AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene22951

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene22951.t1 MTR_7g108890 97.989 348 7 0 1 348 217 564 0.0 716
MS.gene22951.t1 MTR_1g054880 81.844 347 62 1 1 347 214 559 0.0 600
MS.gene22951.t1 MTR_1g088020 80.802 349 64 2 1 347 209 556 0.0 580
MS.gene22951.t1 MTR_1g088225 79.943 349 65 3 1 347 209 554 0.0 569
MS.gene22951.t1 MTR_7g108875 74.499 349 84 2 1 347 220 565 0.0 540
MS.gene22951.t1 MTR_7g108900 72.126 348 93 3 1 346 215 560 0.0 527
MS.gene22951.t1 MTR_0458s0030 83.019 265 45 0 83 347 51 315 3.52e-169 474
MS.gene22951.t1 MTR_1g088040 83.019 265 45 0 83 347 51 315 3.52e-169 474
MS.gene22951.t1 MTR_1g083520 65.569 334 111 2 16 346 196 528 8.36e-160 458
MS.gene22951.t1 MTR_8g042910 63.253 332 122 0 15 346 281 612 1.76e-153 445
MS.gene22951.t1 MTR_3g010770 60.961 333 130 0 15 347 244 576 1.33e-146 426
MS.gene22951.t1 MTR_4g025690 60.606 330 119 5 18 346 194 513 1.42e-138 403
MS.gene22951.t1 MTR_7g108905 57.267 344 141 3 4 346 218 556 3.04e-137 401
MS.gene22951.t1 MTR_0458s0010 61.318 349 95 6 1 347 209 519 5.92e-135 394
MS.gene22951.t1 MTR_8g104645 56.193 331 141 3 18 346 44 372 5.10e-132 382
MS.gene22951.t1 MTR_4g025730 59.697 330 121 6 18 346 191 509 5.83e-132 386
MS.gene22951.t1 MTR_8g104655 56.193 331 141 3 18 346 213 541 2.43e-130 383
MS.gene22951.t1 MTR_2g044810 55.556 315 137 1 32 346 223 534 3.74e-125 370
MS.gene22951.t1 MTR_8g023310 55.758 330 138 3 18 346 267 589 5.61e-124 369
MS.gene22951.t1 MTR_2g044880 52.924 342 153 3 6 346 191 525 1.62e-123 365
MS.gene22951.t1 MTR_4g087980 52.568 331 149 3 19 348 195 518 6.00e-123 363
MS.gene22951.t1 MTR_4g025670 54.167 312 142 1 36 346 178 489 1.05e-120 357
MS.gene22951.t1 MTR_4g124520 51.045 335 153 3 14 346 196 521 1.20e-120 358
MS.gene22951.t1 MTR_7g112580 54.313 313 130 3 35 346 242 542 1.04e-117 351
MS.gene22951.t1 MTR_4g035980 53.172 331 148 4 18 346 214 539 1.29e-117 351
MS.gene22951.t1 MTR_1g008140 51.149 348 163 4 1 346 265 607 2.36e-117 352
MS.gene22951.t1 MTR_7g050870 50.142 353 160 6 1 346 211 554 4.46e-116 347
MS.gene22951.t1 MTR_7g050980 51.652 333 151 5 18 346 229 555 1.38e-115 346
MS.gene22951.t1 MTR_4g087970 51.746 315 148 2 35 348 208 519 1.99e-115 344
MS.gene22951.t1 MTR_7g050950 50.904 332 154 4 18 346 228 553 2.13e-115 345
MS.gene22951.t1 MTR_8g042900 51.064 329 153 3 18 346 261 581 1.09e-114 345
MS.gene22951.t1 MTR_3g008720 51.351 333 155 4 16 346 200 527 2.04e-114 342
MS.gene22951.t1 MTR_3g033690 51.051 333 156 4 16 346 199 526 4.04e-114 341
MS.gene22951.t1 MTR_3g010780 50.760 329 153 3 18 346 255 574 5.15e-114 343
MS.gene22951.t1 MTR_3g008640 51.351 333 155 4 16 346 200 527 8.69e-114 340
MS.gene22951.t1 MTR_3g008700 51.051 333 156 4 16 346 200 527 2.33e-113 339
MS.gene22951.t1 MTR_3g091380 51.045 335 157 4 14 346 268 597 2.60e-113 342
MS.gene22951.t1 MTR_7g053360 51.051 333 156 4 16 346 200 527 4.60e-113 338
MS.gene22951.t1 MTR_4g124550 50.760 329 156 3 18 346 271 593 9.96e-112 337
MS.gene22951.t1 MTR_2g044810 56.349 252 110 0 95 346 214 465 2.81e-101 306
MS.gene22951.t1 MTR_1g086210 46.875 320 160 3 34 346 253 569 9.69e-101 308
MS.gene22951.t1 MTR_0618s0020 60.526 228 89 1 120 346 1 228 1.23e-100 296
MS.gene22951.t1 MTR_8g023150 48.649 333 149 7 18 342 260 578 1.73e-100 308
MS.gene22951.t1 MTR_8g104580 47.734 331 136 5 18 346 205 500 4.83e-100 304
MS.gene22951.t1 MTR_2g044660 49.521 313 151 3 34 346 327 632 7.64e-100 308
MS.gene22951.t1 MTR_8g093460 46.440 323 161 6 32 345 231 550 1.44e-97 300
MS.gene22951.t1 MTR_1g086190 44.985 329 177 2 18 346 239 563 3.76e-97 299
MS.gene22951.t1 MTR_1g083470 44.512 328 177 2 18 342 241 566 5.98e-96 296
