.2

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049887	MS.gene08727	0.823362	1.44E-53	-1.69E-46
MS.gene050384	MS.gene08727	0.80762	4.62E-50	-1.69E-46
MS.gene050386	MS.gene08727	0.813808	2.12E-51	-1.69E-46
MS.gene050551	MS.gene08727	0.81028	1.25E-50	-1.69E-46
MS.gene050689	MS.gene08727	0.854433	1.24E-61	-1.69E-46
MS.gene050838	MS.gene08727	0.848933	4.47E-60	-1.69E-46
MS.gene051102	MS.gene08727	0.813191	2.90E-51	-1.69E-46
MS.gene051328	MS.gene08727	0.802202	6.27E-49	-1.69E-46
MS.gene051343	MS.gene08727	0.824681	7.08E-54	-1.69E-46
MS.gene051381	MS.gene08727	0.813587	2.37E-51	-1.69E-46
MS.gene051642	MS.gene08727	0.806993	6.28E-50	-1.69E-46
MS.gene052133	MS.gene08727	0.801726	7.86E-49	-1.69E-46
MS.gene052307	MS.gene08727	0.826602	2.48E-54	-1.69E-46
MS.gene052335	MS.gene08727	0.800048	1.73E-48	-1.69E-46
MS.gene052397	MS.gene08727	0.832017	1.20E-55	-1.69E-46
MS.gene052516	MS.gene08727	0.802161	6.40E-49	-1.69E-46
MS.gene052609	MS.gene08727	0.820756	5.80E-53	-1.69E-46
MS.gene055311	MS.gene08727	0.814295	1.66E-51	-1.69E-46
MS.gene055313	MS.gene08727	0.802007	6.88E-49	-1.69E-46
MS.gene055512	MS.gene08727	0.808672	2.76E-50	-1.69E-46
MS.gene056181	MS.gene08727	0.805095	1.57E-49	-1.69E-46
MS.gene056308	MS.gene08727	0.804496	2.10E-49	-1.69E-46
MS.gene056432	MS.gene08727	0.804467	2.13E-49	-1.69E-46
MS.gene057057	MS.gene08727	0.822476	2.32E-53	-1.69E-46
MS.gene057234	MS.gene08727	0.80818	3.52E-50	-1.69E-46
MS.gene058055	MS.gene08727	0.806513	7.93E-50	-1.69E-46
MS.gene058638	MS.gene08727	0.803418	3.52E-49	-1.69E-46
MS.gene058639	MS.gene08727	0.811031	8.58E-51	-1.69E-46
MS.gene058852	MS.gene08727	0.802821	4.68E-49	-1.69E-46
MS.gene058906	MS.gene08727	0.816177	6.33E-52	-1.69E-46
MS.gene059171	MS.gene08727	0.842589	2.36E-58	-1.69E-46
MS.gene05978	MS.gene08727	0.827808	1.27E-54	-1.69E-46
MS.gene061006	MS.gene08727	0.82073	5.88E-53	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene08727.t1	MTR_4g063557	98.333	60	1	1	60	135	194	1.78e-35	120
MS.gene08727.t1	MTR_5g084370	67.308	52	17	1	52	98	149	1.65e-17	72.0
MS.gene08727.t1	MTR_5g041970	71.111	45	13	1	45	22	66	4.02e-17	68.2
MS.gene08727.t1	MTR_3g065490	72.340	47	13	1	47	150	196	1.05e-16	71.2
MS.gene08727.t1	MTR_7g024090	57.692	52	22	1	52	116	167	7.30e-16	69.3
MS.gene08727.t1	MTR_3g118240	78.947	38	8	1	38	118	155	6.63e-15	65.9
MS.gene08727.t1	MTR_7g024150	53.488	43	20	1	43	51	93	6.42e-12	55.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene08727.t1	AT5G59950	72.881	59	15	1	1	59	63	120	5.51e-22	83.2
MS.gene08727.t1	AT5G59950	72.881	59	15	1	1	59	129	186	1.01e-21	83.2
MS.gene08727.t1	AT5G59950	72.881	59	15	1	1	59	127	184	7.23e-20	79.0
MS.gene08727.t1	AT5G59950	72.881	59	15	1	1	59	129	186	8.38e-20	79.0
MS.gene08727.t1	AT5G59950	72.881	59	15	1	1	59	129	186	9.16e-20	79.0
MS.gene08727.t1	AT5G02530	78.431	51	10	1	1	51	149	198	1.75e-19	79.0
MS.gene08727.t1	AT5G02530	78.431	51	10	1	1	51	149	198	1.89e-19	79.0
MS.gene08727.t1	AT1G66260	55.102	49	22	0	1	49	148	196	6.68e-13	61.2
MS.gene08727.t1	AT1G66260	55.102	49	22	0	1	49	148	196	6.68e-13	61.2
MS.gene08727.t1	AT5G37720	53.333	60	23	2	1	57	134	191	7.57e-13	60.8
MS.gene08727.t1	AT5G37720	53.333	60	23	2	1	57	134	191	7.90e-13	60.8

