AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene21162

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene21162.t1 MTR_5g012390 99.714 350 1 0 1 350 1 350 0.0 715
MS.gene21162.t1 MTR_4g101130 86.000 350 49 0 1 350 1 350 0.0 618
MS.gene21162.t1 MTR_4g092690 24.514 257 161 10 39 281 46 283 4.79e-12 66.6
MS.gene21162.t1 MTR_5g014710 24.710 259 160 11 39 282 48 286 7.51e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene21162.t1 AT4G17260 77.054 353 78 1 1 350 1 353 0.0 548
MS.gene21162.t1 AT2G22780 24.809 262 162 11 39 285 44 285 1.73e-11 65.1

Find 100 sgRNAs with CRISPR-Local

Find 119 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATTGGTTCAGGAACTAATT+TGG 0.183798 5.1:-5626376 MS.gene21162:CDS
GCTGGGGCTCGCCAGATTAA+TGG 0.260199 5.1:-5626564 MS.gene21162:CDS
GACCTCTGCATCGTCACTGC+TGG 0.269339 5.1:-5626582 MS.gene21162:CDS
TATGAAGTGATCAGTTTAAA+AGG 0.279837 5.1:-5625882 MS.gene21162:CDS
GTTTAAAAGGCTACACTTCT+TGG 0.287297 5.1:-5625869 MS.gene21162:CDS
AGGGTACTCCGTCGCAAATT+TGG 0.306009 5.1:-5625841 MS.gene21162:CDS
TTCCCCTCCAACCGCGTCAT+TGG 0.328564 5.1:-5626393 MS.gene21162:CDS
ACCTCTGCATCGTCACTGCT+GGG 0.330491 5.1:-5626581 MS.gene21162:CDS
CCCTTTGCAAGTACTGAAAC+AGG 0.335982 5.1:+5625783 None:intergenic
ATACACAAAGAGGTTATAAA+TGG 0.340001 5.1:-5625909 MS.gene21162:CDS
CATCAAGATGATCAGCAATA+AGG 0.342388 5.1:+5626334 None:intergenic
TCCAACCGCGTCATTGGTTC+AGG 0.350863 5.1:-5626387 MS.gene21162:CDS
ATCATTGGGACCAGATGGTT+TGG 0.365992 5.1:-5626877 MS.gene21162:CDS
AAAATGCACAAAAGCATATC+AGG 0.366781 5.1:-5626903 MS.gene21162:CDS
CTTGGTAGAGGAGGTGTATT+GGG 0.368641 5.1:-5625711 MS.gene21162:CDS
AAGAGGAAATGAAGAGGCTT+AGG 0.376841 5.1:-5625665 MS.gene21162:CDS
CCTTTGTAGCAAATTCAATC+TGG 0.377803 5.1:+5626536 None:intergenic
ATCTGGACTCGCCATTAATC+TGG 0.381187 5.1:+5626553 None:intergenic
CACAACAAGATCTCAGTTAT+CGG 0.387966 5.1:-5626792 MS.gene21162:CDS
ATCTCGCCTCGTAGCTTGTC+AGG 0.414108 5.1:+5626687 None:intergenic
CTGAATAGTCCGTGGAGGCC+TGG 0.419596 5.1:+5626619 None:intergenic
TTTAAAAGGCTACACTTCTT+GGG 0.420092 5.1:-5625868 MS.gene21162:CDS
TACATTGCTTGGAAACTCTC+TGG 0.433042 5.1:-5626417 MS.gene21162:CDS
AAGCATATCAGGTTCATCAT+TGG 0.435410 5.1:-5626892 MS.gene21162:CDS
CATATTGTAGGCATACATAG+TGG 0.443302 5.1:-5626048 MS.gene21162:intron
