AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene20157


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene20157.t1 MTR_8g101900 96.596 235 8 0 1 235 1 235 7.65e-170 467
MS.gene20157.t1 MTR_8g021225 73.362 229 60 1 6 233 5 233 2.25e-121 345
MS.gene20157.t1 MTR_6g091680 70.996 231 67 0 5 235 8 238 1.26e-109 315
MS.gene20157.t1 MTR_4g085590 58.929 224 91 1 12 234 23 246 4.17e-96 281
MS.gene20157.t1 MTR_4g094925 54.626 227 102 1 9 234 16 242 1.12e-89 265
MS.gene20157.t1 MTR_4g094815 53.509 228 105 1 8 234 20 247 4.40e-89 263
MS.gene20157.t1 MTR_4g094898 54.825 228 102 1 8 234 20 247 3.02e-83 248
MS.gene20157.t1 MTR_4g094812 55.804 224 98 1 12 234 22 245 3.60e-80 241
MS.gene20157.t1 MTR_4g094368 52.232 224 103 3 12 232 5 227 1.52e-78 236
MS.gene20157.t1 MTR_4g094925 53.646 192 87 2 45 234 20 211 3.90e-74 224
MS.gene20157.t1 MTR_4g094898 55.914 186 81 1 50 234 1 186 3.24e-67 206
MS.gene20157.t1 MTR_2g070410 41.204 216 118 4 18 233 91 297 8.74e-49 162
MS.gene20157.t1 MTR_2g070410 41.860 172 96 2 18 189 91 258 3.81e-38 134
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene20157.t1 AT4G26220 64.574 223 79 0 12 234 9 231 8.52e-107 308
MS.gene20157.t1 AT4G34050 59.111 225 91 1 12 235 35 259 4.41e-96 281
MS.gene20157.t1 AT4G34050 59.111 225 91 1 12 235 62 286 5.48e-96 282
MS.gene20157.t1 AT1G67980 56.637 226 93 2 12 235 10 232 9.45e-88 259
MS.gene20157.t1 AT1G24735 55.947 227 95 3 12 235 16 240 1.74e-82 246
MS.gene20157.t1 AT1G67990 53.540 226 101 2 12 235 10 233 2.41e-80 241
MS.gene20157.t1 AT1G24735 45.848 277 94 4 12 234 16 290 4.35e-74 227
MS.gene20157.t1 AT1G24735 56.989 186 77 2 50 234 11 194 1.24e-70 214
MS.gene20157.t1 AT1G67980 50.909 165 78 1 70 234 2 163 9.72e-54 171
MS.gene20157.t1 AT3G61990 42.647 204 108 4 30 233 95 289 1.40e-48 161
MS.gene20157.t1 AT3G62000 40.000 205 115 4 30 233 80 277 8.75e-47 156
MS.gene20157.t1 AT4G34050 54.472 123 55 1 113 234 25 147 2.07e-44 146
MS.gene20157.t1 AT3G62000 37.383 214 123 5 11 223 64 267 7.07e-39 138

Find 39 sgRNAs with CRISPR-Local

Find 91 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTTCTGAAACTACCGTTTC+TGG 0.209949 4.4:+3816333 None:intergenic
CCATGAGAGGCTTATCAAAT+TGG 0.230744 4.4:-3816415 MS.gene20157:CDS
TCAAAAGTACCTTCATTTGC+AGG 0.353092 4.4:+3816480 None:intergenic
TGCCTACGATAACACCCTTT+GGG 0.369512 4.4:-3816373 MS.gene20157:CDS
