AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene22669


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene22669.t1 MTR_7g114920 92.913 127 9 0 60 186 1 127 5.31e-87 252
MS.gene22669.t1 MTR_1g100773 75.556 135 24 2 60 186 1 134 5.49e-70 209
MS.gene22669.t1 MTR_2g054650 53.125 128 45 5 62 185 4 120 7.84e-34 117
MS.gene22669.t1 MTR_4g053440 37.500 152 66 3 64 186 13 164 6.30e-30 108
MS.gene22669.t1 MTR_2g086190 43.056 144 66 5 56 186 4 144 6.97e-30 107
MS.gene22669.t1 MTR_1g060380 40.972 144 69 3 56 183 4 147 6.52e-29 105
MS.gene22669.t1 MTR_7g104320 38.776 147 61 3 64 183 15 159 2.32e-28 104
MS.gene22669.t1 MTR_0249s0070 33.537 164 82 4 47 183 5 168 9.23e-25 95.5
MS.gene22669.t1 MTR_0249s0070 34.839 155 74 4 56 183 6 160 1.10e-24 95.1
MS.gene22669.t1 MTR_2g098160 35.570 149 69 3 62 183 15 163 1.08e-23 92.4
MS.gene22669.t1 MTR_7g092400 35.333 150 68 4 62 183 103 251 9.06e-23 92.0
MS.gene22669.t1 MTR_3g028010 30.921 152 98 2 39 183 3 154 1.54e-22 89.4
MS.gene22669.t1 MTR_4g065570 34.646 127 61 3 79 183 2 128 1.10e-18 78.6
MS.gene22669.t1 MTR_8g036980 53.846 52 24 0 132 183 91 142 1.14e-16 73.6
MS.gene22669.t1 MTR_3g025570 50.000 46 23 0 125 170 2 47 4.43e-11 57.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene22669.t1 AT1G12440 42.361 144 61 4 62 183 16 159 2.78e-32 114
MS.gene22669.t1 AT1G12440 42.361 144 61 4 62 183 16 159 2.78e-32 114
MS.gene22669.t1 AT1G12440 42.361 144 61 4 62 183 16 159 2.78e-32 114
MS.gene22669.t1 AT4G12040 40.397 151 61 4 62 183 16 166 2.57e-30 109
MS.gene22669.t1 AT4G12040 40.397 151 61 4 62 183 16 166 2.57e-30 109
MS.gene22669.t1 AT4G22820 35.000 160 77 2 51 183 8 167 5.55e-29 106
MS.gene22669.t1 AT4G22820 35.000 160 77 2 51 183 8 167 5.55e-29 106
MS.gene22669.t1 AT4G14225 44.800 125 59 4 62 186 5 119 2.36e-26 97.8
MS.gene22669.t1 AT4G25380 42.857 119 61 2 65 183 10 121 1.38e-25 96.3
MS.gene22669.t1 AT3G52800 39.241 158 66 5 56 183 4 161 2.04e-25 97.1
MS.gene22669.t1 AT3G52800 39.241 158 66 5 56 183 4 161 2.04e-25 97.1
MS.gene22669.t1 AT2G36320 38.732 142 69 4 64 187 15 156 2.07e-25 96.7
MS.gene22669.t1 AT2G27580 34.000 150 66 3 62 183 10 154 5.61e-24 92.8
MS.gene22669.t1 AT2G27580 34.000 150 66 3 62 183 10 154 5.61e-24 92.8
MS.gene22669.t1 AT3G12630 36.364 121 70 3 70 183 31 151 1.52e-20 84.0
MS.gene22669.t1 AT1G51200 34.000 150 71 3 62 183 15 164 8.00e-20 82.4
MS.gene22669.t1 AT1G51200 34.000 150 71 3 62 183 15 164 8.00e-20 82.4
MS.gene22669.t1 AT1G51200 34.000 150 71 3 62 183 15 164 8.00e-20 82.4
MS.gene22669.t1 AT1G51200 34.000 150 71 3 62 183 15 164 8.00e-20 82.4