ATGTGCTCACTTACATTGCT+TGG 0.446335 5.1:-5626428 MS.gene21162:CDS
AGCATATCAGGTTCATCATT+GGG 0.449605 5.1:-5626891 MS.gene21162:CDS
ATTTGGCTCGAACAATTATG+AGG 0.456393 5.1:-5625824 MS.gene21162:CDS
GCTTGGTAGAGGAGGTGTAT+TGG 0.456494 5.1:-5625712 MS.gene21162:CDS
ATGTAAGTGAGCACATCAAC+AGG 0.458914 5.1:+5626435 None:intergenic
CAAGAACAAGCTCGTCGACT+AGG 0.461803 5.1:+5626721 None:intergenic
TCTTGAGTTGCAGAATCAGT+TGG 0.461924 5.1:-5625625 MS.gene21162:CDS
CGGCGCAGGCAACGTGGGAA+TGG 0.466696 5.1:-5626772 MS.gene21162:CDS
GACTAGGTCTTGTGTTAAGA+TGG 0.470120 5.1:+5626737 None:intergenic
CAAGCTACGAGGCGAGATGC+TGG 0.472234 5.1:-5626682 MS.gene21162:CDS
CTTGAGTTGCAGAATCAGTT+GGG 0.475495 5.1:-5625624 MS.gene21162:CDS
TGAGTTAAGTCCAAACCATC+TGG 0.477368 5.1:+5626867 None:intergenic
TTGTGGCGTTTCGTTGGAGA+AGG 0.483904 5.1:+5626810 None:intergenic
GGAGGCCTGGATCTTAGTGC+GGG 0.491437 5.1:+5626632 None:intergenic
CCCTGTTTCAGTACTTGCAA+AGG 0.493370 5.1:-5625784 MS.gene21162:CDS
GTGACAGTTCAGTGGCACTA+TGG 0.496963 5.1:-5626016 MS.gene21162:CDS
TTGCCTGCACAGCTTGGTAG+AGG 0.498243 5.1:-5625723 MS.gene21162:CDS
CCTCCTCTACCAAGCTGTGC+AGG 0.499160 5.1:+5625720 None:intergenic
GAGAAGGAGGTGAGGCATGT+TGG 0.500290 5.1:+5626826 None:intergenic
GCCTCGTAGCTTGTCAGGAA+TGG 0.502021 5.1:+5626692 None:intergenic
ATCTTGTTGTGGCGTTTCGT+TGG 0.512022 5.1:+5626804 None:intergenic
CCTGTTTCAGTACTTGCAAA+GGG 0.514853 5.1:-5625783 MS.gene21162:CDS
AGAGGAAATGAAGAGGCTTA+GGG 0.518669 5.1:-5625664 MS.gene21162:CDS
CCAGATTGAATTTGCTACAA+AGG 0.522205 5.1:-5626536 MS.gene21162:CDS
GGCTACACTTCTTGGGCCAT+AGG 0.526002 5.1:-5625861 MS.gene21162:CDS
AGTGAGCACATCAACAGGAT+TGG 0.527203 5.1:+5626440 None:intergenic
ATATTGTAGGCATACATAGT+GGG 0.528803 5.1:-5626047 MS.gene21162:intron
AACCAATGACGCGGTTGGAG+GGG 0.528879 5.1:+5626391 None:intergenic
GATGATCAGCAATAAGGAAA+CGG 0.530476 5.1:+5626340 None:intergenic
CCTGCACAGCTTGGTAGAGG+AGG 0.533540 5.1:-5625720 MS.gene21162:CDS
AGGAGGTGAGGCATGTTGGA+TGG 0.541391 5.1:+5626830 None:intergenic
TGGTCAAGCATTAGTGTTGG+AGG 0.542005 5.1:-5625996 MS.gene21162:CDS
ATTAGTTTGCCTGCACAGCT+TGG 0.543386 5.1:-5625729 MS.gene21162:CDS
GAACCAATGACGCGGTTGGA+GGG 0.548021 5.1:+5626390 None:intergenic
GCTACACTTCTTGGGCCATA+GGG 0.551111 5.1:-5625860 MS.gene21162:CDS
