AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene25274


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene25274.t1 MTR_8g062700 95.122 123 6 0 1 123 558 680 2.40e-76 249
MS.gene25274.t1 MTR_8g062700 95.122 123 6 0 1 123 559 681 2.41e-76 249
MS.gene25274.t1 MTR_5g036670 74.797 123 31 0 1 123 555 677 1.71e-60 204
MS.gene25274.t1 MTR_6g008970 64.228 123 44 0 1 123 541 663 3.38e-52 180
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene25274.t1 AT1G24190 74.797 123 31 0 1 123 497 619 3.29e-61 206
MS.gene25274.t1 AT1G24190 74.797 123 31 0 1 123 493 615 3.50e-61 206
MS.gene25274.t1 AT1G24190 74.797 123 31 0 1 123 493 615 3.77e-61 206
MS.gene25274.t1 AT1G70060 73.171 123 33 0 1 123 511 633 3.52e-59 200
MS.gene25274.t1 AT1G70060 73.171 123 33 0 1 123 511 633 3.52e-59 200
MS.gene25274.t1 AT1G59890 73.984 123 32 0 1 123 386 508 2.19e-58 197
MS.gene25274.t1 AT1G59890 73.984 123 32 0 1 123 382 504 2.34e-58 197
MS.gene25274.t1 AT1G59890 73.984 123 32 0 1 123 391 513 2.47e-58 197
MS.gene25274.t1 AT1G59890 73.984 123 32 0 1 123 386 508 2.54e-58 197
MS.gene25274.t1 AT1G10450 70.732 123 36 0 1 123 369 491 2.32e-54 186
MS.gene25274.t1 AT1G10450 70.732 123 36 0 1 123 369 491 2.32e-54 186
MS.gene25274.t1 AT1G10450 70.732 123 36 0 1 123 420 542 2.69e-54 186
MS.gene25274.t1 AT5G15020 65.041 123 43 0 1 123 533 655 4.25e-52 179
MS.gene25274.t1 AT5G15020 65.041 123 43 0 1 123 533 655 4.50e-52 179
MS.gene25274.t1 AT3G01320 61.789 123 47 0 1 123 538 660 1.31e-48 169
MS.gene25274.t1 AT3G01320 61.789 123 47 0 1 123 526 648 1.39e-48 169

Find 49 sgRNAs with CRISPR-Local

Find 82 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGTATTGAGGAGCATTTATC+AGG 0.277753 8.3:+39902986 MS.gene25274:CDS
GTGCAGAGTTTAATATAGTT+TGG 0.279806 8.3:+39903267 MS.gene25274:CDS
GAATGCATCTCTAGCCTTGC+CGG 0.346779 8.3:+39903196 MS.gene25274:CDS
TGCAGAGTTTAATATAGTTT+GGG 0.348281 8.3:+39903268 MS.gene25274:CDS
ACTCCTCCTGTTTCTGCTTC+AGG 0.353246 8.3:-39903232 None:intergenic
GTTAATGATGATGTAATTCC+AGG 0.355314 8.3:+39902950 MS.gene25274:CDS
GGATGGTGCTGTTACTGTTC+AGG 0.386199 8.3:+39903925 MS.gene25274:CDS
AAATGCTCCTCAATACGAAT+TGG 0.386991 8.3:-39902980 None:intergenic
GAGCAGGCTGTCGACGGGAC+TGG 0.402053 8.3:+39903963 MS.gene25274:CDS
AACCCGCCTGAAGCAGAAAC+AGG 0.442327 8.3:+39903226 MS.gene25274:CDS
AGGTGCATTGAACGACTATA+CGG 0.453204 8.3:+39903137 MS.gene25274:CDS
ATACGAATTGGAATATCTCC+TGG 0.466439 8.3:-39902968 None:intergenic
AGATATTCCAATTCGTATTG+AGG 0.495294 8.3:+39902973 MS.gene25274:CDS
GAGAAAAGACATTATTTCAA+AGG 0.496041 8.3:+39903904 MS.gene25274:CDS
AAAGACATTATTTCAAAGGA+TGG 0.504914 8.3:+39903908 MS.gene25274:CDS
