Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23849.t1 | XP_003629046.2 | 95.7 | 232 | 10 | 0 | 1 | 232 | 53 | 284 | 6.00E-130 | 473.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23849.t1 | K7K424 | 58.2 | 232 | 97 | 0 | 1 | 232 | 65 | 296 | 2.3e-85 | 316.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23849.t1 | G7L7X0 | 95.7 | 232 | 10 | 0 | 1 | 232 | 53 | 284 | 4.4e-130 | 473.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050020 | MS.gene23849 | 0.846252 | 2.44E-59 | -1.69E-46 |
| MS.gene05016 | MS.gene23849 | 0.816176 | 6.33E-52 | -1.69E-46 |
| MS.gene050206 | MS.gene23849 | 0.814607 | 1.41E-51 | -1.69E-46 |
| MS.gene050807 | MS.gene23849 | 0.831787 | 1.37E-55 | -1.69E-46 |
| MS.gene050808 | MS.gene23849 | 0.800687 | 1.28E-48 | -1.69E-46 |
| MS.gene051789 | MS.gene23849 | 0.84034 | 9.23E-58 | -1.69E-46 |
| MS.gene053074 | MS.gene23849 | 0.832546 | 8.89E-56 | -1.69E-46 |
| MS.gene053383 | MS.gene23849 | 0.811711 | 6.11E-51 | -1.69E-46 |
| MS.gene053563 | MS.gene23849 | 0.804412 | 2.19E-49 | -1.69E-46 |
| MS.gene05456 | MS.gene23849 | 0.837398 | 5.32E-57 | -1.69E-46 |
| MS.gene055358 | MS.gene23849 | 0.81239 | 4.34E-51 | -1.69E-46 |
| MS.gene055359 | MS.gene23849 | 0.810433 | 1.16E-50 | -1.69E-46 |
| MS.gene05604 | MS.gene23849 | 0.800374 | 1.49E-48 | -1.69E-46 |
| MS.gene058168 | MS.gene23849 | 0.804396 | 2.20E-49 | -1.69E-46 |
| MS.gene059006 | MS.gene23849 | 0.847258 | 1.30E-59 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 51 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGATCTTTCCATTAATATTC+TGG | 0.193871 | 8.4:-31607604 | MS.gene23849:CDS |
| TGTGGATAAGAACAGTTTAT+TGG | 0.240059 | 8.4:-31625145 | MS.gene23849:CDS |
| GTCCATATTTGTCTTAAATA+TGG | 0.246667 | 8.4:+31608577 | None:intergenic |
| TTAATATTCTGGGGAAAATT+CGG | 0.275511 | 8.4:-31607593 | MS.gene23849:CDS |
| TTCCATCTTAATCAACCTTA+TGG | 0.288730 | 8.4:-31624876 | MS.gene23849:intron |
| TATAAGTGGTGGAGAGCTTC+TGG | 0.337143 | 8.4:-31607659 | MS.gene23849:CDS |
| TACCATATTTAAGACAAATA+TGG | 0.339295 | 8.4:-31608579 | MS.gene23849:CDS |
| ACCTGCTCTGATGTTGGTTC+TGG | 0.359832 | 8.4:+31606401 | None:intergenic |
| TAATATTCTGGGGAAAATTC+GGG | 0.361300 | 8.4:-31607592 | MS.gene23849:intron |
| GTGGATAAGAACAGTTTATT+GGG | 0.365082 | 8.4:-31625144 | MS.gene23849:CDS |
| GATCTTTCCATTAATATTCT+GGG | 0.370352 | 8.4:-31607603 | MS.gene23849:CDS |
| CCCCATCACACTTCACTTGG+AGG | 0.407649 | 8.4:-31624910 | MS.gene23849:CDS |
| AGACAAATATGGACATGGTT+AGG | 0.408270 | 8.4:-31608568 | MS.gene23849:CDS |
| TTAATTTCTTTGTTGGTAGC+TGG | 0.420608 | 8.4:-31608520 | MS.gene23849:CDS |
| AACCATAAGGTTGATTAAGA+TGG | 0.439395 | 8.4:+31624874 | None:intergenic |
| GGTGGTGGGTAAACCAATTC+AGG | 0.442053 | 8.4:-31606435 | MS.gene23849:CDS |
| TGAAGTGCAAATATGCCAAA+TGG | 0.444827 | 8.4:+31609348 | None:intergenic |
| ACCTGACCAAAGCAGAAAAC+CGG | 0.468692 | 8.4:+31608199 | None:intergenic |
| ACATACAATGGATTCTTGAA+TGG | 0.469129 | 8.4:+31606458 | None:intergenic |
| GAAGAATCATAAACAATATG+AGG | 0.474159 | 8.4:+31625169 | None:intergenic |
| ATTGTTTATGATTCTTCCTG+TGG | 0.493582 | 8.4:-31625163 | MS.gene23849:CDS |
| GAAGTGCAAATATGCCAAAT+GGG | 0.497702 | 8.4:+31609349 | None:intergenic |
| GAATCCATTGTATGTGGTGG+TGG | 0.497824 | 8.4:-31606450 | MS.gene23849:CDS |
| AATGGATTCTTGAATGGTAC+AGG | 0.498388 | 8.4:+31606464 | None:intergenic |
| TCCTCCAAGTGAAGTGTGAT+GGG | 0.501289 | 8.4:+31624909 | None:intergenic |
| CAAAGCAGAAAACCGGAACA+AGG | 0.515171 | 8.4:+31608206 | None:intergenic |
