AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene23430

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene23430.t1 MTR_7g098430 98.184 826 15 0 1 826 1 826 0.0 1674
MS.gene23430.t1 MTR_8g088050 39.753 566 322 6 158 711 18 576 2.07e-134 429
MS.gene23430.t1 MTR_8g088050 39.753 566 322 6 158 711 18 576 2.10e-134 429
MS.gene23430.t1 MTR_8g088050 39.753 566 322 6 158 711 18 576 2.11e-134 429
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene23430.t1 AT1G04080 65.057 704 224 9 109 795 37 735 0.0 915
MS.gene23430.t1 AT1G04080 65.057 704 224 9 109 795 37 735 0.0 915
MS.gene23430.t1 AT1G04080 59.527 761 227 12 109 795 37 790 0.0 867
MS.gene23430.t1 AT1G04080 65.027 549 173 7 261 795 6 549 0.0 704
MS.gene23430.t1 AT1G04080 63.916 521 169 7 289 795 1 516 0.0 652
MS.gene23430.t1 AT5G46400 41.392 546 296 9 154 687 30 563 2.93e-128 410
MS.gene23430.t1 AT5G46400 40.000 325 188 4 364 687 25 343 2.42e-66 237
MS.gene23430.t1 AT5G46400 40.000 325 188 4 364 687 25 343 2.42e-66 237
MS.gene23430.t1 AT4G24270 25.392 319 185 14 162 465 59 339 8.68e-11 66.2
MS.gene23430.t1 AT4G24270 25.392 319 185 14 162 465 59 339 8.68e-11 66.2

Find 208 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGCTATGCATTTACTTATTC+TGG 0.134991 7.2:-12845215 MS.gene23430:CDS
GCTTCTCCTGCAGCCTCGGC+TGG 0.142915 7.2:+12845260 None:intergenic
TTATTCTGTCATTAGGCTTC+TGG 0.145350 7.2:-12839827 MS.gene23430:intron
TATCGATCCAATTGCTGATT+TGG 0.218895 7.2:+12842557 None:intergenic
CTTGCTACACCAGGGTTCTC+TGG 0.220827 7.2:+12839489 None:intergenic
ATTTGGATTCTCTAATATTC+TGG 0.228707 7.2:+12842574 None:intergenic
TGAGCTTGTAGTACAGAATT+AGG 0.232263 7.2:+12845164 None:intergenic
CAGAATTCCCTTTGTGTTAT+GGG 0.232733 7.2:-12843492 MS.gene23430:CDS
ATTATCTTTCGTTTCCTCTT+TGG 0.236191 7.2:-12842658 MS.gene23430:CDS
ACGTACGACCTCTTAATATT+GGG 0.271534 7.2:-12842077 MS.gene23430:CDS
TGAAAACTGGCATAATTATC+TGG 0.271539 7.2:-12842049 MS.gene23430:CDS
CTTATACATCTCTTCTCTAA+TGG 0.271968 7.2:+12842164 None:intergenic
CCAAACTGTTCTTGCCAAAC+AGG 0.276139 7.2:-12839272 MS.gene23430:CDS
GATACAGTTGGACTTGAAAA+TGG 0.279480 7.2:-12845014 MS.gene23430:CDS
CACGTACGACCTCTTAATAT+TGG 0.285155 7.2:-12842078 MS.gene23430:CDS
CTTCTCCTGCAGCCTCGGCT+GGG 0.286804 7.2:+12845261 None:intergenic
TTATCTTTCGTTTCCTCTTT+GGG 0.287270 7.2:-12842657 MS.gene23430:CDS
GCTGCTGCTCTGCCCAATTC+AGG 0.288615 7.2:-12845236 MS.gene23430:CDS
GCAGAATTCCCTTTGTGTTA+TGG 0.289100 7.2:-12843493 MS.gene23430:CDS
GCTGACCATTCATCTGTAAA+TGG 0.289348 7.2:-12845044 MS.gene23430:CDS
CGGCTGCAGGTTGCTGTGTC+GGG 0.291007 7.2:+12837898 None:intergenic
AATACAGCACTTGCAGGTTT+AGG 0.295664 7.2:+12842219 None:intergenic
CCTTGTGCTTGAGTCGTTGC+TGG 0.297776 7.2:+12839084 None:intergenic
AGTAAATGCATAGCCTGAAT+TGG 0.309418 7.2:+12845223 None:intergenic
GTATTCTCGATTTGTTTATT+TGG 0.319345 7.2:-12840270 MS.gene23430:intron
TGCTGTGTCGGGGCTGCAAC+AGG 0.320209 7.2:+12837909 None:intergenic
GTTCCACAAAGCACTGCTTA+TGG 0.321030 7.2:-12838451 MS.gene23430:CDS
TCCCTTTGTGTTATGGGTAT+TGG 0.321785 7.2:-12843486 MS.gene23430:CDS
TATTCTGTCATTAGGCTTCT+GGG 0.323007 7.2:-12839826 MS.gene23430:intron
TATGGGGCTTATGCTGGATA+TGG 0.331066 7.2:-12838508 MS.gene23430:CDS
TATCCAGTACTCGGGATAAT+TGG 0.333216 7.2:+12841463 None:intergenic
