AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene08966


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene08966.t1 MTR_8g081600 94.096 271 16 0 1 271 1 271 0.0 523
MS.gene08966.t1 MTR_2g036780 36.162 271 156 6 1 260 164 428 1.50e-43 153
MS.gene08966.t1 MTR_1g035890 33.582 268 165 5 1 260 165 427 1.23e-41 148
MS.gene08966.t1 MTR_3g100150 32.222 270 171 6 1 261 163 429 1.47e-40 146
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene08966.t1 AT3G23840 39.179 268 146 8 1 266 164 416 6.32e-46 159
MS.gene08966.t1 AT4G13840 33.457 269 168 6 1 266 166 426 6.11e-43 151
MS.gene08966.t1 AT4G24510 30.418 263 166 6 1 260 168 416 9.88e-38 137

Find 74 sgRNAs with CRISPR-Local

Find 92 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACAACTTGCAAGAACAAATT+TGG 0.140491 4.4:-14808676 MS.gene08966:CDS
TGACACTAGTTTGAAAATTT+TGG 0.151455 4.4:-14808456 MS.gene08966:CDS
GTCTCAACACCCCTTAATTT+TGG 0.157335 4.4:-14808815 MS.gene08966:CDS
AAACTTTATGTGCAATAATT+TGG 0.180735 4.4:-14808622 MS.gene08966:CDS
CTTCAGATTTCATCAACTTA+TGG 0.205690 4.4:-14808892 MS.gene08966:CDS
GTTCTTGCAAGTTGTTCATT+AGG 0.260077 4.4:+14808684 None:intergenic
TTCAGATTTCATCAACTTAT+GGG 0.298539 4.4:-14808891 MS.gene08966:CDS
TGGTAGGGGCAATGATATAA+TGG 0.301115 4.4:-14808531 MS.gene08966:CDS
TGATGAAAGAAATCAAATTA+TGG 0.305939 4.4:-14808408 MS.gene08966:CDS
ATATCATTGCCCCTACCATT+AGG 0.319594 4.4:+14808536 None:intergenic
TTCTTGCAAGTTGTTCATTA+GGG 0.331603 4.4:+14808685 None:intergenic
TGTGTATGACTTAAAATTAA+AGG 0.335448 4.4:-14808303 MS.gene08966:CDS
GGGAGATGAAGGTGTTGTTT+TGG 0.345479 4.4:-14808240 MS.gene08966:CDS
TTCATCTTCTGGCAATATAA+TGG 0.352304 4.4:+14808178 None:intergenic
TTGACCCGGTTGGTGATCAT+TGG 0.353117 4.4:-14808760 MS.gene08966:CDS
CAACTTGCAAGAACAAATTT+GGG 0.358829 4.4:-14808675 MS.gene08966:CDS
TATCATTGCCCCTACCATTA+GGG 0.360008 4.4:+14808537 None:intergenic
AACTGTCACGGTTGTGGGTT+CGG 0.365252 4.4:+14808571 None:intergenic
AGGAACCAGCTACATTTAAA+CGG 0.370152 4.4:-14808784 MS.gene08966:CDS
TAAGTCAAGAAATGTCAAAT+TGG 0.372854 4.4:+14808337 None:intergenic
AGGGGTGTTGAGACGGATCT+TGG 0.389004 4.4:+14808824 None:intergenic
TATATTGCCAGAAGATGAAA+TGG 0.399380 4.4:-14808174 MS.gene08966:CDS
TTTGTTTACTACACACTTCA+AGG 0.403996 4.4:-14808266 MS.gene08966:CDS
AGTGTGTAGTAAACAAATCT+TGG 0.405045 4.4:+14808272 None:intergenic
