AlfalfaGEDB | MS.gene25274

M. sativa cultivar XinJiangDaYe / MS.gene25274

KEGG: K11644
GO: -
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene25274.t1	XP_003628621.2	95.1	123	6	0	1	123	559	681	2.10E-59	238.8

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene25274.t1	O48686	74.8	123	31	0	1	123	497	619	1.0e-49	198.0

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene25274.t1	G7LEQ2	95.1	123	6	0	1	123	559	681	1.5e-59	238.8

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049159	MS.gene25274	0.802653	5.07E-49	-1.69E-46
MS.gene049382	MS.gene25274	0.810485	1.13E-50	-1.69E-46
MS.gene050763	MS.gene25274	0.800103	1.69E-48	-1.69E-46
MS.gene050807	MS.gene25274	0.830414	2.98E-55	-1.69E-46
MS.gene051102	MS.gene25274	0.813856	2.07E-51	-1.69E-46
MS.gene051180	MS.gene25274	0.802856	4.60E-49	-1.69E-46
MS.gene052323	MS.gene25274	0.827857	1.24E-54	-1.69E-46
MS.gene052665	MS.gene25274	0.828038	1.12E-54	-1.69E-46
MS.gene053565	MS.gene25274	0.816164	6.37E-52	-1.69E-46
MS.gene055469	MS.gene25274	0.806839	6.77E-50	-1.69E-46

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene25274.t1	MTR_8g062700	95.122	123	6	1	123	558	680	2.40e-76	249
MS.gene25274.t1	MTR_8g062700	95.122	123	6	1	123	559	681	2.41e-76	249
MS.gene25274.t1	MTR_5g036670	74.797	123	31	1	123	555	677	1.71e-60	204
MS.gene25274.t1	MTR_6g008970	64.228	123	44	1	123	541	663	3.38e-52	180

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Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene25274.t1	AT1G24190	74.797	123	31	1	123	497	619	3.29e-61	206
MS.gene25274.t1	AT1G24190	74.797	123	31	1	123	493	615	3.50e-61	206
MS.gene25274.t1	AT1G24190	74.797	123	31	1	123	493	615	3.77e-61	206
MS.gene25274.t1	AT1G70060	73.171	123	33	1	123	511	633	3.52e-59	200
MS.gene25274.t1	AT1G70060	73.171	123	33	1	123	511	633	3.52e-59	200
MS.gene25274.t1	AT1G59890	73.984	123	32	1	123	386	508	2.19e-58	197
MS.gene25274.t1	AT1G59890	73.984	123	32	1	123	382	504	2.34e-58	197
MS.gene25274.t1	AT1G59890	73.984	123	32	1	123	391	513	2.47e-58	197
MS.gene25274.t1	AT1G59890	73.984	123	32	1	123	386	508	2.54e-58	197
MS.gene25274.t1	AT1G10450	70.732	123	36	1	123	369	491	2.32e-54	186

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Find 49 sgRNAs with CRISPR-Local

Find 82 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACTATATTAAACTCTGCA+CGG	0.581815	8.3:-39903265	None:intergenic
AAAGACATTATTTCAAAGGA+TGG	0.504914	8.3:+39903908	MS.gene25274:CDS
AAATGCTCCTCAATACGAAT+TGG	0.386991	8.3:-39902980	None:intergenic
AACCCGCCTGAAGCAGAAAC+AGG	0.442327	8.3:+39903226	MS.gene25274:CDS
AACGACTATACGGTGACCAT+GGG	0.556588	8.3:+39903147	MS.gene25274:CDS
AAGGTGAGGAAGGCAGCCGA+GGG	0.608404	8.3:+39903836	MS.gene25274:CDS
ACTCCTCCTGTTTCTGCTTC+AGG	0.353246	8.3:-39903232	None:intergenic
ACTTGGCTGAAGTGAAGGTG+AGG	0.666702	8.3:+39903822	MS.gene25274:CDS
AGATATTCCAATTCGTATTG+AGG	0.495294	8.3:+39902973	MS.gene25274:CDS
AGCTTCGAGCAGGCTGTCGA+CGG	0.546334	8.3:+39903957	MS.gene25274:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AGAACAGAACACAAAATATC+AGG	-	chr8.3:39903077-39903096	None:intergenic	30.0%
AGATATTCCAATTCGTATTG+AGG	+	chr8.3:39902973-39902992	MS.gene25274:CDS	30.0%
CATAAACTCTTGAATAAGGA+AGG	+	chr8.3:39903652-39903671	MS.gene25274:intron	30.0%
CTTCCTTATTCAAGAGTTTA+TGG	-	chr8.3:39903654-39903673	None:intergenic	30.0%
GTGCAGAGTTTAATATAGTT+TGG	+	chr8.3:39903267-39903286	MS.gene25274:CDS	30.0%
TAAACTCTTGAATAAGGAAG+GGG	+	chr8.3:39903654-39903673	MS.gene25274:intron	30.0%
AAATGCTCCTCAATACGAAT+TGG	-	chr8.3:39902983-39903002	None:intergenic	35.0%
ACAAAAAGCTTGAGCACTAA+AGG	+	chr8.3:39903359-39903378	MS.gene25274:CDS	35.0%
ATACGAATTGGAATATCTCC+TGG	-	chr8.3:39902971-39902990	None:intergenic	35.0%
CTGTTCTGATACTATCTTCT+TGG	+	chr8.3:39903090-39903109	MS.gene25274:intron	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8.3	gene	39902893	39904009	39902893	ID=MS.gene25274
chr8.3	mRNA	39902893	39904009	39902893	ID=MS.gene25274.t1;Parent=MS.gene25274
chr8.3	exon	39902893	39903007	39902893	ID=MS.gene25274.t1.exon1;Parent=MS.gene25274.t1
chr8.3	CDS	39902893	39903007	39902893	ID=cds.MS.gene25274.t1;Parent=MS.gene25274.t1
chr8.3	exon	39903126	39903380	39903126	ID=MS.gene25274.t1.exon2;Parent=MS.gene25274.t1
chr8.3	CDS	39903126	39903380	39903126	ID=cds.MS.gene25274.t1;Parent=MS.gene25274.t1
chr8.3	exon	39903783	39904009	39903783	ID=MS.gene25274.t1.exon3;Parent=MS.gene25274.t1
chr8.3	CDS	39903783	39904009	39903783	ID=cds.MS.gene25274.t1;Parent=MS.gene25274.t1

