AlfalfaGEDB | MS.gene27697

M. sativa cultivar XinJiangDaYe / MS.gene27697

KEGG: K12614
GO: GO:0000166 GO:0003674 GO:0003676 GO:0005488 GO:0005524 GO:0008144 GO:0017076 GO:0030554 GO:0032553 GO:0032555 GO:0032559 GO:0035639 GO:0036094 GO:0043167 GO:0043168 GO:0097159 GO:0097367 GO:1901265 GO:1901363
PFAM: PF00270.32

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene27697.t1	RHN46904.1	100	78	0	0	1	78	132	209	1.90E-35	157.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene27697.t1	Q8RXK6	94.9	78	4	0	1	78	124	201	2.5e-36	152.1

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene27697.t1	G7L3J8	100.0	78	0	0	1	78	133	210	1.4e-35	157.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049142	MS.gene27697	0.827208	1.78E-54	-1.69E-46
MS.gene049159	MS.gene27697	0.812036	5.19E-51	-1.69E-46
MS.gene050551	MS.gene27697	0.800443	1.44E-48	-1.69E-46
MS.gene051102	MS.gene27697	0.808614	2.84E-50	-1.69E-46
MS.gene051198	MS.gene27697	0.811962	5.39E-51	-1.69E-46
MS.gene051744	MS.gene27697	0.8094	1.93E-50	-1.69E-46
MS.gene051800	MS.gene27697	0.839436	1.59E-57	-1.69E-46
MS.gene052307	MS.gene27697	0.800293	1.54E-48	-1.69E-46
MS.gene052516	MS.gene27697	0.800753	1.24E-48	-1.69E-46
MS.gene052609	MS.gene27697	0.84837	6.40E-60	-1.69E-46
MS.gene053079	MS.gene27697	0.822032	2.94E-53	-1.69E-46
MS.gene053621	MS.gene27697	0.803216	3.88E-49	-1.69E-46
MS.gene054576	MS.gene27697	0.800093	1.69E-48	-1.69E-46
MS.gene055469	MS.gene27697	0.807458	5.01E-50	-1.69E-46
MS.gene055513	MS.gene27697	0.804761	1.85E-49	-1.69E-46
MS.gene055864	MS.gene27697	0.822063	2.90E-53	-1.69E-46
MS.gene056308	MS.gene27697	0.806332	8.66E-50	-1.69E-46
MS.gene056507	MS.gene27697	0.845453	4.02E-59	-1.69E-46
MS.gene057869	MS.gene27697	0.836336	9.93E-57	-1.69E-46
MS.gene057915	MS.gene27697	0.826585	2.50E-54	-1.69E-46
MS.gene058010	MS.gene27697	0.821442	4.03E-53	-1.69E-46
MS.gene058289	MS.gene27697	0.829839	4.11E-55	-1.69E-46
MS.gene058647	MS.gene27697	0.824593	7.42E-54	-1.69E-46
MS.gene058649	MS.gene27697	0.800343	1.51E-48	-1.69E-46
MS.gene058919	MS.gene27697	0.839137	1.90E-57	-1.69E-46
MS.gene059439	MS.gene27697	0.814815	1.27E-51	-1.69E-46
MS.gene059570	MS.gene27697	0.804893	1.74E-49	-1.69E-46
MS.gene060167	MS.gene27697	0.808332	3.26E-50	-1.69E-46
MS.gene06052	MS.gene27697	0.831448	1.66E-55	-1.69E-46
MS.gene060637	MS.gene27697	0.811509	6.76E-51	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene27697.t1	MTR_7g076800	100.000	78	0	0	1	78	133	210	9.39e-49	160
MS.gene27697.t1	MTR_7g076830	100.000	78	0	0	1	78	135	212	1.10e-48	160
MS.gene27697.t1	MTR_7g076820	97.436	78	2	0	1	78	142	219	1.30e-47	157
MS.gene27697.t1	MTR_0915s0010	100.000	58	0	0	21	78	1	58	6.69e-36	122
MS.gene27697.t1	MTR_3g107420	50.000	62	31	0	10	71	48	109	6.19e-16	70.5