GTGTATGACTTAAAATTAAA+GGG 0.419239 4.4:-14808302 MS.gene08966:CDS
GTTCGGATCCTTCTCGAACT+CGG 0.421086 4.4:+14808588 None:intergenic
TCGACAAACTGTCACGGTTG+TGG 0.425168 4.4:+14808565 None:intergenic
GGTAGGGGCAATGATATAAT+GGG 0.427066 4.4:-14808530 MS.gene08966:CDS
ATTGCACATAAAGTTTCAAA+TGG 0.442525 4.4:+14808629 None:intergenic
CACATAAAGTTTCAAATGGA+GGG 0.442722 4.4:+14808633 None:intergenic
CGACAAACTGTCACGGTTGT+GGG 0.445047 4.4:+14808566 None:intergenic
GGAACCAGCTACATTTAAAC+GGG 0.448779 4.4:-14808783 MS.gene08966:CDS
CCTAAGGTGAATGATATTGA+TGG 0.452041 4.4:-14808212 MS.gene08966:CDS
TTCGGATCCTTCTCGAACTC+GGG 0.459230 4.4:+14808589 None:intergenic
CCTCAACCTTGCATATCAAT+TGG 0.459576 4.4:+14808501 None:intergenic
GCTAGCATGCTTGTGGATCA+AGG 0.466138 4.4:-14808434 MS.gene08966:CDS
AAATCCGAGCTTAATGTGTA+TGG 0.468103 4.4:-14808143 MS.gene08966:CDS
CTTCAAGGGGTGGGAGATGA+AGG 0.469388 4.4:-14808251 MS.gene08966:CDS
CCATCAATATCATTCACCTT+AGG 0.474137 4.4:+14808212 None:intergenic
GGGATCCAATGATCACCAAC+CGG 0.475921 4.4:+14808755 None:intergenic
GACGGATCTTGGAATGTTGA+AGG 0.485139 4.4:+14808835 None:intergenic
GGAGGGGTTTGTTCAACACT+TGG 0.494342 4.4:+14808650 None:intergenic
TACATTTAAACGGGTTGACC+CGG 0.497184 4.4:-14808774 MS.gene08966:CDS
GTTTGTCGACCCGACCCTAA+TGG 0.497568 4.4:-14808551 MS.gene08966:CDS
TCAACCCGTTTAAATGTAGC+TGG 0.502437 4.4:+14808779 None:intergenic
TTGATATGCAAGGTTGAGGC+AGG 0.515574 4.4:-14808497 MS.gene08966:CDS
GCACATAAAGTTTCAAATGG+AGG 0.536084 4.4:+14808632 None:intergenic
TACTACACACTTCAAGGGGT+GGG 0.539191 4.4:-14808260 MS.gene08966:CDS
TCGACCCGACCCTAATGGTA+GGG 0.545566 4.4:-14808546 MS.gene08966:CDS
TTGTTTACTACACACTTCAA+GGG 0.547045 4.4:-14808265 MS.gene08966:CDS
GTGTATGGTATCATGTTTGA+GGG 0.549776 4.4:-14808128 MS.gene08966:CDS
TTGCCCCTACCATTAGGGTC+GGG 0.551150 4.4:+14808542 None:intergenic
ACATAAAGTTTCAAATGGAG+GGG 0.558070 4.4:+14808634 None:intergenic
ATTGCCCCTACCATTAGGGT+CGG 0.561243 4.4:+14808541 None:intergenic
TGTGTATGGTATCATGTTTG+AGG 0.561998 4.4:-14808129 MS.gene08966:CDS
AAAATTAAGGGGTGTTGAGA+CGG 0.562724 4.4:+14808817 None:intergenic
CCAATTGATATGCAAGGTTG+AGG 0.574004 4.4:-14808501 MS.gene08966:CDS
TCAGATTTCATCAACTTATG+GGG 0.579204 4.4:-14808890 MS.gene08966:CDS
TCATTAGGGAGCTAGTTATG+TGG 0.584575 4.4:+14808699 None:intergenic
ATGAAATCTGAAGCTGAATG+TGG 0.593595 4.4:+14808902 None:intergenic
GTCGACCCGACCCTAATGGT+AGG 0.594252 4.4:-14808547 MS.gene08966:CDS