Find 13 sgRNAs with CRISPR-Local

Find 15 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TACAACAATGTTCAACTAGA+TGG	0.304919	4.2:-75312130	MS.gene08727:CDS
CCAACAATCTCTATCTTCAT+TGG	0.329913	4.2:+75312103	None:intergenic
CCAATGGCTGCATTTACAAC+AGG	0.420547	4.2:+75312052	None:intergenic
CAAGCCACTGAATACCTTTG+AGG	0.471394	4.2:+75312013	None:intergenic
CCAATGAAGATAGAGATTGT+TGG	0.485750	4.2:-75312103	MS.gene08727:CDS
CCTGTTGTAAATGCAGCCAT+TGG	0.541269	4.2:-75312052	MS.gene08727:CDS
AATTCCATTAAAATTTCCAA+TGG	0.541998	4.2:+75312036	None:intergenic
ACAACAATGTTCAACTAGAT+GGG	0.548777	4.2:-75312129	MS.gene08727:CDS
TGCACCAGGTGTAGATATGT+TGG	0.555986	4.2:+75312078	None:intergenic
TTTACAACAGGAGCTGCACC+AGG	0.606543	4.2:+75312064	None:intergenic
CCGCAGAAGTAGTCTTCTCA+CGG	0.626643	4.2:-75312183	MS.gene08727:CDS
GGAACCAACATATCTACACC+TGG	0.632548	4.2:-75312082	MS.gene08727:CDS
CCGTGAGAAGACTACTTCTG+CGG	0.658109	4.2:+75312183	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AATTCCATTAAAATTTCCAA+TGG	+	chr4.2:75312182-75312201	None:intergenic	20.0%
!!	TTTTAATGGAATTCCTCAAA+GGG	-	chr4.2:75312189-75312208	MS.gene08727:CDS	25.0%
!!!	ATTTTAATGGAATTCCTCAA+AGG	-	chr4.2:75312188-75312207	MS.gene08727:CDS	25.0%
	ACAACAATGTTCAACTAGAT+GGG	-	chr4.2:75312086-75312105	MS.gene08727:CDS	30.0%
	TACAACAATGTTCAACTAGA+TGG	-	chr4.2:75312085-75312104	MS.gene08727:CDS	30.0%
	CCAACAATCTCTATCTTCAT+TGG	+	chr4.2:75312115-75312134	None:intergenic	35.0%
	CCAATGAAGATAGAGATTGT+TGG	-	chr4.2:75312112-75312131	MS.gene08727:CDS	35.0%
!	GCAGCCATTGGAAATTTTAA+TGG	-	chr4.2:75312175-75312194	MS.gene08727:CDS	35.0%
	CCAATGGCTGCATTTACAAC+AGG	+	chr4.2:75312166-75312185	None:intergenic	45.0%
	CCTGTTGTAAATGCAGCCAT+TGG	-	chr4.2:75312163-75312182	MS.gene08727:CDS	45.0%
	GGAACCAACATATCTACACC+TGG	-	chr4.2:75312133-75312152	MS.gene08727:CDS	45.0%
	TGCACCAGGTGTAGATATGT+TGG	+	chr4.2:75312140-75312159	None:intergenic	45.0%
	CCGCAGAAGTAGTCTTCTCA+CGG	-	chr4.2:75312032-75312051	MS.gene08727:CDS	50.0%
	CCGTGAGAAGACTACTTCTG+CGG	+	chr4.2:75312035-75312054	None:intergenic	50.0%
	TTTACAACAGGAGCTGCACC+AGG	+	chr4.2:75312154-75312173	None:intergenic	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.2	gene	75312028	75312209	75312028	ID=MS.gene08727
chr4.2	mRNA	75312028	75312209	75312028	ID=MS.gene08727.t1;Parent=MS.gene08727
chr4.2	exon	75312028	75312209	75312028	ID=MS.gene08727.t1.exon1;Parent=MS.gene08727.t1
chr4.2	CDS	75312028	75312209	75312028	ID=cds.MS.gene08727.t1;Parent=MS.gene08727.t1

Gene Sequence	>MS.gene08727 GGTACCGCAGAAGTAGTCTTCTCACGGAGACAAGATGCTGTAGCTGCTGTGAAGAGATACAACAATGTTCAACTAGATGGGAAGCCAATGAAGATAGAGATTGTTGGAACCAACATATCTACACCTGGTGCAGCTCCTGTTGTAAATGCAGCCATTGGAAATTTTAATGGAATTCCTCAAAG
Protein sequence	>MS.gene08727.t1 GTAEVVFSRRQDAVAAVKRYNNVQLDGKPMKIEIVGTNISTPGAAPVVNAAIGNFNGIPQ