GGTTCATCATTGGGACCAGA+TGG 0.553995 5.1:-5626882 MS.gene21162:CDS
TGGCGTTTCGTTGGAGAAGG+AGG 0.556154 5.1:+5626813 None:intergenic
TGAACCAATGACGCGGTTGG+AGG 0.560747 5.1:+5626389 None:intergenic
ATTTGCGACGGAGTACCCTA+TGG 0.561442 5.1:+5625845 None:intergenic
TCCTGAACCAATGACGCGGT+TGG 0.564746 5.1:+5626386 None:intergenic
ATGTTGGATGGACTTGAAGA+AGG 0.565700 5.1:+5626842 None:intergenic
CACTAAGATCCAGGCCTCCA+CGG 0.566976 5.1:-5626628 MS.gene21162:CDS
AAGATCTCAGTTATCGGCGC+AGG 0.570093 5.1:-5626786 MS.gene21162:CDS
GAACAATTATGAGGAATCAA+AGG 0.573740 5.1:-5625815 MS.gene21162:CDS
ACGGACTATTCAGTGACGAT+GGG 0.573834 5.1:-5626609 MS.gene21162:CDS
GCTAGAGAACATACACAAAG+AGG 0.577375 5.1:-5625919 MS.gene21162:CDS
TAATATTCATATGACTGAAG+AGG 0.582508 5.1:-5625682 MS.gene21162:CDS
CTATGGTCAAGCATTAGTGT+TGG 0.582886 5.1:-5625999 MS.gene21162:CDS
TGTTAATGCTCAAGATGTGC+AGG 0.582927 5.1:-5626313 MS.gene21162:intron
CACGGACTATTCAGTGACGA+TGG 0.583882 5.1:-5626610 MS.gene21162:CDS
CCCCAGCAGTGACGATGCAG+AGG 0.586925 5.1:+5626580 None:intergenic
GTTATCGGCGCAGGCAACGT+GGG 0.604202 5.1:-5626777 MS.gene21162:CDS
TGGAGGCCTGGATCTTAGTG+CGG 0.606000 5.1:+5626631 None:intergenic
CCTCTGCATCGTCACTGCTG+GGG 0.608669 5.1:-5626580 MS.gene21162:CDS
TATTGTAGGCATACATAGTG+GGG 0.617018 5.1:-5626046 MS.gene21162:intron
GAGGCCTGGATCTTAGTGCG+GGG 0.617664 5.1:+5626633 None:intergenic
AGTTATCGGCGCAGGCAACG+TGG 0.623394 5.1:-5626778 MS.gene21162:CDS
AGGCCTGGATCTTAGTGCGG+GGG 0.630067 5.1:+5626634 None:intergenic
TGACTGAAGAGGAAATGAAG+AGG 0.635441 5.1:-5625671 MS.gene21162:CDS
GGAACATGGTGACAGTTCAG+TGG 0.640556 5.1:-5626024 MS.gene21162:CDS
ACAATTAGAAGAACACAATG+AGG 0.641975 5.1:+5626465 None:intergenic
TGTTCGAGCCAAATTTGCGA+CGG 0.645980 5.1:+5625833 None:intergenic
TGAGGAGAGTAACGAACCAA+AGG 0.646562 5.1:+5626483 None:intergenic
CGATAACTGAGATCTTGTTG+TGG 0.649606 5.1:+5626793 None:intergenic
GGAGAGTAACGAACCAAAGG+TGG 0.659475 5.1:+5626486 None:intergenic
AATAAGGAAACGGAAGCGAG+AGG 0.662348 5.1:+5626350 None:intergenic
CCTTTGCAAGTACTGAAACA+GGG 0.664039 5.1:+5625784 None:intergenic
CATCGTCACTGAATAGTCCG+TGG 0.664896 5.1:+5626611 None:intergenic
TTTCGTTGGAGAAGGAGGTG+AGG 0.681321 5.1:+5626818 None:intergenic
ATTGTAGGCATACATAGTGG+GGG 0.689959 5.1:-5626045 MS.gene21162:intron
CGGACTATTCAGTGACGATG+GGG 0.708696 5.1:-5626608 MS.gene21162:CDS