CGGGACTGGAGCGGAGATTG+TGG 0.507792 8.3:+39903977 MS.gene25274:CDS
CTCCTGTTTCTGCTTCAGGC+GGG 0.509322 8.3:-39903228 None:intergenic
AGTTACACCGGCGGGATCAC+TGG 0.518021 8.3:-39903867 None:intergenic
CAAATACTTGGCTGAAGTGA+AGG 0.518215 8.3:+39903817 MS.gene25274:CDS
TTCAGGCGGGTTAATATCAC+CGG 0.529652 8.3:-39903215 None:intergenic
CCTCCTGTTTCTGCTTCAGG+CGG 0.534175 8.3:-39903229 None:intergenic
GAAGGTGAGGAAGGCAGCCG+AGG 0.543484 8.3:+39903835 MS.gene25274:CDS
TTCTGAGAGAAACAAATACT+TGG 0.543933 8.3:+39903805 MS.gene25274:CDS
TGACCATGGGCTTGATGTGA+TGG 0.544947 8.3:+39903160 MS.gene25274:CDS
AGCTTCGAGCAGGCTGTCGA+CGG 0.546334 8.3:+39903957 MS.gene25274:CDS
GCTTCGAGCAGGCTGTCGAC+GGG 0.549946 8.3:+39903958 MS.gene25274:CDS
GTTGATACGGAGCTTCGAGC+AGG 0.550606 8.3:+39903947 MS.gene25274:CDS
GAACGACTATACGGTGACCA+TGG 0.550770 8.3:+39903146 MS.gene25274:CDS
TGTTACTGTTCAGGTTGATA+CGG 0.551073 8.3:+39903934 MS.gene25274:CDS
GGCTGAAGTGAAGGTGAGGA+AGG 0.554813 8.3:+39903826 MS.gene25274:CDS
GAAGTCGTCATGGGCAACAT+TGG 0.556203 8.3:-39903785 None:intergenic
AACGACTATACGGTGACCAT+GGG 0.556588 8.3:+39903147 MS.gene25274:CDS
TTTCTCTCAGAAGTCGTCAT+GGG 0.563812 8.3:-39903794 None:intergenic
GAAGCAGAAACAGGAGGAGT+GGG 0.580158 8.3:+39903235 MS.gene25274:CDS
GTTTCTCTCAGAAGTCGTCA+TGG 0.580421 8.3:-39903795 None:intergenic
AAACTATATTAAACTCTGCA+CGG 0.581815 8.3:-39903265 None:intergenic
TGAAGCAGAAACAGGAGGAG+TGG 0.583162 8.3:+39903234 MS.gene25274:CDS
GGCTGTCGACGGGACTGGAG+CGG 0.601171 8.3:+39903968 MS.gene25274:CDS
AAGGTGAGGAAGGCAGCCGA+GGG 0.608404 8.3:+39903836 MS.gene25274:CDS
TGCAACAAGTAAGAAAGTTG+AGG 0.611189 8.3:+39902913 MS.gene25274:CDS
TTACGATGAGTTACACCGGC+GGG 0.613809 8.3:-39903875 None:intergenic
ATCACTGGTAGCATATCCCT+CGG 0.627935 8.3:-39903852 None:intergenic
TGCTTTACGATGAGTTACAC+CGG 0.645166 8.3:-39903879 None:intergenic
CTTCCATCACATCAAGCCCA+TGG 0.664006 8.3:-39903163 None:intergenic
ACTTGGCTGAAGTGAAGGTG+AGG 0.666702 8.3:+39903822 MS.gene25274:CDS
GCGGGTTAATATCACCGGCA+AGG 0.668769 8.3:-39903210 None:intergenic
TATGCTACCAGTGATCCCGC+CGG 0.678025 8.3:+39903860 MS.gene25274:CDS
CCGCCTGAAGCAGAAACAGG+AGG 0.700575 8.3:+39903229 MS.gene25274:CDS
TTTACGATGAGTTACACCGG+CGG 0.801865 8.3:-39903876 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATTTCTTAACTATATTAAA+AGG - chr8.3:39903507-39903526 None:intergenic 10.0%
!! TTAAATAACTTACTATTTAA+AGG - chr8.3:39903454-39903473 None:intergenic 10.0%
!!! GAAATATTTAAAATTAGTTT+AGG + chr8.3:39903521-39903540 MS.gene25274:intron 10.0%
!! AATTTATCATAGCATATAAA+TGG + chr8.3:39903036-39903055 MS.gene25274:intron 15.0%
!!! AATCAAATATTTTCTTTCAA+TGG - chr8.3:39903480-39903499 None:intergenic 15.0%
