Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23152.t1 | XP_003622766.3 | 97.6 | 506 | 12 | 0 | 1 | 506 | 4 | 509 | 3.20E-285 | 990.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23152.t1 | Q9Y2Z9 | 36.0 | 453 | 263 | 10 | 51 | 497 | 36 | 467 | 1.2e-67 | 258.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23152.t1 | G7IGT2 | 97.4 | 506 | 13 | 0 | 1 | 506 | 4 | 509 | 5.2e-285 | 989.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051903 | MS.gene23152 | -0.816324 | 5.86E-52 | -1.69E-46 |
| MS.gene052818 | MS.gene23152 | 0.805343 | 1.40E-49 | -1.69E-46 |
| MS.gene053340 | MS.gene23152 | -0.816543 | 5.24E-52 | -1.69E-46 |
| MS.gene05344 | MS.gene23152 | 0.819566 | 1.09E-52 | -1.69E-46 |
| MS.gene054403 | MS.gene23152 | -0.803017 | 4.26E-49 | -1.69E-46 |
| MS.gene054536 | MS.gene23152 | -0.802079 | 6.65E-49 | -1.69E-46 |
| MS.gene05469 | MS.gene23152 | -0.840414 | 8.82E-58 | -1.69E-46 |
| MS.gene056830 | MS.gene23152 | 0.823514 | 1.33E-53 | -1.69E-46 |
| MS.gene057999 | MS.gene23152 | -0.829901 | 3.97E-55 | -1.69E-46 |
| MS.gene058238 | MS.gene23152 | -0.816726 | 4.77E-52 | -1.69E-46 |
| MS.gene059872 | MS.gene23152 | 0.830245 | 3.27E-55 | -1.69E-46 |
| MS.gene06011 | MS.gene23152 | -0.806134 | 9.53E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 106 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAGCTTACTCTATTGATTT+TGG | 0.126562 | 7.2:+68458213 | MS.gene23152:CDS |
| GTGAATTCTGCTTTGGATTA+TGG | 0.153355 | 7.2:+68457256 | MS.gene23152:CDS |
| GATGCCAATCTTATTGCTTT+TGG | 0.176173 | 7.2:-68457382 | None:intergenic |
| GTCTGGGATTATACTGGTTT+TGG | 0.176180 | 7.2:+68455099 | MS.gene23152:CDS |
| GGAATAATTTGTCTAATAAT+TGG | 0.183693 | 7.2:-68449165 | None:intergenic |
| TGCTGGTCAAGGAGTTAATT+TGG | 0.203221 | 7.2:+68457504 | MS.gene23152:CDS |
| ATGGCTTTAGCTTGTTTCTT+AGG | 0.205020 | 7.2:+68449228 | MS.gene23152:CDS |
| TTTCTTTGTTCAGAAGAAAA+TGG | 0.221119 | 7.2:-68458337 | None:intergenic |
| TCTGCTTTGGATTATGGATA+TGG | 0.228052 | 7.2:+68457262 | MS.gene23152:CDS |
| AGCATCATTTCTTATGCTTC+AGG | 0.274061 | 7.2:+68458300 | MS.gene23152:CDS |
| CTGCAGGTCTGGGATTATAC+TGG | 0.275947 | 7.2:+68455093 | MS.gene23152:intron |
| TTGAGCAGGTGAAGGTTCTT+TGG | 0.279471 | 7.2:-68456538 | None:intergenic |
| TACCTTTATGCATGACAATA+AGG | 0.281079 | 7.2:-68455279 | None:intergenic |
| GCCATCATCGTAACGTTTGC+TGG | 0.302475 | 7.2:-68458171 | None:intergenic |
| GATGTAAATAAAGACTTTCT+AGG | 0.318553 | 7.2:+68455144 | MS.gene23152:CDS |
| ATTGCTTTGGGATCCGATAT+TGG | 0.321986 | 7.2:+68457571 | MS.gene23152:CDS |
| ACGAAGCGCGTGGTTCTAAT+AGG | 0.322509 | 7.2:+68457454 | MS.gene23152:CDS |
| TGGTGTTTCCTCTGTCTTTA+CGG | 0.333683 | 7.2:+68457413 | MS.gene23152:CDS |
| CAAGGAGTTAATTTGGGTTT+CGG | 0.334075 | 7.2:+68457511 | MS.gene23152:CDS |
| GCGCCTCGCAGAAAATTAAA+AGG | 0.337660 | 7.2:-68458237 | None:intergenic |