| TTTCCCCATCACACTTCACT+TGG | 0.516494 | 8.4:-31624913 | MS.gene23849:CDS |
| AATAAACTGTTCTTATCCAC+AGG | 0.525451 | 8.4:+31625147 | None:intergenic |
| GTATATCTTAAAGAAAGAAA+AGG | 0.528178 | 8.4:-31608262 | MS.gene23849:CDS |
| TGTTCGAATAGCAATGGAGA+TGG | 0.535110 | 8.4:-31608236 | MS.gene23849:CDS |
| AGGATTTGTTCGAATAGCAA+TGG | 0.537757 | 8.4:-31608242 | MS.gene23849:CDS |
| TCCAGAACCAACATCAGAGC+AGG | 0.538241 | 8.4:-31606402 | None:intergenic |
| TTACCCACCACCACATACAA+TGG | 0.555274 | 8.4:+31606446 | None:intergenic |
| GTTCGAATAGCAATGGAGAT+GGG | 0.558696 | 8.4:-31608235 | MS.gene23849:CDS |
| ATTCAAGAATCCATTGTATG+TGG | 0.565867 | 8.4:-31606456 | MS.gene23849:CDS |
| ATCTTTCCATTAATATTCTG+GGG | 0.566749 | 8.4:-31607602 | MS.gene23849:CDS |
| AAGCAGAAAACCGGAACAAG+GGG | 0.574699 | 8.4:+31608208 | None:intergenic |
| CGTAGACAGATATCTATAAG+TGG | 0.577657 | 8.4:-31607673 | MS.gene23849:intron |
| GTACATACCTGCTCTGATGT+TGG | 0.598523 | 8.4:+31606395 | None:intergenic |
| AAAGCAGAAAACCGGAACAA+GGG | 0.608373 | 8.4:+31608207 | None:intergenic |
| ATCCTCCAAGTGAAGTGTGA+TGG | 0.611933 | 8.4:+31624908 | None:intergenic |
| AGTGTTTCTCTCATTCCACC+AGG | 0.615356 | 8.4:+31608478 | None:intergenic |
| ATTTAAGACAAATATGGACA+TGG | 0.624707 | 8.4:-31608573 | MS.gene23849:CDS |
| AGACAGATATCTATAAGTGG+TGG | 0.640907 | 8.4:-31607670 | MS.gene23849:CDS |
| CCAAATGGGAAAATTGAATG+TGG | 0.677772 | 8.4:+31609363 | None:intergenic |
| CAAGAATCCATTGTATGTGG+TGG | 0.680708 | 8.4:-31606453 | MS.gene23849:CDS |
| AGTTGCATTATAGTACCTGG+TGG | 0.685008 | 8.4:-31608493 | MS.gene23849:CDS |
| AATCCATTGTATGTGGTGGT+GGG | 0.689098 | 8.4:-31606449 | MS.gene23849:CDS |
| GATAGTTGCATTATAGTACC+TGG | 0.700909 | 8.4:-31608496 | MS.gene23849:CDS |
| CCTCCAAGTGAAGTGTGATG+GGG | 0.703428 | 8.4:+31624910 | None:intergenic |
| AGCAGAAAACCGGAACAAGG+GGG | 0.711833 | 8.4:+31608209 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 31606403 | 31625203 | 31606403 | ID=MS.gene23849 |
| chr8.4 | mRNA | 31606403 | 31625203 | 31606403 | ID=MS.gene23849.t1;Parent=MS.gene23849 |
| chr8.4 | exon | 31625129 | 31625203 | 31625129 | ID=MS.gene23849.t1.exon1;Parent=MS.gene23849.t1 |
| chr8.4 | CDS | 31625129 | 31625203 | 31625129 | ID=cds.MS.gene23849.t1;Parent=MS.gene23849.t1 |
| chr8.4 | exon | 31624877 | 31624962 | 31624877 | ID=MS.gene23849.t1.exon2;Parent=MS.gene23849.t1 |
| chr8.4 | CDS | 31624877 | 31624962 | 31624877 | ID=cds.MS.gene23849.t1;Parent=MS.gene23849.t1 |
| chr8.4 | exon | 31609293 | 31609399 | 31609293 | ID=MS.gene23849.t1.exon3;Parent=MS.gene23849.t1 |
| chr8.4 | CDS | 31609293 | 31609399 | 31609293 | ID=cds.MS.gene23849.t1;Parent=MS.gene23849.t1 |
| chr8.4 | exon | 31608456 | 31608611 | 31608456 | ID=MS.gene23849.t1.exon4;Parent=MS.gene23849.t1 |
| chr8.4 | CDS | 31608456 | 31608611 | 31608456 | ID=cds.MS.gene23849.t1;Parent=MS.gene23849.t1 |
| chr8.4 | exon | 31608201 | 31608287 | 31608201 | ID=MS.gene23849.t1.exon5;Parent=MS.gene23849.t1 |
| chr8.4 | CDS | 31608201 | 31608287 | 31608201 | ID=cds.MS.gene23849.t1;Parent=MS.gene23849.t1 |
| chr8.4 | exon | 31607593 | 31607690 | 31607593 | ID=MS.gene23849.t1.exon6;Parent=MS.gene23849.t1 |
| chr8.4 | CDS | 31607593 | 31607690 | 31607593 | ID=cds.MS.gene23849.t1;Parent=MS.gene23849.t1 |
| chr8.4 | exon | 31606403 | 31606490 | 31606403 | ID=MS.gene23849.t1.exon7;Parent=MS.gene23849.t1 |
| chr8.4 | CDS | 31606403 | 31606490 | 31606403 | ID=cds.MS.gene23849.t1;Parent=MS.gene23849.t1 |
| Gene Sequence |
| Protein sequence |