GCCGTTCCTCAGGCATATTA+TGG 0.342067 7.2:-12837879 MS.gene23430:CDS
GGCCATTGATTAGGTCCATT+TGG 0.344489 7.2:+12839135 None:intergenic
TGCCTTCTCTTTGTATGAAC+AGG 0.345610 7.2:-12840351 MS.gene23430:CDS
CCTAATCAATGGCCTAACTA+TGG 0.352116 7.2:-12839126 MS.gene23430:CDS
ATCGACCTCTTTCAGAATTA+CGG 0.355782 7.2:-12842338 MS.gene23430:CDS
TTCCACAAAGCACTGCTTAT+GGG 0.376637 7.2:-12838450 MS.gene23430:CDS
CCTGTTTGGCAAGAACAGTT+TGG 0.377400 7.2:+12839272 None:intergenic
TACACCAGGGTTCTCTGGGT+TGG 0.381425 7.2:+12839494 None:intergenic
TATGGAAGCTAGGGAAAGTA+TGG 0.382672 7.2:-12841426 MS.gene23430:CDS
TGCTGCTGGGTGTAGCCAGC+AGG 0.383369 7.2:+12839051 None:intergenic
TCTGTTAATACAGCACTTGC+AGG 0.384276 7.2:+12842213 None:intergenic
ATGAGTACATGCAGCAAGAC+TGG 0.385484 7.2:-12842619 MS.gene23430:CDS
CAATGTCAACTCGCTTTGGC+TGG 0.386893 7.2:+12839553 None:intergenic
AATAATCAGGCATGCCAATA+TGG 0.389365 7.2:-12840482 MS.gene23430:CDS
CCTGCAGCCTCGGCTGGGGC+AGG 0.390779 7.2:+12845266 None:intergenic
CCATAGTTAGGCCATTGATT+AGG 0.399945 7.2:+12839126 None:intergenic
GAGATGTTCAATCGATCAAA+AGG 0.399956 7.2:-12839311 MS.gene23430:CDS
AGCAGCTTCTCCTGCAGCCT+CGG 0.403142 7.2:+12845256 None:intergenic
GATGAAGCTGCTGCAGTGGC+AGG 0.405134 7.2:-12842309 MS.gene23430:CDS
ACACCAGGGTTCTCTGGGTT+GGG 0.411863 7.2:+12839495 None:intergenic
TGAGGCTGTACTCCATAGTT+AGG 0.412997 7.2:+12839114 None:intergenic
ATACAGGCTGCCGCTTCCTA+TGG 0.418623 7.2:-12838526 MS.gene23430:intron
AACTCCGACAAGCCCCTGTT+TGG 0.419402 7.2:+12839258 None:intergenic
TTCTCCTGCAGCCTCGGCTG+GGG 0.420645 7.2:+12845262 None:intergenic
TTGCGCTCGGTTCAAGGAGC+AGG 0.422648 7.2:-12840578 MS.gene23430:CDS
GGAGAATTATCTGCACCATC+AGG 0.426487 7.2:+12842240 None:intergenic
AATGTCAACTCGCTTTGGCT+GGG 0.427195 7.2:+12839554 None:intergenic
TATTACCTACGAACTGTGTC+TGG 0.428305 7.2:+12843322 None:intergenic
CATGATATTGCAGGGGAAGT+TGG 0.428308 7.2:-12840378 MS.gene23430:intron
ACGGCTGCAGGTTGCTGTGT+CGG 0.430705 7.2:+12837897 None:intergenic
TTCACCTGTGCAGGATATGC+AGG 0.431301 7.2:+12838418 None:intergenic
TTAGCACCAACCTTGGGAAC+AGG 0.431376 7.2:+12845293 None:intergenic
TAAATGGTGTTGCAGCTTCA+AGG 0.434451 7.2:-12842373 MS.gene23430:intron
TACAGGCTGCCGCTTCCTAT+GGG 0.435003 7.2:-12838525 MS.gene23430:intron
CCTGCCCCAGCCGAGGCTGC+AGG 0.435556 7.2:-12845266 MS.gene23430:CDS
GCATTTACTTATTCTGGTGA+TGG 0.435692 7.2:-12845209 MS.gene23430:CDS
TACCAATCGATGTTCCATAT+TGG 0.436137 7.2:+12840468 None:intergenic
GATGGGAATGCTTATACTGC+TGG 0.441620 7.2:-12845191 MS.gene23430:CDS
CCGTTCCTCAGGCATATTAT+GGG 0.443253 7.2:-12837878 MS.gene23430:CDS
AATTGGATCGATATTTCAAC+AGG 0.444059 7.2:-12842547 MS.gene23430:intron
AAAACCAATTTACCTGCTGC+TGG 0.444538 7.2:+12839037 None:intergenic
GTCTTCTTGAAGCAATAATC+AGG 0.448175 7.2:-12840495 MS.gene23430:CDS
AGAGAATCCAAATCAGCAAT+TGG 0.448924 7.2:-12842564 MS.gene23430:CDS
TTGCTACACCAGGGTTCTCT+GGG 0.452718 7.2:+12839490 None:intergenic
GTTCACACTGAAATTTCTCC+AGG 0.453659 7.2:-12840517 MS.gene23430:CDS
CATTGACTTTCTAGAGTCAC+TGG 0.456681 7.2:-12839535 MS.gene23430:CDS
GATATGCACCCACTACAGAT+TGG 0.457141 7.2:+12839157 None:intergenic
GCCCCATAAGCAGTGCTTTG+TGG 0.457630 7.2:+12838448 None:intergenic
ATATTGGGGAGCTTGAAAAC+TGG 0.459736 7.2:-12842062 MS.gene23430:CDS
