Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21122.t1 | XP_013469134.1 | 81.7 | 388 | 14 | 1 | 1 | 331 | 1 | 388 | 2.80E-173 | 617.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21122.t1 | Q8VZQ2 | 28.8 | 417 | 155 | 12 | 1 | 329 | 1 | 363 | 2.3e-30 | 134.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21122.t1 | A0A072VMV6 | 81.7 | 388 | 14 | 1 | 1 | 331 | 1 | 388 | 2.0e-173 | 617.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene21122.t1 | TF | MYB-related |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049770 | MS.gene21122 | 0.801239 | 9.89E-49 | -1.69E-46 |
| MS.gene05045 | MS.gene21122 | 0.80594 | 1.05E-49 | -1.69E-46 |
| MS.gene050807 | MS.gene21122 | 0.835369 | 1.75E-56 | -1.69E-46 |
| MS.gene050808 | MS.gene21122 | 0.822773 | 1.98E-53 | -1.69E-46 |
| MS.gene051247 | MS.gene21122 | 0.811736 | 6.03E-51 | -1.69E-46 |
| MS.gene05157 | MS.gene21122 | 0.828424 | 9.06E-55 | -1.69E-46 |
| MS.gene051719 | MS.gene21122 | 0.813697 | 2.24E-51 | -1.69E-46 |
| MS.gene051720 | MS.gene21122 | 0.844172 | 8.91E-59 | -1.69E-46 |
| MS.gene051744 | MS.gene21122 | 0.806423 | 8.29E-50 | -1.69E-46 |
| MS.gene052020 | MS.gene21122 | 0.819259 | 1.28E-52 | -1.69E-46 |
| MS.gene052133 | MS.gene21122 | 0.802796 | 4.73E-49 | -1.69E-46 |
| MS.gene052665 | MS.gene21122 | 0.817356 | 3.44E-52 | -1.69E-46 |
| MS.gene052732 | MS.gene21122 | 0.820114 | 8.14E-53 | -1.69E-46 |
| MS.gene053144 | MS.gene21122 | 0.819593 | 1.07E-52 | -1.69E-46 |
| MS.gene053581 | MS.gene21122 | 0.805334 | 1.40E-49 | -1.69E-46 |
| MS.gene054584 | MS.gene21122 | 0.816978 | 4.19E-52 | -1.69E-46 |
| MS.gene055144 | MS.gene21122 | 0.80043 | 1.45E-48 | -1.69E-46 |
| MS.gene055465 | MS.gene21122 | 0.821019 | 5.04E-53 | -1.69E-46 |
| MS.gene055466 | MS.gene21122 | 0.819901 | 9.10E-53 | -1.69E-46 |
| MS.gene055468 | MS.gene21122 | 0.803201 | 3.90E-49 | -1.69E-46 |
| MS.gene055537 | MS.gene21122 | 0.81084 | 9.44E-51 | -1.69E-46 |
| MS.gene056553 | MS.gene21122 | 0.809777 | 1.60E-50 | -1.69E-46 |
| MS.gene058168 | MS.gene21122 | 0.815066 | 1.12E-51 | -1.69E-46 |
| MS.gene060429 | MS.gene21122 | 0.804264 | 2.35E-49 | -1.69E-46 |
| MS.gene060519 | MS.gene21122 | 0.827179 | 1.80E-54 | -1.69E-46 |
| MS.gene061176 | MS.gene21122 | 0.800498 | 1.40E-48 | -1.69E-46 |
| MS.gene061178 | MS.gene21122 | 0.800007 | 1.76E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21122.t1 | MTR_1g085640 | 80.412 | 388 | 19 | 2 | 1 | 331 | 1 | 388 | 0.0 | 619 |
| MS.gene21122.t1 | MTR_7g110830 | 46.565 | 393 | 94 | 18 | 1 | 312 | 1 | 358 | 2.67e-84 | 259 |
| MS.gene21122.t1 | MTR_1g085040 | 32.010 | 403 | 163 | 16 | 1 | 329 | 1 | 366 | 2.59e-29 | 115 |
| MS.gene21122.t1 | MTR_4g105130 | 42.748 | 131 | 18 | 3 | 1 | 74 | 1 | 131 | 1.50e-21 | 94.4 |
| MS.gene21122.t1 | MTR_3g077110 | 48.276 | 87 | 38 | 2 | 12 | 94 | 65 | 148 | 3.99e-17 | 81.6 |
| MS.gene21122.t1 | MTR_1g112760 | 55.385 | 65 | 25 | 1 | 12 | 72 | 66 | 130 | 4.99e-17 | 79.3 |
| MS.gene21122.t1 | MTR_7g076740 | 52.174 | 69 | 27 | 2 | 12 | 75 | 66 | 133 | 1.13e-15 | 76.6 |
| MS.gene21122.t1 | MTR_7g111290 | 42.446 | 139 | 23 | 3 | 1 | 82 | 1 | 139 | 1.58e-15 | 77.0 |