Find 46 sgRNAs with CRISPR-Local

Find 72 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTTTGCATGTATGTTCTTC+TGG 0.205127 7.2:-2358164 None:intergenic
GCCAATGGTTGTGGTTTCTA+TGG 0.283691 7.2:+2357882 MS.gene22669:CDS
CTTTGCATGTATGTTCTTCT+GGG 0.309729 7.2:-2358163 None:intergenic
AAGCCGATGACTCAAGAATA+AGG 0.356929 7.2:-2357962 None:intergenic
GAACAAAGGTTCTTGTTTGA+AGG 0.378685 7.2:-2357909 None:intergenic
GAAACAAAATCCTTTGTGTA+TGG 0.383600 7.2:+2358220 MS.gene22669:CDS
GACGGAGAAGGAGAGGGGTT+TGG 0.390926 7.2:+2357325 MS.gene22669:CDS
AAAGTGGATTTAAAAGAGAT+TGG 0.430845 7.2:+2358182 MS.gene22669:CDS
AGTGAGGGTTATGGCTTTGA+CGG 0.436346 7.2:+2357307 None:intergenic
TCACAAATATCATTGATACT+AGG 0.441086 7.2:-2358002 None:intergenic
GTGCGTAGTTTAAGAGAGGA+AGG 0.456139 7.2:+2357347 MS.gene22669:CDS
TCACTTTGTGCCAATGGTTG+TGG 0.466305 7.2:+2357873 MS.gene22669:CDS
AAACCTTATTCTTGAGTCAT+CGG 0.472750 7.2:+2357959 MS.gene22669:CDS
TTGATACTAGGGTTCTTTGA+AGG 0.481391 7.2:-2357990 None:intergenic
AACAAAATCCTTTGTGTATG+GGG 0.487990 7.2:+2358222 MS.gene22669:CDS
AAACAAAATCCTTTGTGTAT+GGG 0.488764 7.2:+2358221 MS.gene22669:CDS
CACAAATATCATTGATACTA+GGG 0.493722 7.2:-2358001 None:intergenic
GGTTATGGCTTTGACGGAGA+AGG 0.516948 7.2:+2357313 None:intergenic
GTTCCGTCACTTTGTGCCAA+TGG 0.523599 7.2:+2357867 MS.gene22669:CDS
TAAGAGAGGAAGGGGTGCGT+AGG 0.523850 7.2:+2357357 MS.gene22669:CDS
GGCTTTGACGGAGAAGGAGA+GGG 0.532470 7.2:+2357319 MS.gene22669:CDS
TGCGTAGTTTAAGAGAGGAA+GGG 0.537577 7.2:+2357348 MS.gene22669:CDS
CATTGTAACACTTTGAACAA+AGG 0.545148 7.2:-2357923 None:intergenic
AGGAGAAGAAAACTAGGTGC+AGG 0.548806 7.2:+2358066 MS.gene22669:CDS
TGGTGGTGATGAGAGGGATA+AGG 0.549250 7.2:+2357421 MS.gene22669:CDS
ACCATAGAAACCACAACCAT+TGG 0.550076 7.2:-2357883 None:intergenic
TTTGGTGCGTAGTTTAAGAG+AGG 0.557983 7.2:+2357343 MS.gene22669:CDS
AGAGTGATGGTGGTGATGAG+AGG 0.569060 7.2:+2357414 MS.gene22669:CDS
GCGTAGTTTAAGAGAGGAAG+GGG 0.569207 7.2:+2357349 MS.gene22669:CDS
GAGTGATGGTGGTGATGAGA+GGG 0.569582 7.2:+2357415 MS.gene22669:CDS
ACATGAAGGAGAAGAAAACT+AGG 0.590117 7.2:+2358060 MS.gene22669:CDS
TGGCTTTGACGGAGAAGGAG+AGG 0.597092 7.2:+2357318 MS.gene22669:CDS
AATGAGGAAGAGAGTGATGG+TGG 0.598981 7.2:+2357404 MS.gene22669:CDS
TGTGGAGGCATTGAAGAATG+AGG 0.609129 7.2:+2357388 MS.gene22669:CDS
CATTGACAATGATAACATGA+AGG 0.609470 7.2:+2358046 MS.gene22669:CDS
ACAGGGTTCAAGTGCCGTTG+TGG 0.610080 7.2:+2358113 MS.gene22669:CDS
CAACCATTGGCACAAAGTGA+CGG 0.611416 7.2:-2357870 None:intergenic
AGAAGAACATACATGCAAAG+TGG 0.612995 7.2:+2358166 MS.gene22669:CDS
GGTGCGTAGGTTAAGACGTG+TGG 0.614414 7.2:+2357370 MS.gene22669:CDS
ACTAAGTGAAACAACATGCA+TGG 0.623449 7.2:+2357845 MS.gene22669:CDS
AAGAATGAGGAAGAGAGTGA+TGG 0.624967 7.2:+2357401 MS.gene22669:CDS
ATTGAATTGAAAATCAACAA+TGG 0.628122 7.2:-2357447 None:intergenic