MS.gene27697.t1	MTR_1g012420	51.613	62	30	0	10	71	48	109	9.35e-16	70.1
MS.gene27697.t1	MTR_3g107420	50.000	62	31	0	10	71	48	109	3.25e-15	68.6
MS.gene27697.t1	MTR_4g010565	50.000	62	31	0	10	71	125	186	4.04e-15	68.6
MS.gene27697.t1	MTR_4g079320	44.444	63	35	0	10	72	20	82	6.28e-15	67.8
MS.gene27697.t1	MTR_2g041140	44.286	70	39	0	1	70	133	202	3.39e-14	65.9
MS.gene27697.t1	MTR_3g049990	44.118	68	38	0	3	70	146	213	4.29e-13	62.8
MS.gene27697.t1	MTR_1g083130	44.262	61	34	0	10	70	27	87	6.21e-13	62.4
MS.gene27697.t1	MTR_2g438110	35.366	82	49	1	1	78	22	103	5.42e-12	59.7
MS.gene27697.t1	MTR_7g109720	40.984	61	36	0	10	70	233	293	1.50e-11	58.5
MS.gene27697.t1	MTR_1g061970	41.892	74	40	2	1	71	72	145	1.53e-11	58.2
MS.gene27697.t1	MTR_8g027770	47.059	51	27	0	20	70	212	262	3.19e-11	57.4
MS.gene27697.t1	MTR_8g027775	45.098	51	28	0	20	70	113	163	8.25e-11	56.2
MS.gene27697.t1	MTR_4g049910	42.857	70	37	2	5	71	51	120	8.80e-11	56.2
MS.gene27697.t1	MTR_8g018400	43.750	64	36	0	8	71	40	103	9.54e-11	56.2
MS.gene27697.t1	MTR_8g027780	45.098	51	28	0	20	70	117	167	9.91e-11	55.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene27697.t1	AT4G00660	94.872	78	4	0	1	78	124	201	9.21e-47	155
MS.gene27697.t1	AT4G00660	94.872	78	4	0	1	78	124	201	9.21e-47	155
MS.gene27697.t1	AT3G61240	88.462	78	9	0	1	78	117	194	2.35e-43	146
MS.gene27697.t1	AT3G61240	88.462	78	9	0	1	78	117	194	2.35e-43	146
MS.gene27697.t1	AT2G45810	88.462	78	9	0	1	78	147	224	8.01e-43	145
MS.gene27697.t1	AT5G11200	48.387	62	32	0	10	71	48	109	7.95e-15	67.8
MS.gene27697.t1	AT5G11200	48.387	62	32	0	10	71	48	109	7.99e-15	67.8
MS.gene27697.t1	AT5G11170	48.387	62	32	0	10	71	48	109	1.48e-14	67.0
MS.gene27697.t1	AT5G11200	48.387	62	32	0	10	71	48	109	1.48e-14	67.0
MS.gene27697.t1	AT4G16630	45.312	64	35	0	7	70	166	229	1.28e-13	64.3
MS.gene27697.t1	AT1G77030	46.032	63	34	0	10	72	30	92	1.53e-13	63.9
MS.gene27697.t1	AT5G14610	45.714	70	35	1	10	76	171	240	1.46e-12	61.2
MS.gene27697.t1	AT5G14610	45.714	70	35	1	10	76	171	240	1.51e-12	61.2
MS.gene27697.t1	AT4G34910	44.262	61	34	0	10	70	48	108	1.62e-12	61.2
MS.gene27697.t1	AT3G19760	37.681	69	43	0	10	78	37	105	2.18e-12	60.8
MS.gene27697.t1	AT5G14610	45.714	70	35	1	10	76	163	232	1.06e-11	58.9
MS.gene27697.t1	AT5G14610	45.714	70	35	1	10	76	163	232	1.07e-11	58.9
MS.gene27697.t1	AT5G08620	44.262	61	34	0	10	70	82	142	2.70e-11	57.8
MS.gene27697.t1	AT5G08610	45.902	61	33	0	10	70	384	444	2.78e-11	57.8
MS.gene27697.t1	AT2G47330	39.344	61	37	0	10	70	230	290	2.93e-11	57.8
MS.gene27697.t1	AT5G63630	44.262	61	34	0	10	70	56	116	4.79e-11	57.0
MS.gene27697.t1	AT5G63630	44.262	61	34	0	10	70	250	310	5.21e-11	57.0
MS.gene27697.t1	AT3G53110	40.541	74	41	2	1	71	84	157	9.93e-11	56.2