GCTGACCATGAGGCGCGCCT+TGG 0.460933 7.2:-12843454 MS.gene23430:CDS
GCTTCCTATGGGGCTTATGC+TGG 0.462340 7.2:-12838514 MS.gene23430:CDS
TCTGGTTGGAAAGGAAGCTC+TGG 0.463748 7.2:-12845084 MS.gene23430:CDS
CTTGCCAAACAGGGGCTTGT+CGG 0.464209 7.2:-12839262 MS.gene23430:CDS
GAGTTCGATTCTAAGATCAT+TGG 0.466817 7.2:-12842132 MS.gene23430:CDS
CATTTACTTATTCTGGTGAT+GGG 0.468008 7.2:-12845208 MS.gene23430:CDS
CACTTCCATTTACAGATGAA+TGG 0.468952 7.2:+12845039 None:intergenic
TGCTGGCTACACCCAGCAGC+AGG 0.471209 7.2:-12839049 MS.gene23430:intron
TCGAATCCTGTTCCCAAGGT+TGG 0.472109 7.2:-12845299 MS.gene23430:CDS
AACGAGTCAAAGCAAGCTGC+TGG 0.475326 7.2:-12845128 MS.gene23430:CDS
TCAAGCTCCCCAATATTAAG+AGG 0.477060 7.2:+12842069 None:intergenic
GTTTATGAACGAGCTGTTCA+AGG 0.479117 7.2:-12843409 MS.gene23430:CDS
GAACGAGGATTAGCTTATGT+TGG 0.479715 7.2:-12842686 MS.gene23430:CDS
AGCTTCCTTTCCAACCAGAC+TGG 0.482155 7.2:+12845088 None:intergenic
TGCAAATAATGTTCTCGCTC+GGG 0.483808 7.2:-12841399 MS.gene23430:CDS
GTAAATGCATAGCCTGAATT+GGG 0.486099 7.2:+12845224 None:intergenic
ATGTCAACTCGCTTTGGCTG+GGG 0.492568 7.2:+12839555 None:intergenic
GTGCCAATTATCCCGAGTAC+TGG 0.494096 7.2:-12841466 MS.gene23430:CDS
AAGTCAATGTCAACTCGCTT+TGG 0.496185 7.2:+12839549 None:intergenic
CAAGGACAACAATGGCCTGC+TGG 0.496647 7.2:-12839066 MS.gene23430:CDS
GCCTAATGAGGATTCCAGTC+TGG 0.497327 7.2:-12845102 MS.gene23430:CDS
ATTAACAGAAGCAGAAGAGT+TGG 0.497386 7.2:-12842199 MS.gene23430:CDS
GGAACTGATGCAGGCAATTG+TGG 0.498199 7.2:+12838469 None:intergenic
TCCAATACCCATAACACAAA+GGG 0.499353 7.2:+12843485 None:intergenic
CAGGCCATTGCCATTGAGAA+AGG 0.499761 7.2:-12840332 MS.gene23430:CDS
TATGCCTGAGGAACGGCTGC+AGG 0.506298 7.2:+12837885 None:intergenic
GCAACCTGCAGCCGTTCCTC+AGG 0.509331 7.2:-12837889 MS.gene23430:CDS
AAGAGGTCGTACGTGAAAGT+AGG 0.516439 7.2:+12842086 None:intergenic
GGCTTCTGGGAATTCCGAAA+AGG 0.517187 7.2:-12839813 MS.gene23430:CDS
GGTACTGACCAAGGTGTTGA+AGG 0.517289 7.2:-12842273 MS.gene23430:CDS
GAGCAGGCTGGAGATATTGT+TGG 0.520258 7.2:-12840562 MS.gene23430:CDS
CAGAGCCAAGGCGCGCCTCA+TGG 0.522186 7.2:+12843449 None:intergenic
ATATGCACCCACTACAGATT+GGG 0.523107 7.2:+12839158 None:intergenic
CGAGACAGAGAAGGTGGCAG+AGG 0.524685 7.2:-12843982 MS.gene23430:intron
GGAACATTAGCACCAACCTT+GGG 0.526018 7.2:+12845287 None:intergenic
TGCCAATATGGAACATCGAT+TGG 0.531852 7.2:-12840470 MS.gene23430:intron
TTGCAAATAATGTTCTCGCT+CGG 0.532036 7.2:-12841400 MS.gene23430:CDS
TCCTCCTGCATATCCTGCAC+AGG 0.532285 7.2:-12838422 MS.gene23430:intron
CGCTTCGAATCCTGTTCCCA+AGG 0.532298 7.2:-12845303 MS.gene23430:CDS
GTGTTGCAGCTTCAAGGAGT+TGG 0.532516 7.2:-12842367 MS.gene23430:intron
GTCGTTGCTGGCCAAGTTTG+AGG 0.533522 7.2:+12839096 None:intergenic
ATGGAGTACAGCCTCAAACT+TGG 0.534256 7.2:-12839107 MS.gene23430:CDS
ATCCAAATGGACCTAATCAA+TGG 0.534735 7.2:-12839137 MS.gene23430:CDS
ATGGGAATGCTTATACTGCT+GGG 0.536210 7.2:-12845190 MS.gene23430:CDS
ACCAGACTGGAATCCTCATT+AGG 0.536266 7.2:+12845101 None:intergenic
AGGAACATTAGCACCAACCT+TGG 0.537288 7.2:+12845286 None:intergenic
AACATAGCGTATCCAGTACT+CGG 0.538429 7.2:+12841454 None:intergenic
TCTGTTGCACTTGCTACACC+AGG 0.538737 7.2:+12839480 None:intergenic
TCAATGTATTTATCCCAAAG+AGG 0.538798 7.2:+12842644 None:intergenic