MS.gene22951.t1 MTR_1g083500 44.512 328 177 2 18 342 241 566 5.98e-96 296
MS.gene22951.t1 MTR_7g112590 43.807 331 179 3 18 345 256 582 1.54e-94 293
MS.gene22951.t1 MTR_1g083510 44.277 332 174 4 18 345 258 582 9.46e-94 291
MS.gene22951.t1 MTR_4g005170 43.293 328 181 2 18 342 246 571 8.57e-93 290
MS.gene22951.t1 MTR_4g005180 43.293 328 181 2 18 342 228 553 9.10e-93 288
MS.gene22951.t1 MTR_1g083490 42.857 329 183 2 18 343 246 572 6.89e-92 286
MS.gene22951.t1 MTR_3g095920 45.253 316 160 6 40 345 251 563 5.03e-91 283
MS.gene22951.t1 MTR_7g110560 44.937 316 169 3 33 347 239 550 2.30e-90 281
MS.gene22951.t1 MTR_7g110490 44.144 333 176 4 18 346 240 566 1.10e-86 272
MS.gene22951.t1 MTR_7g110510 44.144 333 176 4 18 346 240 566 1.10e-86 272
MS.gene22951.t1 MTR_7g110440 43.243 333 179 4 18 346 240 566 4.18e-86 271
MS.gene22951.t1 MTR_5g083620 42.560 336 176 7 18 346 31 356 1.16e-84 260
MS.gene22951.t1 MTR_2g436040 41.390 331 180 4 18 342 220 542 7.93e-84 266
MS.gene22951.t1 MTR_4g120280 39.521 334 189 4 18 345 245 571 3.90e-82 261
MS.gene22951.t1 MTR_8g088610 40.979 327 183 5 18 342 54 372 1.39e-81 253
MS.gene22951.t1 MTR_4g120290 39.222 334 190 4 18 345 245 571 4.59e-80 255
MS.gene22951.t1 MTR_2g105560 41.587 315 166 6 34 346 41 339 9.29e-77 239
MS.gene22951.t1 MTR_7g107180 42.138 318 168 7 31 346 4 307 1.53e-75 235
MS.gene22951.t1 MTR_2g436050 39.432 317 182 3 35 345 251 563 7.56e-75 241
MS.gene22951.t1 MTR_7g110420 39.316 351 197 6 9 346 231 578 6.05e-74 239
MS.gene22951.t1 MTR_7g110430 36.578 339 174 4 11 348 236 534 3.38e-65 215
MS.gene22951.t1 MTR_7g111560 36.728 324 187 7 34 346 240 556 2.36e-62 208
MS.gene22951.t1 MTR_1g086540 35.207 338 186 9 13 335 25 344 9.47e-57 188
MS.gene22951.t1 MTR_6g024165 37.898 314 165 10 33 335 40 334 3.37e-56 186
MS.gene22951.t1 MTR_6g088810 38.033 305 161 7 42 335 90 377 6.47e-55 184
MS.gene22951.t1 MTR_6g012800 33.648 318 182 8 33 337 5 306 1.19e-53 179
MS.gene22951.t1 MTR_7g092780 33.974 312 182 6 34 335 90 387 2.92e-53 180
MS.gene22951.t1 MTR_8g102380 36.306 314 170 8 35 335 81 377 3.41e-52 177
MS.gene22951.t1 MTR_7g011530 33.648 318 182 9 33 337 5 306 5.37e-51 172
MS.gene22951.t1 MTR_6g042660 36.299 281 153 6 34 309 37 296 1.35e-50 171
MS.gene22951.t1 MTR_7g102600 30.499 341 207 6 11 335 67 393 2.35e-47 165
MS.gene22951.t1 MTR_1g033050 32.803 314 186 6 29 335 13 308 4.51e-47 162
MS.gene22951.t1 MTR_5g095490 33.981 309 173 8 40 335 67 357 2.62e-46 161
MS.gene22951.t1 MTR_5g083590 31.511 311 158 6 39 347 12 269 1.46e-45 157
MS.gene22951.t1 MTR_8g102380 38.224 259 132 7 35 280 81 324 2.51e-44 155
MS.gene22951.t1 MTR_2g033970 31.366 322 192 8 24 336 46 347 8.60e-44 154
MS.gene22951.t1 MTR_2g039530 36.774 310 151 12 18 313 40 318 1.00e-43 154
MS.gene22951.t1 MTR_4g124740 31.548 336 188 13 14 335 32 339 2.86e-43 153
MS.gene22951.t1 MTR_6g042690 35.315 286 154 9 34 309 39 303 5.47e-42 149
MS.gene22951.t1 MTR_5g093970 32.573 307 190 6 30 335 39 329 9.02e-42 149
MS.gene22951.t1 MTR_5g012600 30.312 320 194 7 31 338 60 362 2.19e-41 148
MS.gene22951.t1 MTR_5g088300 33.333 306 182 6 30 335 33 316 9.44e-41 145
MS.gene22951.t1 MTR_5g088290 33.225 307 182 7 30 335 44 328 5.23e-40 144
MS.gene22951.t1 MTR_2g436600 30.650 323 184 11 24 336 45 337 1.77e-38 140
MS.gene22951.t1 MTR_5g088280 32.573 307 183 7 30 335 35 318 3.43e-37 136
MS.gene22951.t1 MTR_4g051810 30.719 306 191 6 30 335 33 317 5.94e-37 135
MS.gene22951.t1 MTR_8g036225 29.969 327 189 11 32 344 31 331 2.17e-32 124
MS.gene22951.t1 MTR_2g073390 30.321 343 209 11 6 335 36 361 4.28e-32 124
MS.gene22951.t1 MTR_4g074290 29.524 315 191 9 31 335 68 361 5.25e-32 123
MS.gene22951.t1 MTR_0009s0350 30.172 348 203 12 6 335 36 361 1.07e-30 120