GATGCTGGATCTACAGCACG+CGG 0.713694 5.1:-5626667 MS.gene21162:CDS
ACCATTCCTGACAAGCTACG+AGG 0.715464 5.1:-5626693 MS.gene21162:CDS
CGTCACTGAATAGTCCGTGG+AGG 0.718203 5.1:+5626614 None:intergenic
TAGTTCCTGAACCAATGACG+CGG 0.731349 5.1:+5626382 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATATATTTTTTAAGCAATA+AGG + chr5.1:5626242-5626261 None:intergenic 10.0%
!! TAAAAAAATTATACCACCTT+TGG - chr5.1:5626018-5626037 MS.gene21162:CDS 20.0%
!!! TCAGTTTAATTTCATATTGT+AGG - chr5.1:5626457-5626476 MS.gene21162:CDS 20.0%
! ATACACAAAGAGGTTATAAA+TGG - chr5.1:5626608-5626627 MS.gene21162:CDS 25.0%
! TAATATTCATATGACTGAAG+AGG - chr5.1:5626835-5626854 MS.gene21162:CDS 25.0%
! TATGAAGTGATCAGTTTAAA+AGG - chr5.1:5626635-5626654 MS.gene21162:CDS 25.0%
ACAATTAGAAGAACACAATG+AGG + chr5.1:5626055-5626074 None:intergenic 30.0%
ATATTGTAGGCATACATAGT+GGG - chr5.1:5626470-5626489 MS.gene21162:CDS 30.0%
GAACAATTATGAGGAATCAA+AGG - chr5.1:5626702-5626721 MS.gene21162:CDS 30.0%
TTTAAAAGGCTACACTTCTT+GGG - chr5.1:5626649-5626668 MS.gene21162:CDS 30.0%
! TTGCTATTACATTGCATGTT+TGG + chr5.1:5626371-5626390 None:intergenic 30.0%
!! TCGTGAGTTTGCTAAAATTA+CGG + chr5.1:5626343-5626362 None:intergenic 30.0%
AAGCATATCAGGTTCATCAT+TGG - chr5.1:5625625-5625644 MS.gene21162:CDS 35.0%
AGCATATCAGGTTCATCATT+GGG - chr5.1:5625626-5625645 MS.gene21162:CDS 35.0%
CACAACAAGATCTCAGTTAT+CGG - chr5.1:5625725-5625744 MS.gene21162:CDS 35.0%
CATATTGTAGGCATACATAG+TGG - chr5.1:5626469-5626488 MS.gene21162:CDS 35.0%
CATCAAGATGATCAGCAATA+AGG + chr5.1:5626186-5626205 None:intergenic 35.0%
CCTTTGTAGCAAATTCAATC+TGG + chr5.1:5625984-5626003 None:intergenic 35.0%
GATGATCAGCAATAAGGAAA+CGG + chr5.1:5626180-5626199 None:intergenic 35.0%
GTTTAAAAGGCTACACTTCT+TGG - chr5.1:5626648-5626667 MS.gene21162:CDS 35.0%
TATTGTAGGCATACATAGTG+GGG - chr5.1:5626471-5626490 MS.gene21162:CDS 35.0%
! ATTTGGCTCGAACAATTATG+AGG - chr5.1:5626693-5626712 MS.gene21162:CDS 35.0%
! GTACTTGCAAAGGGATTTTA+TGG - chr5.1:5626743-5626762 MS.gene21162:CDS 35.0%
! TAAAATTACGGCTGCGATTT+TGG + chr5.1:5626331-5626350 None:intergenic 35.0%
!! AAGGGATTTTATGGCATTGA+TGG - chr5.1:5626752-5626771 MS.gene21162:CDS 35.0%
!! CATTGGTTCAGGAACTAATT+TGG - chr5.1:5626141-5626160 MS.gene21162:intron 35.0%