ATATTCGCTATAAGCACCTA+TGG 0.541316 7.2:-12843349 MS.gene23430:CDS
GCTCGGTTCAAGGAGCAGGC+TGG 0.541555 7.2:-12840574 MS.gene23430:CDS
CAAACTGTTCTTGCCAAACA+GGG 0.544913 7.2:-12839271 MS.gene23430:CDS
AGCATAAGCCCCATAGGAAG+CGG 0.546523 7.2:+12838516 None:intergenic
GCTGTCCGTAATTCTGAAAG+AGG 0.548224 7.2:+12842333 None:intergenic
GGCTGCAGGTTGCTGTGTCG+GGG 0.548629 7.2:+12837899 None:intergenic
GTGGCAGGTGTTGTTTCAGA+GGG 0.550122 7.2:-12842294 MS.gene23430:CDS
AATGCATCAGAGAATGTTGA+TGG 0.550820 7.2:-12844990 MS.gene23430:CDS
TCCACAAAGCACTGCTTATG+GGG 0.553188 7.2:-12838449 MS.gene23430:CDS
AGTGGCAGGTGTTGTTTCAG+AGG 0.553746 7.2:-12842295 MS.gene23430:CDS
GTGCTTTGTGGAACTGATGC+AGG 0.555027 7.2:+12838460 None:intergenic
AAACAACTCACAGATGCTTA+CGG 0.557128 7.2:-12844954 MS.gene23430:intron
AGCTGATGAAGCTGCTGCAG+TGG 0.558284 7.2:-12842313 MS.gene23430:CDS
GGTGTAAATCATTGCAAGGC+GGG 0.558474 7.2:+12842595 None:intergenic
GGGGCTTATGCTGGATATGG+AGG 0.560147 7.2:-12838505 MS.gene23430:CDS
AACTGTGTCTGGATCTCCAT+AGG 0.560352 7.2:+12843333 None:intergenic
TTTATGAACGAGCTGTTCAA+GGG 0.561629 7.2:-12843408 MS.gene23430:CDS
ATTATTGCTTCAAGAAGACC+TGG 0.562102 7.2:+12840499 None:intergenic
AATGAGGATTCCAGTCTGGT+TGG 0.562814 7.2:-12845098 MS.gene23430:CDS
AGAGGTCGTACGTGAAAGTA+GGG 0.564513 7.2:+12842087 None:intergenic
GGAAGCTCTGGACTCTGCTA+TGG 0.564721 7.2:-12845072 MS.gene23430:CDS
ATCACCTGCCCAATCTGTAG+TGG 0.565221 7.2:-12839166 MS.gene23430:CDS
AGATGTTCAATCGATCAAAA+GGG 0.566435 7.2:-12839310 MS.gene23430:CDS
GTAGTGGGTGCATATCCAAA+TGG 0.568266 7.2:-12839150 MS.gene23430:CDS
GGATTCCAGTCTGGTTGGAA+AGG 0.569228 7.2:-12845093 MS.gene23430:CDS
TTTGATTGACGAGACAGAGA+AGG 0.570064 7.2:-12843991 MS.gene23430:CDS
AAACCAATTTACCTGCTGCT+GGG 0.571184 7.2:+12839038 None:intergenic
TCAATCGATCAAAAGGGTTG+AGG 0.572118 7.2:-12839304 MS.gene23430:CDS
GATATTGCAGGGGAAGTTGG+AGG 0.576699 7.2:-12840375 MS.gene23430:intron
TAATGTTCCTGCCCCAGCCG+AGG 0.579939 7.2:-12845273 MS.gene23430:CDS
AAGAGAAGGCGACTTGAGCA+AGG 0.580408 7.2:-12842016 MS.gene23430:intron
TGCAGGATATGCAGGAGGAT+AGG 0.581110 7.2:+12838426 None:intergenic
TTCTGGTGTAAATCATTGCA+AGG 0.581222 7.2:+12842591 None:intergenic
GAATACTGAGCAAACAACAT+TGG 0.582577 7.2:+12840287 None:intergenic
GAACTGATGCAGGCAATTGT+GGG 0.582647 7.2:+12838470 None:intergenic
AGCTGCTGGAGTGCCTAATG+AGG 0.586064 7.2:-12845114 MS.gene23430:CDS
TGCTGCTGAGACGTATCCAT+CGG 0.586175 7.2:-12845342 MS.gene23430:CDS
AATGGAAGTGTTGATACAGT+TGG 0.591174 7.2:-12845026 MS.gene23430:CDS
CCCATAATATGCCTGAGGAA+CGG 0.592953 7.2:+12837878 None:intergenic
TGAGTACATGCAGCAAGACT+GGG 0.596076 7.2:-12842618 MS.gene23430:CDS
CAACTTTATCAGCAGAGCCA+AGG 0.596207 7.2:+12843437 None:intergenic
CGTACGACCTCTTAATATTG+GGG 0.599762 7.2:-12842076 MS.gene23430:CDS
TTACATATTCAGTGGACATG+TGG 0.600677 7.2:-12843384 MS.gene23430:CDS
ATATCCAGCATAAGCCCCAT+AGG 0.601936 7.2:+12838510 None:intergenic
ACTGTCAAAGCCACTACTTG+AGG 0.604117 7.2:-12839753 MS.gene23430:intron
AGCAAGTGCAACAGAGCGCG+AGG 0.605392 7.2:-12839472 MS.gene23430:CDS
TCAAGGGGTTACATATTCAG+TGG 0.607137 7.2:-12843392 MS.gene23430:CDS
GCACCCACTACAGATTGGGC+AGG 0.607563 7.2:+12839162 None:intergenic
TCACCTGCCCAATCTGTAGT+GGG 0.612384 7.2:-12839165 MS.gene23430:CDS
