Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25350.t1 | PNX85476.1 | 80.4 | 199 | 37 | 1 | 1 | 197 | 211 | 409 | 1.40E-89 | 339.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25350.t1 | Q9M2N5 | 36.7 | 215 | 125 | 4 | 1 | 214 | 491 | 695 | 1.3e-35 | 151.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25350.t1 | A0A2K3M3W2 | 80.4 | 199 | 37 | 1 | 1 | 197 | 211 | 409 | 9.9e-90 | 339.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049711 | MS.gene25350 | 0.819809 | 9.56E-53 | -1.69E-46 |
| MS.gene049825 | MS.gene25350 | 0.809193 | 2.14E-50 | -1.69E-46 |
| MS.gene051279 | MS.gene25350 | 0.815806 | 7.66E-52 | -1.69E-46 |
| MS.gene051719 | MS.gene25350 | 0.812185 | 4.81E-51 | -1.69E-46 |
| MS.gene051720 | MS.gene25350 | 0.803844 | 2.87E-49 | -1.69E-46 |
| MS.gene051744 | MS.gene25350 | 0.809038 | 2.31E-50 | -1.69E-46 |
| MS.gene052463 | MS.gene25350 | 0.800064 | 1.72E-48 | -1.69E-46 |
| MS.gene052519 | MS.gene25350 | 0.803211 | 3.88E-49 | -1.69E-46 |
| MS.gene053599 | MS.gene25350 | 0.804871 | 1.75E-49 | -1.69E-46 |
| MS.gene05365 | MS.gene25350 | 0.802807 | 4.71E-49 | -1.69E-46 |
| MS.gene053678 | MS.gene25350 | 0.8159 | 7.29E-52 | -1.69E-46 |
| MS.gene054651 | MS.gene25350 | 0.805526 | 1.28E-49 | -1.69E-46 |
| MS.gene05686 | MS.gene25350 | 0.804746 | 1.86E-49 | -1.69E-46 |
| MS.gene057102 | MS.gene25350 | 0.812172 | 4.85E-51 | -1.69E-46 |
| MS.gene058625 | MS.gene25350 | 0.801138 | 1.04E-48 | -1.69E-46 |
| MS.gene058635 | MS.gene25350 | 0.808027 | 3.79E-50 | -1.69E-46 |
| MS.gene059015 | MS.gene25350 | 0.800939 | 1.14E-48 | -1.69E-46 |
| MS.gene059625 | MS.gene25350 | 0.833638 | 4.75E-56 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25350.t1 | AT3G42170 | 38.021 | 192 | 110 | 3 | 1 | 191 | 491 | 674 | 6.26e-41 | 148 |
| MS.gene25350.t1 | AT3G42170 | 38.021 | 192 | 110 | 3 | 1 | 191 | 469 | 652 | 6.55e-41 | 148 |
| MS.gene25350.t1 | AT1G18560 | 27.861 | 201 | 116 | 7 | 2 | 191 | 467 | 649 | 3.95e-13 | 68.6 |
Find 38 sgRNAs with CRISPR-Local
Find 45 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCAGCTTCACTCTTCAAAA+TGG | 0.170935 | 8.3:+40069109 | None:intergenic |
| AACGGAGTTGGATCATTATC+TGG | 0.259746 | 8.3:-40069669 | MS.gene25350:CDS |
| TGCAGAAATTTGATAAATAT+TGG | 0.280085 | 8.3:-40069973 | MS.gene25350:CDS |
| CCACATCAGAATATCAAAAT+TGG | 0.289304 | 8.3:+40069622 | None:intergenic |
| TTTGGATCCAAGATACAAAA+TGG | 0.298966 | 8.3:-40069915 | MS.gene25350:CDS |