!! CCAGATTGAATTTGCTACAA+AGG - chr5.1:5625981-5626000 MS.gene21162:CDS 35.0%
AAGAGGAAATGAAGAGGCTT+AGG - chr5.1:5626852-5626871 MS.gene21162:CDS 40.0%
AGAGGAAATGAAGAGGCTTA+GGG - chr5.1:5626853-5626872 MS.gene21162:CDS 40.0%
ATGTAAGTGAGCACATCAAC+AGG + chr5.1:5626085-5626104 None:intergenic 40.0%
ATGTGCTCACTTACATTGCT+TGG - chr5.1:5626089-5626108 MS.gene21162:intron 40.0%
ATTGTAGGCATACATAGTGG+GGG - chr5.1:5626472-5626491 MS.gene21162:CDS 40.0%
CCTGTTTCAGTACTTGCAAA+GGG - chr5.1:5626734-5626753 MS.gene21162:CDS 40.0%
CGATAACTGAGATCTTGTTG+TGG + chr5.1:5625727-5625746 None:intergenic 40.0%
CTATGGTCAAGCATTAGTGT+TGG - chr5.1:5626518-5626537 MS.gene21162:CDS 40.0%
GCTAGAGAACATACACAAAG+AGG - chr5.1:5626598-5626617 MS.gene21162:CDS 40.0%
TACATTGCTTGGAAACTCTC+TGG - chr5.1:5626100-5626119 MS.gene21162:intron 40.0%
TGACTGAAGAGGAAATGAAG+AGG - chr5.1:5626846-5626865 MS.gene21162:CDS 40.0%
TGAGTTAAGTCCAAACCATC+TGG + chr5.1:5625653-5625672 None:intergenic 40.0%
TGTTAATGCTCAAGATGTGC+AGG - chr5.1:5626204-5626223 MS.gene21162:intron 40.0%
TTCTCCAAAAAGCTTAGCAC+TGG + chr5.1:5626551-5626570 None:intergenic 40.0%
! ATGTTGGATGGACTTGAAGA+AGG + chr5.1:5625678-5625697 None:intergenic 40.0%
! CTTGAGTTGCAGAATCAGTT+GGG - chr5.1:5626893-5626912 MS.gene21162:CDS 40.0%
! GACTAGGTCTTGTGTTAAGA+TGG + chr5.1:5625783-5625802 None:intergenic 40.0%
! TCTTGAGTTGCAGAATCAGT+TGG - chr5.1:5626892-5626911 MS.gene21162:CDS 40.0%
! TGTTCCAGTGCTAAGCTTTT+TGG - chr5.1:5626544-5626563 MS.gene21162:CDS 40.0%
!! CCTTTGCAAGTACTGAAACA+GGG + chr5.1:5626736-5626755 None:intergenic 40.0%
!!! GGATTTTATGGCATTGATGG+TGG - chr5.1:5626755-5626774 MS.gene21162:CDS 40.0%
AATAAGGAAACGGAAGCGAG+AGG + chr5.1:5626170-5626189 None:intergenic 45.0%
AGTGAGCACATCAACAGGAT+TGG + chr5.1:5626080-5626099 None:intergenic 45.0%
ATCATTGGGACCAGATGGTT+TGG - chr5.1:5625640-5625659 MS.gene21162:CDS 45.0%
ATCTGGACTCGCCATTAATC+TGG + chr5.1:5625967-5625986 None:intergenic 45.0%
CCCTGTTTCAGTACTTGCAA+AGG - chr5.1:5626733-5626752 MS.gene21162:CDS 45.0%
TAGTTCCTGAACCAATGACG+CGG + chr5.1:5626138-5626157 None:intergenic 45.0%
TGAGGAGAGTAACGAACCAA+AGG + chr5.1:5626037-5626056 None:intergenic 45.0%
TGTTCGAGCCAAATTTGCGA+CGG + chr5.1:5626687-5626706 None:intergenic 45.0%
! ACGGACTATTCAGTGACGAT+GGG - chr5.1:5625908-5625927 MS.gene21162:CDS 45.0%