TTCCAATACCCATAACACAA+AGG 0.612695 7.2:+12843484 None:intergenic
AACACAAAGGGAATTCTGCT+AGG 0.613157 7.2:+12843497 None:intergenic
TATGGGAGATAGTGAAACAA+TGG 0.615550 7.2:-12845378 MS.gene23430:CDS
TGACCAAGGTGTTGAAGGAG+AGG 0.619456 7.2:-12842268 MS.gene23430:CDS
GTGTTGTTGTAAGCATCCGA+TGG 0.622239 7.2:+12845326 None:intergenic
ACATAGCGTATCCAGTACTC+GGG 0.622860 7.2:+12841455 None:intergenic
ATCAGAGAATGTTGATGGGT+CGG 0.627186 7.2:-12844985 MS.gene23430:CDS
TTGAGACAAACCTCAAGTAG+TGG 0.627545 7.2:+12839743 None:intergenic
CTGCTGAAGAAGATCGATTG+TGG 0.627715 7.2:-12844058 MS.gene23430:CDS
GGCCTGTTCATACAAAGAGA+AGG 0.629259 7.2:+12840349 None:intergenic
GATTGACGAGACAGAGAAGG+TGG 0.630394 7.2:-12843988 MS.gene23430:CDS
ACAGGCTGCCGCTTCCTATG+GGG 0.637522 7.2:-12838524 MS.gene23430:intron
GTACTCGGGATAATTGGCAC+AGG 0.638207 7.2:+12841469 None:intergenic
CTCAAGCACAAGGACAACAA+TGG 0.638781 7.2:-12839074 MS.gene23430:CDS
GTTTCAGAGGGTACTGACCA+AGG 0.640522 7.2:-12842282 MS.gene23430:CDS
GAAGGAGAGGTTCATCCTGA+TGG 0.646457 7.2:-12842255 MS.gene23430:CDS
GAACCTCTCCTTCAACACCT+TGG 0.647061 7.2:+12842265 None:intergenic
ATGCATCAGAGAATGTTGAT+GGG 0.647426 7.2:-12844989 MS.gene23430:CDS
CTGTTGCACTTGCTACACCA+GGG 0.651331 7.2:+12839481 None:intergenic
ACCTGTGCAGGATATGCAGG+AGG 0.651957 7.2:+12838421 None:intergenic
AAACTGTTCTTGCCAAACAG+GGG 0.652207 7.2:-12839270 MS.gene23430:CDS
ACACCCAACCCAGAGAACCC+TGG 0.662263 7.2:-12839498 MS.gene23430:CDS
TTATGAACGAGCTGTTCAAG+GGG 0.665698 7.2:-12843407 MS.gene23430:CDS
CCAGCAACGACTCAAGCACA+AGG 0.666384 7.2:-12839084 MS.gene23430:CDS
GAGATCCAGACACAGTTCGT+AGG 0.679080 7.2:-12843327 MS.gene23430:intron
TGGGAATGCTTATACTGCTG+GGG 0.686552 7.2:-12845189 MS.gene23430:CDS
GAAGAAATATGCTGACCATG+AGG 0.691106 7.2:-12843464 MS.gene23430:CDS
TAATTCCCATAATATGCCTG+AGG 0.692350 7.2:+12837873 None:intergenic
TGGTGTAAATCATTGCAAGG+CGG 0.722048 7.2:+12842594 None:intergenic
CTTGGGAACAGGATTCGAAG+CGG 0.730245 7.2:+12845304 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
chr7.2 gene 12837866 12845399 12837866 ID=MS.gene23430
chr7.2 mRNA 12837866 12845399 12837866 ID=MS.gene23430.t1;Parent=MS.gene23430
chr7.2 exon 12844955 12845399 12844955 ID=MS.gene23430.t1.exon1;Parent=MS.gene23430.t1
chr7.2 CDS 12844955 12845399 12844955 ID=cds.MS.gene23430.t1;Parent=MS.gene23430.t1
chr7.2 exon 12843983 12844089 12843983 ID=MS.gene23430.t1.exon2;Parent=MS.gene23430.t1
chr7.2 CDS 12843983 12844089 12843983 ID=cds.MS.gene23430.t1;Parent=MS.gene23430.t1
chr7.2 exon 12843328 12843557 12843328 ID=MS.gene23430.t1.exon3;Parent=MS.gene23430.t1
chr7.2 CDS 12843328 12843557 12843328 ID=cds.MS.gene23430.t1;Parent=MS.gene23430.t1
chr7.2 exon 12842548 12842715 12842548 ID=MS.gene23430.t1.exon4;Parent=MS.gene23430.t1
chr7.2 CDS 12842548 12842715 12842548 ID=cds.MS.gene23430.t1;Parent=MS.gene23430.t1
chr7.2 exon 12842017 12842380 12842017 ID=MS.gene23430.t1.exon5;Parent=MS.gene23430.t1
chr7.2 CDS 12842017 12842380 12842017 ID=cds.MS.gene23430.t1;Parent=MS.gene23430.t1
chr7.2 exon 12841379 12841522 12841379 ID=MS.gene23430.t1.exon6;Parent=MS.gene23430.t1
chr7.2 CDS 12841379 12841522 12841379 ID=cds.MS.gene23430.t1;Parent=MS.gene23430.t1
chr7.2 exon 12840471 12840623 12840471 ID=MS.gene23430.t1.exon7;Parent=MS.gene23430.t1