| MS.gene21122.t1 | MTR_4g073420 | 50.000 | 68 | 30 | 1 | 12 | 75 | 65 | 132 | 1.82e-15 | 75.9 |
| MS.gene21122.t1 | MTR_2g064160 | 34.615 | 130 | 27 | 3 | 1 | 73 | 1 | 129 | 2.47e-15 | 75.9 |
| MS.gene21122.t1 | MTR_2g089620 | 52.239 | 67 | 28 | 1 | 9 | 71 | 62 | 128 | 3.17e-15 | 75.9 |
| MS.gene21122.t1 | MTR_8g031360 | 55.224 | 67 | 24 | 2 | 12 | 73 | 67 | 132 | 4.08e-15 | 75.1 |
| MS.gene21122.t1 | MTR_6g009430 | 31.818 | 132 | 33 | 2 | 1 | 75 | 1 | 132 | 7.27e-15 | 74.7 |
| MS.gene21122.t1 | MTR_4g091490 | 53.125 | 64 | 26 | 1 | 12 | 71 | 66 | 129 | 7.55e-15 | 74.7 |
| MS.gene21122.t1 | MTR_2g011660 | 53.125 | 64 | 26 | 1 | 12 | 71 | 66 | 129 | 8.29e-15 | 74.7 |
| MS.gene21122.t1 | MTR_8g095390 | 52.941 | 68 | 28 | 2 | 12 | 75 | 65 | 132 | 9.55e-15 | 74.3 |
| MS.gene21122.t1 | MTR_3g045430 | 59.016 | 61 | 22 | 1 | 18 | 75 | 73 | 133 | 1.15e-14 | 73.9 |
| MS.gene21122.t1 | MTR_5g007370 | 50.746 | 67 | 29 | 1 | 12 | 74 | 65 | 131 | 2.00e-14 | 73.2 |
| MS.gene21122.t1 | MTR_4g478180 | 53.125 | 64 | 26 | 2 | 12 | 71 | 66 | 129 | 2.06e-14 | 73.6 |
| MS.gene21122.t1 | MTR_3g074520 | 50.725 | 69 | 28 | 2 | 12 | 75 | 65 | 132 | 3.00e-14 | 73.2 |
| MS.gene21122.t1 | MTR_5g078140 | 53.731 | 67 | 25 | 2 | 12 | 73 | 65 | 130 | 3.11e-14 | 72.8 |
| MS.gene21122.t1 | MTR_3g461490 | 51.471 | 68 | 29 | 1 | 12 | 75 | 65 | 132 | 3.62e-14 | 72.8 |
| MS.gene21122.t1 | MTR_7g111290 | 56.410 | 78 | 30 | 2 | 9 | 82 | 20 | 97 | 7.52e-14 | 71.6 |
| MS.gene21122.t1 | MTR_7g011170 | 52.239 | 67 | 28 | 2 | 12 | 74 | 65 | 131 | 1.02e-13 | 71.2 |
| MS.gene21122.t1 | MTR_3g065440 | 55.224 | 67 | 24 | 2 | 12 | 73 | 65 | 130 | 1.02e-13 | 71.2 |
| MS.gene21122.t1 | MTR_3g083540 | 50.000 | 68 | 30 | 2 | 9 | 72 | 91 | 158 | 1.11e-13 | 70.9 |
| MS.gene21122.t1 | MTR_4g102380 | 49.254 | 67 | 30 | 1 | 12 | 74 | 65 | 131 | 1.41e-13 | 70.1 |
| MS.gene21122.t1 | MTR_4g082230 | 54.688 | 64 | 25 | 2 | 12 | 71 | 65 | 128 | 1.65e-13 | 71.2 |
| MS.gene21122.t1 | MTR_4g082290 | 54.688 | 64 | 25 | 2 | 12 | 71 | 65 | 128 | 1.71e-13 | 71.2 |
| MS.gene21122.t1 | MTR_5g029840 | 47.761 | 67 | 31 | 1 | 12 | 74 | 65 | 131 | 3.51e-13 | 69.7 |
| MS.gene21122.t1 | MTR_5g042030 | 56.061 | 66 | 25 | 2 | 12 | 73 | 60 | 125 | 4.63e-13 | 68.9 |
| MS.gene21122.t1 | MTR_4g065017 | 54.386 | 57 | 20 | 2 | 9 | 60 | 67 | 122 | 1.12e-12 | 68.2 |
| MS.gene21122.t1 | MTR_3g097450 | 46.269 | 67 | 32 | 1 | 12 | 74 | 65 | 131 | 1.60e-12 | 67.4 |
| MS.gene21122.t1 | MTR_6g012690 | 51.562 | 64 | 27 | 2 | 12 | 71 | 65 | 128 | 1.94e-12 | 67.8 |
| MS.gene21122.t1 | MTR_3g028740 | 55.357 | 56 | 21 | 1 | 9 | 60 | 72 | 127 | 4.23e-12 | 66.6 |
| MS.gene21122.t1 | MTR_8g098860 | 32.203 | 118 | 23 | 2 | 1 | 61 | 1 | 118 | 1.36e-11 | 65.1 |
| MS.gene21122.t1 | MTR_2g097910 | 54.717 | 53 | 20 | 1 | 12 | 60 | 73 | 125 | 3.40e-11 | 63.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21122.t1 | AT4G01680 | 48.026 | 152 | 21 | 3 | 1 | 95 | 1 | 151 | 3.83e-33 | 125 |
| MS.gene21122.t1 | AT1G09540 | 26.555 | 418 | 163 | 14 | 1 | 329 | 1 | 363 | 4.51e-27 | 109 |