MS.gene22951.t1 MTR_0009s0340 30.172 348 203 12 6 335 36 361 1.07e-30 120
MS.gene22951.t1 MTR_6g025590 36.364 187 102 8 33 209 48 227 3.35e-25 102
MS.gene22951.t1 MTR_0408s0030 44.444 99 42 3 213 309 36 123 1.70e-17 79.0
MS.gene22951.t1 MTR_6g024200 31.977 172 96 6 165 335 125 276 7.30e-16 77.0
MS.gene22951.t1 MTR_4g130790 28.421 190 126 4 38 220 26 212 2.91e-14 72.4
MS.gene22951.t1 MTR_7g102580 43.023 86 39 2 111 191 73 153 5.74e-14 68.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene22951.t1 AT4G02330 68.768 349 104 2 1 347 227 572 4.97e-179 508
MS.gene22951.t1 AT2G47550 67.723 347 109 1 1 347 216 559 7.13e-174 495
MS.gene22951.t1 AT1G02810 66.097 351 115 1 1 347 228 578 2.00e-173 494
MS.gene22951.t1 AT3G10720 60.479 334 132 0 14 347 283 616 9.57e-150 436
MS.gene22951.t1 AT5G04970 58.084 334 140 0 14 347 288 621 1.29e-139 410
MS.gene22951.t1 AT2G45220 59.091 330 121 6 18 346 195 511 5.95e-133 389
MS.gene22951.t1 AT3G10720 63.077 260 96 0 88 347 1 260 2.27e-126 363
MS.gene22951.t1 AT1G11580 55.828 326 140 2 18 342 231 553 2.44e-126 374
MS.gene22951.t1 AT3G43270 55.589 331 140 4 18 347 202 526 6.08e-126 372
MS.gene22951.t1 AT1G11580 55.828 326 140 2 18 342 252 574 6.23e-126 373
MS.gene22951.t1 AT5G51490 53.274 336 147 4 14 346 208 536 8.57e-124 366
MS.gene22951.t1 AT3G60730 53.674 313 144 1 35 346 207 519 1.36e-123 365
MS.gene22951.t1 AT3G05620 56.090 312 125 2 35 346 242 541 2.72e-123 365
MS.gene22951.t1 AT1G23200 53.939 330 139 4 18 346 237 554 1.94e-122 363
MS.gene22951.t1 AT4G33220 54.217 332 144 3 18 348 80 404 7.06e-121 354
MS.gene22951.t1 AT2G26440 55.696 316 137 1 31 346 235 547 1.63e-120 358
MS.gene22951.t1 AT5G53370 53.433 335 149 4 14 346 256 585 1.01e-119 358
MS.gene22951.t1 AT4G33220 54.217 332 144 3 18 348 201 525 1.89e-119 355
MS.gene22951.t1 AT5G53370 53.433 335 149 4 14 346 292 621 4.06e-119 357
MS.gene22951.t1 AT5G51500 52.679 336 151 5 14 346 210 540 5.64e-118 352
MS.gene22951.t1 AT3G14310 54.242 330 143 3 18 346 270 592 1.39e-117 352
MS.gene22951.t1 AT3G49220 54.294 326 141 4 28 348 276 598 3.62e-116 349
MS.gene22951.t1 AT1G53830 54.878 328 136 4 18 342 265 583 1.14e-115 347
MS.gene22951.t1 AT3G49220 54.294 326 141 4 28 348 321 643 1.57e-115 348
MS.gene22951.t1 AT5G04960 50.760 329 150 5 16 342 242 560 1.47e-113 341
MS.gene22951.t1 AT4G00190 53.226 310 140 4 34 342 165 470 2.17e-113 338
MS.gene22951.t1 AT4G02300 48.555 346 172 3 2 346 192 532 1.23e-111 335
MS.gene22951.t1 AT3G10710 50.909 330 153 4 17 346 241 561 1.82e-111 336
MS.gene22951.t1 AT3G59010 52.266 331 141 5 18 347 215 529 3.79e-111 334
MS.gene22951.t1 AT4G02320 49.080 326 160 3 18 342 194 514 2.94e-110 331
MS.gene22951.t1 AT2G43050 50.453 331 150 4 18 347 200 517 4.12e-109 328
MS.gene22951.t1 AT2G26450 48.485 330 164 4 18 346 287 611 3.73e-108 329
MS.gene22951.t1 AT3G47400 50.147 341 164 4 9 346 257 594 3.46e-107 326
MS.gene22951.t1 AT3G05610 46.108 334 177 1 14 347 236 566 1.26e-106 327
MS.gene22951.t1 AT1G53840 49.080 326 150 6 18 340 269 581 4.08e-105 320
MS.gene22951.t1 AT5G27870 44.282 341 179 3 9 346 230 562 1.75e-102 317
MS.gene22951.t1 AT3G06830 44.444 351 172 3 12 342 217 564 6.82e-101 309
MS.gene22951.t1 AT4G33230 46.364 330 171 4 18 346 282 606 1.24e-99 306
MS.gene22951.t1 AT5G49180 47.588 311 159 2 34 343 260 567 1.19e-98 303
MS.gene22951.t1 AT5G20860 46.519 316 159 4 34 346 230 538 1.61e-98 301
MS.gene22951.t1 AT3G27980 47.134 314 160 3 34 346 189 497 2.02e-96 295
MS.gene22951.t1 AT4G03930 47.452 314 159 3 34 346 216 524 4.62e-96 295
MS.gene22951.t1 AT1G11590 47.452 314 159 3 34 346 216 524 1.13e-94 291
MS.gene22951.t1 AT3G14300 47.840 324 153 6 18 337 649 960 2.40e-94 301
MS.gene22951.t1 AT5G09760 47.134 314 153 6 30 334 237 546 3.40e-93 288