! ATCTTGTTGTGGCGTTTCGT+TGG + chr5.1:5625716-5625735 None:intergenic 45.0%
! CTTGGTAGAGGAGGTGTATT+GGG - chr5.1:5626806-5626825 MS.gene21162:CDS 45.0%
! TGGTCAAGCATTAGTGTTGG+AGG - chr5.1:5626521-5626540 MS.gene21162:CDS 45.0%
! TTGCGCGCATGTTTGGTAAT+TGG - chr5.1:5626285-5626304 MS.gene21162:intron 45.0%
!! ATTAGTTTGCCTGCACAGCT+TGG - chr5.1:5626788-5626807 MS.gene21162:CDS 45.0%
!! CCCTTTGCAAGTACTGAAAC+AGG + chr5.1:5626737-5626756 None:intergenic 45.0%
AAACATGCGCGCAAGTGCAA+TGG + chr5.1:5626279-5626298 None:intergenic 50.0%
AACATGCGCGCAAGTGCAAT+GGG + chr5.1:5626278-5626297 None:intergenic 50.0%
AAGATCTCAGTTATCGGCGC+AGG - chr5.1:5625731-5625750 MS.gene21162:CDS 50.0%
ACCATTCCTGACAAGCTACG+AGG - chr5.1:5625824-5625843 MS.gene21162:CDS 50.0%
ATTGCACTTGCGCGCATGTT+TGG - chr5.1:5626278-5626297 MS.gene21162:intron 50.0%
CATCGTCACTGAATAGTCCG+TGG + chr5.1:5625909-5625928 None:intergenic 50.0%
CGGACTATTCAGTGACGATG+GGG - chr5.1:5625909-5625928 MS.gene21162:CDS 50.0%
GCTACACTTCTTGGGCCATA+GGG - chr5.1:5626657-5626676 MS.gene21162:CDS 50.0%
GGAACATGGTGACAGTTCAG+TGG - chr5.1:5626493-5626512 MS.gene21162:CDS 50.0%
GGAGAGTAACGAACCAAAGG+TGG + chr5.1:5626034-5626053 None:intergenic 50.0%
GGTTCATCATTGGGACCAGA+TGG - chr5.1:5625635-5625654 MS.gene21162:CDS 50.0%
GTGACAGTTCAGTGGCACTA+TGG - chr5.1:5626501-5626520 MS.gene21162:CDS 50.0%
TTGTGGCGTTTCGTTGGAGA+AGG + chr5.1:5625710-5625729 None:intergenic 50.0%
TTTCGTTGGAGAAGGAGGTG+AGG + chr5.1:5625702-5625721 None:intergenic 50.0%
! AGGGTACTCCGTCGCAAATT+TGG - chr5.1:5626676-5626695 MS.gene21162:CDS 50.0%
! ATTTGCGACGGAGTACCCTA+TGG + chr5.1:5626675-5626694 None:intergenic 50.0%
! CAAGAACAAGCTCGTCGACT+AGG + chr5.1:5625799-5625818 None:intergenic 50.0%
! CACGGACTATTCAGTGACGA+TGG - chr5.1:5625907-5625926 MS.gene21162:CDS 50.0%
! GCATACATAGTGGGGGAACA+TGG - chr5.1:5626479-5626498 MS.gene21162:CDS 50.0%
! GCTTGGTAGAGGAGGTGTAT+TGG - chr5.1:5626805-5626824 MS.gene21162:CDS 50.0%
AACCAATGACGCGGTTGGAG+GGG + chr5.1:5626129-5626148 None:intergenic 55.0%
ACATGCGCGCAAGTGCAATG+GGG + chr5.1:5626277-5626296 None:intergenic 55.0%
ACCTCTGCATCGTCACTGCT+GGG - chr5.1:5625936-5625955 MS.gene21162:CDS 55.0%
AGGAGGTGAGGCATGTTGGA+TGG + chr5.1:5625690-5625709 None:intergenic 55.0%
CACTAAGATCCAGGCCTCCA+CGG - chr5.1:5625889-5625908 MS.gene21162:CDS 55.0%