| MS.gene21122.t1 | AT4G01680 | 42.105 | 152 | 30 | 4 | 1 | 95 | 1 | 151 | 5.83e-25 | 101 |
| MS.gene21122.t1 | AT1G57560 | 32.558 | 215 | 82 | 6 | 1 | 152 | 1 | 215 | 1.51e-23 | 99.0 |
| MS.gene21122.t1 | AT4G01680 | 56.818 | 88 | 33 | 3 | 12 | 95 | 77 | 163 | 4.62e-22 | 95.5 |
| MS.gene21122.t1 | AT4G01680 | 76.744 | 43 | 5 | 1 | 1 | 38 | 1 | 43 | 3.56e-14 | 72.8 |
| MS.gene21122.t1 | AT1G57560 | 42.105 | 133 | 20 | 3 | 1 | 76 | 1 | 133 | 5.62e-22 | 94.0 |
| MS.gene21122.t1 | AT5G26660 | 41.860 | 129 | 18 | 3 | 1 | 72 | 1 | 129 | 9.38e-22 | 94.7 |
| MS.gene21122.t1 | AT5G26660 | 61.538 | 65 | 21 | 2 | 12 | 72 | 34 | 98 | 2.58e-19 | 87.4 |
| MS.gene21122.t1 | AT5G60890 | 52.703 | 74 | 31 | 2 | 12 | 81 | 65 | 138 | 1.42e-17 | 82.0 |
| MS.gene21122.t1 | AT5G16600 | 52.564 | 78 | 33 | 1 | 12 | 85 | 65 | 142 | 2.23e-17 | 82.0 |
| MS.gene21122.t1 | AT1G66230 | 51.250 | 80 | 35 | 1 | 12 | 87 | 65 | 144 | 1.08e-16 | 79.3 |
| MS.gene21122.t1 | AT1G74430 | 51.562 | 64 | 27 | 1 | 12 | 71 | 65 | 128 | 1.60e-16 | 78.6 |
| MS.gene21122.t1 | AT5G10280 | 48.052 | 77 | 36 | 1 | 3 | 75 | 56 | 132 | 2.82e-16 | 79.0 |
| MS.gene21122.t1 | AT1G74430 | 72.093 | 43 | 12 | 0 | 29 | 71 | 17 | 59 | 4.30e-16 | 76.3 |
| MS.gene21122.t1 | AT5G61420 | 53.425 | 73 | 30 | 2 | 9 | 77 | 62 | 134 | 8.06e-16 | 77.8 |
| MS.gene21122.t1 | AT4G28110 | 47.619 | 84 | 30 | 2 | 2 | 71 | 45 | 128 | 2.48e-15 | 75.5 |
| MS.gene21122.t1 | AT3G61250 | 36.434 | 129 | 25 | 2 | 1 | 72 | 1 | 129 | 2.75e-15 | 75.5 |
| MS.gene21122.t1 | AT4G05100 | 54.688 | 64 | 25 | 1 | 12 | 71 | 66 | 129 | 3.46e-15 | 75.5 |
| MS.gene21122.t1 | AT4G21440 | 53.125 | 64 | 26 | 1 | 12 | 71 | 65 | 128 | 3.85e-15 | 75.9 |
| MS.gene21122.t1 | AT5G61420 | 70.213 | 47 | 14 | 0 | 31 | 77 | 9 | 55 | 4.38e-15 | 74.7 |
| MS.gene21122.t1 | AT5G07700 | 41.525 | 118 | 50 | 4 | 9 | 107 | 62 | 179 | 7.03e-15 | 74.7 |
| MS.gene21122.t1 | AT5G07700 | 41.525 | 118 | 50 | 4 | 9 | 107 | 62 | 179 | 7.03e-15 | 74.7 |
| MS.gene21122.t1 | AT5G65230 | 48.684 | 76 | 35 | 2 | 3 | 74 | 56 | 131 | 9.47e-15 | 74.3 |
| MS.gene21122.t1 | AT1G18710 | 48.529 | 68 | 31 | 2 | 9 | 72 | 62 | 129 | 1.04e-14 | 73.6 |
| MS.gene21122.t1 | AT1G18570 | 51.515 | 66 | 28 | 1 | 12 | 73 | 66 | 131 | 1.28e-14 | 74.3 |
| MS.gene21122.t1 | AT4G28110 | 70.732 | 41 | 12 | 0 | 31 | 71 | 4 | 44 | 1.47e-14 | 71.6 |
| MS.gene21122.t1 | AT4G34990 | 50.746 | 67 | 29 | 1 | 12 | 74 | 65 | 131 | 2.19e-14 | 72.8 |
| MS.gene21122.t1 | AT3G12720 | 33.333 | 126 | 26 | 3 | 1 | 69 | 12 | 136 | 2.23e-14 | 73.2 |
| MS.gene21122.t1 | AT5G07690 | 51.282 | 78 | 34 | 2 | 9 | 82 | 62 | 139 | 2.32e-14 | 73.2 |
| MS.gene21122.t1 | AT1G34670 | 50.000 | 72 | 30 | 2 | 9 | 75 | 62 | 132 | 2.47e-14 | 73.6 |
| MS.gene21122.t1 | AT3G13540 | 57.143 | 63 | 24 | 1 | 13 | 72 | 78 | 140 | 2.80e-14 | 72.0 |
| MS.gene21122.t1 | AT5G15310 | 64.000 | 50 | 18 | 0 | 25 | 74 | 3 | 52 | 2.83e-14 | 72.0 |
| MS.gene21122.t1 | AT4G17785 | 52.239 | 67 | 28 | 2 | 9 | 71 | 63 | 129 | 3.11e-14 | 73.2 |
| MS.gene21122.t1 | AT4G17785 | 52.239 | 67 | 28 | 2 | 9 | 71 | 63 | 129 | 3.36e-14 | 73.2 |
| MS.gene21122.t1 | AT5G16770 | 49.254 | 67 | 30 | 1 | 12 | 74 | 65 | 131 | 4.81e-14 | 72.4 |