MS.gene22951.t1 AT5G09760 47.134 314 153 6 30 334 263 572 3.74e-93 289
MS.gene22951.t1 AT1G11370 50.000 258 126 1 20 277 32 286 1.33e-89 271
MS.gene22951.t1 AT4G15980 42.988 328 183 2 16 342 374 698 4.53e-87 276
MS.gene22951.t1 AT4G15980 42.988 328 183 2 16 342 384 708 7.36e-87 276
MS.gene22951.t1 AT5G64640 44.753 324 159 7 30 339 285 602 6.85e-85 268
MS.gene22951.t1 AT2G47030 41.587 315 177 4 35 346 278 588 2.72e-75 243
MS.gene22951.t1 AT5G20860 40.728 302 141 3 34 332 230 496 3.45e-75 241
MS.gene22951.t1 AT2G47040 39.692 325 189 4 25 346 275 595 2.47e-72 235
MS.gene22951.t1 AT3G62170 40.952 315 179 4 35 346 278 588 5.19e-72 234
MS.gene22951.t1 AT5G19730 35.987 314 173 7 33 335 85 381 5.22e-54 182
MS.gene22951.t1 AT2G36710 35.331 317 184 6 31 340 88 390 2.00e-53 181
MS.gene22951.t1 AT1G05310 35.577 312 177 6 34 335 91 388 2.90e-53 180
MS.gene22951.t1 AT3G17060 31.366 322 192 7 24 336 36 337 1.89e-52 177
MS.gene22951.t1 AT2G19150 32.515 326 190 8 27 342 34 339 3.27e-51 174
MS.gene22951.t1 AT2G21610 33.003 303 183 6 33 335 50 332 3.22e-50 171
MS.gene22951.t1 AT2G21610 33.003 303 183 6 33 335 55 337 4.09e-50 171
MS.gene22951.t1 AT3G29090 33.230 322 184 9 28 335 1 305 4.10e-50 170
MS.gene22951.t1 AT2G36700 31.494 308 191 5 33 335 41 333 8.75e-48 164
MS.gene22951.t1 AT5G18990 34.505 313 176 7 34 335 29 323 1.50e-47 164
MS.gene22951.t1 AT5G55590 32.704 318 185 8 30 335 78 378 2.34e-45 159
MS.gene22951.t1 AT3G24130 33.227 313 180 7 34 335 34 328 2.57e-44 155
MS.gene22951.t1 AT5G07430 33.540 322 187 9 23 335 52 355 1.34e-43 154
MS.gene22951.t1 AT5G26810 32.727 330 192 10 21 335 19 333 3.43e-43 152
MS.gene22951.t1 AT5G55590 34.091 264 147 7 30 281 78 326 3.49e-42 150
MS.gene22951.t1 AT2G47280 30.818 318 191 8 30 337 31 329 2.21e-41 147
MS.gene22951.t1 AT2G19150 33.333 267 150 7 27 283 34 282 6.99e-41 145
MS.gene22951.t1 AT5G47500 30.696 316 190 7 32 335 59 357 1.40e-40 146
MS.gene22951.t1 AT5G07420 31.776 321 194 8 23 335 52 355 1.53e-39 143
MS.gene22951.t1 AT5G61680 33.119 311 182 10 32 335 67 358 2.19e-38 140
MS.gene22951.t1 AT1G44980 40.984 183 105 1 18 200 62 241 3.98e-36 131
MS.gene22951.t1 AT1G69940 28.483 323 204 10 22 335 51 355 3.96e-35 132
MS.gene22951.t1 AT5G07410 28.829 333 208 11 14 335 41 355 4.63e-35 131
MS.gene22951.t1 AT3G49220 39.362 94 50 3 28 117 321 411 3.06e-11 64.7

Find 62 sgRNAs with CRISPR-Local

Find 117 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGAACAGTTGACTTCATATT+TGG 0.153800 7.3:+6011143 MS.gene22951:CDS
CTACTAGCAACCGTGTTATT+TGG 0.232079 7.3:-6009523 None:intergenic
TTCGAAGCTGCAGCTGTAAA+AGG 0.293834 7.3:-6011057 None:intergenic
TATCGCGAATGCGACATCTA+TGG 0.300622 7.3:+6011122 MS.gene22951:CDS
TATAAAAGCTAGTGATGATT+TGG 0.325850 7.3:+6011298 MS.gene22951:CDS
GTACTCAAGGACAGTTTATA+TGG 0.338879 7.3:+6011370 MS.gene22951:CDS
ATCCTTCTGGTTGGCGTGAA+TGG 0.350213 7.3:+6011417 MS.gene22951:CDS
GACAGTTTGATAAATCCTTC+TGG 0.365166 7.3:+6011404 MS.gene22951:CDS
TTGTCCACTGCTGGGAAGTC+GGG 0.371249 7.3:-6011204 None:intergenic
TAAAATTTCCACTTCCATCT+TGG 0.392759 7.3:-6009471 None:intergenic
AGCGTTGAATTGTCCACTGC+TGG 0.412036 7.3:-6011213 None:intergenic
GGTGATCATAATGTTATTGA+TGG 0.424519 7.3:+6009661 MS.gene22951:CDS
CGTTTCTTACCGCGACTGCT+TGG 0.433166 7.3:-6011019 None:intergenic
ATACTAGTATGAGAGTGAAT+TGG 0.446368 7.3:+6011504 MS.gene22951:CDS
ACGTTCAATTCAGCAACATT+TGG 0.449707 7.3:+6009691 MS.gene22951:CDS
AACAATTGATGAAAGTGTTA+CGG 0.454527 7.3:+6009426 MS.gene22951:CDS
GATGATTTGGCTCCGTTGAT+TGG 0.458849 7.3:+6011311 MS.gene22951:CDS
GCGACTGCTTGGTGTTTGCT+TGG 0.470992 7.3:-6011008 None:intergenic
GATAGTTGGTTGCCTCAAAC+TGG 0.484973 7.3:+6011593 MS.gene22951:CDS