GTAATTCTTCAGTGGCTTCA+TGG 0.374269 4.4:-3817254 MS.gene20157:intron
TCTAAAAGTTTATCAAGAAC+TGG 0.389451 4.4:+3816595 None:intergenic
ACCTGATGCGGGTCAGCTAA+TGG 0.395308 4.4:-3817224 MS.gene20157:CDS
ATACAAACCTTTCCATCATA+TGG 0.399941 4.4:+3817112 None:intergenic
TTGCCTACGATAACACCCTT+TGG 0.417902 4.4:-3816374 MS.gene20157:CDS
TTTCAGAAACAAAGAGAGAA+TGG 0.425659 4.4:-3816320 MS.gene20157:CDS
GACAGAAAAGCTTATGAGAT+AGG 0.427880 4.4:-3816682 MS.gene20157:CDS
TTATCAAGAACTGGCAAAGC+TGG 0.435178 4.4:+3816604 None:intergenic
GCCATTAGCTGACCCGCATC+AGG 0.436416 4.4:+3817223 None:intergenic
TAATTCTTCAGTGGCTTCAT+GGG 0.469413 4.4:-3817253 MS.gene20157:intron
ATCAAATTGGTTAAGATCGG+TGG 0.482512 4.4:-3816402 MS.gene20157:CDS
GCACTTAACATTCCATATGA+TGG 0.489413 4.4:-3817124 MS.gene20157:CDS
AACTGTTGCCTTGCCAGAAA+CGG 0.495235 4.4:-3816346 MS.gene20157:CDS
CTTATCAAATTGGTTAAGAT+CGG 0.511198 4.4:-3816405 MS.gene20157:CDS
TTGAGAGTCTCTGCTTCTCG+TGG 0.514299 4.4:+3817513 None:intergenic
CATGGGCACTTCACCTGATG+CGG 0.516416 4.4:-3817236 MS.gene20157:CDS
TGAAACTACCGTTTCTGGCA+AGG 0.521241 4.4:+3816338 None:intergenic
ATGGGCACTTCACCTGATGC+GGG 0.527395 4.4:-3817235 MS.gene20157:CDS
CTGCAAAATAACTGCATTGG+TGG 0.528279 4.4:+3817662 None:intergenic
CCAATTTGATAAGCCTCTCA+TGG 0.530119 4.4:+3816415 None:intergenic
TAACATTCCATATGATGGAA+AGG 0.530971 4.4:-3817119 MS.gene20157:intron
GCAGAGCGAGGGCTTACTCA+AGG 0.558409 4.4:-3817643 MS.gene20157:intron
TGCTTCTCGTGGGTAAACAC+TGG 0.559815 4.4:+3817524 None:intergenic
GGCAACAGTTCCTCCCCAAA+GGG 0.563275 4.4:+3816359 None:intergenic
AGGCAACAGTTCCTCCCCAA+AGG 0.565907 4.4:+3816358 None:intergenic
TCAAATTGGTTAAGATCGGT+GGG 0.567728 4.4:-3816401 MS.gene20157:CDS
TGAGAGTCTCTGCTTCTCGT+GGG 0.576132 4.4:+3817514 None:intergenic
GCTCTGCAAAATAACTGCAT+TGG 0.577755 4.4:+3817659 None:intergenic
GCGACGGACTCACTATCTGC+AGG 0.587918 4.4:-3816224 MS.gene20157:CDS
ACTATTGGAATTACCATGAG+AGG 0.588635 4.4:-3816428 MS.gene20157:CDS
TTGATGCAGCCTGCAAATGA+AGG 0.599302 4.4:-3816489 MS.gene20157:intron
GCCTACGATAACACCCTTTG+GGG 0.620350 4.4:-3816372 MS.gene20157:CDS
TACGATAACACCCTTTGGGG+AGG 0.624507 4.4:-3816369 MS.gene20157:CDS
TCCCCAAAGGGTGTTATCGT+AGG 0.628321 4.4:+3816371 None:intergenic
ACAAAAGCAAGTTCCATACG+AGG 0.723375 4.4:+3816255 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATTTTAGATTAATTAAGT+AGG + chr4.4:3816328-3816347 None:intergenic 10.0%
