Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22410.t1 | XP_003625674.1 | 100 | 64 | 0 | 0 | 1 | 64 | 1 | 64 | 4.80E-29 | 136.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22410.t1 | G7L3V3 | 100.0 | 64 | 0 | 0 | 1 | 64 | 1 | 64 | 3.5e-29 | 136.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050020 | MS.gene22410 | 0.837814 | 4.16E-57 | -1.69E-46 |
| MS.gene050298 | MS.gene22410 | 0.823375 | 1.43E-53 | -1.69E-46 |
| MS.gene050563 | MS.gene22410 | 0.868993 | 4.32E-66 | -1.69E-46 |
| MS.gene050584 | MS.gene22410 | 0.845194 | 4.73E-59 | -1.69E-46 |
| MS.gene050585 | MS.gene22410 | 0.837153 | 6.15E-57 | -1.69E-46 |
| MS.gene050587 | MS.gene22410 | 0.845065 | 5.12E-59 | -1.69E-46 |
| MS.gene050807 | MS.gene22410 | 0.807506 | 4.89E-50 | -1.69E-46 |
| MS.gene05143 | MS.gene22410 | 0.810576 | 1.08E-50 | -1.69E-46 |
| MS.gene051789 | MS.gene22410 | 0.83792 | 3.91E-57 | -1.69E-46 |
| MS.gene052142 | MS.gene22410 | 0.862686 | 4.25E-64 | -1.69E-46 |
| MS.gene052698 | MS.gene22410 | 0.830048 | 3.66E-55 | -1.69E-46 |
| MS.gene053074 | MS.gene22410 | 0.836698 | 8.03E-57 | -1.69E-46 |
| MS.gene053383 | MS.gene22410 | 0.885845 | 5.72E-72 | -1.69E-46 |
| MS.gene053563 | MS.gene22410 | 0.802082 | 6.64E-49 | -1.69E-46 |
| MS.gene05456 | MS.gene22410 | 0.880947 | 3.61E-70 | -1.69E-46 |
| MS.gene055359 | MS.gene22410 | 0.870873 | 1.05E-66 | -1.69E-46 |
| MS.gene058168 | MS.gene22410 | 0.812144 | 4.91E-51 | -1.69E-46 |
| MS.gene059006 | MS.gene22410 | 0.868236 | 7.59E-66 | -1.69E-46 |
| MS.gene059632 | MS.gene22410 | 0.806132 | 9.54E-50 | -1.69E-46 |
| MS.gene059768 | MS.gene22410 | 0.846206 | 2.51E-59 | -1.69E-46 |
| MS.gene060691 | MS.gene22410 | 0.843221 | 1.60E-58 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22410.t1 | MTR_7g101850 | 100.000 | 64 | 0 | 0 | 1 | 64 | 1 | 64 | 3.78e-43 | 133 |
| MS.gene22410.t1 | MTR_1g063830 | 57.143 | 63 | 26 | 1 | 1 | 62 | 3 | 65 | 2.31e-20 | 76.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22410.t1 | AT2G36985 | 72.727 | 44 | 12 | 0 | 19 | 62 | 4 | 47 | 5.20e-20 | 75.1 |
| MS.gene22410.t1 | AT3G53232 | 70.588 | 34 | 10 | 0 | 24 | 57 | 11 | 44 | 1.16e-12 | 56.6 |
Find 10 sgRNAs with CRISPR-Local
Find 10 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GATGCTGATGAGTACTATTT+TGG | 0.208088 | 7.3:+11579431 | None:intergenic |
| TGACCAAGTGACTACACTTT+TGG | 0.325622 | 7.3:+11579371 | None:intergenic |
| TGTGATCCGTGCAAGTCTTT+TGG | 0.334940 | 7.3:-11579394 | MS.gene22410:CDS |
| AAAATCATTCATGGCAGTAA+AGG | 0.489511 | 7.3:-11579473 | None:intergenic |
| TGGCCAAAAGTGTAGTCACT+TGG | 0.560139 | 7.3:-11579374 | MS.gene22410:CDS |
| CATCAGCATCAAGAACAAGA+AGG | 0.581729 | 7.3:-11579418 | MS.gene22410:CDS |
| ACAAAAGCATGGCGATGCAA+CGG | 0.595123 | 7.3:+11579317 | None:intergenic |
| ATCAGCATCAAGAACAAGAA+GGG | 0.607865 | 7.3:-11579417 | MS.gene22410:CDS |
| TTCATGATGACACAAAAGCA+TGG | 0.650703 | 7.3:+11579306 | None:intergenic |
| TAAAGGAAATTAGCAGCACA+AGG | 0.665511 | 7.3:-11579456 | MS.gene22410:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ATCAGCATCAAGAACAAGAA+GGG | - | chr7.3:11579337-11579356 | MS.gene22410:CDS | 35.0% | |
| TAAAGGAAATTAGCAGCACA+AGG | - | chr7.3:11579298-11579317 | MS.gene22410:CDS | 35.0% | |
| TTCATGATGACACAAAAGCA+TGG | + | chr7.3:11579451-11579470 | None:intergenic | 35.0% | |
| ! | GATGCTGATGAGTACTATTT+TGG | + | chr7.3:11579326-11579345 | None:intergenic | 35.0% |
| CATCAGCATCAAGAACAAGA+AGG | - | chr7.3:11579336-11579355 | MS.gene22410:CDS | 40.0% | |
| ! | TGACCAAGTGACTACACTTT+TGG | + | chr7.3:11579386-11579405 | None:intergenic | 40.0% |
| ! | TTTTGGCCAAAAGACTTGCA+CGG | + | chr7.3:11579369-11579388 | None:intergenic | 40.0% |
| ! | ACAAAAGCATGGCGATGCAA+CGG | + | chr7.3:11579440-11579459 | None:intergenic | 45.0% |
| ! | TGGCCAAAAGTGTAGTCACT+TGG | - | chr7.3:11579380-11579399 | MS.gene22410:CDS | 45.0% |
| ! | TGTGATCCGTGCAAGTCTTT+TGG | - | chr7.3:11579360-11579379 | MS.gene22410:CDS | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 11579291 | 11579485 | 11579291 | ID=MS.gene22410 |
| chr7.3 | mRNA | 11579291 | 11579485 | 11579291 | ID=MS.gene22410.t1;Parent=MS.gene22410 |
| chr7.3 | exon | 11579291 | 11579485 | 11579291 | ID=MS.gene22410.t1.exon1;Parent=MS.gene22410.t1 |
| chr7.3 | CDS | 11579291 | 11579485 | 11579291 | ID=cds.MS.gene22410.t1;Parent=MS.gene22410.t1 |
| Gene Sequence |
| Protein sequence |