| GATGGGAGTTGCTACTGTTT+TGG | 0.320873 | 8.3:-40069933 | MS.gene25350:CDS |
| TCAGAAGCTACTGAAGTAAT+AGG | 0.323940 | 8.3:+40069536 | None:intergenic |
| ATATCAAAATTGGCACTTCT+AGG | 0.340733 | 8.3:+40069632 | None:intergenic |
| TTTGCAATTTCTTGCAATGT+TGG | 0.382104 | 8.3:+40069575 | None:intergenic |
| AAGATCTTACATTCATCATC+AGG | 0.417940 | 8.3:+40069238 | None:intergenic |
| TCAGCTTCACTCTTCAAAAT+GGG | 0.432393 | 8.3:+40069110 | None:intergenic |
| TAGCAACTCCCATCATATCC+TGG | 0.454437 | 8.3:+40069942 | None:intergenic |
| GCTAATTCTGTGGCTAATGA+TGG | 0.459373 | 8.3:-40069752 | MS.gene25350:CDS |
| ACTCTGTTGAGCAAGTTAAG+AGG | 0.474739 | 8.3:-40069853 | MS.gene25350:CDS |
| GAGTGTCACCCAGGATATGA+TGG | 0.476745 | 8.3:-40069951 | MS.gene25350:CDS |
| CTCATCGTAGTCGACTTCAT+TGG | 0.485204 | 8.3:-40069478 | MS.gene25350:CDS |
| ATATGGAGCAAGGAGAATAA+TGG | 0.491041 | 8.3:-40069419 | MS.gene25350:intron |
| TAGAACATCATTATATAAAA+CGG | 0.495038 | 8.3:-40069687 | MS.gene25350:CDS |
| ATCATTATATAAAACGGAGT+TGG | 0.499119 | 8.3:-40069681 | MS.gene25350:CDS |
| TAAAGAAATGGCTAAGAAGA+TGG | 0.502305 | 8.3:-40069996 | None:intergenic |
| CTTTGATGTGTGCTAGAAGT+TGG | 0.508597 | 8.3:-40069442 | MS.gene25350:CDS |
| ACTTCATTGGACAACCTTGG+AGG | 0.521822 | 8.3:-40069465 | MS.gene25350:CDS |
| GAATCAGCATTCAGTACAAG+TGG | 0.537506 | 8.3:-40069515 | MS.gene25350:CDS |
| TCGACTTCATTGGACAACCT+TGG | 0.544964 | 8.3:-40069468 | MS.gene25350:CDS |
| AGCAACTCCCATCATATCCT+GGG | 0.551068 | 8.3:+40069943 | None:intergenic |
| TGTGTGCTAGAAGTTGGATA+TGG | 0.560174 | 8.3:-40069436 | MS.gene25350:CDS |
| TAAATATTGGAGTGTCACCC+AGG | 0.565226 | 8.3:-40069960 | MS.gene25350:CDS |
| ACGGAGTTGGATCATTATCT+GGG | 0.571432 | 8.3:-40069668 | MS.gene25350:CDS |
| CTGATGTGGTGGAAGTTGAA+TGG | 0.578791 | 8.3:-40069608 | MS.gene25350:CDS |
| ATTATGATGCATATGTCAAG+AGG | 0.590157 | 8.3:-40069721 | MS.gene25350:CDS |
| AAGTTCAATCAAGACACTTG+TGG | 0.595420 | 8.3:-40069788 | MS.gene25350:CDS |
| AGACACTTGTGGTGAATCGT+CGG | 0.609844 | 8.3:-40069777 | MS.gene25350:CDS |
| AGTGTCACCCAGGATATGAT+GGG | 0.628166 | 8.3:-40069950 | MS.gene25350:CDS |
| AGCACACATCAAAGCCTCCA+AGG | 0.630649 | 8.3:+40069451 | None:intergenic |
| TAGAAGTTGGATATGGAGCA+AGG | 0.637735 | 8.3:-40069429 | MS.gene25350:CDS |
| ATAGGTGAAATATTGACTAG+TGG | 0.646439 | 8.3:-40069141 | MS.gene25350:intron |
| ATCGTCGGAAGCTAATTCTG+TGG | 0.696219 | 8.3:-40069762 | MS.gene25350:CDS |