CGTCACTGAATAGTCCGTGG+AGG + chr5.1:5625906-5625925 None:intergenic 55.0%
GAACCAATGACGCGGTTGGA+GGG + chr5.1:5626130-5626149 None:intergenic 55.0%
GAGAAGGAGGTGAGGCATGT+TGG + chr5.1:5625694-5625713 None:intergenic 55.0%
GATGCTGGATCTACAGCACG+CGG - chr5.1:5625850-5625869 MS.gene21162:CDS 55.0%
GGCTACACTTCTTGGGCCAT+AGG - chr5.1:5626656-5626675 MS.gene21162:CDS 55.0%
TCCTGAACCAATGACGCGGT+TGG + chr5.1:5626134-5626153 None:intergenic 55.0%
TGAACCAATGACGCGGTTGG+AGG + chr5.1:5626131-5626150 None:intergenic 55.0%
TGGAGGCCTGGATCTTAGTG+CGG + chr5.1:5625889-5625908 None:intergenic 55.0%
TGGCGTTTCGTTGGAGAAGG+AGG + chr5.1:5625707-5625726 None:intergenic 55.0%
TTGCCTGCACAGCTTGGTAG+AGG - chr5.1:5626794-5626813 MS.gene21162:CDS 55.0%
! ATCTCGCCTCGTAGCTTGTC+AGG + chr5.1:5625833-5625852 None:intergenic 55.0%
! GCCTCGTAGCTTGTCAGGAA+TGG + chr5.1:5625828-5625847 None:intergenic 55.0%
!! TCCAACCGCGTCATTGGTTC+AGG - chr5.1:5626130-5626149 MS.gene21162:intron 55.0%
AGGCCTGGATCTTAGTGCGG+GGG + chr5.1:5625886-5625905 None:intergenic 60.0%
AGTTATCGGCGCAGGCAACG+TGG - chr5.1:5625739-5625758 MS.gene21162:CDS 60.0%
CAAGCTACGAGGCGAGATGC+TGG - chr5.1:5625835-5625854 MS.gene21162:CDS 60.0%
CCTCCTCTACCAAGCTGTGC+AGG + chr5.1:5626800-5626819 None:intergenic 60.0%
CCTCTGCATCGTCACTGCTG+GGG - chr5.1:5625937-5625956 MS.gene21162:CDS 60.0%
CCTGCACAGCTTGGTAGAGG+AGG - chr5.1:5626797-5626816 MS.gene21162:CDS 60.0%
CTGAATAGTCCGTGGAGGCC+TGG + chr5.1:5625901-5625920 None:intergenic 60.0%
GACCTCTGCATCGTCACTGC+TGG - chr5.1:5625935-5625954 MS.gene21162:CDS 60.0%
GAGGCCTGGATCTTAGTGCG+GGG + chr5.1:5625887-5625906 None:intergenic 60.0%
GATCTTAGTGCGGGGGAGGA+AGG + chr5.1:5625879-5625898 None:intergenic 60.0%
GCTGGGGCTCGCCAGATTAA+TGG - chr5.1:5625953-5625972 MS.gene21162:CDS 60.0%
GGAGGCCTGGATCTTAGTGC+GGG + chr5.1:5625888-5625907 None:intergenic 60.0%
GTTATCGGCGCAGGCAACGT+GGG - chr5.1:5625740-5625759 MS.gene21162:CDS 60.0%
TTCCCCTCCAACCGCGTCAT+TGG - chr5.1:5626124-5626143 MS.gene21162:intron 60.0%
CCCCAGCAGTGACGATGCAG+AGG + chr5.1:5625940-5625959 None:intergenic 65.0%
CCTCCCCCGCACTAAGATCC+AGG - chr5.1:5625880-5625899 MS.gene21162:CDS 65.0%
CCTGGATCTTAGTGCGGGGG+AGG + chr5.1:5625883-5625902 None:intergenic 65.0%