!! TATTTAAAGGTTGTTAAAGA+GGG - chr8.3:39903441-39903460 None:intergenic 20.0%
!!! CAATTCTTGATTCTTTTTAA+CGG + chr8.3:39903410-39903429 MS.gene25274:intron 20.0%
! AAACTATATTAAACTCTGCA+CGG - chr8.3:39903268-39903287 None:intergenic 25.0%
! AAAGACATTATTTCAAAGGA+TGG + chr8.3:39903908-39903927 MS.gene25274:CDS 25.0%
! AGAATCAAGAATTGTGTTAT+TGG - chr8.3:39903404-39903423 None:intergenic 25.0%
! ATAAACTCTTGAATAAGGAA+GGG + chr8.3:39903653-39903672 MS.gene25274:intron 25.0%
! ATTCAAGAGTTTATGGATAT+CGG - chr8.3:39903647-39903666 None:intergenic 25.0%
! CTATTTAAAGGTTGTTAAAG+AGG - chr8.3:39903442-39903461 None:intergenic 25.0%
! GAGAAAAGACATTATTTCAA+AGG + chr8.3:39903904-39903923 MS.gene25274:CDS 25.0%
! TATCCATAAACTCTTGAATA+AGG + chr8.3:39903648-39903667 MS.gene25274:intron 25.0%
! TGCAGAGTTTAATATAGTTT+GGG + chr8.3:39903268-39903287 MS.gene25274:CDS 25.0%
! TGTCAACAAATAATGAAAAG+AGG - chr8.3:39903746-39903765 None:intergenic 25.0%
!!! TTGTTTTAGCTCTAAATCTT+AGG + chr8.3:39903117-39903136 MS.gene25274:intron 25.0%
AGAACAGAACACAAAATATC+AGG - chr8.3:39903077-39903096 None:intergenic 30.0%
AGATATTCCAATTCGTATTG+AGG + chr8.3:39902973-39902992 MS.gene25274:CDS 30.0%
CATAAACTCTTGAATAAGGA+AGG + chr8.3:39903652-39903671 MS.gene25274:intron 30.0%
CTTCCTTATTCAAGAGTTTA+TGG - chr8.3:39903654-39903673 None:intergenic 30.0%
GTGCAGAGTTTAATATAGTT+TGG + chr8.3:39903267-39903286 MS.gene25274:CDS 30.0%
TAAACTCTTGAATAAGGAAG+GGG + chr8.3:39903654-39903673 MS.gene25274:intron 30.0%
! CTTGATTCTTTTTAACGGTA+TGG + chr8.3:39903415-39903434 MS.gene25274:intron 30.0%
! GTTAATGATGATGTAATTCC+AGG + chr8.3:39902950-39902969 MS.gene25274:CDS 30.0%
! TTCTGAGAGAAACAAATACT+TGG + chr8.3:39903805-39903824 MS.gene25274:CDS 30.0%
AAATGCTCCTCAATACGAAT+TGG - chr8.3:39902983-39903002 None:intergenic 35.0%
ACAAAAAGCTTGAGCACTAA+AGG + chr8.3:39903359-39903378 MS.gene25274:CDS 35.0%
ATACGAATTGGAATATCTCC+TGG - chr8.3:39902971-39902990 None:intergenic 35.0%
CTGTTCTGATACTATCTTCT+TGG + chr8.3:39903090-39903109 MS.gene25274:intron 35.0%
TGCAACAAGTAAGAAAGTTG+AGG + chr8.3:39902913-39902932 MS.gene25274:CDS 35.0%
! AAGCTTTTACTTTAAGCAGC+AGG + chr8.3:39903334-39903353 MS.gene25274:CDS 35.0%
!! TGTTACTGTTCAGGTTGATA+CGG + chr8.3:39903934-39903953 MS.gene25274:CDS 35.0%
AGCCCAAACCAATATTAGCA+GGG + chr8.3:39903589-39903608 MS.gene25274:intron 40.0%
AGGTGCATTGAACGACTATA+CGG + chr8.3:39903137-39903156 MS.gene25274:CDS 40.0%
CAAATACTTGGCTGAAGTGA+AGG + chr8.3:39903817-39903836 MS.gene25274:CDS 40.0%
CTTGAATAAGGAAGGGGATA+CGG + chr8.3:39903660-39903679 MS.gene25274:intron 40.0%
TGCTTTACGATGAGTTACAC+CGG - chr8.3:39903882-39903901 None:intergenic 40.0%
TTTCTCTCAGAAGTCGTCAT+GGG - chr8.3:39903797-39903816 None:intergenic 40.0%