TATGCAGAGTATAATAATAC+AGG 0.485066 7.3:+6011470 MS.gene22951:CDS
ATAACATTTCGCAACACGGC+TGG 0.488240 7.3:+6010984 MS.gene22951:CDS
ATTGTGATTGTTAACCAAGA+TGG 0.492084 7.3:+6009457 MS.gene22951:CDS
CTTGCAGCTGTGGTTGCACA+AGG 0.496740 7.3:+6010945 MS.gene22951:intron
AAGAAGTACTTGATGATGGT+TGG 0.510321 7.3:+6009610 MS.gene22951:CDS
CAAGCAGTCGCGGTAAGAAA+CGG 0.512615 7.3:+6011020 MS.gene22951:CDS
GTCCTTGAGTACTCCTTCCA+TGG 0.514153 7.3:-6011359 None:intergenic
ACTTGATGATGGTTGGAGAT+GGG 0.527173 7.3:+6009617 MS.gene22951:CDS
TACTTGATGATGGTTGGAGA+TGG 0.529077 7.3:+6009616 MS.gene22951:CDS
AGTATGAGAGTGAATTGGAT+TGG 0.532578 7.3:+6011509 MS.gene22951:CDS
AAACAAGAAGTACTTGATGA+TGG 0.536365 7.3:+6009606 MS.gene22951:CDS
ATTGTCCACTGCTGGGAAGT+CGG 0.542110 7.3:-6011205 None:intergenic
TAACATAACATTTCGCAACA+CGG 0.542157 7.3:+6010980 MS.gene22951:CDS
ATTAATCGAACGATTATCAC+TGG 0.547300 7.3:+6009640 MS.gene22951:CDS
AAGTATGAAACACCAGTTTG+AGG 0.552647 7.3:-6011605 None:intergenic
GAGTATAATAATACAGGACT+TGG 0.553401 7.3:+6011476 MS.gene22951:CDS
CAGTGAAAACATACCTTGGT+AGG 0.555300 7.3:+6011336 MS.gene22951:CDS
AAACATACCTTGGTAGGCCA+TGG 0.560177 7.3:+6011342 MS.gene22951:CDS
TGGATCTGTTCGACCTTGAG+CGG 0.562248 7.3:-6011240 None:intergenic
GTTTGATAAATCCTTCTGGT+TGG 0.562814 7.3:+6011408 MS.gene22951:CDS
GGTTGCACAAGGGTTCTTAG+CGG 0.564354 7.3:+6010956 MS.gene22951:CDS
ATTGTTAACCAAGATGGAAG+TGG 0.567528 7.3:+6009463 MS.gene22951:CDS
TTCGCGATAAAATTGTCTCA+TGG 0.571446 7.3:-6011108 None:intergenic
ACTGGTGTTTCATACTTGAG+TGG 0.576700 7.3:+6011611 MS.gene22951:CDS
GGCCATGGAAGGAGTACTCA+AGG 0.580211 7.3:+6011357 MS.gene22951:CDS
CTGGTTGGCGTGAATGGAGT+GGG 0.588896 7.3:+6011423 MS.gene22951:CDS
GCGTTGAATTGTCCACTGCT+GGG 0.593272 7.3:-6011212 None:intergenic
TGTCCACTGCTGGGAAGTCG+GGG 0.596925 7.3:-6011203 None:intergenic
AATAACACGGTTGCTAGTAG+TGG 0.598741 7.3:+6009526 MS.gene22951:CDS
TATCCCCGACTTCCCAGCAG+TGG 0.603880 7.3:+6011200 MS.gene22951:CDS
CTCCATTCACGCCAACCAGA+AGG 0.609653 7.3:-6011419 None:intergenic
TCTGGTTGGCGTGAATGGAG+TGG 0.611631 7.3:+6011422 MS.gene22951:CDS
ATACCTTGGTAGGCCATGGA+AGG 0.613409 7.3:+6011346 MS.gene22951:CDS
TTCAACGCTATAACCGCTCA+AGG 0.618255 7.3:+6011227 MS.gene22951:CDS
GGTACAGTGAAAACATACCT+TGG 0.633037 7.3:+6011332 MS.gene22951:CDS
GAAGCTGCAGCTGTAAAAGG+TGG 0.636578 7.3:-6011054 None:intergenic
GATTCAGCTAGAAATCGTCA+TGG 0.643888 7.3:+6009388 MS.gene22951:CDS
ACTCCTTCCATGGCCTACCA+AGG 0.644437 7.3:-6011349 None:intergenic
ATTCAGCTAGAAATCGTCAT+GGG 0.648742 7.3:+6009389 MS.gene22951:CDS
AAGCAAACACCAAGCAGTCG+CGG 0.652116 7.3:+6011010 MS.gene22951:CDS
ACAGATCCAAATCAAAACAC+AGG 0.659080 7.3:+6011254 MS.gene22951:CDS
TTGCAGCTGTGGTTGCACAA+GGG 0.674966 7.3:+6010946 MS.gene22951:intron
AAACGTTGCACCAAATAACA+CGG 0.679707 7.3:+6009513 MS.gene22951:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATTTTTTTTTTTATAATA+AGG - chr7.3:6010092-6010111 None:intergenic 0.0%
!! TATTATAAGGAAAAAAAAAT+AGG + chr7.3:6010053-6010072 MS.gene22951:intron 10.0%
!!! ATTTTTTTTTCCTTATAATA+CGG - chr7.3:6010053-6010072 None:intergenic 10.0%
!!! TCAAATAAACATTTTTTTTA+TGG + chr7.3:6010473-6010492 MS.gene22951:intron 10.0%
!!! TTAGTAAGTTTTTTTTATAA+GGG + chr7.3:6010652-6010671 MS.gene22951:intron 10.0%
!!! TTTAGTAAGTTTTTTTTATA+AGG + chr7.3:6010651-6010670 MS.gene22951:intron 10.0%
!! TTAATTTATCACATGTATTT+AGG + chr7.3:6009964-6009983 MS.gene22951:intron 15.0%
!!! TTTTTTTTATAAGGGATATT+TGG + chr7.3:6010660-6010679 MS.gene22951:intron 15.0%
!! TAAAGAAAATGACTTATGAA+TGG - chr7.3:6010759-6010778 None:intergenic 20.0%
!! TAATTCATGTTTAAGTAAAG+TGG - chr7.3:6009918-6009937 None:intergenic 20.0%
!! TATTAAAAATATACTCACTC+TGG - chr7.3:6010548-6010567 None:intergenic 20.0%
!!! AGTTTATATGGAATGTTTTA+TGG + chr7.3:6011382-6011401 MS.gene22951:CDS 20.0%