| GCTGGTCAAGGAGTTAATTT+GGG | 0.340643 | 7.2:+68457505 | MS.gene23152:CDS |
| TTCCTTATTGTCATGCATAA+AGG | 0.347498 | 7.2:+68455277 | MS.gene23152:CDS |
| CATCCTACATCAAGCTCATT+AGG | 0.347977 | 7.2:+68457289 | MS.gene23152:CDS |
| ACAATAGCAACATCATATTC+TGG | 0.357578 | 7.2:-68449186 | None:intergenic |
| TAGTGGCTTGTGTATCAAAA+AGG | 0.362497 | 7.2:+68451880 | MS.gene23152:CDS |
| TATTGCTGAGGGAATTGCTT+TGG | 0.368602 | 7.2:+68457558 | MS.gene23152:CDS |
| CTGCGAGGCGCAGCCTTCAA+TGG | 0.372497 | 7.2:+68458249 | MS.gene23152:CDS |
| ATTGCTGAGGGAATTGCTTT+GGG | 0.383849 | 7.2:+68457559 | MS.gene23152:CDS |
| GTTCTGAAGGTTGGTTCTGA+TGG | 0.386114 | 7.2:+68456989 | MS.gene23152:intron |
| ACGCTCAACTGCTTTGTCAT+TGG | 0.396956 | 7.2:-68451827 | None:intergenic |
| GCGCTTCGTTGCATAGGAAT+TGG | 0.399209 | 7.2:-68457440 | None:intergenic |
| ACGATGATGGCGATCCTTGA+TGG | 0.400212 | 7.2:+68458183 | MS.gene23152:CDS |
| CACACGATCCATCCTCTTGC+TGG | 0.407503 | 7.2:+68457487 | MS.gene23152:CDS |
| ATTGGAAGGTAGTGAATTAG+TGG | 0.410789 | 7.2:-68446835 | None:intergenic |
| GAAGGACGTGAATTCTGCTT+TGG | 0.412150 | 7.2:+68457249 | MS.gene23152:CDS |
| ATAACATACCTCTCCAATAT+CGG | 0.412612 | 7.2:-68457584 | None:intergenic |
| TTCTTTGTTCAGAAGAAAAT+GGG | 0.414915 | 7.2:-68458336 | None:intergenic |
| TTGCGGTCGTTTGACTCTGT+TGG | 0.416150 | 7.2:-68457202 | None:intergenic |
| GAATATGATGTTGCTATTGT+TGG | 0.416882 | 7.2:+68449189 | MS.gene23152:CDS |
| TACAAAGTTTGCTCCATTGA+AGG | 0.420380 | 7.2:-68458262 | None:intergenic |
| GTTTGACTCTGTTGGACTCA+TGG | 0.428215 | 7.2:-68457194 | None:intergenic |
| CCCATGGGTAGAAGTGCAAT+TGG | 0.435032 | 7.2:-68457145 | None:intergenic |
| TTAGCAGGATTCAAAACAAC+TGG | 0.435557 | 7.2:+68457034 | MS.gene23152:CDS |
| ATAGAATGTTGTCTTGAAAT+CGG | 0.437402 | 7.2:-68456448 | None:intergenic |
| GAAATGATGCTTCTCTTAAG+TGG | 0.455768 | 7.2:-68458288 | None:intergenic |
| TTTGTTAAAGAAGTACGAAC+AGG | 0.458218 | 7.2:+68458140 | MS.gene23152:CDS |
| GCATCATTTCTTATGCTTCA+GGG | 0.468534 | 7.2:+68458301 | MS.gene23152:CDS |
| TCCTCCAAAAGCAATAAGAT+TGG | 0.471896 | 7.2:+68457378 | MS.gene23152:CDS |
| TTTCTTCGGTTATTGATTTG+CGG | 0.477028 | 7.2:-68457219 | None:intergenic |
| ACCAGCAAACGTTACGATGA+TGG | 0.480196 | 7.2:+68458170 | MS.gene23152:CDS |
| TGGAGGAGGCATGGTTGGCA+TGG | 0.490427 | 7.2:+68449209 | MS.gene23152:CDS |
| ACTGTACTGACTCTAGGATC+AGG | 0.491607 | 7.2:-68451911 | None:intergenic |
| ATCCATCCTCTTGCTGGTCA+AGG | 0.497656 | 7.2:+68457493 | MS.gene23152:CDS |
| AACCACGCGCTTCGTTGCAT+AGG | 0.508714 | 7.2:-68457446 | None:intergenic |
| AGAAGTGCAATTGGACCGTT+AGG | 0.509627 | 7.2:-68457136 | None:intergenic |
| TCCAATTGCACTTCTACCCA+TGG | 0.512824 | 7.2:+68457144 | MS.gene23152:CDS |
| GCAGGTGAAGGTTCTTTGGA+TGG | 0.514528 | 7.2:-68456534 | None:intergenic |
| CACAAGCCACTACTCAAAGC+TGG | 0.517920 | 7.2:-68451869 | None:intergenic |