!!! TTAATTAATCTAAAATTTTG+TGG - chr4.4:3816330-3816349 MS.gene20157:CDS 10.0%
!! AAATAACTTCTATGATTTAT+AGG + chr4.4:3816475-3816494 None:intergenic 15.0%
!! AAAATTTGATTATGCATACA+AGG - chr4.4:3816503-3816522 MS.gene20157:intron 20.0%
!! ACAATGTTAAATGAATATGT+TGG - chr4.4:3817017-3817036 MS.gene20157:intron 20.0%
!!! AAAGTATCACATATAGTTTA+TGG - chr4.4:3816841-3816860 MS.gene20157:intron 20.0%
! AACTTCTATGATTTATAGGT+TGG + chr4.4:3816471-3816490 None:intergenic 25.0%
! AAGGAAAAGAAAAACCATTA+TGG + chr4.4:3817380-3817399 None:intergenic 25.0%
! AGGAAAAGAAAAACCATTAT+GGG + chr4.4:3817379-3817398 None:intergenic 25.0%
! AGTCTTAAGAATAATTACAG+AGG + chr4.4:3816434-3816453 None:intergenic 25.0%
! ATGCTGACAAAAATAACTAT+TGG - chr4.4:3817450-3817469 MS.gene20157:intron 25.0%
! ATGTTATTGTAATTCTTCAG+TGG - chr4.4:3816631-3816650 MS.gene20157:CDS 25.0%
! CTTATCAAATTGGTTAAGAT+CGG - chr4.4:3817488-3817507 MS.gene20157:CDS 25.0%
! TCTAAAAGTTTATCAAGAAC+TGG + chr4.4:3817301-3817320 None:intergenic 25.0%
!! TAGTTCAAAAGACTTTTACT+TGG + chr4.4:3816874-3816893 None:intergenic 25.0%
!!! TTTCAATGAAGCAGTTTTAA+AGG - chr4.4:3817613-3817632 MS.gene20157:intron 25.0%
AGGACTTCCATTCATAAAAA+AGG - chr4.4:3817231-3817250 MS.gene20157:CDS 30.0%
ATACAAACCTTTCCATCATA+TGG + chr4.4:3816784-3816803 None:intergenic 30.0%
CTAAACAGTTACAACAAACA+TGG + chr4.4:3816295-3816314 None:intergenic 30.0%
GCTAAAAAGACAATTGAAGT+AGG - chr4.4:3816715-3816734 MS.gene20157:intron 30.0%
TAAATGTATGCAAAGAGAGA+GGG + chr4.4:3817105-3817124 None:intergenic 30.0%
TTTCAGAAACAAAGAGAGAA+TGG - chr4.4:3817573-3817592 MS.gene20157:intron 30.0%
! ATTTTTGTCAGCATCAACAA+AGG + chr4.4:3817444-3817463 None:intergenic 30.0%
! CTTTTGAACTAGAACAATGA+TGG - chr4.4:3816883-3816902 MS.gene20157:intron 30.0%
! TAACATTCCATATGATGGAA+AGG - chr4.4:3816774-3816793 MS.gene20157:intron 30.0%
! TGATTTATAGGTTGGATGTA+TGG + chr4.4:3816463-3816482 None:intergenic 30.0%
!! GAACTTGCTTTTGTTTCAAT+AGG - chr4.4:3817647-3817666 MS.gene20157:CDS 30.0%
ACTATTGGAATTACCATGAG+AGG - chr4.4:3817465-3817484 MS.gene20157:intron 35.0%
ATCAAATTGGTTAAGATCGG+TGG - chr4.4:3817491-3817510 MS.gene20157:CDS 35.0%
ATCTCCACATATATCTTTCC+AGG - chr4.4:3817343-3817362 MS.gene20157:intron 35.0%
CTAAATGTATGCAAAGAGAG+AGG + chr4.4:3817106-3817125 None:intergenic 35.0%
CTTCCATTCATAAAAAAGGC+TGG - chr4.4:3817235-3817254 MS.gene20157:CDS 35.0%
GACAGAAAAGCTTATGAGAT+AGG - chr4.4:3817211-3817230 MS.gene20157:CDS 35.0%
