Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26249.t1 | AFK46684.1 | 91.7 | 276 | 21 | 2 | 1 | 276 | 1 | 274 | 1.60E-100 | 375.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26249.t1 | P55871 | 65.9 | 276 | 85 | 5 | 1 | 274 | 1 | 269 | 1.5e-80 | 300.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26249.t1 | I3T2E2 | 91.7 | 276 | 21 | 2 | 1 | 276 | 1 | 274 | 1.1e-100 | 375.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene26249 | MS.gene061898 | PPI |
| MS.gene26249 | MS.gene063366 | PPI |
| MS.gene09852 | MS.gene26249 | PPI |
| MS.gene26249 | MS.gene060216 | PPI |
| MS.gene025997 | MS.gene26249 | PPI |
| MS.gene26249 | MS.gene22877 | PPI |
| MS.gene26249 | MS.gene039110 | PPI |
| MS.gene26249 | MS.gene018560 | PPI |
| MS.gene26249 | MS.gene63721 | PPI |
| MS.gene066163 | MS.gene26249 | PPI |
| MS.gene26249 | MS.gene058030 | PPI |
| MS.gene040581 | MS.gene26249 | PPI |
| MS.gene26249 | MS.gene024538 | PPI |
| MS.gene26249 | MS.gene003452 | PPI |
| MS.gene26249 | MS.gene050556 | PPI |
| MS.gene52139 | MS.gene26249 | PPI |
| MS.gene26249 | MS.gene64519 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26249.t1 | MTR_5g075530 | 92.029 | 276 | 20 | 2 | 1 | 276 | 49 | 322 | 1.74e-162 | 454 |
| MS.gene26249.t1 | MTR_3g467620 | 78.467 | 274 | 51 | 5 | 1 | 274 | 1 | 266 | 3.33e-132 | 375 |
| MS.gene26249.t1 | MTR_5g075530 | 92.529 | 174 | 12 | 1 | 1 | 174 | 49 | 221 | 6.29e-90 | 266 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26249.t1 | AT5G20920 | 66.545 | 275 | 83 | 4 | 1 | 273 | 1 | 268 | 5.69e-116 | 334 |
| MS.gene26249.t1 | AT5G20920 | 66.545 | 275 | 83 | 4 | 1 | 273 | 1 | 268 | 5.69e-116 | 334 |
| MS.gene26249.t1 | AT5G20920 | 66.545 | 275 | 82 | 5 | 1 | 273 | 1 | 267 | 1.15e-114 | 330 |
| MS.gene26249.t1 | AT3G07920 | 62.745 | 153 | 48 | 2 | 124 | 272 | 17 | 164 | 1.94e-59 | 186 |
| MS.gene26249.t1 | AT5G01940 | 50.704 | 142 | 65 | 3 | 120 | 260 | 72 | 209 | 5.32e-39 | 136 |
| MS.gene26249.t1 | AT5G01940 | 50.704 | 142 | 65 | 3 | 120 | 260 | 104 | 241 | 8.80e-39 | 136 |
| MS.gene26249.t1 | AT5G01940 | 48.077 | 156 | 72 | 4 | 120 | 268 | 72 | 225 | 5.56e-37 | 131 |
Find 57 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGAATTCCTGCAAAACTCTT+TGG | 0.299200 | 5.4:-58790269 | None:intergenic |
| TTGGTTGTGAAGGGAAGATT+TGG | 0.327299 | 5.4:+58790245 | MS.gene26249:CDS |
| TGGAGAGGCAGTTGAAGATT+TGG | 0.342163 | 5.4:+58782486 | MS.gene26249:CDS |
| CTTTGCAGCCTCGTTATCCT+TGG | 0.346996 | 5.4:+58782659 | MS.gene26249:CDS |
| TGATGAATCAGTGGATAAAT+TGG | 0.362248 | 5.4:+58782124 | MS.gene26249:CDS |
| CGCCGATCTCCAGCAAGATC+TGG | 0.373236 | 5.4:-58789720 | None:intergenic |
| GATGAATATGTCATTTGTAT+TGG | 0.394913 | 5.4:+58790537 | MS.gene26249:intron |
| GCAACAAAACCAGCTTTGAT+TGG | 0.396441 | 5.4:-58790727 | None:intergenic |