GCTTTGACGGAGAAGGAGAG+GGG 0.637010 7.2:+2357320 MS.gene22669:CDS
GCGTAGGTTAAGACGTGTGG+AGG 0.638442 7.2:+2357373 MS.gene22669:CDS
ACAAAATCCTTTGTGTATGG+GGG 0.652043 7.2:+2358223 MS.gene22669:CDS
CACAAAATACATCACCACAA+CGG 0.670499 7.2:-2358127 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATTTGGTAAAAAAAAAAA+GGG - chr7.2:2357790-2357809 None:intergenic 10.0%
!! TATATTTGGTAAAAAAAAAA+AGG - chr7.2:2357791-2357810 None:intergenic 10.0%
!!! TATAATTTTCAATTGAATTT+TGG + chr7.2:2357642-2357661 MS.gene22669:intron 10.0%
!! GAAATAGTTGTAATTATATT+TGG - chr7.2:2357805-2357824 None:intergenic 15.0%
!! ATTGAATTGAAAATCAACAA+TGG - chr7.2:2357450-2357469 None:intergenic 20.0%
!! TAAACAAAAAATATAACCTG+CGG - chr7.2:2357595-2357614 None:intergenic 20.0%
!!! AAAAAGGGTATAGAATAATT+AGG - chr7.2:2357775-2357794 None:intergenic 20.0%
!!! GATTTTGTTTCTCTAATATT+TGG - chr7.2:2358211-2358230 None:intergenic 20.0%
! AAACAAAATCCTTTGTGTAT+GGG + chr7.2:2358221-2358240 MS.gene22669:CDS 25.0%
! CACAAATATCATTGATACTA+GGG - chr7.2:2358004-2358023 None:intergenic 25.0%
! TCACAAATATCATTGATACT+AGG - chr7.2:2358005-2358024 None:intergenic 25.0%
!! AAAGTGGATTTAAAAGAGAT+TGG + chr7.2:2358182-2358201 MS.gene22669:CDS 25.0%
AAACCTTATTCTTGAGTCAT+CGG + chr7.2:2357959-2357978 MS.gene22669:CDS 30.0%
AACAAAATCCTTTGTGTATG+GGG + chr7.2:2358222-2358241 MS.gene22669:CDS 30.0%
ACAAAAGAGATGAGATAGAT+AGG - chr7.2:2357521-2357540 None:intergenic 30.0%
AGCATATATCGTTTAGAAGT+TGG - chr7.2:2357720-2357739 None:intergenic 30.0%
CATTGACAATGATAACATGA+AGG + chr7.2:2358046-2358065 MS.gene22669:CDS 30.0%
CATTGTAACACTTTGAACAA+AGG - chr7.2:2357926-2357945 None:intergenic 30.0%
GAAACAAAATCCTTTGTGTA+TGG + chr7.2:2358220-2358239 MS.gene22669:CDS 30.0%
GATTCATTCACATTCATATG+CGG + chr7.2:2357740-2357759 MS.gene22669:intron 30.0%
! GATTATAGAACTGAAGTGTT+GGG - chr7.2:2357499-2357518 None:intergenic 30.0%
AAAAAATATAACCTGCGGTG+TGG - chr7.2:2357590-2357609 None:intergenic 35.0%
AACAAAAAAGTGGGACTAAC+AGG + chr7.2:2358095-2358114 MS.gene22669:CDS 35.0%
ACAAAAAAGTGGGACTAACA+GGG + chr7.2:2358096-2358115 MS.gene22669:CDS 35.0%
ACAAAATCCTTTGTGTATGG+GGG + chr7.2:2358223-2358242 MS.gene22669:CDS 35.0%
ACAATCCAACTTTGCTGAAA+AGG - chr7.2:2357674-2357693 None:intergenic 35.0%
ACATGAAGGAGAAGAAAACT+AGG + chr7.2:2358060-2358079 MS.gene22669:CDS 35.0%
ACTAAGTGAAACAACATGCA+TGG + chr7.2:2357845-2357864 MS.gene22669:CDS 35.0%
ACTTTGCATGTATGTTCTTC+TGG - chr7.2:2358167-2358186 None:intergenic 35.0%
AGAAGAACATACATGCAAAG+TGG + chr7.2:2358166-2358185 MS.gene22669:CDS 35.0%
AGGAGTTGCAACAAAAAAGT+GGG + chr7.2:2358086-2358105 MS.gene22669:CDS 35.0%
CACAAAATACATCACCACAA+CGG - chr7.2:2358130-2358149 None:intergenic 35.0%
CTTTGCATGTATGTTCTTCT+GGG - chr7.2:2358166-2358185 None:intergenic 35.0%
GGATTATAGAACTGAAGTGT+TGG - chr7.2:2357500-2357519 None:intergenic 35.0%
