Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24836.t1 | XP_013458428.1 | 95.9 | 121 | 1 | 1 | 1 | 121 | 1 | 117 | 4.40E-47 | 197.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24836.t1 | B7FH97 | 95.9 | 121 | 1 | 1 | 1 | 121 | 1 | 117 | 3.1e-47 | 197.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049173 | MS.gene24836 | 0.807077 | 6.03E-50 | -1.69E-46 |
| MS.gene049615 | MS.gene24836 | 0.805009 | 1.64E-49 | -1.69E-46 |
| MS.gene050767 | MS.gene24836 | 0.82862 | 8.12E-55 | -1.69E-46 |
| MS.gene050770 | MS.gene24836 | 0.828082 | 1.10E-54 | -1.69E-46 |
| MS.gene051692 | MS.gene24836 | 0.843707 | 1.19E-58 | -1.69E-46 |
| MS.gene052322 | MS.gene24836 | 0.823561 | 1.30E-53 | -1.69E-46 |
| MS.gene053103 | MS.gene24836 | 0.809716 | 1.65E-50 | -1.69E-46 |
| MS.gene053209 | MS.gene24836 | 0.802026 | 6.82E-49 | -1.69E-46 |
| MS.gene054376 | MS.gene24836 | 0.821326 | 4.29E-53 | -1.69E-46 |
| MS.gene054600 | MS.gene24836 | 0.802291 | 6.01E-49 | -1.69E-46 |
| MS.gene055254 | MS.gene24836 | 0.818095 | 2.34E-52 | -1.69E-46 |
| MS.gene055362 | MS.gene24836 | 0.804985 | 1.66E-49 | -1.69E-46 |
| MS.gene055499 | MS.gene24836 | 0.825096 | 5.65E-54 | -1.69E-46 |
| MS.gene056374 | MS.gene24836 | 0.820894 | 5.39E-53 | -1.69E-46 |
| MS.gene057129 | MS.gene24836 | 0.841424 | 4.79E-58 | -1.69E-46 |
| MS.gene057295 | MS.gene24836 | 0.870327 | 1.59E-66 | -1.69E-46 |
| MS.gene057416 | MS.gene24836 | 0.811227 | 7.78E-51 | -1.69E-46 |
| MS.gene058019 | MS.gene24836 | 0.836522 | 8.91E-57 | -1.69E-46 |
| MS.gene058293 | MS.gene24836 | 0.834307 | 3.23E-56 | -1.69E-46 |
| MS.gene058787 | MS.gene24836 | 0.808952 | 2.40E-50 | -1.69E-46 |
| MS.gene059692 | MS.gene24836 | 0.80596 | 1.04E-49 | -1.69E-46 |
| MS.gene059695 | MS.gene24836 | 0.811317 | 7.44E-51 | -1.69E-46 |
| MS.gene061200 | MS.gene24836 | 0.814528 | 1.47E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24836.t1 | MTR_4g125600 | 95.868 | 121 | 1 | 1 | 1 | 121 | 1 | 117 | 4.33e-74 | 216 |
| MS.gene24836.t1 | MTR_3g104950 | 57.724 | 123 | 45 | 5 | 1 | 120 | 1 | 119 | 1.51e-35 | 118 |
| MS.gene24836.t1 | MTR_1g015290 | 58.730 | 126 | 44 | 5 | 1 | 120 | 1 | 124 | 3.46e-34 | 115 |
| MS.gene24836.t1 | MTR_2g041260 | 65.289 | 121 | 39 | 3 | 1 | 120 | 1 | 119 | 7.10e-33 | 112 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24836.t1 | AT2G06520 | 57.724 | 123 | 41 | 4 | 1 | 120 | 1 | 115 | 3.85e-29 | 102 |