GCACTTAACATTCCATATGA+TGG - chr4.4:3816769-3816788 MS.gene20157:intron 35.0%
TAATTCTTCAGTGGCTTCAT+GGG - chr4.4:3816640-3816659 MS.gene20157:CDS 35.0%
TCAAATTGGTTAAGATCGGT+GGG - chr4.4:3817492-3817511 MS.gene20157:CDS 35.0%
! ATCTCATAAGCTTTTCTGTC+TGG + chr4.4:3817211-3817230 None:intergenic 35.0%
! ATTGAAGTAGGAGTTTTCAC+TGG - chr4.4:3816727-3816746 MS.gene20157:intron 35.0%
!! TCAAAAGTACCTTCATTTGC+AGG + chr4.4:3817416-3817435 None:intergenic 35.0%
!!! ACACCAGCCTTTTTTATGAA+TGG + chr4.4:3817241-3817260 None:intergenic 35.0%
!!! AGTTTTAAAGGATCCTCGTA+TGG - chr4.4:3817625-3817644 MS.gene20157:intron 35.0%
AAACCATTATGGGTCTGACT+TGG + chr4.4:3817369-3817388 None:intergenic 40.0%
AATCACCGTCAATTTGGTAG+AGG - chr4.4:3816970-3816989 MS.gene20157:intron 40.0%
ACAAAAGCAAGTTCCATACG+AGG + chr4.4:3817641-3817660 None:intergenic 40.0%
CCAATTTGATAAGCCTCTCA+TGG + chr4.4:3817481-3817500 None:intergenic 40.0%
CCATGAGAGGCTTATCAAAT+TGG - chr4.4:3817478-3817497 MS.gene20157:intron 40.0%
CTGCAAAATAACTGCATTGG+TGG + chr4.4:3816234-3816253 None:intergenic 40.0%
CTGTAGAAGCCAGTATGTAT+AGG + chr4.4:3816817-3816836 None:intergenic 40.0%
CTTCTCCTCTACCAAATTGA+CGG + chr4.4:3816978-3816997 None:intergenic 40.0%
GAATCCAATCACCGTCAATT+TGG - chr4.4:3816964-3816983 MS.gene20157:intron 40.0%
GCTCTGCAAAATAACTGCAT+TGG + chr4.4:3816237-3816256 None:intergenic 40.0%
GTAATTCTTCAGTGGCTTCA+TGG - chr4.4:3816639-3816658 MS.gene20157:CDS 40.0%
TAGAAGCCAGTATGTATAGG+AGG + chr4.4:3816814-3816833 None:intergenic 40.0%
TCTACCAAATTGACGGTGAT+TGG + chr4.4:3816971-3816990 None:intergenic 40.0%
TGCTTTCCTCCTATACATAC+TGG - chr4.4:3816805-3816824 MS.gene20157:intron 40.0%
TTATCAAGAACTGGCAAAGC+TGG + chr4.4:3817292-3817311 None:intergenic 40.0%
TTGGCCTGGAAAGATATATG+TGG + chr4.4:3817350-3817369 None:intergenic 40.0%
! AGCTTTTCTGTCTGGATCTA+CGG + chr4.4:3817203-3817222 None:intergenic 40.0%
!! ATACATACTGGCTTCTACAG+TGG - chr4.4:3816817-3816836 MS.gene20157:intron 40.0%
!! GCTTTTGTTTCAATAGGCGA+CGG - chr4.4:3817653-3817672 MS.gene20157:CDS 40.0%
!! GTTTCTGAAACTACCGTTTC+TGG + chr4.4:3817563-3817582 None:intergenic 40.0%
AACTGTTGCCTTGCCAGAAA+CGG - chr4.4:3817547-3817566 MS.gene20157:intron 45.0%
ATCTACAGCAGGCAGACAAA+TGG + chr4.4:3817175-3817194 None:intergenic 45.0%
CGTCAATTTGGTAGAGGAGA+AGG - chr4.4:3816976-3816995 MS.gene20157:intron 45.0%
TCTACAGCAGGCAGACAAAT+GGG + chr4.4:3817174-3817193 None:intergenic 45.0%