!! CGTATTGAGGAGCATTTATC+AGG + chr8.3:39902986-39903005 MS.gene25274:CDS 40.0%
AAACCAATATTAGCAGGGCG+TGG + chr8.3:39903594-39903613 MS.gene25274:intron 45.0%
AACGACTATACGGTGACCAT+GGG + chr8.3:39903147-39903166 MS.gene25274:CDS 45.0%
ATCACTGGTAGCATATCCCT+CGG - chr8.3:39903855-39903874 None:intergenic 45.0%
CAGCCCAAACCAATATTAGC+AGG + chr8.3:39903588-39903607 MS.gene25274:intron 45.0%
GTTTCTCTCAGAAGTCGTCA+TGG - chr8.3:39903798-39903817 None:intergenic 45.0%
TTTACGATGAGTTACACCGG+CGG - chr8.3:39903879-39903898 None:intergenic 45.0%
! ACGCCCTGCTAATATTGGTT+TGG - chr8.3:39903595-39903614 None:intergenic 45.0%
!! CGCCCTGCTAATATTGGTTT+GGG - chr8.3:39903594-39903613 None:intergenic 45.0%
!! TTCAGGCGGGTTAATATCAC+CGG - chr8.3:39903218-39903237 None:intergenic 45.0%
!!! AAATATTTAAAATTAGTTTA+GGG + chr8.3:39903522-39903541 MS.gene25274:intron 5.0%
ACTTGGCTGAAGTGAAGGTG+AGG + chr8.3:39903822-39903841 MS.gene25274:CDS 50.0%
CAATATTAGCAGGGCGTGGT+GGG + chr8.3:39903598-39903617 MS.gene25274:intron 50.0%
CTTCCATCACATCAAGCCCA+TGG - chr8.3:39903166-39903185 None:intergenic 50.0%
GAACGACTATACGGTGACCA+TGG + chr8.3:39903146-39903165 MS.gene25274:CDS 50.0%
GAAGCAGAAACAGGAGGAGT+GGG + chr8.3:39903235-39903254 MS.gene25274:CDS 50.0%
GAAGTCGTCATGGGCAACAT+TGG - chr8.3:39903788-39903807 None:intergenic 50.0%
GAATAAGGAAGGGGATACGG+AGG + chr8.3:39903663-39903682 MS.gene25274:intron 50.0%
TGAAGCAGAAACAGGAGGAG+TGG + chr8.3:39903234-39903253 MS.gene25274:CDS 50.0%
TGACCATGGGCTTGATGTGA+TGG + chr8.3:39903160-39903179 MS.gene25274:CDS 50.0%
TTACGATGAGTTACACCGGC+GGG - chr8.3:39903878-39903897 None:intergenic 50.0%
!! ACTCCTCCTGTTTCTGCTTC+AGG - chr8.3:39903235-39903254 None:intergenic 50.0%
!! GAATGCATCTCTAGCCTTGC+CGG + chr8.3:39903196-39903215 MS.gene25274:CDS 50.0%
!! GGATGGTGCTGTTACTGTTC+AGG + chr8.3:39903925-39903944 MS.gene25274:CDS 50.0%
AACCCGCCTGAAGCAGAAAC+AGG + chr8.3:39903226-39903245 MS.gene25274:CDS 55.0%
CCAATATTAGCAGGGCGTGG+TGG + chr8.3:39903597-39903616 MS.gene25274:intron 55.0%
CCACCACGCCCTGCTAATAT+TGG - chr8.3:39903600-39903619 None:intergenic 55.0%
GGCTGAAGTGAAGGTGAGGA+AGG + chr8.3:39903826-39903845 MS.gene25274:CDS 55.0%
GTTGATACGGAGCTTCGAGC+AGG + chr8.3:39903947-39903966 MS.gene25274:CDS 55.0%
TATGCTACCAGTGATCCCGC+CGG + chr8.3:39903860-39903879 MS.gene25274:CDS 55.0%
! GCGGGTTAATATCACCGGCA+AGG - chr8.3:39903213-39903232 None:intergenic 55.0%
!! CCTCCTGTTTCTGCTTCAGG+CGG - chr8.3:39903232-39903251 None:intergenic 55.0%
!! CTCCTGTTTCTGCTTCAGGC+GGG - chr8.3:39903231-39903250 None:intergenic 55.0%
AAGGTGAGGAAGGCAGCCGA+GGG + chr8.3:39903836-39903855 MS.gene25274:CDS 60.0%