| MS.gene21122.t1 | AT5G16770 | 49.254 | 67 | 30 | 1 | 12 | 74 | 65 | 131 | 4.81e-14 | 72.4 |
| MS.gene21122.t1 | AT5G56110 | 68.889 | 45 | 14 | 0 | 31 | 75 | 88 | 132 | 5.96e-14 | 72.0 |
| MS.gene21122.t1 | AT4G38620 | 47.761 | 67 | 31 | 1 | 12 | 74 | 65 | 131 | 6.50e-14 | 71.2 |
| MS.gene21122.t1 | AT5G16770 | 49.254 | 67 | 30 | 1 | 12 | 74 | 65 | 131 | 7.03e-14 | 72.0 |
| MS.gene21122.t1 | AT5G16770 | 49.254 | 67 | 30 | 1 | 12 | 74 | 65 | 131 | 7.03e-14 | 72.0 |
| MS.gene21122.t1 | AT4G22680 | 52.239 | 67 | 28 | 2 | 12 | 74 | 65 | 131 | 7.30e-14 | 70.9 |
| MS.gene21122.t1 | AT2G16720 | 50.746 | 67 | 29 | 1 | 12 | 74 | 65 | 131 | 7.78e-14 | 70.9 |
| MS.gene21122.t1 | AT3G02940 | 47.761 | 67 | 31 | 1 | 12 | 74 | 65 | 131 | 8.02e-14 | 71.6 |
| MS.gene21122.t1 | AT3G02940 | 47.761 | 67 | 31 | 1 | 12 | 74 | 65 | 131 | 1.14e-13 | 71.2 |
| MS.gene21122.t1 | AT3G02940 | 47.761 | 67 | 31 | 1 | 12 | 74 | 65 | 131 | 1.14e-13 | 71.2 |
| MS.gene21122.t1 | AT5G14340 | 39.759 | 83 | 47 | 1 | 31 | 113 | 10 | 89 | 1.57e-13 | 68.6 |
| MS.gene21122.t1 | AT1G22640 | 47.761 | 67 | 31 | 1 | 12 | 74 | 8 | 74 | 2.18e-13 | 68.6 |
| MS.gene21122.t1 | AT4G09460 | 38.318 | 107 | 62 | 1 | 12 | 114 | 65 | 171 | 2.62e-13 | 68.9 |
| MS.gene21122.t1 | AT5G14340 | 37.500 | 104 | 59 | 2 | 13 | 113 | 79 | 179 | 4.38e-13 | 68.9 |
| MS.gene21122.t1 | AT5G54230 | 46.575 | 73 | 35 | 1 | 3 | 71 | 56 | 128 | 4.43e-13 | 69.3 |
| MS.gene21122.t1 | AT5G15310 | 70.455 | 44 | 13 | 0 | 31 | 74 | 88 | 131 | 7.01e-13 | 68.9 |
| MS.gene21122.t1 | AT5G15310 | 70.455 | 44 | 13 | 0 | 31 | 74 | 88 | 131 | 7.43e-13 | 68.9 |
| MS.gene21122.t1 | AT5G15310 | 70.455 | 44 | 13 | 0 | 31 | 74 | 88 | 131 | 7.91e-13 | 67.8 |
| MS.gene21122.t1 | AT4G12350 | 50.000 | 68 | 30 | 2 | 12 | 75 | 65 | 132 | 8.16e-13 | 68.2 |
| MS.gene21122.t1 | AT1G22640 | 47.761 | 67 | 31 | 1 | 12 | 74 | 65 | 131 | 1.67e-12 | 67.0 |
| MS.gene21122.t1 | AT5G12870 | 55.556 | 54 | 20 | 1 | 12 | 61 | 71 | 124 | 1.76e-12 | 67.4 |
| MS.gene21122.t1 | AT1G74080 | 49.231 | 65 | 29 | 1 | 12 | 72 | 65 | 129 | 3.45e-12 | 67.0 |
| MS.gene21122.t1 | AT5G62320 | 44.595 | 74 | 37 | 1 | 12 | 81 | 73 | 146 | 3.54e-12 | 65.9 |
| MS.gene21122.t1 | AT1G63910 | 31.250 | 128 | 31 | 2 | 1 | 71 | 1 | 128 | 6.42e-12 | 66.2 |
| MS.gene21122.t1 | AT3G01140 | 50.794 | 63 | 27 | 2 | 12 | 70 | 65 | 127 | 8.70e-12 | 65.9 |
| MS.gene21122.t1 | AT3G01140 | 50.794 | 63 | 27 | 2 | 12 | 70 | 108 | 170 | 9.45e-12 | 65.9 |
| MS.gene21122.t1 | AT1G22640 | 50.000 | 54 | 27 | 0 | 21 | 74 | 6 | 59 | 1.90e-11 | 62.8 |
| MS.gene21122.t1 | AT1G35515 | 61.364 | 44 | 17 | 0 | 31 | 74 | 88 | 131 | 2.95e-11 | 62.8 |
| MS.gene21122.t1 | AT3G08500 | 56.140 | 57 | 19 | 2 | 9 | 60 | 80 | 135 | 3.07e-11 | 63.9 |
| MS.gene21122.t1 | AT3G28470 | 44.444 | 90 | 43 | 3 | 9 | 91 | 62 | 151 | 5.20e-11 | 63.2 |
Find 53 sgRNAs with CRISPR-Local
Find 89 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACAATGAAATAAAGAATTTA+TGG | 0.200655 | 1.4:-67786005 | MS.gene21122:CDS |
| TTCTTCTTGGGGAGGATTAA+TGG | 0.211991 | 1.4:-67785404 | MS.gene21122:CDS |