TGACTTCTTCAGTCAGCATC+AGG - chr4.4:3816591-3816610 MS.gene20157:CDS 45.0%
TGCCTACGATAACACCCTTT+GGG - chr4.4:3817520-3817539 MS.gene20157:CDS 45.0%
TTGATGCAGCCTGCAAATGA+AGG - chr4.4:3817404-3817423 MS.gene20157:intron 45.0%
TTGCCTACGATAACACCCTT+TGG - chr4.4:3817519-3817538 MS.gene20157:CDS 45.0%
!! GCAGTTATTTTGCAGAGCGA+GGG - chr4.4:3816239-3816258 MS.gene20157:CDS 45.0%
!! TGAAACTACCGTTTCTGGCA+AGG + chr4.4:3817558-3817577 None:intergenic 45.0%
!! TGCAGTTATTTTGCAGAGCG+AGG - chr4.4:3816238-3816257 MS.gene20157:CDS 45.0%
AGGCCAAGTCAGACCCATAA+TGG - chr4.4:3817363-3817382 MS.gene20157:intron 50.0%
CTACAGCAGGCAGACAAATG+GGG + chr4.4:3817173-3817192 None:intergenic 50.0%
CTACGGCTGTAATCTACAGC+AGG + chr4.4:3817186-3817205 None:intergenic 50.0%
GCCTACGATAACACCCTTTG+GGG - chr4.4:3817521-3817540 MS.gene20157:CDS 50.0%
TGAGAGTCTCTGCTTCTCGT+GGG + chr4.4:3816382-3816401 None:intergenic 50.0%
TGCAGGCTGCATCAAGACAA+AGG + chr4.4:3817399-3817418 None:intergenic 50.0%
TGCTTCTCGTGGGTAAACAC+TGG + chr4.4:3816372-3816391 None:intergenic 50.0%
TTGAGAGTCTCTGCTTCTCG+TGG + chr4.4:3816383-3816402 None:intergenic 50.0%
! TACGATAACACCCTTTGGGG+AGG - chr4.4:3817524-3817543 MS.gene20157:CDS 50.0%
!! ATTATGGGTCTGACTTGGCC+TGG + chr4.4:3817364-3817383 None:intergenic 50.0%
!! TCCCCAAAGGGTGTTATCGT+AGG + chr4.4:3817525-3817544 None:intergenic 50.0%
ACCTGATGCGGGTCAGCTAA+TGG - chr4.4:3816669-3816688 MS.gene20157:CDS 55.0%
AGGCAACAGTTCCTCCCCAA+AGG + chr4.4:3817538-3817557 None:intergenic 55.0%
ATGGGCACTTCACCTGATGC+GGG - chr4.4:3816658-3816677 MS.gene20157:CDS 55.0%
CATGGGCACTTCACCTGATG+CGG - chr4.4:3816657-3816676 MS.gene20157:CDS 55.0%
GGCAACAGTTCCTCCCCAAA+GGG + chr4.4:3817537-3817556 None:intergenic 55.0%
GCAGAGCGAGGGCTTACTCA+AGG - chr4.4:3816250-3816269 MS.gene20157:CDS 60.0%
GCCATTAGCTGACCCGCATC+AGG + chr4.4:3816673-3816692 None:intergenic 60.0%
! GCGACGGACTCACTATCTGC+AGG - chr4.4:3817669-3817688 MS.gene20157:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 3816212 3817703 3816212 ID=MS.gene20157
chr4.4 mRNA 3816212 3817703 3816212 ID=MS.gene20157.t1;Parent=MS.gene20157
chr4.4 exon 3817644 3817703 3817644 ID=MS.gene20157.t1.exon1;Parent=MS.gene20157.t1
chr4.4 CDS 3817644 3817703 3817644 ID=cds.MS.gene20157.t1;Parent=MS.gene20157.t1
chr4.4 exon 3817480 3817559 3817480 ID=MS.gene20157.t1.exon2;Parent=MS.gene20157.t1