TCAGCAAAGTTGGATTGTAT+TGG + chr7.2:2357676-2357695 MS.gene22669:intron 35.0%
TTGATACTAGGGTTCTTTGA+AGG - chr7.2:2357993-2358012 None:intergenic 35.0%
! ATCGTTTAGAAGTTGGTTGT+TGG - chr7.2:2357713-2357732 None:intergenic 35.0%
! GAACAAAGGTTCTTGTTTGA+AGG - chr7.2:2357912-2357931 None:intergenic 35.0%
! GTTTTCCTTTTCAGCAAAGT+TGG + chr7.2:2357666-2357685 MS.gene22669:intron 35.0%
!! CTCATCTCTTTTGTTGTGTT+TGG + chr7.2:2357527-2357546 MS.gene22669:intron 35.0%
!! TCTCTTTTGTTGTGTTTGGT+TGG + chr7.2:2357531-2357550 MS.gene22669:intron 35.0%
AAGAATGAGGAAGAGAGTGA+TGG + chr7.2:2357401-2357420 MS.gene22669:CDS 40.0%
AAGCCGATGACTCAAGAATA+AGG - chr7.2:2357965-2357984 None:intergenic 40.0%
ACCATAGAAACCACAACCAT+TGG - chr7.2:2357886-2357905 None:intergenic 40.0%
CAGGAGTTGCAACAAAAAAG+TGG + chr7.2:2358085-2358104 MS.gene22669:CDS 40.0%
TGCGTAGTTTAAGAGAGGAA+GGG + chr7.2:2357348-2357367 MS.gene22669:CDS 40.0%
! TTTGGTGCGTAGTTTAAGAG+AGG + chr7.2:2357343-2357362 MS.gene22669:CDS 40.0%
!! CGTTGTGGTGATGTATTTTG+TGG + chr7.2:2358128-2358147 MS.gene22669:CDS 40.0%
AATGAGGAAGAGAGTGATGG+TGG + chr7.2:2357404-2357423 MS.gene22669:CDS 45.0%
AGGAGAAGAAAACTAGGTGC+AGG + chr7.2:2358066-2358085 MS.gene22669:CDS 45.0%
ATATAACCTGCGGTGTGGTT+TGG - chr7.2:2357585-2357604 None:intergenic 45.0%
CAACCATTGGCACAAAGTGA+CGG - chr7.2:2357873-2357892 None:intergenic 45.0%
CTTATCACCCCCATACACAA+AGG - chr7.2:2358233-2358252 None:intergenic 45.0%
GCCAATGGTTGTGGTTTCTA+TGG + chr7.2:2357882-2357901 MS.gene22669:CDS 45.0%
GCGTAGTTTAAGAGAGGAAG+GGG + chr7.2:2357349-2357368 MS.gene22669:CDS 45.0%
GTGCGTAGTTTAAGAGAGGA+AGG + chr7.2:2357347-2357366 MS.gene22669:CDS 45.0%
TCACTTTGTGCCAATGGTTG+TGG + chr7.2:2357873-2357892 MS.gene22669:CDS 45.0%
!! TGTGGAGGCATTGAAGAATG+AGG + chr7.2:2357388-2357407 MS.gene22669:CDS 45.0%
! GTTCCGTCACTTTGTGCCAA+TGG + chr7.2:2357867-2357886 MS.gene22669:CDS 50.0%
! TGGTGGTGATGAGAGGGATA+AGG + chr7.2:2357421-2357440 MS.gene22669:CDS 50.0%
!! AGAGTGATGGTGGTGATGAG+AGG + chr7.2:2357414-2357433 MS.gene22669:CDS 50.0%
!! GAGTGATGGTGGTGATGAGA+GGG + chr7.2:2357415-2357434 MS.gene22669:CDS 50.0%
ACCAAACCAAACCACACCGC+AGG + chr7.2:2357576-2357595 MS.gene22669:intron 55.0%
GCGTAGGTTAAGACGTGTGG+AGG + chr7.2:2357373-2357392 MS.gene22669:CDS 55.0%
GCTTTGACGGAGAAGGAGAG+GGG + chr7.2:2357320-2357339 MS.gene22669:CDS 55.0%
GGTGCGTAGGTTAAGACGTG+TGG + chr7.2:2357370-2357389 MS.gene22669:CDS 55.0%
! ACAGGGTTCAAGTGCCGTTG+TGG + chr7.2:2358113-2358132 MS.gene22669:CDS 55.0%
! GGCTTTGACGGAGAAGGAGA+GGG + chr7.2:2357319-2357338 MS.gene22669:CDS 55.0%
! TAAGAGAGGAAGGGGTGCGT+AGG + chr7.2:2357357-2357376 MS.gene22669:CDS 55.0%
!! ACCTGCGGTGTGGTTTGGTT+TGG - chr7.2:2357580-2357599 None:intergenic 55.0%
!! TGGCTTTGACGGAGAAGGAG+AGG + chr7.2:2357318-2357337 MS.gene22669:CDS 55.0%
GACGGAGAAGGAGAGGGGTT+TGG + chr7.2:2357325-2357344 MS.gene22669:CDS 60.0%