Gene Sequence

>MS.gene25274

ATGTTGTTAGAGTCTATAAATGCAACAAGTAAGAAAGTTGAGGAGATCATAGAAAAAGTTAATGATGATGTAATTCCAGGAGATATTCCAATTCGTATTGAGGAGCATTTATCAGGTTATATGTTTTTTCAATTGATCAATGAAATTTATCATAGCATATAAATGGAAAATGATGTAATTTCCTGATATTTTGTGTTCTGTTCTGATACTATCTTCTTGGTGTGTTGTTTTAGCTCTAAATCTTAGGTGCATTGAACGACTATACGGTGACCATGGGCTTGATGTGATGGAAGTGTTGAAAAAGAATGCATCTCTAGCCTTGCCGGTGATATTAACCCGCCTGAAGCAGAAACAGGAGGAGTGGGCAAGATGCCGTGCAGAGTTTAATATAGTTTGGGCTGATGTATATGCGAAGAATTATCATAAATCACTTGATCATCGAAGCTTTTACTTTAAGCAGCAGGATACAAAAAGCTTGAGCACTAAAGGTAAAATATTAATTTTCTGTCCAATAACACAATTCTTGATTCTTTTTAACGGTATGGCCCTCTTTAACAACCTTTAAATAGTAAGTTATTTAACATCCATTGAAAGAAAATATTTGATTTAATCCTTTTAATATAGTTAAGAAATATTTAAAATTAGTTTAGGGAATAAAGTATACTTAATTAATTTAATGTAGTTAGTTAAGAACACAGCCCAAACCAATATTAGCAGGGCGTGGTGGGCATTTTAGACAAGATGTTGCTCTCCGATATCCATAAACTCTTGAATAAGGAAGGGGATACGGAGGATCTAAATGAATATTTGCGTGAGTGTTTTTGTCTGAGAATTGCTTTCTTCATGCTTGCCTCTTTTCATTATTTGTTGACATTGTTTTTATTTTGTAGAACCAATGTTGCCCATGACGACTTCTGAGAGAAACAAATACTTGGCTGAAGTGAAGGTGAGGAAGGCAGCCGAGGGATATGCTACCAGTGATCCCGCCGGTGTAACTCATCGTAAAGCAAAGAGAAAAGACATTATTTCAAAGGATGGTGCTGTTACTGTTCAGGTTGATACGGAGCTTCGAGCAGGCTGTCGACGGGACTGGAGCGGAGATTGTGGATTCTCCTAA

Protein sequence

>MS.gene25274.t1

MLLESINATSKKVEEIIEKVNDDVIPGDIPIRIEEHLSALNLRCIERLYGDHGLDVMEVLKKNASLALPVILTRLKQKQEEWARCRAEFNIVWADVYAKNYHKSLDHRSFYFKQQDTKSLSTKEPMLPMTTSERNKYLAEVKVRKAAEGYATSDPAGVTHRKAKRKDIISKDGAVTVQVDTELRAGCRRDWSGDCGFS