Find 22 sgRNAs with CRISPR-Local

Find 23 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGCATTCCAATTGCCCTTAC+TGG	0.269182	7.3:+29403933	MS.gene27697:CDS
CAATTGGAATGCTTTCTTCT+TGG	0.291211	7.3:-29403923	None:intergenic
TTTGAAGCGTGAGCTACTAA+TGG	0.365162	7.3:+29403863	MS.gene27697:CDS
TTGAAGCGTGAGCTACTAAT+GGG	0.399247	7.3:+29403864	MS.gene27697:CDS
TCACTGCCAGTAAGGGCAAT+TGG	0.428644	7.3:-29403939	None:intergenic
TGCATTTAGCATTCCTGCAT+TGG	0.429066	7.3:+29404001	MS.gene27697:CDS
CAAGATAACAATATTATTCA+AGG	0.434070	7.3:+29404035	MS.gene27697:CDS
CCAGGACGTGACAGCAACTA+AGG	0.441774	7.3:+29403818	None:intergenic
GAATGCTTTCTTCTTGGATA+GGG	0.449165	7.3:-29403917	None:intergenic
CTGGCAGTGACATTCTTGCT+AGG	0.452575	7.3:+29403952	MS.gene27697:CDS
GGAATGCTTTCTTCTTGGAT+AGG	0.455771	7.3:-29403918	None:intergenic
CAAGAATGTCACTGCCAGTA+AGG	0.471837	7.3:-29403947	None:intergenic
AACTAAGGGAAATGAGTTTG+AGG	0.503108	7.3:+29403833	MS.gene27697:CDS
CTTTCTTCTTGGATAGGGGA+AGG	0.528864	7.3:-29403912	None:intergenic
TATATGAGAAGGGGTTTGAA+AGG	0.529351	7.3:+29403889	MS.gene27697:CDS
AAGAATGTCACTGCCAGTAA+GGG	0.548067	7.3:-29403946	None:intergenic
TGGCAGTGACATTCTTGCTA+GGG	0.556476	7.3:+29403953	MS.gene27697:CDS
ACTAATGGGAATATATGAGA+AGG	0.574201	7.3:+29403878	MS.gene27697:CDS
CTAATGGGAATATATGAGAA+GGG	0.574494	7.3:+29403879	MS.gene27697:CDS
AATGCTTTCTTCTTGGATAG+GGG	0.593841	7.3:-29403916	None:intergenic
CAGGACGTGACAGCAACTAA+GGG	0.624954	7.3:+29403819	None:intergenic
TAATGGGAATATATGAGAAG+GGG	0.642760	7.3:+29403880	MS.gene27697:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	CAAGATAACAATATTATTCA+AGG	+	chr7.3:29404035-29404054	MS.gene27697:CDS	20.0%
	ACTAATGGGAATATATGAGA+AGG	+	chr7.3:29403878-29403897	MS.gene27697:CDS	30.0%
	CTAATGGGAATATATGAGAA+GGG	+	chr7.3:29403879-29403898	MS.gene27697:CDS	30.0%
	TAATGGGAATATATGAGAAG+GGG	+	chr7.3:29403880-29403899	MS.gene27697:CDS	30.0%
!!!	TGATCAATTTTTTCCAATGC+AGG	-	chr7.3:29404017-29404036	None:intergenic	30.0%
	AACTAAGGGAAATGAGTTTG+AGG	+	chr7.3:29403833-29403852	MS.gene27697:CDS	35.0%