TTTAAACGGGTTGACCCGGT+TGG 0.594712 4.4:-14808770 MS.gene08966:CDS
GGATCCAATGATCACCAACC+GGG 0.601111 4.4:+14808756 None:intergenic
TTACTACACACTTCAAGGGG+TGG 0.606615 4.4:-14808261 MS.gene08966:CDS
CGACCCGACCCTAATGGTAG+GGG 0.607530 4.4:-14808545 MS.gene08966:CDS
ACGGATCTTGGAATGTTGAA+GGG 0.614481 4.4:+14808836 None:intergenic
TGTTTACTACACACTTCAAG+GGG 0.616578 4.4:-14808264 MS.gene08966:CDS
TAGGGAGCTAGTTATGTGGA+AGG 0.620633 4.4:+14808703 None:intergenic
TGCATAGCCCGAGTTCGAGA+AGG 0.625715 4.4:-14808596 MS.gene08966:CDS
GATACCATACACATTAAGCT+CGG 0.633143 4.4:+14808139 None:intergenic
AAATGACCAATTGATATGCA+AGG 0.646231 4.4:-14808507 MS.gene08966:CDS
AACTTGCAAGAACAAATTTG+GGG 0.669322 4.4:-14808674 MS.gene08966:CDS
GTCGGGTCGACAAACTGTCA+CGG 0.691405 4.4:+14808559 None:intergenic
TGAAATCTGAAGCTGAATGT+GGG 0.702970 4.4:+14808903 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAACTTTATGTGCAATAATT+TGG - chr4.4:14808407-14808426 MS.gene08966:CDS 20.0%
!! ATATAGTAGAAAAAGATCAA+GGG - chr4.4:14808644-14808663 MS.gene08966:CDS 20.0%
!! GTGTATGACTTAAAATTAAA+GGG - chr4.4:14808727-14808746 MS.gene08966:CDS 20.0%
!! TGATGAAAGAAATCAAATTA+TGG - chr4.4:14808621-14808640 MS.gene08966:CDS 20.0%
!! TGTGTATGACTTAAAATTAA+AGG - chr4.4:14808726-14808745 MS.gene08966:CDS 20.0%
! ATTGCACATAAAGTTTCAAA+TGG + chr4.4:14808403-14808422 None:intergenic 25.0%
! GATATAGTAGAAAAAGATCA+AGG - chr4.4:14808643-14808662 MS.gene08966:CDS 25.0%
! TAAGTCAAGAAATGTCAAAT+TGG + chr4.4:14808695-14808714 None:intergenic 25.0%
! TATAGTAGAAAAAGATCAAG+GGG - chr4.4:14808645-14808664 MS.gene08966:CDS 25.0%
! TTCAGATTTCATCAACTTAT+GGG - chr4.4:14808138-14808157 MS.gene08966:CDS 25.0%
!!! TGACACTAGTTTGAAAATTT+TGG - chr4.4:14808573-14808592 MS.gene08966:CDS 25.0%
AAATGACCAATTGATATGCA+AGG - chr4.4:14808522-14808541 MS.gene08966:CDS 30.0%
AACTTGCAAGAACAAATTTG+GGG - chr4.4:14808355-14808374 MS.gene08966:CDS 30.0%
ACAACTTGCAAGAACAAATT+TGG - chr4.4:14808353-14808372 MS.gene08966:CDS 30.0%
ACATAAAGTTTCAAATGGAG+GGG + chr4.4:14808398-14808417 None:intergenic 30.0%
AGTGTGTAGTAAACAAATCT+TGG + chr4.4:14808760-14808779 None:intergenic 30.0%
CAACTTGCAAGAACAAATTT+GGG - chr4.4:14808354-14808373 MS.gene08966:CDS 30.0%
CACATAAAGTTTCAAATGGA+GGG + chr4.4:14808399-14808418 None:intergenic 30.0%
CTTCAGATTTCATCAACTTA+TGG - chr4.4:14808137-14808156 MS.gene08966:CDS 30.0%