| ATTGTTGTTATGTAAGTTTA+AGG | 0.238149 | 1.4:+67785429 | None:intergenic |
| ATTTCCATTGCATATGCTTT+TGG | 0.239502 | 1.4:+67785763 | None:intergenic |
| TTTCAAAAGGTTCAATTCAT+TGG | 0.290203 | 1.4:+67785849 | None:intergenic |
| CAGAAACTCAGAGAAGCTTT+TGG | 0.294526 | 1.4:-67785172 | MS.gene21122:CDS |
| GAGAATAATGGAGAGGAAAA+TGG | 0.306414 | 1.4:-67785910 | MS.gene21122:CDS |
| CATTCAACCCTCAGATTTGA+TGG | 0.319312 | 1.4:-67785701 | MS.gene21122:CDS |
| AACTTTGTACCAGATAGTTT+TGG | 0.328603 | 1.4:-67785268 | MS.gene21122:CDS |
| CTACTAGTGTTTGAATCATT+TGG | 0.336320 | 1.4:+67785628 | None:intergenic |
| TCAGAAGCAAGCATTAAGAT+AGG | 0.337065 | 1.4:+67785325 | None:intergenic |
| TCAAATTGCGGCGCAATTGC+CGG | 0.343894 | 1.4:-67786037 | MS.gene21122:CDS |
| AAGAAGAGACTGAGACAAAA+AGG | 0.366324 | 1.4:-67785970 | MS.gene21122:CDS |
| TATCAAAAGATTTAACAGTT+TGG | 0.369030 | 1.4:+67785590 | None:intergenic |
| AAAATAGCTCCAAAACTATC+TGG | 0.372468 | 1.4:+67785259 | None:intergenic |
| CAAATTGCGGCGCAATTGCC+GGG | 0.376424 | 1.4:-67786036 | MS.gene21122:CDS |
| AAAAGCATATGCAATGGAAA+TGG | 0.406668 | 1.4:-67785761 | MS.gene21122:CDS |
| TCTGAGTTTCTGAATGTCCT+TGG | 0.406727 | 1.4:+67785183 | None:intergenic |
| TTGTCTGAGATTGAGAATAA+TGG | 0.407472 | 1.4:-67785922 | MS.gene21122:CDS |
| TCTTGTTCTTGCTTTGTATG+AGG | 0.413126 | 1.4:+67785235 | None:intergenic |
| TTGAACTCTCTGATTTCAAA+AGG | 0.423755 | 1.4:+67785836 | None:intergenic |
| GTTGTTACAAGCAGAAGCTT+AGG | 0.455012 | 1.4:-67786559 | MS.gene21122:CDS |
| CATGGTTGTTGATGTTGTTG+TGG | 0.467386 | 1.4:+67785540 | None:intergenic |
| CATCTCTGTGGAAACATTGT+CGG | 0.472691 | 1.4:+67785471 | None:intergenic |
| TCATTGGATAATAATATCTC+TGG | 0.476615 | 1.4:+67785865 | None:intergenic |
| ATGGTTGTTGATGTTGTTGT+GGG | 0.482724 | 1.4:+67785541 | None:intergenic |
| TTTGTATAACTCATTTGAAG+TGG | 0.483418 | 1.4:+67785670 | None:intergenic |
| TAACAGTTTGGTTGAACCAA+TGG | 0.500655 | 1.4:+67785602 | None:intergenic |
| TTATTCTCAATCTCAGACAA+TGG | 0.501529 | 1.4:+67785925 | None:intergenic |
| CAGAAGCAAGCATTAAGATA+GGG | 0.504178 | 1.4:+67785326 | None:intergenic |
| TCTGGTACAAAGTTCATCAA+TGG | 0.513100 | 1.4:+67785277 | None:intergenic |
| ATTTCATTGTCTGTCCTCCC+CGG | 0.513376 | 1.4:+67786018 | None:intergenic |
| GACAATGGTTTATGAGTCAC+AGG | 0.514909 | 1.4:+67785940 | None:intergenic |
| AGAAGCTTAGGAAAGGTCTA+TGG | 0.519654 | 1.4:-67786547 | MS.gene21122:CDS |
| AAAATTTCCCATCAAATCTG+AGG | 0.537811 | 1.4:+67785693 | None:intergenic |
| ATTCAACCCTCAGATTTGAT+GGG | 0.538604 | 1.4:-67785700 | MS.gene21122:CDS |
| TCATACAGAAGAAGCTAAGT+GGG | 0.564291 | 1.4:-67785377 | MS.gene21122:CDS |
| CTCTGTGGAAACATTGTCGG+AGG | 0.573457 | 1.4:+67785474 | None:intergenic |
| TTCACCAAAAGCATATGCAA+TGG | 0.574736 | 1.4:-67785767 | MS.gene21122:CDS |
| ATCATACAGAAGAAGCTAAG+TGG | 0.575317 | 1.4:-67785378 | MS.gene21122:CDS |
| TATCAGATGGTCTCAAATTG+CGG | 0.579070 | 1.4:-67786049 | MS.gene21122:intron |
| AGGAAAATTCACAAATGGGA+AGG | 0.581895 | 1.4:-67786589 | None:intergenic |
| TTGCGGCGCAATTGCCGGGG+AGG | 0.583739 | 1.4:-67786032 | MS.gene21122:CDS |
| AAATTTCCCATCAAATCTGA+GGG | 0.588053 | 1.4:+67785694 | None:intergenic |