chr4.4 CDS 3817480 3817559 3817480 ID=cds.MS.gene20157.t1;Parent=MS.gene20157.t1
chr4.4 exon 3817120 3817264 3817120 ID=MS.gene20157.t1.exon3;Parent=MS.gene20157.t1
chr4.4 CDS 3817120 3817264 3817120 ID=cds.MS.gene20157.t1;Parent=MS.gene20157.t1
chr4.4 exon 3816591 3816722 3816591 ID=MS.gene20157.t1.exon4;Parent=MS.gene20157.t1
chr4.4 CDS 3816591 3816722 3816591 ID=cds.MS.gene20157.t1;Parent=MS.gene20157.t1
chr4.4 exon 3816212 3816502 3816212 ID=MS.gene20157.t1.exon5;Parent=MS.gene20157.t1
chr4.4 CDS 3816212 3816502 3816212 ID=cds.MS.gene20157.t1;Parent=MS.gene20157.t1
Gene Sequence

>MS.gene20157

ATGGACAAAATATCAGCTTCCACCAATGCAGTTATTTTGCAGAGCGAGGGCTTACTCAAGGTTTACATACACATCTCTTGCCATGTTTGTTGTAACTGTTTAGATTGATTTTTCCTACTTAATTAATCTAAAATTTTGTGGCAGTATATACTAGAAACCAGTGTTTACCCACGAGAAGCAGAGACTCTCAAAGAGCTGAGAAATGCAACAGCAAATCATCCTCTGTAATTATTCTTAAGACTGCTATACCATACATCCAACCTATAAATCATAGAAGTTATTTAACATAACAAAATTTGATTATGCATACAAGGATTTTTCAAACAAAAATAGTATGATATTATTATTATTATTATTATTATTTTATTCTTGTTGAACTTGACTTCTTCAGTCAGCATCAGGAAACTTTTTCTTATGTAATGTTATTGTAATTCTTCAGTGGCTTCATGGGCACTTCACCTGATGCGGGTCAGCTAATGGCTTTGCTCTTGAAACTTCTAAATGCTAAAAAGACAATTGAAGTAGGAGTTTTCACTGGATACTCTCTTCTACTCACAGCACTTAACATTCCATATGATGGAAAGGTTTGTATCTGCTTTCCTCCTATACATACTGGCTTCTACAGTGGAAAAGTATCACATATAGTTTATGGTAGATCACCAAGTAAAAGTCTTTTGAACTAGAACAATGATGGATGTTGCTCTTCGTAAGTAGAAGCTCATGATATTATGCATTCTCAAGACTGAGACGATGAATCCAATCACCGTCAATTTGGTAGAGGAGAAGGAACAAAAAAACTATTTGAACAATGTTAAATGAATATGTTGGTCATCGTAAGAGTTTTGAACAATTTCAATGCATAAGATGCTTAATTCTCACGCTAGCAATCACCCTCTCTCTTTGCATACATTTAGATAATAACGTCGCAAGTATGTGTCTATTATTTTTATGTTACTAGCCCCATTTGTCTGCCTGCTGTAGATTACAGCCGTAGATCCAGACAGAAAAGCTTATGAGATAGGACTTCCATTCATAAAAAAGGCTGGTGTCTTACACAAGATTGACTACATAGAGTCTCCAGCTTTGCCAGTTCTTGATAAACTTTTAGAAGATGTAAGCAACTTACTTAACATCTCCACATATATCTTTCCAGGCCAAGTCAGACCCATAATGGTTTTTCTTTTCCTTTGTCTTGATGCAGCCTGCAAATGAAGGTACTTTTGACTTTGCCTTTGTTGATGCTGACAAAAATAACTATTGGAATTACCATGAGAGGCTTATCAAATTGGTTAAGATCGGTGGGATTGTTGCCTACGATAACACCCTTTGGGGAGGAACTGTTGCCTTGCCAGAAACGGTAGTTTCAGAAACAAAGAGAGAATGGAGAAGATGTACACTTGATTTCAATGAAGCAGTTTTAAAGGATCCTCGTATGGAACTTGCTTTTGTTTCAATAGGCGACGGACTCACTATCTGCAGGCGCATTTGCTGA

Protein sequence

>MS.gene20157.t1

MDKISASTNAVILQSEGLLKYILETSVYPREAETLKELRNATANHPLGFMGTSPDAGQLMALLLKLLNAKKTIEVGVFTGYSLLLTALNIPYDGKITAVDPDRKAYEIGLPFIKKAGVLHKIDYIESPALPVLDKLLEDPANEGTFDFAFVDADKNNYWNYHERLIKLVKIGGIVAYDNTLWGGTVALPETVVSETKREWRRCTLDFNEAVLKDPRMELAFVSIGDGLTICRRIC