| TGGAGAAAAGATATAGAAGC+AGG | 0.519289 | 7.2:-68451938 | None:intergenic |
| CTTTCAAGAGTTATTGCTGA+GGG | 0.526008 | 7.2:+68457547 | MS.gene23152:CDS |
| TGCTATTGTTGGAGGAGGCA+TGG | 0.529145 | 7.2:+68449200 | MS.gene23152:CDS |
| ATTGTTGGAGGAGGCATGGT+TGG | 0.531811 | 7.2:+68449204 | MS.gene23152:CDS |
| ATGTAAATAAAGACTTTCTA+GGG | 0.534481 | 7.2:+68455145 | MS.gene23152:CDS |
| GGAGTTACTGTACTGACTCT+AGG | 0.536464 | 7.2:-68451917 | None:intergenic |
| CTCCTTGACCAGCAAGAGGA+TGG | 0.538413 | 7.2:-68457495 | None:intergenic |
| GTCATTGAAGTTAACTTCGA+CGG | 0.540040 | 7.2:-68456471 | None:intergenic |
| TTTGAAGCATGACCTTGAGC+AGG | 0.541441 | 7.2:-68456552 | None:intergenic |
| CTAACCATTGTGCATGGCAA+CGG | 0.543146 | 7.2:+68457107 | MS.gene23152:CDS |
| CATCATTTCTTATGCTTCAG+GGG | 0.547565 | 7.2:+68458302 | MS.gene23152:CDS |
| TCAAGGTCATGCTTCAAAGC+TGG | 0.548137 | 7.2:+68456557 | MS.gene23152:CDS |
| GCTCCTAATGAGCTTGATGT+AGG | 0.554345 | 7.2:-68457292 | None:intergenic |
| AAGATTGGCATCTGAAAGGA+TGG | 0.558524 | 7.2:+68457393 | MS.gene23152:CDS |
| TCACGCGTTAGAGAATTAGC+AGG | 0.558646 | 7.2:+68457019 | MS.gene23152:CDS |
| AAACTCTATGTCCGCTGTGG+AGG | 0.564790 | 7.2:+68456503 | MS.gene23152:CDS |
| AGCAATCCAGCTTTGAGTAG+TGG | 0.567117 | 7.2:+68451863 | MS.gene23152:CDS |
| CAGCAAGAGGATGGATCGTG+TGG | 0.568130 | 7.2:-68457486 | None:intergenic |
| CTGTACTGACTCTAGGATCA+GGG | 0.570834 | 7.2:-68451910 | None:intergenic |
| CAGTAGCATATATGCCAAGT+TGG | 0.571242 | 7.2:+68456593 | MS.gene23152:CDS |
| TATGATGTTGCTATTGTTGG+AGG | 0.576587 | 7.2:+68449192 | MS.gene23152:CDS |
| AAAGAACCTTCACCTGCTCA+AGG | 0.577643 | 7.2:+68456540 | MS.gene23152:CDS |
| AATGAGCTTGATGTAGGATG+AGG | 0.579179 | 7.2:-68457286 | None:intergenic |
| CAATAAGATTGGCATCTGAA+AGG | 0.586941 | 7.2:+68457389 | MS.gene23152:CDS |
| TAGCATATATGCCAAGTTGG+TGG | 0.591599 | 7.2:+68456596 | MS.gene23152:CDS |
| CAGGATTCAAAACAACTGGA+TGG | 0.592635 | 7.2:+68457038 | MS.gene23152:CDS |
| CTGAAGGTTGGTTCTGATGG+AGG | 0.595777 | 7.2:+68456992 | MS.gene23152:intron |
| GCATGACCTTGAGCAGGTGA+AGG | 0.595978 | 7.2:-68456546 | None:intergenic |
| GCTTTGCACTCAAAATTCCA+AGG | 0.602404 | 7.2:+68446775 | MS.gene23152:CDS |
| CCAATTGCACTTCTACCCAT+GGG | 0.614374 | 7.2:+68457145 | MS.gene23152:CDS |
| TTAACTCCTTGACCAGCAAG+AGG | 0.616124 | 7.2:-68457499 | None:intergenic |
| TCAAAGCTGGAACTTAGTGA+TGG | 0.623560 | 7.2:+68456570 | MS.gene23152:CDS |
| GATGTTGCTATTGTTGGAGG+AGG | 0.630273 | 7.2:+68449195 | MS.gene23152:CDS |
| TGGCAACGGTTTCTACCTAA+CGG | 0.637413 | 7.2:+68457121 | MS.gene23152:CDS |
| GTACTGACTCTAGGATCAGG+GGG | 0.641659 | 7.2:-68451908 | None:intergenic |
| TTTGGGATCCGATATTGGAG+AGG | 0.646712 | 7.2:+68457576 | MS.gene23152:CDS |
| CTTCTGCTAACCATTGTGCA+TGG | 0.647474 | 7.2:+68457101 | MS.gene23152:CDS |