| CAAACACTAGTAGTAACCAT+TGG | 0.594685 | 1.4:-67785618 | MS.gene21122:CDS |
| GCATATGCAATGGAAATGGA+AGG | 0.607612 | 1.4:-67785757 | MS.gene21122:CDS |
| TACAAGCAGAAGCTTAGGAA+AGG | 0.614815 | 1.4:-67786554 | MS.gene21122:CDS |
| GGTACAAAGTTCATCAATGG+AGG | 0.632864 | 1.4:+67785280 | None:intergenic |
| GCTGCTAGTTCTCATCTCTG+TGG | 0.644097 | 1.4:+67785459 | None:intergenic |
| AAATTGCGGCGCAATTGCCG+GGG | 0.649843 | 1.4:-67786035 | MS.gene21122:CDS |
| TGAGATTGAGAATAATGGAG+AGG | 0.653412 | 1.4:-67785917 | MS.gene21122:CDS |
| TCTGTGGAAACATTGTCGGA+GGG | 0.657513 | 1.4:+67785475 | None:intergenic |
| AAAAGAATTAGGAAGAAACA+TGG | 0.684494 | 1.4:+67785522 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTATTATTATTATATGCTAA+TGG | + | chr1.4:67785332-67785351 | None:intergenic | 10.0% |
| !!! | AATTTTTGTTTATTATTATC+AGG | - | chr1.4:67785386-67785405 | MS.gene21122:CDS | 10.0% |
| !!! | ATGAACATAATTTTTTAAAA+CGG | + | chr1.4:67785660-67785679 | None:intergenic | 10.0% |
| !! | ACAATGAAATAAAGAATTTA+TGG | - | chr1.4:67785732-67785751 | MS.gene21122:CDS | 15.0% |
| !! | TATCAAAAGATTTAACAGTT+TGG | + | chr1.4:67786150-67786169 | None:intergenic | 20.0% |
| !!! | AACTTCTAATATATTTTCCA+AGG | - | chr1.4:67786537-67786556 | MS.gene21122:CDS | 20.0% |
| !!! | ATAACAACAATTTTTCTTCT+TGG | - | chr1.4:67786320-67786339 | MS.gene21122:intron | 20.0% |
| !!! | ATTGTTGTTATGTAAGTTTA+AGG | + | chr1.4:67786311-67786330 | None:intergenic | 20.0% |
| !!! | TAACAACAATTTTTCTTCTT+GGG | - | chr1.4:67786321-67786340 | MS.gene21122:intron | 20.0% |
| ! | AAAAGAATTAGGAAGAAACA+TGG | + | chr1.4:67786218-67786237 | None:intergenic | 25.0% |
| ! | CCATAGGAGAAAATAAAAAA+AGG | + | chr1.4:67785565-67785584 | None:intergenic | 25.0% |
| ! | TCATTGGATAATAATATCTC+TGG | + | chr1.4:67785875-67785894 | None:intergenic | 25.0% |
| ! | TTAAGACCTGATTTAAAAAG+AGG | - | chr1.4:67785455-67785474 | MS.gene21122:CDS | 25.0% |
| ! | TTTCAAAAGGTTCAATTCAT+TGG | + | chr1.4:67785891-67785910 | None:intergenic | 25.0% |
| ! | TTTGTATAACTCATTTGAAG+TGG | + | chr1.4:67786070-67786089 | None:intergenic | 25.0% |
| !! | AACAACAATTTTTCTTCTTG+GGG | - | chr1.4:67786322-67786341 | MS.gene21122:intron | 25.0% |
| !! | AATGTTCCTCTTTTTAAATC+AGG | + | chr1.4:67785464-67785483 | None:intergenic | 25.0% |
| !! | ACAAAGAGTTTTTCATAGAT+AGG | - | chr1.4:67786011-67786030 | MS.gene21122:CDS | 25.0% |
| !! | TTCATTTTCTTCTTATCAGA+TGG | - | chr1.4:67785675-67785694 | MS.gene21122:CDS | 25.0% |
| !!! | CCTTTTTTTATTTTCTCCTA+TGG | - | chr1.4:67785562-67785581 | MS.gene21122:CDS | 25.0% |
| AAAATAGCTCCAAAACTATC+TGG | + | chr1.4:67786481-67786500 | None:intergenic | 30.0% | |
| AAAATTTCCCATCAAATCTG+AGG | + | chr1.4:67786047-67786066 | None:intergenic | 30.0% | |
| AAATTTCCCATCAAATCTGA+GGG | + | chr1.4:67786046-67786065 | None:intergenic | 30.0% | |
| AAGAAGATGAAAAACTACTG+AGG | - | chr1.4:67785220-67785239 | MS.gene21122:CDS | 30.0% | |
| CATATAACAAAGTATGGTCA+TGG | - | chr1.4:67785243-67785262 | MS.gene21122:CDS | 30.0% | |
| CTACTAGTGTTTGAATCATT+TGG | + | chr1.4:67786112-67786131 | None:intergenic | 30.0% | |