!!! GATTAAGTGTGATTTTATAA+AGG - chr7.3:6010784-6010803 None:intergenic 20.0%
!!! TCATGCATTTTATTTTTTGA+GGG - chr7.3:6010029-6010048 None:intergenic 20.0%
!!! TGCATACAACTTTTTTTTAT+TGG + chr7.3:6010690-6010709 MS.gene22951:intron 20.0%
!!! TTCATGCATTTTATTTTTTG+AGG - chr7.3:6010030-6010049 None:intergenic 20.0%
! AAGTATATATGATCATCTCT+TGG + chr7.3:6010622-6010641 MS.gene22951:intron 25.0%
! ATATCATAAAGTGAGCATAA+AGG - chr7.3:6010924-6010943 None:intergenic 25.0%
! GATTAAGTGTGATTGAATTT+AGG - chr7.3:6010737-6010756 None:intergenic 25.0%
! TATAAAAGCTAGTGATGATT+TGG + chr7.3:6011298-6011317 MS.gene22951:CDS 25.0%
! TATGCAGAGTATAATAATAC+AGG + chr7.3:6011470-6011489 MS.gene22951:CDS 25.0%
!! ATAATGCTAAAAACTTTTCC+TGG + chr7.3:6010175-6010194 MS.gene22951:intron 25.0%
!! TTTCTGATTTTTGTTACTGA+GGG + chr7.3:6009553-6009572 MS.gene22951:CDS 25.0%
!! TTTTACAGTGGATAACTTTT+TGG + chr7.3:6011565-6011584 MS.gene22951:CDS 25.0%
!! TTTTCAGTATTTCTTTTTCC+AGG - chr7.3:6010196-6010215 None:intergenic 25.0%
!!! AACAATTGATGAAAGTGTTA+CGG + chr7.3:6009426-6009445 MS.gene22951:CDS 25.0%
!!! ACTTTCAAAGTCTTGTTTAA+CGG - chr7.3:6010327-6010346 None:intergenic 25.0%
!!! ATCAAGTACTTCTTGTTTTT+GGG - chr7.3:6009604-6009623 None:intergenic 25.0%
AATGCATGAACCGTATTATA+AGG + chr7.3:6010040-6010059 MS.gene22951:intron 30.0%
ATACCAAGAAATACACTTTG+AGG - chr7.3:6010850-6010869 None:intergenic 30.0%
ATACTAGTATGAGAGTGAAT+TGG + chr7.3:6011504-6011523 MS.gene22951:CDS 30.0%
ATTAATCGAACGATTATCAC+TGG + chr7.3:6009640-6009659 MS.gene22951:CDS 30.0%
ATTGTGATTGTTAACCAAGA+TGG + chr7.3:6009457-6009476 MS.gene22951:CDS 30.0%
GAGTATAATAATACAGGACT+TGG + chr7.3:6011476-6011495 MS.gene22951:CDS 30.0%
GGTGATCATAATGTTATTGA+TGG + chr7.3:6009661-6009680 MS.gene22951:CDS 30.0%
TAAAATTTCCACTTCCATCT+TGG - chr7.3:6009474-6009493 None:intergenic 30.0%
TAACATAACATTTCGCAACA+CGG + chr7.3:6010980-6010999 MS.gene22951:CDS 30.0%
TTTCATGCATGCTATAAGTA+AGG - chr7.3:6009943-6009962 None:intergenic 30.0%
! CACATGTATTTAGGATTATG+TGG + chr7.3:6009973-6009992 MS.gene22951:intron 30.0%
! CTTTCTGATTTTTGTTACTG+AGG + chr7.3:6009552-6009571 MS.gene22951:CDS 30.0%
!! AAACAAGAAGTACTTGATGA+TGG + chr7.3:6009606-6009625 MS.gene22951:CDS 30.0%
!!! CATCAAGTACTTCTTGTTTT+TGG - chr7.3:6009605-6009624 None:intergenic 30.0%
!!! GCATTTTATTTTTTGAGGGA+GGG - chr7.3:6010025-6010044 None:intergenic 30.0%
!!! TGCATTTTATTTTTTGAGGG+AGG - chr7.3:6010026-6010045 None:intergenic 30.0%
AAACGTTGCACCAAATAACA+CGG + chr7.3:6009513-6009532 MS.gene22951:CDS 35.0%
AAATATACTCACTCTGGTCA+TGG - chr7.3:6010542-6010561 None:intergenic 35.0%
AAGTATGAAACACCAGTTTG+AGG - chr7.3:6011608-6011627 None:intergenic 35.0%
ACAGATCCAAATCAAAACAC+AGG + chr7.3:6011254-6011273 MS.gene22951:CDS 35.0%
ACGTTCAATTCAGCAACATT+TGG + chr7.3:6009691-6009710 MS.gene22951:CDS 35.0%
AGTATGAGAGTGAATTGGAT+TGG + chr7.3:6011509-6011528 MS.gene22951:CDS 35.0%
AGTTGTCATGATGTTGTAGT+CGG - chr7.3:6010259-6010278 None:intergenic 35.0%
ATTCAGCTAGAAATCGTCAT+GGG + chr7.3:6009389-6009408 MS.gene22951:CDS 35.0%
ATTGTTAACCAAGATGGAAG+TGG + chr7.3:6009463-6009482 MS.gene22951:CDS 35.0%
CTACCTCAAAGTGTATTTCT+TGG + chr7.3:6010844-6010863 MS.gene22951:intron 35.0%
GACAGTTTGATAAATCCTTC+TGG + chr7.3:6011404-6011423 MS.gene22951:CDS 35.0%
GTACTCAAGGACAGTTTATA+TGG + chr7.3:6011370-6011389 MS.gene22951:CDS 35.0%
GTTTGATAAATCCTTCTGGT+TGG + chr7.3:6011408-6011427 MS.gene22951:CDS 35.0%
TTATGATATACTTGCAGCTG+TGG + chr7.3:6010935-6010954 MS.gene22951:intron 35.0%
TTCGCGATAAAATTGTCTCA+TGG - chr7.3:6011111-6011130 None:intergenic 35.0%
TTGCAAGGTAGTAATCTAGA+TGG - chr7.3:6010806-6010825 None:intergenic 35.0%
! ACAGTGGATAACTTTTTGGA+TGG + chr7.3:6011569-6011588 MS.gene22951:CDS 35.0%