CGGCGCAGGCAACGTGGGAA+TGG - chr5.1:5625745-5625764 MS.gene21162:CDS 70.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 5625617 5626922 5625617 ID=MS.gene21162
chr5.1 mRNA 5625617 5626922 5625617 ID=MS.gene21162.t1;Parent=MS.gene21162
chr5.1 exon 5626314 5626922 5626314 ID=MS.gene21162.t1.exon1;Parent=MS.gene21162.t1
chr5.1 CDS 5626314 5626922 5626314 ID=cds.MS.gene21162.t1;Parent=MS.gene21162.t1
chr5.1 exon 5625617 5626060 5625617 ID=MS.gene21162.t1.exon2;Parent=MS.gene21162.t1
chr5.1 CDS 5625617 5626060 5625617 ID=cds.MS.gene21162.t1;Parent=MS.gene21162.t1
Gene Sequence

>MS.gene21162

ATGCACAAAAGCATATCAGGTTCATCATTGGGACCAGATGGTTTGGACTTAACTCAGTCCTTCTTCAAGTCCATCCAACATGCCTCACCTCCTTCTCCAACGAAACGCCACAACAAGATCTCAGTTATCGGCGCAGGCAACGTGGGAATGGCTATAGCTCAAACCATCTTAACACAAGACCTAGTCGACGAGCTTGTTCTTGTCGACACCATTCCTGACAAGCTACGAGGCGAGATGCTGGATCTACAGCACGCGGCAGCCTTCCTCCCCCGCACTAAGATCCAGGCCTCCACGGACTATTCAGTGACGATGGGGTCTGACCTCTGCATCGTCACTGCTGGGGCTCGCCAGATTAATGGCGAGTCCAGATTGAATTTGCTACAAAGGAATGTTGCTTTGTTTAAAAAAATTATACCACCTTTGGTTCGTTACTCTCCTCATTGTGTTCTTCTAATTGTTTCCAATCCTGTTGATGTGCTCACTTACATTGCTTGGAAACTCTCTGGTTTCCCCTCCAACCGCGTCATTGGTTCAGGAACTAATTTGGACTCCTCTCGCTTCCGTTTCCTTATTGCTGATCATCTTGATGTTAATGCTCAAGATGTGCAGGTACAACTTTTTTCCTTATTGCTTAAAAAATATATATCGTCGATCATTCCCCATTGCACTTGCGCGCATGTTTGGTAATTGGTATCACAGTGAATAGTTTTTCCAAAATCGCAGCCGTAATTTTAGCAAACTCACGATAATACCAAACATGCAATGTAATAGCAATTGCAAAACGTAATTTAGAACTAACACTGATCACGAACGAACACACATAGTAAACTCAATATTTTATCAGTTTAATTTCATATTGTAGGCATACATAGTGGGGGAACATGGTGACAGTTCAGTGGCACTATGGTCAAGCATTAGTGTTGGAGGTGTTCCAGTGCTAAGCTTTTTGGAGAAACAACAAATAGCTTACGAGAAAGAAATGCTAGAGAACATACACAAAGAGGTTATAAATGGCGCATATGAAGTGATCAGTTTAAAAGGCTACACTTCTTGGGCCATAGGGTACTCCGTCGCAAATTTGGCTCGAACAATTATGAGGAATCAAAGGAAGATTCACCCTGTTTCAGTACTTGCAAAGGGATTTTATGGCATTGATGGTGGAGAAGTGTTTATTAGTTTGCCTGCACAGCTTGGTAGAGGAGGTGTATTGGGTGTGACTAATATTCATATGACTGAAGAGGAAATGAAGAGGCTTAGGGACTCTGCTATCACTATTCTTGAGTTGCAGAATCAGTTGGGTATTTGA
Protein sequence

>MS.gene21162.t1

MHKSISGSSLGPDGLDLTQSFFKSIQHASPPSPTKRHNKISVIGAGNVGMAIAQTILTQDLVDELVLVDTIPDKLRGEMLDLQHAAAFLPRTKIQASTDYSVTMGSDLCIVTAGARQINGESRLNLLQRNVALFKKIIPPLVRYSPHCVLLIVSNPVDVLTYIAWKLSGFPSNRVIGSGTNLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPVLSFLEKQQIAYEKEMLENIHKEVINGAYEVISLKGYTSWAIGYSVANLARTIMRNQRKIHPVSVLAKGFYGIDGGEVFISLPAQLGRGGVLGVTNIHMTEEEMKRLRDSAITILELQNQLGI