	AATGCTTTCTTCTTGGATAG+GGG	-	chr7.3:29403919-29403938	None:intergenic	35.0%
	ATTCTTGCTAGGGCTAAAAA+TGG	+	chr7.3:29403963-29403982	MS.gene27697:CDS	35.0%
	CAATTGGAATGCTTTCTTCT+TGG	-	chr7.3:29403926-29403945	None:intergenic	35.0%
	GAATGCTTTCTTCTTGGATA+GGG	-	chr7.3:29403920-29403939	None:intergenic	35.0%
	TATATGAGAAGGGGTTTGAA+AGG	+	chr7.3:29403889-29403908	MS.gene27697:CDS	35.0%
	AAGAATGTCACTGCCAGTAA+GGG	-	chr7.3:29403949-29403968	None:intergenic	40.0%
	GGAATGCTTTCTTCTTGGAT+AGG	-	chr7.3:29403921-29403940	None:intergenic	40.0%
	TGCATTTAGCATTCCTGCAT+TGG	+	chr7.3:29404001-29404020	MS.gene27697:CDS	40.0%
	TTGAAGCGTGAGCTACTAAT+GGG	+	chr7.3:29403864-29403883	MS.gene27697:CDS	40.0%
!	TTTGAAGCGTGAGCTACTAA+TGG	+	chr7.3:29403863-29403882	MS.gene27697:CDS	40.0%
	AGCATTCCAATTGCCCTTAC+TGG	+	chr7.3:29403933-29403952	MS.gene27697:CDS	45.0%
	CAAGAATGTCACTGCCAGTA+AGG	-	chr7.3:29403950-29403969	None:intergenic	45.0%
	CTTTCTTCTTGGATAGGGGA+AGG	-	chr7.3:29403915-29403934	None:intergenic	45.0%
	GCTAGGGCTAAAAATGGAAC+AGG	+	chr7.3:29403969-29403988	MS.gene27697:CDS	45.0%
	TGGCAGTGACATTCTTGCTA+GGG	+	chr7.3:29403953-29403972	MS.gene27697:CDS	45.0%
	CTGGCAGTGACATTCTTGCT+AGG	+	chr7.3:29403952-29403971	MS.gene27697:CDS	50.0%
	TCACTGCCAGTAAGGGCAAT+TGG	-	chr7.3:29403942-29403961	None:intergenic	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.3	gene	29403822	29404056	29403822	ID=MS.gene27697
chr7.3	mRNA	29403822	29404056	29403822	ID=MS.gene27697.t1;Parent=MS.gene27697
chr7.3	exon	29403822	29404056	29403822	ID=MS.gene27697.t1.exon1;Parent=MS.gene27697.t1
chr7.3	CDS	29403822	29404056	29403822	ID=cds.MS.gene27697.t1;Parent=MS.gene27697.t1

Gene Sequence	>MS.gene27697 GACGTGACAGCAACTAAGGGAAATGAGTTTGAGGATTACTTTTTGAAGCGTGAGCTACTAATGGGAATATATGAGAAGGGGTTTGAAAGGCCTTCCCCTATCCAAGAAGAAAGCATTCCAATTGCCCTTACTGGCAGTGACATTCTTGCTAGGGCTAAAAATGGAACAGGCAAAACAGCTGCATTTAGCATTCCTGCATTGGAAAAAATTGATCAAGATAACAATATTATTCAAG
Protein sequence	>MS.gene27697.t1 DVTATKGNEFEDYFLKRELLMGIYEKGFERPSPIQEESIPIALTGSDILARAKNGTGKTAAFSIPALEKIDQDNNIIQ