| CAATGAAGTCGACTACGATG+AGG | 0.702001 | 8.3:+40069479 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ACTTGTATTTTTTATTATAT+AGG | - | chr8.3:40069946-40069965 | MS.gene25350:CDS | 10.0% |
| !! | AAACATTAAAGTTAGTAAAT+TGG | + | chr8.3:40069918-40069937 | None:intergenic | 15.0% |
| !! | TAGAACATCATTATATAAAA+CGG | - | chr8.3:40069418-40069437 | MS.gene25350:intron | 15.0% |
| !!! | TACTATTTTGAAAAATTGTA+TGG | - | chr8.3:40069224-40069243 | MS.gene25350:intron | 15.0% |
| !! | ATTAAAGTTAGTAAATTGGA+AGG | + | chr8.3:40069914-40069933 | None:intergenic | 20.0% |
| !! | CTTTCAATTTACATATCTTA+AGG | - | chr8.3:40069726-40069745 | MS.gene25350:CDS | 20.0% |
| !! | TGCAGAAATTTGATAAATAT+TGG | - | chr8.3:40069132-40069151 | MS.gene25350:CDS | 20.0% |
| ! | ATCATTATATAAAACGGAGT+TGG | - | chr8.3:40069424-40069443 | MS.gene25350:CDS | 25.0% |
| ! | GGAAGGTAGTATTAAATAAA+TGG | + | chr8.3:40069897-40069916 | None:intergenic | 25.0% |
| AAGATCTTACATTCATCATC+AGG | + | chr8.3:40069870-40069889 | None:intergenic | 30.0% | |
| ACTGCATTTGCATTATTTAC+AGG | - | chr8.3:40069789-40069808 | MS.gene25350:CDS | 30.0% | |
| ATATCAAAATTGGCACTTCT+AGG | + | chr8.3:40069476-40069495 | None:intergenic | 30.0% | |
| ATTATGATGCATATGTCAAG+AGG | - | chr8.3:40069384-40069403 | MS.gene25350:intron | 30.0% | |
| CCACATCAGAATATCAAAAT+TGG | + | chr8.3:40069486-40069505 | None:intergenic | 30.0% | |
| TTTGCAATTTCTTGCAATGT+TGG | + | chr8.3:40069533-40069552 | None:intergenic | 30.0% | |
| TTTGGATCCAAGATACAAAA+TGG | - | chr8.3:40069190-40069209 | MS.gene25350:intron | 30.0% | |
| ! | ATAGGTGAAATATTGACTAG+TGG | - | chr8.3:40069964-40069983 | MS.gene25350:CDS | 30.0% |
| !! | ATTTTGATATTCTGATGTGG+TGG | - | chr8.3:40069486-40069505 | MS.gene25350:CDS | 30.0% |
| !! | CCAATTTTGATATTCTGATG+TGG | - | chr8.3:40069483-40069502 | MS.gene25350:CDS | 30.0% |
| AAGTTCAATCAAGACACTTG+TGG | - | chr8.3:40069317-40069336 | MS.gene25350:intron | 35.0% | |
| ATATGGAGCAAGGAGAATAA+TGG | - | chr8.3:40069686-40069705 | MS.gene25350:CDS | 35.0% | |
| TCAGAAGCTACTGAAGTAAT+AGG | + | chr8.3:40069572-40069591 | None:intergenic | 35.0% | |
| ! | AGCAACTCCATTTTGTATCT+TGG | + | chr8.3:40069200-40069219 | None:intergenic | 35.0% |
| AACGGAGTTGGATCATTATC+TGG | - | chr8.3:40069436-40069455 | MS.gene25350:CDS | 40.0% | |
| ACGGAGTTGGATCATTATCT+GGG | - | chr8.3:40069437-40069456 | MS.gene25350:CDS | 40.0% | |
| ACTCTGTTGAGCAAGTTAAG+AGG | - | chr8.3:40069252-40069271 | MS.gene25350:CDS | 40.0% | |
| CTTTGATGTGTGCTAGAAGT+TGG | - | chr8.3:40069663-40069682 | MS.gene25350:CDS | 40.0% | |