| TATGCTAATGGAGATAGAAT+TGG | + | chr1.4:67785320-67785339 | None:intergenic | 30.0% | |
| TTATTCTCAATCTCAGACAA+TGG | + | chr1.4:67785815-67785834 | None:intergenic | 30.0% | |
| TTGAACTCTCTGATTTCAAA+AGG | + | chr1.4:67785904-67785923 | None:intergenic | 30.0% | |
| TTGTTGCAGCAAAAAGAATT+AGG | + | chr1.4:67786229-67786248 | None:intergenic | 30.0% | |
| ! | AAAAGCATATGCAATGGAAA+TGG | - | chr1.4:67785976-67785995 | MS.gene21122:CDS | 30.0% |
| ! | AACTTTGTACCAGATAGTTT+TGG | - | chr1.4:67786469-67786488 | MS.gene21122:intron | 30.0% |
| ! | AGTAGTTTTTCATCTTCTTC+TGG | + | chr1.4:67785219-67785238 | None:intergenic | 30.0% |
| ! | ATTTCCATTGCATATGCTTT+TGG | + | chr1.4:67785977-67785996 | None:intergenic | 30.0% |
| ! | TTGTCTGAGATTGAGAATAA+TGG | - | chr1.4:67785815-67785834 | MS.gene21122:CDS | 30.0% |
| AAGAAGAGACTGAGACAAAA+AGG | - | chr1.4:67785767-67785786 | MS.gene21122:CDS | 35.0% | |
| ATCATACAGAAGAAGCTAAG+TGG | - | chr1.4:67786359-67786378 | MS.gene21122:intron | 35.0% | |
| ATGGTTAAGCAAGAGTGATA+AGG | - | chr1.4:67785581-67785600 | MS.gene21122:CDS | 35.0% | |
| ATGGTTGTTGATGTTGTTGT+GGG | + | chr1.4:67786199-67786218 | None:intergenic | 35.0% | |
| ATTCAACCCTCAGATTTGAT+GGG | - | chr1.4:67786037-67786056 | MS.gene21122:CDS | 35.0% | |
| CAAACACTAGTAGTAACCAT+TGG | - | chr1.4:67786119-67786138 | MS.gene21122:intron | 35.0% | |
| CAGAAGCAAGCATTAAGATA+GGG | + | chr1.4:67786414-67786433 | None:intergenic | 35.0% | |
| CTGAGGCATATAACAAAGTA+TGG | - | chr1.4:67785237-67785256 | MS.gene21122:CDS | 35.0% | |
| GATAATTTACCTGCTTGCTT+AGG | + | chr1.4:67785285-67785304 | None:intergenic | 35.0% | |
| TAACAGTTTGGTTGAACCAA+TGG | + | chr1.4:67786138-67786157 | None:intergenic | 35.0% | |
| TATCACTCTTGCTTAACCAT+AGG | + | chr1.4:67785581-67785600 | None:intergenic | 35.0% | |
| TATCAGATGGTCTCAAATTG+CGG | - | chr1.4:67785688-67785707 | MS.gene21122:CDS | 35.0% | |
| TCAGAAGCAAGCATTAAGAT+AGG | + | chr1.4:67786415-67786434 | None:intergenic | 35.0% | |
| TCATACAGAAGAAGCTAAGT+GGG | - | chr1.4:67786360-67786379 | MS.gene21122:intron | 35.0% | |
| TCTGGTACAAAGTTCATCAA+TGG | + | chr1.4:67786463-67786482 | None:intergenic | 35.0% | |
| TCTTGTTCTTGCTTTGTATG+AGG | + | chr1.4:67786505-67786524 | None:intergenic | 35.0% | |
| TTATCAGGTTTGCAAAGATG+TGG | - | chr1.4:67785401-67785420 | MS.gene21122:CDS | 35.0% | |
| TTGAACTTCATGCAGTACTA+GGG | - | chr1.4:67785510-67785529 | MS.gene21122:CDS | 35.0% | |
| ! | AACAATTTTTCTTCTTGGGG+AGG | - | chr1.4:67786325-67786344 | MS.gene21122:intron | 35.0% |
| ! | GAGAATAATGGAGAGGAAAA+TGG | - | chr1.4:67785827-67785846 | MS.gene21122:CDS | 35.0% |
| ! | TGAGATTGAGAATAATGGAG+AGG | - | chr1.4:67785820-67785839 | MS.gene21122:CDS | 35.0% |
| ! | TTCACCAAAAGCATATGCAA+TGG | - | chr1.4:67785970-67785989 | MS.gene21122:CDS | 35.0% |
| AAGAGGAACATTCTCACAAG+AGG | - | chr1.4:67785472-67785491 | MS.gene21122:CDS | 40.0% | |
| AGAAGCTTAGGAAAGGTCTA+TGG | - | chr1.4:67785190-67785209 | MS.gene21122:CDS | 40.0% | |
| CAAAGTATGGTCATGGATGT+TGG | - | chr1.4:67785250-67785269 | MS.gene21122:CDS | 40.0% | |
| CATCTCTGTGGAAACATTGT+CGG | + | chr1.4:67786269-67786288 | None:intergenic | 40.0% | |