chr7.2 CDS 12840471 12840623 12840471 ID=cds.MS.gene23430.t1;Parent=MS.gene23430.t1
chr7.2 exon 12840271 12840387 12840271 ID=MS.gene23430.t1.exon8;Parent=MS.gene23430.t1
chr7.2 CDS 12840271 12840387 12840271 ID=cds.MS.gene23430.t1;Parent=MS.gene23430.t1
chr7.2 exon 12839754 12839834 12839754 ID=MS.gene23430.t1.exon9;Parent=MS.gene23430.t1
chr7.2 CDS 12839754 12839834 12839754 ID=cds.MS.gene23430.t1;Parent=MS.gene23430.t1
chr7.2 exon 12839449 12839604 12839449 ID=MS.gene23430.t1.exon10;Parent=MS.gene23430.t1
chr7.2 CDS 12839449 12839604 12839449 ID=cds.MS.gene23430.t1;Parent=MS.gene23430.t1
chr7.2 exon 12839050 12839349 12839050 ID=MS.gene23430.t1.exon11;Parent=MS.gene23430.t1
chr7.2 CDS 12839050 12839349 12839050 ID=cds.MS.gene23430.t1;Parent=MS.gene23430.t1
chr7.2 exon 12838423 12838542 12838423 ID=MS.gene23430.t1.exon12;Parent=MS.gene23430.t1
chr7.2 CDS 12838423 12838542 12838423 ID=cds.MS.gene23430.t1;Parent=MS.gene23430.t1
chr7.2 exon 12837866 12837961 12837866 ID=MS.gene23430.t1.exon13;Parent=MS.gene23430.t1
chr7.2 CDS 12837866 12837961 12837866 ID=cds.MS.gene23430.t1;Parent=MS.gene23430.t1
Gene Sequence

>MS.gene23430

ATGGGAGATAGTGAAACAATGGTTGCAGAAGCATCTGCTGCTGAGACGTATCCATCGGATGCTTACAACAACACCGCTTCGAATCCTGTTCCCAAGGTTGGTGCTAATGTTCCTGCCCCAGCCGAGGCTGCAGGAGAAGCTGCTGCTGCTCTGCCCAATTCAGGCTATGCATTTACTTATTCTGGTGATGGGAATGCTTATACTGCTGGGGATCCTAATTCTGTACTACAAGCTCAATTTAGTGCAACAAACGAGTCAAAGCAAGCTGCTGGAGTGCCTAATGAGGATTCCAGTCTGGTTGGAAAGGAAGCTCTGGACTCTGCTATGGTCTCTGCTGACCATTCATCTGTAAATGGAAGTGTTGATACAGTTGGACTTGAAAATGGAAATGCATCAGAGAATGTTGATGGGTCGGCTGATGAGAAACAACTCACAGATGCTTACGGTATGTATATGCCTTCTCTGTCTGTTATGTTAGCATACTCTTCGGGAAAAGTGAAGTTATTTCGTTTTTATTGTTATTGAAGTTTTGTTAATAAAAAAATCGGAGTTCAATGTTAAGGGTATGTATAGAGCTTGCAAGTAATATAATTAACCAATGATGGAGTCAAGGACTTAAGATTCAAGTGATTTATTATTAACCAGCAAATAGTTCATTTAGGCCTTGTAGTTCATGTAAGCTTGTAAGTAATACAATTGCATATCAATGTTGAGAACTAATGAATTTTCACCAATATTCATGAATGGAAATAAGAAAATTTTAATCGCTCATAGTAACAATAGTGTTGTCAATTGTGGATAACGGATAAAACAGTAGCGGTTTGTTAATATTCCGCTACGCTATAGCGGTATAGTGGTTGCTGTTTAGCAACACTGAGTAAGAAGCTTTATTTTTTCATGCATTGGAGGTGGCGTTTAAGGCTACTTCCATTTGTTTTTGTCTCAAGTGACTTTGAAATTTTTCCAGGCCTGGTTTGAATTGGATCTTGACCATATTGATATAATAACTAGGTTTATTAGGTGTATAAATACGTTCATACATTTACATTTTTTTTCCATTTTATTGGTTTACCAGTGCAACAAGATATCATGACTTTGGTTTGTAATTTGATGATCACATATTCTAAGCCACTTCTGCGATTTTTGGATGTCTTCTATTTTACTGAGTTGGCTGATTCAGGCCTAAACATACACGTCTTATCTCCATACGTCTTGTTTTTAATATTATCTTGTTATTAATTATTCACAAGCATATTGCTGTTCAACCAAATTATGGTTTTGCTGCTAATCATTTATCTCACGTTTAACAGCTGCGTTGTCTGCTGAAGAAGATCGATTGTGGAATATTGTAACAGCTAATTCTTCTGATTTTACTGCTTGGACTTCTTTGATTGACGAGACAGAGAAGGTGGCAGAGGTATGATATGTTGACCCAATTTTTAAGACGTGAATTACATGTCGAGGAATCAAAATAAATGACTGATCTCGATGTTTTCATATTAGTTTGATATATGACATGCATGCATTATGATTCAGTATACTACGTGTTTAATGAGATGGATAATTTATATTGTTAAATACTTAAACCTGCATCTGTTTAACGGGGGTGTCTTGATTAAGAATGTCTATAATGTACACACAAGTTATGTGGGCGATTTCTCTGGTTCTGTCTGTCGGTATATTATTTTTTAACCTGCTAGTATATTATTTTTTCCTTTTTTGTCCCTTATTACGAGATACTTGTAGTTATAGGTTTAGTGGCAAACTAATGTGATGTATTTGATTTTATTATTGATTTATTTTATGACTCATGGTTCTCATATAATGATTTTTTTATTGAAGAACAATATTTTGAAGATGCGTAGAGTCTATGATGCTTTCCTAGCAGAATTCCCTTTGTGTTATGGGTATTGGAAGAAATATGCTGACCATGAGGCGCGCCTTGGCTCTGCTGATAAAGTTGTTGAAGTTTATGAACGAGCTGTTCAAGGGGTTACATATTCAGTGGACATGTGGTTGCATTATTGCATATTCGCTATAAGCACCTATGGAGATCCAGACACAGTTCGTAGGTAATAGAGGCTTATTTACATGTTACCTTATTATTTTCTATGATCGGATTTTCTATTTACTAGGCTCAGCTGTTTCTTGCTTGCTTTCTAACCTGATTTCAGAGACCGTGCAACAGTACCATCTCCTTTTTATGGCGGAAAAGCTTTGATGGGATTGCATGTCAGAATGTTTTCCATTCTGATGTTTAAATCCTTGGTTTTGATTCCACTATGTATAGTAATTGAAATTTTGGGTCCATTTTCCCCTATAAACTTTTCAATTTGATTTCAAACTTCATAGTTGTGTGGAAGGGTTCTATTTTCCCCCCCTTTCCTGTTGAAATGGTTAGCTGTTTTTATCTTTGACACTATGAGGTGTATCAATTTGCAAATTGCTGCTGGGAAGATCTCGTCTGGTTAAGGGCTAGATGGCCTCTATGACTGCTCCATTTCTCGGACATTGTATGATTTTTTGCAGGAAGGTAAATGCAGCGTTTGGATGTCTTATATGATGTTACGAGTGATTTGTTTGTTACCTTAGAACACTAGTGCTGCTGTTTCCCATACATGGATGTGCATCAGTAAATGGATGCTTTAAGTTACAGATTAATCAAGACTGCTTGCTTATTTCAGGCTTTTTGAACGAGGATTAGCTTATGTTGGAACAGATTATCTTTCGTTTCCTCTTTGGGATAAATACATTGAATATGAGTACATGCAGCAAGACTGGGCCCGCCTTGCAATGATTTACACCAGAATATTAGAGAATCCAAATCAGCAATTGGATCGATATTTCAACAGGTAATTACTTGTTTTAATTTAATATTATCATTCCACTTCATTTGTTTCCAAAAAAGAAACACAATTACTATTTTTTGTTTTATATTTCTACATAATATCCCCTTCTACTATCTCCTCTCTATTTTCCCCCGAACTAAATTACTTCAACGCTGTAAATGGTGTTGCAGCTTCAAGGAGTTGGCTAGTAATCGACCTCTTTCAGAATTACGGACAGCTGATGAAGCTGCTGCAGTGGCAGGTGTTGTTTCAGAGGGTACTGACCAAGGTGTTGAAGGAGAGGTTCATCCTGATGGTGCAGATAATTCTCCTAAACCTGCAAGTGCTGTATTAACAGAAGCAGAAGAGTTGGAGAAGTATATCGCCATTAGAGAAGAGATGTATAAGAAAGCTAAAGAGTTCGATTCTAAGATCATTGGTTTTGAAACAACTATCAGGAGGCCCTACTTTCACGTACGACCTCTTAATATTGGGGAGCTTGAAAACTGGCATAATTATCTGGATTTTATAGAAAGAGAAGGCGACTTGAGCAAGGTAACCAAACTGCTTTCTTATAAGTGCACATTCTGTTCTAGTTTTTCCTGTTATAGATTGACTTACAGTAAGTAATATCTGCTGTATCATATGTCTTATAAGTGCACATCCTATTCTGTTGGATGAAGAAACTTTTTAATCGGGAATTGAACTGAGACTAAAGCATGGGCCTACAAATTGGTTTCTTGAGGCTAAGTACTTCAAACCCTAATTTGTCAGTGGACATTCTAGGTTTGTCATGTGTTTGCAAAAGTTTATTTTTAAAAGTGTGAATGTTATGAATGCAGGTATCTTCTAATCTTCATAATCACCTGTCTCAGGCAAGGAGGGTCCCTCTTTTCATATACGAGGGGCCTCTCTGTCCCGCCACGGAGTATTTCTAATGCTTTGAATTTTCTTTTTATGCTTCTCTTTCTTGATTTTTTTGCTTGCATTTACATTGTTGGACAATTTTTATGATGTGTTCTTTTCTTGTTTTGTTGTTTGTGGATCAGATTGTCAAGTTGTATGAGAGATGTGTCATTGCCTGTGCCAATTATCCCGAGTACTGGATACGCTATGTTTTGTGTATGGAAGCTAGGGAAAGTATGGATCTTGCAAATAATGTTCTCGCTCGGGCGAGCCAAGTTTTTGTCAAGGTAATTTCTTCCAAATGTCAAAGCTGTAGTTTTGGCAGCCATTTGCTGCTTTTTAGTGTTGTATTCATCTGTCCTGTTTTTAAGCTAAGTGAATTGGCAAGAAACACACATATATAATGGACTACTAGCCATAGGATAATAGTTCATGAAACTTGATATAGTGTGTCAATACACCATAGGTTGGTTTTTTGTTTACCTGATTGTAAATGGTGAAGGGGCATCTTATATTTTTGAGCTTTATGTTTATATGATTTCTTTAGGGTAAATGAAAGTTCAATTGTCTTTTATTTCCCCATCAGGGCCTTCGTTTAGCCGAGAATAGAAATCTTATGTACACCGCGTTGCCAATGACCCATTTTGTATAAGTGAAGGCGTATAAATGATTTTTTTTTTTTTTTATATCGCCTGTCTTACACAAGACATATGATCCCTTTGAGGTTGTGACTAGCTAGTGATTCAATTACTCATTTCCTTTTCATGGAGGTATTGGTATTATGTCGTAGATCAGTTATCCTGAAAGCCTAAACTGCGAGGTCACATATACGAAGAGTATTTTCATATCACTGGCAAGCTTTTTGTTGTGGCCCATCAGTGAATGGTGTTTATTATATGTGCAATTTAGTCTTGATTTATAAAAAGCAATGTTCATGTATACTTTGTTTATAAGCATAATTAGCTCAATTGCATTTCTTGTGTGAGAGGCGTTTCCCTTTGTTTTGTTGCTGCTTTATCATTTTTTGCTTGTCATAATGCAGAGACAACCCGAGATACATCTTTTTTGCGCTCGGTTCAAGGAGCAGGCTGGAGATATTGTTGGCGCTAGAGCTGCGTATCAACTTGTTCACACTGAAATTTCTCCAGGTCTTCTTGAAGCAATAATCAGGCATGCCAATATGGAACATCGATTGGTATGCTGACTTACAAAAGCGTTAATAAACCGTGACTCAATTTTTGCTTAAATTTACAGCTTTTCCATTCCATGATATTGCAGGGGAAGTTGGAGGATGCCTTCTCTTTGTATGAACAGGCCATTGCCATTGAGAAAGGAAAAGAACATTCACAAACACTGCCAATGTTGTTTGCTCAGTATTCTCGATTTGTTTATTTGGTAGGTTTGGCAGCATATTTGTATTCTTCGCTTGTTATTCAGTCTTGTTTTGTTTCAGACATTTCCGCATTTGGCTATAGAACATGAAATAGGGACAGTTCTCTTAATTAACATTAGCTACTGTTTTTGTTGACCTGTCTGGATCTGGCCAAATGATGATTATTTCTGCGAGCCTCTCATTTATCCCAATAAATAGAGATGATTCTGGCTTTATATTATTTAACAATTTTTATATTATTTAACAATTTTCTAGCTTTATGCTTCAGTTGCTTTACTCGTTTGTGTGAAAATTGTCTAGTCTTGTTTTTGAAATATTCTTTCTCATTCTGTCATTAGGCTTCTGGGAATTATAGTTTTATGCTTCAGTTGCTATGCTTGTTTGTGTGAAAGTTGTCTTGTCTTGTTTTTGAAATATTCTTTCTTATTCTGTCATTAGGCTTCTGGGAATTCCGAAAAGGCTAGAGAGATTTTAGTTGGGGGACTTGAGAATGCTTCACTGTCAAAGCCACTACTTGAGGTTTGTCTCAACTTTCACTTTCTATTGATACTTAACTAATATTATGCACTTGGCAATTTTTCTCTTCGTCTTTCCAAATGTTTTTCATATCTAACGAGATTATTCAACTTCATCTCTAACGAGAGTATTTAACTTCATCTCTTACACAGGCTTTATTGCATTTTGAGGCTATTCAACCCCAGCCAAAGCGAGTTGACATTGACTTTCTAGAGTCACTGGTTGTAAAATTCATAACACCCAACCCAGAGAACCCTGGTGTAGCAAGTGCAACAGAGCGCGAGGAGCTTTCTAACATTTTTCTGGAGGTGATTTCTTATGAGGAGGATGTTCAATATTATATTTTATTAGTTTCCTTTAGTTAGCAGTATTATTAAGTTCTTACAAATTTTCTGTTGTTTTTTCAGTTTTTAAATCTCTTTGGAGATGTTCAATCGATCAAAAGGGTTGAGGATAGACATGCCAAACTGTTCTTGCCAAACAGGGGCTTGTCGGAGTTGAAAAAACGTCATGCAGAGGATTTCTTAGCCTCAGATAAAACAAAAGTGTCAAGAGCTTATTCTGCTCAATCACCTGCCCAATCTGTAGTGGGTGCATATCCAAATGGACCTAATCAATGGCCTAACTATGGAGTACAGCCTCAAACTTGGCCAGCAACGACTCAAGCACAAGGACAACAATGGCCTGCTGGCTACACCCAGCAGCAGGTAAATTGGTTTTTGCCCTCAACTATTTTTCTTGTGTGTTGCTTCCCTAGTTCAAATCCCAGTGTTGCATGGTGTTTGTCTATGACGTGGGCGAAATTGTTTTATAAACTAGTGAGTCTTTCTTGCCGGGTTGTGCAATATAAACTTTTTTTTGTTTTTGGAAATAGTGGTGTACACAGGTGTGTCATAACCTTAATCCATGAAGCATTATAATTTGAAAACACCATATTAGCCTTGTTGAAATGGAATTTGCGCCATATCTCATTGTAATGAGTTGATTACCCTTTTAGTTTATATTTGCCTTGAACCCATAAAGACATCTTCATGCATTTTTATCTACAGAAAATCATGTCATAAATGCTCTCCATTCATGCTATGCCCAACAGTTTCATGTTTTCTAGAGTTAAACATTTGTCGCACTTGTAGTGTATGTTTGACACTGTGTTTGTGCATTCTAGAATGGTGAGGTATTCCTGTTCTGAAATTATCCATTCTGATTGAATACAGGCTGCCGCTTCCTATGGGGCTTATGCTGGATATGGAGGCAACTATGCTAACCCACAATTGCCTGCATCAGTTCCACAAAGCACTGCTTATGGGGCCTATCCTCCTGCATATCCTGCACAGGTGAAAAGCTGTTTCAGCCCTTATGATTTTTGCAGATTATTGTTTGCCTGATATGTTTCAGCTCTATAGTTCTTTCTTGCGAGTGTTAATTACAATTTTGCTCTGTAGAACAAGAGAAAGCAAAAAAAGTAAGGGGAATCATGTGATGGTGTTTGTGTGTGTGTATAGCAAGCAAAAAGAATGTAAAATATTATCAAACATACCATTGTTTTATAAGGCCTTGGCTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTGAGGGAACACGTGCCCAGCGTATCAGGATTTTCATTGAGTTGAGTTTCACCAGTTTAATAATTGCACTAATAGTACATAAGTTTGGTTTCATTTCTCAGTTGTATTTGACTTCTCTGACCTTTTGTTACTCTTTCCATTTCTATATTTCTGCTAAACCTGAGTACTGCGAATTAGATTAGTGTTGATTATTTTTTCTGAATTTCAGCCGGCACTGCCTCAACAAAATTATGCACAGCCTGTTGCAGCCCCGACACAGCAACCTGCAGCCGTTCCTCAGGCATATTATGGGAATTACTACTGA
Protein sequence

>MS.gene23430.t1

MGDSETMVAEASAAETYPSDAYNNTASNPVPKVGANVPAPAEAAGEAAAALPNSGYAFTYSGDGNAYTAGDPNSVLQAQFSATNESKQAAGVPNEDSSLVGKEALDSAMVSADHSSVNGSVDTVGLENGNASENVDGSADEKQLTDAYAALSAEEDRLWNIVTANSSDFTAWTSLIDETEKVAENNILKMRRVYDAFLAEFPLCYGYWKKYADHEARLGSADKVVEVYERAVQGVTYSVDMWLHYCIFAISTYGDPDTVRRLFERGLAYVGTDYLSFPLWDKYIEYEYMQQDWARLAMIYTRILENPNQQLDRYFNSFKELASNRPLSELRTADEAAAVAGVVSEGTDQGVEGEVHPDGADNSPKPASAVLTEAEELEKYIAIREEMYKKAKEFDSKIIGFETTIRRPYFHVRPLNIGELENWHNYLDFIEREGDLSKIVKLYERCVIACANYPEYWIRYVLCMEARESMDLANNVLARASQVFVKRQPEIHLFCARFKEQAGDIVGARAAYQLVHTEISPGLLEAIIRHANMEHRLGKLEDAFSLYEQAIAIEKGKEHSQTLPMLFAQYSRFVYLASGNSEKAREILVGGLENASLSKPLLEALLHFEAIQPQPKRVDIDFLESLVVKFITPNPENPGVASATEREELSNIFLEFLNLFGDVQSIKRVEDRHAKLFLPNRGLSELKKRHAEDFLASDKTKVSRAYSAQSPAQSVVGAYPNGPNQWPNYGVQPQTWPATTQAQGQQWPAGYTQQQAAASYGAYAGYGGNYANPQLPASVPQSTAYGAYPPAYPAQPALPQQNYAQPVAAPTQQPAAVPQAYYGNYY