| TGTACTAGTGGTTATATAGA+TGG | 0.407956 | 5.4:+58790212 | MS.gene26249:CDS |
| TATTGCTTGCAGTGTGGATC+AGG | 0.417838 | 5.4:+58790691 | MS.gene26249:intron |
| TTGTATTGGATGCAAGAGTC+TGG | 0.425485 | 5.4:+58790551 | MS.gene26249:CDS |
| GAACATAATCCAGATCTTGC+TGG | 0.451726 | 5.4:+58789711 | MS.gene26249:CDS |
| TATAGATGGACAGCAAAGAT+TGG | 0.470808 | 5.4:+58790226 | MS.gene26249:CDS |
| TATTCACTTGCAGTTTCTGA+TGG | 0.471762 | 5.4:+58782276 | MS.gene26249:intron |
| AAGAAAGCCATAACATGGTC+TGG | 0.472766 | 5.4:-58790176 | None:intergenic |
| TGAAACACCAAGTGGTTTGA+AGG | 0.477271 | 5.4:+58780908 | MS.gene26249:CDS |
| TTTGCAGCCTCGTTATCCTT+GGG | 0.480164 | 5.4:+58782660 | MS.gene26249:CDS |
| CTTCTTTCTCCGGTGTGAGA+AGG | 0.497603 | 5.4:+58790602 | MS.gene26249:CDS |
| CAGCCTCGTTATCCTTGGGA+AGG | 0.501422 | 5.4:+58782664 | MS.gene26249:CDS |
| AGAACCGTCTCTTCTTTCTC+CGG | 0.503608 | 5.4:+58790592 | MS.gene26249:CDS |
| TCGACCGCCAGACCATGTTA+TGG | 0.515326 | 5.4:+58790169 | MS.gene26249:CDS |
| ATCCAGATCTTGCTGGAGAT+CGG | 0.530264 | 5.4:+58789718 | MS.gene26249:CDS |
| TTGCTCGTGTTGGCGGCAGA+AGG | 0.531864 | 5.4:+58790746 | MS.gene26249:CDS |
| TCTGGACACATTACTTTCAA+AGG | 0.537856 | 5.4:+58790569 | MS.gene26249:CDS |
| TGACCGTGATTATACATATG+AGG | 0.537992 | 5.4:+58782690 | MS.gene26249:CDS |
| TCAGTTGCTCCAATCAAAGC+TGG | 0.541836 | 5.4:+58790718 | MS.gene26249:CDS |
| GAGAAGATAGAAAATCTGTC+AGG | 0.544015 | 5.4:+58782149 | MS.gene26249:CDS |
| GTGCCTTCACGGCGAACTCG+AGG | 0.547950 | 5.4:-58789762 | None:intergenic |
| CCTCCTCGAGTTCGCCGTGA+AGG | 0.548479 | 5.4:+58789759 | MS.gene26249:CDS |
| ATTCACTTGCAGTTTCTGAT+GGG | 0.549550 | 5.4:+58782277 | MS.gene26249:intron |
| TCTTCAACTGCCTCTCCACT+AGG | 0.552585 | 5.4:-58782481 | None:intergenic |
| TTTGGTACAGCTTCTTGGCA+GGG | 0.553643 | 5.4:+58789671 | MS.gene26249:intron |
| ACTTCCTCCTTCAAACCACT+TGG | 0.569420 | 5.4:-58780915 | None:intergenic |
| ATCGGCGAAGAACAGTTATG+AGG | 0.576484 | 5.4:+58789736 | MS.gene26249:CDS |
| AGCCTCGTTATCCTTGGGAA+GGG | 0.579244 | 5.4:+58782665 | MS.gene26249:CDS |
| CAGCAAAGATTGGTTGTGAA+GGG | 0.579584 | 5.4:+58790236 | MS.gene26249:CDS |
| ACAGCAAAGATTGGTTGTGA+AGG | 0.582650 | 5.4:+58790235 | MS.gene26249:CDS |
| GAGGCAGTTGAAGATTTGGA+TGG | 0.585613 | 5.4:+58782490 | MS.gene26249:CDS |
| CTTGCTGAACTATGTACTAG+TGG | 0.601332 | 5.4:+58790200 | MS.gene26249:CDS |
| AGTAAGGCCAAATCATGCCT+CGG | 0.608563 | 5.4:-58790773 | None:intergenic |
| TACTGCAGATGATGAATCAG+TGG | 0.608690 | 5.4:+58782115 | MS.gene26249:CDS |
| TTAAATGATATTGATGAGAG+TGG | 0.613941 | 5.4:+58782457 | MS.gene26249:CDS |
| CCTCCTCATATGTATAATCA+CGG | 0.618081 | 5.4:-58782693 | None:intergenic |
| CTCCCTTCCCAAGGATAACG+AGG | 0.620050 | 5.4:-58782667 | None:intergenic |
| CCTTCACGGCGAACTCGAGG+AGG | 0.625429 | 5.4:-58789759 | None:intergenic |
| CAAGCAAGAAAGCCATAACA+TGG | 0.625960 | 5.4:-58790181 | None:intergenic |