| GAATCAGCATTCAGTACAAG+TGG | - | chr8.3:40069590-40069609 | MS.gene25350:CDS | 40.0% | |
| GCTAATTCTGTGGCTAATGA+TGG | - | chr8.3:40069353-40069372 | MS.gene25350:intron | 40.0% | |
| TAGAAGTTGGATATGGAGCA+AGG | - | chr8.3:40069676-40069695 | MS.gene25350:CDS | 40.0% | |
| TGTGTGCTAGAAGTTGGATA+TGG | - | chr8.3:40069669-40069688 | MS.gene25350:CDS | 40.0% | |
| ! | TAAATATTGGAGTGTCACCC+AGG | - | chr8.3:40069145-40069164 | MS.gene25350:intron | 40.0% |
| ACTTCATTGGACAACCTTGG+AGG | - | chr8.3:40069640-40069659 | MS.gene25350:CDS | 45.0% | |
| AGACACTTGTGGTGAATCGT+CGG | - | chr8.3:40069328-40069347 | MS.gene25350:intron | 45.0% | |
| AGCAACTCCCATCATATCCT+GGG | + | chr8.3:40069165-40069184 | None:intergenic | 45.0% | |
| AGTGTCACCCAGGATATGAT+GGG | - | chr8.3:40069155-40069174 | MS.gene25350:intron | 45.0% | |
| ATCGTCGGAAGCTAATTCTG+TGG | - | chr8.3:40069343-40069362 | MS.gene25350:intron | 45.0% | |
| CAATGAAGTCGACTACGATG+AGG | + | chr8.3:40069629-40069648 | None:intergenic | 45.0% | |
| TAGCAACTCCCATCATATCC+TGG | + | chr8.3:40069166-40069185 | None:intergenic | 45.0% | |
| TCGACTTCATTGGACAACCT+TGG | - | chr8.3:40069637-40069656 | MS.gene25350:CDS | 45.0% | |
| ! | CTCATCGTAGTCGACTTCAT+TGG | - | chr8.3:40069627-40069646 | MS.gene25350:CDS | 45.0% |
| ! | CTGATGTGGTGGAAGTTGAA+TGG | - | chr8.3:40069497-40069516 | MS.gene25350:CDS | 45.0% |
| ! | GATGGGAGTTGCTACTGTTT+TGG | - | chr8.3:40069172-40069191 | MS.gene25350:intron | 45.0% |
| AGCACACATCAAAGCCTCCA+AGG | + | chr8.3:40069657-40069676 | None:intergenic | 50.0% | |
| GAGTGTCACCCAGGATATGA+TGG | - | chr8.3:40069154-40069173 | MS.gene25350:intron | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 40069116 | 40070011 | 40069116 | ID=MS.gene25350 |
| chr8.3 | mRNA | 40069116 | 40070011 | 40069116 | ID=MS.gene25350.t1;Parent=MS.gene25350 |
| chr8.3 | exon | 40069420 | 40070011 | 40069420 | ID=MS.gene25350.t1.exon1;Parent=MS.gene25350.t1 |
| chr8.3 | CDS | 40069420 | 40070011 | 40069420 | ID=cds.MS.gene25350.t1;Parent=MS.gene25350.t1 |
| chr8.3 | exon | 40069248 | 40069316 | 40069248 | ID=MS.gene25350.t1.exon2;Parent=MS.gene25350.t1 |
| chr8.3 | CDS | 40069248 | 40069316 | 40069248 | ID=cds.MS.gene25350.t1;Parent=MS.gene25350.t1 |
| chr8.3 | exon | 40069116 | 40069159 | 40069116 | ID=MS.gene25350.t1.exon3;Parent=MS.gene25350.t1 |
| chr8.3 | CDS | 40069116 | 40069159 | 40069116 | ID=cds.MS.gene25350.t1;Parent=MS.gene25350.t1 |
| Gene Sequence |
| Protein sequence |