AGCTTCGAGCAGGCTGTCGA+CGG + chr8.3:39903957-39903976 MS.gene25274:CDS 60.0%
AGTTACACCGGCGGGATCAC+TGG - chr8.3:39903870-39903889 None:intergenic 60.0%
CCGCCTGAAGCAGAAACAGG+AGG + chr8.3:39903229-39903248 MS.gene25274:CDS 60.0%
CGGGACTGGAGCGGAGATTG+TGG + chr8.3:39903977-39903996 MS.gene25274:CDS 65.0%
GAAGGTGAGGAAGGCAGCCG+AGG + chr8.3:39903835-39903854 MS.gene25274:CDS 65.0%
GCTTCGAGCAGGCTGTCGAC+GGG + chr8.3:39903958-39903977 MS.gene25274:CDS 65.0%
GAGCAGGCTGTCGACGGGAC+TGG + chr8.3:39903963-39903982 MS.gene25274:CDS 70.0%
GGCTGTCGACGGGACTGGAG+CGG + chr8.3:39903968-39903987 MS.gene25274:CDS 70.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 39902893 39904009 39902893 ID=MS.gene25274
chr8.3 mRNA 39902893 39904009 39902893 ID=MS.gene25274.t1;Parent=MS.gene25274
chr8.3 exon 39902893 39903007 39902893 ID=MS.gene25274.t1.exon1;Parent=MS.gene25274.t1
chr8.3 CDS 39902893 39903007 39902893 ID=cds.MS.gene25274.t1;Parent=MS.gene25274.t1
chr8.3 exon 39903126 39903380 39903126 ID=MS.gene25274.t1.exon2;Parent=MS.gene25274.t1
chr8.3 CDS 39903126 39903380 39903126 ID=cds.MS.gene25274.t1;Parent=MS.gene25274.t1
chr8.3 exon 39903783 39904009 39903783 ID=MS.gene25274.t1.exon3;Parent=MS.gene25274.t1
chr8.3 CDS 39903783 39904009 39903783 ID=cds.MS.gene25274.t1;Parent=MS.gene25274.t1
Gene Sequence

>MS.gene25274

ATGTTGTTAGAGTCTATAAATGCAACAAGTAAGAAAGTTGAGGAGATCATAGAAAAAGTTAATGATGATGTAATTCCAGGAGATATTCCAATTCGTATTGAGGAGCATTTATCAGGTTATATGTTTTTTCAATTGATCAATGAAATTTATCATAGCATATAAATGGAAAATGATGTAATTTCCTGATATTTTGTGTTCTGTTCTGATACTATCTTCTTGGTGTGTTGTTTTAGCTCTAAATCTTAGGTGCATTGAACGACTATACGGTGACCATGGGCTTGATGTGATGGAAGTGTTGAAAAAGAATGCATCTCTAGCCTTGCCGGTGATATTAACCCGCCTGAAGCAGAAACAGGAGGAGTGGGCAAGATGCCGTGCAGAGTTTAATATAGTTTGGGCTGATGTATATGCGAAGAATTATCATAAATCACTTGATCATCGAAGCTTTTACTTTAAGCAGCAGGATACAAAAAGCTTGAGCACTAAAGGTAAAATATTAATTTTCTGTCCAATAACACAATTCTTGATTCTTTTTAACGGTATGGCCCTCTTTAACAACCTTTAAATAGTAAGTTATTTAACATCCATTGAAAGAAAATATTTGATTTAATCCTTTTAATATAGTTAAGAAATATTTAAAATTAGTTTAGGGAATAAAGTATACTTAATTAATTTAATGTAGTTAGTTAAGAACACAGCCCAAACCAATATTAGCAGGGCGTGGTGGGCATTTTAGACAAGATGTTGCTCTCCGATATCCATAAACTCTTGAATAAGGAAGGGGATACGGAGGATCTAAATGAATATTTGCGTGAGTGTTTTTGTCTGAGAATTGCTTTCTTCATGCTTGCCTCTTTTCATTATTTGTTGACATTGTTTTTATTTTGTAGAACCAATGTTGCCCATGACGACTTCTGAGAGAAACAAATACTTGGCTGAAGTGAAGGTGAGGAAGGCAGCCGAGGGATATGCTACCAGTGATCCCGCCGGTGTAACTCATCGTAAAGCAAAGAGAAAAGACATTATTTCAAAGGATGGTGCTGTTACTGTTCAGGTTGATACGGAGCTTCGAGCAGGCTGTCGACGGGACTGGAGCGGAGATTGTGGATTCTCCTAA

Protein sequence

>MS.gene25274.t1

MLLESINATSKKVEEIIEKVNDDVIPGDIPIRIEEHLSALNLRCIERLYGDHGLDVMEVLKKNASLALPVILTRLKQKQEEWARCRAEFNIVWADVYAKNYHKSLDHRSFYFKQQDTKSLSTKEPMLPMTTSERNKYLAEVKVRKAAEGYATSDPAGVTHRKAKRKDIISKDGAVTVQVDTELRAGCRRDWSGDCGFS