| AATACTTACTGATATATCGT+CGG | 0.636337 | 5.4:-58790292 | None:intergenic |
| TCACGGTCACTCCCTTCCCA+AGG | 0.642597 | 5.4:-58782676 | None:intergenic |
| GAAAATTCACCTTCTCACAC+CGG | 0.642926 | 5.4:-58790611 | None:intergenic |
| AACACCAAGTGGTTTGAAGG+AGG | 0.644979 | 5.4:+58780911 | MS.gene26249:CDS |
| ATTGATGAGAGTGGTCCTAG+TGG | 0.645228 | 5.4:+58782466 | MS.gene26249:CDS |
| TGAGAGTGGTCCTAGTGGAG+AGG | 0.666586 | 5.4:+58782471 | MS.gene26249:CDS |
| CACACCGGAGAAAGAAGAGA+CGG | 0.669379 | 5.4:-58790596 | None:intergenic |
| TGGCGGCAGAAGGAATGCCG+AGG | 0.670535 | 5.4:+58790756 | MS.gene26249:CDS |
| CCGTGATTATACATATGAGG+AGG | 0.685858 | 5.4:+58782693 | MS.gene26249:CDS |
| AAAGCCATAACATGGTCTGG+CGG | 0.709853 | 5.4:-58790173 | None:intergenic |
| ATGGCAGATGAAACACCAAG+TGG | 0.740265 | 5.4:+58780900 | MS.gene26249:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 58780900 | 58790783 | 58780900 | ID=MS.gene26249 |
| chr5.4 | mRNA | 58780900 | 58790783 | 58780900 | ID=MS.gene26249.t1;Parent=MS.gene26249 |
| chr5.4 | exon | 58780900 | 58780944 | 58780900 | ID=MS.gene26249.t1.exon1;Parent=MS.gene26249.t1 |
| chr5.4 | CDS | 58780900 | 58780944 | 58780900 | ID=cds.MS.gene26249.t1;Parent=MS.gene26249.t1 |
| chr5.4 | exon | 58782059 | 58782170 | 58782059 | ID=MS.gene26249.t1.exon2;Parent=MS.gene26249.t1 |
| chr5.4 | CDS | 58782059 | 58782170 | 58782059 | ID=cds.MS.gene26249.t1;Parent=MS.gene26249.t1 |
| chr5.4 | exon | 58782289 | 58782347 | 58782289 | ID=MS.gene26249.t1.exon3;Parent=MS.gene26249.t1 |
| chr5.4 | CDS | 58782289 | 58782347 | 58782289 | ID=cds.MS.gene26249.t1;Parent=MS.gene26249.t1 |
| chr5.4 | exon | 58782442 | 58782511 | 58782442 | ID=MS.gene26249.t1.exon4;Parent=MS.gene26249.t1 |
| chr5.4 | CDS | 58782442 | 58782511 | 58782442 | ID=cds.MS.gene26249.t1;Parent=MS.gene26249.t1 |
| chr5.4 | exon | 58782626 | 58782714 | 58782626 | ID=MS.gene26249.t1.exon5;Parent=MS.gene26249.t1 |
| chr5.4 | CDS | 58782626 | 58782714 | 58782626 | ID=cds.MS.gene26249.t1;Parent=MS.gene26249.t1 |
| chr5.4 | exon | 58789681 | 58789826 | 58789681 | ID=MS.gene26249.t1.exon6;Parent=MS.gene26249.t1 |
| chr5.4 | CDS | 58789681 | 58789826 | 58789681 | ID=cds.MS.gene26249.t1;Parent=MS.gene26249.t1 |
| chr5.4 | exon | 58790163 | 58790305 | 58790163 | ID=MS.gene26249.t1.exon7;Parent=MS.gene26249.t1 |
| chr5.4 | CDS | 58790163 | 58790305 | 58790163 | ID=cds.MS.gene26249.t1;Parent=MS.gene26249.t1 |
| chr5.4 | exon | 58790538 | 58790623 | 58790538 | ID=MS.gene26249.t1.exon8;Parent=MS.gene26249.t1 |
| chr5.4 | CDS | 58790538 | 58790623 | 58790538 | ID=cds.MS.gene26249.t1;Parent=MS.gene26249.t1 |
| chr5.4 | exon | 58790703 | 58790783 | 58790703 | ID=MS.gene26249.t1.exon9;Parent=MS.gene26249.t1 |
| chr5.4 | CDS | 58790703 | 58790783 | 58790703 | ID=cds.MS.gene26249.t1;Parent=MS.gene26249.t1 |
| Gene Sequence |
| Protein sequence |