TATATTGCCAGAAGATGAAA+TGG - chr4.4:14808855-14808874 MS.gene08966:CDS 30.0%
TCAGATTTCATCAACTTATG+GGG - chr4.4:14808139-14808158 MS.gene08966:CDS 30.0%
TTCATCTTCTGGCAATATAA+TGG + chr4.4:14808854-14808873 None:intergenic 30.0%
TTCTTGCAAGTTGTTCATTA+GGG + chr4.4:14808347-14808366 None:intergenic 30.0%
TTGTTTACTACACACTTCAA+GGG - chr4.4:14808764-14808783 MS.gene08966:CDS 30.0%
TTTGTTTACTACACACTTCA+AGG - chr4.4:14808763-14808782 MS.gene08966:CDS 30.0%
! AGTTTTTCCATTTCATCTTC+TGG + chr4.4:14808865-14808884 None:intergenic 30.0%
! CTTTTCGGATCCAAAATTAA+GGG + chr4.4:14808227-14808246 None:intergenic 30.0%
! TTTTAGGCTCAAATTGTTCA+AGG + chr4.4:14808170-14808189 None:intergenic 30.0%
! TTTTCGGATCCAAAATTAAG+GGG + chr4.4:14808226-14808245 None:intergenic 30.0%
!!! GTGACTGATTTTTTTGTCTA+TGG - chr4.4:14808667-14808686 MS.gene08966:CDS 30.0%
!!! TGTGTCCATTTTTTTGTTGT+TGG + chr4.4:14808302-14808321 None:intergenic 30.0%
AAATCCGAGCTTAATGTGTA+TGG - chr4.4:14808886-14808905 MS.gene08966:CDS 35.0%
AGGAACCAGCTACATTTAAA+CGG - chr4.4:14808245-14808264 MS.gene08966:CDS 35.0%
CCATCAATATCATTCACCTT+AGG + chr4.4:14808820-14808839 None:intergenic 35.0%
CCTAAGGTGAATGATATTGA+TGG - chr4.4:14808817-14808836 MS.gene08966:CDS 35.0%
GATACCATACACATTAAGCT+CGG + chr4.4:14808893-14808912 None:intergenic 35.0%
GCACATAAAGTTTCAAATGG+AGG + chr4.4:14808400-14808419 None:intergenic 35.0%
GTGTATGGTATCATGTTTGA+GGG - chr4.4:14808901-14808920 MS.gene08966:CDS 35.0%
GTTCTTGCAAGTTGTTCATT+AGG + chr4.4:14808348-14808367 None:intergenic 35.0%
TGTGTATGGTATCATGTTTG+AGG - chr4.4:14808900-14808919 MS.gene08966:CDS 35.0%
TGTTTACTACACACTTCAAG+GGG - chr4.4:14808765-14808784 MS.gene08966:CDS 35.0%
! ATGAAATCTGAAGCTGAATG+TGG + chr4.4:14808130-14808149 None:intergenic 35.0%
! CCTTAATTTTGGATCCGAAA+AGG - chr4.4:14808225-14808244 MS.gene08966:CDS 35.0%
! CCTTTTCGGATCCAAAATTA+AGG + chr4.4:14808228-14808247 None:intergenic 35.0%
! TGAAATCTGAAGCTGAATGT+GGG + chr4.4:14808129-14808148 None:intergenic 35.0%
!! AAAATTAAGGGGTGTTGAGA+CGG + chr4.4:14808215-14808234 None:intergenic 35.0%
!! AAATGTAGCTGGTTCCTTTT+CGG + chr4.4:14808242-14808261 None:intergenic 35.0%
!!! CCATTTTTTTGTTGTTGGCT+GGG + chr4.4:14808297-14808316 None:intergenic 35.0%
!!! TCCATTTTTTTGTTGTTGGC+TGG + chr4.4:14808298-14808317 None:intergenic 35.0%
ACGGATCTTGGAATGTTGAA+GGG + chr4.4:14808196-14808215 None:intergenic 40.0%