| CATGGTTGTTGATGTTGTTG+TGG | + | chr1.4:67786200-67786219 | None:intergenic | 40.0% | |
| CATTCAACCCTCAGATTTGA+TGG | - | chr1.4:67786036-67786055 | MS.gene21122:CDS | 40.0% | |
| GACAATGGTTTATGAGTCAC+AGG | + | chr1.4:67785800-67785819 | None:intergenic | 40.0% | |
| GCATATGCAATGGAAATGGA+AGG | - | chr1.4:67785980-67785999 | MS.gene21122:CDS | 40.0% | |
| GGTACAAAGTTCATCAATGG+AGG | + | chr1.4:67786460-67786479 | None:intergenic | 40.0% | |
| GTTAAGCAAGAGTGATAAGG+AGG | - | chr1.4:67785584-67785603 | MS.gene21122:CDS | 40.0% | |
| GTTGAACTTCATGCAGTACT+AGG | - | chr1.4:67785509-67785528 | MS.gene21122:CDS | 40.0% | |
| GTTGTTACAAGCAGAAGCTT+AGG | - | chr1.4:67785178-67785197 | MS.gene21122:CDS | 40.0% | |
| TACAAGCAGAAGCTTAGGAA+AGG | - | chr1.4:67785183-67785202 | MS.gene21122:CDS | 40.0% | |
| TCTGAGTTTCTGAATGTCCT+TGG | + | chr1.4:67786557-67786576 | None:intergenic | 40.0% | |
| TTCATGCAGTACTAGGGAAT+AGG | - | chr1.4:67785516-67785535 | MS.gene21122:CDS | 40.0% | |
| TTCTTCTTGGGGAGGATTAA+TGG | - | chr1.4:67786333-67786352 | MS.gene21122:intron | 40.0% | |
| ! | CAGAAACTCAGAGAAGCTTT+TGG | - | chr1.4:67786565-67786584 | MS.gene21122:CDS | 40.0% |
| AAAGATGTGGTAAGAGCTGC+AGG | - | chr1.4:67785414-67785433 | MS.gene21122:CDS | 45.0% | |
| AAGAGTGATAAGGAGGTCGA+GGG | - | chr1.4:67785591-67785610 | MS.gene21122:CDS | 45.0% | |
| AGTTCAGTACCTAAGCAAGC+AGG | - | chr1.4:67785273-67785292 | MS.gene21122:CDS | 45.0% | |
| ATTTCATTGTCTGTCCTCCC+CGG | + | chr1.4:67785722-67785741 | None:intergenic | 45.0% | |
| TCTGTGGAAACATTGTCGGA+GGG | + | chr1.4:67786265-67786284 | None:intergenic | 45.0% | |
| CAAGAGTGATAAGGAGGTCG+AGG | - | chr1.4:67785590-67785609 | MS.gene21122:CDS | 50.0% | |
| CTCTGTGGAAACATTGTCGG+AGG | + | chr1.4:67786266-67786285 | None:intergenic | 50.0% | |
| TCAAATTGCGGCGCAATTGC+CGG | - | chr1.4:67785700-67785719 | MS.gene21122:CDS | 50.0% | |
| ! | GCTGCTAGTTCTCATCTCTG+TGG | + | chr1.4:67786281-67786300 | None:intergenic | 50.0% |
| AAATTGCGGCGCAATTGCCG+GGG | - | chr1.4:67785702-67785721 | MS.gene21122:CDS | 55.0% | |
| CAAATTGCGGCGCAATTGCC+GGG | - | chr1.4:67785701-67785720 | MS.gene21122:CDS | 55.0% | |
| GTAAGAGCTGCAGGCTTAGG+TGG | - | chr1.4:67785423-67785442 | MS.gene21122:CDS | 55.0% | |
| GTGGTAAGAGCTGCAGGCTT+AGG | - | chr1.4:67785420-67785439 | MS.gene21122:CDS | 55.0% | |
| CGAGGGCTCGATCTCACTCT+CGG | - | chr1.4:67785608-67785627 | MS.gene21122:CDS | 60.0% | |
| TTGCGGCGCAATTGCCGGGG+AGG | - | chr1.4:67785705-67785724 | MS.gene21122:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 67785162 | 67786597 | 67785162 | ID=MS.gene21122 |
| chr1.4 | mRNA | 67785162 | 67786597 | 67785162 | ID=MS.gene21122.t1;Parent=MS.gene21122 |
| chr1.4 | exon | 67786506 | 67786597 | 67786506 | ID=MS.gene21122.t1.exon1;Parent=MS.gene21122.t1 |
| chr1.4 | CDS | 67786506 | 67786597 | 67786506 | ID=cds.MS.gene21122.t1;Parent=MS.gene21122.t1 |
| chr1.4 | exon | 67785162 | 67786065 | 67785162 | ID=MS.gene21122.t1.exon2;Parent=MS.gene21122.t1 |
| chr1.4 | CDS | 67785162 | 67786065 | 67785162 | ID=cds.MS.gene21122.t1;Parent=MS.gene21122.t1 |
| Gene Sequence |
| Protein sequence |