Find 27 sgRNAs with CRISPR-Local
Find 26 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCTTGCTCAGCATCGTTTC+TGG | 0.146677 | 8.4:+9339878 | MS.gene24836:CDS |
| GCTATTCTTGGTGCTGTTAT+TGG | 0.188543 | 8.4:+9339917 | MS.gene24836:CDS |
| TTCTTGCTGGCACAAGTTAA+TGG | 0.260311 | 8.4:-9339641 | None:intergenic |
| TCGCTTAACAGGATCGAAAT+TGG | 0.294106 | 8.4:-9339945 | None:intergenic |
| GTTGTGGTTACTGCTATTCT+TGG | 0.319417 | 8.4:+9339905 | MS.gene24836:CDS |
| ATTCATCAACCTCGCTTAAC+AGG | 0.324240 | 8.4:-9339956 | None:intergenic |
| TGGTGCCTCTAGCTTCTTGC+TGG | 0.342131 | 8.4:-9339654 | None:intergenic |
| TGTCACAAGTTTCTCTTTCA+AGG | 0.346815 | 8.4:-9339771 | None:intergenic |
| GTTGCTGCCACAGACTTTGA+TGG | 0.351954 | 8.4:-9339716 | None:intergenic |
| TTCAACTTCCACAATTTCAA+TGG | 0.410680 | 8.4:+9339613 | MS.gene24836:CDS |
| AACCTCTCAAGCATTCTTGA+AGG | 0.466332 | 8.4:+9339676 | MS.gene24836:CDS |
| TTTGATGGCTTCAAAGTCAA+TGG | 0.476094 | 8.4:-9339701 | None:intergenic |
| TTGAAAGAGAAACTTGTGAC+AGG | 0.488854 | 8.4:+9339773 | MS.gene24836:CDS |
| GTAGCAGCTTCAGCCACATC+AGG | 0.488989 | 8.4:-9339833 | None:intergenic |
| TTCAATGATTTCTCCTGATG+TGG | 0.495622 | 8.4:+9339820 | MS.gene24836:CDS |
| TTCAAGAATGCTTGAGAGGT+TGG | 0.501532 | 8.4:-9339674 | None:intergenic |
| TGGCACAGCCATTGAAATTG+TGG | 0.527378 | 8.4:-9339621 | None:intergenic |
| CATCGTTTCTGGTGGTGTTG+TGG | 0.559998 | 8.4:+9339889 | MS.gene24836:CDS |
| AGCAAGAAATTCTTGAGAGA+AGG | 0.566334 | 8.4:-9339863 | None:intergenic |
| TTGCTCAGCATCGTTTCTGG+TGG | 0.567414 | 8.4:+9339881 | MS.gene24836:CDS |
| CAGCAACAAACCGCAACGCA+AGG | 0.602807 | 8.4:+9339732 | MS.gene24836:CDS |
| TCACTACAAACCTTGCGTTG+CGG | 0.628582 | 8.4:-9339742 | None:intergenic |
| AATTTCGATCCTGTTAAGCG+AGG | 0.632764 | 8.4:+9339947 | MS.gene24836:CDS |
| TGAAAGAGAAACTTGTGACA+GGG | 0.650173 | 8.4:+9339774 | MS.gene24836:CDS |
| TTTGAAGCCATCAAAGTCTG+TGG | 0.657555 | 8.4:+9339709 | MS.gene24836:CDS |
| TGCCTTCAAGAATGCTTGAG+AGG | 0.659518 | 8.4:-9339678 | None:intergenic |
| TTGTGCCAGCAAGAAGCTAG+AGG | 0.660281 | 8.4:+9339649 | MS.gene24836:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TTCAACTTCCACAATTTCAA+TGG | + | chr8.4:9339613-9339632 | MS.gene24836:CDS | 30.0% | |
| AGCAAGAAATTCTTGAGAGA+AGG | - | chr8.4:9339866-9339885 | None:intergenic | 35.0% | |
| TGAAAGAGAAACTTGTGACA+GGG | + | chr8.4:9339774-9339793 | MS.gene24836:CDS | 35.0% | |