Chromosome Type Strat End Strand Name
chr7.2 gene 2357317 2358256 2357317 ID=MS.gene22669
chr7.2 mRNA 2357317 2358256 2357317 ID=MS.gene22669.t1;Parent=MS.gene22669
chr7.2 exon 2357317 2357469 2357317 ID=MS.gene22669.t1.exon1;Parent=MS.gene22669.t1
chr7.2 CDS 2357317 2357469 2357317 ID=cds.MS.gene22669.t1;Parent=MS.gene22669.t1
chr7.2 exon 2357840 2358256 2357840 ID=MS.gene22669.t1.exon2;Parent=MS.gene22669.t1
chr7.2 CDS 2357840 2358256 2357840 ID=cds.MS.gene22669.t1;Parent=MS.gene22669.t1
Gene Sequence

>MS.gene22669

ATGGCTTTGACGGAGAAGGAGAGGGGTTTGGTGCGTAGTTTAAGAGAGGAAGGGGTGCGTAGGTTAAGACGTGTGGAGGCATTGAAGAATGAGGAAGAGAGTGATGGTGGTGATGAGAGGGATAAGGATGCCATTGTTGATTTTCAATTCAATGTAATGTAAAAGTGATTCAAACTATACCCAACACTTCAGTTCTATAATCCTATCTATCTCATCTCTTTTGTTGTGTTTGGTTGGTGTTTAGATTTAGATTAGATTGACCAAACCAAACCACACCGCAGGTTATATTTTTTGTTTATTTTAGTATATTGCTTTTATCTTTTACTATAATTTTCAATTGAATTTTGGCGTTTTCCTTTTCAGCAAAGTTGGATTGTATTGGATGTTTCTTTTCCAACAACCAACTTCTAAACGATATATGCTGATTCATTCACATTCATATGCGGATTCATTCACCTAATTATTCTATACCCTTTTTTTTTTTACCAAATATAATTACAACTATTTCAATCTTTTTCTTTAGTCAAAACTAAGTGAAACAACATGCATGGTTCCGTCACTTTGTGCCAATGGTTGTGGTTTCTATGGTTCTCCTTCAAACAAGAACCTTTGTTCAAAGTGTTACAATGTTTATCTCAAAGAAAACCTTATTCTTGAGTCATCGGCTTCTTGTCCTTCAAAGAACCCTAGTATCAATGATATTTGTGATGCTGTGACTGCTATTTCTCTCATTGACAATGATAACATGAAGGAGAAGAAAACTAGGTGCAGGAGTTGCAACAAAAAAGTGGGACTAACAGGGTTCAAGTGCCGTTGTGGTGATGTATTTTGTGGAATGCATAGATACCCAGAAGAACATACATGCAAAGTGGATTTAAAAGAGATTGGTCGCCAAATATTAGAGAAACAAAATCCTTTGTGTATGGGGGTGATAAGTTAG

Protein sequence

>MS.gene22669.t1

MALTEKERGLVRSLREEGVRRLRRVEALKNEEESDGGDERDKDAIVDFQFNSKLSETTCMVPSLCANGCGFYGSPSNKNLCSKCYNVYLKENLILESSASCPSKNPSINDICDAVTAISLIDNDNMKEKKTRCRSCNKKVGLTGFKCRCGDVFCGMHRYPEEHTCKVDLKEIGRQILEKQNPLCMGVIS