ATATCATTGCCCCTACCATT+AGG + chr4.4:14808496-14808515 None:intergenic 40.0%
CCAATTGATATGCAAGGTTG+AGG - chr4.4:14808528-14808547 MS.gene08966:CDS 40.0%
CCCAGCCAACAACAAAAAAA+TGG - chr4.4:14808294-14808313 MS.gene08966:CDS 40.0%
CCTCAACCTTGCATATCAAT+TGG + chr4.4:14808531-14808550 None:intergenic 40.0%
GGAACCAGCTACATTTAAAC+GGG - chr4.4:14808246-14808265 MS.gene08966:CDS 40.0%
GGTAGGGGCAATGATATAAT+GGG - chr4.4:14808499-14808518 MS.gene08966:CDS 40.0%
TATCATTGCCCCTACCATTA+GGG + chr4.4:14808495-14808514 None:intergenic 40.0%
TCAACCCGTTTAAATGTAGC+TGG + chr4.4:14808253-14808272 None:intergenic 40.0%
TCATTAGGGAGCTAGTTATG+TGG + chr4.4:14808333-14808352 None:intergenic 40.0%
TGGTAGGGGCAATGATATAA+TGG - chr4.4:14808498-14808517 MS.gene08966:CDS 40.0%
! GAATGTTGAAGGGCAGTTTT+AGG + chr4.4:14808186-14808205 None:intergenic 40.0%
! GTCTCAACACCCCTTAATTT+TGG - chr4.4:14808214-14808233 MS.gene08966:CDS 40.0%
!! TACATTTAAACGGGTTGACC+CGG - chr4.4:14808255-14808274 MS.gene08966:CDS 40.0%
!!! AATTTTGGCTAGCATGCTTG+TGG - chr4.4:14808588-14808607 MS.gene08966:CDS 40.0%
!!! TGTTGTTTTGGTGCTTCCTA+AGG - chr4.4:14808801-14808820 MS.gene08966:CDS 40.0%
GACGGATCTTGGAATGTTGA+AGG + chr4.4:14808197-14808216 None:intergenic 45.0%
TACTACACACTTCAAGGGGT+GGG - chr4.4:14808769-14808788 MS.gene08966:CDS 45.0%
TAGGGAGCTAGTTATGTGGA+AGG + chr4.4:14808329-14808348 None:intergenic 45.0%
TTACTACACACTTCAAGGGG+TGG - chr4.4:14808768-14808787 MS.gene08966:CDS 45.0%
TTGATATGCAAGGTTGAGGC+AGG - chr4.4:14808532-14808551 MS.gene08966:CDS 45.0%
!!! GGGAGATGAAGGTGTTGTTT+TGG - chr4.4:14808789-14808808 MS.gene08966:CDS 45.0%
AACTGTCACGGTTGTGGGTT+CGG + chr4.4:14808461-14808480 None:intergenic 50.0%
ATTGCCCCTACCATTAGGGT+CGG + chr4.4:14808491-14808510 None:intergenic 50.0%
CGACAAACTGTCACGGTTGT+GGG + chr4.4:14808466-14808485 None:intergenic 50.0%
GCTAGCATGCTTGTGGATCA+AGG - chr4.4:14808595-14808614 MS.gene08966:CDS 50.0%
GGATCCAATGATCACCAACC+GGG + chr4.4:14808276-14808295 None:intergenic 50.0%
GGGATCCAATGATCACCAAC+CGG + chr4.4:14808277-14808296 None:intergenic 50.0%
GTTCGGATCCTTCTCGAACT+CGG + chr4.4:14808444-14808463 None:intergenic 50.0%
TCGACAAACTGTCACGGTTG+TGG + chr4.4:14808467-14808486 None:intergenic 50.0%
TTCGGATCCTTCTCGAACTC+GGG + chr4.4:14808443-14808462 None:intergenic 50.0%
! GGAGGGGTTTGTTCAACACT+TGG + chr4.4:14808382-14808401 None:intergenic 50.0%