! AGTGGATAACTTTTTGGATG+GGG + chr7.3:6011571-6011590 MS.gene22951:CDS 35.0%
! CAGTGGATAACTTTTTGGAT+GGG + chr7.3:6011570-6011589 MS.gene22951:CDS 35.0%
! GGAACAGTTGACTTCATATT+TGG + chr7.3:6011143-6011162 MS.gene22951:CDS 35.0%
! GTTTTCACTGTACCAATCAA+CGG - chr7.3:6011326-6011345 None:intergenic 35.0%
! TCTAGATGGATGATTTTGCA+AGG - chr7.3:6010821-6010840 None:intergenic 35.0%
! TGATGCTGCTAATTTTACAG+TGG + chr7.3:6011553-6011572 MS.gene22951:CDS 35.0%
!! AAGAAGTACTTGATGATGGT+TGG + chr7.3:6009610-6009629 MS.gene22951:CDS 35.0%
!! CTTTGAGGTAGTAATCTAGA+TGG - chr7.3:6010835-6010854 None:intergenic 35.0%
!!! GAAGTTCCTGTGTTTTGATT+TGG - chr7.3:6011263-6011282 None:intergenic 35.0%
AATAACACGGTTGCTAGTAG+TGG + chr7.3:6009526-6009545 MS.gene22951:CDS 40.0%
ACTGGTGTTTCATACTTGAG+TGG + chr7.3:6011611-6011630 MS.gene22951:CDS 40.0%
CAGTGAAAACATACCTTGGT+AGG + chr7.3:6011336-6011355 MS.gene22951:CDS 40.0%
GATTCAGCTAGAAATCGTCA+TGG + chr7.3:6009388-6009407 MS.gene22951:CDS 40.0%
GGTACAGTGAAAACATACCT+TGG + chr7.3:6011332-6011351 MS.gene22951:CDS 40.0%
GTTTAACGGAAAACACGAGA+GGG - chr7.3:6010313-6010332 None:intergenic 40.0%
TGTTTAACGGAAAACACGAG+AGG - chr7.3:6010314-6010333 None:intergenic 40.0%
! ACTTTTTGGATGGGGATAGT+TGG + chr7.3:6011579-6011598 MS.gene22951:CDS 40.0%
! CTACTAGCAACCGTGTTATT+TGG - chr7.3:6009526-6009545 None:intergenic 40.0%
! TTGACTACTCTTTTGAACGC+TGG + chr7.3:6010347-6010366 MS.gene22951:intron 40.0%
!! ACTTGATGATGGTTGGAGAT+GGG + chr7.3:6009617-6009636 MS.gene22951:CDS 40.0%
!! TACTTGATGATGGTTGGAGA+TGG + chr7.3:6009616-6009635 MS.gene22951:CDS 40.0%
!!! GATGCTAGCTAGCTTTTGAT+TGG - chr7.3:6009837-6009856 None:intergenic 40.0%
AAACATACCTTGGTAGGCCA+TGG + chr7.3:6011342-6011361 MS.gene22951:CDS 45.0%
ATAACATTTCGCAACACGGC+TGG + chr7.3:6010984-6011003 MS.gene22951:CDS 45.0%
GATAGTTGGTTGCCTCAAAC+TGG + chr7.3:6011593-6011612 MS.gene22951:CDS 45.0%
TATCGCGAATGCGACATCTA+TGG + chr7.3:6011122-6011141 MS.gene22951:CDS 45.0%
TTCAACGCTATAACCGCTCA+AGG + chr7.3:6011227-6011246 MS.gene22951:CDS 45.0%
TTCGAAGCTGCAGCTGTAAA+AGG - chr7.3:6011060-6011079 None:intergenic 45.0%
! GATGATTTGGCTCCGTTGAT+TGG + chr7.3:6011311-6011330 MS.gene22951:CDS 45.0%
! TTTTACAGCTGCAGCTTCGA+AGG + chr7.3:6011059-6011078 MS.gene22951:CDS 45.0%
!! TATATAGTTAATATAAAATA+GGG + chr7.3:6010218-6010237 MS.gene22951:intron 5.0%
!! TTATATAGTTAATATAAAAT+AGG + chr7.3:6010217-6010236 MS.gene22951:intron 5.0%
AAGCAAACACCAAGCAGTCG+CGG + chr7.3:6011010-6011029 MS.gene22951:CDS 50.0%
ATACCTTGGTAGGCCATGGA+AGG + chr7.3:6011346-6011365 MS.gene22951:CDS 50.0%
ATTGTCCACTGCTGGGAAGT+CGG - chr7.3:6011208-6011227 None:intergenic 50.0%
CAAGCAGTCGCGGTAAGAAA+CGG + chr7.3:6011020-6011039 MS.gene22951:CDS 50.0%
GAAGCTGCAGCTGTAAAAGG+TGG - chr7.3:6011057-6011076 None:intergenic 50.0%
GCGTTGAATTGTCCACTGCT+GGG - chr7.3:6011215-6011234 None:intergenic 50.0%
GGTTGCACAAGGGTTCTTAG+CGG + chr7.3:6010956-6010975 MS.gene22951:CDS 50.0%
GTCCTTGAGTACTCCTTCCA+TGG - chr7.3:6011362-6011381 None:intergenic 50.0%
TCTAAGCGTACTCATGCGCA+TGG - chr7.3:6009808-6009827 None:intergenic 50.0%
TGGATCTGTTCGACCTTGAG+CGG - chr7.3:6011243-6011262 None:intergenic 50.0%
TTGCAGCTGTGGTTGCACAA+GGG + chr7.3:6010946-6010965 MS.gene22951:intron 50.0%
! AGCGTTGAATTGTCCACTGC+TGG - chr7.3:6011216-6011235 None:intergenic 50.0%
! ATCCTTCTGGTTGGCGTGAA+TGG + chr7.3:6011417-6011436 MS.gene22951:CDS 50.0%
ACTCCTTCCATGGCCTACCA+AGG - chr7.3:6011352-6011371 None:intergenic 55.0%
CGTTTCTTACCGCGACTGCT+TGG - chr7.3:6011022-6011041 None:intergenic 55.0%
CTCCATTCACGCCAACCAGA+AGG - chr7.3:6011422-6011441 None:intergenic 55.0%
CTGGTTGGCGTGAATGGAGT+GGG + chr7.3:6011423-6011442 MS.gene22951:CDS 55.0%