| TGTCACAAGTTTCTCTTTCA+AGG | - | chr8.4:9339774-9339793 | None:intergenic | 35.0% | |
| TTCAATGATTTCTCCTGATG+TGG | + | chr8.4:9339820-9339839 | MS.gene24836:CDS | 35.0% | |
| TTGAAAGAGAAACTTGTGAC+AGG | + | chr8.4:9339773-9339792 | MS.gene24836:CDS | 35.0% | |
| ! | TTTGATGGCTTCAAAGTCAA+TGG | - | chr8.4:9339704-9339723 | None:intergenic | 35.0% |
| AACCTCTCAAGCATTCTTGA+AGG | + | chr8.4:9339676-9339695 | MS.gene24836:CDS | 40.0% | |
| AATTTCGATCCTGTTAAGCG+AGG | + | chr8.4:9339947-9339966 | MS.gene24836:CDS | 40.0% | |
| GTTGTGGTTACTGCTATTCT+TGG | + | chr8.4:9339905-9339924 | MS.gene24836:CDS | 40.0% | |
| TCGCTTAACAGGATCGAAAT+TGG | - | chr8.4:9339948-9339967 | None:intergenic | 40.0% | |
| TTCAAGAATGCTTGAGAGGT+TGG | - | chr8.4:9339677-9339696 | None:intergenic | 40.0% | |
| TTCTTGCTGGCACAAGTTAA+TGG | - | chr8.4:9339644-9339663 | None:intergenic | 40.0% | |
| ! | TTTGAAGCCATCAAAGTCTG+TGG | + | chr8.4:9339709-9339728 | MS.gene24836:CDS | 40.0% |
| !! | GCTATTCTTGGTGCTGTTAT+TGG | + | chr8.4:9339917-9339936 | MS.gene24836:CDS | 40.0% |
| TCACTACAAACCTTGCGTTG+CGG | - | chr8.4:9339745-9339764 | None:intergenic | 45.0% | |
| TGCCTTCAAGAATGCTTGAG+AGG | - | chr8.4:9339681-9339700 | None:intergenic | 45.0% | |
| TGGCACAGCCATTGAAATTG+TGG | - | chr8.4:9339624-9339643 | None:intergenic | 45.0% | |
| TTCTTGCTCAGCATCGTTTC+TGG | + | chr8.4:9339878-9339897 | MS.gene24836:CDS | 45.0% | |
| GTTGCTGCCACAGACTTTGA+TGG | - | chr8.4:9339719-9339738 | None:intergenic | 50.0% | |
| TTGTGCCAGCAAGAAGCTAG+AGG | + | chr8.4:9339649-9339668 | MS.gene24836:CDS | 50.0% | |
| ! | TTGCTCAGCATCGTTTCTGG+TGG | + | chr8.4:9339881-9339900 | MS.gene24836:CDS | 50.0% |
| !! | CATCGTTTCTGGTGGTGTTG+TGG | + | chr8.4:9339889-9339908 | MS.gene24836:CDS | 50.0% |
| CAGCAACAAACCGCAACGCA+AGG | + | chr8.4:9339732-9339751 | MS.gene24836:CDS | 55.0% | |
| GTAGCAGCTTCAGCCACATC+AGG | - | chr8.4:9339836-9339855 | None:intergenic | 55.0% | |
| ! | TGGTGCCTCTAGCTTCTTGC+TGG | - | chr8.4:9339657-9339676 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 9339608 | 9339973 | 9339608 | ID=MS.gene24836 |
| chr8.4 | mRNA | 9339608 | 9339973 | 9339608 | ID=MS.gene24836.t1;Parent=MS.gene24836 |
| chr8.4 | exon | 9339608 | 9339973 | 9339608 | ID=MS.gene24836.t1.exon1;Parent=MS.gene24836.t1 |
| chr8.4 | CDS | 9339608 | 9339973 | 9339608 | ID=cds.MS.gene24836.t1;Parent=MS.gene24836.t1 |
| Gene Sequence |
| Protein sequence |