| TACAAACTCTATGTCCGCTG+TGG | 0.649310 | 7.2:+68456500 | MS.gene23152:CDS |
| TCTTTCAAGAGTTATTGCTG+AGG | 0.650080 | 7.2:+68457546 | MS.gene23152:CDS |
| TAAAGAAGTACGAACAGGAG+AGG | 0.656176 | 7.2:+68458145 | MS.gene23152:CDS |
| TTCCTATGCAACGAAGCGCG+TGG | 0.662325 | 7.2:+68457444 | MS.gene23152:CDS |
| AATGACAAAGCAGTTGAGCG+TGG | 0.662725 | 7.2:+68451829 | MS.gene23152:CDS |
| GAAACCGTTGCCATGCACAA+TGG | 0.667101 | 7.2:-68457111 | None:intergenic |
| AGCAAGAGGATGGATCGTGT+GGG | 0.672228 | 7.2:-68457485 | None:intergenic |
| TGTACTGACTCTAGGATCAG+GGG | 0.673767 | 7.2:-68451909 | None:intergenic |
| TTGGCATGCCGTAAAGACAG+AGG | 0.692012 | 7.2:-68457421 | None:intergenic |
| TGGCGTATTCTCCTCCACAG+CGG | 0.763316 | 7.2:-68456514 | None:intergenic |
| TACTGACTCTAGGATCAGGG+GGG | 0.765501 | 7.2:-68451907 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.2 | gene | 68446749 | 68458350 | 68446749 | ID=MS.gene23152 |
| chr7.2 | mRNA | 68446749 | 68458350 | 68446749 | ID=MS.gene23152.t1;Parent=MS.gene23152 |
| chr7.2 | exon | 68446749 | 68446857 | 68446749 | ID=MS.gene23152.t1.exon1;Parent=MS.gene23152.t1 |
| chr7.2 | CDS | 68446749 | 68446857 | 68446749 | ID=cds.MS.gene23152.t1;Parent=MS.gene23152.t1 |
| chr7.2 | exon | 68449150 | 68449249 | 68449150 | ID=MS.gene23152.t1.exon2;Parent=MS.gene23152.t1 |
| chr7.2 | CDS | 68449150 | 68449249 | 68449150 | ID=cds.MS.gene23152.t1;Parent=MS.gene23152.t1 |
| chr7.2 | exon | 68451819 | 68451962 | 68451819 | ID=MS.gene23152.t1.exon3;Parent=MS.gene23152.t1 |
| chr7.2 | CDS | 68451819 | 68451962 | 68451819 | ID=cds.MS.gene23152.t1;Parent=MS.gene23152.t1 |
| chr7.2 | exon | 68454689 | 68454747 | 68454689 | ID=MS.gene23152.t1.exon4;Parent=MS.gene23152.t1 |
| chr7.2 | CDS | 68454689 | 68454747 | 68454689 | ID=cds.MS.gene23152.t1;Parent=MS.gene23152.t1 |
| chr7.2 | exon | 68455099 | 68455166 | 68455099 | ID=MS.gene23152.t1.exon5;Parent=MS.gene23152.t1 |
| chr7.2 | CDS | 68455099 | 68455166 | 68455099 | ID=cds.MS.gene23152.t1;Parent=MS.gene23152.t1 |
| chr7.2 | exon | 68455247 | 68455298 | 68455247 | ID=MS.gene23152.t1.exon6;Parent=MS.gene23152.t1 |
| chr7.2 | CDS | 68455247 | 68455298 | 68455247 | ID=cds.MS.gene23152.t1;Parent=MS.gene23152.t1 |
| chr7.2 | exon | 68456444 | 68456617 | 68456444 | ID=MS.gene23152.t1.exon7;Parent=MS.gene23152.t1 |
| chr7.2 | CDS | 68456444 | 68456617 | 68456444 | ID=cds.MS.gene23152.t1;Parent=MS.gene23152.t1 |
| chr7.2 | exon | 68456998 | 68457597 | 68456998 | ID=MS.gene23152.t1.exon8;Parent=MS.gene23152.t1 |
| chr7.2 | CDS | 68456998 | 68457597 | 68456998 | ID=cds.MS.gene23152.t1;Parent=MS.gene23152.t1 |
| chr7.2 | exon | 68458135 | 68458350 | 68458135 | ID=MS.gene23152.t1.exon9;Parent=MS.gene23152.t1 |
| chr7.2 | CDS | 68458135 | 68458350 | 68458135 | ID=cds.MS.gene23152.t1;Parent=MS.gene23152.t1 |
| Gene Sequence |
| Protein sequence |