CTTGCAGCTGTGGTTGCACA+AGG + chr7.3:6010945-6010964 MS.gene22951:intron 55.0%
TCTGGTTGGCGTGAATGGAG+TGG + chr7.3:6011422-6011441 MS.gene22951:CDS 55.0%
TTGTCCACTGCTGGGAAGTC+GGG - chr7.3:6011207-6011226 None:intergenic 55.0%
!! GCGACTGCTTGGTGTTTGCT+TGG - chr7.3:6011011-6011030 None:intergenic 55.0%
!! GGCCATGGAAGGAGTACTCA+AGG + chr7.3:6011357-6011376 MS.gene22951:CDS 55.0%
TATCCCCGACTTCCCAGCAG+TGG + chr7.3:6011200-6011219 MS.gene22951:CDS 60.0%
TGTCCACTGCTGGGAAGTCG+GGG - chr7.3:6011206-6011225 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr7.3 gene 6009361 6011646 6009361 ID=MS.gene22951
chr7.3 mRNA 6009361 6011646 6009361 ID=MS.gene22951.t1;Parent=MS.gene22951
chr7.3 exon 6009361 6009712 6009361 ID=MS.gene22951.t1.exon1;Parent=MS.gene22951.t1
chr7.3 CDS 6009361 6009712 6009361 ID=cds.MS.gene22951.t1;Parent=MS.gene22951.t1
chr7.3 exon 6010952 6011646 6010952 ID=MS.gene22951.t1.exon2;Parent=MS.gene22951.t1
chr7.3 CDS 6010952 6011646 6010952 ID=cds.MS.gene22951.t1;Parent=MS.gene22951.t1
Gene Sequence

>MS.gene22951

ATGTCAGATAAAGTACGTGCTATTTATGATTCAGCTAGAAATCGTCATGGGCGAAAGTTACTTCAAACAATTGATGAAAGTGTTACGGTAAGTGAAATTGTGATTGTTAACCAAGATGGAAGTGGAAATTTTACAACTATTAATGATGCTATAAACGTTGCACCAAATAACACGGTTGCTAGTAGTGGTTACTTTCTGATTTTTGTTACTGAGGGTGTGTATCAAGAGTATGTATCTATACCCAAAAACAAGAAGTACTTGATGATGGTTGGAGATGGGATTAATCGAACGATTATCACTGGTGATCATAATGTTATTGATGGTTCTACAACGTTCAATTCAGCAACATTTGGTAAGCAAAACATTTGTTAATTTAGTTATACTCAACTCAAGTCAAGTATACGATAATTGATTGATGTCATTAATATTACAAGCAACACGTATCCATGCGCATGAGTACGCTTAGAGTTAAACCAATCAAAAGCTAGCTAGCATCAAAAGTCACGTTGAGTTTTTTAAAAACTAGTATTATCTTGACTAATTGATTCTCATAACCACTTTACTTAAACATGAATTATTCCTTACTTATAGCATGCATGAAAATTAATTTATCACATGTATTTAGGATTATGTGGTTATTGACAAATAATAAAATATATGACCCTCCCTCAAAAAATAAAATGCATGAACCGTATTATAAGGAAAAAAAAATAGGTGTATTTTGTTGTCCTTATTATAAAAAAAAAAATAAAGTCATTTGAGAGTGCATTAATTGCTAAAAAAAAACTATATAATTCTATTAAATGCAAGATATATAATGCTAAAAACTTTTCCTGGAAAAAGAAATACTGAAAACTTATATAGTTAATATAAAATAGGGAAATGCTCTTTCTCACCGACTACAACATCATGACAACTAATGTGTAAGATTATTCAAACTTCACATCTACCCTCTCGTGTTTTCCGTTAAACAAGACTTTGAAAGTTTGACTACTCTTTTGAACGCTGGCACGTATTGTTTTGTCAATCACGTTCGAGACATAGAACAGTTGAATATAAATATTTAATGATAGAGATGTATATATATGAAATAACATTTCAAATATATAATATCAAATAAACATTTTTTTTATGGATCATCAAAACTTCTTAGTCTATGTTATTTTTGTAATTGTTGACCATGACCAGAGTGAGTATATTTTTAATAGTGCTTGTTTTAATACTATTTTATTGTGATTTTCTATTTAATTTGATCAAATTCAAGTATATATGATCATCTCTTGGTCGATATTTAGTAAGTTTTTTTTATAAGGGATATTTGGTAGTTCATGCATACAACTTTTTTTTATTGGTTTAACGTTTCTCTATGCTAGCCTAAATTCAATCACACTTAATCCATTCATAAGTCATTTTCTTTACTCCTTTATAAAATCACACTTAATCCATCTAGATTACTACCTTGCAAAATCATCCATCTAGATTACTACCTCAAAGTGTATTTCTTGGTATAATGTGTAGTTTGAATTTGTTGTATAAATTTTGTACATGTGTTTGAATAATCCTTTATGCTCACTTTATGATATACTTGCAGCTGTGGTTGCACAAGGGTTCTTAGCGGTTAACATAACATTTCGCAACACGGCTGGACCAAGCAAACACCAAGCAGTCGCGGTAAGAAACGGAGCTGATTTGTCCACCTTTTACAGCTGCAGCTTCGAAGGCTATCAAGATACTTTGTACACACATTCCATGAGACAATTTTATCGCGAATGCGACATCTATGGAACAGTTGACTTCATATTTGGAAATGCTGCAGTTGTTTTACAATCTTGCAACATATATCCCCGACTTCCCAGCAGTGGACAATTCAACGCTATAACCGCTCAAGGTCGAACAGATCCAAATCAAAACACAGGAACTTCTATACATAATGCAACTATAAAAGCTAGTGATGATTTGGCTCCGTTGATTGGTACAGTGAAAACATACCTTGGTAGGCCATGGAAGGAGTACTCAAGGACAGTTTATATGGAATGTTTTATGGACAGTTTGATAAATCCTTCTGGTTGGCGTGAATGGAGTGGGAATTTTGCATTAAGCACATTATATTATGCAGAGTATAATAATACAGGACTTGGTTCAGATACTAGTATGAGAGTGAATTGGATTGGTTATCATCTTATTAATGCTACTGATGCTGCTAATTTTACAGTGGATAACTTTTTGGATGGGGATAGTTGGTTGCCTCAAACTGGTGTTTCATACTTGAGTGGATTGATCATATAG
Protein sequence

>MS.gene22951.t1

MSDKVRAIYDSARNRHGRKLLQTIDESVTVSEIVIVNQDGSGNFTTINDAINVAPNNTVASSGYFLIFVTEGVYQEYVSIPKNKKYLMMVGDGINRTIITGDHNVIDGSTTFNSATFAVVAQGFLAVNITFRNTAGPSKHQAVAVRNGADLSTFYSCSFEGYQDTLYTHSMRQFYRECDIYGTVDFIFGNAAVVLQSCNIYPRLPSSGQFNAITAQGRTDPNQNTGTSIHNATIKASDDLAPLIGTVKTYLGRPWKEYSRTVYMECFMDSLINPSGWREWSGNFALSTLYYAEYNNTGLGSDTSMRVNWIGYHLINATDAANFTVDNFLDGDSWLPQTGVSYLSGLII