!! TTGACCCGGTTGGTGATCAT+TGG - chr4.4:14808269-14808288 MS.gene08966:CDS 50.0%
!! TTTAAACGGGTTGACCCGGT+TGG - chr4.4:14808259-14808278 MS.gene08966:CDS 50.0%
CTTCAAGGGGTGGGAGATGA+AGG - chr4.4:14808778-14808797 MS.gene08966:CDS 55.0%
GTCGGGTCGACAAACTGTCA+CGG + chr4.4:14808473-14808492 None:intergenic 55.0%
GTTTGTCGACCCGACCCTAA+TGG - chr4.4:14808478-14808497 MS.gene08966:CDS 55.0%
TCGACCCGACCCTAATGGTA+GGG - chr4.4:14808483-14808502 MS.gene08966:CDS 55.0%
TGCATAGCCCGAGTTCGAGA+AGG - chr4.4:14808433-14808452 MS.gene08966:CDS 55.0%
TTGCCCCTACCATTAGGGTC+GGG + chr4.4:14808490-14808509 None:intergenic 55.0%
!! AGGGGTGTTGAGACGGATCT+TGG + chr4.4:14808208-14808227 None:intergenic 55.0%
CGACCCGACCCTAATGGTAG+GGG - chr4.4:14808484-14808503 MS.gene08966:CDS 60.0%
GTCGACCCGACCCTAATGGT+AGG - chr4.4:14808482-14808501 MS.gene08966:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 14808118 14808933 14808118 ID=MS.gene08966
chr4.4 mRNA 14808118 14808933 14808118 ID=MS.gene08966.t1;Parent=MS.gene08966
chr4.4 exon 14808118 14808933 14808118 ID=MS.gene08966.t1.exon1;Parent=MS.gene08966.t1
chr4.4 CDS 14808118 14808933 14808118 ID=cds.MS.gene08966.t1;Parent=MS.gene08966.t1
Gene Sequence

>MS.gene08966

ATGGGGGACCCACATTCAGCTTCAGATTTCATCAACTTATGGGGTCAAACCTTGAACAATTTGAGCCTAAAACTGCCCTTCAACATTCCAAGATCCGTCTCAACACCCCTTAATTTTGGATCCGAAAAGGAACCAGCTACATTTAAACGGGTTGACCCGGTTGGTGATCATTGGATCCCAGCCAACAACAAAAAAATGGACACATTCTCCTTCCACATAACTAGCTCCCTAATGAACAACTTGCAAGAACAAATTTGGGGTCCAAGTGTTGAACAAACCCCTCCATTTGAAACTTTATGTGCAATAATTTGGCATTGCATAGCCCGAGTTCGAGAAGGATCCGAACCCACAACCGTGACAGTTTGTCGACCCGACCCTAATGGTAGGGGCAATGATATAATGGGAAATGACCAATTGATATGCAAGGTTGAGGCAGGAAAAGAGTGTTCTATTGTTGACACTAGTTTGAAAATTTTGGCTAGCATGCTTGTGGATCAAGGTATTGATGAAAGAAATCAAATTATGGATATAGTAGAAAAAGATCAAGGGGTGACTGATTTTTTTGTCTATGGTGCCAATTTGACATTTCTTGACTTAGAAGATGTCAATGTGTATGACTTAAAATTAAAGGGACAAAAGCCAAGATTTGTTTACTACACACTTCAAGGGGTGGGAGATGAAGGTGTTGTTTTGGTGCTTCCTAAGGTGAATGATATTGATGGAAAATTTGTGACCATTATATTGCCAGAAGATGAAATGGAAAAACTTAAATCCGAGCTTAATGTGTATGGTATCATGTTTGAGGGTGATTTTTAA

Protein sequence

>MS.gene08966.t1

MGDPHSASDFINLWGQTLNNLSLKLPFNIPRSVSTPLNFGSEKEPATFKRVDPVGDHWIPANNKKMDTFSFHITSSLMNNLQEQIWGPSVEQTPPFETLCAIIWHCIARVREGSEPTTVTVCRPDPNGRGNDIMGNDQLICKVEAGKECSIVDTSLKILASMLVDQGIDERNQIMDIVEKDQGVTDFFVYGANLTFLDLEDVNVYDLKLKGQKPRFVYYTLQGVGDEGVVLVLPKVNDIDGKFVTIILPEDEMEKLKSELNVYGIMFEGDF